Aquaculture最新文献

{"title":"Single-cell RNA sequencing reveals the immune cell landscape of head kidney in the Perciform fish (Micropterus salmoides)","authors":"Zheng-Yang Zhou ,&nbsp;Chen-Wang Lu ,&nbsp;Zheng-Ran Jian ,&nbsp;Shang-Jie Bai ,&nbsp;Lin-Feng Kuang ,&nbsp;Jin-Lai Gu ,&nbsp;Ming-Zhu Liu ,&nbsp;Peng-Fei Li ,&nbsp;Gao-Xue Wang ,&nbsp;Qing Yu ,&nbsp;Fei Ling ,&nbsp;Jorge Galindo-Villegas ,&nbsp;Tao Liu","doi":"10.1016/j.aquaculture.2025.742918","DOIUrl":"10.1016/j.aquaculture.2025.742918","url":null,"abstract":"<div><div>Teleost fish, a key transitional group in vertebrate evolution, possess immune systems that diverge substantially from those of mammals in structure, composition, and function. Within this group, the order Perciformes stands out for its ecological breadth, evolutionary significance, and value as a model for immunological research. However, the cellular complexity and developmental dynamics of their immune system remain insufficiently characterized. In this study, we employed single-cell RNA sequencing (scRNA-seq) to construct a comprehensive transcriptomic atlas of the head kidney in largemouth bass (<em>Micropterus salmoides</em>), a representative perciform species. We identified and annotated eight major hematopoietic and stromal cell populations, including hematopoietic stem/progenitor cells (HSPCs), erythrocytes (Eryt), granulocytes (Gran), macrophages (Mac), dendritic cells (DC), T and B cells, and mesenchymal stromal cells (MSCs). Subsequent analyses revealed extensive subpopulation heterogeneity across these lineages. We delineated early progenitor subsets, mapped B cell developmental trajectories, and characterized functionally distinct Mac and T cell subtypes. Notably, we identified Eryt cells exhibiting high MHC class II expression, supporting immunological roles beyond oxygen transport. A dot plot of core erythrocyte genes revealed largely lineage-restricted expression. However, several hemoglobin paralogs were also expressed in non-erythroid cells, suggesting functional divergence. Additionally, we identified a putative Gran-erythroid progenitor population and Mac subsets with distinct transcriptional profiles, underscoring the complexity, potential functionality, and wide diversity of specialized immune cell dynamics in Perciformes. Together, these findings significantly advance our understanding of teleost immune cell diversity and lineage specialization. The generated cell atlas provides a valuable resource and novel perspectives for deciphering fish immunity and hematopoiesis, advancing comparative immunology, and informing aquaculture health management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742918"},"PeriodicalIF":3.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population pharmacokinetic modelling of oxytetracycline in rainbow trout (Oncorhynchus mykiss): Effects of route of administration, temperature and ploidy","authors":"Alexis Viel ,&nbsp;Florine Bachelet ,&nbsp;Annaëlle Chailleux ,&nbsp;Céline David ,&nbsp;Lionel Pineau ,&nbsp;Ségolène Calvez ,&nbsp;Antoine Rostang","doi":"10.1016/j.aquaculture.2025.742917","DOIUrl":"10.1016/j.aquaculture.2025.742917","url":null,"abstract":"<div><div>The aim of this study was to perform a pharmacokinetic (PK) study of oxytetracycline (OTC) in rainbow trout using different routes of administration and different doses, and to investigate the influence of the bodyweight, the ploidy status (diploid <em>versus</em> triploid) and the water temperature (10 °C <em>versus</em> 16 °C).</div><div>A cross-over design was used with 361 trout (median bodyweight: 389 g), each fish receiving OTC intravenously (8–30 mg/kg) and orally (50–150 mg/kg), by gavage or by spontaneous intake <em>via</em> a coating of OTC on the feed to mimic farming practice. Each fish, for each modality, was sampled 3–4 times over a period of 11 days, to establish its individual PK profile. Coated feed and plasma samples were assayed using a sensitive HPLC-MS/MS method. A population PK modelling approach was used to analyse plasma data and estimate key PK parameters and their variability.