Aquaculture最新文献

{"title":"High residual additive genetic variability in a Pacific blue shrimp Litopenaeus stylirostris population after 36 generations of domestication from only 4 estimated founders","authors":"P. Haffray ,&nbsp;F. Enez ,&nbsp;H. Mahunon ,&nbsp;B. Lorgeoux ,&nbsp;P.P. Blanc ,&nbsp;L. Genestout ,&nbsp;A. Rivaton ,&nbsp;J. Bugeon ,&nbsp;C. Liautard-Haag ,&nbsp;P.-A. Gagnaire ,&nbsp;M. Vandeputte","doi":"10.1016/j.aquaculture.2025.742835","DOIUrl":"10.1016/j.aquaculture.2025.742835","url":null,"abstract":"<div><div>Some cultured shrimp populations have been domesticated from a very small number of founders, which likely limits their genetic variability and heightens inbreeding. The population of Pacific blue shrimp <em>Litopenaeus stylirostris</em> in New Caledonia is one such case. It has undergone domestication since 1980 (36 generations at the time of this study) but was based on only four initial founders. Polymorphic SNPs for pedigree assignment and assessment of genetic variability were identified by partial RAD sequencing. The low genetic variability of this domesticated population compared with a wild Mexican population of reference was confirmed with 820 SNP markers.</div><div>To estimate genetic parameters, full-sib and half-sib families (<em>n</em> = 148) were bred from 74 dams and 92 sires using a double-sire insemination of each dam with spermatophores from two sires, the first time that this method has been used in shrimp genetics. At 6 months of age (14.5 g), 1200 progenies were then phenotyped for body weight and external color by digital vision, before and after cooking, using the CIE L*a*b* color space international reference system. This allowed 99.4 % of the progenies were to be successfully assigned to parents with 171 SNP markers.</div><div>Heritability was high for body weight and color traits before and after cooking (h² = 0.41–0.59). Correlations between the blue color (b*) of uncooked shrimps and uncooked body weight was negative but did not differ from zero (r_g =−0.25 ± 0.24). The blue (b*) value of uncooked shrimps was positively correlated with the red (a*) value of cooked shrimps (r_g = 0.48 ± 0.14).</div><div>All results are favorable to pursuing a selective breeding program for blue shrimp in New Caledonia using this population despite its limited number of initial founders and more than 36 generations of domestication. Genetic factors potentially contributing to the results are discussed.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742835"},"PeriodicalIF":3.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suitable length of shrimp meat food sticks for rearing juvenile bigfin reef squid (Sepioteuthis lessoniana)","authors":"Natthawut Chanlek ,&nbsp;Jirapan Satjarak ,&nbsp;Karun Thongprajukaew ,&nbsp;Yuzuru Ikeda ,&nbsp;Nutt Nuntapong","doi":"10.1016/j.aquaculture.2025.742840","DOIUrl":"10.1016/j.aquaculture.2025.742840","url":null,"abstract":"<div><div>The bigfin reef squid (<em>Sepioteuthis lessoniana</em>) is a valued species in Thailand that is now cultivated and has great commercial potential. Nevertheless, the correct feed management for cultivation is still debated, particularly at the juvenile stage and for non-living food items. In the present study, shrimp meat sticks (1.5 to 2.0 mm diameter) of various lengths (4, 5, 6, 7, and 8 mm) were given to juveniles and the effects were observed on growth, feed utilization, morphometrics, digestive enzyme activities, and mantle quality. A completely randomized experimental design was formulated, comprising triplicate groups of 10-day-old juvenile squid (<em>n</em> = 8 per tank) that were given food sticks made solely of whiteleg shrimp (<em>Litopenaeus vannamei</em>) meat for two weeks. At the end of the feeding trial, squid that received 4-mm food sticks, compared with squid that received food sticks of other lengths (<em>p</em> &lt; 0.05), showed significantly higher final body weight, weight gain, and protein efficiency ratio, with a significantly decreased feed conversion ratio. Squids fed 5-mm food sticks grew the same as those fed 4 mm, but their protein efficiency ratio was lower. Longer food lengths (6, 7, and 8 mm) had detrimental effects on squid. The morphometric parameters of head length, head width, dorsal mantle width, fin length, and fin width were significantly higher in squid that received 4-mm food sticks, followed by squid that received 5-mm food sticks. Calorimetric