Aquaculture最新文献

{"title":"Dietary creatine enhances neurodevelopment and cognitive performance in juvenile largemouth bass (Micropterus salmoides): Implications for aquaculture efficiency and conservation strategies","authors":"Haodong Yu ,&nbsp;Jiajie Xue ,&nbsp;Yuxin Li ,&nbsp;Yaqi Shi ,&nbsp;Yinjuan Xiong ,&nbsp;Yukang Nie ,&nbsp;Abeer M. Hegazy ,&nbsp;Xuezhen Zhang","doi":"10.1016/j.aquaculture.2025.743184","DOIUrl":"10.1016/j.aquaculture.2025.743184","url":null,"abstract":"<div><div>Creatine contributes to cognition and brain health in mammalian. Neuronal regeneration in the teleost brain shows great potential throughout the life cycle, dietary creatine may be of potential value in aquatic animal brains. Therefore, in this study, 6 feeds with different creatine additions (0 %, 0.2 %, 0.5 %, 1 %, 2 %, and 4 %) were formulated and fed to juvenile largemouth bass (<em>Micropterus salmoides</em>) for 8 weeks. The brain creatine metabolism of largemouth bass was detected by Q-PCR, Western blot, and high-performance liquid chromatography (HPLC). Dietary creatine significantly increased creatine inventory, mRNA levels of brain-type creatine kinase, protein level of creatine transporter protein (SLC6A8) in the brain of largemouth bass. This indicated that exogenous creatine can effectively pass through the blood-brain barrier of largemouth bass and deposited in the brain. Western blot for relative quantification of protein levels, it was found in this study that creatine could elevate the protein levels of BDNF (Brain Derived Neurotrophic Factor) as well as phosphorylated activation of the AKT (Protein Kinase B) /mTOR (Mechanistic Target of Rapamycin Kinase) and the ERK (Extracellular Signal-Regulated Kinase)/p90RSK (90 KDa Ribosomal Protein S6 Kinase) in the largemouth bass brain to activate brain neurotrophin signaling pathways. Teleost can consistently produce new neurons in the adult brain. In this study, dietary creatine significantly upregulated neuro-related growth factors genes, indicating that dietary creatine promotes neurodevelopment and neurogenesis in the brain. In the present study, multiple experimental setups were used to assess fish behavior. Dietary creatine significantly increased the forward maximum acceleration of largemouth bass in open-field test; significantly increased the exploration time in new object recognition test (NORT); and significantly increased the mean swimming speed and forward maximum acceleration of largemouth bass in sociability test. In locomotor (swimming) capacity tests, the burst swimming speed after rest was significantly elevated, which possibly resulting in enhanced predation ability. Overall, these findings suggest that long-term dietary creatine promotes largemouth bass brain health and neurodevelopment, enhance cognitive, and motor performance. In the future, creatine may have multiple potential applications in aquaculture, such as enhancing the adaptability of stock enhancement fish after returning to nature, strengthening cognitive and motor abilities thereby increasing the economic benefits of fish rearing and aquaculture production.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743184"},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardizing CASA analysis to accurately assess sea urchin Paracentrotus lividus sperm motility","authors":"Sabrina Gacem ,&nbsp;Estefanía Paredes ,&nbsp;Sara Campos ,&nbsp;Francisco Sevilla ,&nbsp;Carles Soler ,&nbsp;Jesús Yániz ,&nbsp;Anthony Valverde ,&nbsp;Miguel A. Silvestre","doi":"10.1016/j.aquaculture.2025.743197","DOIUrl":"10.1016/j.aquaculture.2025.743197","url":null,"abstract":"<div><div>Although computer-assisted sperm analysis (CASA) system is widely applied for evaluating sperm motility in numerous vertebrate species, its application to sea urchin sperm remains comparatively underexplored, with limited data available on this invertebrate model. This study aimed to standardize the frame rate, type of counting chambers, extender, and dilution rate to use CASA system to evaluate sea urchin sperm motility. Semen samples were collected and diluted in artificial sea water alone (ASW) or supplemented with 0.3 % serum bovine albumin (ASW-BSA). The diluted semen was filled in 4 chamber types: drop displacement, Spermtrack® (SK) and R2D10 (R2); and capillary, Kubus® (KU); and SpermLide® (SL). Three dilutions ranging from low to high were studied. Sperm motility was evaluated using a commercial CASA system (AI Station v1.2; Sperm Analysis Technologies