Aquaculture最新文献

{"title":"The application of different bottom substrates in Penaeus japonicus culture: Impact on growth, environmental factors, the microbial community and the hepatopancreas transcriptome","authors":"Yulong Du ,&nbsp;Aimin Song ,&nbsp;Luxin Chu,&nbsp;Hongwei Shan","doi":"10.1016/j.aquaculture.2024.742067","DOIUrl":"10.1016/j.aquaculture.2024.742067","url":null,"abstract":"<div><div><em>Penaeus japonicus</em> habitually hides in bottom substrates; hence, it is highly important to choose an appropriate bottom substrate when farming these shrimp. The purpose of this study was to investigate the effects of sand (control), sand mixed with volcanic rock (VR) and sand mixed with oyster shell powder (OS) as bottom substrates in <em>P. japonicus</em> farming. The growth performance, intestinal bacteria, and hepatopancreas transcriptomic information of the shrimp were analyzed, and the physicochemical indicators and the microbial community of the water and bottom substrate were measured. The results showed that the survival rate, specific growth rate, relative weight gain rate and biomass of the shrimp in the OS (75.00 %, 0.83 %/d, 26.4 %, and 0.49 kg/m², respectively) and VR groups (67.50 %, 0.73 %/d, 22.98 %, and 0.43 kg/m², respectively) were higher than those in the control group (62.50 %, 0.35 %/d, 10.73 %, and 0.35 kg/m², respectively), but the differences were not significant (<em>P</em> &gt; 0.05). The concentrations of NO₂⁻-N in the water of the OS (0.22–1.09 mg/L) and VR (0.64–1.17 mg/L) groups were significantly lower than those in the control group (1.92–3.48 mg/L) at 21–28 days (<em>P</em> &lt; 0.05), which reduced the NO₂⁻-N concentration by 68.7 % and 66.3 % at 28 d, respectively. The pH of the bottom substrate in the OS group (8.4–8.7) was significantly greater than that in the control (7.1–8.4) and VR (7.3–8.3) groups (<em>P</em> &lt; 0.05). The indices of α diversity were significantly different between the groups in the water and bottom substrates (<em>P</em> &lt; 0.05). β diversity analysis of the water and bottom substrates revealed significant differences among the three groups (<em>P</em> &lt; 0.05), but there was no significant difference in intestinal bacteria (<em>P</em> &gt; 0.05). Analysis of the differences in bacterial composition revealed significant differences in the abundances of microbial communities in the water and bottom substrates of the VR and OS groups, respectively<em>.</em> Transcriptome analysis revealed that the arachidonic acid pathway was significantly upregulated in the OS group, and the linoleic acid pathway and synaptic vesicle cycle pathway were significantly upregulated in the VR group. Our data indicated that volcanic rock and oyster shell powder could be used as bottom substrates in <em>P. japonicus</em> culture. These results provide a theoretical basis for the selection of bottom substrates for <em>P. japonicus</em> farming.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742067"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple effects of zinc materials on fish, parasites, and the bacterial community in aquatic ecosystems","authors":"Xianliang Li ,&nbsp;Danyi Wei ,&nbsp;Ling Wang ,&nbsp;Hao Wang ,&nbsp;Jundi Shuli ,&nbsp;Wei Hu ,&nbsp;Hongyi Chen ,&nbsp;Shiqian Fang ,&nbsp;Weiliang Guo ,&nbsp;Hengwei Deng ,&nbsp;Dongdong Zhang ,&nbsp;Jianlong Li ,&nbsp;Zhihong Zhong ,&nbsp;Yongcan Zhou","doi":"10.1016/j.aquaculture.2024.742062","DOIUrl":"10.1016/j.aquaculture.2024.742062","url":null,"abstract":"<div><div>Zinc materials show good efficacy in controlling parasitic disease in aquaculture, but their toxicity to other organisms in the aquatic ecosystems remains unknown. In this study, we evaluated the multiple effects of using galvanized materials on fish, parasites, and the bacterial community in aquatic ecosystems. Zinc ion content increased remarkably in seawater after using galvanized mesh (0.4 m²/m³). High zinc concentrations did not cause mortality to the cultured hybrid grouper <em>Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀</em>. Histopathological changes were also not observed in liver and kidney, but serum malondialdehyde (MDA) content increased significantly. Seawater with immersed galvanized mesh (IGM) demonstrated harmful effects on fish parasites <em>Amyloodinium ocellatum</em> dinospores, but little effect on the tomont development. Furthermore, bacterial community diversity increased in cultured seawater after exposure to galvanized mesh for 14 d, with an increase in the α-diversity index. Particularly, pathogenic bacterium <em>Photobacterium damsela</em> and <em>Vibrio</em> decreased significantly in IGM seawater. These results indicate that using zinc materials in aquatic ecosystems can control parasitic disease outbreak and change the bacterial community structure, with little effect on farmed fish.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742062"},"PeriodicalIF":3.9,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of three polychaete species on growth and reproductive performance, biochemical indices, and histological condition of differing tissues in male broodstock of Pacific white shrimp, Litopenaeus vannamei","authors":"Shuaipeng Li ,&nbsp;Hao Liu ,&nbsp;Weibin Huang ,&nbsp;Shipei Yang ,&nbsp;Mingsheng Xie ,&nbsp;Menglong Zhou ,&nbsp;Baiquan Lu ,&nbsp;Biao Li ,&nbsp;Beiping Tan ,&nbsp;Yuanzhi Yang ,&nbsp;Xiaohui Dong","doi":"10.1016/j.aquaculture.2024.742055","DOIUrl":"10.1016/j.aquaculture.2024.742055","url":null,"abstract":"<div><div>A 42-day feeding trial was conducted to evaluate the potential of three polychaetes species to serve as fresh-live maturation diets in terms of growth and reproductive performance, biochemical indices and histological condition of specific tissues in Pacific white shrimp (<em>Litopenaeus vannamei</em>) male broodstock. Two novel polychaete species, <em>Marphysa maxidenticulata</em> and <em>Perinereis nuntia</em>, and a traditional species, <em>Perinereis aibuhitensis</em>, were used as individual diets. A total of 225 healthy male broodstock shrimp were randomly divided into three groups (initial weight 50.40 ± 0.23 g). The nutritional composition of the three polychaete species showed that <em>M. maxidenticulata</em> had the highest crude protein and polyunsaturated fatty acid (FA) content. The results showed that broodstock fed <em>M. maxidenticulata</em> had the highest weight gain and specific growth rates, hepatosomatic (HIS) and gonadosomatic indices (GSI), and molting, with lowest melanization rates. In the <em>M. maxidenticulata</em> group, hepatic and lipoprotein lipases, lipase, trypsin, superoxide dismutase, catalase, glutathione peroxidase, and lysozyme activity were the highest, whereas the malondialdehyde concentration was the lowest. Additionally, triglyceride and testosterone contents were significantly higher in broodstock fed <em>M. maxidenticulata</em> than in the other treatments, particularly in the serum and testes. Furthermore, the histological analysis of the hepatopancreas and gonads revealed that broodstock fed <em>M. maxidenticulata</em> had the largest and most developed B and R cells in the hepatic tubules, the greatest number of seminiferous tubules, the highest sperm density in the testicular lobules, and the largest areas of sperm mass and primary spermatophore layers in the medial vas deferens. The experimental results showed that <em>M. maxidenticulata</em> is a potential new fresh-live diet for male L. <em>vannamei</em> broodstock, and that higher levels of protein and polyunsaturated FAs in <em>M. maxidenticulata</em> can maximize growth, reproductive performance, digestive ability, and antioxidant and immune abilities.