IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-06-08 DOI: 10.1080/01652176.2026.2685619

Sushil Paudyal, Rajesh Neupane, Bhuwan Shrestha, Hsin-I Chiang, Ashrant Aryal

{"title":"Evaluating machine learning algorithms based on thermal imaging and milk-based parameters to identify subclinical mastitis in dairy cows.","authors":"Sushil Paudyal, Rajesh Neupane, Bhuwan Shrestha, Hsin-I Chiang, Ashrant Aryal","doi":"10.1080/01652176.2026.2685619","DOIUrl":"10.1080/01652176.2026.2685619","url":null,"abstract":"The objective was to evaluate the potential of machine learning algorithms utilizing thermal imaging and/or milk-based parameters for the identification of subclinical mastitis(SCM) in dairy cows. Holstein dairy cows (n = 194) were enrolled, and milk samples were collected from each quarter separately (n = 776 quarter-level samples) and analyzed for milk-based parameters. Four images per cow, representing all four quarters, were captured using a handheld infrared camera. Skin temperatures were extracted using Fluke Connect software, yielding 776 images for analysis. SCM was defined at the quarter level as SCC > 200,000 cells/mL, identifying 139 infected and 637 healthy quarters. The data were analyzed to test Random Forest Classifier(RFC), Logistic Regression Classifier, AdaBoost Classifier(ABC), and Naïve Bayes Classifier algorithms. The RFC models using milk features and temperature features achieved accuracy and precision scores of 0.88 and 0.88, respectively, yielding 0.90 AUC values, with high feature importance for milk lactose and SNF. Using only thermal imaging features, the ABC model yielded accuracy and precision of 0.82 and 0.67, respectively, with 0.56 AUC. We conclude that SCM prediction using machine learning algorithms is most promising when combined with thermal images and milk-based parameters. Future studies with larger datasets and refined methods for thermal image capture and analysis are warranted to validate these findings.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2685619"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13248482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148206995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk extracellular vesicles reveal insulin resistance status in peripartum dairy cows. 乳细胞外囊泡揭示围产期奶牛胰岛素抵抗状况。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-05-01 DOI: 10.1080/01652176.2026.2665485

Madhusha Prasadani, Hanno Jaakson, Priit Karis, Subhashini Muhandiram, Merike Henno, Sirje Kuusik, Katri Ling, Harinda Rajapaksha, Meelis Ots, Aneta Andronowska, Suranga Pradeep Kodithuwakku, Vallo Volke, Alireza Fazeli

{"title":"Milk extracellular vesicles reveal insulin resistance status in peripartum dairy cows.","authors":"Madhusha Prasadani, Hanno Jaakson, Priit Karis, Subhashini Muhandiram, Merike Henno, Sirje Kuusik, Katri Ling, Harinda Rajapaksha, Meelis Ots, Aneta Andronowska, Suranga Pradeep Kodithuwakku, Vallo Volke, Alireza Fazeli","doi":"10.1080/01652176.2026.2665485","DOIUrl":"10.1080/01652176.2026.2665485","url":null,"abstract":"The transition from gestation to lactation is a critical period that leads to insulin resistance (IR) in dairy cows. IR detection requires invasive sampling and laborious methods; therefore, novel and non-invasive approaches are essential. Extracellular vesicles (EVs) are nano-sized particles released by cells and are linked to disease pathophysiology. Hence, the present study aimed to explore the potential application of cow milk EVs (MEVs) in detecting IR in dairy cows. An intravenous glucose tolerance test (IVGTT) was performed (n = 12), and the cows were classified as low, moderate, or high IR based on prepartum area under the curve insulin values. MEVs were enriched using double-size exclusion chromatography and physico-chemically characterized. The results revealed that the particle diameter of MEVs was significantly higher (p ≤ 0.05) in the high-IR group compared to other groups. Out of 26 fatty acids (FAs) detected in MEVs, three long-chain FAs differed significantly in abundance (p ≤ 0.05) in high-IR group. Proteomic analysis revealed a correlation of inflammation and the IR-related proteins; Annexin A1 and Rab proteins, to IR severity. Overall, this study identified promising predictors of IR in cows, supporting the development of non-invasive biomarkers for early detection, facilitating improved herd and disease management, and reducing economic losses.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2665485"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13137739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of A SLAMF1 Gene Change That Increases Dogs' Risk of Developing Canine Atopic Dermatitis: a Plain Language Summary. 发现一种增加狗患犬特应性皮炎风险的SLAMF1基因变化：简单的语言总结。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-06-09 DOI: 10.1080/01652176.2026.2670342

