Molecular identification and population genetic analysis of a three-way cross hybrid abalone

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-15 DOI:10.1016/j.aquaculture.2025.743189

Haichen Zhang , Qizhen Xiao , Weihong Lin , Zekun Huang , Benben Miao , Yang Gan , Chengyun Li , Caihuan Ke , Xuan Luo , Weiwei You

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引用次数: 0

Abstract

Abalone is economically important shellfish with a worldwide distribution. China is the largest abalone producing and consuming country in the world. In China, interspecific hybrids are used in large-scale abalone production to improve growth performance and stress tolerance. Previous research has shown that the interspecific three-way crosses hybrid abalone (H. gigantea♀ × (Haliotis discus hannai♀ × H. fulgens♂) ♂, SS × DF)display heterosis for growth performance and thermal and hypoxia tolerance. However, systematic analyses of their genetic structure are still lacking. In this study, the population genetic structure of SS × DF and its parents were analysed using genome-wide SNPs. At the same time, specific SNPs of SS × DF were identified. The results showed that SS × DF populations were genetically closer to SS and exhibited intermediate levels of heterozygosity. A population-specific SNP locus was identified, and developed a PCR-based marker that achieved 100 % accuracy in distinguishing SS × DF from other populations. The genomic regions with high differentiation (zFst) were identified, and found that genes enriched in MAPK and Rho GTPase signaling pathways may contribute to the hybrid's superior tolerance to heat and hypoxia. These findings provide molecular evidence of successful three-way hybridization and offer valuable insights for breeding strategies and germplasm management in abalone aquaculture.

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三交杂交鲍鱼的分子鉴定及群体遗传分析

鲍鱼是一种经济上重要的贝类，分布在世界各地。中国是世界上最大的鲍鱼生产国和消费国。在中国，种间杂交被用于大规模鲍鱼生产，以提高生长性能和抗逆性。已有研究表明，种间三交杂交鲍鱼（H. gigantea♀× （Haliotis discus hannai♀× H. fulgens♂）♂，SS × DF）在生长性能、耐热性和耐缺氧性方面均表现出杂种优势。然而，对其遗传结构的系统分析仍然缺乏。本研究利用全基因组snp分析了SS × DF及其亲本的群体遗传结构。同时，鉴定了SS × DF的特异性snp。结果表明，SS × DF群体在遗传上接近SS，表现出中等水平的杂合性。鉴定了一个群体特异性SNP位点，并开发了一个基于pcr的标记，该标记在区分SS × DF与其他群体中达到100%的准确性。鉴定了高分化基因组区（zFst），发现MAPK和Rho GTPase信号通路中富集的基因可能是杂种耐热、耐缺氧的重要原因。这些发现为鲍鱼成功的三向杂交提供了分子证据，并为鲍鱼养殖的育种策略和种质资源管理提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.