analysis of squid mantle tissue showed that the muscles of squid that ingested longer food sticks developed more myosin and actin, which could assist muscle contraction. These changes were consistent with the observed increased specific activities of the proteolytic enzymes chymotrypsin and trypsin. However, across all dietary groups, there were no differences in RNA concentrations and RNA/protein ratios in muscle tissue, although the mantle protein concentration decreased in squid receiving longer food sticks. These findings imply that developing an artificial feed with a length of 4 mm could enhance the growth and feed efficiency of juvenile bigfin reef squid.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742840"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of MAPK cascade genes in mollusks and their expression patterns in noble scallop Chlamys nobilis under low temperature stress","authors":"Changping Xu ,&nbsp;Huiqi Li ,&nbsp;Meizhen Chen ,&nbsp;Naiqian Zhao ,&nbsp;Beiyu Yan ,&nbsp;Yaoting Liu ,&nbsp;Hongkuan Zhang ,&nbsp;Huaiping Zheng","doi":"10.1016/j.aquaculture.2025.742830","DOIUrl":"10.1016/j.aquaculture.2025.742830","url":null,"abstract":"<div><div>MAPK cascade proteins, including MAPK, MAPK kinases (MAPKK), and MAPKK kinases (MAPKKK) are evolutionarily conserved and exhibit diverse functions, such as energy metabolism, embryonic development, cell differentiation, apoptosis, and responses to external stress. To elucidate the evolutionary relationships of the mitogen-activated protein kinase (MAPK) cascade genes in mollusks, this study selected 54 mollusk genomes as references for systematic screening and comparison of MAPK cascade genes. The results revealed significant variation in gene numbers between Gastropoda and Cephalopoda compared to Bivalvia, with a particularly notable expansion in the <em>MAPKKK</em> gene family. Taking the noble scallop (<em>Chlamys nobilis</em>) from the Bivalvia as an example, we conducted a detailed analysis of the protein structure and conserved motifs of the MAPK cascade genes, indicating a high degree of sequence variability in the <em>MAPKKK</em> genes. Furthermore, we explored the expression patterns of the MAPK cascade genes in various tissues and analyzed their expression differences under chronic and acute low-temperature stress, revealing distinct adaptive strategies employed by mollusks in response to low-temperature stress. Overall, this study not only clarifies the evolutionary relationships of the MAPK cascade genes in mollusks but also characterizes their expression patterns under environmental stress, providing new insights into the adaptive mechanisms of mollusks to environmental changes.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742830"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a novel WAP domain-containing protein with antibacterial immunity from sea cucumber Apostichopus japonicus","authors":"Yiwei Zhang ,&nbsp;Yina Shao ,&nbsp;Weikang Liang ,&nbsp;Chenghua Li","doi":"10.1016/j.aquaculture.2025.742831","DOIUrl":"10.1016/j.aquaculture.2025.742831","url":null,"abstract":"<div><div>In various species, Whey acidic protein domain-containing proteins (WD proteins) are important molecules in the innate immune systems. In this study, a novel WD protein (<em>Aj</em>SWD) was cloned from <em>Apostichopus japonicus</em>, its antibacterial function and mechanism were further studied. The <em>Aj</em>SWD protein contained one WAP domain (10–55 aa) and one KAZAL domain (99–147 aa). <em>Aj</em>SWD mRNA was detected in all chosen tissues, with highest expression level found in the body wall and lower expression level in other detected tissues. Then following the challenge of <em>Vibrio splendidus</em>, we found <em>Aj</em>SWD mRNA and protein expression in coelomocytes and body wall tissues increased to significantly high levels. On antibacterial function research, recombinant <em>Aj</em>SWD and its WAP domain (<em>Aj</em>SWD-WAP) both exhibited obvious bacterial agglutination, PAMPs binding activities and antibacterial activities. Then the antibacterial mechanisms of r<em>Aj</em>SWD and r<em>Aj</em>SWD-WAP were identified as disruption of bacterial cell membranes. Furthermore, after <em>V. splendidus</em> challenge, survival rate of juvenile <em>A. japonicus</em> was effectively improved by both r<em>Aj</em>SWD and r<em>Aj</em>SWD-WAP. Additionally, r<em>Aj</em>SWD-WAP exhibited higher antibacterial efficacy