Oliver P Forman, Jamie Freyer, Abigail Kerr, Julia D Labadie, Debbie J Gow, Janet Alexander

引用次数: 0

Vaccine diluent affects expression levels of immune related genes in caecal tonsils of layer chickens vaccinated with live attenuated Salmonella Typhimurium vaccine. 疫苗稀释液对接种鼠伤寒沙门氏菌减毒活疫苗的蛋鸡盲肠扁桃体免疫相关基因表达水平的影响

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-07-19 DOI: 10.1080/01652176.2026.2678598

Samiullah Khan, Andrea R McWhorter, Daniel M Andrews, Gregory J Underwood, Kapil K Chousalkar

{"title":"Vaccine diluent affects expression levels of immune related genes in caecal tonsils of layer chickens vaccinated with live attenuated Salmonella Typhimurium vaccine.","authors":"Samiullah Khan, Andrea R McWhorter, Daniel M Andrews, Gregory J Underwood, Kapil K Chousalkar","doi":"10.1080/01652176.2026.2678598","DOIUrl":"10.1080/01652176.2026.2678598","url":null,"abstract":"In this study, Salmonella Typhimurium (ST) vaccine diluent effects on caecal tonsils gene regulation in layer chicks were investigated. A live attenuated ST vaccine was reconstituted in Marek's and/or water diluents, chicks vaccinated and caecal tonsils were assessed for global gene expression at days 7 and 14 post-vaccination. Differentially expressed genes (DEGs) involved in the activation of the intestinal immune network for IgA production were upregulated earlier (e.g. day 7 post-vaccination) in the water diluent group, while in the Marek's diluent group, this pathway was enriched by the upregulated DEGs at day 14 post-vaccination. The commonly upregulated biological pathways between the two diluents were cytokine-cytokine receptor interaction, toll-like receptor signaling pathway and cytosolic DNA-sensing pathway. At day 7 post-vaccination, 28.5% and 40.2% of the significantly upregulated genes were unique to Marek's and water diluents, respectively, whereas 31.3% were common between the Marek's and water diluent groups, showing the role of the diluent in priming the vaccine for host immune system modulation. At day 14 post-vaccination, 52.7% and 15.4% of the upregulated DEGs were unique to Marek's and water diluents, respectively. Overall, the data showed that the live attenuated ST vaccine reconstituted in Marek's diluent affected the regulation of immune system-related genes in the caecal tonsils of chicks, with a difference in pattern when water was used as a diluent.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2678598"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13386580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148521619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of bleomycin in dogs treated with electrochemotherapy. 博来霉素在电疗犬体内的药代动力学。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-03-25 DOI: 10.1080/01652176.2026.2648249

Nina Milevoj, Urša Lampreht Tratar, Tina Kosjek, Mojca Kržan, Filippo Torrigiani, Gregor Serša, Maja Čemazar, Nataša Tozon