</div><div>A bi-compartmental PK model best described the plasma data. The absolute oral bioavailability appeared to be very low (around 3 %) and the elimination of OTC was slow and strongly influenced by temperature (elimination half-life approximately 79 h at 16 °C <em>versus</em> 113 h at 10 °C). A comparison of spontaneous intake and gavage showed a significant loss of antibiotic due to leaching (about 30 %). Bodyweight and water temperature were the most influential covariates affecting the PK of OTC compared to the effect of ploidy. Pharmacodynamic (PD) studies with OTC against the major trout pathogens are now required to allow a more robust assessment of the expected efficacy thanks to a PK/PD approach.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742917"},"PeriodicalIF":3.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing LMBV infection resistance models in largemouth bass: Exploration and confirmation of pathways and genes relevant to disease resistance in breeding programs","authors":"Shen Li ,&nbsp;Bangsong Meng ,&nbsp;Wenxuan Ouyang ,&nbsp;Liqun Wang ,&nbsp;Jinpeng Chen ,&nbsp;Xin He ,&nbsp;Qiwei Qin ,&nbsp;Min Yang","doi":"10.1016/j.aquaculture.2025.742909","DOIUrl":"10.1016/j.aquaculture.2025.742909","url":null,"abstract":"<div><div>Largemouth bass (<em>Micropterus salmoides</em>) is an important economic freshwater aquaculture species in China, but factors such as disease and a lack of quality seedlings have constrained aquaculture development. In response, we conducted research on breeding for disease resistance and successfully bred a disease-resistant family F1 generation. We used this model to conduct Largemouth bass virus (LMBV) infections to differentiate between resistant and susceptible groups, clarify differences by RNA-sequencing (RNA-seq), and verify disease resistance trait-associated genes using an in vitro infection model. RNA-seq results indicated that pathways such as complement and coagulation cascade, PI3K-Akt, NF-ĸB, and PPAR signaling, and key genes such as C3, PPARα, NFKBIA, JUN, FOS, PI3K, and JAK1 had key roles in LMBV infection resistance. Furthermore, we cloned and preliminarily verified MsPPARα, MsNFKBIA, MsJUN, and MsFOS candidate gene roles in LMBV replication. The results were consistent with RNA-seq data, the low expression of candidate genes effectively inhibited LMBV replication. In conclusion, disease resistance to LMBV is related to lipid metabolism and stabilized inflammatory immune responses. Our study provides a theoretical basis and support for disease-resistance breeding programs for largemouth bass against LMBV infection, and contributes to disease risk and the enrichment of germplasm resources.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742909"},"PeriodicalIF":3.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of temperature on the immune response of WSSV-infected Indian white shrimp (Penaeus indicus): Insights from flow cytometry","authors":"J. Kumaravel,&nbsp;M. Muralidhar,&nbsp;P.K. Patil,&nbsp;K. Vinaya Kumar,&nbsp;J. Ashok Kumar,&nbsp;J. Raymond Jani Angel,&nbsp;Sudheesh K. Prabhudas,&nbsp;K. Karthic,&nbsp;M.S. Shekhar,&nbsp;Akshaya Panigrahi,&nbsp;Kuldeep K. Lal","doi":"10.1016/j.aquaculture.2025.742911","DOIUrl":"10.1016/j.aquaculture.2025.742911","url":null,"abstract":"<div><div>White spot syndrome virus (WSSV) is one of the most virulent pathogens of cultured shrimp. Water temperature is known to have a profound effect on disease expression and viral pathogenesis. This study evaluated the effect of temperature variations on the virus replication and host response. Flow cytometry method was used to analyse <em>Penaeus indicus</em> immune parameters (apoptosis, respiratory burst, cytoplasmic free calcium concentration (cf-Ca²⁺), cell cycle analysis, and phagocytosis) against WSSV infection at 27 °C, 30 °C and 33 °C temperatures at different time points 12hpi, 24 hpi, and 48 hpi. Effect of WSSV replication at different temperatures was quantified by qPCR showing the shrimps exposed to higher temperatures (33 °C) with reduced viral load. At this higher temperature of 33 °C, shrimp responded with elevated