Aquaculture最新文献

{"title":"Control of salmon lice through optical delousing in commercial salmonid aquaculture","authors":"Martin Worm ,&nbsp;Jan Bulla ,&nbsp;John Harald Pettersen ,&nbsp;Benedikt Frenzl","doi":"10.1016/j.aquaculture.2025.742910","DOIUrl":"10.1016/j.aquaculture.2025.742910","url":null,"abstract":"<div><div>Parasitic salmon lice are a major challenge for salmonid aquaculture in Norway. The development of resistance against most available medicines has favored the implementation of mechanical treatment alternatives, many of which have negative implications for the health and welfare of farmed fish. Optical delousing is a controlling measure that continuously reduces salmon lice in commercial sea cages. It relies on machine vision and object detection algorithms to detect salmon lice and remove them with a targeted laser pulse on freely swimming fish. Although this technology is increasingly adopted by the farming industry, an empirical investigation of its effects on treatment frequency and salmon lice abundance has so far been lacking from scientific literature. We analyze publicly available data on salmon lice abundance and treatment frequency at individual aquaculture sites complemented with information on the use of optical delousing for the entire Norwegian coast in 2023. Our results show that sites relying on optical delousing exhibit a 50 % lower weekly treatment probability. Similar differences applied for all treatment categories and persisted despite a reduced reliance on cleaner fish and in the absence of any indication of a concomitant increase in salmon lice abundance at these sites.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742910"},"PeriodicalIF":3.9,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant gonadotropin induced out-of-season spawning and larval culture of flathead grey mullet (Mugil cephalus)","authors":"Giancarlo Cerrud-Barría ,&nbsp;Alicia Estévez ,&nbsp;Sandra Ramos-Júdez ,&nbsp;Ignacio Giménez ,&nbsp;Neil Duncan","doi":"10.1016/j.aquaculture.2025.742908","DOIUrl":"10.1016/j.aquaculture.2025.742908","url":null,"abstract":"<div><div>The flathead grey mullet (<em>Mugil cephalus</em>) held in intensive captive condition does not sexually mature. The present study, aimed to apply recombinant gonadotropin (rGth) protocols to advance spawning by three months and demonstrate the protocols potential for juvenile production. Eight females and seven males were divided into an rGth group that was treated with species specific recombinant follicle stimulating hormone and recombinant luteinizing hormone and a control group treated with saline. The rGths-treated males completed spermiation and the females completed vitellogenesis and spawning in June–July, three months before the natural spawning period (September – October). Control females did not advance with oogenesis and control males did not advance to spermiation. Eggs were incubated, and hatched larvae were reared in three replicated densities (16, 32, and 51 larvae/L, 100 L tanks) and in grey and black 500 L tanks at 30 larvae/L. Final survival at 16 larvae/L was 14.13 ± 5.2 % at 42 days post- hatching (dph) and a correlation between survival and stocking density predicted 3 juveniles/L could be obtained at 42 dph with an initial density of 38 larvae/L. Larvae reared in black tanks had a superior growth rate (16.70 ± 0.05 mm) in comparison to the grey tanks (11.40 ± 2.80 mm) at 42 dph (<em>P</em> <em>= 0.01</em>). Together, these findings confirm the high efficacy (close to 100 %) of rGths induction protocols, that out-of-season spawning can be induced, and that rGth induced mullet spawning has good potential as a bases for juvenile production.