IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-13 DOI: 10.1080/10495398.2025.2463995

Ioannis Brouklogiannis, Konstantinos C Mountzouris

{"title":"Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance.","authors":"Ioannis Brouklogiannis, Konstantinos C Mountzouris","doi":"10.1080/10495398.2025.2463995","DOIUrl":"https://doi.org/10.1080/10495398.2025.2463995","url":null,"abstract":"The study assessed the inclusion level effects of a phytogenic blend (PB) on performance and critical molecular biomarkers related to detoxification (Aryl hydrocarbon receptor; AhR) and antioxidant (Nuclear factor-erythroid 2-related factor 2; Nrf2) responses in layers' ovary and liver. Layers (n = 385; 21-week-old; Hy-Line Brown) were allotted to 5 treatments with 7 replicates of 11 hens each, for a 12-week feeding trial. Treatments were: control (CON) without PB or supplementation with PB at 250 (PB250), 750 (PB750), 1000 (PB1000) and 1500 mg/kg diet (PB1500), respectively. Performance was determined weekly for the 12-week experimental period. At the 6th and 12th experimental week ovarian and liver samples were collected for gene expression analysis. Increasing PB inclusion level, improved linearly and quadratically overall laying rate, egg mass and FCR, with optimal (P ≤ 0.001) performance notable in the PB750 compared to CON. The nutrigenomic analysis revealed that PB inclusion resulted (P ≤ 0.05) in consistent beneficial modulation of the AhR/Nrf2 pathway-related genes assessed at the 6th and 12th experimental week, both in the ovary and the liver of laying hens. In conclusion, PB beneficially modulated the ovarian and hepatic adaptive cytoprotection and supported the laying performance improvements recorded, with PB750 displaying the optimal benefits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2463995"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets. 伊沙提斯根多糖通过促进仔猪肠道健康和调节肠道微生物群来改善断奶后腹泻。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-03 DOI: 10.1080/01652176.2024.2447600

Miao Zhou, Zenghao Yan, Deqin Wang, Chuyuan Li, Leli Wang, Rui Li, Jie Yin, Yulong Yin

{"title":"Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets.","authors":"Miao Zhou, Zenghao Yan, Deqin Wang, Chuyuan Li, Leli Wang, Rui Li, Jie Yin, Yulong Yin","doi":"10.1080/01652176.2024.2447600","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447600","url":null,"abstract":"This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores (p < 0.01). Notably, the serum levels of immunoglobulin A and immunoglobulin G increased linearly and quadratically (p < 0.01), while immunoglobulin M also showed a linear increase (p < 0.05) in IRP-fed piglets. The secretory immunoglobulin A levels in ileal contents were significantly higher compared to control piglets (p < 0.01). Key intestinal health parameters, including villus height, villus height-to-crypt depth ratio, and goblet cell numbers, showed linear and quadratic increases in both the jejunum and ileum (p < 0.05), while crypt depth decreased significantly (p < 0.01). Additionally, the expression of IL-10, ZO-1, occludin, and mucin2 was upregulated linearly and quadratically in IRP-fed piglets (p < 0.05). In cultured IPEC-J2 cells, ZO-1 and occludin expression levels significantly increased upon exposure to 400 μg/mL IRP (p < 0.01). Furthermore, the relative abundances of Escherichia coli, Ralstonia pickettii, and Desulfovibrio fairfieldensis decreased linearly with increasing dietary IRP concentration. In conclusion, IRP shows promise as an effective dietary supplement for mitigating diarrhea and enhancing intestinal health in early weaned piglets.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methodological rigour and reporting quality of the literature on wildlife rescue, rehabilitation, and release: a global systematic review. 野生动物救援、康复和释放文献的方法严谨性和报告质量：一项全球系统综述。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-07 DOI: 10.1080/01652176.2025.2478138

Gloeta N Massie, Louis J Backstrom, Daniel P Holland, Mandy B A Paterson, Richard A Fuller

{"title":"Methodological rigour and reporting quality of the literature on wildlife rescue, rehabilitation, and release: a global systematic review.","authors":"Gloeta N Massie, Louis J Backstrom, Daniel P Holland, Mandy B A Paterson, Richard A Fuller","doi":"10.1080/01652176.2025.2478138","DOIUrl":"https://doi.org/10.1080/01652176.2025.2478138","url":null,"abstract":"Wildlife rescue, rehabilitation, and release is a global practice with a broad body of scientific literature; nonetheless, no studies have assessed and quantified the methodological rigour and reporting quality of this literature. In this PRISMA systematic review, we assessed and quantified the reporting of controls, randomisation, blinding, experimental animal data, and housing and husbandry data in 152 primary studies on wildlife rescue, rehabilitation, and release published between 1980 and 2021. We then tested for associations between reporting and study characteristics. Of the 152 reviewed studies, one study reported a control, randomisation, and blinding; 17 studies reported species, age, sex, weight, and body condition; and 14 studies reported housing size, housing location, type of food, provision of water, and provision of enrichment. No study reported all 13 of these elements. Studies published in veterinary-focused journals reported lower methodological rigour and had lower reporting quality than studies published in other types of journals. Studies on mammals had higher reporting quality than studies on birds and on reptiles, and studies that included the word \"welfare\" had higher reporting quality than studies that did not. The overall low methodological rigour and reporting quality of the literature limits study replicability and applicability and impedes meta-analyses.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples. 使用氮同位素分馏作为猪血液和毛发样本饲料转化效率的生物标志物。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI: 10.1080/10495398.2025.2473494

Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki

{"title":"The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples.","authors":"Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki","doi":"10.1080/10495398.2025.2473494","DOIUrl":"https://doi.org/10.1080/10495398.2025.2473494","url":null,"abstract":"This study aimed to 1) Establish relationships between feed conversion efficiency (FCE; live weight gain/intake) and several biomarkers in pigs using blood and hair samples, and 2) Investigate the relative FCE performance of pigs from maternal vs. terminal genetic lines fed high vs. low energy diets. 80 male pigs (Large White x Landrace) were fed for 56 days. The terminal genetic line and pigs fed the high energy diet had 5% and 15% higher FCE than the maternal genetic line and pigs fed the low energy diet, respectively. Blood nitrogen isotopic fractionation (Δ15N; animal δ15N - feed δ15N) explained 34% more variation of FCE, compared with the blood insulin‑like growth factor‑1 (IGF‑1). The turnover rate of δ15N in plasma was faster than δ15N in blood, suggesting that blood and plasma δ15N can predict long‑term versus short‑term FCE changes. Pigs fed the high energy diets showed 13% higher live weight gain, 16% greater high standard carcass weight, and 38% higher carcass backfat than those on low‑energy diets. In conclusion, Δ15N is a more effective FCE biomarker for pigs compared to IGF‑1. Hair samples were less predictive of FCE than blood‑derived biomarkers, suggesting further refinement in the methodology of harvesting hair samples.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2473494"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between the expression levels of myogenic regulatory factor genes and carcass characteristics in Kivircik and Hungarian Merino lambs. 基维尔西克和匈牙利美利奴羔羊产肌调节因子基因表达水平与胴体性状的关系。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-23 DOI: 10.1080/10495398.2025.2479690

Korhan Arslan, Fadime Daldaban, Hulya Yalcintan, Pembe Dilara Kecici, Bekir Ozturk, Bulent Ekiz, Bilal Akyuz

{"title":"Relationship between the expression levels of myogenic regulatory factor genes and carcass characteristics in Kivircik and Hungarian Merino lambs.","authors":"Korhan Arslan, Fadime Daldaban, Hulya Yalcintan, Pembe Dilara Kecici, Bekir Ozturk, Bulent Ekiz, Bilal Akyuz","doi":"10.1080/10495398.2025.2479690","DOIUrl":"https://doi.org/10.1080/10495398.2025.2479690","url":null,"abstract":"This study aimed to investigate the expression profiles of the myogenic regulatory genes MYOD1, MYOG, MYF5, MYF6, and MSTN in longissimus dorsi muscle, as well as the correlation of the expression levels of these genes with carcass characteristics and growth performance in the Kivircik and Hungarian Merino sheep breeds. The expression levels of the MYF5, MYF6, and MYOG genes were found to be significantly correlated with the rib proportion, the expression level of the MYOG gene was identified as being the main determinant of variations in the rib proportion in the Kivircik lambs. The regression analysis results revealed that the expression levels of the MYF5 and MSTN genes played an essential role in determining the cold carcass dressing percentage in Hungarian Merino lambs. Further, as a result of the regression analysis, the model including the expression level of the MYF6 gene demonstrated that this gene could be responsible for 36.4% of the differences observed in cold carcass weight. In conclusion, the findings of this study suggest that the expression levels of the MYF5, MYF6, and MYOG genes were associated with various carcass traits, particularly in the Kivircik breed, and these genes hold potential as markers for enhancing breed productivity.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2479690"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic network of infectious bronchitis virus: exploring the impact of migratory birds on viral clustering, evolution, and recombination. 传染性支气管炎病毒的系统发育网络：探索候鸟对病毒聚集、进化和重组的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-14 DOI: 10.1080/01652176.2025.2465570

