Aquaculture最新文献

{"title":"Achievement of prosperous nitrification cycle fosters diversity in freshwater microbiome within recirculating aquaculture systems after adjustment period","authors":"Jiho Yang ,&nbsp;Junhyuk Seo ,&nbsp;Jimin Choi ,&nbsp;Yehyeon Cha ,&nbsp;Yunhyeok Jang ,&nbsp;Ju-Ae Hwang ,&nbsp;Seung-Yoon Oh","doi":"10.1016/j.aquaculture.2025.742941","DOIUrl":"10.1016/j.aquaculture.2025.742941","url":null,"abstract":"<div><div>In recirculating aquaculture systems (RAS), a significant challenge involves managing the biological cycling of nitrogenous waste. As a result, there is worldwide focus on the diversity of biological groups involved in nitrogen cycling and their impacts. However, there is still a lack of information concerning the microbial diversity, particularly in relation to biological nitrogen cycling. We hypothesized that biological nitrification in RAS becomes fully established only after a distinct microbial adjustment period following microbial inoculant (MI) treatment, and that this phase features distinct shifts in microbiome composition and nitrogen compound dynamics. To test this, we monitored water chemistry and microbial community structure over an 8-week pre-treatment maturation phase in freshwater RAS for Japanese eel (<em>Anguilla japonica</em>) cultivation. Using 16S rRNA gene amplicon sequencing and qPCR targeting ammonia- and nitrite-oxidizing bacteria, we found that nitrifier abundance increased sharply from week 3 post-treatment. Random forest analysis and structural equation modeling revealed that nitrate levels—serving as a proxy for complete nitrification—had the strongest direct influence on microbiome diversity. Diversity indices and beta-dispersion analyses confirmed that microbial communities during the adjustment period (weeks 0–3) were significantly different from those in the stabilization phase (weeks 4–8). These findings demonstrate the existence of a critical, temporally defined adjustment phase in RAS maturation, during which microbiome structure and nitrification processes co-develop. Our study provides mechanistic insights into microbiome assembly in RAS and highlights the importance of temporal monitoring in optimizing MI-based water treatment strategies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742941"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Market dynamics and E-commerce satisfaction in China's aquatic food sector: Machine learning and data insights","authors":"Mausam Budhathoki ,&nbsp;Lincen Li ,&nbsp;Hao Xu ,&nbsp;Wenbo Zhang ,&nbsp;Xuzhou Ma ,&nbsp;Sujita Pandey ,&nbsp;Marianne Thomsen","doi":"10.1016/j.aquaculture.2025.742904","DOIUrl":"10.1016/j.aquaculture.2025.742904","url":null,"abstract":"<div><div>China's growing demand for aquatic foods, driven by the rapid expansion of e-commerce, is reshaping global seafood markets and influencing the broader sustainable food system. This transformation is altering domestic market dynamics and creating new challenges and opportunities in international trade, sustainable supply chains, and market competitiveness. This study investigates production trends, import reliance, and consumer e-commerce satisfaction in the aquatic food market, with a focus on implications for sustainable supply chain policy and management. By employing supervised machine learning technique, Support Vector Machines (SVM), along with Latent Dirichlet Allocation (LDA), Mann-Whitney <em>U</em> test, and logistic regression, the study uncovers critical insights into consumer preferences, including the factors driving e-commerce satisfaction, and supply chain dynamics, such as the impact of product freshness and delivery efficiency.