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742055"},"PeriodicalIF":3.9,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Alanine enhances crucian carp survival upon Aeromonas hydrophila infection","authors":"Yunchao Cao ,&nbsp;Yuqing Zhou ,&nbsp;Yingli Liu ,&nbsp;Hetron Mweemba Munang'andu ,&nbsp;Bo Peng","doi":"10.1016/j.aquaculture.2024.742061","DOIUrl":"10.1016/j.aquaculture.2024.742061","url":null,"abstract":"<div><div><em>Aeromonas hydrophila</em> is a significant bacterial pathogen that poses a major threat to the sustainable development of aquaculture worldwide. However, effective management of <em>A. hydrophila</em> infection through antibiotic-independent approaches remains insufficiently explored. We have previously shown that oral administration of fructose enhances crucian carp's defense against <em>A. hydrophila</em> infection, but the underlying mechanism is unknown. In this study, we show that fructose reprograms the liver metabolome of crucian carp, resulting in a metabolic shift characterized by increased β-alanine metabolism. Multivariate analysis further identified β-alanine as a key biomarker in this metabolic reprogramming. Subsequent functional studies based on pathway enrichment and multivariate analyses focused on the role of β-alanine. Bacterial challenge assays revealed that β-alanine supplementation, administered either via intraperitoneal injection or dietary feeding, significantly increases the survival rate of crucian carp during <em>A. hydrophila</em> infection. Additionally, β-alanine plays a crucial role in maintaining redox balance, enhancing immunoglobulin production, and promoting immune responses during infection, ultimately reducing bacterial load and improving survival outcomes. This study proposes a metabolism-based approach to manage bacterial infections in aquaculture by modulating the immune response, offering an alternative strategy to traditional antibiotic treatments for bacterial infections.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742061"},"PeriodicalIF":3.9,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultured shrimp Penaeus vanmamei from eastern China accumulates PAHs and PYRs through different pathways: Water and food sources matter","authors":"Juan-ying Li ,&nbsp;Yuxiao Tong ,&nbsp;Qi Gong ,&nbsp;Yaxiong Tian ,&nbsp;Yanhao Yang ,&nbsp;Luting Wen ,&nbsp;Hongjie Chen ,&nbsp;Qian Wang","doi":"10.1016/j.aquaculture.2024.742060","DOIUrl":"10.1016/j.aquaculture.2024.742060","url":null,"abstract":"<div><div>Eastern China is an important area for aquaculture, but the aquaculture environment, as well as the aquatic product quality, are threatened by environmental pollutants such as polycyclic aromatic hydrocarbon (PAHs) and pyrethroid pesticides (PYRs). A three-month monitoring program for PAHs and PYRs throughout the whole culture processes of the shrimp <em>Penaeus vanmamei</em> was carried out. Specifically, concentrations of PAHs and PYRs ranged from 339 ± 19.5–631 ± 75.6 ng·L⁻¹ and 65.3 ± 6.74–93.5 ± 10.8 ng·L⁻¹ in water, 183 ± 33.8–369 ± 25.1 ng·g⁻¹ dw and 441 ± 132–521 ± 62.8 ng·g⁻¹ dw in sediments, while 416 ± 84.3–646 ± 19.7 and 483 ± 23.3–513 ± 22.1 ng·g⁻¹ dw in suspended solids. Mean concentrations of PAHs and PYRs in the adult shrimp were 634 ± 82.3 ng·g⁻¹ ww and 665 ± 58.1 ng·g⁻¹ ww, respectively. A health threat would arise if men consume more than 28.8 g and women consume more than 24.4 g each day. PAHs in the aquaculture environment were generally from the influent water and rainfall, while PYRs were mainly introduced by feed. After entering the culture environment, PAHs primarily increased the water load, leading to more significant bioaccumulation of the shrimp from the water phase (72–95 %). For PYRs, the main sources were feed (44.4 %) and influent water (20.6 %), and they were accumulated in shrimp through feed assimilation directly (15.0 %–32.0 %). Additionally, feed waste containing PYRs increased the contamination burden of sediments and suspended solids, leading to bioaccumulation through assimilation (4.00 %–15.0 %) as well. Although the contribution of influent water to PYRs in the culture environment was lower than that of feed, the contribution of aqueous exposure to the enrichment of PYRs in the shrimp was higher (53.0 %–81.0 %) than that of food (feed, sediments, and suspended solid) intake (19.0 %–47.0 %). The hydrophobicity of PYRs led to an easier bioaccumulation from freely dissolved phase to biota. Therefore, it is important to pay attention to the water quality during the whole culture period, as well as feed quality, to ultimately improve the quality of aquaculture products. In summary, different pollutants in the aquaculture environment and products were from different sources and exposure pathways. Water quality monitoring and improvement are more efficient in decreasing PAHs in the shrimp, while PYRs reduction should be assisted with feed quality control.