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742908"},"PeriodicalIF":3.9,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced T-cell activation and effector functions contribute to disease resistance in Streptococcus agalactiae-resistant tilapia","authors":"Ming Geng ,&nbsp;Jingfeng Ding ,&nbsp;Jun Xiao ,&nbsp;Liting Chen ,&nbsp;Jiansong Zhang ,&nbsp;Kang Li ,&nbsp;Jie Cheng ,&nbsp;Yi Cao ,&nbsp;Jialong Yang ,&nbsp;Xiumei Wei","doi":"10.1016/j.aquaculture.2025.742903","DOIUrl":"10.1016/j.aquaculture.2025.742903","url":null,"abstract":"<div><div>To address the considerable challenges posed by frequent <em>Streptococcus agalactiae</em> infections in tilapia aquaculture, we previously developed a strain of tilapia with enhanced resistance to this pathogen. Understanding the immune mechanisms behind this resistance is essential for advancing selective breeding strategies. This study concentrates on T-cell activation and effector function to explore the reasons for the more robust immunity in <em>S. agalactiae</em>-resistant tilapia. Through RNA-seq and immunological assays, we demonstrated that resistant tilapia possessed a higher proportion of T cells and enhanced T-cell effector functions compared to susceptible tilapia. Upon <em>in vitro</em> stimulation with anti-tilapia CD3ε plus CD28 monoclonal antibodies (mAbs), or <em>in vivo</em> infection with <em>S. agalactiae</em>, spleen leukocytes from resistant tilapia showed significantly enhanced degrees of Erk1/2 and NF-κB p65 phosphorylation, which are classical signaling pathway of T-cell activation. Once activated, spleen lymphocytes from resistant tilapia expressed higher levels of inflammatory cytokines, such as TNF-α, IL-2, as well as cytotoxic molecule perforin A. Moreover, CD4–1⁺ T cells in the resistant tilapia produced greater amounts of interferon (IFN)-γ, suggesting their superior T-cell effector functions. These findings collectively illustrate how enhanced T-cell activation and effector responses facilitate the optimized immune defenses of resistant tilapia. By shedding light on these mechanisms, our findings provide a valuable foundation for refining breeding programs aimed at increasing disease resistance in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742903"},"PeriodicalIF":3.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative aquaculture performance of genetically improved triploid oysters of ‘Haida No. 3’ line of the Pacific oyster Crassostrea gigas","authors":"Jianmin Zhou ,&nbsp;Chengxun Xu ,&nbsp;Haijun Liu ,&nbsp;Qi Li","doi":"10.1016/j.aquaculture.2025.742905","DOIUrl":"10.1016/j.aquaculture.2025.742905","url":null,"abstract":"<div><div>In recent decades, the oyster industry has shifted from framing primarily diploid oysters to farming almost exclusively triploids in many regions. This shift has been a major driver of the importance of genetic improvement in triploid performance. Tetraploid oysters play a crucial role in triploid production, contributing two-thirds of the chromosome set to their triploid offspring. Recent selective breeding programs focusing on tetraploid oysters have shown promising results, particularly in improving growth rates. However, it remains unclear how successive generations of tetraploid paternal parents affect the aquaculture performance of their triploid progeny. To address this, four mating combinations of the triploid ‘Haida No. 3’ line were established: HTR3 (diploids ♀ × the third-generation tetraploids ♂), HTR2 (diploids ♀ × the second-generation tetraploids ♂), HTR1 (diploids ♀ × the first-generation tetraploids ♂), HTR0 (diploids ♀ × induced tetraploids ♂). The results showed that triploid oysters derived from higher-generation tetraploid parents exhibited significant improvements in shell height, wet weight, and yield at two farming locations (Rongcheng and Huangdao). On day 480, the selective advantage (SA) for shell height was consistently positive, with values ranging from 9.98 % to 27.49 % across both locations. Additionally, the yield of HTR3 exceeded that of the control group (HTR0) by almost 50 %, indicating a significantly increased production at both locations. Triploidy rates were consistently above 95 % across all groups throughout the life stage, increasing with generations of tetraploid parents. However, the percentage of female β (individuals with inactive gametogenesis) decreased and then increased across successive generations of the tetraploid parents at both sites. Overall, this study demonstrated the potential of selective breeding in tetraploids to sustainably improve the aquaculture production of triploid progeny. It also provided valuable insights for optimizing polyploid breeding programs in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742905"},"PeriodicalIF":3.