农林科学最新文献_第2页

Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/01652176.2025.2475990

Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee

{"title":"Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review.","authors":"Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee","doi":"10.1080/01652176.2025.2475990","DOIUrl":"10.1080/01652176.2025.2475990","url":null,"abstract":"The COVID-19 pandemic has demonstrated the significance of the human-animal interface in the emergence of zoonotic diseases, with wildlife serving as an important source of infection. A better understanding of the specific pathogens and mechanisms involved is vital to prepare against future outbreaks, especially in Southeast Asia, a hotspot for zoonotic diseases. This paper reviews the published literature on wildlife zoonoses in this region from 2012 to 2022. The results show a diverse range of potential zoonotic pathogens and the widespread occurrence of zoonotic diseases from wildlife. Drivers of zoonotic pathogen spillover include (i) environmental factors (e.g. animal habitat disruption, environmental conditions, exposure to contaminated water/food/soil), (ii) animal factors (e.g. movement patterns, age-related susceptibility), (iii) human factors (e.g. lack of awareness, poor hygiene practices, age, gender and income) and (iv) human-animal-environmental interface factors (e.g. close contact between humans and animals, exposure through visiting animals and presence of vectors). The diverse drivers of zoonoses in Southeast Asia put its communities at risk for infection. To mitigate these risks, global health efforts should consider adopting a One Health approach to foster collaboration across human, animal, and wildlife health sectors. This could involve educating communities on safe animal interactions and improving disease surveillance.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL14 and WTAP play a crucial role in the regulation of bovine preadipocyte differentiation.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2476531

Jia Liu, Chicheng Ma, Yu Cheng, Minzhi Wang, Guoqing Zhao, Liwei Huang, Ruigao Song, Xi Wang, Hongxia Li

引用次数: 0

High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-07 DOI: 10.1080/01652176.2025.2486765

S Amir Kamali, Michelle Teunissen, Dirk Hendrik Nicolaas van den Broek, Elisabeth M Burgers, Guy C M Grinwis, Keita Ito, Marianna A Tryfonidou, Björn P Meij

{"title":"High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings.","authors":"S Amir Kamali, Michelle Teunissen, Dirk Hendrik Nicolaas van den Broek, Elisabeth M Burgers, Guy C M Grinwis, Keita Ito, Marianna A Tryfonidou, Björn P Meij","doi":"10.1080/01652176.2025.2486765","DOIUrl":"https://doi.org/10.1080/01652176.2025.2486765","url":null,"abstract":"The diagnosis and management of lumbosacral pain in dogs is challenging, requiring thorough examination, with MRI playing a crucial diagnostic role. This retrospective study investigates the presence of high-intensity zones (HIZ) in the dorsal annulus fibrosus (AF) of the lumbosacral region on MRI and describes the corresponding histopathological features in dogs with intervertebral disc (IVD) degeneration. T2-weighted (T2W) and T1-weighted (T1W) sagittal MRI scans were evaluated using a classification system developed in human medicine to analyze HIZ characteristics. Among 836 dogs with IVD degeneration, 57 (6.8%) exhibited T2W HIZ, with a median age of 7 years and median weight of 33.7 kg. All cases with HIZ consistently exhibited radiological degenerative lumbosacral stenosis. The most common T2W HIZ shape was round (43%), while 14% of lesions also appeared hyperintense on T1W. Histopathological analysis of 11 dorsal AF samples collected during standard-of-care decompressive surgery revealed two patterns: reactive cystic structures (3/11) and granulation tissue (8/11), with differential MRI presentation. This is the first study to document HIZ in the lumbosacral level of dogs with IVD degeneration. With this recognition, prospective analyses and their correlation with clinical presentations will be essential in determining the role and prognostic significance of HIZ.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-24 DOI: 10.1080/10495398.2025.2450364

Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

{"title":"Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.","authors":"Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2450364","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450364","url":null,"abstract":"Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets. Additionally, Madakhlasht sheep, which sometimes produce twins, and Balkhi sheep, which produce only singletons, were used as references. The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. Notably, two point mutations and a six-nucleotide insertion were found upstream of the initiation codon in twin-producing Kari ewes, potentially affecting ZP3 expression and LS. Two missense mutations (I101L in exon 1 and R408H in exon 8) were heterozygous in twin-producing Kari ewes but homozygous in other groups, correlating with LS. Protein modelling suggested that the I101L mutation alters the binding site, potentially impacting protein function. These findings indicate that ZP3 gene variations influence reproductive efficiency and LS in sheep, with specific variants serving as potential markers for selective breeding to enhance LS.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450364"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on immortalization of Mongolian sheep fibroblast cells.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-07 DOI: 10.1080/10495398.2025.2459915

Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao

{"title":"Study on immortalization of Mongolian sheep fibroblast cells.","authors":"Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao","doi":"10.1080/10495398.2025.2459915","DOIUrl":"https://doi.org/10.1080/10495398.2025.2459915","url":null,"abstract":"This study aims to establish an immortalized fibroblast cell line from Mongolian sheep. Primary Mongolian sheep fibroblasts (SSF) were isolated using tissue explant and enzymatic digestion methods, followed by microscopic observation, growth curve plotting, and karyotype analysis. The results confirmed the successful isolation of SSF. Human (hTERT) and sheep (sTERT) telomerase reverse transcriptase vectors were separately introduced into SSF, with cells passaged up to 36 generations following G418 selection. Microscopic examination and qRT-PCR results demonstrated that TERT transfection did not alter the morphology of SSF and led to stable, high levels of TERT expression (P < 0.01). Cell counting and flow cytometry revealed that TERT-transfected cells had higher viability and lower apoptosis rates compared to SSF (P < 0.05). Karyotype and soft agar colony formation assays indicated that hTERT and sTERT-transfected cells maintained normal characteristics without malignant transformation. β-galactosidase staining indicated that TERT transfection significantly reduced cellular senescence (P < 0.001). Additionally, sTERT-transfected cells exhibited higher TERT expression, enhanced viability, proliferation, and anti-senescence effects compared to hTERT-transfected cells (P < 0.05). In summary, the introduction of hTERT and sTERT effectively extends the lifespan of SSF, with sTERT demonstrating a more pronounced effect. This study provides critical evidence for preserving Mongolian sheep genetic resources and developing immortalized cell lines.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2459915"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/10495398.2025.2461176

Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao

{"title":"Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat.","authors":"Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao","doi":"10.1080/10495398.2025.2461176","DOIUrl":"https://doi.org/10.1080/10495398.2025.2461176","url":null,"abstract":"Insulin-like Growth Factor 2 mRNA-binding Protein 1 (IGF2BP1) is a candidate gene of significant interest for modulating economically important traits in livestock and poultry. The second intron of IGF2BP1 has been implicated in growth-related traits, though its precise mechanistic role remains elusive. Initial resequencing analyses in our laboratory indicated strong selective pressures on the IGF2BP1 genomic region, prompting the selection and identification of several single nucleotide polymorphisms (SNPs). Seven SNPs were mapped to the conserved region of the second intron, necessitating further investigation into their functional relevance and association with growth traits. In this study, 348 Nanjiang Yellow goats were analyzed, and the association analysis via the GLM program in SAS 9.4 identified five SNPs significantly correlated with growth traits. Notably, rs652062749(A > G) emerged as a critical locus influencing later-stage growth traits. Furthermore, strong linkage disequilibrium was observed among three SNPs, with the rs638185407 (T > A) variant markedly enhancing luciferase activity in H293T cells. Combination genotypes TTAACT, TTCCCC, and ATCACT were identified as superior for growth traits, offering theoretical insights for genetic co-breeding. This study underscores the potential utility of IGF2BP1 as a functional genetic marker in Nanjiang Yellow goat breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2461176"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and characterization of ciprofloxacin-resistant Salmonella enterica spp. isolated from food animals during 2010-2023 in South Korea.

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/01652176.2025.2473733

Md Sekendar Ali, Hee-Seung Kang, Bo-Youn Moon, Ye-Eun Heo, Min Young Kim, Ji-Hyun Choi, Yu-Jeong Hwang, Ji-In Kim, Yeon-Hee Lee, Jae-Myung Kim, Suk-Kyung Lim

