农林科学最新文献

{"title":"Regulation of PPARγ in the development of early sheep embryos in vitro.","authors":"Hengbin Yu, Chang Liu, Yue Zhang, Zhenghang Li, Wenhui Pi, Guangdong Hu","doi":"10.1016/j.theriogenology.2024.12.007","DOIUrl":"10.1016/j.theriogenology.2024.12.007","url":null,"abstract":"<p><p>Lipid metabolism plays an important role in the regulation of early embryonic development in mammals. However, the effect of lipid metabolism mediated by peroxisome proliferator-activated receptor γ (PPARγ) on the early embryonic development of sheep remains unclear. In this study, rosiglitazone (RSG), a PPARγ activator, was added to the in vitro embryo culture (IVC) medium to regulate the continuous expression of PPARγ. This study aimed to evaluate PPARγ expression during early embryonic development in sheep as well as its effects on lipid deposition, reactive oxygen species (ROS) and glutathione (GSH) levels, apoptosis and lipid metabolism-related gene expression, and embryonic development. PPARγ was not detected at 2-cell, 4-cell, 8-cell, and morula stage, while widely expressed with obvious nuclear expression features in blastocysts. Notably, treatment with 5 μM RSG in sheep parthenogenetic activated (PA) embryos significantly increased the blastocyst rate, lipid content, and GSH levels, while decreasing ROS levels. Further analysis revealed that RSG treatment upregulated the expression levels of antioxidant genes (SOD2 and CAT), anti-apoptotic gene (BCL2), and lipid metabolism-related genes (SCD-1, CD36, PLIN2, FABP3, and FABP4). Taken together, these results suggest that PPARγ plays a vital role in promoting embryonic development by enhancing lipid metabolism and reducing oxidative stress.</p>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"143-150"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different concentrations of N-acetylcysteine on the sperm quality, antioxidant enzyme activity, and antioxidant gene expression of cryopreserved goat semen.","authors":"Xiaodong Wang, Jinhong Luo, Maosheng Cao, Yonghong Ju, Qingmeng Long, Rong Yang, Quan Ji, Guangbin Zhou, Junwei Zhang, Ruiyang Li, Xiang Chen","doi":"10.1016/j.theriogenology.2024.12.009","DOIUrl":"10.1016/j.theriogenology.2024.12.009","url":null,"abstract":"<p><p>During cryopreservation, spermatozoa produce excess reactive oxygen species (ROS), which attack the plasma membrane, disrupt the physiological structure of the sperm, and ultimately decrease semen quality. This study investigated the effects of different N-acetylcysteine (NAC) concentrations on the cryopreservation of semen from Qianbei Ma goats. Semen samples were collected from five bucks with motility rates above 80 %. The treatment groups were diluted 20-fold in extenders containing 3 or 9 mM NAC and cryopreserved in liquid nitrogen, whereas the control group did not include NAC. After thawing, the sperm motility, antioxidant gene expression, enzyme activity, and cell structure were analysed. The NAC-treated groups showed improved post-thaw sperm motility. The 9 mM NAC group presented the highest catalase (CAT) and glutathione peroxidase activities, lowest ROS levels, and fewest apoptotic sperms. Moreover, the 3 mM NAC group presented the highest superoxide dismutase activity and L-cysteine levels and the lowest malondialdehyde levels. Additionally, sperm membrane integrity and mitochondrial membrane potential were significantly higher in the NAC-treated group than that in the control. Further analysis of antioxidant and apoptotic gene expression in the treated sperm revealed that the 9 mM NAC group presented significantly greater CAT and GPX4 expression than the control and 3 mM NAC groups, whereas the apoptotic genes BAX and Caspase3 were elevated in the control group compared to both the NAC groups. In summary, adding NAC to semen extenders enhanced antioxidant gene expression, increased enzyme activity, and improved post-thaw semen quality, with the 9 mM NAC treatment showing the optimal effects.</p>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"101-109"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to West Nile Virus in Wild Lagomorphs in Spanish Mediterranean Ecosystems.","authors":"Sabrina Castro-Scholten, Javier Caballero-Gómez, Daniel Bravo-Barriga, Francisco Llorente, David Cano-Terriza, Miguel Ángel Jiménez-Clavero, Débora Jiménez-Martín, Leonor Camacho-Sillero, Ignacio García-Bocanegra","doi":"10.1111/zph.13200","DOIUrl":"10.1111/zph.13200","url":null,"abstract":"<p><strong>Background: </strong>West Nile virus (WNV) is the most widely distributed mosquito-borne flavivirus. Over the past decade, its spread across Europe has raised significant concerns for both public and animal health. Although WNV exposure has been evidenced in various wild mammal species in Spain, no seroepidemiological studies have been conducted on this flavivirus in wild lagomorphs so far.</p><p><strong>Aim: </strong>This study aimed to assess WNV exposure in European wild rabbit (Oryctolagus cuniculus) and Iberian hare (Lepus granatensis) populations inhabiting Spanish Mediterranean ecosystems.