Aquaculture最新文献

{"title":"The microbial community composition of substrate biofilm limits the growth of indoor-cultured sea urchins (Strongylocentrotus intermedius)","authors":"Haifeng Huang ,&nbsp;Hongce Song ,&nbsp;Meiyun Dong ,&nbsp;Chaoyi Xie ,&nbsp;Yuxuan Zhang ,&nbsp;Kai Zhang ,&nbsp;Lei Wei ,&nbsp;Xiaotong Wang","doi":"10.1016/j.aquaculture.2025.742633","DOIUrl":"10.1016/j.aquaculture.2025.742633","url":null,"abstract":"<div><div>In benthic aquaculture, the use of substrates enhances farming efficiency and also induces microbial biofilm formation. The presence of biofilms is closely associated with the aquacultural environment and potentially influences the growth and development of aquatic animals. <em>Strongylocentrotus intermedius</em> is a highly valued species in aquaculture due to its commercially significant gonads. To date, however, few studies have investigated the associations between gut microbial community structures and functions in <em>S. intermedius</em> and substrate surface biofilms. In this study, we examined dynamic changes in the microbial communities on substrate biofilms in <em>S. intermedius</em> culture and assessed their potential effects on sea urchin health. Scanning electron microscopy, contact angle measurement, and quantitative analyses of biofilms revealed that biofilms on the surface of polyvinyl chloride (PVC) substrates gradually increased over time. Fourier-transform infrared spectrometry analysis indicated that PVC substrates gradually age with prolonged cultivation time. Microbial community analysis revealed a succession of dominant bacterial genera in PVC substrate biofilms, particularly those in genera with plastic-degradation ability, including <em>Pseudomonas</em>, <em>Cladosporium</em>, and <em>Alternaria</em>, which may promote PVC aging. Comparative microbial analyses revealed that <em>Vibrio</em> was highly abundant in <em>S. intermedius</em> grown in indoor and offshore culture environments, <em>Cobetia</em> and <em>Debaryomyces</em> were highly abundant in the intestine of offshore-cultured <em>S. intermedius</em>, and <em>Pseudoalteromonas</em>, <em>Candidatus_Hepatoplasma</em>, <em>Cladosporium</em>, and <em>Aspergillus</em> were highly abundant in the guts of indoor-cultured <em>S. intermedius</em>, among which only <em>Candidatus_Hepatoplasma</em> was not detected in PVC substrates. These differences are assumed to reflect the ecological interactions and nutritional requirements of <em>S. intermedius</em> in different habitats, as well as their adaptability to environmental change. Our research demonstrates that the relationship between the biofilms on sea urchin attachment bases and their gut microbiota must be better understood to optimise aquaculture. In particular, the role of beneficial microbes, notably fungi, within these attachment bases requires further investigation. Creating a beneficial bacterial biofilm can optimise the gut environment, improve the growth rate, and promote the overall health of sea urchins.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742633"},"PeriodicalIF":3.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of functional ncRNAs in the liver reveals a novel regulatory mechanism associated with the dynamic balance of growth and reproduction in Cynoglossus semilaevis","authors":"Kewen Yan ,&nbsp;Bao Shi ,&nbsp;Hanliang Cheng ,&nbsp;Yan Chen ,&nbsp;Daiqiang Zhang ,&nbsp;Yani Dong ,&nbsp;Jing Li ,&nbsp;Xinyu Zhao ,&nbsp;Meijun Tao","doi":"10.1016/j.aquaculture.2025.742630","DOIUrl":"10.1016/j.aquaculture.2025.742630","url":null,"abstract":"<div><div>Non-coding RNAs (ncRNAs) play crucial roles in the growth and reproduction of fish. However, the involvement of ncRNAs in the growth and reproductive balance of Chinese tongue sole (<em>Cynoglossus semilaevis</em>) from the late vitellogenesis stage to postovulation stage remains unexplored. Thus, to elucidate the regulatory mechanisms underlying the growth and reproductive balance regulated by ncRNAs, we performed whole transcriptome RNA sequencing of liver tissue at three ovarian stages, resulting in a total of nine libraries. In stages IV vs. V, 48 differentially expressed (DE) long non-coding RNAs (lncRNAs), 