Exosomal miR-4171-x contributes to T helper II cell immune response by regulating the Gata-3 expression in flounder (Paralichthys olivaceus)

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-07-05 DOI:10.1016/j.aquaculture.2025.742930

Xueyan Shi , Lixia Jiang , Heng Chi , Roy Ambli Dalmo , Xiaoqian Tang , Jing Xing , Xiuzhen Sheng , Wenbin Zhan

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引用次数: 0

Abstract

Exosomes are recognized for their function as facilitators of intercellular communication and regulators of immune responses. However, the specific contributions of exosomes to the host's immune response against pathogens, especially concerning T cell immunity, remain inadequately elucidated in teleost fish. This study characterized exosomal miRNAs from the peritoneal cavity of flounder (Paralichthys olivaceus) challenged with Vibrio anguillarum. Exosomes isolated from uninfected and V. anguillarum-challenged flounder exhibited typical cup-shaped morphology and size distribution, and were further confirmed by the expression of exosomal markers (Cd9, Cd81, and Tsg101). miRNA transcriptome sequencing revealed that the expression profiles of 69 exosomal miRNAs exhibited significant alterations when comparing the infected group to the control group. Specifically, 49 miRNAs were found to be significantly upregulated, while 20 miRNAs were significant downregulation. Furthermore, GO and KEGG enrichment analyses indicated that the predicted target genes of these significantly differentially expressed miRNAs were involved in various immune processes, including signaling pathways related to T cell immune response. Functional studies identified exosomal miR-4171-x as a critical regulator, which mechanistically targets gata3—the master transcriptional regulator of Th2 cell differentiation. These findings provided novel insights into host-pathogen interactions and may inform future strategies for improving disease resistance in aquaculture.

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外泌体miR-4171-x通过调节牙鲆Gata-3表达参与辅助性T细胞免疫应答

外泌体被认为具有促进细胞间通讯和调节免疫反应的功能。然而，外泌体对宿主对抗病原体的免疫反应，特别是T细胞免疫的具体贡献，在硬骨鱼中仍未充分阐明。本研究对鳗弧菌攻毒后的比目鱼腹膜腔外泌体mirna进行了研究。从未感染和鳗源弧菌侵染的牙鲆中分离的外泌体具有典型的杯状形态和大小分布，并通过外泌体标志物（Cd9、Cd81和Tsg101）的表达进一步证实了这一点。miRNA转录组测序显示，感染组与对照组相比，69个外泌体miRNA的表达谱发生了显著变化。其中49个mirna显著上调，20个mirna显著下调。此外，GO和KEGG富集分析表明，这些显著差异表达的mirna的预测靶基因参与各种免疫过程，包括与T细胞免疫应答相关的信号通路。功能研究发现外泌体miR-4171-x是一个关键的调节因子，其机制是靶向gata3 - Th2细胞分化的主要转录调节因子。这些发现为宿主-病原体相互作用提供了新的见解，并可能为未来提高水产养殖抗病能力的策略提供信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.