percentages of early apoptosis (11.77 ± 2.11 %) and late apoptosis (19.88 ± 4.99), and higher percentage of cf-Ca²⁺ activity (80.10 %) at 48 hpi. Phagocytosis analysis revealed maximum percentage (27 %) of semi granulocytes at 33 °C at 48 hpi. WSSV infected shrimps responded with an increased respiratory burst from 12 hpi to 48 hpi exposed to 33 °C when the virus replication was observed to be low. Decreased percentage of G1 phase in WSSV infected shrimp samples at all time points and temperature, except for minimal increase at 12 hpi at 33 °C indicated cell cycle is deregulated by WSSV infections. The flow cytometric based immune analysis of WSSV infected shrimps in this study suggests elevated temperatures plays a significant role to induce host defense reaction and in inhibiting replication of WSSV. The differentially expressed gene (DEGs) analysis of WSSV infected shrimps at 12, 24 and 48 hpi resulted in 846, 854, 1291 DEGs respectively, offering insights into WSSV infection and shrimp immunity under varying temperature conditions at molecular level.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742911"},"PeriodicalIF":3.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus requirements in sea-cage farmed Atlantic salmon with an emphasis on bone health and digestibility","authors":"Lucia Drábiková ,&nbsp;Saskia Kröckel ,&nbsp;P. Eckhard Witten ,&nbsp;Guido Riesen ,&nbsp;Paul Morris ,&nbsp;Agnés Ostertag ,&nbsp;Martine Cohen-Solal ,&nbsp;Thomas W.K. Fraser ,&nbsp;Per Gunnar Fjelldal","doi":"10.1016/j.aquaculture.2025.742915","DOIUrl":"10.1016/j.aquaculture.2025.742915","url":null,"abstract":"<div><div>Commercial fish feeds are supplemented with highly digestible inorganic phosphorus (P), a limited and expensive resource. As fish excrete excess dietary P, it is necessary to ensure they are fed the correct amounts to reduce costs and the release of phosphorus into the environment. The specific P requirements during the grow-out phase of farmed Atlantic salmon (<em>Salmo salar</em>, L.), which is when feeding intensity is highest, are unknown. In the current study, sea-cage reared salmon under natural light were fed one of six diets with increasing inorganic P levels (6.1-11.2 g/kg total P), from December 2022 (1.8 kg, sampling point I.), through April (2.8 kg, sampling II.), until July 2023 (4.2 kg, sampling III.). Response parameters were P digestibility, P retention, growth, vertebral deformities, vertebral mechanical strength and mineral content, bone microstructure, and microscopic location of bone minerals. Growth of the animals was lower between December-April (0.62 mm/day) with temperatures ranging from 5 to 9 °C compared with the period between April-July (0.98 mm/day, 7-14 °C). Phosphorus digestibility followed a similar trajectory with higher values in the second part of the study. Vertebral deformities were not affected by different dietary P levels. A regular somatic growth, bone mineralisation, and bone mechanical strength were achieved in animals fed 3.7 g/kg available P between December-April and in animals fed 4.6 g/kg available P between April-July. This shows the potential to reduce total dietary P content by 16 –24 % compared to current commercial feeds, without compromising bone mineralisation or skeletal health.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742915"},"PeriodicalIF":3.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Market dynamics and E-commerce satisfaction in China's aquatic food sector: Machine learning and data insights","authors":"Mausam Budhathoki ,&nbsp;Lincen Li ,&nbsp;Hao Xu ,&nbsp;Wenbo Zhang ,&nbsp;Xuzhou Ma ,&nbsp;Sujita Pandey ,&nbsp;Marianne Thomsen","doi":"10.1016/j.aquaculture.2025.742904","DOIUrl":"10.1016/j.aquaculture.2025.742904","url":null,"abstract":"<div><div>China's growing demand for aquatic foods, driven by the rapid expansion of e-commerce, is reshaping global seafood markets and influencing the broader sustainable food system. This transformation is altering domestic market dynamics and creating new challenges and opportunities in international trade, sustainable supply chains, and market competitiveness. This study investigates production trends, import reliance, and consumer e-commerce satisfaction in the aquatic food market, with a focus on implications for sustainable supply chain policy and management. By employing supervised machine learning technique, Support Vector Machines (SVM), along with Latent Dirichlet Allocation (LDA), Mann-Whitney <em>U</em> test, and logistic regression, the study uncovers critical insights into consumer preferences, including the factors driving e-commerce satisfaction, and supply chain dynamics, such as the impact of product freshness and delivery efficiency.