Aquaculture最新文献

{"title":"Hormonal regulation of oocyte maturation and ovulation in lambari (Astyanax altiparanae) induced by different pituitary extracts","authors":"Mariana Roza de Abreu ,&nbsp;Laíza Maria de Jesus Silva ,&nbsp;Cristiane Fernanda Benevente ,&nbsp;Roosevelt Passos Barbosa ,&nbsp;Alejandro Sebastian Mechaly ,&nbsp;Sergio Ricardo Batlouni","doi":"10.1016/j.aquaculture.2025.742843","DOIUrl":"10.1016/j.aquaculture.2025.742843","url":null,"abstract":"<div><div>The processes of final oocyte maturation (FOM) and ovulation remain poorly understood in neotropical fish and hypophysation is the most common method to induce these processes in captivity. Therefore, this study aimed to investigate mechanisms controlling FOM and ovulation during hypophysation with carp (CPE) and lambari pituitary extract (LPE), using lambari (<em>Astyanax altiparanae</em>) as a model. A completely randomized experiment was conducted with three treatments, including fractionated doses of 6 mg/kg of CPE or LPE, and a control group injected with saline solution. Females received two intraperitoneal injections and five females <em>per</em> treatment were randomly sampled at six time points to assess plasma DHP and PGF_2α levels, ovarian gene expression and histology. Both CPE and LPE reduced <em>lhr</em> expression while increasing <em>hsd20b2</em> and <em>ptger4b</em> expression and plasma DHP levels, ultimately leading to FOM and ovulation. This suggests that Lh receptor activation in follicular cells triggers <em>hsd20b2</em> synthesis<em>,</em> promoting DHP production and release. In contrast, no significant molecular and hormonal changes were observed in the control, which exhibited low levels of germinal vesicle breakdown (GVBD) and post-ovulatory follicles (POF). A positive correlation among DHP levels, GVBD, and POF further underscored their association with FOM and ovulation. Overall, hypophysation with either CPE or LPE effectively induces FOM and ovulation, thereby enhancing productivity in lambari. However, our findings indicate that LPE achieves greater synchronization of FOM and ovulation events across females. Additionally, as a locally sourced product, LPE may present a more economically viable option for Brazilian aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742843"},"PeriodicalIF":3.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MAPK/ERK-driven transcriptional regulation mediates divergent thermal tolerance in two congeneric oyster species","authors":"Min Wang ,&nbsp;Chaogang Wang ,&nbsp;Mingyang Du ,&nbsp;Zhuxiang Jiang ,&nbsp;Jincheng Chen ,&nbsp;Rihao Cong ,&nbsp;Wei Wang ,&nbsp;Guofan Zhang ,&nbsp;Li Li","doi":"10.1016/j.aquaculture.2025.742855","DOIUrl":"10.1016/j.aquaculture.2025.742855","url":null,"abstract":"<div><div>Oysters, globally distributed filter-feeding bivalves, are ecologically and economically vital, yet summer mass mortality events due to global warming threaten their sustainable development. ERK1/2 function as a phosphorylation-activated molecular switch in MAPK pathway, transducing thermal stress signals via nuclear translocation and transcription factor interaction to regulate thermotolerance-related genes expression in oysters. In this study, we comprehensively investigated the transcriptional regulatory role of ERK kinase in oyster thermotolerance through combined treatment with ERK kinase inhibitors and heat stress, integrated with RNA-Seq and physiological/biochemical assays. Our data demonstrate that ERK, under heat stress, activated phagocytosis (<em>Drpr</em>, <em>Sqstm1</em>), coordinated intricate pathway cross-talk among PI3K-AKT pathway, mTOR pathway, and NF-ĸB signaling pathway (<em>Fos</em>, <em>Trpm2</em>, <em>Pik3ca</em>), inhibited apoptosis (<em>Birc3</em>, Bax, Bcl-2), and optimizing energy conversion processes (HK, PK, <em>C1qtnf3</em>, <em>Fasn</em>). The integrated transcriptomic analysis of <em>Crassostrea gigas</em> and <em>Crassostrea angulata</em> further confirmed the critical role of ERK-regulated genes in thermal adaptation. This study elucidates the transcriptional regulation network of MAPK/ERK pathway in oyster thermotolerance, and advancing our mechanistic understanding of heat resilience in marine invertebrates. Meanwhile, the identified molecular regulators provide actionable targets for breeding heat-resistant oyster strains, offering a dual foundation for both ecological conservation and sustainable oyster aquaculture in the context of global warming.