{"title":"Pharmacokinetics of bleomycin in dogs treated with electrochemotherapy.","authors":"Nina Milevoj, Urša Lampreht Tratar, Tina Kosjek, Mojca Kržan, Filippo Torrigiani, Gregor Serša, Maja Čemazar, Nataša Tozon","doi":"10.1080/01652176.2026.2648249","DOIUrl":"10.1080/01652176.2026.2648249","url":null,"abstract":"Bleomycin (BLM) is a cytotoxic antibiotic used in veterinary oncology, primarily in electrochemotherapy (ECT), a local ablative therapy where electric pulses increase drug uptake in tumours. BLM is administered intravenously or intratumourally, with the standard intravenous dose for dogs being 15,000 IU/m², followed by electric pulses 8-10 minutes later. This protocol is derived from human oncology and lacks extensive pharmacological data in dogs. We studied BLM pharmacokinetics in 29 dogs with various tumours treated with intravenous BLM and ECT between 2017 and 2023. Samples were collected from serum of 15 dogs, serum and tumours of 8 dogs, and tumours of 6 dogs. The mean volume of distribution (Vd) of BLM was 224.5 ± 75.02 ml/kg, clearance (CL) was 7.04 ± 2.05 ml/kg/min, and area under the curve (AUC) was 65.87 ± 2.11 µg·min/l. The half-life (t₁/₂) of BLM in dogs was 22.03 ± 0.88 minutes. No significant difference was found in tumour BLM concentrations between 8 minutes post-administration and 2 minutes after pulse completion. These results support the recommended 8-28-minute window for applying electric pulses following intravenous BLM administration and may indicate no need for dose adjustments based on body weight or age.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2648249"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13021021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147516267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leverage of quick-soluble gelatin microparticles in transarterial chemoembolization of canine urothelial carcinomas. 速溶明胶微粒在犬尿路上皮癌经动脉化疗栓塞中的作用。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-05-29 DOI: 10.1080/01652176.2026.2679557

Sunghoon Jeon, Gahyun Lee, Jin-Kyung Kim, Namsoon Lee, Dongwoo Chang

{"title":"Leverage of quick-soluble gelatin microparticles in transarterial chemoembolization of canine urothelial carcinomas.","authors":"Sunghoon Jeon, Gahyun Lee, Jin-Kyung Kim, Namsoon Lee, Dongwoo Chang","doi":"10.1080/01652176.2026.2679557","DOIUrl":"10.1080/01652176.2026.2679557","url":null,"abstract":"Lower urinary tract carcinomas in dogs are rare conditions with a poor prognosis. This prospective clinical study evaluated the safety, feasibility, and therapeutic efficacy of transarterial chemoembolization (TACE) using quick-soluble gelatin microparticles (QS-GMP) in 29 client-owned dogs with lower urinary tract carcinomas. Tumors primarily involved the bladder and urethra. Following selective catheterization of tumor-feeding vessels, intra-arterial carboplatin was administered prior to QS-GMP embolization. All patients underwent follow-up computed tomography (CT) scanning 4 weeks post-TACE. Tumor volume decreased in all cases, with a median reduction of 76.41% (95% confidence interval: 65.06-78.41%, p < 0.001). Partial remission was observed in 96.5% of patients; one case showed stable disease. Clinical signs, including pollakiuria, hematuria and stranguria, improved. No major complications occurred during the procedure or within two weeks after the procedure, and minor adverse effects were self-limiting. Repeat TACE was performed in 28/29 patients. The median survival time was 569 days for all patients and 534 days when non-tumor-related deaths were excluded. These findings suggest that TACE with QS-GMP may be an option for dogs with unresectable lower urinary tract carcinomas, with potential for repeated interventions and prolonged survival.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2679557"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148058193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic nanohydroxyapatite-peptide silk fibroin hydrogel induces osteogenesis in canine periodontal ligament stem cells revealed by proteomic analysis. 蛋白质组学分析显示磁性纳米羟基磷灰石肽丝素水凝胶诱导牙周韧带干细胞成骨。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-05-16 DOI: 10.1080/01652176.2026.2672558

Steven Dwi Purbantoro, Marta Scatena, Teeanutree Taephatthanasagon, Watchareewan Rodprasert, Poorichaya Somparn, Saharat Nanthawong, Jiradej Makjaroen, Pongsakorn Jantaratana, Rangsini Mahanonda, Noppadol Sa-Ard-Iam, Thanaphum Osathanon, Sirinun Pisamai Tabtieang, Chenphop Sawangmake, Sirirat Rattanapuchpong

