Aquaculture最新文献

{"title":"Double-stranded RNA delivery through soaking stimulates antiviral responses in shrimp","authors":"Nipatthra Phromma-in ,&nbsp;Kamonwan Maruekawong ,&nbsp;Pongsopee Attasart","doi":"10.1016/j.aquaculture.2025.742681","DOIUrl":"10.1016/j.aquaculture.2025.742681","url":null,"abstract":"<div><div>Shrimp rely on innate immunity to defend against viral infections. Recently, dsRNA-mediated technology has emerged as a promising tool for virus control in shrimp. While injection and feeding methods effectively inhibit viral replication, they are unsuitable for post-larval shrimp. This study evaluates the potential of soaking shrimp in dsRNA solution to activate RNA interference (RNAi) and enhance antiviral immunity against White Spot Syndrome Virus (WSSV). Shrimp were soaked in dsRNA targeting clathrin heavy chain (dsCHC) or green fluorescent protein (dsGFP) for 3 h. RNAi-related genes (Sid1, Dicer2, Ago2) were upregulated, although specific mRNA silencing was not detected. The induction of these components significantly enhanced RNAi efficacy. Additionally, non-specific antiviral gene, like IRF, was elevated. When pre-soaked shrimp were challenged with WSSV, virus levels in the gills decreased by 87 % in the low-dose group, and cumulative mortality reduced from 47 % in the control group to 16.6 %. In the high-dose group, mortality decreased significantly within the first two days, despite all shrimp succumbing by day 7. The results suggest that while specific mRNA silencing by dsRNA soaking was not observed, the dsRNA was capable of activating shrimp antiviral responses, including RNAi and non-specific immunity, leading to a significant reduction in virus levels and shrimp mortality from WSSV infection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742681"},"PeriodicalIF":3.9,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fate and health implications of polycyclic aromatic hydrocarbons in a sustainable food waste recycling system using housefly maggots and Nile tilapia","authors":"Chao Zheng ,&nbsp;Zhang Cheng ,&nbsp;Zhi-Lin Chen ,&nbsp;Yu Bon Man ,&nbsp;Qiao Liu ,&nbsp;Liu-Lan Zhao ,&nbsp;Song Yang ,&nbsp;Ming Hung Wong","doi":"10.1016/j.aquaculture.2025.742675","DOIUrl":"10.1016/j.aquaculture.2025.742675","url":null,"abstract":"<div><div>This study aimed to investigate the transformation, bioaccumulation, and human and ecological health risks of polycyclic aromatic hydrocarbons (PAHs) in a food waste culture system that included housefly maggots (<em>Musca domestica</em>) and Nile tilapia (<em>Oreochromis niloticus</em>). Feeding trials were carried out in 25 groups of maggots under different breeding densities (2.50, 5.00, 7.50, 10.0, and 12.5 g/kg), using a well-mixed culture medium with measured proportions of rice waste (RW) and meat and vegetable waste after pretreatment (A-MVW) (rinsing and draining). The ratio of RW to A-MVW was 100:0, 75:25, 50:50, 25:75, and 0:100 %. The results showed that the ∑PAHs contents in housefly maggots fed with food waste ranged 22.5–232 μg/kg ww, with the percentage of high molecular weight PAHs being 86.2 ± 12.4 %, with carcinogenic PAHs accounting for 24.3–93.5 %. Based on the PAHs content and growth indexes of the maggots, the optimal conditions were a breeding density of 10.0–12.5 g/kg and food waste substrate of 75 %–100 % A-MVW. The lowest PAHs content was 22.5–28.4 μg/kg, the highest yield 136–172 g/kg, the highest content of crude protein 49.2 %–56.6 %, and the highest content of crude lipid 19.8 %–20.7 %. Concentrations of ∑PAHs (20.3 ± 5.30 μg/kg ww) in residues from waste converted by housefly maggots were not significantly different among different treatment groups (<em>p</em> &gt; 0.05). It was determined that there is no potential ecological risk from PAHs if the residues are utilized as organic fertilizers in agricultural production. There was no significant difference between the concentration of PAHs (12.3 ± 1.78 μg/kg ww) in the tilapia fed with the three maggot feeds and the control diet (<em>p</em> &gt; 0.05). The human health risk assessment showed that the PAHs of tilapia fed housefly maggots did not pose a health risk to consumers. Therefore, housefly maggots cultured from food waste can be used as high-quality and safe protein feed sources for tilapia cultivation.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742675"},"PeriodicalIF":3.