{"title":"Prevalence and characterization of ciprofloxacin-resistant Salmonella enterica spp. isolated from food animals during 2010-2023 in South Korea.","authors":"Md Sekendar Ali, Hee-Seung Kang, Bo-Youn Moon, Ye-Eun Heo, Min Young Kim, Ji-Hyun Choi, Yu-Jeong Hwang, Ji-In Kim, Yeon-Hee Lee, Jae-Myung Kim, Suk-Kyung Lim","doi":"10.1080/01652176.2025.2473733","DOIUrl":"10.1080/01652176.2025.2473733","url":null,"abstract":"We isolated 6,561 Salmonella strains from food animals, cattle (n = 217), pigs (n = 1526), chickens (n = 3942), and ducks (n = 876). Isolates were evaluated for antimicrobial sensitivity, mutations in quinolone resistance determination regions (QRDRs), and plasmid-mediated quinolone resistance (PMQR) genes. Clonal relationship and genetic diversity were assessed by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Overall, 3.1% of isolates exhibited resistance to ciprofloxacin. Commonly identified mutations in QRDRs were S83F, D87N, and D87G in gyrA; T57S and S80I in parC; and L416F in parE. Furthermore, mutations differed by serotypes. In S. Albany, S83F mutation in gyrA and T57S in parC were prevalent, while in S. Kentucky, S83F and D87N in gyrA, T57S and S80I in parC; and in S. Indiana, S83F and D87G in gyrA, T57S and S80R in parC, and L416F in parE were common. Amongst PMQRs, qnrS was mainly observed in S. Albany, aac(6')-Ib-cr in S. Indiana, and qnrB1 in S. Albany. Among STs, ST198 S. Kentucky was predominant, followed by ST292 S. Albany and ST17 S. Indiana. Of 26 pulsotypes, KX1KA1 was mainly identified in S. Kentucky, AX1AA1 in S. Albany, and IX1IA1 in S. Indiana. Taken together, ciprofloxacin-resistant Salmonella can pose health hazards to humans and other animals.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2471785

Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao

{"title":"The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.","authors":"Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao","doi":"10.1080/10495398.2025.2471785","DOIUrl":"https://doi.org/10.1080/10495398.2025.2471785","url":null,"abstract":"In order to study the distribution characteristics of intestinal microbiota and antibiotic resistance genes (ARGs) in Duzang pigs after adding stems and leaves of Panax notoginseng to the feed, the characteristics of intestinal microbiota were explored by metagenomic sequencing, and 14 ARGs and 2 integrase genes were detected by qPCR. The results showed that the addition of stems and leaves of P. notoginseng increased the relative abundance of Firmicutes, Lactobacillus and Pediococcus in the cecum of Duzang pigs. A total of 10 ARGs and 2 integrase genes were detected in the cecal contents of pigs. The addition of stems and leaves of P. notoginseng reduced the relative abundance of total ARGs, ermB, tetO and tetW in the cecum of Duzang pigs. The results of network analysis showed that multiple genera were potential hosts of ARGs. The addition of stems and leaves of P. notoginseng may reduce the relative abundance of ARGs by reducing the relative abundance of genera such as Corynebacterium and Flavonifractor, thereby reducing the risk of ARGs spread. This study provides a theoretical basis for the rational use of stems and leaves of P. notoginseng to control ARGs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2471785"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key factors causing ketosis in dairy cows with low feed intake.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-04 DOI: 10.1080/10495398.2025.2487089

Xue Feng, Qi Feng, Sayed Haidar Abbas Raza, Fen Li, Yun Ma

引用次数: 0

Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats. 阴阳补充对奶山羊繁殖性能、抗氧化性和免疫力的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/10495398.2025.2450349

Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai

{"title":"Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats.","authors":"Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai","doi":"10.1080/10495398.2025.2450349","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450349","url":null,"abstract":"This study aims to explore the effects of Yin and Yang Double Supplement Compound Chinese Medicine Preparations (YYSBFF) on the reproductive performance, antioxidant levels, and immunity of dairy goats. For the experiment, 36 Alps milk goats were selected and randomly divided into an experimental group and a control group, with 18 goats in each group. The ewes in the experimental group were fed with YYSBFF for 14 d prior to breeding and farrowing. The results of the experiment showed that the estrus rate, embryo acceptance rate, and lamb birth weight in the experimental group were higher than those in the control group, and the weak lamb rate was significantly lower. Moreover, the experimental group exhibited higher levels of reproductive hormones (FSH, LH), antioxidant factors (T-SOD, GSH-Px, MDA), and immunoglobulins (IgA, IgM, IgG) compared to the control group. There were no significant differences in liver and kidney function indicators (ALT, AST, TP, ALB, CREA, UREA) between the experimental and control groups (p > .05). These findings indicate that YYSBFF can enhance the reproductive performance of dairy goats by regulating the level of sex hormones, while also improving the body's antioxidant and immune abilities.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450349"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0