Isolation and characterization of novel Yersinia ruckeri bacteriophages for potential use in aquaculture

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-21 DOI:10.1016/j.aquaculture.2025.743219

Ilhan Altinok , Mustafa Ture , Dilek Ustaoglu , Ayse Cebeci , Rafet Çağrı Öztürk , Elif Aygür , Özcan Kaygusuz

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Abstract

Yersiniosis, caused by Yersinia ruckeri, poses a major threat to aquaculture, particularly in rainbow trout (Oncorhynchus mykiss). This study aimed to isolate, characterize, and evaluate the therapeutic potential of two novel Y. ruckeri-specific bacteriophages, named YP3 and YP10S2S2. Their genomes consisted of double-stranded DNA (dsDNA), with sizes of 36,990 bp for YP3 and 56,579 bp for YP10S2. Phylogenomic analysis classified YP10S2 within the family Drexlerviridae, though its genus and species remain unidentified, while YP3 was placed in the class Caudoviricetes but lacked further taxonomic resolution. Thermal and pH stability tests indicated optimal viability at temperatures up to 25 °C and pH 4–10. in vitro assays demonstrated high adsorption efficiency, short latent periods, and strong lytic activity. Cytotoxicity assays on EPC cells revealed no adverse effects, confirming phage safety. Fish challenge experiment revealed that oral administration of a phage-supplemented diet provided 70 % protection, outperforming intraperitoneal injection (56 %), highlighting oral phage therapy as a more effective disease control strategy. These results suggest that YP3 and YP10S2 are promising candidates for phage therapy in aquaculture due to their specificity, stability, and effectiveness against Y. ruckeri, offering a viable alternative to antibiotics for treating bacterial diseases in fish.

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用于水产养殖的新型拉克氏耶尔森菌噬菌体的分离和鉴定

由拉克氏耶尔森氏菌引起的耶尔森氏菌病对水产养殖，特别是虹鳟（Oncorhynchus mykiss）造成重大威胁。本研究旨在分离、表征和评估两种新型ruckeri特异性噬菌体YP3和YP10S2S2的治疗潜力。它们的基因组由双链DNA （dsDNA）组成，YP3的大小为36,990 bp， YP10S2的大小为56,579 bp。系统基因组学分析将YP10S2归为Drexlerviridae科，尽管其属和种尚未确定，而YP3被置于Caudoviricetes纲，但缺乏进一步的分类解析。热稳定性和pH稳定性试验表明，温度为25°C， pH为4-10时，活性最佳。体外实验表明其吸附效率高，潜伏期短，裂解活性强。对EPC细胞的细胞毒性试验显示无不良反应，证实了噬菌体的安全性。鱼类挑战实验显示，口服噬菌体补充饲料提供70%的保护，优于腹腔注射（56%），强调口服噬菌体治疗是一种更有效的疾病控制策略。这些结果表明，YP3和YP10S2因其特异性、稳定性和对拉克氏菌的有效性而成为水产养殖中噬菌体治疗的有希望的候选药物，为治疗鱼类细菌性疾病提供了可行的替代抗生素。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.