</div><div>The study highlights the growing reliance on imports in China's aquatic food market, despite expanding domestic production. Fresh and frozen products, particularly crustaceans and finfish, consistently receive higher consumer satisfaction on e-commerce platforms, primarily due to their quality and freshness. Domestic producers face increasing pressure to meet these high standards, particularly in freshness, quality control, and delivery efficiency. The findings point to challenges in meeting consumer expectations and suggest opportunities for policy interventions to enhance the competitiveness of domestic aquatic food products. The research highlights the transformative role of e-commerce platforms in shaping China's aquatic food market, providing actionable insights for policymakers, producers, and e-commerce stakeholders to navigate emerging trends, improve supply chain efficiency, and meet evolving consumer demands.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742904"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of salinity, temperature and photoperiod on egg production of Pseudodiaptomus annandalei (Copepoda: Calanoida) used as live feed in Mariculture","authors":"Neethu Kuriakose ,&nbsp;Imelda Joseph","doi":"10.1016/j.aquaculture.2025.742921","DOIUrl":"10.1016/j.aquaculture.2025.742921","url":null,"abstract":"<div><div>Copepods are essential for maintaining marine ecosystems, and nauplii of <em>Pseudodiaptomus annandalei</em> serves as a crucial food source for fish larvae. This study investigated how salinity, temperature, and photoperiod (Light: Dark cycle) affect the reproductive traits of this copepod in a controlled environment. Clutch size was highest at 15 ppt salinity, with an average of 18.00 ± 0.58 eggs, while it dropped significantly to 9.33 ± 0.33 at 30 ppt. The dimensions of egg sacs and the total length of adult females were also smallest at 30 ppt. Among different salinities, the shortest times to first and total maturity were recorded at 10 ppt, whereas the longest times occurred at 30 ppt. Temperature also played a critical role; clutch size peaked at 28 °C and was lowest at 25 °C, with an average of 11.00 ± 0.41. Photoperiod had a limited impact on reproductive output, but the highest clutch size was observed in a 0 hL:24 hD light cycle. The findings suggest that optimal conditions for maximizing nauplii production in <em>P. annandalei</em> are a salinity of 15 ppt, a temperature of 28 °C, and a continuous dark photoperiod (0 hL:24 hD). Under these conditions, the highest hatching percentage was achieved, highlighting the importance of these factors for effective breeding in this copepod species. This research provides valuable insights for marine ecology and aquaculture, emphasizing the significance of environmental conditions in copepod reproduction.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742921"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the editor: Comment on development of a TGC-based growth model for the olive flounder, Paralichthys olivaceus, and its application in developing a fish growth simulator architecture","authors":"Jianpeng Yu,&nbsp;Baochao Liao","doi":"10.1016/j.aquaculture.2025.742924","DOIUrl":"10.1016/j.aquaculture.2025.742924","url":null,"abstract":"","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742924"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of carotenoid accumulation related genes reveals key genes underlying ovarian color divergence in bivalves: Insights from Chlamys nobilis and Crassostrea gigas","authors":"Huiqi Li ,&nbsp;Changping Xu ,&nbsp;Yifan Li ,&nbsp;Xuanbing Wu ,&nbsp;Meizhen Chen ,&nbsp;Beiyu Yan ,&nbsp;Yaoting Liu ,&nbsp;Taixin Chen ,&nbsp;Caixuan Wang ,&nbsp;Hongkuan Zhang ,&nbsp;Huaiping Zheng","doi":"10.1016/j.aquaculture.2025.742929","DOIUrl":"10.1016/j.aquaculture.2025.742929","url":null,"abstract":"<div><div>Gonadal color polymorphism is a widespread phenomenon in marine species. It has critical implications for health assessment, reproductive signaling, and environmental adaptation. Carotenoids, lipid-soluble pigments with antioxidant