</div><div>The study highlights the growing reliance on imports in China's aquatic food market, despite expanding domestic production. Fresh and frozen products, particularly crustaceans and finfish, consistently receive higher consumer satisfaction on e-commerce platforms, primarily due to their quality and freshness. Domestic producers face increasing pressure to meet these high standards, particularly in freshness, quality control, and delivery efficiency. The findings point to challenges in meeting consumer expectations and suggest opportunities for policy interventions to enhance the competitiveness of domestic aquatic food products. The research highlights the transformative role of e-commerce platforms in shaping China's aquatic food market, providing actionable insights for policymakers, producers, and e-commerce stakeholders to navigate emerging trends, improve supply chain efficiency, and meet evolving consumer demands.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742904"},"PeriodicalIF":3.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and application of Bdellovibrio and like organisms (BALOs) for controlling Aeromonas hydrophila in the American eel (Anguilla rostrata) rearing water","authors":"Yanbiao Guo ,&nbsp;Bin Jiang ,&nbsp;Huixiang Chen ,&nbsp;Haifu Zhang ,&nbsp;Jingyin Liu ,&nbsp;Jintao lǚ ,&nbsp;Lingxiao Li ,&nbsp;Wenxi Zhong ,&nbsp;Shichao Wen ,&nbsp;Chunlan Liang ,&nbsp;Yi Huang ,&nbsp;Zhiping Liang ,&nbsp;Junpeng Cai","doi":"10.1016/j.aquaculture.2025.742912","DOIUrl":"10.1016/j.aquaculture.2025.742912","url":null,"abstract":"&lt;div&gt;&lt;div&gt;&lt;em&gt;Bdellovibrio&lt;/em&gt; is a kind of fast-moving bacteria that mainly preys on Gram-negative bacteria. &lt;em&gt;Aeromonas hydrophila&lt;/em&gt; is one of the most common pathogenic bacteria for many animals. However, the potential application of freshwater-derived &lt;em&gt;Bdellovibrio&lt;/em&gt; in protecting eels from &lt;em&gt;A. hydrophila&lt;/em&gt; infection is still unclear. In this study, &lt;em&gt;Klebsiella&lt;/em&gt; was used as the host bacteria, and &lt;em&gt;Bdellovibrio&lt;/em&gt;-and-like organisms (BALOs) were isolated by double-layer plate technique. 32 strains of pathogenic bacteria from different sources including: one Gram-positive pathogenic stain of &lt;em&gt;Bacillus cereus&lt;/em&gt; and 31 Gram-negative pathogenic stains of &lt;em&gt;A. hydrophila, Escherichia coli&lt;/em&gt;, &lt;em&gt;Pantoea agglomerans&lt;/em&gt;, &lt;em&gt;Providencia rettgeri&lt;/em&gt;, &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt;, &lt;em&gt;Salmonella Choleraesuis&lt;/em&gt;, &lt;em&gt;Salmonella Typhimurium&lt;/em&gt;, &lt;em&gt;Serratia odorifera&lt;/em&gt;, &lt;em&gt;Shewanella Putrefaciens&lt;/em&gt;, &lt;em&gt;Vibrio parahaemolyticus&lt;/em&gt;, to name just a few. Moreover, 5 strains of potential probiotics including &lt;em&gt;Bacillus coagulans&lt;/em&gt;, &lt;em&gt;Lactobacillus plantarum&lt;/em&gt;, and &lt;em&gt;Lactobacillus salivarius&lt;/em&gt; were also used to select the &lt;em&gt;Bdellovibrio&lt;/em&gt; strain with a relatively wide lysis spectrum and no adverse effects on potential probiotics. Transmission electron microscopy and BALO-specific polymerase chain reaction [PCR] techniques were employed to identify BALOs strains. The application of BALOs strains in controlling &lt;em&gt;A. hydrophila&lt;/em&gt; infections of American eel (&lt;em&gt;Anguilla rostrata&lt;/em&gt;) was also conducted. Results showed that 8 strains of