Aquaculture最新文献

{"title":"Exploring the potential regulation mechanisms of host-microbiome comprehensive interactions on growth traits in Cyprinus carpio","authors":"Li Feng ,&nbsp;Qinglei Xu ,&nbsp;Jianxin Feng ,&nbsp;Yanhui Wang ,&nbsp;Shangqi Li ,&nbsp;Qin Zhang ,&nbsp;Linyan Zhou ,&nbsp;Jian Xu","doi":"10.1016/j.aquaculture.2025.742522","DOIUrl":"10.1016/j.aquaculture.2025.742522","url":null,"abstract":"<div><div>The interaction between gut microbiota and host genetic factors in regulating host growth is receiving increasing attention. However, the mechanism underlying host variations and gut microbiota in regulating growth is still unclear in aquatic species. In this study, muscle and gut microbiota samples were collected from common carp. Host genetic markers and gut microbiota 16S rRNA sequencing data were analyzed to dissect the interactions between host and gut microbiota on growth trait, revealing differences in the overall gut microbiota between the high-growth and low-growth groups. Ten bacterial taxa showed significant differences between the two groups. A genome-wide association study indicated three loci associated with the growth trait, while genes harboring the three loci were found to be associated with <em>Iamia</em> and <em>Thermoactinomyces</em>. The three loci (Chr18:6901682; Chr23:10968715; Chr32:16293406) were located within the genes <em>PARD3B</em>, <em>RCC2</em>, and <em>ITGA10</em>. Mutations at the <em>RCC2</em> locus may disrupt the cell regulatory cycle, leading to an increase in immune-related bacterial taxa, triggering an immune response in fish, and significantly impacting the growth rate of the common carp. Our study offers valuable insights into how host genome variations can influence growth traits by impacting gut microbiota, serving as a reference for understanding the intricate relationship between fish and gut microbiota. This research holds paramount significance for advancing engineered microbiome technology in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742522"},"PeriodicalIF":3.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance in global catfish aquaculture: Trends, control strategies, and public health implications","authors":"Manoj Kumar Shahi ,&nbsp;Justice Opare Odoi ,&nbsp;Saran Anuntawirun ,&nbsp;Nawaphorn Roongrojmongkhon ,&nbsp;Worapong Singchat ,&nbsp;Thitipong Panthum ,&nbsp;Jiraboon Prasanpan ,&nbsp;Darren K. Griffin ,&nbsp;Kornsorn Srikulnath ,&nbsp;Saharuetai Jeamsripong","doi":"10.1016/j.aquaculture.2025.742552","DOIUrl":"10.1016/j.aquaculture.2025.742552","url":null,"abstract":"<div><div>Aquaculture plays a crucial role in meeting global protein needs. Concerns are growing however about antimicrobial resistance (AMR) in aquaculture and its impact on public health and food security. AMR mostly arises due to the widespread use of antimicrobials, poor farming practices, and inadequate biosecurity measures. Factors contributing to AMR include the over- and improper use of antimicrobials in aquaculture, regulatory shortcomings, environmental contamination, horizontal gene transfer, as well as co- and cross-resistance. This review examines the contribution of various global catfish species aquaculture to the increase in AMR. We highlight the widespread occurrence of AMR, focusing on common antimicrobial classes used in aquaculture and human medicine. These include penicillins, tetracyclines, macrolides, and sulfonamides. Certain pathogens, particularly extended-spectrum β-lactamase (ESBL)-producing bacteria, pose a significant public health concern, however, the resistance of catfish to carbapenems remains limited. We review the implications of AMR for sustainability. We also emphasize the importance of understanding these factors to create effective monitoring programs, stewardship strategies and protection against AMR in the One Health paradigm. This study highlights the importance of integration of One Health principles to mitigate AMR in aquaculture by recognizing the interconnectedness of humans, animals, and the environment. To ensure public health, food safety, and the industry's future, policymakers and industry leaders must take decisive action, including stricter antibiotic regulations, regular AMR monitoring, ethical farming practices, and investment in sustainable alternatives.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742552"},"PeriodicalIF":3.