and immunomodulatory functions, play central roles in gonadal pigmentation. However, the genetic mechanisms underlying interspecific divergence in gonadal coloration remain poorly understood, particularly in bivalves. In this study, we conducted a comprehensive genomic and transcriptomic analysis of five carotenoid-related gene families—scavenger receptor class B (<em>SR-B</em>), vitellogenin (<em>Vg</em>), glutathione S-transferase P (<em>GSTP</em>), steroidogenic acute regulatory protein (<em>STARD3</em>), and carotenoid cleavage dioxygenase (<em>CCD</em>)-across 18 bivalve species, comprising 3 <em>Mytilidae</em>, 7 <em>Ostreidae</em>, and 8 <em>Pectinidae</em>. Phylogenetic and gene family contraction/expansion analyses revealed significant expansion of <em>CCD</em> genes in scallops and conservation of <em>Vg</em> and <em>SR-B</em> genes across bivalves. Tissue expression analysis showed that <em>Vg</em> and <em>GSTP</em> were predominantly expressed in the ovaries of scallops (<em>Chlamys nobilis</em>), with <em>CnVG2</em> and <em>CnGSTP1</em> exhibiting striking female-biased expression. In contrast to scallops, oysters (<em>Crassostrea gigas</em>) showed limited sexual dimorphism in these genes. Expression analysis of ovarian developmental stage revealed that <em>Vg</em> and <em>GSTP</em> were significantly upregulated during ovarian maturation in scallops, and no significant changes in <em>CCD</em> expression, suggesting a “high uptake, low degradation” model driving carotenoid accumulation in scallops. Collectively, these findings highlight the divergent roles of carotenoid-related genes in scallops and oysters, with scallops utilizing these genes for vivid ovarian pigmentation and oysters repurposing them for stress resilience. This study provides new insights into the genetic basis of gonadal color divergence in bivalves and potential molecular targets for optimizing reproductive strategy in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742929"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing reveals the immune cell landscape of head kidney in the Perciform fish (Micropterus salmoides)","authors":"Zheng-Yang Zhou ,&nbsp;Chen-Wang Lu ,&nbsp;Zheng-Ran Jian ,&nbsp;Shang-Jie Bai ,&nbsp;Lin-Feng Kuang ,&nbsp;Jin-Lai Gu ,&nbsp;Ming-Zhu Liu ,&nbsp;Peng-Fei Li ,&nbsp;Gao-Xue Wang ,&nbsp;Qing Yu ,&nbsp;Fei Ling ,&nbsp;Jorge Galindo-Villegas ,&nbsp;Tao Liu","doi":"10.1016/j.aquaculture.2025.742918","DOIUrl":"10.1016/j.aquaculture.2025.742918","url":null,"abstract":"<div><div>Teleost fish, a key transitional group in vertebrate evolution, possess immune systems that diverge substantially from those of mammals in structure, composition, and function. Within this group, the order Perciformes stands out for its ecological breadth, evolutionary significance, and value as a model for immunological research. However, the cellular complexity and developmental dynamics of their immune system remain insufficiently characterized. In this study, we employed single-cell RNA sequencing (scRNA-seq) to construct a comprehensive transcriptomic atlas of the head kidney in largemouth bass (<em>Micropterus salmoides</em>), a representative perciform species. We identified and annotated eight major hematopoietic and stromal cell populations, including hematopoietic stem/progenitor cells (HSPCs), erythrocytes (Eryt), granulocytes (Gran), macrophages (Mac), dendritic cells (DC), T and B cells, and mesenchymal stromal cells (MSCs). Subsequent analyses revealed extensive subpopulation heterogeneity across these lineages. We delineated early progenitor subsets, mapped B cell developmental trajectories, and characterized functionally distinct Mac and T cell subtypes. Notably, we identified Eryt cells exhibiting high MHC class II expression, supporting immunological roles beyond oxygen transport. A dot plot of core erythrocyte genes revealed largely