A rapid method to generate an all-female population in common carp (Cyprinus carpio) using the neomale carp of F0 population with targeted aromatase mutations

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-12 DOI:10.1016/j.aquaculture.2025.743185

Yuting Xie , Hongfei Bao , Guanghui Chen , Xuehui Li , Yonglin Ruan , Qiyong Lou , Xia Jin , Jiangyan He , Tianshu Zhang , Jian Wang , Xueqiao Qian , Gang Zhai , Zhan Yin

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引用次数: 0

Abstract

Teleosts are excellent models for elucidating the regulatory mechanisms of gonadal differentiation. Although the essential role of ovarian aromatase (encoded by cyp19a1a) in ovarian differentiation has been documented in several species of fish, this remains poorly understood in common carp (Cyprinus carpio) due to its long period of sexual maturity and the complexity of the multiple loci in its allotetraploid genome. Here, using the CRISPR/Cas9 strategy, some neomale carp with chimeric mutation (CM) of cyp19a1aA, cyp19a1aB or cyp19a1aA;cyp19a1aB were found in the F0 population at 1 year post-hatching (yph). The mutation ratios of cyp19a1aA, cyp19a1aB, or cyp19a1aA;cyp19a1aB were positively correlated with the ratio of sex reversal from female to male. Concentrations of estradiol were significantly decreased in female and neomale of chimaeras carp compared to wild-type (WT) control female carp. The progeny from artificial fertilization between WT female carp and these neomale carp were confirmed as all-female (AF) carp, as evidenced by genetic identification of male-specific DNA marker and histological analysis of progeny gonads. Finally, in comparison to the control group, which comprised both male and female carp reared in the same pond, AF carp at 4 months post-hatching (mph) demonstrated enhanced 12.06 % growth performance. In conclusion, we have shown that both Cyp19a1aA and Cyp19a1aB are required for estrogen synthesis, which is essential for ovarian differentiation in common carp. Therefore, a rapid method to generate AF populations using the F0 CM populations has been established by genetic manipulation targeting the loci of the cyp19a1a genes. The present study has revealed the non-redundant roles of cyp19a1aA and cyp19a1aB in ovarian differentiation and also provides an efficient and rapid method of controlling gonadal differentiation in fish without knowledge of the specific sex-determining gene (SDG) of the particular fish.

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利用F0种群的新雌鱼靶向芳香酶突变，快速培育鲤鱼全雌种群

硬骨鱼是阐明性腺分化调控机制的极好模型。虽然卵巢芳香化酶（由cyp19a1a编码）在多种鱼类卵巢分化中的重要作用已被证实，但由于鲤鱼（Cyprinus carpio）的性成熟期较长，且其异源四倍体基因组中多个位点的复杂性，因此对其的了解仍然很少。本研究利用CRISPR/Cas9策略，将一些cyp19a1aA、cyp19a1aB或cyp19a1aA嵌合突变（CM）的新雌鲤鱼；在孵化后1年（yph） F0种群中发现cyp19a1aB。cyp19a1aA、cyp19a1aB、cyp19a1aA的突变比例；cyp19a1aB与性别反转比例呈正相关。雌性和新雄性嵌合鲤鱼体内雌二醇浓度与野生型对照相比显著降低。通过雄性特异性DNA标记的遗传鉴定和子代性腺的组织学分析，证实WT雌鱼与这些新雄鱼人工受精后的后代为全雌（AF）鲤鱼。最后，与在同一池塘中饲养雄性和雌性鲤鱼的对照组相比，孵化后4个月（mph）的AF鲤鱼的生长性能提高了12.06%。综上所述，我们发现Cyp19a1aA和Cyp19a1aB都是雌性激素合成所必需的，而雌性激素是鲤鱼卵巢分化所必需的。因此，通过针对cyp19a1a基因位点的遗传操作，建立了一种利用F0 CM群体快速生成AF群体的方法。本研究揭示了cyp19a1aA和cyp19a1aB在卵巢分化中的非冗余作用，也为不知道特定鱼类的特定性别决定基因（SDG）的情况下控制鱼类性腺分化提供了一种高效、快速的方法。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.