BALOs, viz. FSBD1, FSBD2, FSBD3, FSBD4, FSBD5, FSBD6, ZZBD1 and ZZBD2 were isolated. Lysis experiments on 32 potentially pathogens showed that FSBD1, FSBD2 FSBD3, FSBD4, FSBD5, FSBD6, ZZBD1 and ZZBD2 lysed 65.6%6 % (21/32), 65.6% (21/32), 65.6 % (21/32), 65.6 %6 % (21/32), 87.5 % (28/32), 75.0 % (24/32), 84.4 % (27/32) and 71.9 % (23/32) pathogens, respectively. Among of them, FSBD5 had the highest lysis rate of 87.5 % (28/32). It showed great lysis ability to 100.0 % (4/4) &lt;em&gt;Salmonella&lt;/em&gt; and 100.0 % (4/4) &lt;em&gt;Aeromonas&lt;/em&gt; tested. However, its lysis rate of potential 5 probiotics strains was 0 % (0/5). Surprisingly, ZZBD1 lysed one Gram-positive pathogenic stain of &lt;em&gt;B. cereus&lt;/em&gt;, with the Gram-positive lysis rate of 16.7 % (1/6). FSBD5 and ZZBD1 were identified as BALOs morphologically by transmission electron microscopy and partial &lt;em&gt;16S rDNA&lt;/em&gt; sequencing. Concerning the strain of FSBD5, compared with &lt;em&gt;A. hydrophila&lt;/em&gt; infection group (AHI), adding the cultured FSBD5 to the rearing water of American eel significantly reduced the number of &lt;em&gt;A. hydrophila&lt;/em&gt; in &lt;em&gt;Bdellovibrio&lt;/em&gt; treatment group (BDT, from 6.19 ± 0.01 log CFU/mL to 2.84 ± 0.08 log CFU/mL, &lt;em&gt;p&lt;/em&gt; &lt; 0.05), and also significantly improved the survival rate of American eel (at the end of 7 days experiment, the survival rate of AHI and BDT group was","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742912"},"PeriodicalIF":3.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of acidification and warming on carbon sequestration capacity in Pacific oysters: Roles of biosynthesis and biodeposition","authors":"Zi-Jing Meng,&nbsp;Cheng-Zhuang Chen,&nbsp;Yu-Qing Ma,&nbsp;Jian-Xue Feng,&nbsp;Ling Liu,&nbsp;Ping Li,&nbsp;Zhi-Hua Li","doi":"10.1016/j.aquaculture.2025.742906","DOIUrl":"10.1016/j.aquaculture.2025.742906","url":null,"abstract":"<div><div>Bivalve aquaculture shows promise as a carbon sink, but its sensitivity to temperature and pH fluctuations highlights the need to study the effects of ocean acidification (OA) and ocean warming (OW) on carbon sequestration. This study investigates the effects of OA and OW on physiological processes and carbon sequestration mediated by biosynthesis and biodeposition in <em>Crassostrea gigas</em>. OA significantly enhances carbon ingestion, reduces respiratory carbon, increases carbon allocation to growth, improves digestive efficiency, and promotes TOC accumulation in soft tissues (all <em>p</em> &lt; 0.05). While OW significantly increases excreted and fecal carbon (p &lt; 0.05), but enhanced digestion compensates for energy loss, sustaining TOC accumulation. Combined OA and OW significantly altered soft tissue carbon sequestration, with values between OA and OW alone (<em>p</em> &lt; 0.05). Notably, their interaction increases biodeposit density and sinking velocity (<em>p</em> &lt; 0.05), potentially enhancing carbon burial. Tissue-specific metabolic responses reveal that muscle tissue prioritizes energy production, whereas the digestive gland follows an opposite trend, resulting in uneven energy distribution. Furthermore, functional predictions based on KEGG pathway analysis and correlation patterns suggest that SCFAs production via tryptophan metabolism might be a potential mechanism through which probiotics modulate host metabolism and contribute to biosynthesis-mediated carbon sequestration. However, disruptions in microbial homeostasis due to an imbalance between probiotics and pathogens in the digestive gland may threaten the long-term sustainability of this sequestration process. These findings provide insights into the complex physiological and microbial responses of oysters under climate change, highlighting potential mechanisms for carbon sequestration in marine ecosystems.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742906"},"PeriodicalIF":3.