lineage-restricted expression. However, several hemoglobin paralogs were also expressed in non-erythroid cells, suggesting functional divergence. Additionally, we identified a putative Gran-erythroid progenitor population and Mac subsets with distinct transcriptional profiles, underscoring the complexity, potential functionality, and wide diversity of specialized immune cell dynamics in Perciformes. Together, these findings significantly advance our understanding of teleost immune cell diversity and lineage specialization. The generated cell atlas provides a valuable resource and novel perspectives for deciphering fish immunity and hematopoiesis, advancing comparative immunology, and informing aquaculture health management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742918"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of present heat waves in freshwater environments during out-growth phase in rainbow trout, Oncorhynchus mykiss, underscores the need of new wisely designed mitigation strategies: climatological, physiological and molecular analyses","authors":"Marta F. Riesco ,&nbsp;Laura Calvo-Rodríguez ,&nbsp;Maria Ángeles Esteban ,&nbsp;Paulino de Paz ,&nbsp;Ignacio Fernández","doi":"10.1016/j.aquaculture.2025.742879","DOIUrl":"10.1016/j.aquaculture.2025.742879","url":null,"abstract":"<div><div>Extreme temperature events are urgent concerns for fish farming sustainability. Only scarce studies have focused on the potential effects of heat waves (HW). Here, the incidence of HW in a freshwater basin and the related abiotic conditions (dissolved oxygen content, water flow rate and water level) in 17 different locations of the major rainbow trout (<em>Oncorhynchus mykiss</em>) production region in Spain (Castilla y León), have been explored during the last 4 years. Results showed that, HW duration progressively increased (up to 71 days), mean intensities (I_mean) ranged 0.56–5.47 °C, and maximum intensities (I_max) reached up to 8.94 °C. Based on this, the growth performance and the biochemical and molecular responses at systemic and local levels in rainbow trout specimens during the out-growth phase (approx. 29 cm and 350 g) have been assessed when exposed to a conservative model of single HW lasting 19 days, with an I_mean of 4.18, and an I_max of 6.9 and (HW group), or maintained at 14.5 °C (Control group). Specimens exposed to this HW event in the freshwater showed different responses depending on the parameter and level considered. In exposed specimens, body weight tended to decrease, while malondialdehyde (MDA) content in skin mucus increased. Blood plasma activities of superoxide dismutase and glutathione peroxidase were also increased in HW specimens. Finally, although HW exposure didn't affect the expression of redox balance genes at gills and liver, the expression of particular heat shock related genes was up-regulated. Considering current and future climate change scenarios, higher environmental monitoring effort should be done for wise decision-making on operational activities (e.g., better selection of locations, improved rearing system designs and further adapted husbandry practices, preference for resistant fish strains, and/or implementing better systems for monitoring and weather forecasting) by fish farm managers to cope with HW events. Specifically, climatology data on incidence, intensity and duration of HW in southern Europe should be considered for strategic spatial planning for long-term continental aquaculture sustainability.