IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/01652176.2025.2475989

Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul

{"title":"Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle.","authors":"Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul","doi":"10.1080/01652176.2025.2475989","DOIUrl":"10.1080/01652176.2025.2475989","url":null,"abstract":"Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic insights into Mycobacterium orygis infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle. 转录组学对牛羊分枝杆菌感染相关的肺肉芽肿揭示了多细胞免疫网络和结核病生物标志物。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-05-27 DOI: 10.1080/01652176.2025.2509503

Rishi Kumar, Sripratyusha Gandham, Vinay Bhaskar, Manas Ranjan Praharaj, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey

{"title":"Transcriptomic insights into Mycobacterium orygis infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle.","authors":"Rishi Kumar, Sripratyusha Gandham, Vinay Bhaskar, Manas Ranjan Praharaj, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey","doi":"10.1080/01652176.2025.2509503","DOIUrl":"10.1080/01652176.2025.2509503","url":null,"abstract":"Mycobacterium orygis, a member of the Mycobacterium tuberculosis complex (MTBC), has emerged as a significant contributor to tuberculosis (TB) in cattle, wildlife, and humans. However, understanding about its pathogenesis and severity is limited, compounded by the lack of reliable TB biomarkers in cattle. This study delves into the comparative pathology and transcriptomic landscape of pulmonary granulomas in cattle naturally infected with M. orygis, using high-throughput RNA sequencing. Histopathological analysis revealed extensive, multistage granulomatous, necrotic, and cavitary lesions, indicative of severe lung pathology induced by M. orygis. Transcriptomic profiling highlighted numerous differentially expressed genes and dysregulated pathways related to immune response modulation and extracellular matrix remodelling. Additionally, cell type enrichment analysis provided insights into the multicellularity of the granulomatous niche, emphasising complex cell-cell interactions within TB granulomas. Via comparative transcriptomics leveraging publicly available bovine and human TB omics datasets, 14 key immunomodulators (SOD2, IL1α/β, IL15, IL18, CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CCL8/MCP-2, CCL20/MIP-3α, CXCL2/MIP-2, CXCL10/IP-10, CXCL11, and IFN-γ) were identified as potential biomarkers for active TB in cattle. These findings significantly advance our understanding of M. orygis pathogenesis in bovine TB and highlight potential targets for the development of diagnostic tools for managing and controlling the disease.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-19"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization and insights into the belted coat pattern of a local, reconstituted pig population. 基因组表征和见解到一个地方，重组猪群的带状被毛模式。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/10495398.2025.2515462

Samira Giovannini, Maria Giuseppina Strillacci, Raffaella Milanesi, Caterina Altissimi, Massimo Biagetti, Francesca Maria Sarti

{"title":"Genomic characterization and insights into the belted coat pattern of a local, reconstituted pig population.","authors":"Samira Giovannini, Maria Giuseppina Strillacci, Raffaella Milanesi, Caterina Altissimi, Massimo Biagetti, Francesca Maria Sarti","doi":"10.1080/10495398.2025.2515462","DOIUrl":"https://doi.org/10.1080/10495398.2025.2515462","url":null,"abstract":"The Cinghiato pig population originates from a breeding project aimed at reconstituting an extinct local swine breed, historically depicted in frescoes in Umbria, Central Italy. The selection strategy employed a reconstruction breeding program, choosing mating pairs based on the unique coat phenotype represented in these artworks. Unlike the traditional Cinta Senese breed, Cinghiato pigs exhibit a white belt encircling the trunk, while their forelimbs remain black. This study explores the genetic background of the belted coat pattern observed in the heterogeneous reconstituted population. Twenty-two pigs were genotyped using the Porcine GGP 80K SNP BeadChip. Genetic analyses were conducted to assess population structure and diversity, with comparisons made to other Italian pig breeds and wild boars. Findings reveal moderate genetic diversity within the Cinghiato population. Runs of Homozygosity patterns suggest historical inbreeding events. Moreover, several genomic regions were associated with traits relevant to niche pork production, including feed intake, leg conformation, and fat deposition. Polymorphisms were detected in 10 coat color-related genes (KIT, MC1R, ASIP, EDNRB, KITLG, MITF, OCA2, PAX3, SOX10, and TYRP1). Although some candidate variants were identified, this preliminary study highlights the need for further research to clarify the genetic mechanisms underlying the phenotypic variability of belted coat patterns.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2515462"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eucommia ulmoides and its inhibitory effects on prevotella in piglet gut microbiome through metagenomic and metabolomic analysis. 杜仲及其对仔猪肠道微生物群普氏菌的抑制作用。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/10495398.2025.2503753