119 DE circular RNAs (circRNAs), 249 DE miRNAs, and 1797 DE mRNAs were identified. In stages IV vs. VI, we identified 209 DE lncRNAs, 417 DE circRNAs, 360 DE miRNAs, and 6760 DE mRNAs. In stages V vs. VI, we identified 133 DE lncRNAs, 376 DE circRNAs, 272 DE miRNAs, and 5129 DE mRNAs. Enrichment analysis revealed a significant enrichment of 116 DE mRNAs related to growth or reproduction in various pathways, including those related to arginine biosynthesis, steroid biosynthesis, steroid hormone biosynthesis, arginine and proline metabolism, and the insulin signalling pathway. Gene set enrichment analysis (GSEA) was further conducted to identify potential pathways related to growth-reproduction balance including the arginine biosynthesis pathway and steroid biosynthesis pathway. By utilizing <em>antisense</em>-, <em>cis</em>-, and <em>trans</em>-regulatory mechanisms, we have predicted that lncRNAs play regulatory roles in growth and reproduction-related genes, including <em>odc1-a</em>, <em>hsd11b2</em>, <em>fdft1</em>, <em>mapk8</em>, <em>irs2</em>, <em>sms</em>, <em>cyp24a1</em>, <em>crkl</em>, <em>flot2a</em>, <em>inppi1a</em>, <em>prkab1</em>, <em>prkaca</em>, <em>prkar2a</em>, <em>gls</em>, <em>pik3cg</em>, <em>glul</em>, <em>cyp1a1</em>, and <em>soat2</em>. By leveraging the network mechanism of competitive endogenous RNAs (ceRNAs), our study successfully predicted the intricate interactions among DE lncRNAs, DE circRNAs, DE miRNAs, and DE mRNAs including <em>acacb</em>, <em>amd1</em>, <em>cyp27b1</em>, <em>faxdc2</em>, <em>glul</em>, <em>pik3cg</em>, <em>prkab1</em>, and <em>rps6kb2</em>. A dual-fluorescence in situ hybridization assay revealed the colocalization of XR_522197.2 and <em>glul</em> in the liver parenchyma and around the central vein. The dual-luciferase reporter assay was employed to further demonstrate the regulatory relationships within the axis composed of TCON_00029307, novel_circ_008472, novel-m0618-5p, and <em>cyp27b1</em>. The comprehensive findings of this study outline the expression patterns of lncRNAs, circRNAs, and miRNAs in the liver of female <em>C. semilaevis</em>, providing a novel perspective that these three types of ncRNAs play critical roles in regulating the dynamic balance between growth and reproduction in this species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742630"},"PeriodicalIF":3.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA033033-mediated regulation of shell growth through octopamine signaling in Pinctada fucata martensii","authors":"Yong Liu ,&nbsp;Qi He ,&nbsp;Zhe Zheng ,&nbsp;Yuewen Deng","doi":"10.1016/j.aquaculture.2025.742631","DOIUrl":"10.1016/j.aquaculture.2025.742631","url":null,"abstract":"<div><div>Long non-coding RNAs (lncRNAs) play pivotal roles in numerous physiological processes by modulating various signaling pathways through activation or repression mechanisms. G protein-coupled receptors (GPCRs), including octopamine receptors (OARs), are integral to physiological regulation, particularly through the transmission of catecholamine hormone signals. However, the specific roles and mechanisms of lncRNAs in GPCR-mediated pathways remain unclear. In this study, we identified a novel lncRNA, lncRNA033033, in the pearl oyster <em>Pinctada fucata martensii</em>. This lncRNA exhibited high expression in the mantle tissue and was significantly associated with growth traits in adult oysters. And lncRNA033033 displayed increased expression at early trochophore and eyespot larval stages with the primary exoskeleton formation. Following shell damage, the expression of lncRNA033033 was initially downregulated and subsequently upregulated in the mantle over a two-day period, indicating its involvement in the transition from stress response to recovery. LncRNA033033 was found to interact with OAR, as detected by RNA pulldown. Over-expression of lncRNA033033 in vitro potentially competed with octopamine for binding to PfOAR, and inhibited the cAMP-mediated cAMP-response element binding protein (CREB) signaling pathway induced by OA. Together, these findings suggest that lncRNA033033 is essential for shell growth, larval development, and stress response in <em>P. f. martensii</em>, providing new insights into the regulatory functions of lncRNA via GPCR signaling pathways.