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveal the effect of different dietary plant protein sources on the microbiota-gut-digestive gland axis of abalone Haliotis dicus hannai","authors":"Shuoli Ma ,&nbsp;Ping Xiao ,&nbsp;Zhenhua Wu ,&nbsp;Yanlin Guo ,&nbsp;Kangsen Mai ,&nbsp;Wenbing Zhang","doi":"10.1016/j.aquaculture.2025.742501","DOIUrl":"10.1016/j.aquaculture.2025.742501","url":null,"abstract":"<div><div>Current research on the nutritional functions of plant proteins in abalone primarily focuses on fishmeal replacement, while systematic investigation into the differential effects of various plant protein sources under equivalent fishmeal conditions remains scarce. The effect of dehulled soybean meal (DSM), soy protein concentrate (SPC) and corn gluten meal (CGM) on abalone <em>H. discus hannai</em> were evaluated through 16S rRNA, transcriptomics and metabolomics in the present study. The core gut microbiota of abalone varied among the different diet groups (DSM, SPC and GCM): <em>Mycoplasma</em> (57.51 %, 64.39 %, 70.23 %). Multi-omics integrative analysis indicated that abalone fed with the SPC diet effectively activated nutrients metabolism and amino acid biosynthesis via microbiota -gut-digestive gland axis. Abalone fed with the SPC diet had the lowest chewiness, gumminess and hardness of muscle. Moreover, abalone fed with the DSM diet significantly increased the abundance of Bacteroidetes, which significantly impacted the glycolysis/gluconeogenesis metabolism through the microbiota-gut-digestive gland axis. In contrast, the CGM diet led to a significant changes in the gut microbiota of abalone, characterized by an increased abundance of Marinifilaceae and Bacteroides, while the abundance of Proteobacteria decreased. This microbial change was associated with reduced activity of the gut digestive enzyme pectinase, ultimately affecting the efficiency of nutrient absorption. In addition, there was a correlation between gut microbial abundance, gut enzyme activities and cell-free hemolymph metabolite content, which might be the reason for the difference in utilization of different plant protein sources by abalone. These results light the functional interaction between the gut milieu and the digestive gland axis under the efficacy of different protein sources.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742501"},"PeriodicalIF":3.9,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frog virus 3: Prevalence survey and quantitative analysis in American bullfrogs using TaqMan MGB probe real-time fluorescence quantitative PCR","authors":"Hua Li ,&nbsp;Xiaobing Liu ,&nbsp;Jingyang Sun ,&nbsp;Han Lin ,&nbsp;Kesong Chen ,&nbsp;Yueming Hu ,&nbsp;Mengzhen Jiang ,&nbsp;Biao Jiang ,&nbsp;Wei Li ,&nbsp;Youlu Su ,&nbsp;Qing Wang ,&nbsp;Chun Liu","doi":"10.1016/j.aquaculture.2025.742527","DOIUrl":"10.1016/j.aquaculture.2025.742527","url":null,"abstract":"<div><div>The American bullfrog (<em>Aquarana catesbeiana</em>) has emerged as a significant aquaculture species in China. A substantial rise in breeding density can deteriorate its breeding environment, increasing its susceptibility to diseases. Frog Virus 3(FV3) is an important pathogen of the bullfrog that can result in hemorrhage and necrosis of tissues after infection. However, our understanding of the prevalence and pathogenesis of FV3 in farmed bullfrogs remains limited. This study aimed to analyze the changes in viral load within the organs of infected bullfrogs and to investigate the prevalence of FV3 infection across various bullfrog farms, thereby providing insights into the pathogenic mechanisms of FV3. A rapid, specific, and highly sensitive a TaqMan minor groove binder probe fluorescence real-time quantitative polymerase chain reaction (qPCR) assay was developed for detection and quantification of FV3. The established qPCR method was used to study the distribution of FV3 in bullfrog tissues. It was found that FV3 could result in asymptomatic long-term persistence in tissues following infection in bullfrogs, with the liver being the primary site of replication. Furthermore, the prevalence of FV3 in bullfrog farms in Guangdong Province, a major bullfrog farming area in China, was surveyed, and it was found that the detection rate in diseased bullfrogs was 39.5 %. This study will provide effective tools for the early diagnosis and monitoring of FV3 infections, as well as provide foundational data essential for the prevention and control of the disease in bullfrog aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742527"},"PeriodicalIF":3.9,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into mechanism underlying virulence variations between Vibrio mediterranei strains different in pathogenicity toward bivalves","authors":"Haiyan Zhang ,&nbsp;Xiaohui Zou ,&nbsp;Jingjie Hu ,&nbsp;Sheng Liu ,&nbsp;Congling Fan ,&nbsp;Wenfang Dai ,&nbsp;Zhihua Lin ,&nbsp;Qinggang Xue","doi":"10.1016/j.aquaculture.2025.742524","DOIUrl":"10.1016/j.aquaculture.2025.742524","url":null,"abstract":"<div><div>Larval vibriosis, also known as bacillary necrosis, is a widespread bacterial disease that affects the larval development stages of mollusks. It often results in significant mortality