compared to r<em>Aj</em>SWD. For conclusion, this study suggested that r<em>Aj</em>SWD and r<em>Aj</em>SWD-WAP possessed significant antibacterial properties, and the <em>Aj</em>SWD gene plays an important role in the innate immunity of sea cucumber <em>A. japonicus</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742831"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predish: a decision support tool for estimating risk of fish predation in aquaculture systems","authors":"S.A. Lévy ,&nbsp;M. Thomas ,&nbsp;F. Pétronin ,&nbsp;M. Lecomte ,&nbsp;J.M. Mortillaro ,&nbsp;D. Carval ,&nbsp;T. Lecocq","doi":"10.1016/j.aquaculture.2025.742833","DOIUrl":"10.1016/j.aquaculture.2025.742833","url":null,"abstract":"<div><div>The practice of rearing multiple species within the same environment, known as polyculture, is an age-old approach that offers numerous benefits for enhancing the sustainability of aquaculture, provided the species can coexist without detrimental interactions. Predation is one of the most detrimental interactions in polyculture systems and must be avoided to ensure compatibility among species. Since many piscivorous species are commonly reared in aquaculture, it is crucial to avoid species combinations where predation might occur. To address this, we developed a model that estimates predation risk between fish based on their total length in order to avoid empirical trials and thereby uphold ethical standards. This model was calibrated based on niche allometric framework with an extensive dataset on fish predation (4,207 instances) and validated using the specificity, the sensitivity, the True Skill Statistic (TSS) and the area under the curve (AUC) method with a comprehensive dataset of fish predation and non-predation events (13,707 events). The model demonstrated high accuracy with a sensitivity (predation prediction accuracy) of 0.88, specificity (non-predation prediction accuracy) of 0.96, a TSS of 0.84, and an AUC of 0.92. As a practical application, we developed a decision-support tool, Predish, in R software to allow users to estimate predation risk between fish. Predish also includes a database to fill any missing data, making it a valuable resource for designing new polyculture systems that incorporate piscivorous species with minimal predation risk, ultimately supporting aquaculture sustainability.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742833"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of testicular melatonin and cellular stress in an annual spermatogenic cycle of Clarias batrachus","authors":"Akash Acharyya,&nbsp;Kazi Nurul Hasan","doi":"10.1016/j.aquaculture.2025.742842","DOIUrl":"10.1016/j.aquaculture.2025.742842","url":null,"abstract":"<div><div>Melatonin, primarily produced by pineal gland, shows a rhythmic pattern of synthesis in response to environmental cues. Hitherto, the role of testicular melatonin in the regulation of annual spermatogenic cycle is indeterminate in any fish species. Present study aims to explore a correlation, for the first time, of melatonin with cellular stress levels in accordance to the progression of spermatogenesis through six distinct reproductive phases of an annual gonadal cycle of catfish, <em>Clarias batrachus</em> under natural photo-thermal conditions. We assessed gonadosomatic index (GSI), percentage of haploid cells by using DNA intercalating dye in the seminiferous tubules, melatonin concentrations and different intracellular stress parameters in the testis. The concentration of melatonin displayed a distinct seasonal pattern, reaching its peak during the “phase of functional maturity”. Interestingly, the levels of 2′,7′–dichlorofluorescein [reactive oxygen species (ROS) marker], malondialdehyde (MDA) and total nitrate [reactive nitrogen species (RNS)] were found significantly lowered at the verge of “mature state”. Correlation and LOESS (Locally Estimated Scatterplot Smoothing) regression analyses showed a striking positive relationship between testicular melatonin concentration and the activity of antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST). Principal component (PC) analysis also identified the key components of “mature state” viz., GSI, testicular melatonin, haploid cell population (spermatid and spermatozoa), and the activity of enzymatic antioxidants. Cumulatively, melatonin may have a role in growth and maturation of spermatogenic cells by reducing cellular stress to augment