Aquaculture最新文献

{"title":"Freshwater compared to marine fish - A quantitative study on consumer perspectives","authors":"Tamás Lámfalusy ,&nbsp;László Ózsvári ,&nbsp;Dávid Szakos ,&nbsp;Gyula Kasza","doi":"10.1016/j.aquaculture.2025.742382","DOIUrl":"10.1016/j.aquaculture.2025.742382","url":null,"abstract":"<div><div>Although the health benefits of fish consumption are widely acknowledged, annual fish consumption in Hungary remains just a quarter of the EU-27 average. Despite the country's relatively developed freshwater aquaculture, freshwater fish is still a tertiary choice compared to poultry, pork, and, in many cases, marine fish. This study investigates the underlying reasons for the dispreference of freshwater fish relative to marine fish across various consumer groups and identifies the key barriers that aquaculture stakeholders must address to significantly boost consumption. This research is based on a representative survey (<em>n</em> = 1001) conducted between May and June 2021, reflecting Hungary's demographic diversity in age, residence, and gender. A combination of descriptive statistics, Fisher's exac<em>t-</em>test, paired t-test, and One-Way ANOVA F-test with Tukey post hoc analysis was applied to assess consumer motives and consumption barriers. The results show that marine fish is consumed more frequently than locally produced freshwater fish. While Hungarian consumers view freshwater fish as cheaper, safer, and more environmentally sustainable, the perceived health benefits and superior presentation of marine fish products, especially in canned and ready-to-cook formats, make marine fish a more popular choice. Additionally, the presence of fishbones and inconsistent quality were noted as key disadvantages of freshwater fish. Demographic differences revealed that younger consumers prefer ready-to-eat options, while older generations favour traditional recipes. In summary, although freshwater fish are perceived positively, significant promotional efforts and strategic advertising tailored to age-specific preferences are needed to bridge the fish consumption marketing gap in Hungary.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742382"},"PeriodicalIF":3.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic exploration of the effect of tetrodotoxin on metabolism in Takifugu rubripes ovaries","authors":"Yuchun Lu ,&nbsp;Teng Teng ,&nbsp;Ziqiao Zhao ,&nbsp;Yuan Ji ,&nbsp;Baoliang Liu","doi":"10.1016/j.aquaculture.2025.742389","DOIUrl":"10.1016/j.aquaculture.2025.742389","url":null,"abstract":"<div><div>Fish from the Tetraodontidae family offer compact and flavorful meat, and are increasingly in demand in East and South Asian markets, including China, Japan, South Korea, and India due to their high nutritional, practical, and economic value. <em>Takifugu rubripes</em> is currently one of the most economically important farmed pufferfish in China, Japan, and South Korea. The accumulation of tetrodotoxin (TTX)—a lethal neurotoxin—in pufferfish has gained considerable attention in aquacultural animal breeding research. However, research on the effects of TTX on gonadal metabolism in <em>T. rubripes</em> is limited. To address this research gap, in this study, we used metabolomics and other techniques to conduct a preliminary exploration of how TTX affects ovarian metabolism in <em>T. rubripes</em>. The results revealed that TTX improved the ovarian tissue developmental quality and metabolism in <em>T. rubripes</em> by enhancing the activity of functional enzymes such as superoxide dismutase and lysozyme. These enhancements increased the content of beneficial metabolites such as pregnenolone and reduced the content of harmful metabolites such as indole-3-acetic acid. Overall, this study provides a theoretical basis for improving the quality of ovarian development in <em>T. rubripes</em> parental fish, thereby improving the breeding value and economic potential of the species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742389"},"PeriodicalIF":3.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The involvement of lactate and aspartate in the pro-viral action of glucose on SGIV replication","authors":"Xixi Guo ,&nbsp;Wenji Wang ,&nbsp;Weihua Xu ,&nbsp;Youhua Huang ,&nbsp;Qiwei Qin ,&nbsp;Xiaohong Huang","doi":"10.1016/j.aquaculture.2025.742390","DOIUrl":"10.1016/j.aquaculture.2025.742390","url":null,"abstract":"<div><div>Glycolysis is an energy generating glucose metabolic pathway that is not only critical for cell survival, but also exploited by pathogens to establish a successful infection in host cells. Recently, our study demonstrated that glucose exerted pro-viral action upon Singapore grouper iridovirus (SGIV) infection in vitro. However, the potential mechanism underlying the regulatory roles of glucose on other metabolic