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742060"},"PeriodicalIF":3.9,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary isoleucine promoted myofiber hypertrophy associated with myonucleus input and protein deposition in fish","authors":"Kaixuan Ren ,&nbsp;Lin Feng ,&nbsp;Pei Wu ,&nbsp;Yang Liu ,&nbsp;Hongmei Ren ,&nbsp;Xiaowan Jin ,&nbsp;Jun Jiang ,&nbsp;Xiaoqiu Zhou ,&nbsp;Weidan Jiang","doi":"10.1016/j.aquaculture.2024.742050","DOIUrl":"10.1016/j.aquaculture.2024.742050","url":null,"abstract":"<div><div>Isoleucine (Ile) is an essential amino-acid, which also has important regulatory functions in animals. Thus, this study was for the first time to investigate effects of dietary Ile on the flesh quality and myofiber hypertrophy in fish. Four hundred and fifty healthy fish (initial weight 615.69 ± 0.03 g) were allotted in 6 experimental groups with 3 replicates each. The basal diet was added different concentrations to comprise six levels of 0.00, 3.00, 6.00, 9.00, 12.00 and 15.00 g/kg of Ile diet, and fed the fish with the experimental diet for 9 weeks. Our data showed that (1)10.81 g Ile/kg diet enhanced (<em>P</em> &lt; 0.05) the Asp, Pro, Ile, Leu, Tyr, Lys, His and IMP contents of fish muscle; (2) Ile increased (<em>P</em> &lt; 0.05) the mRNA and protein levels of MyoD, MyoG, Myf5 and MyHC; (3) Ile enhanced (<em>P</em> &lt; 0.05) the proteins of fibronectin (FN), Integrin-α5 (IN-α5), focal adhesion kinase (FAK), non-receptor tyrosine kinase (Src), cell division cycle 42 (Cdc42), p-target of rapamycin (p-TOR) and p70 ribosomal protein S6 kinase 1 (p-S6K1); (4) Ile decreased (<em>P</em> &lt; 0.05) the protein levels of general control nonderepressible 2 (GCN2), F-box only protein 22 (FBXO22), muscle atrophy F-box (MAFBX) and Muscle RING finger 1 (MURF1). The results conclude that dietary Ile (about 10.81 g/kg) enhanced muscle flavor (mainly including sweet, umami and savory tasting) and changed the fatty acid profile. In addition, we found for the first time that dietary Ile improved muscle myofiber hypertrophy in adult grass carp muscle by promoting myonucleus proliferation and protein deposition.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742050"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel nutritional strategy: Dietary valine improves the health value and nutrient deposition in grass carp (Ctenopharyngodon idella)","authors":"Meng-Ling Cai ,&nbsp;Xiao-Qiu Zhou ,&nbsp;Wei-Dan Jiang ,&nbsp;Pei Wu ,&nbsp;Yang Liu ,&nbsp;Shu-Wei Li ,&nbsp;Ling Tang ,&nbsp;Cheng-Bo Zhong ,&nbsp;Lin Feng","doi":"10.1016/j.aquaculture.2024.742053","DOIUrl":"10.1016/j.aquaculture.2024.742053","url":null,"abstract":"<div><div>Grass carp (<em>Ctenopharyngodon idella</em>) is one of the most economically valuable freshwater fish in China, which is popular among consumers since its high-quality protein and low price. Valine is an essential amino acid for fish. In this study, dietary valine was assessed for the growth performance, muscle nutrient deposition, health value, and sensory quality of sub-adult grass carp. Over 10 weeks, 450 grass carp (539.8 ± 0.89 g) were fed six diets with varying levels of valine (2.8, 6.0, 9.0, 12.4, 16.2 and 19.4 g/kg diet). Results showed that appropriate dietary valine level (9.0 g/kg diet) increased percent weight gain (PWG), feed efficiency (FE) and specific growth rate (SGR), promoting the growth of grass carp. Appropriate dietary valine level improved shear force, pH_0h, and decreased cooking loss and lactate content, improving the sensory quality