A broad-spectrum vibriophage with potential for phage therapy against Vibrio alginolyticus in shrimp aquaculture

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-18 DOI:10.1016/j.aquaculture.2025.743215

Keming Shi , Huiyu Ding , Wanxuan Zhong , Wenqing Li , Min Hsiao , Bu Xu , Haipeng Guo , Jing Zhao , Jie Xu , Demin Zhang , Yunlan Yang , Rui Zhang

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Abstract

Phage therapy, a promising alternative to antibiotics, has gained traction in aquaculture for combating bacterial diseases. This study investigated the potential application of a lytic phage, vB_ValS_R12Z (R12Z), against Vibrio alginolyticus ATCC 17749^T, in aquaculture by characterizing its biological and genetic properties. Phage R12Z displayed a siphovirus morphotype, characterized by a short latent period of 35 min and a large burst size of 105 ± 27 plaque forming units (PFU) per infected cell. Intergenomic similarity analysis revealed that R12Z belongs to the same species within the genus Mardecavirus as phages KF6 and HCMJ, with no antibiotic resistance factor-related genes or virulence factors detected. Host range experiments demonstrated that R12Z is capable of infecting at least three Vibrio species, including seven strains of V. alginolyticus, one strain of V. parahaemolyticus, and one strain of V. natriegens. The strong lytic capacity and high bacteriostatic capability of R12Z were observed with an average bacteriolytic efficiency exceeding 75 % across all multiplicities of infection (0.001–10). In addition, R12Z showed robust environmental stability, enduring pH levels between 4 and 11, and temperatures spanning 4 °C–50 °C. R12Z showed effective protection of shrimp postlarvae from V. alginolyticus, resulting in higher survival rates. Phage R12Z is therefore a potential therapeutic agent against vibriosis in aquaculture, offering promising prospects for disease management in aquatic settings.

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一种广谱噬菌体，可用于对虾养殖中抗溶藻弧菌的噬菌体治疗

噬菌体疗法是一种很有前途的抗生素替代品，在水产养殖中用于对抗细菌性疾病已经获得了吸引力。本研究通过对一种抗溶藻弧菌ATCC 17749T的裂解噬菌体vB_ValS_R12Z （R12Z）的生物学和遗传特性的研究，探讨了其在水产养殖中的应用潜力。噬菌体R12Z表现为siphovirus型，潜伏期短（35 min），爆发量大（每感染细胞105±27个斑块形成单位）。基因组间相似性分析显示，R12Z与噬菌体KF6和HCMJ属于马尔地卡病毒属同一种，未检出抗生素耐药因子相关基因或毒力因子。宿主范围实验表明，R12Z能够感染至少3种弧菌，包括7株溶藻弧菌、1株副溶血性弧菌和1株营养弧菌。R12Z具有较强的溶菌能力和较高的抑菌能力，在所有感染倍数中平均溶菌效率均超过75%（0.001-10）。此外，R12Z具有良好的环境稳定性，pH值在4到11之间，温度范围在4°C到50°C之间。R12Z对溶藻弧菌对虾幼虫有较好的保护作用，具有较高的成活率。因此，噬菌体R12Z是一种潜在的水产养殖弧菌病治疗剂，为水产养殖环境中的疾病管理提供了广阔的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.