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification effect of algae and immobilized bacteria combination system in aquaculture wastewater treatment with targeted removal of pathogenic Vibrio and antibiotic resistance genes","authors":"Na Sun ,&nbsp;Dongfei Xing ,&nbsp;Fang Liu ,&nbsp;Fang Liang ,&nbsp;Lifen Liu","doi":"10.1016/j.aquaculture.2025.742673","DOIUrl":"10.1016/j.aquaculture.2025.742673","url":null,"abstract":"&lt;div&gt;&lt;div&gt;With development of the mariculture industry and increasing seafood demand, mariculture has become an intensive, high-density, and high-yield farming model. The resulting high nitrogen and phosphorus loads pose serious threats to the environment and economy. This study developed an innovative bacteria-algae symbiotic system (BI+A) combining functional nitrogen-removing bacteria isolated from Penaeus albus culture ponds with Tetraselmis subcordiformis to address these critical issues. The bio-system demonstrated exceptional remediation capabilities for &lt;em&gt;Litopenaeus vannamei&lt;/em&gt; tail water treatment, achieving &gt;93 % ammonium removal and 67–95 % reduction of key nutrients including total nitrogen and phosphorus within 4 days. Notably, the technology effectively mitigated biological risks through 97–99 % elimination of pathogenic &lt;em&gt;Vibrio parahaemolyticus&lt;/em&gt; and &lt;em&gt;Metschnikowia bicuspidata&lt;/em&gt;, while significantly reducing total antibiotic resistance genes (ARGs) abundance by 57.9 % with complete removal of critical β-lactam resistance determinants. The integrated bio-remediation approach provide an effective solution for the treatment of mariculture tail water, which helps to solve the problems of environmental pollution and ecological damage faced by the aquaculture industry. It is of great significance for promoting the sustainable development of the mariculture industry.&lt;/div&gt;&lt;div&gt;With development of the mariculture industry and increasing seafood demand, mariculture has become an intensive, high-density, and high-yield farming model. The resulting high nitrogen and phosphorus loads pose serious threats to the environment and economy. Furthermore, numerous microorganisms in tail water, particularly pathogenic bacteria, and antibiotic resistance bacteria and antibiotic resistance genes (ARGs) in the breeding environment and cultured organisms, endanger the surrounding population and marine ecosystems. To effectively treat tail water, this study combined treatment technology based on bacteria and algae, using isolated strains with nitrogen-removal function from a &lt;em&gt;Penaeus albus&lt;/em&gt; culture tail water pond, and cultured the dominant bacteria groups. A semi-fixed bacteria–algal bio-system (BI+A) was then established with &lt;em&gt;Tetraselmis subcordiformis,&lt;/em&gt; and removal rates of nutrients, pathogenic bacteria, and ARGs from &lt;em&gt;Litopenaeus vannamei&lt;/em&gt; tail water were investigated. BI+A efficiently removed nutritive substances in tail water, particularly nitric and total nitrogen, and active and total phosphorus; after 4 d of operation, the NH&lt;sub&gt;4&lt;/sub&gt;&lt;sup&gt;+&lt;/sup&gt; removal efficiency reached 93.45 %. The total nitrogen, active phosphorus, total phosphorus, and chemical oxygen demand removal efficiency reached 67 %, 95 %, 51 %, and 75.2 %, respectively. Pathogenic &lt;em&gt;Vibrio&lt;/em&gt; (&lt;em&gt;Vibrio parahaemolyticus&lt;/em&gt;) and pathogenic yeast (&lt;em&gt;Metschnikowia bicuspidata&lt;/em&gt;) removal rates were 97 % and 99.1 %, respectively. T","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742673"},"PeriodicalIF":3.9,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterozygote advantage of hybrid varieties from red crucian carp and white crucian carp revealed by genome wide association analysis","authors":"Yun Zeng ,&nbsp;Zhiyi Zhang ,&nbsp;Siyu Zhao ,&nbsp;Jun Wang ,&nbsp;Yi Zhou ,&nbsp;Dong Liu ,&nbsp;Chenghui Wang","doi":"10.1016/j.aquaculture.2025.742672","DOIUrl":"10.1016/j.aquaculture.2025.742672","url":null,"abstract":"<div><div>Distant hybridization in fish reshapes genetic materials of hybrid varieties, thereby providing heterosis in growth process. However, genetic basis of growth advantage remains poorly understood. In this study, we conducted whole-genome resequencing, RNA-seq analyses, and genome-wide association studies (GWAS) on 400 individuals from white crucian carp (<em>Carassius cuvieri</em>, WCC), red crucian carp (<em>Carassius auratus red var</em>., RCC), and their hybrids (WR and WR-II). We identified 14,169,714 filtered single nucleotide polymorphisms (SNPs), with an average SNP density of less than 700 bp across genome. 