Aquaculture最新文献

{"title":"Comparing the modulatory effect of fennel powder and fennel extract nanoparticles on growth, immunity and histopathological changes of Nile tilapia","authors":"Shaimaa A.A. Ahmed ,&nbsp;Abdelhakeem El-Murr ,&nbsp;Yasser Abd Elhakim ,&nbsp;Elsayed M. Younis ,&nbsp;Abdelwahab A. Abdelwarith ,&nbsp;Simon J. Davies ,&nbsp;Mohamed M. Metwally ,&nbsp;Amany Abd El Aziz Gharib ,&nbsp;Enas N.M. Khalil","doi":"10.1016/j.aquaculture.2025.742566","DOIUrl":"10.1016/j.aquaculture.2025.742566","url":null,"abstract":"<div><div>A sixty-day feeding trial was performed to assess the impact of fennel powder (“FP) and fennel extracts nanoparticles (FNPs) in enhancing the growth performance, antioxidant capacity. The immune system's ability to fend against <em>P. putida</em> infection was also assayed. Nile tilapia (<em>n</em> = 225, 27.15 ± 0.12 g) were segregated into five equal groups in triplicates (45 fish/ group, 15 fish/ replicate). The initial (control) group received diet devoid of any feed supplements, whereas the second (FP 0.5) and third (FP 1) ones received diet enriched with 0.5 % and 1 % fennel powder (FP) respectively. The fourth (FNPs 0.5) and the fifth (FNPs 1) groups received a basal diet enriched with 0.5 % and 1 % (FNPs) respectively. At the end of feeding trial; fifteen fish from each group, were intraperitoneally (I/P) inoculated with <em>P. putida.</em> The challenged fish were monitored for fourteen days, where mortalities, behavioral modification and clinical signs were documented<em>.</em> Results depicted that, FP and/or FNPs- enriched groups showed a substantial improvement in all growth indices compared to the control group. The immunological response biomarkers involving lysozyme (32.07 μg/mL), NO (47.67 μmol/L), and IgM (40.26 μg/mL) were uniquely improved in the FNPs 1 group when compared to their values in the control group(16.37, 34.88, and 26.28, respectively). In addition, FNPs 1group also displayed major improvement in the activity of hepatic oxidative stress biomarkers: CAT (22.93 U/G), SOD (9.17 U/g), and GSH (2.92 mmol/g). Following the challenge with <em>P. putida</em>, the highest survivability percentage was noted in FNPs 1 (88.66 %), followed by FNPs 0.5 (73.33 %), and then, FN1 (66.66 %). Eventually, it could be concluded that FNPs, particularly, with higher concentrations could be used as a promising dietary supplement to enhance growth and immunological response as well as to safeguard Nile tilapia against <em>P. putida</em> infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742566"},"PeriodicalIF":3.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Descaling did not affect osmoregulation, growth or mortality in Atlantic salmon smolts after transfer to brackish or sea water","authors":"Jonatan Nilsson,&nbsp;Angelico Madaro","doi":"10.1016/j.aquaculture.2025.742526","DOIUrl":"10.1016/j.aquaculture.2025.742526","url":null,"abstract":"<div><div>Scale loss is often a result of various mechanical impact such as handling and is assumed to increase the risk of impaired osmoregulation and disease. For this reason, scale loss is a commonly used welfare indicator for farmed fish. Knowledge about the consequences scale loss has for fish welfare under different conditions is however limited, especially the long-term effects. We studied the effect of removal of scales on 0 (control) 5, 10 or 20 % of the scale covered body area on individually tagged Atlantic salmon (<em>Salmo salar</em>, Linnaeus) smolts. Descaling was done at the transfer from freshwater to either brackish (25 ppt) or full-strength seawater (34 ppt) at an optimal temperature (10 °C) that was kept stable during the 6-weeks study period. Fish were sampled for blood and examined for wounds after 1, 7 and 42 days. No fish had wounds at any of the sampling occasions, and no fish in any treatment died during the experimental period. Although all plasma parameters measured (concentration of plasma cortisol, glucose, lactate, Cl⁻, Na⁺, K⁺, and Ca²⁺) were affected by sampling day and/or salinity, scale loss did not have a significant effect on any of the plasma parameters. Neither did scale loss affect growth. Thus, no negative effects of scale loss on salmon welfare could be documented in the present study. Scale loss may still have negative effects in more challenging conditions, which should be investigated in more detail.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742526"},"PeriodicalIF":3.