IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-10 DOI: 10.1080/01652176.2025.2485480

Siyuan Jia, Andrea R McWhorter, Samiullah Khan, Daniel M Andrews, Gregory J Underwood, Kapil K Chousalkar

{"title":"In vitro and in vivo study on the effects of nutritive media compared with water on the resuscitation of a live attenuated Salmonella Typhimurium vaccine.","authors":"Siyuan Jia, Andrea R McWhorter, Samiullah Khan, Daniel M Andrews, Gregory J Underwood, Kapil K Chousalkar","doi":"10.1080/01652176.2025.2485480","DOIUrl":"https://doi.org/10.1080/01652176.2025.2485480","url":null,"abstract":"In this study, a commercially available Salmonella Typhimurium vaccine (Vaxsafe ST) reconstituted in either water or nutrient broth, was tested to understand its effects on the in vitro invasion of Caco-2 cells and its transcriptional regulation post-reconstitution. Over time, the invasiveness of Vaxsafe ST was significantly higher following reconstitution in nutrient broth compared with water. Incubation temperature post-reconstitution did not significantly affect the invasion rate. Transcriptome data showed that Vaxsafe ST reconstituted in nutrient broth upregulated genes involved in the two-component system and flagella activity pathways; however, genes involved in host colonization and invasion were unaltered. Genes involved in host colonization and invasion were downregulated after reconstitution in water. Vaxsafe ST reconstituted in a nutritive diluent improved the metabolic activities of the vaccine. The animal experiment demonstrated that vaccine colonization was significantly higher in caeca compared with ileum irrespective of the diluent. Incubating the vaccine in nutrient broth for 30 min before administration did not significantly increase its colonization or organ invasion in chicks. Overall, the findings support the use of nutritive media for the reconstitution of Vaxsafe ST, warranting further investigation to optimize vaccine performance. This study provides a foundation for future work on vaccine reconstitution strategies.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-10"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11986869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits. 肌生成抑制素缺乏家兔骨骼肌发育中circrna和lncrna参与的综合分析

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-26 DOI: 10.1080/10495398.2025.2465624

Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan

{"title":"Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits.","authors":"Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan","doi":"10.1080/10495398.2025.2465624","DOIUrl":"10.1080/10495398.2025.2465624","url":null,"abstract":"Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three WT and four MSTN KO rabbits, we analyzed the expression profiles of circRNAs and lncRNAs. After identifying the differently expressed RNAs, the biological pathways implicated were ascertained by performing enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). We identified differences in the expression of 251 circRNAs (79 upregulated and 172 downregulated), 176 lncRNAs (53 upregulated and 123 downregulated), and 1178 mRNAs (408 upregulated and 770 downregulated) between WT and MSTN KO rabbits. Target genes were significantly enriched in pathways associated with protein synthesis and catabolism, such as oxidative phosphorylation, ubiquitin-mediated proteolysis, the FoxO signaling pathway, and the pentose phosphate pathway, as identified through GO and KEGG enrichment analyses. The constructed network indicates that a class of circRNAs and lncRNAs is engaged in MSTN-mediated regulation of skeletal muscle development. These findings provide valuable insights for innovative therapeutic, diagnostic, and preventive approaches to muscle disorders.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2465624"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle. 重组细胞外包膜病毒粒子候选蛋白检测牛肿块性皮肤病病毒血清学反应的评价。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/01652176.2025.2475989

Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul

{"title":"Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle.","authors":"Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul","doi":"10.1080/01652176.2025.2475989","DOIUrl":"10.1080/01652176.2025.2475989","url":null,"abstract":"Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic insights into Mycobacterium orygis infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle. 转录组学对牛羊分枝杆菌感染相关的肺肉芽肿揭示了多细胞免疫网络和结核病生物标志物。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-05-27 DOI: 10.1080/01652176.2025.2509503

Rishi Kumar, Sripratyusha Gandham, Vinay Bhaskar, Manas Ranjan Praharaj, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey

{"title":"Transcriptomic insights into Mycobacterium orygis infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle.","authors":"Rishi Kumar, Sripratyusha Gandham, Vinay Bhaskar, Manas Ranjan Praharaj, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey","doi":"10.1080/01652176.2025.2509503","DOIUrl":"10.1080/01652176.2025.2509503","url":null,"abstract":"Mycobacterium orygis, a member of the Mycobacterium tuberculosis complex (MTBC), has emerged as a significant contributor to tuberculosis (TB) in cattle, wildlife, and humans. However, understanding about its pathogenesis and severity is limited, compounded by the lack of reliable TB biomarkers in cattle. This study delves into the comparative pathology and transcriptomic landscape of pulmonary granulomas in cattle naturally infected with M. orygis, using high-throughput RNA sequencing. Histopathological analysis revealed extensive, multistage granulomatous, necrotic, and cavitary lesions, indicative of severe lung pathology induced by M. orygis. Transcriptomic profiling highlighted numerous differentially expressed genes and dysregulated pathways related to immune response modulation and extracellular matrix remodelling. Additionally, cell type enrichment analysis provided insights into the multicellularity of the granulomatous niche, emphasising complex cell-cell interactions within TB granulomas. Via comparative transcriptomics leveraging publicly available bovine and human TB omics datasets, 14 key immunomodulators (SOD2, IL1α/β, IL15, IL18, CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CCL8/MCP-2, CCL20/MIP-3α, CXCL2/MIP-2, CXCL10/IP-10, CXCL11, and IFN-γ) were identified as potential biomarkers for active TB in cattle. These findings significantly advance our understanding of M. orygis pathogenesis in bovine TB and highlight potential targets for the development of diagnostic tools for managing and controlling the disease.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-19"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens. 建立鸡白痢沙门氏菌TaqMan qPCR检测方法。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-30 DOI: 10.1080/01652176.2025.2454473

Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue

{"title":"Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens.","authors":"Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue","doi":"10.1080/01652176.2025.2454473","DOIUrl":"10.1080/01652176.2025.2454473","url":null,"abstract":"Salmonella Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Salmonella Pullorum, enabling precise differentiation from other Salmonella serovars. The assay's detection limit was 5 copies/μL of plasmid and 4 CFU/μL of bacterial DNA. Furthermore, we collected 676 chicken samples from an established infection model to compare the performance of the TaqMan qPCR assay with traditional bacterial culturing and antibody-based detection approaches. With superior sensitivity and specificity, the newly developed method detected over 80% of positive chickens, significantly outperforming the two conventional methods. Moreover, we proposed a combined framework that incorporates the advantages of TaqMan qPCR assay and antibody detection method, further enhancing the detection rate of positives to 92%. Additionally, to address the frequent aerosol contamination of amplification products in laboratory settings, we devised an easy-to-deploy anti-contamination system based on T7 exonuclease. Overall, the T7 exonuclease-assisted TaqMan qPCR assay will not only upgrade the current detection for pullorum disease, but also exemplify the feasibility of targeting specific genomic deletions for pathogen detection.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL14 and WTAP play a crucial role in the regulation of bovine preadipocyte differentiation. METTL14和WTAP在牛前脂肪细胞的分化调控中起重要作用。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2476531

Jia Liu, Chicheng Ma, Yu Cheng, Minzhi Wang, Guoqing Zhao, Liwei Huang, Ruigao Song, Xi Wang, Hongxia Li

引用次数: 0

Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review. 了解东南亚野生动物的人畜共患病病原体和危险因素：系统的文献综述。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/01652176.2025.2475990

Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee

{"title":"Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review.","authors":"Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee","doi":"10.1080/01652176.2025.2475990","DOIUrl":"10.1080/01652176.2025.2475990","url":null,"abstract":"The COVID-19 pandemic has demonstrated the significance of the human-animal interface in the emergence of zoonotic diseases, with wildlife serving as an important source of infection. A better understanding of the specific pathogens and mechanisms involved is vital to prepare against future outbreaks, especially in Southeast Asia, a hotspot for zoonotic diseases. This paper reviews the published literature on wildlife zoonoses in this region from 2012 to 2022. The results show a diverse range of potential zoonotic pathogens and the widespread occurrence of zoonotic diseases from wildlife. Drivers of zoonotic pathogen spillover include (i) environmental factors (e.g. animal habitat disruption, environmental conditions, exposure to contaminated water/food/soil), (ii) animal factors (e.g. movement patterns, age-related susceptibility), (iii) human factors (e.g. lack of awareness, poor hygiene practices, age, gender and income) and (iv) human-animal-environmental interface factors (e.g. close contact between humans and animals, exposure through visiting animals and presence of vectors). The diverse drivers of zoonoses in Southeast Asia put its communities at risk for infection. To mitigate these risks, global health efforts should consider adopting a One Health approach to foster collaboration across human, animal, and wildlife health sectors. This could involve educating communities on safe animal interactions and improving disease surveillance.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing. 基于全基因组重测序的莆田黑鸭蛋重性状候选基因鉴定

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-17 DOI: 10.1080/10495398.2025.2503754

Yinhua Yang, Weilong Lin, Huihuang Li, Fan Yang, Xinguo Bao, Chengfu Pan, Lianjie Lai, Weimin Lin, Ruiyi Lin