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742855"},"PeriodicalIF":3.9,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms and therapeutic application of pyrogallic acid in treating multidrug-resistant Klebsiella pneumoniae infections in American bullfrogs (Aquarana catesbeiana)","authors":"Han Lin ,&nbsp;Furong Li ,&nbsp;Yufang Huang ,&nbsp;Yongping Ye ,&nbsp;Ziyan Chen ,&nbsp;Chun Liu ,&nbsp;Ruiai Chen","doi":"10.1016/j.aquaculture.2025.742853","DOIUrl":"10.1016/j.aquaculture.2025.742853","url":null,"abstract":"<div><div>Multidrug-resistant <em>Klebsiella pneumoniae</em> (MDR-KP) is listed by the World Health Organization as a critical public-health threat urgently requiring novel antibiotics and has inflicted substantial losses on bullfrog aquaculture. Effective treatment options for infected bullfrogs are currently lacking. In this study, pyrogallic acid (PA) was identified via antibacterial activity screening against MDR-KP and subsequently investigated for its mechanistic effects. In vitro assays revealed concentration-dependent antibacterial activity of PA against a bullfrog-derived MDR-KP strain (NW202109), with both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined as 320 μg/mL, reducing counts ≥ 3 log within 18 h. Molecular docking simulations predicted strong interactions between PA and key residues of MDR-KP proteins, including serine 135 of the AcrB efflux pump, aspartic acid 114 of the OmpK36 porin, and an unidentified residue (UNK276) of ATPase, each stabilized by three hydrogen bonds, suggesting multi-target inhibitory potential. Further analyses confirmed that 4-h PA treatment at MIC significantly increased intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels (<em>P &lt; 0.0001</em>), reduced ATP content by 75.5 %, and inhibited biofilm formation (<em>P &lt; 0.01</em>). In vivo, PA administration (320 μg/mL for 72 h) alleviated pathological damage in the liver, spleen, and kidneys of infected bullfrogs, reduced bacterial loads by 2.74–5.96 log₁₀ CFU/g, and lowered pro-inflammatory cytokine levels. Safety assessments indicated no adverse effects on hematological or hepatic parameters at therapeutic doses.These findings provide preliminary evidence for PA's efficacy against MDR-KP through multi-target mechanisms, establishing a foundation for its application in aquaculture disease control and future therapeutic development.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742853"},"PeriodicalIF":3.9,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different responses of the TGF-β superfamily genes in the warm-water noble scallop Chlamys nobilis to acute and chronic low temperature stress","authors":"Shitong Liu ,&nbsp;Dewei Cheng ,&nbsp;Meizhen Chen ,&nbsp;Huiqi Li ,&nbsp;Naiqian Zhao ,&nbsp;Yaoting Liu ,&nbsp;Beiyu Yan ,&nbsp;Weili Wang ,&nbsp;Hongkuan Zhang ,&nbsp;Huaiping Zheng","doi":"10.1016/j.aquaculture.2025.742852","DOIUrl":"10.1016/j.aquaculture.2025.742852","url":null,"abstract":"<div><div>The Transforming Growth Factor-β (TGF-β) superfamily is comprised of proteins with diverse biological activities, playing a crucial role in immunity, nevertheless few studies have explored their functions in aquatic animals. In this study, 560 <em>TGF-β</em> superfamily genes were first identified through whole-genome analysis from 33 bivalve species, categorized into 10 clusters. Among these<em>,</em> 13 <em>CnTGFB</em> genes were identified in the noble scallop <em>Chlamys nobilis</em>, an important cultured warm-water bivalve in China, were grouped into 9 clusters. The analysis of tissue-specific expression revealed that compared with the testis, the mantle and the adductor muscle, the expression level of the <em>CnTGFB</em> gene was higher in the ovary, the gills, the kidneys and the blood. Under the acute low temperature stress (temperature reduced from 23 °C to 9 °C within 24 h), the expression levels of <em>CnTGFB-02</em>, <em>CnTGFB-07</em>, and <em>CnTGFB-08</em> were significantly increased, while <em>CnTGFB-01a</em>, <em>CnTGFB-01b</em>, <em>CnTGFB-03a</em>, and <em>CnTGFB-05b</em> were significantly decreased. Under the chronic low temperature stress (gradual reduction from 23 °C to 9 °C over 30 days) induced distinct expression patterns: <em>CnTGFB-01a</em>, <em>CnTGFB-02</em>, <em>CnTGFB-03a</em>, <em>CnTGFB-05a</em>, <em>CnTGFB-07</em>, <em>CnTGFB-08</em> and <em>CnTGFB-10</em> showed a significant up-regulation when the temperature declined at 10 °C (<em>P</em> &lt; 0.05), whereas <em>CnTGFB-01b</em>, <em>CnTGFB-04</em>, <em>CnTGFB-05b</em> and <em>CnTGFB-09</em> exhibited significantly down-regulation when the temperature declined at 17 °C (<em>P</em> &lt; 0.05). Moreover, only <em>CnTGFB-05b</em> had significantly higher expression under acute low temperature stress, while 9 genes were more responsive to chronic stress at 9 °C (<em>P</em> &lt; 0.05), and <em>CnTGFB-02</em> had no significantly difference expression at 9 °C (<em>P</em> &gt; 0.05). These results indicated that <em>TGF-β</em> genes play important roles under low temperature stress in the noble scallop, which are also useful for breeding low temperature tolerant varieties in warm-water bivalves.