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taurine related pathways effect the potential domestication of wild Larimichthys crocea adapt to stress environment","authors":"Xubing Ba ,&nbsp;Zhenjun Zhuang ,&nbsp;Zebin Zhou ,&nbsp;Jiayu Zhou ,&nbsp;Qianwen Min ,&nbsp;Biao Yuan ,&nbsp;Xin Yi ,&nbsp;Ruojing Li ,&nbsp;Zhaoqiu Qu ,&nbsp;Na Zhao ,&nbsp;Bo Zhang","doi":"10.1016/j.aquaculture.2025.742532","DOIUrl":"10.1016/j.aquaculture.2025.742532","url":null,"abstract":"<div><div>The large yellow croaker (<em>Larimichthys crocea</em>) was inherently sensitive and exhibited pronounced responses to external stimuli, which posed challenges for its domestication and breeding. The swim bladder, a sensory organ richly innervated with sympathetic and parasympathetic nerves, played a critical role in this process. However, the molecular mechanisms underlying the neural and physiological changes induced by domestication remained unclear. In this study, significant differences in metabolic profile and gene expression were identified in swim bladder when compared cultured and wild <em>L. crocea</em>, as well as in metabolic profile of liver. Taurine and hypotaurine metabolism were notably enriched in the swim bladder and liver. Taurine levels were significantly higher in the swim bladder of wild individuals, and after injection of taurine, gene expression levels of taurine synthesis in swim bladder were elevated, while taurine transport and metabolism genes in swim bladder and liver were also upregulated. In addition, taurine alterations were associated with differential expression of neuroregulatory genes. Swim bladder inflation and deflation resulted in varying degrees of reduction or deformation of the Nissl bodies and elevated expression of neural regulatory genes after pressure and noise exposure. Pressure and noise processing affected neural regulatory genes such as <em>angpt2</em>, <em>lepr</em>, <em>foxm1</em> and <em>sema3d</em>. These genes showed minimal changes in expression during stress and noise processing, with noise having a greater impact on neural regulation in the brain. Taurine injection significantly increased expression of <em>angpt2</em>, <em>lepr</em>, <em>foxm1</em> and <em>sema3d</em> under pressure stress but decreased them under noise stress. Conversely, <em>notch1a</em>, <em>tmc1</em>, <em>colla1a</em>, and <em>mylpfa</em> were consistently downregulated in the swim bladder and brain under both stress conditions. This study was the first to propose a swim bladder-brain axis regulating neural sensitivity in L. <em>crocea</em>, providing new insights into the molecular mechanisms through which domestication and aquaculture affected the neural responses of this species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742532"},"PeriodicalIF":3.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of state-of-the-art improvements in recirculating aquaculture systems: Insights into design, operation, and statistical modeling approaches","authors":"Subha M. Roy ,&nbsp;Hyunsoo Choi ,&nbsp;Taeho Kim","doi":"10.1016/j.aquaculture.2025.742545","DOIUrl":"10.1016/j.aquaculture.2025.742545","url":null,"abstract":"<div><div>In sustainable aquaculture, optimizing yields, water quality, and resource management is crucial. However, in recirculating aquaculture systems (RASs), the accumulation of hazardous substances such as ammonia, nitrite, and nitrate, resulting from uneaten feed and excreta, can negatively affect the health of farmed organisms, disrupt system operations, and reduce overall productivity. Therefore, RASs must be designed to effectively remove these substances and maintain optimal growth conditions. This review summarizes past efforts to enhance RAS design and operational parameters using various design of experiments (DOE) approaches to improve system performance. DOE is a powerful methodology for optimizing outcomes while minimizing the number of experimental trials. Although selecting the most suitable DOE approach depends on the specific RAS design, few studies have systematically explored its application in RAS optimization. This review addresses this gap, offering valuable guidance to researchers and aquaculture farmers on selecting appropriate DOE methods based on specific RAS requirements.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742545"},"PeriodicalIF":3.