18 SNPs were significantly associated with growth traits, and 306 genes were annotated in genomic regions around the SNP loci. Lead SNP-associated candidate genes, including SLC35F3, HERC2 and MAP2K2, showed higher expression levels, correlating positively with WR-II's growth advantage. Heterozygous frequencies for WCC and RCC were zero, except for a 20 % frequency in RCC for body depth (BD)-associated lead SNP. Excluding BD-associated SNP, WR-II had higher heterozygous frequencies (24 %–33.3 %) than WR (1.9 %–15.4 %) across four lead SNPs, except for the body length-associated SNP variants (80.8 % for WR and 52.2 % for WR-II). Among individuals with dominant growth, genotype frequencies for two heterozygous SNPs were higher in WR-II (28.6 %) than in WR (21.2 %), and for three heterozygous SNPs, the frequencie was significantly higher in WR-II (14.3 %) compared to WR (1.9 %) (<em>p</em> &lt; 0.05). Notably, WR had no individuals with four heterozygous SNPs, while WR-II had 9.5 %. These results suggest that hybridization increases allelic heterozygosity, contributing to growth advantage by upregulating gene expression. Our findings provide valuable insights into genetic basis of heterosis in hybrid varieties.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742672"},"PeriodicalIF":3.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice-fish co-culture improves carp (Cyprinus carpio) quality by modulating the gut microbiota and metabolites","authors":"Zhijuan Nie ,&nbsp;Xiaotong Xu ,&nbsp;Yanhan Ji ,&nbsp;Ping Gao ,&nbsp;Sihan Zhou ,&nbsp;Yi Sun ,&nbsp;Jian He ,&nbsp;Lanmei Wang ,&nbsp;Nailing Shao ,&nbsp;Yu Shi ,&nbsp;Xu Liu ,&nbsp;Pao Xu ,&nbsp;Baozhan Wang","doi":"10.1016/j.aquaculture.2025.742659","DOIUrl":"10.1016/j.aquaculture.2025.742659","url":null,"abstract":"<div><div>Rice-fish co-culture systems, as compared to fish monoculture, offer a sustainable agriculture model that balances productivity with environmental conservation. While the important role of gut microbiome in fish growth and health is increasingly recognized, the impact of different farming practices on fish quality and their gut microbiome remains insufficiently understood. To address this gap, we conducted a comprehensive survey of 292 carps (<em>Cyprinus carpio</em>) raised in four rice-fish co-culture systems (RF) across China, comparing them with site-matched pond systems (PF). Through a combination of amplicon sequencing, metabolomic analysis, and bioinformatics, we investigated changes in carp quality, as well as alterations in gut microbiota and metabolites, and explored the potential links between them. Our results reveal that RF system significantly improve carp quality and alter gut metabolite profiles. Further analysis of gut microbial subgroup showed that RF co-culture induced homogenization in the community structure of rare microbial taxa. Ecological network analysis indicated that RF enhances the complexity and stability of microbial networks, particularly among rare taxa, which play a previously underestimated role in gut ecosystems. Community assembly analysis suggested that deterministic processes more strongly shape the microbial communities in carps from RF system, promoting habitat-dependent selection. By developing a bi-network model to explore host-microbe interactions, we identified key microbial taxa, such as <em>Cetobacterium</em>, that are closely associated with beneficial traits in carp quality. Overall, this study highlights the potential of Rice-fish co-culture to foster beneficial host-microbe interactions, providing a microbial ecological perspective on understanding aquaculture safety and sustainability.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742659"},"PeriodicalIF":3.