Yu-Chen Tai, Geng-Ming Hu, Chi-Ming Chen

{"title":"Phylogenetic network of infectious bronchitis virus: exploring the impact of migratory birds on viral clustering, evolution, and recombination.","authors":"Yu-Chen Tai, Geng-Ming Hu, Chi-Ming Chen","doi":"10.1080/01652176.2025.2465570","DOIUrl":"10.1080/01652176.2025.2465570","url":null,"abstract":"Infectious bronchitis virus (IBV) presents a major threat to global poultry production, necessitating a thorough understanding of its evolutionary relationships for effective control measures. This study presents a novel distance-based Minimum Span Clustering (MSClustering) method to cluster 311 IBV strains, with a comparison of its results to the established IBV classification. Phylogenetic network and recombination analyses were employed to investigate IBV evolutionary relationships and transmission pathways. The phylogenetic network revealed distinct clusters reflecting relationships between IBV strains. Importantly, these network patterns, combined with recombination event analysis, suggest an unrecognized role for migratory birds in IBV dissemination, highlighting potential transmission pathways beyond established poultry trade routes. These findings contribute to advancing our understanding of IBV evolution and support the development of targeted strategies for controlling viral outbreaks in poultry populations. While statistical limitations may affect threshold estimation for smaller networks, our MSClustering method significantly accelerates processing speeds-approximately 100,000 times faster than PhyML when analyzing the dataset-enabling comprehensive-scale phylogenetic analysis of viruses.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets. 添加葡萄糖氧化酶作为抗生素替代品可减轻断奶仔猪腹泻，改善肠道健康。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-01 DOI: 10.1080/01652176.2024.2447592

Dan Chen, Jiaming Chen, Zemin Dong, Qingyun Cao, Hui Ye, Dingyuan Feng, Changming Zhang, Jianjun Zuo, Weiwei Wang

{"title":"Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets.","authors":"Dan Chen, Jiaming Chen, Zemin Dong, Qingyun Cao, Hui Ye, Dingyuan Feng, Changming Zhang, Jianjun Zuo, Weiwei Wang","doi":"10.1080/01652176.2024.2447592","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447592","url":null,"abstract":"Weaning stress-induced diarrhea is a serious issue in pig production. This study aimed to evaluate the potential of glucose oxidase (GOD) as an antibiotic substitute to alleviate diarrhea and improve gut health in weaned piglets. According to a randomized complete block design, 250 piglets weaned around 21 d of age were allocated into 5 groups (5 replicates/group), which received a basal diet without or with supplemental 200 mg/kg antibiotic, 500, 1000 and 2000 U/kg GOD, respectively. Dietary treatments did not affect (p > 0.05) growth performance of piglets. However, supplemental all doses of GOD were equivalent or superior to antibiotic to reduce (p < 0.05) diarrhea as well as increase (p < 0.05) thymus index, hepatic and colonic antioxidant properties. GOD addition at 1000 and 2000 U/kg reduced (p < 0.05) cecal and rectal pH value, respectively. They also displayed similar or superior efficacy to antibiotic in improving (p < 0.05) duodenal and jejunal morphology along with certain tight junction proteins expression of jejunum and colon. Collectively, GOD represents an antibiotic alternative to reduce diarrhea of weaned piglets through associating with ameliorations of intestinal structure and functions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots. 西班牙饲养场牛呼吸道疾病（BRD）感染动物中多重耐药多杀性巴氏杆菌分离株中与抗菌素耐药性相关的整合和共轭元件

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-08 DOI: 10.1080/01652176.2025.2474220

Carlos Serna, Johan Manuel Calderón Bernal, Laura Torre-Fuentes, Ángel García Muñoz, Alberto Díez Guerrier, Marta Hernández, José Francisco Fernández-Garayzábal, Ana Isabel Vela, Dolores Cid, Julio Alvarez

{"title":"Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots.","authors":"Carlos Serna, Johan Manuel Calderón Bernal, Laura Torre-Fuentes, Ángel García Muñoz, Alberto Díez Guerrier, Marta Hernández, José Francisco Fernández-Garayzábal, Ana Isabel Vela, Dolores Cid, Julio Alvarez","doi":"10.1080/01652176.2025.2474220","DOIUrl":"10.1080/01652176.2025.2474220","url":null,"abstract":"The emergence of multidrug-resistance (MDR) in Pasteurella multocida, a major contributor to bovine respiratory disease (BRD) is being increasingly reported, often linked to the carriage of antimicrobial resistance genes (ARGs) on integrative and conjugative elements (ICEs). The resistance phenotype for 19 antimicrobials was determined using broth microdilution in 75 Pasteurella multocida isolates from healthy and BRD-affected cattle from five feedlots. The genomes of 32 isolates were sequenced to identify ARG) and mobile genetic elements (MGEs) and assess their genetic diversity. MDR isolates (with phenotypic resistance to aminoglycosides, macrolides, fluoroquinolones and/or tetracyclines) were primarily found among BRD-affected compared to healthy animals. Non-susceptible isolates, belonging to ST79 and ST13, harbored point mutations and four to nine ARGs, including rarely reported mechanisms in Europe (mph(E), msr(E) and aadA31 ARGs and newly described mutations in the gyrA/parC genes). All ARGs were linked to the presence of MGEs including two ICEs, Tn7407 and the novel Tn7809, a prophage and a putative composite transposon. Clonally related isolates were found in different batches from the same feedlot, suggesting maintenance of MDR strains. Our findings demonstrate the diverse genetic basis of AMR in P. multocida from BRD-affected cattle in Spain, emphasizing the role of MGEs in the ARG dissemination.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The secretomes of bovine mammary epithelial cell subpopulations differentially modulate macrophage function. 牛乳腺上皮细胞亚群分泌组差异调节巨噬细胞功能。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-08 DOI: 10.1080/01652176.2025.2463338