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide methylation and transcriptome analysis in koi carp (Cyprinus carpio var. koi): Uncovering novel molecular mechanisms in skin color differentiation and pigmentation","authors":"Xiulan Shi ,&nbsp;Xiaoyu Chen ,&nbsp;Wenbin Zhu ,&nbsp;Jun Guo ,&nbsp;Kai Lin ,&nbsp;Jianjun Fu ,&nbsp;Lanmei Wang ,&nbsp;Mingkun Luo ,&nbsp;Zaijie Dong","doi":"10.1016/j.aquaculture.2025.742902","DOIUrl":"10.1016/j.aquaculture.2025.742902","url":null,"abstract":"<div><div><em>Koi</em> carp (<em>Cyprinus carpio</em> var. <em>koi</em>) are popular ornamental fish, and their complex mottling and patterning are ideal models for studying the regulatory mechanisms of pigmentation. However, there are few studies addressing the influence of epigenetics, especially DNA methylation on <em>koi</em> coloration and phenotype formation. In this study, we focused on three phenotypes of <em>Kohaku</em> offspring, namely the Rs group (all red-skinned), the Rw group (red-white-skinned) and the Ws group (all white-skinned), each of which underwent DNA methylation and transcriptome sequencing. A comprehensive analysis revealed that the genes in the differentially methylated regions and the differentially expressed genes (DEGs) were significantly enriched in pathways such as retinol metabolism and melanogenesis. The highest number of DEGs was observed in the Rs and Rw groups, while key genes involved in color differentiation, such as <em>mitf, gch</em>2 and <em>bco</em>1, were hypermethylated and regulated in the Rs group. <em>Vivo</em> experiments also showed that inhibition of methylation led to a significant increase in the expression of genes related to the retinol and pteridine pathway (e.g. <em>pnp</em>4<em>a</em>, <em>gch</em>2 and <em>bco</em>2) in the dorsal skin but not in the ventral skin. The results of <em>in situ</em> hybridization of carotenoid metabolism genes (<em>bco</em>1 and <em>bco</em>2) also suggest that DNA methylation is involved in the regulation of different color phenotypes and coloration in various parts of the body. These findings could deepen the understanding of the dynamic changes in DNA methylation that are crucial for the differentiation and variation of skin color in <em>koi</em>, thus revealing the potential influence of epigenetics on pigmentation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742902"},"PeriodicalIF":3.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of short-term storage on sperm quality and microbiome dynamics in blue catfish, Ictalurus furcatus","authors":"Samitha S.N. Liyanage ,&nbsp;Kasun A. Bandara ,&nbsp;Kaylan A. Martin ,&nbsp;Kyle R. Wood ,&nbsp;Jason W. Abernathy ,&nbsp;Benjamin H. Beck ,&nbsp;Timothy J. Bruce ,&nbsp;Luke A. Roy ,&nbsp;Rex A. Dunham ,&nbsp;Xu Wang ,&nbsp;Ian A.E. Butts","doi":"10.1016/j.aquaculture.2025.742883","DOIUrl":"10.1016/j.aquaculture.2025.742883","url":null,"abstract":"<div><div>Supplying high-quality sperm from blue catfish, <em>Ictalurus furcatus</em> is a requirement for channel catfish, <em>I. punctatus</em> ♀ × blue catfish ♂ hybrid production. Storing sperm at low temperatures (4 °C) is a simple and inexpensive method to facilitate hatchery production. Our study objective was to determine the effect of short-term storage on sperm quality and microbiome dynamics in blue catfish. Mature males (<em>n</em> = 8) were selected, testes dissected, milt extracted and stored at 4 °C. Sperm swimming kinematics [motility, curvilinear velocity (VCL), average path velocity (VAP), straight-line velocity (VSL)], viability, and lipid peroxidation were quantified in conjunction with microbial community composition at 0, 24, 48, and 72 h post-storage. Sperm kinematics and viability declined during short-term storage, while malondialdehyde (MDA), an indicator of lipid peroxidation, significantly increased within the first 24 h. Time-specific microbiome changes were observed in sperm during short-term storage. Amplicon sequence variant (ASV) richness was significantly decreased at 72 h. Evenness and Shannon index of bacterial communities significantly decreased at 24 h and remained unchanged from 48 to 72 h. The ASVs belonging to potentially harmful bacterial species, <em>Pseudomonas fluorescens</em> and <em>Aeromonas hydrophila</em>, showed significantly higher abundances following 48 and 72 h. Our results demonstrate changes in sperm quality and microbial communities during short-term storage. These findings can be used to improve sperm storage techniques for hybrid catfish hatchery facilities.