in the muscle of grass carp. Appropriate dietary valine level improved the muscle contents of crude protein and protein-bound amino acids, might be associated with the up-regulating of IGF-1/PI3K/AKT/TOR/S6K1/4EBP1 signaling pathway and down-regulating the ubiquitin lysosomal pathway. Appropriate dietary valine level increased the muscle contents of crude lipid and unsaturated fatty acids (UFAs), which partly be related to the reason that valine: (1) up-regulating the sterol regulatory element binding protein 1 (SREBP1) signaling pathway to enhance fatty acid synthesis; (2) down-regulating the peroxisome proliferator-activated receptor alpha (PPARα) pathway to inhibit fatty acid decomposition. In summary, dietary valine enhanced the growth performance, muscle nutrients deposition, health value and sensory quality in sub-adult grass carp, which have important guiding significance for the regulation of flesh quality and nutritional studies in fish. According to the broken-line regression analysis, the dietary valine requirement of sub-adult grass carp was 8.96 g/kg diet (34.30 g/kg protein) based on PWG.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742053"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the effects of dietary supplementation with Ampelopsis grossedentata extract and dihydromyricetin on growth performance, muscle quality, and gut microbiota of Megalobrama hoffmanni","authors":"Yunfan Wang ,&nbsp;Yongzhan Mai ,&nbsp;Haiyan Li ,&nbsp;Lingyan Zheng ,&nbsp;Xuesong Wang","doi":"10.1016/j.aquaculture.2024.742049","DOIUrl":"10.1016/j.aquaculture.2024.742049","url":null,"abstract":"<div><div>To investigate whether dihydromyricetin (DMY) mediates the growth-promoting effects of <em>Ampelopsis grossedentata</em> (Ag), a comparative analysis of the effects of DMY and Ag extract (AgE) on <em>Megalobrama hoffmanni</em> was performed. Weight gain rate, specific growth rate, and condition factor of <em>M. hoffmanni</em> in the 0.4 ‰ DMY-supplemented group were significantly lower than those in the 1 ‰ AgE-supplemented group (<em>P</em> &lt; 0.05); however, these indexes in both of the DMY- and AgE-supplemented groups displayed no significant difference with those in the blank control (<em>P</em> &gt; 0.05). Interestingly, hepatosomatic index downregulation and viscerosomatic index upregulation were found after supplemental feeding with DMY or AgE, implying DMY and AgE facilitated hepatic lipid transfer from the liver to mesentery. The total level of muscle fatty acids decreased in the DMY- and AgE-supplemented groups compared to the blank control, and C14:0, C15:0, and C16:0 were significantly downregulated in the 0.4 ‰ DMY-supplemented group (<em>P</em> &lt; 0.05). Lipometabolic <em>Propionibacteriaceae</em> was highly enriched in the DMY- and AgE-supplemented groups, with decreased C14:0 and C16:1n7 via C5-branched dibasic acid metabolism. Additionally, DMY- and AgE-induced lipid peroxidation occurred in the muscle of <em>M. hoffmanni</em>, with increased levels of malondialdehyde, aldehydes (e.g., butanal and hexanal), esters, (e.g., ethyl hexanoate), and ketones (e.g., 2-butanone and 2-pentanone), which contributed to decreased muscle fatty acids and a change in muscle flavor. Because the DMY content of 1 ‰ AgE was equivalent to that of 0.4 ‰ DMY, we confirmed that dietary supplementation with DMY at a relatively high level was responsible for the lipid-lowering effects of Ag, rather than the growth-promoting effects of Ag.