the quality of germ cells required for seed production in the culture of any fish species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742842"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of compound probiotics on intestinal and liver transcriptome, immunity and disease resistance of zig-zag eel (Mastacembelus armatus)","authors":"Yuwei Feng,&nbsp;Yiman Chen,&nbsp;Zhiting Zuo,&nbsp;Xianshan Lin,&nbsp;Qiaoyi Chen,&nbsp;Along Gao,&nbsp;Zhide Yu,&nbsp;Chong Han,&nbsp;Hu Shu","doi":"10.1016/j.aquaculture.2025.742836","DOIUrl":"10.1016/j.aquaculture.2025.742836","url":null,"abstract":"<div><div>Probiotics have gained widespread application in aquaculture owing to their high safety profile and lack of antibiotic resistance, thereby enhancing the immune function of fish.</div><div>In this study, zig-zag eels (<em>Mastacembelus armatus</em>) were fed with BC (<em>Bacillus cereus</em> G1-11), EA (<em>Exiguobacterium acetylicum</em> G1-33), MIX (a compound probiotic consisting of G1-11 and G1-33), PC (commercial probiotics) and CON (without probiotics) for 8 weeks, followed by an <em>Aeromonas vickerii</em> challenge. Intestinal transcriptomic analysis revealed significant upregulation of unsaturated fatty acid biosynthesis pathways in the compound probiotic group, alongside enrichment of ABC transporters, galactose metabolism, and C-type lectin receptor signaling pathways. Probiotic supplementation elevated immune-related gene expression in the spleen and enhanced serum antioxidant enzyme activity (superoxide dismutase and catalase) and splenic immunoenzyme levels. After the challenge, the MIX group exhibited the highest survival rate (53.33 %). Histopathological analysis further revealed minimal hepatic and intestinal tissue damage, accompanied by enhanced immune activity in this group. Our findings indicate that the G1-11/G1-33 consortium bolsters innate immunity, oxidative stress resistance, and disease resilience in <em>M. armatus</em>, underscoring its potential as a functional feed additive for sustainable aquaculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742836"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel infectious hematopoietic necrosis virus (IHNV) isolated from white-spotted charr (Salvelinus leucomaenis) is moderately virulent to rainbow trout (Oncorhynchus mykiss)","authors":"Chunyan Guan ,&nbsp;Jingzhuang Zhao ,&nbsp;Yizhi Shao ,&nbsp;Tongyan Lu ,&nbsp;Liming Xu","doi":"10.1016/j.aquaculture.2025.742839","DOIUrl":"10.1016/j.aquaculture.2025.742839","url":null,"abstract":"<div><div>J genogroup infectious hematopoietic necrosis virus (IHNV) is mostly isolated from rainbow trout (<em>Oncorhynchus mykiss</em>). In this study, a novel IHNV strain, designated as H202217, was isolated from diseased white-spotted charr (<em>Salvelinus leucomaenis</em>). The phylogenetic analysis showed that the H202217 strain had the closest relationship with other Chinese J genogroup IHNV strains isolated from rainbow trout. <em>In vitro</em> proliferation experiments showed that the H202217 strain had lower titer but similar immunogenicity with the Sn1203 strain, a reference strain isolated from rainbow trout. In the artificial infection of white- spotted charr, the H202217 strain showed low virulence, and caused a cumulative percentage of mortality (CPM) of 15 %, which was higher than the CPM of 7.5 % caused by the Sn1203 strain. While challenged in rainbow trout, the H202217 strain caused moderate CPM of 22 %, lower than that of 46 % caused by the Sn1203 strain. In addition, the viral loads of the H202217 strain were higher than those of Sn1203 strain in white-spotted charr, while it was the opposite in the rainbow trout. These results suggest that the H202217 strain has better fitness with white-spotted charr and lost partial virulence to rainbow trout compared with the rainbow trout sourced IHNV Sn1203 strain, and which has potential use to reveal the IHNV virulence evolution companied with host jump in the future.