Transcription of immune genes in ranched southern bluefin tuna show correlations to Cardicola spp. (Digenea: Aporocotylidae) infection

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-06-24 DOI:10.1016/j.aquaculture.2025.742877

Maree Widdicombe , Sarah Eastwood , Bronwyn E. Campbell , Cecilia Power , Barbara F. Nowak , Paul A. Ramsland , Nathan J. Bott

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引用次数: 0

Abstract

Cardicola forsteri and C. orientalis (Digenea: Aporocotylidae) are significant pathogens affecting the Southern bluefin tuna (SBT), Thunnus maccoyii, aquaculture industry. The aims of this research were to identify a relationship between immune gene expression of SBT and C. forsteri and C. orientalis infection burdens; and to understand how immune gene expression changes over time during commercial ranching. SBT were collected during commercial operations in 2022 after 4, 8 and 10 weeks of ranching and the transcription of interleukin 1 beta (il1b), interleukin 8 (il8), tumour necrosis factor 2 (tnf2), immunoglobulin m (igm), major histocompatibility complex 2 (mhc2) and t cell receptor beta (tcrb) were measured from the anterior kidney and gills using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Cardicola forsteri and C. orientalis infection was quantified as number of eggs/mm of SBT gill filament, number of adult flukes in SBT heart and C. forsteri and C. orientalis ITS-2 rDNA copy number/mg in SBT gills. Expression of il8 and igm in anterior kidney was positively correlated to C. forsteri and C. orientalis eggs/mm of SBT gill filament and to the copy number of C. forsteri ITS-2 rDNA in SBT gills. Expression of igm and mhc2 was higher in SBT positive for adult flukes and the expression of igm, mhc2 and tcrb in the anterior kidney was positively correlated to the number of adult flukes in SBT heart. Expression of tcrb in gills was negatively correlated to the number of adult flukes in SBT heart and negatively correlated to the copy number of C. orientalis ITS-2 rDNA. Expression of the adaptive immune genes igm, mhc2 and tcrb increased over the ranching duration in the kidney of SBT and the innate immune genes il1b and il8 decreased over the ranching duration in SBT gills. Despite evidence of an immune response to C. forsteri and C. orientalis, SBT were not able to clear infections during the standard industry ranching timeline.

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南部养殖蓝鳍金枪鱼免疫基因转录与Cardicola spp. （Digenea: Aporocotylidae）感染相关

Cardicola forsteri和C. orientalis （Digenea: Aporocotylidae）是影响南方蓝鳍金枪鱼（SBT）、鲭鱼（Thunnus maccoyii）水产养殖业的重要病原体。本研究的目的是确定SBT免疫基因表达与福氏弧菌和东方弧菌感染负荷之间的关系；并了解在商业牧场期间免疫基因表达如何随时间变化。饲养4、8和10周后，于2022年商业手术中收集SBT，采用逆转录定量聚合酶链式反应（RT-qPCR）检测前肾和鳃中白细胞介素1 β (il1b)、白细胞介素8 （il8）、肿瘤坏死因子2 （tnf2）、免疫球蛋白m （igm）、主要组织相容性复合体2 （mhc2）和t细胞受体β (tcrb)的转录。以SBT鳃丝虫卵数/mm、SBT心脏成虫吸虫数、SBT鳃中forsteri和东方弓形虫ITS-2 rDNA拷贝数/mg为感染指标。前肾il8和igm的表达与SBT鳃丝中forsteri和orientalis卵数/mm及SBT鳃中forsteri ITS-2 rDNA拷贝数呈正相关。igm和mhc2的表达在SBT阳性的成年吸虫中较高，igm、mhc2和tcrb在前肾中的表达与SBT心脏中成年吸虫的数量呈正相关。鳃中tcrb的表达与SBT心脏吸虫成虫数呈负相关，与东方鱼ITS-2 rDNA拷贝数呈负相关。适应性免疫基因igm、mhc2和tcrb在SBT肾脏的表达随放牧时间的延长而增加，先天免疫基因il1b和il8在SBT鳃中的表达随放牧时间的延长而降低。尽管有证据表明对福氏弧菌和东方弧菌有免疫反应，但在标准的工业牧场时间内，SBT无法清除感染。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.