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742631"},"PeriodicalIF":3.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing growth, pigmentation, and health in Penaeus vannamei through zeaxanthin supplementation","authors":"Yucai Xue ,&nbsp;Samia Fawzy ,&nbsp;Meiqin Wu ,&nbsp;Ganfeng Yi ,&nbsp;Amina S. Moss ,&nbsp;Xuxiong Huang ,&nbsp;Weilong Wang","doi":"10.1016/j.aquaculture.2025.742628","DOIUrl":"10.1016/j.aquaculture.2025.742628","url":null,"abstract":"<div><div>Carotenoids are essential dietary components in aquaculture, widely used to enhance pigmentation, improve stress tolerance, and boost immune responses in aquatic animals. An 8-week feeding trial was conducted to evaluate the effects of varying dietary levels of zeaxanthin (Zea) on the growth performance, carotenoid composition, antioxidant capacity, intestinal microbiota, biochemical parameters, and immune-physiological responses of <em>Penaeus vannamei</em>. A total of 600 juveniles (initial weight: 1.21 ± 0.12 g) were fed with five iso‑nitrogenous and isolipidic experimental diets containing incremental levels of Zea (0, 50, 100, 200, and 400 mg/kg diet). The results showed that dietary Zea supplementation significantly improved carotenoid deposition in shrimp tissues. Additionally, changes in carotenoid levels within the shrimp body demonstrated significant positive correlations (<em>P</em> &lt; 0.05) with growth performance indicators, survival, feed conversion ratio, DHA deposition, hemato-immunological enzymes (alkaline phosphatase, lysozyme, acid phosphatase), antioxidant capacities (total antioxidant capacity and peroxidase), digestive enzymes activities, hypoxia tolerance response (LT₅₀), and redness and yellowness parameters (<em>a</em>^<em>⁎</em>and <em>b</em>^<em>⁎</em>). While dietary Zea did not affect the total bacterial load, it significantly influenced intestinal microbiota diversity indices (Simpson, Shannon, and Chao) and relative abundance of dominant phyla, such as Proteobacteria. Furthermore, a comprehensive evaluation using Technique for Order Preference by Similarity to Ideal Solution (TOPSIS), combined with broken-line regression analysis, revealed that dietary Zea supplementation between 110.5 and 187.4 mg/kg diet resulted in distributed improvements across multiple parameters, including growth, antioxidant capacity, stress tolerance, enzyme activity, digestion, and body coloration. These findings highlight the potential of Zea as an alternative to traditional carotenoids in aquaculture. This study provides valuable insights into optimizing shrimp feed formulation, enhancing sustainability, and reducing production costs while maintaining high product quality.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742628"},"PeriodicalIF":3.9,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A death domain-containing NLR receptor promotes lytic cell death by interacting with DD-caspase in Vibrio splendidus-challenged Apostichopus japonicus","authors":"Yuxin Liu ,&nbsp;Kaiyu Chen ,&nbsp;Chenghua Li","doi":"10.1016/j.aquaculture.2025.742625","DOIUrl":"10.1016/j.aquaculture.2025.742625","url":null,"abstract":"<div><div>The NLR family serves as pattern recognition receptors that activate the innate immune response by sensing pathogenic factors. These receptors mediate pyroptosis and promote the release of inflammatory factors, thereby effectively defending against pathogen invasion. Invertebrates possess NLRs exhibiting diverse domain architectures, where distinct structural combinations contribute to functional diversification. However, the mechanistic basis of these evolutionary innovations remains poorly characterized. In this study, we cloned and identified a novel NLR from the <em>Apostichopus japonicus</em>, designated as AjDD-NLR, which is characterized by the combination of a death domain (DD) at its N-terminus, a nucleotide-binding and oligomerization domain (NACHT) in the middle, and a small number of leucine-rich repeat (LRR) motifs at its C-terminus, and this receptor lacked the recruitment domain (CARD) or pyrin domain (PYD) that are typical of common cytoplasmic