{"title":"Magnetic nanohydroxyapatite-peptide silk fibroin hydrogel induces osteogenesis in canine periodontal ligament stem cells revealed by proteomic analysis.","authors":"Steven Dwi Purbantoro, Marta Scatena, Teeanutree Taephatthanasagon, Watchareewan Rodprasert, Poorichaya Somparn, Saharat Nanthawong, Jiradej Makjaroen, Pongsakorn Jantaratana, Rangsini Mahanonda, Noppadol Sa-Ard-Iam, Thanaphum Osathanon, Sirinun Pisamai Tabtieang, Chenphop Sawangmake, Sirirat Rattanapuchpong","doi":"10.1080/01652176.2026.2672558","DOIUrl":"10.1080/01652176.2026.2672558","url":null,"abstract":"The architecture of bone defects presents a challenge in designing scaffolds that support structural integrity and enhance osteoinduction. To address this, a noninvasive injectable silk fibroin (SF)-based hydrogel incorporating magnetic nanohydroxyapatite (MHAp) and osteogenic growth peptide (OGP) was developed. This study investigated the osteogenic differentiation potential of the hydrogel and its underlying mechanism on canine periodontal ligament stem cells (cPDLSCs). The incorporation of MHAp and/or OGP significantly enhanced osteogenic differentiation as evidenced by increased alkaline phosphatase activity, osteogenic marker expression, and biomineralization. Proteomics analysis revealed that TGF-β, BMP, and Notch signaling pathways were involved in hydrogel-induced osteogenic differentiation, which was further validated by an inhibition assay. Overall, the injectable SF hydrogel containing MHAp and OGP demonstrates strong potential for clinical application in the treatment of alveolar bone defects.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2672558"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13182179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147960528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of centrifugation-based therapeutic plasma exchange on coagulation parameters in dogs with canine visceral leishmaniasis. 离心治疗血浆交换对犬内脏利什曼病狗凝血参数的影响。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-10-14 DOI: 10.1080/01652176.2026.2706032

Suellen Rodrigues Maia, Silvano Salgueiro Geraldes, Fábio Dos Santos Nogueira, Maria Gabriela Picelli de Azevedo, Reiner Silveira de Moraes, Julia Franco Ferreira, Teresa Alessandra de Lima Núñez, Regina Kiomi Takahira, Alessandra Melchert, Adriano Sakai Okamoto, Priscylla Tatiana Chalfun Guimarães-Okamoto

{"title":"Effects of centrifugation-based therapeutic plasma exchange on coagulation parameters in dogs with canine visceral leishmaniasis.","authors":"Suellen Rodrigues Maia, Silvano Salgueiro Geraldes, Fábio Dos Santos Nogueira, Maria Gabriela Picelli de Azevedo, Reiner Silveira de Moraes, Julia Franco Ferreira, Teresa Alessandra de Lima Núñez, Regina Kiomi Takahira, Alessandra Melchert, Adriano Sakai Okamoto, Priscylla Tatiana Chalfun Guimarães-Okamoto","doi":"10.1080/01652176.2026.2706032","DOIUrl":"10.1080/01652176.2026.2706032","url":null,"abstract":"As in humans, therapeutic plasma exchange (TPE) has been investigated in veterinary medicine for managing immune-mediated diseases such as canine visceral leishmaniasis (CVL). TPE may improve clinical outcomes by removing circulating immune complexes and excess antibodies; however, its physiological effects must be understood to ensure safety. Given the scarcity of studies in animals, this study evaluated the effects of centrifugation-based TPE in dogs with CVL receiving fresh frozen plasma (FFP) as replacement fluid. Thirty-one dogs with CVL that failed to respond to conventional therapy were allocated to two groups: clinical treatment alone (n = 18) and clinical treatment plus TPE (n = 13). The TPE group underwent one daily session for three consecutive days. Coagulation profiles were assessed before and after each session. A progressive decrease in platelet counts occurred across sessions, but without significant thrombocytopenia or critical alterations in activated partial thromboplastin time (aPTT) or prothrombin time (PT), which remained within reference ranges. Transient hypocalcemia occurred after the first session and was promptly corrected with calcium gluconate supplementation. Fibrinogen levels decreased mildly without clinical evidence of bleeding. Clinical complications were minimal and effectively managed. In conclusion, TPE was well tolerated, producing predominantly transient and controllable effects on hemostasis and overall clinical homeostasis under the conditions of this study.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2706032"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13670271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High ex vivo recombination rate of African swine fever virus in porcine alveolar macrophages. 非洲猪瘟病毒在猪肺泡巨噬细胞中的高体外重组率。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-10-23 DOI: 10.1080/01652176.2026.2740247

István Mészáros, Ferenc Olasz, István Miklós, Eszter Göltl, Barbara Oláh, Zoltán Zádori