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-species single-cell atlas of hemocyte conservation and diversification in crustaceans","authors":"Chenchang Bao,&nbsp;Tao Jing,&nbsp;Yanan Yang","doi":"10.1016/j.aquaculture.2025.742670","DOIUrl":"10.1016/j.aquaculture.2025.742670","url":null,"abstract":"<div><div>Crustacean immunity plays a crucial role in aquaculture disease management, while a systematic comparison of hemocyte heterogeneity, developmental trajectories, and immune response plasticity across ecologically divergent crustacean species remains largely unexplored. This study employed cross-species single-cell RNA sequencing analysis to conduct a comprehensive comparative of hemocytes from four economically important crustacean species: <em>Procambarus clarkii</em> (21,623 cells), <em>Penaeus japonicus</em> (43,054 cells), <em>Penaeus monodon</em> (30,913 cells), and <em>Penaeus vannamei</em> (145,094 cells). Conserved hemocyte populations, including hematopoietic progenitors (prohemocytes) and mature effector cells (e.g., proPO and VEGF/ALF clusters), were identified. Trajectory analysis revealed developmental relationships between hemocyte subsets, with prohemocytes consistently positioned at the start of differentiation pathways across species. Pseudotime analysis revealed two conserved differentiation trajectories: from prohemocytes to proPO lineage (melanization/antimicrobial synthesis) and from prohemocytes to VEGF/ALF lineage (phagocytosis/antimicrobial synthesis). Species-specific variations in cluster composition and gene expression reflect adaptations to distinct ecological niches. Comparative analysis of immune-related genes expression, including prophenoloxidase system components and antimicrobial peptides, uncovered both conserved and species-specific patterns, reflecting evolutionary adaptations to diverse aquatic environments. Differential responses to immune challenges (LPS, WSSV, and ammonia stress) underscore the complexity and adaptability of crustacean immune systems. These findings offer novel insights into crustacean hematopoiesis and immune cell diversity, offering a valuable resource for invertebrate immunity and potential applications in aquaculture disease management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742670"},"PeriodicalIF":3.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary calcium promotes growth, lipid metabolism and energy metabolism by activating the Ca2+-Camkkβ-AMPK signaling pathway in Pacific white shrimp (Litopenaeus vannamei)","authors":"Yu He ,&nbsp;Tiantian Xu ,&nbsp;Min Jin ,&nbsp;Xiaoru Chen ,&nbsp;Yinzhao Zhang ,&nbsp;Feng Tang ,&nbsp;Lu Zhang ,&nbsp;Tingting Zhu ,&nbsp;Yi Wu ,&nbsp;Jinlin Wang ,&nbsp;Shuqin Li ,&nbsp;Qicun Zhou","doi":"10.1016/j.aquaculture.2025.742671","DOIUrl":"10.1016/j.aquaculture.2025.742671","url":null,"abstract":"<div><div>This study aimed to evaluate the effects of different dietary calcium levels on the growth, lipid and energy metabolism, and calcium-mediated Ca²⁺-Camkkβ-AMPK signaling pathway of <em>Litopenaeus vannamei</em>. Six isonitrogenous and isolipidic diets were formulated to contain different calcium levels, the analyzed calcium were 2.12 %, 2.27 %, 2.35 %, 2.47 %, 2.54 %, and 2.66 %, respectively. The results showed that shrimp fed diets with 2.35 % to 2.54 % calcium were significantly higher final weight (FW), percent weight gain (PWG), specific growth rate (SGR) and feed efficiency (FE) than those fed with the control diet (<em>P</em> &lt; 0.05). The highest triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) in the hemolymph, the activities of the rate-limiting enzymes of succinate dehydrogenase (SDH), CA, citric acid (CA), creatine kinase (CK), pyruvate kinase (PK) and energy metabolism-related indicators (ATP, NADH, NAD⁺) in the hepatopancreas mitochondria were observed at shrimp fed diet with 2.47 % calcium (<em>P</em> &lt; 0.05). The expression of genes related to the calcium signaling pathway (<em>pka</em>, <em>cam</em>, <em>pde</em>), molting (<em>mih</em>, <em>ecr</em>, <em>flz-f1</em>, <em>ethr</em>, <em>chs</em>, <em>e75</em>, <em>ef-1α</em>, <em>chi2</em>), energy metabolism (<em>ampkα</em>, <em>ampkβ</em>, <em>ampkγ</em>, <em>acc1</em>, <em>mcd</em>), tricarboxylic acid cycle (<em>sdh</em>, <em>cs</em>, <em>aco</em>, <em>idh</em>, <em>mdh</em>, <em>fh</em>), mitochondrial electron respiratory chain (<em>ndh</em>, <em>cco</em>, <em>cox</em>, <em>atph</em>), and lipid metabolism (<em>fas</em>, <em>srebp</em>) related genes were significantly upregulated at shrimp fed diet with 2.47 % calcium (<em>P</em> &lt; 0.05). In conclusion, dietary calcium level of 2.47 % can promote molting and growth by activating the Ca²⁺-Camkkβ-AMPK signaling pathway, promoting the tricarboxylic acid cycle and energy metabolism, and promoting lipid metabolism of L. <em>vannamei</em>.