Shanshan Han, Qiang Zhang, Hongyan Zhang, Jian Ma

{"title":"Eucommia ulmoides and its inhibitory effects on prevotella in piglet gut microbiome through metagenomic and metabolomic analysis.","authors":"Shanshan Han, Qiang Zhang, Hongyan Zhang, Jian Ma","doi":"10.1080/10495398.2025.2503753","DOIUrl":"https://doi.org/10.1080/10495398.2025.2503753","url":null,"abstract":"Eucommia ulmoides (EU) is a traditional medicinal plant widely cultivated across China. The combination of EU and feed significantly affects the growth performance, intestinal microbiota composition, and metabolic characteristics of weaned piglets. Forty Landrace x Yorkshire piglets were randomly assigned to four groups: a control group receiving a basal diet, three treatment groups receiving a basal diet supplemented with EU and EU with mix energy (EU+ME), and EU with high protein and energy (EU+HPE), respectively. Growth performance was monitored over a 25-day feeding period, and fecal samples were collected for subsequent metagenomic sequencing and metabolomic analysis. Piglets supplemented with EU, EU+ME, and EU+HPE exhibited significantly improved growth performance, compared to the control group. Metagenomic analysis revealed significant alterations in gut microbiota composition, with increased beneficial bacterial classes and suppression of Prevotella spp. Metabolomic profiling demonstrated distinct metabolic alterations among the treatment groups, with pathway impact analysis highlighting enhanced protein synthesis and energy metabolism. Furthermore, EU supplementation did not affect porcine epidemic diarrhea virus activity in vitro but reduced LPS-induced intestinal inflammation. These findings suggest that EU could be a promising natural additive for improving piglet health and growth, with potential implications for managing post-weaning challenges in swine production.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503753"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of the serum progesterone strip test as a clinical tool for assessing reproductive status in replacement gilts. 使用血清孕酮试纸作为评估替代后备后备母猪生殖状况的临床工具。

IF 2.5 2区农林科学

Theriogenology Pub Date : 2025-12-01 Epub Date: 2025-08-06 DOI: 10.1016/j.theriogenology.2025.117627

Preechaphon Taechamaeteekul, Umaporn Pimpitak, Junpen Suwimonteerabutr, Wanwisa Poonlapdecha, Nutthiwut Khun-Arwut, Kittinan Komolpis, Sirirat Rengpipat, Kridtasak Sang-Gassanee, Hongyao Lin, Miquel Collell, Robert V Knox, Padet Tummaruk

{"title":"The use of the serum progesterone strip test as a clinical tool for assessing reproductive status in replacement gilts.","authors":"Preechaphon Taechamaeteekul, Umaporn Pimpitak, Junpen Suwimonteerabutr, Wanwisa Poonlapdecha, Nutthiwut Khun-Arwut, Kittinan Komolpis, Sirirat Rengpipat, Kridtasak Sang-Gassanee, Hongyao Lin, Miquel Collell, Robert V Knox, Padet Tummaruk","doi":"10.1016/j.theriogenology.2025.117627","DOIUrl":"10.1016/j.theriogenology.2025.117627","url":null,"abstract":"Identifying the reproductive status of replacement gilts at the appropriate age can improve mating efficiency and reduce non-productive days. This study aimed to evaluate the reproductive status of gilts and assess the accuracy of a serum progesterone strip test. A total of 112 Landrace × Yorkshire gilts, aged 196-238 days, were enrolled. Estrus detection was performed daily from 189 days of age using the back-pressure test with fence-line boar exposure. Blood samples were collected from all gilts, and serum progesterone levels were determined using both ELISA and a progesterone lateral flow assays (LFAs) strip test. Puberty was defined by an ELISA-based serum progesterone concentration ≥5.0 ng/ml. The LFAs employed a competitive assay using a monoclonal antibody against progesterone. A single test line indicated a 'positive' result (pubertal), while two lines indicated 'negative' (prepubertal). To perform the test, 2-3 drops of serum were applied to the strip, with results read after 15 min. Based on ELISA, 51.8 % of gilts were pubertal and 48.2 % were prepubertal. Pubertal gilts had significantly higher serum progesterone levels than prepubertal gilts (30.8 ± 11.4 vs. 0.8 ± 0.8 ng/ml; P < 0.001). The LFAs correctly identified 98.2 % of pubertal gilts and 94.4 % of prepubertal gilts, yielding a sensitivity of 98.2 % and specificity of 94.4 %. In conclusion, the progesterone strip test demonstrated high accuracy and shows strong potential for on-farm use in identifying pubertal gilts based on serum progesterone concentration.","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"248 ","pages":"117627"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of ineffectiveness of insulin-like growth factor 1 (IGF-1) on in vitro production of bovine embryos: systematic review and meta-analysis. 胰岛素样生长因子1 （IGF-1）对牛胚胎体外生产无效的证据：系统回顾和荟萃分析。