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742852"},"PeriodicalIF":3.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the epigenetic signatures: DNA methylation patterns in the social hierarchies of male giant freshwater prawns (Macrobrachium rosenbergii)","authors":"Liping Li ,&nbsp;Jiongying Yu ,&nbsp;Zhenglong Xia ,&nbsp;Quanxin Gao ,&nbsp;Qiongying Tang ,&nbsp;Shaokui Yi","doi":"10.1016/j.aquaculture.2025.742838","DOIUrl":"10.1016/j.aquaculture.2025.742838","url":null,"abstract":"<div><div>DNA methylation is a cornerstone of epigenetic regulation, yet its role in shaping social hierarchies within crustacean species remains poorly characterized. This study investigated the giant freshwater prawn (<em>Macrobrachium rosenbergii</em>, GFP), which exhibits distinct male morphotypes (blue claws [BC], orange claws [OC], and small males [SM]) and offers a compelling model for studying epigenetic regulation of phenotypic plasticity. Our population analysis revealed a morphotype distribution of 21.37 % BC, 63.36 % OC, and 15.27 % SM. Through comprehensive whole-genome bisulfite sequencing (WGBS) analysis, we identified 211 differentially methylated regions (DMRs) across morphotype comparisons (BC vs. OC, BC vs. SM, OC vs. SM), comprising 77 hypermethylated and 136 hypomethylated regions. These DMRs were functionally associated with 150 differentially methylated genes (DMGs), including 60 hypermethylated and 99 hypomethylated. Notably, hypomethylation of the <em>vg</em> (vitellogenin) 5′ untranslated region in OC individuals enhanced reproductive responsiveness. Additionally, the hypomethylated DMG <em>hspg2</em> (heparan sulfate proteoglycan 2) exhibited significant upregulation in BC group, potentially conferring a selective advantage in limb regeneration—thereby reinforcing social dominance behaviors. Moreover, hypomethylation and concurrent upregulation of the mitochondrial <em>idh2</em> ([NADP]-dependent isocitrate dehydrogenase 2) in SM individuals may underpin a specialized reproductive strategy by optimizing energy metabolism. This study provides insights into the intricate interplay between epigenetic modifications—particularly DNA methylation—and male GFP morphotype diversification. These findings also advance our understanding of epigenetic contributions to phenotypic variation in non-model arthropods and establish a foundation for future research on transgenerational epigenetics in aquatic species.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742838"},"PeriodicalIF":3.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bivalent vaccination with Edwardsiella tarda and Aeromonas salmonicida protected freshwater fish after challenge and consists a strategic approach against pathogens","authors":"Simei He,&nbsp;Guofan Zhang,&nbsp;Yuyuan Peng,&nbsp;Kunyan Li,&nbsp;Jiajie Xie,&nbsp;Ke Zhang,&nbsp;Wen Fu,&nbsp;Yamei Xiao,&nbsp;Liqiu Xia,&nbsp;Shengbiao Hu","doi":"10.1016/j.aquaculture.2025.742834","DOIUrl":"10.1016/j.aquaculture.2025.742834","url":null,"abstract":"<div><div>The delivery of antigens into host cells via the type III secretion system (T3SS) represents a highly effective strategy employed by bacteria to elicit host immune responses and protect against bacterial infections. In this study, we engineered recombinant strains by heterologously biosynthesizing the T3SS from <em>Photorhabdus luminescens</em> TT01 in the avirulent <em>Aeromonas salmonicida</em> DBFF01 strain using tilapia and carp as model. The GAPDH from <em>Edwardsiella tarda</em> was N-terminally fused with the translocation signal of the type III effector LopT and co-expressed with its chaperone, SlcT, in T3SS-expressing <em>A. salmonicida</em> DBFF01, thereby constructing a bivalent vaccine targeting both <em>E. tarda</em> and <em>A. salmonicida</em>. The detection of GAPDH delivery in eukaryotic cells