</p><p><strong>Methods: </strong>Sera from 540 wild lagomorphs (399 European wild rabbit and 141 Iberian hares), from 106 hunting grounds distributed throughout Andalusia (southern Spain), were collected between the 2018/2019 and 2022/2023 hunting seasons.</p><p><strong>Results: </strong>Antibodies against flavivirus were detected by blocking enzyme-linked immunosorbent assay (bELISA) in 5.0% (27/540; 95% CI: 3.2-6.8) of the wild lagomorphs. Exposure to WNV was confirmed in 4.8% (19/394; 95% CI: 2.7-6.9) of wild rabbits and 0.7% (1/141; 95% CI: 0.0-2.1) of Iberian hares by virus microneutralisation test. Anti-WNV antibodies were found in wild lagomorphs sampled from three (2.8%) hunting grounds located in western Andalusia during the seasons 2020-2021 and 2021-2022. Remarkably, this spatiotemporal distribution overlaps with the largest outbreak of WNV in Spain. Antibodies against Usutu virus and Bagaza virus were not detected in the wild lagomorph populations analysed.</p><p><strong>Conclusions: </strong>This study constitutes the first report of WNV exposure in wild rabbit in Spain and in Iberian hare worldwide. While these species seem not play a primary role in the epidemiology of the virus, they could serve as sentinel for monitoring WNV in Iberian Mediterranean ecosystems.</p>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":" ","pages":"207-214"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porkolor: A deep learning framework for pork color classification.","authors":"Yuxian Pang, Chuchu Chen, Yuedong Yang, Delin Mo","doi":"10.1016/j.meatsci.2024.109731","DOIUrl":"10.1016/j.meatsci.2024.109731","url":null,"abstract":"<p><p>Pork color is crucial for assessing its safety and freshness, and traditional methods of observing through human eyes are inefficient and subjective. In recent years, several methods have been proposed based on computer vision and deep learning have been proposed, which can provide objective and stable evaluations. However, these methods suffer from a lack of standardized data collection methods and large-scale datasets for training, leading to poor model performance and limited generalization capabilities. Additionally, the model accuracy was limited by an absence of effective image preprocessing of background noises.To address these issues, we have designed a standardized pork image collection device and collected 1707 high-quality pork images. Base on the data, we proposed a novel deep learning model to predict the color. The framework consists of two modules: image preprocessing module and pork color classification module. The image preprocessing module uses the Segment Anything Model (SAM) to extract the pork portion and remove background noise, thereby enhancing the model's accuracy and stability. The pork color classification module uses the ResNet-101 model trained with a patch-based training strategy as the backbone. As a result, the model achieved a classification accuracy of 91.50 % on our high quality dataset and 89.00 % on the external validation dataset. The Porkolor online application is freely available at https://bio-web1.nscc-gz.cn/app/Porkolor.</p>","PeriodicalId":389,"journal":{"name":"Meat Science","volume":"221 ","pages":"109731"},"PeriodicalIF":7.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of coagulase-negative staphylococci on aroma generation of fermented sausage.","authors":"Juan Chen, Shenmiao Li, Yaqiu Lin, Fidel Toldrá, Xiaonan Lu","doi":"10.1016/j.meatsci.2024.109730","DOIUrl":"10.1016/j.meatsci.2024.109730","url":null,"abstract":"<p><p>Fermented sausages are popular meat products with many different varieties. The aroma of fermented sausages depends on the metabolic activities of microbiota, mainly involving lactic acid bacteria and catalase-positive cocci, the group of coagulase-negative staphylococci (CNS) in particular. Regarding staphylococci, this work elucidated their generation of aroma precursors from hydrolase, metabolic activities contributing to aroma development, antioxidant effects that improve aroma via preventing excessive lipid oxidation. The metabolic pathways of staphylococci that play a role in aroma formation involve carbohydrate fermentation, amino acid degradation, fatty acid β-oxidation, and esterase activities. Their antioxidant activities are associated with superoxidase dismutase and catalase activities, as well as the production of antioxidant peptides. Processing conditions may influence CNS communities and affect aroma characteristics of fermented sausages. Implementation of genome sequencing and editing to select and customize CNS with specific biosynthetic metabolic pathways was proposed forward, offering a great potential for enhancing aroma development during sausage fermentation.</p>","PeriodicalId":389,"journal":{"name":"Meat Science","volume":"221 ","pages":"109730"},"PeriodicalIF":7.