among larvae and juvenile mollusks, leading to considerable economic losses. In 2019, during a mass mortality event of razor clam (<em>Sinonovacula constricta</em>) juveniles at a shellfish hatchery, <em>Vibrio mediterranei</em> was identified as the causative agent of larval vibriosis. Notably, the observed pathogenicity was not a species-specific characteristic of the <em>Vibrio</em> genus. To investigate the pathogenesis of <em>V. mediterranei</em> strains, we performed whole genome sequencing, genomic comparative analysis, and biological comparisons of strains with differing virulence, and explored the putative main virulence factors of <em>V. mediterranei</em>. Our findings showed that <em>V. mediterranei</em> strains exhibited similar GC content (∼44 %) and genomic compositions, including two chromosomes and varying numbers of plasmids. However, the high virulence strains have larger genome size, more genes, prophages and virulence-associated genes. Additionally, <em>V. mediterranei</em> strains with different virulence exhibited varying capacities for adhesion and biofilm formation on solid surfaces. Genomic comparisons revealed a strain-specific pathogenicity island (PAI), termed TCP-PAI, in high virulence strains. This PAI shared a similar genomic structure and amino acid identity with the key virulence factor, the TCP gene cluster, found in the human pathogen <em>Vibrio cholerae</em>. A comparative analysis of twenty-one <em>V. mediterranei</em> strains further confirmed the presence of TCP-PAI in different pathogenic strains, highlighting the role of TCP-PAI in the pathogenicity of <em>V. mediterranei</em> toward bivalves. This study is the first to identify a key virulence factor in <em>V. mediterranei</em> linked to the TCP gene cluster of <em>V. cholerae</em>, providing a basis for further investigation into the pathogenic mechanisms of vibriosis in bivalves.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742524"},"PeriodicalIF":3.9,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetrahydropalmatine exhibits anti-parasitic activity: A novel control strategy against Enterocytozoon hepatopenaei infection in shrimp","authors":"Li-Peng Shan ,&nbsp;Mao-Mao Wang ,&nbsp;Yang Hu ,&nbsp;Lei Liu ,&nbsp;Bin Zhu ,&nbsp;Jiong Chen","doi":"10.1016/j.aquaculture.2025.742546","DOIUrl":"10.1016/j.aquaculture.2025.742546","url":null,"abstract":"<div><div>Hepatopancreatic microsporidiosis (HPM), caused by <em>Enterocytozoon hepatopenaei</em> (EHP), has emerged as a major threat to the shrimp aquaculture industry. However, no effective strategies have yet been developed to control the spread of this disease. In this study, we assessed the anti-EHP activity of ten monomeric compounds in <em>Penaeus vannamei</em>. The results showed that tetrahydropalmatine reduced the EHP copy number by 73.8 % at a concentration of 20 mg/L. In a therapeutic experiment, feeding EHP-infected shrimp a diet containing 0.1 % tetrahydropalmatine significantly lowered the EHP load. Additionally, the growth-related gene <em>juvenile hormone esterase-like carboxylesterase 1</em> was significantly upregulated, while <em>ecdysteroid-regulated protein</em> and <em>farnesoic acid O-methyltransferase</em> were significantly downregulated, suggesting that tetrahydropalmatine may enhance the growth potential of EHP-infected shrimp. After 30 days of feeding, the EHP-infected shrimp's body weight in the groups fed with tetrahydropalmatine increased by 19.9 % compared to those fed with the normal diet. In a prevention experiment, healthy shrimp fed a diet containing tetrahydropalmatine and subsequently infected with EHP showed an increase in body weight by day 30 post-infection, compared to the control group, although this difference was not statistically significant. Tetrahydropalmatine can inhibit spore germination and electron dense disc formation, which may be an important reason for its suppression of EHP infection. Moreover, tetrahydropalmatine effectively inhibited the horizontal transmission of EHP, suggesting its potential to limit HPM outbreaks. These findings indicate that tetrahydropalmatine may serve as a feed additive for the treatment of HPM caused by EHP.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742546"},"PeriodicalIF":3.