indicates that the heterologous biosynthetic T3SS in <em>A. salmonicida</em> DBFF01 possesses functional activity and can effectively transport GAPDH to carp epithelial cells (EPCs). In vivo trials revealed that tilapia immunized with the bivalent vaccine achieved 100 % relative percentage survival rate when challenged with lethal <em>E. tarda</em> EIB202. Furthermore, the recombinant strains effectively colonized fish tissues without inducing pathology. The vaccination significantly enhanced innate immune enzyme activities and elevated serum IgM levels as well as proinflammatory cytokines (IL-6, TNF-α, IFN-α), and antigen-presenting cell (APC) activation. These findings establish T3SS as a versatile and potent tool for vaccine delivery in aquaculture, combining high efficacy, safety, and robust immune activation to combat bacterial pathogens.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742834"},"PeriodicalIF":3.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and immunological biomarkers of chronic thermal stress in post-smolt Atlantic salmon, Salmo salar","authors":"Barbara Nuic ,&nbsp;Chloe English ,&nbsp;Alyssa Bowden ,&nbsp;Nicholas M. Wade ,&nbsp;Lambertus Koster ,&nbsp;Minami Kawasaki ,&nbsp;Ashleah Williams ,&nbsp;Omar Mendoza-Porras ,&nbsp;Andrew C. Barnes ,&nbsp;Craig E. Franklin ,&nbsp;Richard Smullen ,&nbsp;Rebecca L. Cramp","doi":"10.1016/j.aquaculture.2025.742850","DOIUrl":"10.1016/j.aquaculture.2025.742850","url":null,"abstract":"<div><div>The increasing prevalence of marine heat waves is a challenge facing global aquaculture, especially for temperate species like Atlantic salmon which, in Australia, are farmed in relatively warm waters. However, we do not understand the mechanistic basis for fish underperformance at temperatures above their thermal optimum and this limits our capacity to develop interventions to offset the effects of warming on production efficiencies. In this study, we compared a diverse suite of physiological and immunological biomarkers in post-smolt Tasmanian Atlantic salmon (∼60–100 g) following 6 weeks of exposure to elevated (19 °C) or optimal rearing temperatures (15 °C). Fish held at 19 °C experienced a significant increase in standard metabolic rate, but no change in maximum metabolic rate which resulted in a significant reduction in factorial aerobic scope. There was a significant increase in upper thermal tolerance limits, but a reduction in hypoxia tolerance in 19 °C-exposed fish. CTmax was also more oxygen-dependent in 19 °C fish suggesting that hypoxia can compound the effects of heat stress on Atlantic salmon. Fish held at 19 °C experienced osmoregulatory disturbances with increase in plasma osmotic pressure, but this was not due to reduced performance of branchial Na⁺K⁺ATPase activity. Innate (respiratory burst and white blood cell counts) and adaptive immune responses were also impaired in 19 °C-acclimated salmon. These data demonstrate that the loss of production efficiency in salmon at high temperatures is complex given that multiple physiological systems are impacted simultaneously. Whole animal performance could be improved through the provision of targeted nutritional support to key physiological and/or immune parameters that may be disrupted during periods of thermal stress.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742850"},"PeriodicalIF":3.9,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic infection of the foot of the mediterranean mussel (Mytilus galloprovincialis) by a trematode parasite (Digenea: Monorchiidae) in the farms of Tyrrhenum and Adriatic Sea (Italy)","authors":"Francesca Carella","doi":"10.1016/j.aquaculture.2025.742829","DOIUrl":"10.1016/j.aquaculture.2025.742829","url":null,"abstract":"<div><div>Phases of development of a trematode parasite were observed in the histological samples at foot level of the Mediterranean mussel <em>M. galloprovincialis</em> during routine studies between 2008-2021 along Campania coast. Macroscopically, affected animals may display nodular areas and discolouration at foot level. Histopathology revealed the presence of cercaria and metacercariae present in variable number, from 1 to 6 per foot, variously distributed either at the level of the bixogenic glands, the collagen gland or the adhesive plate, or in the muscular portion, with a prevalence in the affected areas ranging from 3.3 % to 20 %. The invasion appeared to originate at the level of the pedal blood vessels located along the structure of the foot or in the terminal part, up to reaching the various muscle or glandular sites. Molecular assay of the rRNA 18S showed an identity of 96.1 % with a trematode belonging to the <em>Monorchidae</em> familiy named <em>Opistomonorcheides delicatus</em>, commonly infecting Carangidae fishes in Pacific Ocean. This is the first morphological and molecular description of this genus affecting the foot of the mediterranean mussels in Italian waters. The presence of this parasite could affect the mussel thread strength to the farm ropes and help the predation of the succeeding host.