IF 2.5 2区农林科学

Theriogenology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI: 10.1016/j.theriogenology.2025.117626

Giovana de Santi Phelippe Nunes, Alan Brunholi Giroto, Sarah Gomes Nunes, Edson Assunção Mareco, Beatriz Díaz-Fabregat, Anthony César de Souza Castilho

{"title":"Evidence of ineffectiveness of insulin-like growth factor 1 (IGF-1) on in vitro production of bovine embryos: systematic review and meta-analysis.","authors":"Giovana de Santi Phelippe Nunes, Alan Brunholi Giroto, Sarah Gomes Nunes, Edson Assunção Mareco, Beatriz Díaz-Fabregat, Anthony César de Souza Castilho","doi":"10.1016/j.theriogenology.2025.117626","DOIUrl":"10.1016/j.theriogenology.2025.117626","url":null,"abstract":"Unsatisfactory results of in vitro maturation (IVM) impair the development, survival and implantation of the embryo. Insulin-like growth factor 1 (IGF-1) is widely used in IVM, but its efficacy is controversial and consistent evidence is lacking. To investigate the efficacy of IGF-1 in IVM, we conducted a systematic review and meta-analysis on bovine oocytes competence and subsequent embryonic development. We structured the research question according to the PICO model: \"Does IGF-1 enhance in vitro embryo production in cattle during maturation?\" We consulted scientific databases such as PubMed/MEDLINE, Embase and Scopus. The assessment of the methodological quality of the selected articles, the risk of bias, was performed with SYRCLE® by two reviewers. Data analysis was performed with R software, the \"META\" package for meta-analysis, using the metabin function to assess data proportions and estimate random effects, and the forest function for graphical representation. Of the 43 articles evaluated, eight were included in the statistical analysis. The results of the meta-analysis showed no significant effect of IGF-1 on cleavage rate (OR = 1.28 [0.96; 1.71]; p > 0.05) and on blastocyst yield (OR = 0.88 [0.64; 1.22]; p > 0.05). In conclusion, although IGF-1 is a key protein for cell differentiation, its addition during IVM does not promote blastocyst development. Therefore, we propose novel strategies to increase the bioavailability of IGF-1 during IVM in cattle to potentially improve embryonic outcomes.","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"248 ","pages":"117626"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High pathogenicity avian influenza H5N1 clade 2.3.4.4b natural infection in captive Humboldt penguins (Spheniscus humboldti). 洪堡企鹅自然感染高致病性H5N1进化枝2.3.4.4b

IF 2.2 2区农林科学

Avian Pathology Pub Date : 2025-12-01 Epub Date: 2025-07-16 DOI: 10.1080/03079457.2025.2513338

Audra-Lynne D Schlachter, Natalia Furman, Alexander M P Byrne, Scott M Reid, Sarah Jayne Smith, Daniel Maskell, Benjamin C Mollett, Jacob Peers-Dent, Marco Falchieri, Alex Schock, Ashley C Banyard, Ian H Brown, Alex Núñez

引用次数: 0

Perioperative drug management in non-critical companion animals: a retrospective study at a Spanish veterinary teaching hospital (2018-2022). 非关键伴侣动物围手术期药物管理：西班牙兽医教学医院回顾性研究（2018-2022）

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-06-06 DOI: 10.1080/01652176.2025.2513965

Beatriz Romero, Julen Susperregui, Ana M Sahagún, José R Altónaga, Cristina López, Raúl de la Puente, José M Rodríguez, Milena Vázquez, Raquel Díez

{"title":"Perioperative drug management in non-critical companion animals: a retrospective study at a Spanish veterinary teaching hospital (2018-2022).","authors":"Beatriz Romero, Julen Susperregui, Ana M Sahagún, José R Altónaga, Cristina López, Raúl de la Puente, José M Rodríguez, Milena Vázquez, Raquel Díez","doi":"10.1080/01652176.2025.2513965","DOIUrl":"10.1080/01652176.2025.2513965","url":null,"abstract":"There is little information in veterinary literature on the perioperative pharmacological management of small animal patients, despite the existence of common protocols and the importance of properly managing this period to reduce anaesthesia-related detrimental effects. This study aimed to analyse the current use of perioperative drugs in companion animals treated at the Veterinary Teaching Hospital of the University of León (HVULE) in Spain over a period of 5 years (2018-2022), describe the prescription patterns of these medicines, and identify the main variables associated with their prescription to explore possible strategies to promote their appropriateness. A total of 3438 cases were included in this study. The animals that most frequently underwent surgery were dogs (58.2%), females (57.0%), and adults (73.0%). The primary procedures performed were reproductive (56.6%) and traumatological (19.8%) surgeries. Regarding pharmacological treatments, more than half (62.3%) belonged to the ATCvet classification QN group (nervous system), and the most common compounds were isoflurane (13.5%), methadone (13.5%), and propofol (12.7%). Amoxicillin/clavulanic acid (3.7%) and marbofloxacin (2.8%) (categories C and B in the European Medicines Agency categorisation, respectively) were the most prescribed antibiotics. These findings provide detailed data to help veterinary policymakers improve drug use during surgical procedures.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of immune receptor knockout chickens via direct in vivo transfection of primordial germ cells. 通过直接在体内转染原始生殖细胞生产免疫受体敲除鸡。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-26 DOI: 10.1080/10495398.2025.2523027