S.L., Buñol, Spain) that employs artificial intelligence for sperm recognition. Spermatozoa were immotile in drop displacement chambers when diluted in ASW; however total motility (TMOT) and progressivity (PMOT) did not differ significantly across chamber types when semen was diluted in ASW-BSA, independently of filling chamber type. KU chamber showed significantly higher velocity parameters compared to SL chamber when semen was diluted in ASW. However, these differences disappeared when in ASW-BSA medium, and straight line velocity (VSL) was even significantly higher in SL chamber. As dilution increased (i.e., lower cell concentration), sperm velocity increased, and linearity decreased. The optimal frame rate for accurate measurement of curvilinear velocity (VCL) was 510 fps, whereas lower frame rates (&lt; 360 fps) were sufficient for evaluating VSL and average path velocity (VAP). In conclusion, to optimally assess sea urchin sperm motility using CASA requires a frame rate of 510 fps, dilution in ASW-BSA to concentrations below 75 × 10⁶ sperm/mL and using capillary-filling chambers.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743197"},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of sex and reproductive protein biomarkers in conditioned water of the Sydney rock oyster","authors":"Saowaros Suwansa-ard ,&nbsp;Tianfang Wang ,&nbsp;Md Abu Zafar ,&nbsp;Michael Dove ,&nbsp;Wayne O'Connor ,&nbsp;Scott F. Cummins ,&nbsp;Abigail Elizur","doi":"10.1016/j.aquaculture.2025.743198","DOIUrl":"10.1016/j.aquaculture.2025.743198","url":null,"abstract":"<div><div>The Sydney rock oyster (SRO), <em>Saccostrea glomerata</em>, is one of the most important aquaculture species in Australian, and improving production techniques is essential for creating reliable, sustainable farming practices with increased profitability. Broodstock sex identification is a significant challenge since they do not display any external sexual dimorphism and current sex identification approaches rely on invasive and destructive techniques that compromise broodstock integrity. Therefore, this study investigated whether SROs secrete sex and reproductive stage-specific semiochemical protein profiles that could be used for sex identification. Following proteomic analysis of oyster-conditioned water at two different reproductive stages (ripe and spent) for each sex, we demonstrate that SROs do secrete a distinct sex and reproductive stage protein profile. Functional annotation revealed these secreted proteins are potentially involved in metabolic processes, immunity and chemical communication. Furthermore, semi-quantitative analysis facilitated identification of candidate female-specific protein biomarkers, with primary targets for further investigation being temptin and byssal protein-3-like proteins. Both proteins showed high gene expression in the oyster's digestive gland and were abundant in the conditioned water of females. In conclusion, this study has established that SROs release distinctive protein profiles, some of which may be utilized as biomarkers to infer sex and reproductive stage non-invasively. This foundational knowledge provides a basis for developing novel approaches for non-invasive detection of oyster sex, reproductive stage, and immune status, thereby offering significant benefits to aquaculture industry operations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743198"},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic potential of Isochrysis galbana microbiomes against the fish pathogen Vibrio anguillarum","authors":"Matilde Emídio Almeida,&nbsp;Dóra Smahajcsik,&nbsp;Shengda Zhang,&nbsp;Lone Gram","doi":"10.1016/j.aquaculture.2025.743196","DOIUrl":"10.1016/j.aquaculture.2025.743196","url":null,"abstract":"<div><div>The aquaculture industry is expanding; yet it is challenged by disease outbreaks, many of which are caused by pathogenic bacteria. Such infections can be controlled by antibiotics, however, this contributes to the development and spread of antimicrobial resistance (AMR) and therefore alternative strategies for disease control must be developed. The purpose of this study was to investigate the antibacterial potential of the microbiome associated with the live-feed microalgae <em>Isochrysis galbana.