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered expression of systemic and ovarian factors involved in the recruitment and activation of immune cells in bovine cystic ovarian disease.","authors":"Lucas Etchevers, Pablo Uriel Díaz, María Sol Renna, Ulises Sebastian Notaro, Fernanda Mariel Rodriguez, Hugo Hector Ortega, Natalia Raquel Salvetti, Ayelén Noelia Amweg","doi":"10.1016/j.theriogenology.2024.12.006","DOIUrl":"10.1016/j.theriogenology.2024.12.006","url":null,"abstract":"<p><p>Despite the involvement of both immune cells and soluble factors in the etiopathogenesis of cystic ovarian disease (COD), the precise interplay between these components in this reproductive condition remains unclear. Based on this, in the present study we aimed to evaluate the expression of key molecules involved both locally and systemically in the recruitment, infiltration and activation of immune cells in Holstein cows diagnosed with follicular cysts (N = 5). For this, animals from commercial dairy farms located in the milk-productive region of Santa Fe, Argentina were included after their diagnosis were confirmed by ultrasonographic examination and hormonal evaluation. Healthy animals in proestrus, after G6G-Ovsynch hormonal synchronization, were used as controls (N = 5). Protein expression of adhesion molecules, along with mRNA expression of chemokine receptors and cytokines were evaluated. In peripheral blood mononuclear cells, gene expression analysis of inflammatory markers showed that TNF and TGFB1 mRNA expression was significantly lower in COD animals. Also, immunohistochemical analysis revealed that expression of vascular cell adhesion molecule 1 and platelet endothelial cell adhesion molecule was lower in endothelial cells of medullary blood vessels in the COD group. These changes support the hypothesis that alterations in the expression patterns of immune factors, both at systemic and local level, could contribute to the etiopathogenesis of this anovulatory condition.</p>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"92-100"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and Molecular Detection of Toxoplasma gondii in Wild Boars (Sus scrofa) in Khuzestan Province, Iran.","authors":"Mohammad Javad Boozhmehrani, Mohammad Hossein Feiz-Haddad, Mehdi Tavalla, Mohammad Nouri, Seyed Morteza Ghoreishi","doi":"10.1111/zph.13195","DOIUrl":"10.1111/zph.13195","url":null,"abstract":"<p><strong>Introduction: </strong>Toxoplasma gondii is a parasitic protozoan capable of infecting warm-blooded animals, including humans, and is the causative agent of toxoplasmosis. Wild boars (Sus scrofa) serve as reservoirs for zoonotic pathogens like T. gondii, which can cause disease in humans if transmitted. This study aimed to assess the seroprevalence and molecular prevalence of T. gondii in wild boars in Khuzestan Province, Iran.</p><p><strong>Methods: </strong>Seroprevalence of T. gondii antibodies was assessed using enzyme-linked immunosorbent assay (ELISA), employing a commercial kit (Calbiotech), whereas molecular detection was carried out through polymerase chain reaction (PCR) targeting the B1 gene of T. gondii on tissue samples (tongue, muscle, diaphragm and heart). Thirty wild boars were sampled from the regions of Shush, Shushtar, Dezful and Abadan, and both blood and tissue samples were analysed.</p><p><strong>Results: </strong>In this study, the seroprevalence of T. gondii antibodies was found to be 83.3% (25/30) among wild boar serum samples using ELISA. Molecular detection through PCR identified T. gondii DNA in 46.7% (14/30) of tissue samples, with the highest detection rates in tongue tissues (64%), followed by muscle (21%) and diaphragm (14%). No positive results were found in heart samples, and no animal had multiple tissues testing positive. All PCR-positive cases corresponded with positive ELISA results, and a statistically significant difference was observed in parasite prevalence across different tissues (p = 0.002).</p><p><strong>Conclusion: </strong>Although consumption of wild boar meat is banned in Iran, illegal hunting and consumption remain a concern. The high prevalence of T. gondii in wild boars poses a potential risk for transmission through the illegal consumption of undercooked or raw meat. This study highlights the need for public health interventions to control the illegal trade of wild boar meat and reduce the risk of toxoplasmosis transmission. Further research is recommended to investigate T. gondii distribution in other tissues, including the brain, and to better understand the parasite's epidemiology in this region.