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742829"},"PeriodicalIF":3.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning approach for genetic selection of stress response in the Amazon fish Colossoma macropomum","authors":"Celma G. Lemos ,&nbsp;Baltasar F. Garcia ,&nbsp;Marcelo S.S. Filho ,&nbsp;Jairo R. Arango ,&nbsp;Arno J. Butzge ,&nbsp;Luciana Shiotsuki ,&nbsp;Luiz Eduardo L. Freitas ,&nbsp;Fabrício P. Rezende ,&nbsp;Elisabeth C. Urbinati ,&nbsp;Guilherme J.M. Rosa ,&nbsp;Diogo T. Hashimoto","doi":"10.1016/j.aquaculture.2025.742848","DOIUrl":"10.1016/j.aquaculture.2025.742848","url":null,"abstract":"<div><div>This study examined skin color variation in tambaqui (<em>Colossoma macropomum</em>) in response to stress, focusing on morphological and physiological color change mechanisms. A computer vision system (CVS) based on the DeepLab V3 model with ResNet-50 was developed to automate countershading intensity detection. Images from 3780 fish across two populations were used to train a model and estimate genetic parameters for countershading intensity. Morphological color changes were induced in confinement tanks, with countershading intensity observed after 10 days. Physiologically, the α-MSH hormone expanded melanophores by 80 %, intensifying countershading. The CVS achieved high accuracy (88.2 %) for large-scale phenotyping, with moderate to high heritability estimates for color phenotypes: 0.456 ± 0.122 for black pixel percentage, 0.494 ± 0.128 for mean pixel intensity, and 0.192 ± 0.059 for the number of pixels. Low correlations with growth traits suggest that countershading selection can occur without affecting growth, highlighting its potential in breeding programs to improve appearance and stress resilience.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742848"},"PeriodicalIF":3.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of floating bed plants on remediation of eutrophic ponds and cultured species of Cyprinus carpio","authors":"Longjie Li ,&nbsp;Mengqi He ,&nbsp;Yingying Ge ,&nbsp;Wanyu Wei ,&nbsp;Minmin Wu ,&nbsp;Yuanpu Sha ,&nbsp;Shuwen Zhang ,&nbsp;Jing Dong ,&nbsp;Xuejun Li ,&nbsp;Xiaofei Gao ,&nbsp;Huatao Yuan","doi":"10.1016/j.aquaculture.2025.742844","DOIUrl":"10.1016/j.aquaculture.2025.742844","url":null,"abstract":"<div><div>In recent decades, cyanobacterial blooms have emerged as a major constraint on the sustainable development of freshwater aquaculture. Due to their capacity for biological self-purification, ecological floating beds have been widely utilized in the remediation of eutrophic water bodies. However, most existing studies have focused on the nutrient removal capacities of floating bed plants, with limited attention to their effects on algal community dynamics. In this study, three common aquatic plants—<em>Eichhornia crassipes</em>, <em>Pistia stratiotes</em>, and <em>Ipomoea aquatica</em>—were selected to investigate their overall effects on eutrophic pond remediation. All three species exhibited good tolerance to cyanobacterial blooms and significantly enhanced the anti-oxidative stress response of <em>Cyprinus carpio</em>. The cyanobacterial inhibition rates were 87.92 %, 70.07 %, and 96.32 %, respectively, whereas the relative abundance of green algae increased, most notably with <em>I. aquatica</em>. It is speculated that, in addition to plant-derived allelochemicals, toxin-degrading bacteria from the phyla Proteobacteria, Bacteroidetes, Myxococcota, and Bdellovibrionota may have contributed to the suppression of cyanobacteria. These findings improve understanding of the ecological roles of different floating bed plants in eutrophic pond restoration and offer a theoretical basis for species selection and future application of floating bed systems in aquatic ecosystem management.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"609 ","pages":"Article 742844"},"PeriodicalIF":3.9,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}