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of intertidal air exposure and handling husbandries on shell development, meat condition and survival of farmed Pacific oysters (Magallana gigas, Thunberg 1793)","authors":"Ernest O. Chuku ,&nbsp;Gregory G. Smith ,&nbsp;Debashish Mazumder ,&nbsp;Steven A. Rust ,&nbsp;Andrew J. Trotter","doi":"10.1016/j.aquaculture.2025.742530","DOIUrl":"10.1016/j.aquaculture.2025.742530","url":null,"abstract":"<div><div>Oyster farming practices are advancing towards optimising production amid shifting market demands and changing climates. This requires a refined understanding of the complexities in handling strategies involving air exposure via suspended line or off-bottom culture and handling techniques on production and market qualities of the oyster. This study investigated the interactive effects of air exposure (0, 4, 11.5 h d⁻¹) and handling/grading frequencies (0, 4, and 7 x) over a 350-d culture period on the shell quality, growth, survival, meat condition and biofouling in Pacific oysters. Shell quality metrics included weight, thickness, chalkiness, compressive strength, density, breakage, broadness and cup depth indices, roundness, and exterior colour hue. Subtidal oysters (0 h air exposure), characterised by stable, warmer temperatures, higher salinity and chlorophyll abundance, exhibited accelerated growth. This group attained larger sizes with thicker shells of lighter hue, albeit with reduced shell density and increased chalkiness, predisposing them to greater fragility. Conversely, prolonged air exposure (11.5 h d⁻¹), characterised by fluctuating, averagely cooler temperatures, reduced salinity and higher turbidity water, resulted in the smallest oysters with the densest, broadest but shallower-cupped and darkest hued shell exteriors. A moderate (4 h d⁻¹) air exposure yielded an intermediate shell with superior compressive strength and thickness. While the interactive effect of air exposure and handling on shell quality was nuanced, a hierarchical influence on survival was observed. Air exposure demonstrated a positive influence on survival and condition index while negatively influencing fouling. The potential physiological mechanisms underlying variations in shell quality are discussed. There is the potential to optimise shell quality in oyster production through a judicious combination of moderate to high air exposure regimes with carefully defined handling protocols. A chequerboard of options is provided.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742530"},"PeriodicalIF":3.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of phycospheric microbial diversity and metabolome between vegetative and matured conchocelis of Pyropia haitanensis","authors":"Rui Zhao ,&nbsp;Zenghu Zhang ,&nbsp;Fuli Liu ,&nbsp;Qikun Xing ,&nbsp;Ningning Xu ,&nbsp;Wenjie Yan ,&nbsp;Xu Gao","doi":"10.1016/j.aquaculture.2025.742515","DOIUrl":"10.1016/j.aquaculture.2025.742515","url":null,"abstract":"<div><div><em>Pyropia haitanensis</em>, relies heavily on conchocelis maturation for its production. However, the prolonged conchocelis development time limits the factory breeding of <em>P. haitanensis</em>. The development of algae is closely concerned in phycospheric microbial communities, yet little is known about the variations in these microbial communities across different conchocelis life stages. This study employed 16S rRNA, ITS gene sequencing and untargeted metabolomics to compare and analyze the phycospheric microbial communities and metabolites between filamentous conchocelis and sporangia branchlet stages. We identified 19 microorganisms with significantly different abundances and established a significant positive correlation with 10 key metabolites. These findings suggested that phycospheric microorganisms actively participated in metabolite synthesis during conchocelis maturation, influencing development, defense response, hormone levels, sulfur metabolism, protein and photosynthetic pigment synthesis. The differential microorganisms between filamentous conchocelis and sporangia branchlet stages were a vital factor in metabolite accumulation, highlighting the interconnectedness between phycospheric microorganisms and metabolites. Our research provided theoretical guidance for utilizing beneficial microorganisms to facilitate conchocelis maturation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742515"},"PeriodicalIF":3.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary trehalose reduced liver glycogen accumulation and promoted protein deposition in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) fed a high-carbohydrate diet","authors":"Yi Xiao ,&nbsp;Xingbo Su ,&nbsp;Jiaying Xie,&nbsp;Huiwen Zhang,&nbsp;Qiming Huang,&nbsp;Douglas R. Tocher,&nbsp;Fan Lin,&nbsp;Cuiying Chen,&nbsp;Shuqi Wang,&nbsp;Ruixin Li","doi":"10.1016/j.aquaculture.2025.742544","DOIUrl":"10.1016/j.aquaculture.2025.742544","url":null,"abstract":"&lt;div&gt;&lt;div&gt;The present study investigated the primary factors contributing to liver dysfunction induced by high-carbohydrate diets and assessed the potential of trehalose in mitigating these effects in hybrid grouper (&lt;em&gt;Epinephelus fuscoguttatus♀ × E. lanceolatus♂&lt;/em&gt;). During an eight-week feeding trial, hybrid grouper were fed either a control diet or a high-carbohydrate (HC) diet supplemented with trehalose at concentrations of 0 %, 1.5 %, and 3 %. Body weight was significantly lower in fish fed HC alone compared to fish fed the control diet, but was increased significantly by supplementing the HC diet with 3 % trehalose. While liver total lipid and triglyceride contents, and mesenteric fat index were not increased in fish fed HC alone compared to fish fed the control diet, addition of 3 % trehalose to the HC diet significantly increased these parameters. Although trehalose supplementation increased peroxisome proliferator-activated receptor α (&lt;em&gt;pparα)&lt;/em&gt; gene expression in liver, it significantly inhibited hepatic lipolysis in fish fed HC alone. Blood glucose and liver glycogen levels were significantly higher in grouper fed HC alone compared to the control group, and the supplementation of HC with trehalose (1.5 % and 3 %) further elevated blood glucose levels, but markedly reduced liver glycogen content. Liver expression levels of insulin receptor (&lt;em&gt;ir&lt;/em&gt;), glucose transporter 2 (&lt;em&gt;glut2&lt;/em&gt;), glycogen synthase (&lt;em&gt;gs&lt;/em&gt;) and glucokinase (&lt;em&gt;gk&lt;/em&gt;) were increased significantly in grouper fed HC alone compared to fish fed the control diet and addition of 3 % trehalose reduced these levels, other than &lt;em&gt;glut2&lt;/em&gt;. Supplementation of 3 % trehalose to HC also reduced liver expression of pyruvate kinase (&lt;em&gt;pk&lt;/em&gt;) and glucose-6-phosphatase (&lt;em&gt;g6pase&lt;/em&gt;), but increased significantly the expression of glycogen phosphorylase (&lt;em&gt;gp&lt;/em&gt;), while the expression of these genes was not different between fish fed HC alone and control diets. Serum alanine aminotransferase and aspartate aminotransferase activities were elevated significantly in fish fed HC alone compared to control diet, and decreased significantly by supplementation with 3 % trehalose. Additionally, liver malondialdehyde levels were increased in fish fed HC alone compared to control diet, but reduced significantly in fish fed HC supplemented with 3 % trehalose. While crude protein contents of liver and muscle were not impacted significantly by HC alone compared to the control diet, they were increased significantly by trehalose supplementation, with liver expression levels of mTORC1 and S6 proteins enhanced significantly by 3 % trehalose. Moreover, microbiota analysis showed increased abundance of &lt;em&gt;Cetobacterium&lt;/em&gt; in the intestine of grouper fed the trehalose-supplemented diets. In summary, the results indicated that HC led to glycogen rather than lipid accumulation in liver, while trehalose treatment mitigated HC-induced glycogen accumulation by inh","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742544"},"PeriodicalIF":3.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing growth, pigment composition, polysaccharides, fatty and amino acid content in Ulva prolifera by manipulating temperature, light intensity, and photoperiod","authors":"Xuan Zhou ,&nbsp;Mingzi Lin ,&nbsp;Jianan Jiang ,&nbsp;Shenyuan Liang ,&nbsp;Han Xu ,&nbsp;Wenrong Zhu ,&nbsp;Nianjun Xu ,&nbsp;Yahe Li","doi":"10.1016/j.aquaculture.2025.742529","DOIUrl":"10.1016/j.aquaculture.2025.742529","url":null,"abstract":"<div><div><em>Ulva</em> spp., a type of cultivated species of seaweed, are characterized by the high carbon and protein content. In this study, a strain of <em>Ulva prolifera</em> named “\"Yonghu 002\" was collected and characterized from Xiangshan Bay, Ningbo, Zhejiang Province. Subsequently, its gamates were induced and collected, then the seedlings were obtained by using the \"germing clusters\" method. Next, the seedlings were cultured under varying temperatures (14, 18, 22, 26, and 30 °C), light intensities (50, 100, 250, 350, 450, and 600 μmol m⁻² s⁻¹), and photoperiods (4 h: 20 h, 8 h: 16 h, 12 h: 12 h, 16 h: 8 h, 2 0 h: 4 h, and 24 h: 0 h, L: D), respectively. The growth, total polysaccharides, fatty acids, and amino acids were evaluated. The results indicated that the optimal conditions for the growth and quality of Yonghu 002 were 100 μmol m⁻² s⁻¹ with 12 h: 12 h (L: D) at 18 and 22 °C. Under these conditions, the soluble protein content was up to 4.42 mg g⁻¹ FW (Fresh Weight), the total polysaccharide content was as high as 7.09 mg g⁻¹ FW, the ratio of unsaturated fatty acids to saturated fatty acids was approximately 1.80, and the ratio of fresh amino acids to total free amino acids was high. This study lays the groundwork for the land-based cultivation of <em>Ulva</em> spp. and provides a theoretical foundation for their utilization as biological resources.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742529"},"PeriodicalIF":3.9,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}