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742671"},"PeriodicalIF":3.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic adaptations: Unraveling the skin microbiome of bullfrogs (Rana catesbeiana) under bacterial disease stress","authors":"Shan Hu ,&nbsp;Gongshi Lin ,&nbsp;Theerakamol Pengsakul ,&nbsp;Lixing Huang","doi":"10.1016/j.aquaculture.2025.742658","DOIUrl":"10.1016/j.aquaculture.2025.742658","url":null,"abstract":"<div><div>Amphibians transition between aquatic and terrestrial environments throughout their lifecycle, a unique adaptation that fosters intimate interactions between their skin microbiome and the surrounding environment, thereby exhibiting distinctive and diverse microbial ecological characteristics. They are not only indicators of health but also harbingers of disease. This study focuses on bullfrogs (<em>Rana catesbeiana</em>) and comprehensively analyzes the dynamic changes in skin microbiomes under different disease conditions, revealing their crucial impact on host health. This study examines the skin microbiome of bullfrogs infected with three prevalent bacterial diseases (red-leg syndrome, edema syndrome, and meningitis-like infectious disease) in aquaculture settings, comparing them with microbiomes of healthy bullfrogs. Through 16S rDNA sequencing, a total of 1059 amplicon sequence variants (ASVs) were identified, encompassing 27 phyla, 57 classes, 133 orders, 223 families, and 398 genera. Among them, Bacteroidetes, Proteobacteria and Firmicutes are predominant bacterial phyla in the skin of healthy bullfrogs. At the genus level, compared to the healthy group (HS), bullfrogs with edema syndrome (ES) exhibited higher relative abundances of <em>Acinetobacter</em>, <em>Arcobacter</em>, and <em>Aeromonas</em>; bullfrogs with meningitis-like infectious disease (MID) showed higher relative abundances of <em>Acinetobacter</em>, <em>Flavobacterium</em>, <em>Pedobacter</em>, <em>Arcobacter</em>, and <em>Comamonas</em>; and bullfrogs with RLS showed higher relative abundances of <em>Acinetobacter</em>, <em>Acinetobacter</em>, <em>Flavobacterium</em> and <em>Aeromonas</em>. Simultaneously, significant functional changes were observed in the skin microbiota of MID. Compared to healthy bullfrogs, bullfrogs with ES exhibited marked alterations in pathways such as Carbohydrate metabolism, Xenobiotics biodegradation and metabolism, Metabolism of cofactors and vitamins, and Amino acid metabolism. Bullfrogs with MID showed pronounced disruptions in pathways related to energy metabolism, while those with RLS displayed notable dysregulation in Folding, sorting and degradation, Nervous system, and Translation pathways. These results indicate that changes in composition and function of the bullfrog skin microbiome are closely associated with their health status, with significant differences observed among microbiome characteristics under different disease conditions. These variations in the skin microbiome not only serve as important diagnostic markers for bacterial infections but also offer potential intervention strategies for the prevention and control of such diseases. A deeper understanding of these microbial changes' mechanisms could facilitate the development of novel disease management strategies, thereby enhancing the health management practices in bullfrog aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742658"},"PeriodicalIF":3.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary fatty acid composition modulates growth in the marine teleost Trachinotus ovatus by regulating the hepatic triglyceride to (phosphatidylcholine + phosphatidylethanolamine) ratio via the Ampk/Srebp signaling pathway","authors":"Guanrong Zhang,&nbsp;Jianzhao Xu,&nbsp;Fang Chen,&nbsp;Junfeng Guan,&nbsp;Ningning Su,&nbsp;Xin Gao,&nbsp;Dizhi Xie,&nbsp;Yuanyou Li","doi":"10.1016/j.aquaculture.2025.742660","DOIUrl":"10.1016/j.aquaculture.2025.742660","url":null,"abstract":"<div><div>Previous studies have demonstrated superior growth performance in <em>Trachinotus ovatus</em> fed a blended oil 1 (BO1, optimized for essential fatty acid requirements) compared to those receiving blended oil 2 (BO2, fish oil:soybean oil, 2:3). To elucidate the mechanistic basis of dietary fatty acid effects on growth, we conducted lipidomic, biochemical, and transcriptomic