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742879"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic resonance imaging for rapid, noninvasive and accurate assessment of ovarian maturation in live Channa argus (Cantor, 1842)","authors":"Wenqian Sun ,&nbsp;Weiyin Vivian Liu ,&nbsp;Xingyu Chen ,&nbsp;Guangcan Lin ,&nbsp;Hao Li ,&nbsp;Yanting Song ,&nbsp;Chengdong Peng ,&nbsp;Wen Xiong ,&nbsp;Yu Peng ,&nbsp;Shangong Wu ,&nbsp;Shouqi Xie ,&nbsp;Sovan Lek ,&nbsp;Mette Sørensen ,&nbsp;Haokun Liu ,&nbsp;Dong Liu ,&nbsp;Lei Pan","doi":"10.1016/j.aquaculture.2025.742872","DOIUrl":"10.1016/j.aquaculture.2025.742872","url":null,"abstract":"<div><div>Current methods for the determination of ovarian maturation in fish have shortcomings. They are often time-consuming, inaccurate, and fish are sacrificed during the procedure. A novel noninvasive and accurate method to assess ovarian maturation can be to use magnetic resonance imaging (MRI). Long scanning time is a limitation for use in fish. A clinical MRI with a new sequence employing a shorter scanning time may be more feasible for the assessment of ovarian maturation in female fish. The aim of this study was to investigate ovarian maturation in <em>Channa argus</em> by clinical MRI with the three-dimensional fast spin echo Cube-flex (3D-FSE-Cube-Flex) T₂WI sequence, compared with two traditional sequences. The results demonstrated that compared with two traditional sequences, the 3D-FSE-Cube-Flex T₂WI sequence greatly reduced scanning time (only requiring 140 s per fish) without reducing the scanning quality. A significant correlation (R² = 0.985, <em>p</em> &lt; 0.001, <em>N</em> = 112) was found between the percentage volume of the ovary measured by MRI and the gonadosomatic index (GSI). Besides, ovarian maturation MRI images at different development stages were captured. These findings suggest that clinical MRI with the 3D-FSE-Cube-Flex T₂WI sequence is a rapid, accurate, and noninvasive method for assessing ovarian maturation, with great potential applications in sustainable aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742872"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and application of Bdellovibrio and like organisms (BALOs) for controlling Aeromonas hydrophila in the American eel (Anguilla rostrata) rearing water","authors":"Yanbiao Guo ,&nbsp;Bin Jiang ,&nbsp;Huixiang Chen ,&nbsp;Haifu Zhang ,&nbsp;Jingyin Liu ,&nbsp;Jintao lǚ ,&nbsp;Lingxiao Li ,&nbsp;Wenxi Zhong ,&nbsp;Shichao Wen ,&nbsp;Chunlan Liang ,&nbsp;Yi Huang ,&nbsp;Zhiping Liang ,&nbsp;Junpeng Cai","doi":"10.1016/j.aquaculture.2025.742912","DOIUrl":"10.1016/j.aquaculture.2025.742912","url":null,"abstract":"&lt;div&gt;&lt;div&gt;&lt;em&gt;Bdellovibrio&lt;/em&gt; is a kind of fast-moving bacteria that mainly preys on Gram-negative bacteria. &lt;em&gt;Aeromonas hydrophila&lt;/em&gt; is one of the most common pathogenic bacteria for many animals. However, the potential application of freshwater-derived &lt;em&gt;Bdellovibrio&lt;/em&gt; in protecting eels from &lt;em&gt;A. hydrophila&lt;/em&gt; infection is still unclear. In this study, &lt;em&gt;Klebsiella&lt;/em&gt; was used as the host bacteria, and &lt;em&gt;Bdellovibrio&lt;/em&gt;-and-like organisms (BALOs) were isolated by double-layer plate technique. 32 strains of pathogenic bacteria from different sources including: one Gram-positive pathogenic stain of &lt;em&gt;Bacillus cereus&lt;/em&gt; and 31 Gram-negative pathogenic stains of &lt;em&gt;A. hydrophila, Escherichia coli&lt;/em&gt;, &lt;em&gt;Pantoea agglomerans&lt;/em&gt;, &lt;em&gt;Providencia rettgeri&lt;/em&gt;, &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt;, &lt;em&gt;Salmonella Choleraesuis&lt;/em&gt;, &lt;em&gt;Salmonella Typhimurium&lt;/em&gt;, &lt;em&gt;Serratia odorifera&lt;/em&gt;, &lt;em&gt;Shewanella Putrefaciens&lt;/em&gt;, &lt;em&gt;Vibrio parahaemolyticus&lt;/em&gt;, to name just a few. Moreover, 5 strains of potential probiotics including &lt;em&gt;Bacillus coagulans&lt;/em&gt;, &lt;em&gt;Lactobacillus plantarum&lt;/em&gt;, and &lt;em&gt;Lactobacillus salivarius&lt;/em&gt; were also used to select the &lt;em&gt;Bdellovibrio&lt;/em&gt; strain with a relatively wide lysis spectrum and no adverse effects on potential probiotics. Transmission electron microscopy and BALO-specific