Kristie Jenkins, Daniel Layton, Tamara Gough, Terri O'Neil, Luis Malaver Otega, Ketan Mishra, Kerri Bruce, Kirsten Morris, Terry Wise, Arjun Challagulla, Tim Doran, Andrew Bean

{"title":"Production of immune receptor knockout chickens via direct in vivo transfection of primordial germ cells.","authors":"Kristie Jenkins, Daniel Layton, Tamara Gough, Terri O'Neil, Luis Malaver Otega, Ketan Mishra, Kerri Bruce, Kirsten Morris, Terry Wise, Arjun Challagulla, Tim Doran, Andrew Bean","doi":"10.1080/10495398.2025.2523027","DOIUrl":"https://doi.org/10.1080/10495398.2025.2523027","url":null,"abstract":"The advancement of genetic engineering in chickens has enabled significant advancement in developmental biology, bioreactors, and disease resilience. The development of CRISPR/Cas9 genome engineering technology has further expanded the potential applications of genetic engineering in poultry. In this study we aimed to evaluate the efficacy of a direct in vivo transfection method, previously demonstrated to produce transgenic chickens, in generating gene knockout (KO) chickens. Specifically, we targeted the Interferon-α/β Receptor 1 (IFNAR1) and Interleukin 1 receptor, type I (IL1R1), both critical pathways in the inflammatory and antiviral responses. We designed guide RNAs targeting the genes and validated their efficiency in vivo via microinjection into the developing embryos. PCR analysis confirmed the presence of gene deletions in chimeric roosters, which were subsequently bred to produce G1 germline heterozygote KO offspring. Homozygous KO chickens were generated and subjected to phenotypic and functional analyses. Our results demonstrated successful generation of functional knockouts of both IFNAR1 and IL1R1 using a direct in vivo transfection. Overall, this study demonstrates that direct in vivo transfection provides a robust and predictable method for generating KO chickens, facilitating further research into avian immune responses and the development of antiviral strategies.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2523027"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples. 长期血清保存是否适合研究？时间和温度对犬血清抗利什曼原虫抗体测定的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-07-15 DOI: 10.1080/01652176.2025.2532396

Diana Marteles, María Eugenia Lebrero, Sergio Villanueva-Saz, Clara Esteban Sanz, Víctor Martín, Antonio Fernández, Pablo Quilez, Maite Verde, Patricia Galan-Malo, M Dolores Pérez

{"title":"Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples.","authors":"Diana Marteles, María Eugenia Lebrero, Sergio Villanueva-Saz, Clara Esteban Sanz, Víctor Martín, Antonio Fernández, Pablo Quilez, Maite Verde, Patricia Galan-Malo, M Dolores Pérez","doi":"10.1080/01652176.2025.2532396","DOIUrl":"10.1080/01652176.2025.2532396","url":null,"abstract":"The stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (-20 °C and -80 °C) over 2.5 years. Fifty-six serum samples were classified based on antibody concentration into low, medium, and high positive groups using an in-house enzyme-linked immunosorbent assay. Each sample was divided into aliquots and analyzed after different storage times (6 months, 1 year, 1.5, and 2.5 years). No statistically significant differences were observed in IgG concentrations across storage durations or between storage temperatures. Median antibody levels remained consistent, with minor variations attributed to assay-related variability. Correlation analyses showed strong agreement between initial and final measurements (R2 = 0.859 at -20 °C, R2 = 0.957 at -80 °C). The study underscores the suitability of -20 °C and -80 °C storage for preserving anti-Leishmania antibodies, providing valuable insights for serological diagnostics and research in veterinary science. Proper sample handling and aliquoting are recommended to maintain antibody integrity in routine diagnostics and long-term studies.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"11-20"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

农林科学最新文献