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742883"},"PeriodicalIF":3.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Hermetia illucens and Tenebrio molitor meals in diets for European seabass: Effects on growth, nutrient utilisation, intestinal morphology and muscle quality","authors":"Rafaela S. Costa ,&nbsp;Ana Basto ,&nbsp;Marta Monteiro ,&nbsp;Bia Pinho ,&nbsp;Tiago Sá ,&nbsp;Marisa V. Santos ,&nbsp;Daniel Murta ,&nbsp;Johan W. Schrama ,&nbsp;Luisa M.P. Valente","doi":"10.1016/j.aquaculture.2025.742899","DOIUrl":"10.1016/j.aquaculture.2025.742899","url":null,"abstract":"<div><div>This study explored the potential of an insect meal (IM) mixture of black soldier fly larvae (BSFL) and yellow mealworms (YMs) to substitute 3% (diet IM3), 25% (diet IM25) and 50% (diet IM50) of the fishmeal (FM) protein in a control diet (CTRL; 15% FM) for European seabass (<em>Dicentrarchus labrax</em>) juveniles. The four diets were isoproteic and isolipidic and were tested in triplicate in a recirculating aquaculture system with water at 22 °C and 35 ppt salinity, under a 12 h light/12 h dark photoperiod. After a 75-day feeding period, the fish growth performance, nutrient utilisation, intestinal morphology, muscle fatty acid profile and antioxidant capacity were evaluated. The diets containing IM ensured similar growth (DGI = 2.0) and feed efficiency (FCR = 1.0–1.1) to CTRL. Moreover, they promoted comparable nitrogen and energy retention efficiencies (38–40% and 44–47%). However, fish fed with the IM diets presented 20–27% lower faecal phosphorus losses than the control group. Anterior intestine integrity was maintained in all fish, but those fed IM50 displayed longer <em>villi</em> than the control. The muscle fatty acid profile of IM-fed fish also resembled that of the control, with 364–405 mg EPA + DHA per 100 g of fillet. IM25 and IM50 promoted higher lauric acid deposition in the muscle, and the muscle of fish fed with IM50 presented the highest oxygen radical absorbance capacity, suggesting higher antioxidant capacity. Overall, the study shows that the IM mixture can be an adequate protein source for seabass juveniles, also offering functional benefits.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742899"},"PeriodicalIF":3.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses of Piaractus brachypomus (Cuvier, 1818) to anesthesia with linalool and thymol chemotypes of the essential oil of Thymus vulgaris","authors":"Sidney dos Santos Silva ,&nbsp;Imaculada de Morais Carvalho Ananias ,&nbsp;Andre Lima Ferreira ,&nbsp;Thamara Bentivole Magalhães ,&nbsp;Wagner Junio de Freitas Martins ,&nbsp;André de Sena Souza ,&nbsp;Gisele Cristina Favero ,&nbsp;Tulio Pacheco Boaventura ,&nbsp;Ronald Kennedy Luz","doi":"10.1016/j.aquaculture.2025.742896","DOIUrl":"10.1016/j.aquaculture.2025.742896","url":null,"abstract":"<div><div>Two experiments were performed to investigate the anesthetic and physiological responses of <em>Piaractus brachypomus</em> exposed to linalool (EOTV-L) and thymol (EOTV-T) chemotypes of the essential oil of <em>Thymus vulgaris</em> (EOTV). Experiment 1 evaluated induction and recovery times of 110 juvenile fish exposed to EOTV-L (0–300 mg L⁻¹) and EOTV-T (0–200 mg L⁻¹). Experiment 2 evaluated blood collected from 120 juvenile fish immediately after exposure (IAE) and 1 h (1hAE) and 24 h (24hAE) after exposure to a control (ethanol) or to 50 or 100 mg L⁻¹ of each chemotype. EOTV-L showed a linear response plateau effect for induction (<em>P</em> &lt; 0.05), with the inflection at 120.81 mg L⁻¹, and a linear effect for recovery between 94 and 385 s. EOTV-T followed a similar model for induction (inflection at 103.44 mg L⁻¹) and had a quadratic effect for recovery (minimum at 91.20 mg L⁻¹, 151 s). For EOTV- L, hematocrit was higher for the control and 100 mg L⁻¹. Glucose was decreased at 1hAE for 50 and 100 mg L⁻¹ and increased at 24hAE for 50 mg L⁻¹. Plasma protein, hemoglobin and cholesterol showed no differences. For EOTV-T, hematocrit was decreased at 24hAE and triglycerides were increased at 1hAE and decreased at 24hAE. Glucose remained stable at IAE and 1hAE for 50 and 100 mg L⁻¹. Cholesterol increased at 24hAE for the control and 50 and 100 mg L⁻¹ after 1hAE and 24hAE. Both chemotypes ensured adequate induction and recovery times at a concentration of 100 mg L⁻¹, but EOTV-L providing greater metabolic stability and less stress.