Low-dose astaxanthin promotes growth via bile acid-regulated metabolic and lipid-antioxidant pathways in Scylla paramamosain

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-20 DOI:10.1016/j.aquaculture.2025.743220

Yao Deng , Shichao Xie , Wenhao Zhan , Tingting Zhu , Hongyu Peng , Haiqing Cao , Zheng Tang , Xiaoyue Li , Tiantian Xu , Min Jin , Qicun Zhou

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引用次数: 0

Abstract

This study evaluated the synergistic effects of astaxanthin (AST) and bile acids (BA) on growth, lipid metabolism, and antioxidant capacity in Scylla paramamosain through a 63-day feeding trial using a total of 150 crabs (30 crabs per group) integrated with multi-omics analysis (initial weight: 9.07 ± 0.05 g). Five experimental diets were formulated: control (SA0), 50 mg kg⁻¹ AST (SA50), 50 mg kg⁻¹ AST with 0.02 % BA (SA50 + BA2), 50 mg kg⁻¹ AST with 0.04 % BA (SA50 + BA4), and 100 mg kg⁻¹ AST (SA100), respectively. The results indicated that crabs fed diets with SA50 + BA2 and SA100 exhibited higher percent weight gain (PWG) and specific growth rate (SGR) than those fed the other diets (p < 0.05). Both SA50 + BA2 and SA100 increased hemolymph triglycerides (TG) but reduced hepatic triglyceride accumulation by enhancing carnitine palmitoyltransferase 1 activity (p < 0.05). Moreover, crabs fed diets with SA50 + BA2 and SA100 showed higher activities of Cu/Zn-superoxide dismutase (Cu/Zn-SOD) and glutathione peroxidase (GPx) (p < 0.05), while reducing concentration of malondialdehyde (MDA) and activity of alanine aminotransferase (p < 0.05). Multi-omics analysis revealed the synergistic interaction of BA-AST through methyl and metabolic coupling: BA inhibited the expression of chitin hydrolysis gene (chitinase) through methyl donor optimization, up-regulated the expression of 5-methylcytosine and down-regulated expression of gnmt (p < 0.05). Simultaneously, AST inhibited phospholipid synthesis (slc5a7 downregulation and decreased PC (P-18:1 (9Z) / 0:0), p < 0.05), while BA activated carnitine shuttling (bbox1 upregulation and carnitine elevation, p < 0.05), jointly mitigating hepatic lipidosis. AST-mediated ROS scavenging (sod1 upregulation) synergized with BA-induced sulfur metabolism (cbs) and iron regulation (fth1) to enhance redox homeostasis. Pathway analysis confirmed mutual enhancement between AST and BA, with BA improving AST absorption and AST promoting BA-induced β-oxidation. This nutrient synergy enabled a 50 % reduction in AST dosage without compromising growth or antioxidant efficacy, primarily via lipid redistribution and methyl-metabolic coupling.

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在Scylla paramamosain中，低剂量虾青素通过胆汁酸调节的代谢和脂质抗氧化途径促进生长

本研究采用初始体重为9.07±0.05 g的150只大闸蟹（每组30只），通过多组学分析，研究虾青素（AST）和胆汁酸（BA）对Scylla paramamosain生长、脂质代谢和抗氧化能力的协同效应。配制5种试验饲粮，分别为对照（SA0）、50 mg kg−1 AST （SA50）、50 mg kg−1 AST (0.02% BA) （SA50 + BA2）、50 mg kg−1 AST (0.04% BA) （SA50 + BA4）和100 mg kg−1 AST （SA100）。结果表明，饲料中添加SA50 + BA2和SA100的螃蟹增重率和特定生长率均高于其他饲料（p < 0.05）。SA50 + BA2和SA100均增加血淋巴甘油三酯（TG），但通过增强肉毒碱棕榈酰基转移酶1活性降低肝脏甘油三酯积累（p < 0.05）。此外，SA50 + BA2和SA100均提高了铜锌超氧化物歧化酶（Cu/Zn-SOD）和谷胱甘肽过氧化物酶（GPx）活性（p < 0.05），降低了丙二醛（MDA）浓度和丙氨酸转氨酶活性（p < 0.05）。多组学分析揭示了BA- ast通过甲基和代谢耦合的协同相互作用：BA通过甲基供体优化抑制几丁质水解基因（几丁质酶）的表达，上调5-甲基胞嘧啶的表达，下调gnmt的表达（p < 0.05）。同时，AST抑制磷脂合成（slc5a7下调，降低PC (p -18:1 (9Z) / 0:0), p < 0.05）， BA激活肉毒碱穿梭（bbox1上调，肉毒碱升高，p < 0.05），共同缓解肝脂质沉积。ast介导的ROS清除（sod1上调）与ba诱导的硫代谢（cbs）和铁调节（fth1）协同，增强氧化还原稳态。途径分析证实AST与BA相互增强，BA促进AST吸收，AST促进BA诱导的β-氧化。这种营养协同作用使AST用量减少50%，而不影响生长或抗氧化效果，主要通过脂质再分配和甲基代谢偶联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.