analyses using tissue samples from <em>T. ovatus</em> (initial weight: 7.63 ± 0.03 g) fed the BO1 or BO2 diets for 9 weeks. Fish fed BO1 exhibited elevated hepatic phosphatidylcholine (PC) and phosphatidylethanolamine (PE) levels, reduced lipid and triglyceride (TG) content, and lower TG/(PC + PE) ratio (<em>P</em> &lt; 0.05). Serum analyses revealed significantly decreased TG, total cholesterol, and free fatty acid levels in BO1-fed fish (<em>P</em> &lt; 0.05). Transcriptomic profiling demonstrated upregulation of fatty acid synthesis genes (<em>srebp1c</em>, <em>fas</em>, <em>acc1</em>, <em>scd1</em>), PC/PE synthesis genes (<em>pcyt1</em>, <em>pcyt2</em>), and TG catabolism gene (<em>atgl</em>) in the BO1 group, while TG synthesis (<em>dgat</em>) and energy metabolism (<em>ampk</em>) genes were downregulated (<em>P</em> &lt; 0.05). In vitro experiments with <em>T. ovatus</em> hepatocytes showed that Ampk inhibition increased Pcyt1 and Atgl activities, elevated PC levels, and reduced TG/(PC + PE) ratios (<em>P</em> &lt; 0.05). Srebp1c overexpression upregulated PC synthesis genes, increased PC content, and decreased TG/(PC + PE) ratios (<em>P</em> &lt; 0.05). Treatment with BO1- or BO2-mimetic fatty acid blends recapitulated in vivo observations. These findings suggest that BO1's optimized fatty acid composition enhances PC and PE biosynthesis and TG catabolism via the Ampk/Srebp pathway, ultimately resulting in a reduced hepatic TG/(PC + PE) ratio, optimized lipid homeostasis, and enhanced <em>T. ovatus</em> growth.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742660"},"PeriodicalIF":3.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleanolic acid-targeted LAMP1 mediates autophagy and lysosome to alleviate high-fat-induced hepatic lipid deposition","authors":"Kai Yu ,&nbsp;Ruijie Guo ,&nbsp;Delun Huang ,&nbsp;Xuhong Yang ,&nbsp;Dandan Wang ,&nbsp;Kai Huang ,&nbsp;Jianqin Liang","doi":"10.1016/j.aquaculture.2025.742656","DOIUrl":"10.1016/j.aquaculture.2025.742656","url":null,"abstract":"<div><div>Non-alcoholic fatty liver disease (NAFLD) threatens aquaculture worldwide and is closely associated with autophagic disturbance. Activity ingredients from <em>Sedum sarmentosum</em> Bunge (SSB) have been shown to alleviate high-fat diet (HFD)-induced NAFLD in tilapia. However, the precise active ingredients and their involvement in regulating autophagy remain unclear. This study aims to investigate the impact of the special active compounds in SSB responsible for ameliorating NAFLD and to elucidate their molecular mechanisms involving autophagy in hepatocytes. Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was employed to characterize the active ingredients of SSB. The pharmacological effects of oleanolic acid (OA) were evaluated in HFD-induced tilapia through analysis of growth parameters, biochemical indicators, and hepatic pathology. In vitro, the effects of OA combined with rapamycin or chloroquine on the free fatty acids (FFA)-induced HepG2 cells were assessed by measuring lipid accumulation, autophagy, and lipophagy. OA target proteins were identified using an affinity-based probe combined with chemical proteomics. OA notably decreased the hepatosomatic indices, improved liver function, and suppressed lipid deposition in HFD-induced tilapia. Transmission electron microscopy revealed that OA mitigated hepatocellular structural damage and enhanced autophagosome formation. In FFA-induced HepG2 cells, OA treatment enhanced antophagy, autophagic flux and lipophagy, as evidenced by an increased LC3II/I ratio, elevated autolysosome formation, and enhanced colocalization of LC3 with Bodipy. Notably, rapamycin and chloroquine respectively promote and inhibited these effects. Chemical proteomics identified LAMP1 as a direct binding target of OA, which was further validated by the MicroScale thermophoresis (MST) and drug affinity responsive target stability (DARTS) assays. This study identifies OA as a critical active compound from SSB that ameliorates lipid deposition by regulating the autophagy-lysosome pathway via targeting LAMP1, providing a robust foundation for the development of functional feed additives transitioning from traditional herbal extracts to active ingredients in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"607 ","pages":"Article 742656"},"PeriodicalIF":3.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}