{"title":"Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing.","authors":"Yinhua Yang, Weilong Lin, Huihuang Li, Fan Yang, Xinguo Bao, Chengfu Pan, Lianjie Lai, Weimin Lin, Ruiyi Lin","doi":"10.1080/10495398.2025.2503754","DOIUrl":"https://doi.org/10.1080/10495398.2025.2503754","url":null,"abstract":"Egg weight is a primary economic trait in poultry breeding. Putian Black duck, an excellent local laying duck breed in Fujian Province, includes two different strains, black feather strain and white feather strain. The white feather strain of Putian Black duck is also known as Putian White duck. Except for the different feather colors, these two strains differ in egg weight. In this study, whole-genome resequencing was conducted on Putian Black duck and Putian White duck to explore the differences in the genetic mechanism of egg weight. LRP8, VLDLR, and LPL were identified as key candidate genes affecting egg weight. Mass spectrometry was used to detect the SNPs of LRP8, VLDLR, and LPL. Result indicates that the SNPs of LRP8, VLDLR, and LPL in both populations exhibited moderate polymorphism, and Putian Black duck possessed higher genetic variation and potential selectivity. Association analysis indicated that in Putian Black duck, four SNPs in the LRP8 gene were significantly associated with egg weight. These loci can be used as molecular markers for improving egg weight in Putian Black duck.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503754"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings. 腰骶椎间盘退变犬的高强度区：来自MRI和组织病理学结果的见解。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-07 DOI: 10.1080/01652176.2025.2486765

S Amir Kamali, Michelle Teunissen, Dirk Hendrik Nicolaas van den Broek, Elisabeth M Burgers, Guy C M Grinwis, Keita Ito, Marianna A Tryfonidou, Björn P Meij

{"title":"High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings.","authors":"S Amir Kamali, Michelle Teunissen, Dirk Hendrik Nicolaas van den Broek, Elisabeth M Burgers, Guy C M Grinwis, Keita Ito, Marianna A Tryfonidou, Björn P Meij","doi":"10.1080/01652176.2025.2486765","DOIUrl":"10.1080/01652176.2025.2486765","url":null,"abstract":"The diagnosis and management of lumbosacral pain in dogs is challenging, requiring thorough examination, with MRI playing a crucial diagnostic role. This retrospective study investigates the presence of high-intensity zones (HIZ) in the dorsal annulus fibrosus (AF) of the lumbosacral region on MRI and describes the corresponding histopathological features in dogs with intervertebral disc (IVD) degeneration. T2-weighted (T2W) and T1-weighted (T1W) sagittal MRI scans were evaluated using a classification system developed in human medicine to analyze HIZ characteristics. Among 836 dogs with IVD degeneration, 57 (6.8%) exhibited T2W HIZ, with a median age of 7 years and median weight of 33.7 kg. All cases with HIZ consistently exhibited radiological degenerative lumbosacral stenosis. The most common T2W HIZ shape was round (43%), while 14% of lesions also appeared hyperintense on T1W. Histopathological analysis of 11 dorsal AF samples collected during standard-of-care decompressive surgery revealed two patterns: reactive cystic structures (3/11) and granulation tissue (8/11), with differential MRI presentation. This is the first study to document HIZ in the lumbosacral level of dogs with IVD degeneration. With this recognition, prospective analyses and their correlation with clinical presentations will be essential in determining the role and prognostic significance of HIZ.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat. 南江黄山羊IGF2BP1第二内含子snp的鉴定及其与生长性状的关系

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/10495398.2025.2461176

Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao

{"title":"Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat.","authors":"Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao","doi":"10.1080/10495398.2025.2461176","DOIUrl":"10.1080/10495398.2025.2461176","url":null,"abstract":"Insulin-like Growth Factor 2 mRNA-binding Protein 1 (IGF2BP1) is a candidate gene of significant interest for modulating economically important traits in livestock and poultry. The second intron of IGF2BP1 has been implicated in growth-related traits, though its precise mechanistic role remains elusive. Initial resequencing analyses in our laboratory indicated strong selective pressures on the IGF2BP1 genomic region, prompting the selection and identification of several single nucleotide polymorphisms (SNPs). Seven SNPs were mapped to the conserved region of the second intron, necessitating further investigation into their functional relevance and association with growth traits. In this study, 348 Nanjiang Yellow goats were analyzed, and the association analysis via the GLM program in SAS 9.4 identified five SNPs significantly correlated with growth traits. Notably, rs652062749(A > G) emerged as a critical locus influencing later-stage growth traits. Furthermore, strong linkage disequilibrium was observed among three SNPs, with the rs638185407 (T > A) variant markedly enhancing luciferase activity in H293T cells. Combination genotypes TTAACT, TTCCCC, and ATCACT were identified as superior for growth traits, offering theoretical insights for genetic co-breeding. This study underscores the potential utility of IGF2BP1 as a functional genetic marker in Nanjiang Yellow goat breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2461176"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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