NLR-NT. The AjDD-NLR expression was siginificantly induced in <em>Apostichopus japonicus</em> following <em>Vibrio splendidus</em> infection and in LPS-stimulated coelomocytes. PAMP binding assays revealed that AjDD-NLR recognizes diverse PAMPs via its C-terminal LRR domain.Mechanistically, a novel caspase (named AjDD-caspase), which also contains a DD domain, was found to be recruited by AjDD-NLR. Moreover, it was found that the lytic cell death promoted by overexpression of AjDD-NLR could be blocked by interfering with AjDD-caspase, which clearly demonstrated that AjDD-NLR regulates lytic cell death through the AjDD-caspase pathway. In conclusion, these results supported that AjDD-NLR regulates lytic cell death by recruiting AjDD-caspase.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742625"},"PeriodicalIF":3.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a novel birnavirus strain in diseased Siniperca chuatsi and transcriptome profiling of virus-infected MFSB cells","authors":"Hongye Cui ,&nbsp;Haiyue Chen ,&nbsp;Yuhui Wang ,&nbsp;Yaoda Wang ,&nbsp;Tianmei Hu ,&nbsp;Liwen Xu ,&nbsp;Baozhou Wu ,&nbsp;Shuguang Hao ,&nbsp;Xiong Hu ,&nbsp;Jiping Zhang ,&nbsp;Yu Liu ,&nbsp;Weiwei Zeng","doi":"10.1016/j.aquaculture.2025.742618","DOIUrl":"10.1016/j.aquaculture.2025.742618","url":null,"abstract":"<div><div>In August 2023, an outbreak of disease occurred in a pond containing <em>Siniperca chuatsi</em> fingerlings at a farm in Foshan, Guangdong province, resulting in a mortality rate exceeding 90 %. In this study, a novel birnavirus strain was isolated and identified from the diseased <em>Siniperca chuatsi</em> using cell culture, electron microscopy (EM), immunofluorescence assay (IFA), whole genome sequencing, and genetic evolutionary analysis. The virus was provisionally named <em>Siniperca chuatsi</em> Birnavirus FS202308 (SCBV-FS202308). Additionally, we analyzed the transcriptomic expression of SCBV-202308-infected mandarin fish spinal bulb (MFSB) cells. Results showed the MFSB cells inoculated with tissue homogenate exhibited typical cytopathic effects (CPE) post-infection. EM revealed a large number of icosahedral virion particles with a diameter approximately 60 nm were observed in the cells. Whole genome sequencing and phylogenetic analyses confirmed that the virus is a member of the family <em>Birnavirus</em>, specifically classified within the genus <em>Blosnavirus</em>. IFA utilizing antibodies against VP2 of SCBV and against IPNV revealed strong green fluorescent signals in the infected cells. Additionally, the challenge test demonstrated that all virus-inoculated <em>S. chuatsi</em> were successfully reinfected with SCBV-FS202308, resulting in a final mortality rate of approximately 20 %. Moreover, transcriptome analysis identified 2882 up-regulated and 3101 down-regulated genes in SCBV-FS202308-infected cells compared to the uninfected MFSB cells. Additionally, 522 genes were differentially enriched in pathways associated with immune signaling, such as the MAPK signaling, PI3K-Akt signaling, and Rap1 signaling pathways. This suggests that the virus elicits a robust immune response upon infecting host cells. This study represents the first report on the isolation, identification, and characterization of birnavirus from <em>Siniperca chuatsi.</em></div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742618"},"PeriodicalIF":3.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic study of Vibrio parahaemolyticus phage Фvp140 in Litopenaeus vannamei following oral administration","authors":"Di Fang ,&nbsp;Laizhuang Jin ,&nbsp;Qianhui Song ,&nbsp;Xiong Zou ,&nbsp;Lei Feng ,&nbsp;Letian Li ,&nbsp;Hui Zhang ,&nbsp;Kun Hu ,&nbsp;Mao Lin ,&nbsp;Wenhong Fang","doi":"10.1016/j.aquaculture.2025.742619","DOIUrl":"10.1016/j.aquaculture.2025.742619","url":null,"abstract":"<div><div>Bacteriophages (phages) are natural predators of bacteria, and have been shown to be potential alternatives to antibiotics in the prevention and treatment of bacterial infections of shrimp. In order to understand whether phages can effectively reach the site of infection, the pharmacokinetics of <em>Vibrio parahaemolyticus</em> phage Фvp140 in <em>Litopenaeus