pathways in response to SGIV infection remained uncertain. Here, metabolomic analysis indicated that carbon metabolism and amino acid metabolism are the most key pathways regulated by glucose during SGIV infection. Specifically, after glucose treatment, SGIV infection increased the abundance of lactate, but decreased that of aspartate. Interestingly, supplemental lactate significantly promoted SGIV replication, demonstrated by the accelerated cytopathic effect (CPE) severity, increased viral gene transcription, protein synthesis and viral yields. Moreover, the addition of lactate increased the transcription levels of pro-inflammatory cytokines induced by SGIV. In contrast, the addition of aspartate inhibited SGIV replication, evidenced by the weakened CPE severity, reduced viral gene transcription, protein synthesis and viral yields. Aspartate supplementation also reduced the transcription levels of pro-inflammatory cytokines during SGIV infection. Thus, we speculated that the regulatory roles of glucose on lactate and aspartate metabolism might directly contribute to the pro-viral action of glucose upon SGIV infection. Our findings will provide new insights into understanding the roles of glucose metabolism in response to fish virus infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742390"},"PeriodicalIF":3.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses suggest distinct master sex determination genes in closely related blue catfish and channel catfish","authors":"Tong Chen ,&nbsp;Dongya Gao ,&nbsp;Baofeng Su ,&nbsp;Rex Dunham ,&nbsp;Zhanjiang Liu","doi":"10.1016/j.aquaculture.2025.742364","DOIUrl":"10.1016/j.aquaculture.2025.742364","url":null,"abstract":"<div><div>Channel catfish (<em>Ictalurus punctatus</em>) is the most important aquaculture species in the United States. An interspecific hybrid made from female channel catfish and male blue catfish is increasingly becoming the predominant genotypes in the catfish industry. However, artificial hybridization is required because of reproductive isolation. While sex determination and its epigenetic regulation is well studied for channel catfish, no work has been done in blue catfish concerning its sex determination. In this study, as a first step in understanding reproductive isolation and the molecular basis of sex determination and differentiation in blue catfish, we conducted comparative transcriptome analyses of channel catfish and blue catfish, using RNA-Seq analysis of the gonad tissues. Blue catfish sex determination was speculated to be similar to that of channel catfish because they are closely related, and because its sex determination locus was also mapped to chromosome 4. However, RNA-Seq analysis revealed that hydin, the candidate master sex determination (MSD) gene of channel catfish, is not differentially expressed between males and females in blue catfish. The expression profiles of the genes within the channel catfish sex determination region (SDR) are drastically differentially expressed in blue catfish. Among the genes within the SDR, a total of 90 genes were differentially expressed in channel catfish, while only 52 were differentially expressed in blue catfish. It is important to note that of the differentially expressed genes in channel catfish, 19 are non-coding RNAs, while only one non-coding RNA was annotated in blue catfish in this region. This difference in annotation was caused by the shortage of transcriptome datasets from blue catfish. However, there are still 71 differentially expressed protein-coding genes in channel catfish but only 51 differentially expressed protein-coding genes in blue catfish. Global gene expression profiles of channel catfish and blue catfish, especially in terms of differentially expressed genes between males and females, are also highly different between the two species, suggesting that blue catfish may have different sex determination mechanisms, and explaining, at least partially, the molecular basis of reproductive isolation. Future studies are warranted to identify the MSD in blue catfish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742364"},"PeriodicalIF":3.