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of DPO-based PCR, RT-PCR and LAMP for the monitoring of WSSV and YHV in shrimp aquaculture","authors":"Yen Yew Chan ,&nbsp;SiouNing Aileen See ,&nbsp;Zee Hong Goh ,&nbsp;Subha Bhassu","doi":"10.1016/j.aquaculture.2025.742567","DOIUrl":"10.1016/j.aquaculture.2025.742567","url":null,"abstract":"<div><div>The emergence of viral outbreaks in cultured crustaceans is estimated to have caused losses of more than 3 billion dollars over the past three decades. Despite the implementation of control measures as recommended by OIE, this serious threat to the aquaculture industry has not been successfully mitigated. To effectively address this issue, routine screening of shrimps using established molecular techniques is essential to prevent the spread of pathogens. In this work, Dual Priming Oligonucleotide (DPO)-based multiplex PCR was deployed to screen shrimp viruses (WSSV and YHV) from farm samples, validated with real time PCR as a comparative study. This improved molecular technique, which remains still scarce in aquaculture research, demonstrated higher sensitivity and selectivity than the conventional PCR due to its dual-priming system. Yet, molecular techniques still face many challenges such as longer running time, the inability to perform in-situ diagnostics, the requirement for technical expertise, strict laboratory confinement and high operational cost (including reagents and instruments). As an alternative, we developed a loop mediated isothermal amplification (LAMP) method for the detection of WSSV and YHV shrimp diseases, which has the potential to be integrated into a lab-on-a-chip (LOC) or point-of-care (POC) devices. This assay achieved sensitivities of 1 × 10² copies/μL for WSSV and 1 × 10³ copies/μL for YHV, while demonstrating specificity by exhibiting no cross-reactivity with seven other OIE-listed shrimp viruses. This rapid and thermal cycler-free technique allows visually distinguishable LAMP results making it a practical and accessible diagnostic solution in resource-limited aquaculture settings. This method acts as a biomonitoring tool to improve the detection of shrimp viral diseases in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742567"},"PeriodicalIF":3.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved coral nursery production through contingent heat stress events via depth manipulation","authors":"Joseph A. Henry ,&nbsp;Sebastian Szereday ,&nbsp;Gilles G.R. Bernard ,&nbsp;Joshua T. Patterson","doi":"10.1016/j.aquaculture.2025.742558","DOIUrl":"10.1016/j.aquaculture.2025.742558","url":null,"abstract":"<div><div>Ocean-based coral aquaculture is increasingly employed to generate biomass for active coral reef restoration. As this practice expands in scale and global application, it is important to explore techniques that optimize nursery yield amid climate change and dynamic environmental conditions. Therefore, we constructed three suspended coral nurseries in Malaysia to test whether increasing coral nursery depth protects biomass production from detrimental heat stress impacts while assessing potential trade-offs in growth and survival. The study focused on three coral species: <em>Acropora muricata</em>, <em>Acropora</em> cf. <em>gemmifera</em>, and <em>Montipora aequituberculata</em>. One nursery was maintained at the original source material collection depth of 8.0 m (Shallow), another was lowered to a depth of 15 m (Deep), and the third nursery (Transitional) was gradually transitioned from 8 to 15 m over a 71 day period. Coral growth and survival were monitored for 582 days, representing the longest observational nursery study yet reported in Malaysia. In addition, these data capture the impacts of the first back-to-back coral bleaching events recorded in the region, as well as a period of heavy tropical storm activity (i.e., northeast monsoon season). Ultimately, major findings include (1) species-specific differences in growth and survival across nurseries, (2) <em>A. muricata</em> exhibited the highest growth rates (0.28 ± 0.02 % day⁻¹) in the Shallow nursery, while <em>M. aequituberculata</em> had the lowest (0.16 ± 0.02 % day⁻¹) in the Deep nursery, (3) survival was the highest at 15 m, with <em>A. gemmifera</em> displaying the greatest survival rate (88 %), and (4) significantly lower heat stress response and bleaching in corals cultured in deeper nurseries. These results suggest that depth manipulation could be a viable strategy for mitigating heat stress impacts in coral nurseries while maintaining favorable biomass production. This study provides key insights for optimizing coral restoration efforts in the Indo-Pacific, where climate change increasingly threatens coral reefs.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742558"},"PeriodicalIF":3.