{"title":"High ex vivo recombination rate of African swine fever virus in porcine alveolar macrophages.","authors":"István Mészáros, Ferenc Olasz, István Miklós, Eszter Göltl, Barbara Oláh, Zoltán Zádori","doi":"10.1080/01652176.2026.2740247","DOIUrl":"10.1080/01652176.2026.2740247","url":null,"abstract":"Using fluorescently labeled African Swine Fever Virus (ASFV) strains, a high (~0.2) quasi-recombinant frequency (qRF3-4) was detected ex vivo during 3-4 replication cycles in naïve porcine alveolar macrophages (PAM). While co-infection and recombination frequencies remained low in PAM, they increased sharply between the second and fourth viral cycles. Infection multiplicity and synchronicity significantly influenced ASFV recombination frequency, which correlated positively with genetic distance. Crossing the ASFV/Lv17/WB/Rie1 mutants yielded a saturation curve resembling the Haldane mapping function, plateauing at ~20% recombination frequency at 160 kbp. Crossing less homologous strains (ASFV/Lv17/WB/Rie1 × ASFV/NHV/P68) reduced the recombination frequency; however, at large marker distances (140 kbp), it approached values (0.18) observed in homologous strains. A novel mathematical model estimated the average co-infection rate to be 0.47 after three viral cycles. Empirical application of the model revealed that 1 centimorgan translates to ~550-1650 bp, meaning offspring virions from a single cell undergo an average of 1.15-3.45 recombination events. This high recombination rate is consistent with in vivo observations and has critical implications for ASFV prevention and vaccination strategies. Such genomic plasticity can facilitate rapid viral evolution, potentially undermining the efficacy of live-attenuated vaccines globally.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2740247"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13700516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149240416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative validation of LC-MS, micro-bioassay, and ELISA for lincomycin quantification: application to pharmacokinetic analysis in healthy and co-infected porcine models. LC-MS、微生物测定法和ELISA法用于林可霉素定量的比较验证：在健康和合并感染猪模型药代动力学分析中的应用

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2026-12-31 Epub Date: 2026-07-25 DOI: 10.1080/01652176.2026.2708928

Syed Al Jawad Sayem, Seung-Jin Lee, Ling Gui, Md Sekendar Ali, Seung-Chun Park

{"title":"Comparative validation of LC-MS, micro-bioassay, and ELISA for lincomycin quantification: application to pharmacokinetic analysis in healthy and co-infected porcine models.","authors":"Syed Al Jawad Sayem, Seung-Jin Lee, Ling Gui, Md Sekendar Ali, Seung-Chun Park","doi":"10.1080/01652176.2026.2708928","DOIUrl":"10.1080/01652176.2026.2708928","url":null,"abstract":"This study compared liquid chromatography-mass spectrometry (LC-MS), micro-bioassay (MBa), and enzyme-linked immunosorbent assay (ELISA) for quantification of lincomycin (10 mg/kg BW) in porcine serum and evaluated lincomycin pharmacokinetic (PK) data in healthy piglets also co-infected with Actinobacillus pleuropneumoniae and Pasteurella multocida. All methods satisfied standard bioanalytical validation criteria, including low limits of detection and quantification, acceptable intra-assay imprecision and error, and strong linearity across clinically relevant concentration ranges. Serum concentrations measured by MBa and ELISA showed high correlation with LC-MS in both healthy and infected models (Pearson r ≥ 0.97, low bias on Bland-Altman analysis); they produced comparable PK parameters (Cmax, Tmax, area under the curve [AUC], clearance, and mean residence time), indicating suitability for PK/pharmacodynamic (PD)-based dose optimization. PK analysis demonstrated rapid absorption after intramuscular administration and disease-associated alterations in selected distribution and disposition parameters. AUC and clearance were nearly similar across analytical methods and largely consistent between software platforms (WinNonlin and PKSolver). Thus, lower-cost MBa and ELISA-combined with freely available PKSolver-can generate robust, decision-relevant PK/PD data for lincomycin in swine, providing a practical framework for antimicrobial dose refinement and stewardship in resource-limited veterinary and farm settings.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"46 1","pages":"2708928"},"PeriodicalIF":3.9,"publicationDate":"2026-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13403571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148594390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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