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742049"},"PeriodicalIF":3.9,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of nucleic acid amplification based white spot syndrome virus (WSSV) detection methods in Penaeus vannamei","authors":"Jinyu Yang ,&nbsp;Lu Zhang ,&nbsp;Lisui Bao ,&nbsp;Mengqiang Wang","doi":"10.1016/j.aquaculture.2024.742052","DOIUrl":"10.1016/j.aquaculture.2024.742052","url":null,"abstract":"<div><div>The white spot syndrome virus (WSSV) posed a serious threat to shrimp farming, which caused enormous economic losses due to its 100 % mortality rate and rapid spread. At present, a lot of molecular diagnostic methods are utilized to diagnose WSSV, including classic polymerase chain reaction (PCR), nested PCR, loop-mediated isothermal amplification (LAMP), quantitative real-time PCR (qPCR), and recombinant polymerase amplification (RPA). In this study, we compared 17 published molecular diagnostic methods and their primers/probes, including sensitivity, specificity, stability, and practicality. The sensitivities of PCR-1, PCR-2, PCR-3, and PCR-4 were 10² copies/μL, 10¹ copies/μL, 10³ copies/μL, and 10⁴ copies/μL, respectively. The sensitivity of nested PCR was 10⁰ copies/μL. The sensitivities of qPCR-1, qPCR-2, qPCR-3, qPCR-4, qPCR-5, and qPCR-6 had sensitivities of 10² copies/μL, 10⁰ copies/μL, 10⁰ copies/μL, 10⁰ copies/μL, 10² copies/μL, and 10² copies/μL, respectively. Among the isothermal amplification methods, LAMP-1, LAMP-2 and LAMP-3 were 10² copies/μL, 10¹ copies/μL and 10⁰ copies/μL, respectively. While RPA-1, RPA-2, and RPA-3 were 10³ copies/μL, 10¹ copies/μL, and 10⁰ copies/μL, respectively. All methods can specifically detect the target gene. Based on the stability assessment analysis, each detection method was essentially unaffected by low concentration background DNA, whereas the four PCR methods, nested PCR, qPCR-1, qPCR-5, qPCR-6, RPA-1, RPA-2, LAMP-2, and LAMP-3, were not affected by high background DNA concentrations. The detection accuracy was 62.5 % for PCR-3, PCR-4, qPCR-1, qPCR-6 and LAMP-1. The PCR-1 and PCR-2 had an accurate rate of 75 %. The detection rates of qPCR-3, qPCR-4, RPA-1, RPA-2 and RPA-3 were 87.5 %. The detection rates of nested-PCR, qPCR-2, qPCR-5, LAMP-2 and LAMP-3 were 100 %. In all the compared methods, the RPA-3 has a sensitivity of 10⁰ copies/μL, high specificity, and the actual detection rate of 87.5 %. RPA also requires no additional technicians or costly response equipment, and it offers the fastest reaction time. Therefore, RPA, as an emerging assay, seems to be more suitable for low-cost field pathogen detection.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742052"},"PeriodicalIF":3.9,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of arginine and calcium on astaxanthin and lipid accumulation in Haematococcus pluvialis under high light conditions","authors":"Adolf Acheampong ,&nbsp;Monia Ravelonandrasana Fetisoa ,&nbsp;Precious Bondzie-Quaye ,&nbsp;Qing Huang","doi":"10.1016/j.aquaculture.2024.742039","DOIUrl":"10.1016/j.aquaculture.2024.742039","url":null,"abstract":"<div><div><em>Haematococcus pluvialis</em> (<em>H. pluvialis</em>) is one of the richest natural sources of astaxanthin (AST). However, since the production of AST by <em>H. pluvialis</em> is unable to meet market demand, it is desirable to increase its productivity. This study provides an innovative strategy to improve astaxanthin and lipids productivity of <em>H. pluvialis</em> by combining arginine (Arg) and calcium (Ca²⁺) under 120 μmol m⁻² s⁻¹ light conditions. Application of Arg promoted astaxanthin and lipid accumulation by 25% and 24.5%, respectively. The co-supplementation of Arg and Ca²⁺ (0.25 mM Arg, 50 μM CaCl₂) further enhanced the astaxanthin and lipid productivity by 40.7% and 32.6% compared to the control. Arg and Ca²⁺ synergically activated the arginine metabolic pathway by triggering ornithine decarboxylase (<em>odc</em>), ornithine aminotransferase (<em>oat</em>), nitric oxide synthase (<em>nos</em>) and argininosuccinate lyase (<em>asl</em>) genes at both stages of cultivation, signifying their importance in growth and stress responses. More importantly, Arg and Ca²⁺ synergically controlled signaling molecules through the calcium channels and promoted astaxanthin and lipids by regulating carotenogenesis and lipogenesis. The results therefore provide a reference for research and application of Arg and Ca²⁺ in microalgae aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"598 ","pages":"Article 742039"},"PeriodicalIF":3.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}