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the bipolar aluminum electrocoagulation model (BAEM) on heavy metal concentration, growth, immunological response, and parasitic activity in cultured Dicentrarchus labrax in underground seawater","authors":"Amr Fadel,&nbsp;Abeer A. Moneer,&nbsp;Khaled M. Abdelsalam,&nbsp;Mohamed M. Abdel-Rahim","doi":"10.1016/j.aquaculture.2025.742554","DOIUrl":"10.1016/j.aquaculture.2025.742554","url":null,"abstract":"<div><div>This study assessed the effectiveness of the bipolar aluminum electrocoagulation model (BAEM) as a water treatment method for heavy metal pollution and parasitic load in <em>Dicentrarchus labrax</em>. Over a six-month experiment, the growth and health of <em>D. labrax</em> fingerlings were monitored under two BAEM treatment schedules: T2 (8-h daytime) and T3 (4-h daytime and 4-h night). Basic water physicochemical parameters were analyzed, and ectoparasites' prevalence, infection intensity, and mortality rate were investigated. Additionally, the growth performance and immune response of the BAEM-treated fish were compared to those of the control nontreated T1 fish. Parasitological examination of fresh skin and gill mounts revealed three common ectoparasites: the protozoan <em>Trichodina</em> sp., the monogenean <em>Diplectanum</em> sp., and the digenean <em>Transversotrema</em> sp. The results demonstrated significant improvements in water quality, with reduced turbidity, total dissolved solids, and heavy metal concentrations. The highest reduction percentages for the heavy metals iron, copper, zinc, and lead were observed in the BAEM-treated samples at T3. Additionally, the BAEM-treated groups exhibited lower prevalence and infection intensities of ectoparasites and enhanced growth performance and immune responses compared to the control group T1. This study presents the first evidence of the BAEM model's effectiveness in enhancing physicochemical parameters, reducing ectoparasite prevalence, and improving growth and immunological responses in cultured fish reared in untreated underground seawater. The model's positive outcomes make it recommended for RAS aquaculture systems and areas with high heavy metal concentrations.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742554"},"PeriodicalIF":3.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dynamic features and microbial mechanism of nitrogen transformation in small greenhouse farming system of Pacific white shrimp, Litopenaeus vannamei","authors":"Yong Huang ,&nbsp;Mei Liu ,&nbsp;Keyong Jiang ,&nbsp;Chen Zhong ,&nbsp;Kefeng Xu ,&nbsp;Baojie Wang ,&nbsp;Lei Wang","doi":"10.1016/j.aquaculture.2025.742557","DOIUrl":"10.1016/j.aquaculture.2025.742557","url":null,"abstract":"<div><div>The proliferation of small greenhouse farms for <em>Litopenaeus vannamei</em> cultivation in China represents an emerging dominant paradigm in shrimp aquaculture. However, inorganic nitrogen accumulation in water during cultivation significantly impacts yield and economic benefits. In this study, water quality analysis, particle size analysis, quantitative Real time-PCR and metagenomics were employed to investigate the main nitrogen transformations, the characteristics of surface sediment (SS) and suspended particulate matter (SP), the attached microbial communities, and the response characteristics of the main functional genes in the small greenhouse. The results indicated that the changes in inorganic nitrogen concentration in the small greenhouse exhibited phased characteristics. The increase in the abundance of ammonia-oxidizing and nitrite-oxidizing bacteria reduced the ammonia and nitrite concentrations in water to less than 1 mg/L during the turbid-turned stage. During the maturity stage, the abundance of key denitrification functional genes (<em>nirS</em> and <em>nirK</em>) both increased to more than 1 × 10⁸ copies/g, promoting the removal of nitrate from the water. SS and SP were mainly composed of silt and clay, providing abundant ecological niches for nitrifying and denitrifying bacteria. The metagenomic results indicated that <em>Defluviimonas</em>, <em>Rhodohalobacter</em>, <em>Nitrosomonas</em> and <em>Nitrobacter</em> were the main nitrifying and denitrifying functional microorganisms in SS and SP. These findings demonstrated that SS- and SP-mediated nitrification and denitrification processes served as primary mechanisms for inorganic nitrogen transformation and removal in small greenhouse farming systems. This study provided critical insights into microbial nitrogen transformation processes in small greenhouse shrimp aquaculture, establishing a theoretical framework for enhanced water quality management and aquaculture practices.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742557"},"PeriodicalIF":3.