vannamei</em> was investigated following oral administration by gavage and via medicated feed. Whether administered by gavage or via medicated feed, phage Фvp140 could be rapidly distributed to various tissues of shrimp. The phage titer was the highest in the intestine, followed by the hepatopancreas, whose phage titer was about 10 % of that in the intestine, and only a small amount of phages could enter the circulatory system. A simple exponential decay model could effectively describe the elimination of phages in each tissue, and the higher the dose, the faster the elimination rate. The elimination rate of the phage in the hepatopancreas was the fastest, while the elimination rate of the phage in the intestine was similar to (by oral gavage) or faster than (via medicated feed) that in hemolymph. After oral gavage at three doses, the phage titers in shrimp tissues showed a dose-dependent relationship. This study found that after oral administration of phages, a large number of phages could directly into the hepatopancreas. Although the phage eliminated quickly，the continuous oral administration of phages in shrimp via medicated feed enabled the maintenance of a high level of phage titers in the hepatopancreas. This study provided pharmacokinetic evidence for the application of phage in shrimp farming and laid the foundation for further optimization of bacteriophage therapy.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742619"},"PeriodicalIF":3.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Horizontal and vertical transmission of covert mortality nodavirus in the marine fish","authors":"Tingting Xu ,&nbsp;Jitao Xia ,&nbsp;Chong Wang ,&nbsp;Yingxia Li ,&nbsp;Jun Li ,&nbsp;Qingli Zhang","doi":"10.1016/j.aquaculture.2025.742620","DOIUrl":"10.1016/j.aquaculture.2025.742620","url":null,"abstract":"<div><div>Covert mortality nodavirus (CMNV) is a vital emerging virus in aquaculture issued by World Organization for Animal Health recently. It can infect teleost fish, but the mode of transmission remains unclear. Therefore, this study investigated the potential of vertical and horizontal transmission of CMNV in marine fish using the marine model organism of marine medaka <em>Oryzias melastigma</em>. First, the clinical symptoms, viral load and histopathological changes of marine medaka artificially infected by intraperitoneal injection infection were consistent with those of fish species naturally infected with CMNV, indicating that marine medaka is an ideal model organism for CMNV infection. Subsequently, RT-nPCR analysis demonstrated a 10.5 % CMNV positivity rate in cohabitation-challenged marine medaka, with viral loads ranging from 3.28 × 10² to 3.21 × 10³ copies per gram of tissue. Histopathological analysis revealed severe lesions occurred in the brain, eye, kidney, and intestine of infected individuals, with positive hybridization signals of CMNV RNA probes were detected at necrotic sites in <em>in situ</em> hybridization (ISH) assay. These results provide strong evidence for the horizontal transmission of CMNV through waterborne in marine medaka. In addition, RT-nPCR and ISH analysis showed that ovarian tissue, testis tissue, fertilized eggs/embryos, and offspring fish from artificially infected parental marine medaka all tested positive for CMNV, demonstrating that vertical transmission can occur through either parent. The results of this study provide compelling evidence for both vertical and horizontal modes of transmission of CMNV in marine medaka.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742620"},"PeriodicalIF":3.