</p>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":" ","pages":"166-173"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin protects bovine oocyte from βHB-induced oxidative stress through the Nrf2 pathway.","authors":"Kaiyan Zhang, Jing Guo, Simin Wang, Changguo Min, Jun Wang, Hongyu Liu, Yi Fang, He Ding, Jing Zhao, Xin Ma, Wenfa Lu","doi":"10.1016/j.theriogenology.2024.11.025","DOIUrl":"10.1016/j.theriogenology.2024.11.025","url":null,"abstract":"<p><p>Accumulation of ketone bodies in the blood or tissues can trigger ketosis, exerting detrimental effects on bovine oocytes maturation. Exposure to its primary component, β-hydroxybutyric acid (βHB), disrupts mitochondrial function, culminating in the excessive buildup of reactive oxygen species (ROS) and subsequent initiation of apoptosis in oocytes. These ultimately result in poor oocyte quality. Melatonin, recognized for its endogenous antioxidant properties, is capable of mitigating ROS levels and enhancing the expression of antioxidant enzymes. In this study, we explored the protective effects of melatonin on the damages induced by βHB. Melatonin was added at a concentration of 10^-9 M to the culture medium on bovine oocytes. Parameters including first polar body extrusion rate, mitochondrial membrane potential, ROS, cell apoptosis were assessed. Results showed that melatonin could restore bovine oocyte maturation rate, enhance mitochondrial function, reduce cell apoptosis rate, and mitigate oxidative stress levels. Notably, Nrf2 signaling pathway inhibitor ML385 significantly attenuated the protective effects of melatonin on oxidative stress induced by βHB exposure. In summary, our study demonstrates that melatonin can protect oocytes from oxidative stress induced by βHB exposure, with indications that this protective mechanism may be mediated through the Nrf2 pathway.</p>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"64-72"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial effects of EGCG on boar sperm quality during liquid storage at 4 °C are mediated by DRD2 receptor.","authors":"Lirui Wang, Manyi Xiong, Sisi Li, Sheng Ma, Shengyao Jiang, Haolei Wang, Jian Zhang, Xinhong Li","doi":"10.1016/j.theriogenology.2024.12.019","DOIUrl":"10.1016/j.theriogenology.2024.12.019","url":null,"abstract":"<p><p>Epigallocatechin gallate (EGCG), a natural antioxidant, plays a vital role in modulating sperm function, yet its protective impact on boar spermatozoa during liquid preservation at 4 °C remains elusive. This study aimed to investigate the beneficial effects of EGCG on boar semen preservation, and elucidate the potential mechanism. Multiple parameters including sperm quality, anti-oxidative status, protein phosphorylation levels, membrane receptor and cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signaling pathways were analyzed using computer-assisted semen analysis system, Western blot and molecular docking techniques. Results revealed that supplementation with EGCG, particularly with 10 μg/mL, significantly increased sperm motility, acrosome integrity, mitochondrial membrane potential and intracellular ATP content. Moreover, EGCG enhanced the antioxidant defenses of sperm through eliminating excessive reactive oxygen species. Intriguingly, the antioxidant property of EGCG partly prevented protein dephosphorylation, thereby indirectly enhancing protein phosphorylation. Additionally, the dopamine receptor (DRD2) was detected in boar spermatozoa and inhibition of DRD2 greatly prevented EGCG-caused enhancement of protein phosphorylation levels and sperm motility, suggesting the role of DRD2 in regulation of the beneficial effects of EGCG. Molecular docking results indicated that EGCG has favorable binding interactions with the active sites of DRD2, involving crucial hydrogen bonding and hydrophobic interactions, further suggesting that EGCG might directly interact with DRD2, mediate protein phosphorylation via activating the DRD2/cAMP/PKA pathway and thus boost sperm motility. The present study is the first to explore the interacting cell-surface receptor of EGCG on boar sperm and provides comprehensive insights into the protective mechanism of EGCG during hypothermic liquid storage.</p>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"234 ","pages":"174-185"},"PeriodicalIF":2.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the flavor contribution, mechanisms of action, and future trends of coagulase-negative staphylococci in fermented meat products: A review.","authors":"Yuhang Fan, Iftikhar Hussain Badar, Qian Liu, Xiufang Xia, Qian Chen, Baohua Kong, Fangda Sun","doi":"10.1016/j.meatsci.2024.109732","DOIUrl":"10.1016/j.meatsci.2024.109732","url":null,"abstract":"<p><p>During fermentation, meat is pre-treated and cured to cultivate a diverse microflora, resulting in fermented meat products with distinctive flavors. Coagulase-negative staphylococcus holds a crucial role in all fermented meat products, contributing to the breakdown of proteins, carbohydrates, and fats, and the creation of flavor compounds. Fermentation technology has important research value and significance in fermented meat products. The optimization and improvement of flavor by CNS can be achieved by regulating the fermentation environment, initial microflora and processing conditions. The review explores the ways in which coagulase-negative staphylococci contribute to the flavors in fermented meat products. The mechanism of flavor substance formation and means of regulation in coagulase-negative staphylococci were also investigated. The review concludes by summarizing future development trends and drawing conclusions.</p>","PeriodicalId":389,"journal":{"name":"Meat Science","volume":"221 ","pages":"109732"},"PeriodicalIF":7.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}