Orange-shell phenotype regulated by CgABCG2-mediated protoporphyrin IX transport in Pacific oyster (Crassostrea gigas)

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-17 DOI:10.1016/j.aquaculture.2025.743212

Biyang Hu , Hong Yu , Lingfeng Kong , Shikai Liu , Qi Li

{"title":"Orange-shell phenotype regulated by CgABCG2-mediated protoporphyrin IX transport in Pacific oyster (Crassostrea gigas)","authors":"Biyang Hu , Hong Yu , Lingfeng Kong , Shikai Liu , Qi Li","doi":"10.1016/j.aquaculture.2025.743212","DOIUrl":null,"url":null,"abstract":"<div><div>Animal body coloration, governed by pigment synthesis and transport dynamics, carries ecological and commercial significance in aquaculture species. In molluscs, appealing shell color directly enhances market value of commercially important species and reflects physiological status, yet molecular mechanisms underlying pigment transportation remain unresolved. While porphyrins serve as primary pigments driving coloration, their transport pathways and deposition processes are poorly characterized. The Pacific oyster (<em>Crassostrea gigas</em>), with heritable shell color diversity, provides an ideal model for deciphering pigment transport mechanisms and improving color-trait-based breeding. Here, we identify ATP binding cassette subfamily G member 2 (<em>Cg</em>ABCG2) as the transporter mediating cytoplasmic Protoporphyrin IX (PPIX) efflux. Multi-omics analyses revealed stage specific synchronization between PPIX accumulation and <em>Cg</em>ABCG2 upregulation during shell formation. Western blotting, quantitative real-time PCR, and immunofluorescence demonstrated positive correlations between <em>Cg</em>ABCG2 expression levels and orange hue intensity, while structural modeling confirmed high PPIX binding affinity. Furthermore, <em>Cg</em>ABCG2 knockdown experiments impact porphyrin metabolism and PPIX accumulation, compromising orange-shell coloration. This study provides the first experimental evidence of ABCG2 mediated PPIX transmembrane transport in mollusc, extends the established regulatory network of shell coloration and offers potential molecular targets for genetically enhancing color traits in aquaculture species</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"612 ","pages":"Article 743212"},"PeriodicalIF":3.9000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044848625010981","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Animal body coloration, governed by pigment synthesis and transport dynamics, carries ecological and commercial significance in aquaculture species. In molluscs, appealing shell color directly enhances market value of commercially important species and reflects physiological status, yet molecular mechanisms underlying pigment transportation remain unresolved. While porphyrins serve as primary pigments driving coloration, their transport pathways and deposition processes are poorly characterized. The Pacific oyster (Crassostrea gigas), with heritable shell color diversity, provides an ideal model for deciphering pigment transport mechanisms and improving color-trait-based breeding. Here, we identify ATP binding cassette subfamily G member 2 (CgABCG2) as the transporter mediating cytoplasmic Protoporphyrin IX (PPIX) efflux. Multi-omics analyses revealed stage specific synchronization between PPIX accumulation and CgABCG2 upregulation during shell formation. Western blotting, quantitative real-time PCR, and immunofluorescence demonstrated positive correlations between CgABCG2 expression levels and orange hue intensity, while structural modeling confirmed high PPIX binding affinity. Furthermore, CgABCG2 knockdown experiments impact porphyrin metabolism and PPIX accumulation, compromising orange-shell coloration. This study provides the first experimental evidence of ABCG2 mediated PPIX transmembrane transport in mollusc, extends the established regulatory network of shell coloration and offers potential molecular targets for genetically enhancing color traits in aquaculture species

查看原文本刊更多论文

cgabcg2介导的原卟啉IX转运对太平洋牡蛎橙壳表型的调控

动物体色受色素合成和运输动力学的支配，在水产养殖物种中具有生态和商业意义。在软体动物中，吸引人的贝壳颜色直接提高了重要商业物种的市场价值，反映了其生理状态，但色素转运的分子机制尚不清楚。虽然卟啉作为主要色素驱动着色，但它们的运输途径和沉积过程的特征很差。太平洋牡蛎（Crassostrea gigas）具有可遗传的外壳颜色多样性，为破解色素转运机制和改进基于颜色性状的育种提供了理想的模型。在这里，我们确定ATP结合盒亚家族G成员2 （CgABCG2）是介导细胞质原卟啉IX （PPIX）外排的转运体。多组学分析显示，在壳形成过程中，PPIX积累和CgABCG2上调之间存在阶段性同步。Western blotting、实时荧光定量PCR和免疫荧光均显示CgABCG2表达水平与橙色色调强度呈正相关，而结构模型证实PPIX具有较高的结合亲和力。此外，CgABCG2敲低实验影响卟啉代谢和PPIX积累，影响橙壳的颜色。本研究为ABCG2介导的PPIX在贝类中的跨膜转运提供了第一个实验证据，扩展了已建立的贝壳着色调控网络，并为水产养殖物种遗传增强颜色性状提供了潜在的分子靶点

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.