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742896"},"PeriodicalIF":3.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Joint forecasting of salmon lice and treatment interventions in aquaculture operations","authors":"Benjamin S. Narum,&nbsp;Geir D. Berentsen","doi":"10.1016/j.aquaculture.2025.742849","DOIUrl":"10.1016/j.aquaculture.2025.742849","url":null,"abstract":"<div><div>The need for joint forecasting of parasitic lice and associated preventative treatments stems from large monetary losses and reduced fish welfare associated with such treatments, and the distribution of potential future treatments can be used in operational planning to hedge their associated risk. We present a spatio-temporal forecasting model that accounts for the joint dynamics between lice and treatments where spatial interaction between sites is derived from hydrodynamic transportation patterns. The model-derived forecasting distributions exhibit large heterogeneity between sites at significant levels of exposure, which suggests the forecasting model can provide great value in assisting operational risk management. For the data used in this study, within a 20-week forecast period, our results suggest up to a 40% lice-induced loss in the value of farmed salmon, with differences up to 20%pt. in the average exposure of each site.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742849"},"PeriodicalIF":3.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of P-AKK on glucose and lipid metabolism and immunity of Micropterus salmoides induced by high starch diet","authors":"Guokun Yang ,&nbsp;Boya Yang ,&nbsp;Tianhao Tan ,&nbsp;Mingyue Yin ,&nbsp;Jianing Gu ,&nbsp;Ying Zhang ,&nbsp;Yanmin Zhang ,&nbsp;Xindang Zhang ,&nbsp;Xulu Chang ,&nbsp;Shikun Feng ,&nbsp;Xiaolin Meng","doi":"10.1016/j.aquaculture.2025.742898","DOIUrl":"10.1016/j.aquaculture.2025.742898","url":null,"abstract":"<div><div>As the cheapest energy substance, the carbohydrate exerts a protein-sparing effect in the fish feed. However, excessive dietary carbohydrate intake induces glucose and lipid metabolic dysregulation, intestinal inflammatory responses in teleost. The AKK has been reported that it takes part in regulating glucose and lipid metabolism, inflammatory responses in mammals. However, roles of AKK in teleost remains limited and requires further systematic investigation. In present study, largemouth bass (<em>Micropterus salmoides</em>) was selected as experimental model organism. Following an 8-week culture experiment, the condition factor, triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and glycosylated hemoglobin (GHb) were promoted, while high-density lipoprotein cholesterol (HDL<img>C) was lowered in high starch diet group. Moreover, lipid droplet and glycogen in liver were promoted in high starch diet group. However, the liver fat and glycogen and serum lipid were reduced in the P-AKK supplemental group. In the liver, the mRNA of <em>g-6-pase</em>, <em>fas</em>, and <em>acc-a</em> were significantly inhibited, while the levels of <em>pk</em> and <em>lpl</em> were markedly promoted in P-AKK supplemental group compared with high starch diet and control groups. In the intestinal tissue, the levels of <em>tnf-α</em>, <em>MyD88</em>, <em>il-8</em>, <em>caspase3</em> and <em>caspase9</em> were observably inhibited with P-AKK supplemented into diet. Moreover, the TGF-β and IL-10 protein levels were observably elevated, and the TNF-α protein level was dramatically reduced in P-AKK supplemental group compared with high starch diet and control groups. These results demonstrate that P-AKK plays the crucial part in regulating glucose and lipid metabolism and intestinal inflammatory response in largemouth bass.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742898"},"PeriodicalIF":3.9,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}