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms driving temperature-induced early sex change in barramundi (Lates calcarifer)","authors":"Alyssa M. Budd ,&nbsp;Roger Huerlimann ,&nbsp;Jarrod L. Guppy ,&nbsp;Ricardo C.C. Pinto ,&nbsp;Jose A. Domingos ,&nbsp;Dean R. Jerry","doi":"10.1016/j.aquaculture.2025.742882","DOIUrl":"10.1016/j.aquaculture.2025.742882","url":null,"abstract":"<div><div>Sex in fish is remarkably plastic. In some gonochoristic species, temperature exposure during early development can result in epigenetic changes and phenotypic reversal of an otherwise genetically encoded sex. In most of these cases, high temperatures result in an increased proportion of phenotypic males. In sequential hermaphrodites, such as the protandrous barramundi (<em>Lates calcarifer</em>), the effect of temperature on sex is largely unknown. Here, we reared 1-year-old barramundi under four different temperature profiles and examined the effects on mRNA expression, DNA methylation and phenotypic sex. Exposure to high temperature (34 °C) led to early sex change, skewing sex ratios toward transitional and female fish compared to the all-male control group. These phenotypic changes were accompanied by differences in methylation and/or expression of conserved sex-determining genes (e.g., <em>cyp19a1a</em> and <em>dmrt1</em>), and others with putative roles in cellular sensing and signal transduction. Global DNA methylation differences and alternative splicing were observed between sexes but not between temperatures, suggesting that these epigenetic mechanisms occur downstream of putative temperature-modulated signal transduction pathways. The results demonstrate that in contrast to gonochoristic fish, the gonads of sequential hermaphrodites maintain plasticity beyond the completion of sex differentiation, and that exposure to high temperatures leads to an increased proportion of females, rather than males. The findings may assist in the development of temperature as a consumer friendly strategy for sex control in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742882"},"PeriodicalIF":3.9,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing the spatial configuration of scallop suspended cultivation net cages by leveraging seston flux variables based on the lattice Boltzmann hydrodynamics","authors":"Gangqin Tu ,&nbsp;Haifei Liu ,&nbsp;Zhiming Ru ,&nbsp;Tao Sun ,&nbsp;Wei Yang ,&nbsp;Wei Huang ,&nbsp;Li Cheng","doi":"10.1016/j.aquaculture.2025.742863","DOIUrl":"10.1016/j.aquaculture.2025.742863","url":null,"abstract":"<div><div>Suspended scallop farming using cultivation net cages in long-line cultivation structures is a common aquaculture activity. However, cultivation net cages in scallop production lines form obstacles that block currents. The hydrodynamic characteristics of an individual cultivation net cage, as well as the distance between net cages, can influence natural current circulation, further affecting food distribution and solute transport in the scallop farming environment. One of the challenges in scallop farming is to configure farms to optimize total scallop production and individual scallop quality under different environmental conditions. In this study, the appropriate spatial configuration of scallop farms was evaluated through the seston flux distribution characteristics of individual cultivation net cages under different environmental current speeds. A lattice Boltzmann model was established and validated. Then, this model was used to simulate the flow field and seston concentration around a cultivation net cage. The minimum seston flux disturbance of the cultivation net cage is taken as the criterion of the net cage distance. The longitudinal distance of the net cages was determined based on an 80% recovery of seston flux and a transverse distance of less than 5% flux disturbance. The results indicated that the longitudinal distance between net cages decreases with an increasing background flow velocity, and the transverse distance between net cages has little correlation with the background flow velocity. Thus, this study has potential applicability to improve the reforming and planning aquacultural sites.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742863"},"PeriodicalIF":3.9,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}