</em> A GFP-tagged highly virulent strain of <em>Vibrio anguillarum</em> (strain 90–11-286) was incubated with different fractions of the algae microbiome and the fluorescence signal was followed to screen for its inhibition. The microalgal microbiome was able to inhibit <em>V. anguillarum</em> and was dominated by <em>Alteromonadaceae</em> and <em>Rhodobacteraceae</em>. When testing isolated pure bacterial cultures, only <em>Phaeobacter</em> could inhibit the pathogen in monoculture, however, given its low prevalence in the microbiome, it was not likely the cause of pathogen inhibition. Co-cultures of <em>Halomonas</em> and <em>Ruegeria</em> were capable of inhibiting the low virulent <em>V. anguillarum</em> strain NB10, however could not inhibit the high-virulent <em>V. anguillarum</em> strain 90–11-286. This study demonstrates that the native microbiomes of microalgae from aquaculture environments hold anti-pathogen potential and highlights the possible benefits of using mixtures of bacteria rather than pure cultures for pathogen suppression.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743196"},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of MarR-deleted Streptococcus agalactiae as a live-attenuated vaccine candidate for Nile Tilapia (Oreochromis niloticus)","authors":"Tian-Yu Ma ,&nbsp;Feng-yang Li ,&nbsp;Chang-Song Wu ,&nbsp;Jian-Hao Lin ,&nbsp;Ming Chen ,&nbsp;Yong-An Zhang ,&nbsp;Hui Zeng","doi":"10.1016/j.aquaculture.2025.743195","DOIUrl":"10.1016/j.aquaculture.2025.743195","url":null,"abstract":"<div><div><em>Streptococcus agalactiae</em> is the dominant etiological agent inducing streptococcosis, which is the major threat to global tilapia aquaculture. Live-attenuated vaccines can induce robust cellular and humoral immunity, and have been proven to provide effective protection against <em>S. agalactiae</em>. Selecting an appropriate target is crucial for the development of attenuated vaccine. In this study, we constructed a mutant strain (Δ<em>marR</em>) lacking the gene encoding the multiple antibiotic resistance regulator (MarR) to evaluate its vaccine potential. Comparative survival assays revealed that the Δ<em>marR</em> exhibited significantly reduced blood survival relative to the wild-type (WT) HN016 strain. Additionally, the Δ<em>marR</em> showed decreased hemolytic activity, anti-phagocytosis ability, adhesion and invasion capabilities. More importantly, its pathogenicity in tilapia was markedly attenuated with an LD₅₀ value 356-fold higher than HN016. Furthermore, immunization by intracelomic injection with Δ<em>marR</em> conferred significant protection in tilapia against subsequent challenge with HN016 at 30 days post-vaccination, with 95 % vaccinated tilapia surviving for longer than 30 days and relative percent survival of 93.99 %. To investigate the immune response, we quantified immunity-related genes expression in head kidney and spleen. The results demonstrated that vaccination with Δ<em>marR</em> could induce a robust and sustained adaptive immune response in tilapia. Taken together, the findings indicate that the <em>marR</em> gene deletion causes significant attenuation of <em>S. agalactiae</em> and the Δ<em>marR</em> mutant emerges as a promising vaccine candidate to combat <em>S. agalactiae</em> infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743195"},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish pathogen armor: A review of antiphage defense systems in bacterial fish pathogens","authors":"Kaushika Olymon ,&nbsp;Nafeesah Kinoo ,&nbsp;Nitul Roy ,&nbsp;Venkata Rajesh Yella ,&nbsp;Valentina Teronpi ,&nbsp;Aditya Kumar","doi":"10.1016/j.aquaculture.2025.743192","DOIUrl":"10.1016/j.aquaculture.2025.743192","url":null,"abstract":"<div><div>Bacterial diseases remain a major bottleneck to sustainable aquaculture, and interest in bacteriophage (phage) therapy has surged accordingly. However, the efficacy and durability of phage interventions are constrained by the range of chromosomally encoded antiphage defense systems in fish-pathogenic bacteria. Here, we survey publicly available genomes to establish an aquaculture-centric landscape of these defenses and their implications for therapeutic design. Across 272 genomes representing 35 fish-pathogenic species, we identified 1,174 antiphage defense systems spanning 304 distinct defense types (e.g., CRISPR-Cas, restriction-modification, DISARM, BREX, CBASS). Core systems (restriction–modification and CRISPR-Cas) are widespread, while emerging systems show patchy, lineage-specific distributions. Genomic context analysis reveals frequent clustering into defense “islands” and recurrent co-occurrence patterns, suggesting modular acquisition and potential