polymerase chain reaction [PCR] techniques were employed to identify BALOs strains. The application of BALOs strains in controlling &lt;em&gt;A. hydrophila&lt;/em&gt; infections of American eel (&lt;em&gt;Anguilla rostrata&lt;/em&gt;) was also conducted. Results showed that 8 strains of BALOs, viz. FSBD1, FSBD2, FSBD3, FSBD4, FSBD5, FSBD6, ZZBD1 and ZZBD2 were isolated. Lysis experiments on 32 potentially pathogens showed that FSBD1, FSBD2 FSBD3, FSBD4, FSBD5, FSBD6, ZZBD1 and ZZBD2 lysed 65.6%6 % (21/32), 65.6% (21/32), 65.6 % (21/32), 65.6 %6 % (21/32), 87.5 % (28/32), 75.0 % (24/32), 84.4 % (27/32) and 71.9 % (23/32) pathogens, respectively. Among of them, FSBD5 had the highest lysis rate of 87.5 % (28/32). It showed great lysis ability to 100.0 % (4/4) &lt;em&gt;Salmonella&lt;/em&gt; and 100.0 % (4/4) &lt;em&gt;Aeromonas&lt;/em&gt; tested. However, its lysis rate of potential 5 probiotics strains was 0 % (0/5). Surprisingly, ZZBD1 lysed one Gram-positive pathogenic stain of &lt;em&gt;B. cereus&lt;/em&gt;, with the Gram-positive lysis rate of 16.7 % (1/6). FSBD5 and ZZBD1 were identified as BALOs morphologically by transmission electron microscopy and partial &lt;em&gt;16S rDNA&lt;/em&gt; sequencing. Concerning the strain of FSBD5, compared with &lt;em&gt;A. hydrophila&lt;/em&gt; infection group (AHI), adding the cultured FSBD5 to the rearing water of American eel significantly reduced the number of &lt;em&gt;A. hydrophila&lt;/em&gt; in &lt;em&gt;Bdellovibrio&lt;/em&gt; treatment group (BDT, from 6.19 ± 0.01 log CFU/mL to 2.84 ± 0.08 log CFU/mL, &lt;em&gt;p&lt;/em&gt; &lt; 0.05), and also significantly improved the survival rate of American eel (at the end of 7 days experiment, the survival rate of AHI and BDT group was","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742912"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of CSAD and its genomic variations modulating taurine synthesis and heat adaptation between two congeneric oyster species","authors":"Meiqian Pang ,&nbsp;Haigang Qi ,&nbsp;Chaogang Wang ,&nbsp;Rihao Cong ,&nbsp;Mingyang Du ,&nbsp;Zhuxiang Jiang ,&nbsp;Min Wang ,&nbsp;Li Li ,&nbsp;Guofan Zhang","doi":"10.1016/j.aquaculture.2025.742933","DOIUrl":"10.1016/j.aquaculture.2025.742933","url":null,"abstract":"<div><div>Oysters are characterized by an extremely high taurine content, a feature that constitutes one of the key reasons for the nutritional and commercial value of these shellfish. Nevertheless, within these organisms, the functions of key metabolic genes and their regulatory mechanisms remain poorly understood. In the current study, we confirmed that cysteine sulfinic acid decarboxylase (CSAD) catalyzes taurine synthesis in two congeneric oyster species: the Pacific oyster <em>Crassostrea gigas</em> and the Portuguese oyster <em>Crassostrea angulata</em>. These oysters are notable for their high aquaculture yields and distinct thermal tolerance levels. <em>Csad</em> displayed distinct thermal response patterns in the two oyster species and significantly contributed to the enhancement of thermal tolerance. Three genomic variations in the <em>Csad</em> promoter region were identified. These variations enhanced the transcriptional activity by varying degrees under heat stress conditions, with the activity increasing from 2.52-fold to 3.19-fold, 2.81-fold and 4.30-fold respectively. Our findings establish Poly (ADP-ribose) polymerase 1 (PARP1) as a transcriptional coregulator mediating the species-divergent thermal response of <em>Csad</em>. This research fills a significant void in our understanding of the regulatory mechanisms of taurine metabolism in marine invertebrates and has the potential to lay a theoretical groundwork for the genetic improvement of oysters via molecular-based methodologies.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742933"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}