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742839"},"PeriodicalIF":3.9,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of l-carnitine supplementation in diet on growth, lipid metabolism, and carnitine biosynthesis in juvenile giant grouper (Epinephelus lanceolatus) fed high plant protein diets","authors":"Yu-Hung Lin ,&nbsp;Yen-Chun Lee ,&nbsp;Chih-Chieh Yeh","doi":"10.1016/j.aquaculture.2025.742821","DOIUrl":"10.1016/j.aquaculture.2025.742821","url":null,"abstract":"<div><div>The present study investigated the effects of supplementing a high plant-protein diet with carnitine on growth performance, body composition, physiological parameters, and lipid metabolism in juvenile giant grouper (<em>Epinephelus lanceolatus</em>). <span>l</span>-carnitine was added to a basal diet with soy protein concentrate (SPC), which replaced 50 % of the fish meal protein, at analyzed carnitine levels of 0.07, 0.18, 0.83, 1.58, and 2.13 g/kg. An all fish meal diet (FM) was used as a reference. Lysine and methionine levels in SPC diets were adjusted to be comparable to those in the reference diet. Triplicate groups of juvenile grouper (initial weight: 19.50 ± 0.03 g) were fed one of six experimental diets and reared in a recirculating aquaculture system for 8 weeks. Weight gain and fish in-fish out ratio were higher (<em>p</em> &lt; 0.05) in fish fed the reference diet than those in fish fed diets containing SPC. Carnitine supplementation in the diet had no significant impact on growth performance, body composition, hepatic oxidative status, or blood biochemical indices. Increasing dietary carnitine levels were associated with a decreasing trend in hepatic gamma-butyrobetaine hydroxylase 1 (BBOX1) gene expression, and an increasing trend in hepatic and muscle carnitine concentrations. This study confirms that giant grouper can endogenously synthesize carnitine via lysine metabolism and BBOX1 activity to meet physiological requirements. Even when fed diets with SPC and deficient in carnitine, juvenile giant grouper do not require additional dietary carnitine supplementation when adequate amounts of methionine and lysine are supplemented.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742821"},"PeriodicalIF":3.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and BSA analysis provide insight into the metabolic regulation mechanism in filefish Thamnaconus septentrionalis under low salt stress","authors":"Jiang Wang ,&nbsp;Wenhao Ren ,&nbsp;Chunyan Zhao ,&nbsp;Li Bian ,&nbsp;Xianliang Meng ,&nbsp;Yichao Ren ,&nbsp;Aihuan Song","doi":"10.1016/j.aquaculture.2025.742792","DOIUrl":"10.1016/j.aquaculture.2025.742792","url":null,"abstract":"<div><div>This study provided insights into the adaption of greenfin horse-faced filefish <em>Thamnaconus septentrionalis</em> toward low-salinity conditions. A combination of antioxidant enzyme activity, transcriptome technology, and BSA sequencing analysis was employed to investigate the osmotic balance, oxidative stress, and immune response in this species. The salinity levels were controlled at 21 PSU (control group), with two treatment groups, Group C (salinity reduction at a rate of 1 PSU per day) and Group A (salinity reduction at a rate of 6 PSU per day), respectively. As a result, with the decrease of salinity, the activities of SOD, CAT, and GSH-PX, as well as the content of MDA, showed varying degrees of increased. The findings indicated that a reduction in salinity can lead to oxidative damage induced by reactive oxygen species (ROS), which in turn activated the activity of antioxidant enzymes in fish to maintain the balance of ROS metabolism. When the salinity was reduced to 15 PSU, there were more DEGs in Groups A and Group C. The KEGG enrichment pathway showed that DEGs were most significantly enriched in the arachidonic acid metabolism, IL-17 signaling, glycine, serine, threonine metabolism, and glutathione metabolism pathways. The BSA sequencing results showed that single nucleotide polymorphisms (SNP) and insertions and deletions (Indel) were co-located on LG08, LG09, LG14, LG15, and LG19 chromosomes. A combined analysis revealed the identification of 52 SNP loci and 22 candidate genes on chromosomes associated with low-salt tolerance trait, which were significantly enriched in tight cell junctions, cytokine receptor interaction pathways, and signal transduction pathways. Upon conducting a joint analysis with the transcriptome, there were five genes, namely <em>CLDN20</em>, <em>IFI44L</em>, <em>MISP</em>, <em>TRIM25</em>, and <em>TSPAN33</em>, that demonstrated significant expression within the transcriptome-filtered DEGs. This study contributes significantly to advancing the understanding of osmotic regulation and immune response mechanism in <em>T. septentrionalis</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742792"},"PeriodicalIF":3.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}