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential effects of inulin on lipid metabolism disorders in spotted sea bass (Lateolabrax maculatus) induced by a high-fat diet","authors":"Fuqiang Quan ,&nbsp;Xiujuan Wang ,&nbsp;Zhangfan Huang ,&nbsp;Luimin Kong ,&nbsp;Yi Lin ,&nbsp;Hao Lin ,&nbsp;Sishun Zhou ,&nbsp;Jianrong Ma ,&nbsp;Yanbo Zhao ,&nbsp;Yunting Zhao ,&nbsp;Longhui Liu ,&nbsp;ZhongBao Li","doi":"10.1016/j.aquaculture.2025.742510","DOIUrl":"10.1016/j.aquaculture.2025.742510","url":null,"abstract":"<div><div>One green and eco-friendly feed additive that has been shown to reduce lipids in organisms is inulin (INU). This study sought to determine how adding INU to a high-fat diet (HFD) affected the spotted sea bass's (<em>Lateolabrax maculatus</em>) growth, digestion, and lipid metabolism. Six sets of three replicate fish each were created from a total of 360 disease-free, healthy spotted sea bass (<em>Lateolabrax maculatus</em>) weighing 2.35 ± 0.04 g, which were then housed in 18 culture tanks. The experimental spotted sea bass were fed the baseline diet (CK), high-fat diet (HFD) and HFD supplemented with 0.5 % INU(INU0.5), 1 % INU(INU1), 1.5 % INU(INU1.5) and 2 % INU(INU2), respectively, for 70 consecutive days. The results demonstrated that in comparison to the HFD group, dietary intake of INU significantly reduced the feed conversion ratio and adiposity in spotted sea bass. However, it did not exert a significant impact on specific growth rate (SGR), weight gain rate (WGR), or the activities of amylase (AMS) and lipase (LPS). Furthermore, compared to the HFD group, the INU group exhibited decreased serum levels of low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) in spotted sea bass; additionally, there was a reduction in hepatic T-CHO and LDL-C content. Notably, both the HFD and INU groups demonstrated significantly elevated serum concentrations of total protein (TP) and albumin (ALB) compared to the CK group. Compared with the HFD group, the INU group demonstrated a reduction in liver fat vacuoles and ballooning degeneration, as well as a decrease in lipid droplet content in spotted sea bass. Additionally, INU (1 %,1.5 %,2 %) significantly downregulated the expression of ACC-2 genes, and significantly upregulated the expression of AMPKα genes. These results suggest that INU has the potential to regulate lipid metabolic disorders induced by HFD.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"605 ","pages":"Article 742510"},"PeriodicalIF":3.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of a cell line from Yunlong grouper (Epinephelus moara ♀ and Epinephelus lanceolatus ♂) larva and its response to infectious spleen and kidney necrosis virus infection","authors":"Chenfei Guo ,&nbsp;Zhihong Gong ,&nbsp;Fei Fang ,&nbsp;Qi Zhang ,&nbsp;Na Wang ,&nbsp;Yuqin Su ,&nbsp;Lei Wang ,&nbsp;Zhenxia Sha ,&nbsp;Songlin Chen","doi":"10.1016/j.aquaculture.2025.742476","DOIUrl":"10.1016/j.aquaculture.2025.742476","url":null,"abstract":"<div><div>The Yunlong grouper is a novel hybrid species resulting from the crossbreeding of <em>Epinephelus moara</em> ♀ and <em>Epinephelus lanceolatus</em> ♂. This hybrid species exhibits a combination of desirable traits inherited from both parent species. This study established the first Yunlong grouper cell line from larvae, named Glar (Grouper larvae).</div><div>The cells were cultured in L-15 medium containing 15 % serum in 24 °C and have culture more than 80 passages. Chromosome karyotype analysis showed that the chromosome number was 2n = 48, and transcriptome analysis indicated that the cell line is mainly composed of fibroblast. After cryopreservation in liquid nitrogen for 6 months, cell viability remained above 70 %. The cells can be transfected, as fluorescence was observed after transfection with both Cy3-labeled scramble siRNA and the pEGFP-N1 plasmid. Furthermore, after infection with two iridoviruses, spotted knifejaw iridovirus (SKIV-SD) and spotted seabass iridovirus (SBIV-V12), Glar cells showed significant cytopathic effect (CPE), and viral particles were observed using transmission electron microscopy. Transcriptome analysis revealed that five days post-infection with SBIV-V12, differentially expressed genes were primarily enriched in pathways related to cell replication, the cell cycle, DNA repair, the p53 signaling pathway, and the Fanconi anaemia pathway, with 20 of 21 genes in the p53 pathway showing upregulation. The p53 signaling pathway plays a crucial role in antiviral immunity. Therefore, this cell line can serve as a valuable tool for in vitro research on the prevention and control of iridovirus, as well as for studying the disease mechanisms and potential therapeutic strategies for the Yunlong grouper.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742476"},"PeriodicalIF":3.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}