redundancy. To translate these findings, we compile a curated crosswalk of defense types versus known phage countermeasures (anti-CRISPRs, DNA mimic proteins, anti-CBASS factors, and others) and critically appraise evidence for phage therapy from laboratory to farm settings in aquaculture species. Together, these results (i) delineate defense repertoires most likely to impede single-phage treatments, (ii) highlight taxa where cocktail rotation or engineered anti-defense traits may be necessary, and (iii) provide a practical decision framework for candidate selection, monitoring, and stewardship. This study delivers a consolidated resource and actionable guidance for rational phage development and deployment against fish-pathogenic bacteria, supporting more resilient, evidence-based disease control in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743192"},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appropriate zinc requirements of Litopenaeus vannamei under low-salinity cultivation estimated by biokinetic modeling","authors":"Qi Yang ,&nbsp;Minjie Hu ,&nbsp;Lijun Ning ,&nbsp;Jian Wang ,&nbsp;Xun Wang","doi":"10.1016/j.aquaculture.2025.743193","DOIUrl":"10.1016/j.aquaculture.2025.743193","url":null,"abstract":"<div><div>The Pacific white shrimp (<em>Litopenaeus vannamei</em>) has become a popular species in low-salinity aquaculture in China, while its production efficiency remains limited compared to marine systems. Zinc (Zn), an essential mineral element, plays a critical role in shrimp growth. In the present study, we employed the stable isotope ⁶⁸Zn as a tracer to quantify Zn accumulation dynamics in L. <em>vannamei</em> reared at 5 ppt salinity. An optimized biokinetic model was developed to determine the precise Zn requirements in low-salinity conditions. The results demonstrate that dietary uptake made more contribution to Zn assimilation (&gt;50 %) than waterborne uptake under environmentally relevant dissolved Zn concentrations (5–20 μg L⁻¹). The biokinetic model estimated a daily net Zn flux of 1.16–1.27 μg g⁻¹ d⁻¹, corresponding to an appropriate dietary Zn concentration of 44–66 mg kg⁻¹ at ingestion rates of 2–3 %. These predictions were validated by a 30-day feeding trial, where shrimp fed diets supplemented with 40–80 mg kg⁻¹ Zn exhibited significantly improved growth performance and physiological responses (e.g., digestive and antioxidant enzyme activities). Overall, this study provides a mechanistic framework for optimizing Zn supplementation in shrimp feeds. The biokinetic approach offers an alternative to traditional methods, with broader applications for precise mineral nutrition.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743193"},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification and population genetic analysis of a three-way cross hybrid abalone","authors":"Haichen Zhang ,&nbsp;Qizhen Xiao ,&nbsp;Weihong Lin ,&nbsp;Zekun Huang ,&nbsp;Benben Miao ,&nbsp;Yang Gan ,&nbsp;Chengyun Li ,&nbsp;Caihuan Ke ,&nbsp;Xuan Luo ,&nbsp;Weiwei You","doi":"10.1016/j.aquaculture.2025.743189","DOIUrl":"10.1016/j.aquaculture.2025.743189","url":null,"abstract":"<div><div>Abalone is economically important shellfish with a worldwide distribution. China is the largest abalone producing and consuming country in the world. In China, interspecific hybrids are used in large-scale abalone production to improve growth performance and stress tolerance. Previous research has shown that the interspecific three-way crosses hybrid abalone (<em>H. gigantea</em>♀ × (<em>Haliotis discus hannai</em>♀ × <em>H. fulgens</em>♂) ♂, SS × DF)display heterosis for growth performance and thermal and hypoxia tolerance. However, systematic analyses of their genetic structure are still lacking. In this study, the population genetic structure of SS × DF and its parents were analysed using genome-wide SNPs. At the same time, specific SNPs of SS × DF were identified. The results showed that SS × DF populations were genetically closer to SS and exhibited intermediate levels of heterozygosity. A population-specific SNP locus was identified, and developed a PCR-based marker that achieved 100 % accuracy in distinguishing SS × DF from other populations. The genomic regions with high differentiation (zFst) were identified, and found that genes enriched in MAPK and Rho GTPase signaling pathways may contribute to the hybrid's superior tolerance to heat and hypoxia. These findings provide molecular evidence of successful three-way hybridization and offer