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microbiome, metabolome, and correlation analysis reveal the beneficial role of the commensal Shewanella sp. MR-7 in foodborne enteritis in turbot (Scophthalmus maximus L.)","authors":"Chao-Qun Li ,&nbsp;Wen-Yi Wang ,&nbsp;Xin Wen ,&nbsp;Si-Yu Qi ,&nbsp;Jia-Ning Xu ,&nbsp;Dan-Yu Wu ,&nbsp;Kang-Di Tian ,&nbsp;Chun-Yang Zhang ,&nbsp;Bei-Li Zhang ,&nbsp;Qin-Yuan Ma","doi":"10.1016/j.aquaculture.2025.742387","DOIUrl":"10.1016/j.aquaculture.2025.742387","url":null,"abstract":"<div><div>The mechanism by which commensal bacteria are associated with fish enteritis has attracted much attention. In this work, the role of the commensal <em>Shewanella</em> sp. MR-7 in foodborne enteritis in turbot was analyzed with regard to intestinal morphology, tight junctions, the mucus barrier, the microbiota, and the metabolome. In total, 180 fish were fed a regular fish meal diet (FD), a high soybean meal diet (HSD), or an HSD plus 10⁹ CFU g⁻¹ MR-7 (HSDR) for 47 days. The results showed that MR-7 administration markedly improved the weight gain rate, specific growth rate and feed conversion ratio, which were decreased by the HSD. Villus atrophy, lamina propria widening, and inflammatory cell infiltration were induced by HSD, whereas MR-7 alleviated these pathological symptoms. Further assessment revealed that MR-7 significantly alleviated HSD-mediated intestinal barrier dysfunction through up-regulating tight junction-related <em>Tricellulin</em> and <em>Claudin-3</em> and downregulating mucus barrier-related <em>MUC-2</em>. Moreover, relatively more goblet cells in HSDR group could be observed relative to HSD group. MR-7 administration considerably enriched the genus <em>Shewanella</em> and resulted in significant gut microbial regulation at the genus and phylum levels, ultimately resulting in a greater balance. As shown by metabolomic analysis, 13 differential metabolites between the HSD and HSDR groups were located in 16 KEGG pathways at level 2, and they were mostly associated with amino acid and lipid metabolism. There were 10 different metabolites involved in the top 20 KEGG pathways at level 3, and the most relevant pathways were primary bile acid biosynthesis, histidine metabolism, glycosylphosphatidylinositol-anchor biosynthesis, autophagy, and thyroid hormone synthesis. Pearson correlation analysis revealed the crosstalk between differential bacterial flora and metabolites, revealing that <em>Mycobacterium</em> was the key genus and <em>N</em>-acetylhistamine, cholic acid, and citric acid were key biomarkers involved in the positive role of MR-7 in relieving turbot enteritis. To summarize, the findings suggested that the commensal MR-7 can alleviate foodborne enteritis in turbot by enhancing their histological characteristics, protecting tight junctions and the mucus barrier, and maintaining microbiota and metabolic homeostasis.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742387"},"PeriodicalIF":3.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early detection of sea lice epidemic transitions and changes in long-term abundance levels in salmon farming areas","authors":"Rodrigo M. Montes ,&nbsp;Renato A. Quiñones","doi":"10.1016/j.aquaculture.2025.742385","DOIUrl":"10.1016/j.aquaculture.2025.742385","url":null,"abstract":"<div><div>Early warning indicators (EWIs) have been applied within the framework of dynamical systems theory to detect in advance tipping points in stressed or perturbed ecosystems, including the early detection of disease outbreaks. Early warning signals in epidemiology occur when an epidemic transition point is approached, marking the transition in the state of an epidemic. Here, EWIs were used to detect large outbreaks of sea lice (<em>Caligus rogercresseyi</em>) in advance using time series of total adult abundance from a highly infested salmon farming area in southern Chile. Generic early warning indicators successfully detected increases in <em>C. rogercresseyi</em> abundance several months in advance of two major transition points marked by: i) El Niño 2016, ii) abnormal climatic/oceanographic conditions during the spring-summer of 2019–2020. The emergence of a trend in EWIs was interpreted as an early response of <em>C. rogercresseyi</em> to oceanographic disturbances. New signatures, characterized by a marked loss of long-term memory and predictability, and an increase in randomness in sea lice abundance time series were detected using the Hurst coefficient (H) and the weighted permutation entropy (WPE). An opposite pattern of variability between H and WPE was observed; and a segmented regression identified major breakpoints in the overall sea lice abundance dynamics as H decreased. Because H and WPE track the long-term dynamics of sea lice abundance well, we proposed them as complementary indicators to aid in the management and control of increasing sea lice epidemic outburst, as they appear to track the developed resistance of sea lice to anti-lice chemicals.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742385"},"PeriodicalIF":3.