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient digestibility and faecal characteristics in rainbow trout, Oncorhynchus mykiss reared at different salinities and fed diets with varying protein to energy ratios","authors":"Satya Prakash ,&nbsp;Roel M. Maas ,&nbsp;Ahmad Yani ,&nbsp;Fotini Kokou ,&nbsp;Øystein Sæle ,&nbsp;Antony J. Prabhu Philip ,&nbsp;Johan W. Schrama","doi":"10.1016/j.aquaculture.2025.742621","DOIUrl":"10.1016/j.aquaculture.2025.742621","url":null,"abstract":"<div><div>Knowledge on quantity and characteristics of faecal waste produced by cultured fish species is important to develop appropriate waste management strategy in aquaculture. The current study aimed to evaluate the impact of dietary protein-to-energy ratio (P:E) on faecal waste production and faecal characteristics of rainbow trout (<em>Oncorhynchus mykiss</em>), reared at different water salinity. Two diets (HPE diet- high protein to energy ratio diet; LPE diet- low protein to energy ratio diet) varying in protein-to-energy ratio (P:E) were produced while keeping the fat-to-starch ratio constant. Each diet was allocated to 6 replicate tanks and feeding was done restrictively. Experiment was divided into two phases: in the first phase, fish were reared at 1.5 ppt salinity for 4 weeks, followed by a second phase where salinity increased weekly to 6, 12, 18 and 24 and 30 ppt. Nutrient digestibility, faecal waste production, faecal characteristics were measured at 1.5 ppt, 12 ppt, 24 ppt and 30 ppt, respectively. Both salinity and diet influenced the digestibility of macronutrients, including crude protein, crude fat, and starch. No interaction effect between diet and salinity was observed for crude protein and crude fat digestibility. Both crude protein and starch digestibility were higher at HPE diet than at LPE diet, whereas for fat digestibility, the case was opposite. The amount of faecal waste produced and their removal efficiency was influenced by the interaction effect between diet and salinity. The amount of faecal waste produced was higher at the LPE diet than at the HPE diet. At both diets, the amount of waste produced increased with salinity, but more so with the LPE diet. Visual observation and analysis of particle size distribution (PSD) data showed greater presence of faecal fines at LPE diet, while the impact of salinity on faecal PSD was less distinct. The amount of non-removed faeces was more than double at LPE diet compared to HPE diet, with no clear trend observed with increasing salinity. In conclusion, the amount of faecal waste produced and removal efficiency are influenced by diet and water salinity, but also by their interaction.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742621"},"PeriodicalIF":3.9,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring community energy cum Aquavoltaics in Taiwan","authors":"Sin Yee Tan ,&nbsp;Kenneth Bicol Dy ,&nbsp;Ching-Cheng Chang ,&nbsp;Shih-Hsun Hsu","doi":"10.1016/j.aquaculture.2025.742602","DOIUrl":"10.1016/j.aquaculture.2025.742602","url":null,"abstract":"<div><div>Aquavoltaics, the fusion of aquaculture and solar electricity production, has been deemed as a key strategy for achieving energy transition by the government in Taiwan. Southern Taiwan, which serves as the region's primary agricultural and aquaculture production base, simultaneously has a significant potential for ground-mounted and rooftop solar photovoltaics. Given the urgency being accorded to energy transition across the globe, the feasibility of different solutions that can contribute towards renewable energy development must be investigated.</div><div>This study has conducted a cost-benefit analysis of different forms of aquavoltaics in Taiwan after accounting for government policies to incentivize independent renewable energy producers such as feed-in-tariff, subsidies, and renewable energy certificates, which complements past feasibility studies. In addition, we explore the possibility of combining a social innovation—community energy—with aquavoltaics. The results reveal that, in general, roof-mounted aquavoltaics are more profitable than ground-mounted or floating photovoltaic installations because of greater allowable installation capacity. In terms of operational model, a cooperative-type of community energy outperforms the landowner-run and company-run aquavoltaics. The results are largely driven by the availability of subsidies for cooperative-type community energies. Hence, this study recommends “community energy” as a feasible setup for aquavoltaics. However, the law must be amended to allow an aquavoltaics facility to sell electricity to the community without having to change into an electricity firm.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742602"},"PeriodicalIF":3.9,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}