valuable insights for breeding strategies and germplasm management in abalone aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743189"},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid purification-free LAMP assay for detection of largemouth Bass Ranavirus using minimally invasive samples","authors":"Chaoguang Wei ,&nbsp;Chenwei Yu ,&nbsp;Jinjie Zu ,&nbsp;Xinyang Li ,&nbsp;Yilan Bao ,&nbsp;Feibiao Pang ,&nbsp;Zhichao Wu ,&nbsp;Dan Xu ,&nbsp;Jinzhao Song ,&nbsp;Peng Hu","doi":"10.1016/j.aquaculture.2025.743190","DOIUrl":"10.1016/j.aquaculture.2025.743190","url":null,"abstract":"<div><div>Largemouth Bass Ranavirus (LMBV) causes severe mortality in largemouth bass (<em>Micropterus salmoides</em>), significantly threatening aquaculture sustainability. Conventional PCR-based methods for LMBV detection are resource-intensive, requiring specialized equipment, skilled personnel, and purified DNA samples, thus limiting their practical applications. Here, we established a simplified, purification-free loop-mediated isothermal amplification (LAMP) assay integrated with a portable fluorescence detection device (WeD-1) for rapid and sensitive detection of LMBV. Using an in vitro infection model, we demonstrated that crude lysates from minimally invasive samples, particularly peritoneal fluid, could be directly used in LAMP assays without nucleic acid purification. The entire detection workflow—from sample collection to result interpretation—was completed within 40  minutes (min). This approach provides a sensitive, rapid, and cost-effective diagnostic method for early detection of LMBV and can potentially be extended for rapid detection of other aquatic pathogens.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743190"},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid method to generate an all-female population in common carp (Cyprinus carpio) using the neomale carp of F0 population with targeted aromatase mutations","authors":"Yuting Xie ,&nbsp;Hongfei Bao ,&nbsp;Guanghui Chen ,&nbsp;Xuehui Li ,&nbsp;Yonglin Ruan ,&nbsp;Qiyong Lou ,&nbsp;Xia Jin ,&nbsp;Jiangyan He ,&nbsp;Tianshu Zhang ,&nbsp;Jian Wang ,&nbsp;Xueqiao Qian ,&nbsp;Gang Zhai ,&nbsp;Zhan Yin","doi":"10.1016/j.aquaculture.2025.743185","DOIUrl":"10.1016/j.aquaculture.2025.743185","url":null,"abstract":"<div><div>Teleosts are excellent models for elucidating the regulatory mechanisms of gonadal differentiation. Although the essential role of ovarian aromatase (encoded by <em>cyp19a1a</em>) in ovarian differentiation has been documented in several species of fish, this remains poorly understood in common carp (<em>Cyprinus carpio</em>) due to its long period of sexual maturity and the complexity of the multiple loci in its allotetraploid genome. Here, using the CRISPR/Cas9 strategy, some neomale carp with chimeric mutation (CM) of <em>cyp19a1aA</em>, <em>cyp19a1aB</em> or <em>cyp19a1aA</em>;<em>cyp19a1aB</em> were found in the F0 population at 1 year post-hatching (yph). The mutation ratios of <em>cyp19a1aA</em>, <em>cyp19a1aB</em>, or <em>cyp19a1aA</em>;<em>cyp19a1aB</em> were positively correlated with the ratio of sex reversal from female to male. Concentrations of estradiol were significantly decreased in female and neomale of chimaeras carp compared to wild-type (WT) control female carp. The progeny from artificial fertilization between WT female carp and these neomale carp were confirmed as all-female (AF) carp, as evidenced by genetic identification of male-specific DNA marker and histological analysis of progeny gonads. Finally, in comparison to the control group, which comprised both male and female carp reared in the same pond, AF carp at 4 months post-hatching (mph) demonstrated enhanced 12.06 % growth performance. In conclusion, we have shown that both Cyp19a1aA and Cyp19a1aB are required for estrogen synthesis, which is essential for ovarian differentiation in common carp. Therefore, a rapid method to generate AF populations using the F0 CM populations has been established by genetic manipulation targeting the loci of the <em>cyp19a1a</em> genes. The present study has revealed the non-redundant roles of <em>cyp19a1aA</em> and <em>cyp19a1aB</em> in ovarian differentiation and also provides an efficient and rapid method of controlling gonadal differentiation in fish without knowledge of the specific sex-determining gene (SDG) of the particular fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743185"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}