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sufficient burial depth is a prerequisite for Chinese cyclina (Cyclina sinensis) to maintain normal life activities","authors":"Yurou Ma ,&nbsp;Shiyu Yan ,&nbsp;Zhengyu Shao ,&nbsp;Guolong Zhou ,&nbsp;Ang Hu ,&nbsp;Yawei Bu ,&nbsp;Huimin Wang ,&nbsp;Xinqi Lv ,&nbsp;Jianyu Liu ,&nbsp;Yihua Chen ,&nbsp;Zhiguo Dong","doi":"10.1016/j.aquaculture.2025.742381","DOIUrl":"10.1016/j.aquaculture.2025.742381","url":null,"abstract":"<div><div>Substrates play a crucial role in the life activities of many bivalves with a burrowing lifestyle. Substrate effects were investigated by observing changes in the growth, physiology, and shell morphology of <em>Cyclina sinensis</em> growing on unsuitable substrates. There were five treatment groups: no substrate (NS); no substrate but <em>C. sinensis</em> could simulate burrowing behavior (NSB); and substrate depths at half the shell length (SL, 1/2 × SL), equal to the SL (1 × SL), and three times the SL (3 × SL, control). <em>Cyclina sinensis</em> survival in NS, NSB, and 1/2 × SL was significantly lower than in the control group (<em>P</em> &lt; 0.05) and growth in all treatment groups was significantly lower than in the control group (<em>P</em> &lt; 0.05). <em>Cyclina sinensis</em> in NS initially grew rapidly but were ultimately surpassed by the 1 × SL group. The poor growth observed in the 1/2 × SL group may be attributed to the energy consumed by their movement. Unsuitable substrates had minimal impacts on gonadosomatic development, but significantly affected soft tissue and hepatopancreas development. This also reduced the activity of digestive enzymes including amylase and protease. Moreover, both the filtration rate (FR) and ingestion rate (IR) declined, these factors collectively contributed to the inevitable weakening of its energy assimilation. The superoxide dismutase and catalase activities significantly increased when the substrate conditions were unsuitable, indicating that the antioxidant system was under stress. Except for the FR and IR in NSB group, which were superior to those in NS and 1/2 × SL groups, all other indicators were the worst. Both cluster and discriminant analyses showed that shell morphology in NS was significantly different from that of the other groups. Therefore, to ensure the normal life activities, the depth of the substrate must exceed three times the SL of the <em>C. sinensis</em>. This research will be of great guiding significance for the scientific selection of the cultivation environment of <em>C. sinensis</em> to improve economic efficiency.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742381"},"PeriodicalIF":3.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prophylactic sarafloxacin hydrochloride increases shrimp susceptibility to AHPND via transferring antibiotic resistance genes and virulence factors","authors":"Haonan Sha ,&nbsp;Xinxu Zhang ,&nbsp;Jiong Chen ,&nbsp;Qiongfen Qiu ,&nbsp;Jinbo Xiong","doi":"10.1016/j.aquaculture.2025.742379","DOIUrl":"10.1016/j.aquaculture.2025.742379","url":null,"abstract":"<div><div>The abuse of prophylactic antibiotics could exert selective pressure on the shrimp <em>Litopenaeus vannamei</em> gut microbiota, as well as the emergence and spread of antibiotic resistance genes (ARGs). However, the underlying molecular mechanism of ARGs transfer remains unexplored. Here, we unveiled how prophylactic sarafloxacin hydrochloride (SAR) affected the transfer of ARGs and subsequent disease susceptibility. Results showed that 7 days of SAR exposure significantly altered the gut microbiomes and reduced microbial diversity, resulting in increased susceptibility to subsequent <em>Vibrio anguillarum</em> (a causing pathogen of acute hepatopancreatic necrosis disease, AHPND) infection. SAR exposure plus infection diversified the shrimp gut pathogens and virulence factors, which caused faster and higher mortality. In addition, SAR exposure enriched various ARGs including non-quinolone ARGs, indicating the co-selection of unrelated ARGs with unexpected risk. SAR exposure plus infection additively stimulated the dissemination of ARGs through conjunctive transfer in the shrimp gut microbiota. Network analysis further depicted the emergence of pathogens hosting multiples virulence factors and ARGs, including pathogens in shrimp (e.g., <em>V. parahaemolyticus</em>) and for humankind, e.g., <em>Haemophilus ducreyi</em> and <em>Helicobacter pylori</em>. Collectively, prophylactic antibiotic directly affects shrimp gut microbiomes and mobile genetic elements (MGEs), which stimulate the dissemination of ARGs and virulence factors. The potentiated virulence and resistance of pathogens cause rapid and high mortality of shrimp. These findings depict a new perspective on the molecular mechanisms of ARGs and virulence factors dissemination in the shrimp gut, as well as shrimp susceptibility to AHPND.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742379"},"PeriodicalIF":3.