Nikola Danev, Rebecca M Harman, Anja S Sipka, Leanne Oliveira, Lucas Huntimer, Gerlinde R Van de Walle

{"title":"The secretomes of bovine mammary epithelial cell subpopulations differentially modulate macrophage function.","authors":"Nikola Danev, Rebecca M Harman, Anja S Sipka, Leanne Oliveira, Lucas Huntimer, Gerlinde R Van de Walle","doi":"10.1080/01652176.2025.2463338","DOIUrl":"10.1080/01652176.2025.2463338","url":null,"abstract":"Bovine mammosphere-derived epithelial cell (MDEC) cultures are heterogeneous and enriched for stem and progenitor cells. We previously reported that the bovine MDEC secretome, comprised of all bioactive factors secreted by the cells, displays regenerative properties, exerts antimicrobial effects, and modulates neutrophil activity, positioning it as a promising non-antibiotic biologic therapy for infectious diseases important to the dairy industry, like mastitis. Mastitis is defined as inflammation of the udder, and it is typically caused by bacterial infection. The effect of the MDEC secretome on macrophages, a first line of defense against bacterial infections in the udder, is unknown and could impact the utility of the secretome as a therapy for mastitis. To address this, we isolated bovine monocytes from peripheral blood and maintained them as an unpolarized (M0) population or polarized them into M1 or M2 phenotypes. Macrophages cultured with the secretome of bovine MDECs were assessed for their ability to phagocytose labeled bacterial particles and accumulate reactive oxygen species (ROS). We used single-cell RNA sequencing (scRNA-seq) and fluorescence-activated cell sorting (FACS) to isolate a subpopulation of MDECs that exert enhanced effects on macrophages. We found that the secretome of MDECs that do not express cluster of differentiation (CD) 73, a cell surface enzyme used as a marker for mesenchymal stromal cells, most strongly increased macrophage phagocytosis and ROS accumulation. These findings will help optimize the generation of the bovine MDEC secretome as a suitable treatment option for mastitis.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11809179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal^® tissue sealer in pond sliders (Trachemys scripta): impacts on procedure time, surgical access, and recovery time according to follicular stage. 传统与电刀卵巢输卵管切除术与池塘滑块（Trachemys scripta）中的EnSeal®组织密封器的比较：根据卵泡分期对手术时间、手术通路和恢复时间的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI: 10.1080/01652176.2025.2473743

Alessandro Vetere, Michela Ablondi, Martina Gavezzoli, Francesco Di Ianni

{"title":"Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal® tissue sealer in pond sliders (Trachemys scripta): impacts on procedure time, surgical access, and recovery time according to follicular stage.","authors":"Alessandro Vetere, Michela Ablondi, Martina Gavezzoli, Francesco Di Ianni","doi":"10.1080/01652176.2025.2473743","DOIUrl":"10.1080/01652176.2025.2473743","url":null,"abstract":"Pond sliders (Trachemys scripta) is a common pet and invasive species in many countries. Its ability to thrive and reproduce in Mediterranean climates necessitates population control to prevent overpopulation. Ovariectomy is considered as a preventive strategy to limit reproduction and protect local biodiversity. This study compares two spaying techniques in Trachemys scripta: electrosurgery using the EnSeal® tissue sealer (group E) and a conventional approach with manual ligation of blood vessels (group C). Statistical tests evaluated procedural differences, including ANOVA for duration (factoring in follicular stage, surgery type, and their interaction) and Chi-squared tests for the likelihood of single surgical access via the prefemoral fossa and post-surgical feeding. Pearson's Chi-squared test with Yates' correction was used to assess complications by surgery type and follicular stage. ANOVA assessed the effect of follicular stage on surgery time, with follicles classified as Class I (n = 17), Class II (n = 12), and Class III (n = 7). Both follicular stage and surgery type significantly impacted surgery time, while their interaction was not significant, indicating independent effects. The likelihood of single-access procedures was significantly linked to surgery type, with EnSeal® procedures being faster and associated with earlier post-surgical feeding. Complications were not significantly linked to surgery type or follicular stage.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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