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented spent coffee ground in African catfish (Clarias gariepinus) diets: Effects on growth performance, digestive enzyme, protein digestibility, amino acid profile, and immune-related gene","authors":"Nurdiyana Aqilah Roslan ,&nbsp;Zulhisyam Abdul Kari ,&nbsp;Suniza Anis Mohamad Sukri ,&nbsp;Martina Irwan Khoo ,&nbsp;Nani Izreen Mohd Sani ,&nbsp;Roslina Rashid ,&nbsp;Muhammad Anamul Kabir ,&nbsp;Shishir Kumar Nandi ,&nbsp;Nik Nur Azwanida Zakaria ,&nbsp;Koushik Ghosh ,&nbsp;Hasnita Che Harun ,&nbsp;El-Sayed Hemdan Eissa","doi":"10.1016/j.aquaculture.2025.742383","DOIUrl":"10.1016/j.aquaculture.2025.742383","url":null,"abstract":"<div><div>The application of fermented spent coffee ground (FCG) as animal feed has been documented by multiple prior studies, while others have explored its potential as aquafeed. The current research investigated the influence of FCG as a protein substitution at various levels (0 %, 10 %, 20 %, 40 %, and 80 %) on the growth, health, digestive enzymes, protein digestibility, amino acid profile, and gene expression of African catfish (<em>Clarias gariepinus</em>). Five dietary formulations were designed, and FCG was incorporated at different levels: 0 % FCG/control (T1); 10 % FCG (T2); 20 % FCG (T3); 40 % FCG (T4); 80 % FCG (T5). The fish were divided into 15 tanks (350 L) for a feeding trial that lasted for 70 days (<em>n</em> = 70 fish/per tank). The results showed that the T2 group exhibited the most significant enhancement (<em>p</em> &lt; 0.05) under growth conditions relative to other treatments. The apparent protein digestibility (APD) values exhibited variability (p &lt; 0.05), with T2 showing the highest APD, while a decreasing trend was observed from T3 to T5. Among the treatments, fish receiving 10 % FCG displayed the most pronounced activity of the digestive enzymes, protease, lipase, and amylase. In addition, the muscle, liver, and intestinal amino acid composition showed the highest (<em>p</em> &lt; 0.05) in the T2-supplemented group demonstrated the highest total essential amino acids (EAA) and non-essential amino acids (NEAA). Besides, fish fed with the T2 diet exhibited significant (p <em>&lt;</em> 0.05) and highest TGF-β1, NF-kβ, hsp90a and lyzg gene expression compared to other treatments in the fish intestine. To conclude, incorporating FCG as a fishmeal substitute in aquafeed notably improved the growth and health of <em>C. gariepinus</em> in this study. A 10 % inclusion of FCG proved effective for aquafeed, especially for African catfish and other freshwater species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742383"},"PeriodicalIF":3.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A long photoperiod following a short one, and low temperature, stimulate gametogenesis in the sea cucumber Holothuria arguinensis","authors":"Nathalie Marquet,&nbsp;Adelino V.M. Canário","doi":"10.1016/j.aquaculture.2025.742377","DOIUrl":"10.1016/j.aquaculture.2025.742377","url":null,"abstract":"<div><div>Temperature and photoperiod are critical regulators of reproduction. However, few studies have evaluated experimentally their specific effects on gametogenesis in sea cucumbers. We tested the effect on gametogenesis of combinations of photoperiod and temperature: “accelerated” (long days following short days; a 12 °C deep in temperature), “constant” (constant short photoperiod; constant Spring/Summer temperature) and “natural” conditions (natural photoperiod and temperature). Experiment 1 started in mid-November (after gametogenesis began) and lasted until March. Experiment 2 started in September, after the spawning season (before gametogenesis started), with gonadal biopsy and spawning trials between March and May. In both experiments, the most advanced gonad development was found in accelerated photoperiod and temperature, with more than 50 % of the sea cucumbers reaching maturity and having the longest and thickest gonadal tubules. However, gametogenesis could not progress in sea cucumbers (i.e.<em>,</em> shortest and thinnest gonad tubules) under constant short photoperiod combined with accelerated temperature. This indicates that a constant short photoperiod inhibits gametogenesis and that an accelerated thermal cycle cannot trigger gonad development alone. When accelerated photoperiod was combined with either accelerated or constant temperature, gametogenesis progressed, advancing faster in accelerated than constant temperature, with spawning only seen in these two treatments. This highlights that long days following short days are required for gametogenesis and that temperature has a modulatory role rather than a trigger, with a cool winter period being stimulatory. Altogether, our study provides essential information into environmental factors in sea cucumber reproduction, valuable for broodstock management and sustainable aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"603 ","pages":"Article 742377"},"PeriodicalIF":3.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}