BioControlPub Date : 2026-01-01Epub Date: 2026-07-06DOI: 10.1007/s10526-026-10408-7
Chulantha P Diyes, Michelle Reid, Priyanka Mittapelly, Jennifer Holowachuk, Julian R Dupuis, Héctor A Cárcamo, Boyd A Mori
{"title":"Development of a multiplex PCR assay to identify two key alfalfa weevil (<i>Hypera postica</i>) parasitoids, <i>Bathyplectes curculionis</i> and <i>Oomyzus incertus</i>, in western North America.","authors":"Chulantha P Diyes, Michelle Reid, Priyanka Mittapelly, Jennifer Holowachuk, Julian R Dupuis, Héctor A Cárcamo, Boyd A Mori","doi":"10.1007/s10526-026-10408-7","DOIUrl":"https://doi.org/10.1007/s10526-026-10408-7","url":null,"abstract":"<p><p>The alfalfa weevil, <i>Hypera postica</i> Gyllenhal (Coleoptera: Curculionidae), is a major pest of alfalfa hay and seed production in North America. While chemical control methods can be effective, insecticide resistance has become a concern, and they can have negative impacts on non-target species including natural enemies and pollinators. Two European parasitoids, <i>Bathyplectes curculionis</i> Thomson (Hymenoptera: Ichneumonidae) and <i>Oomyzus incertus</i> Ratzeburg (Hymenoptera: Eulophidae), offer a potential alternative biological control strategy, but their prevalence in western Canada remains poorly understood. Traditional detection methods by live larval rearing, can have limited accuracy, particularly for parasitoid eggs and early instars. Here, we developed a novel multiplex PCR assay targeting the 5.8S ribosomal rDNA and ITS2 regions to detect <i>B. curculionis</i> and <i>O. incertus</i> within <i>H. postica</i> larvae. In 2020 and 2021, we assessed the parasitism rate of <i>H. postica</i> larvae from southern Alberta using both conventional live rearing (2021 only) and the multiplex PCR assay (2020-2021). Parasitism prevalence was significantly higher in 2021 (55.7%) compared to 2020 (6.9%), with multiparasitism changing little (10% to 11%). Live rearing predominantly detected <i>B. curculionis</i>, while <i>O. incertus</i> was nearly undetectable, whereas multiplex PCR revealed higher and more balanced parasitism prevalence for both parasitoid species. As susceptibility to parasitoids can differ between <i>H. postica</i> strains, we surveyed <i>H. postica</i> adults in Manitoba, Saskatchewan, and Alberta, and determined all were the western strain. These findings indicate that live rearing underestimates parasitism, confirm the dominance of the western strain in the Canadian prairies, and highlight the importance of molecular tools for host-parasitoid surveillance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at https://doi.org/10.1007/s10526-026-10408-7.</p>","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"71 5","pages":"639-650"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2025-01-01Epub Date: 2024-11-12DOI: 10.1007/s10526-024-10287-w
Jailma Rodrigues Dos Santos, Tamara Akemi Takahashi, Gabriel Rodrigues Palma, Rafael de Andrade Moral, José Roberto Postali Parra
{"title":"Parasitism by <i>Aleiodes ceres</i> Shimbori, 2023 (Hymenoptera: Braconidae) of three species of <i>Spodoptera</i> Guenée, 1852: effects of preferential instar and host diet.","authors":"Jailma Rodrigues Dos Santos, Tamara Akemi Takahashi, Gabriel Rodrigues Palma, Rafael de Andrade Moral, José Roberto Postali Parra","doi":"10.1007/s10526-024-10287-w","DOIUrl":"https://doi.org/10.1007/s10526-024-10287-w","url":null,"abstract":"<p><p>A recently discovered koinobiont parasitoid species, <i>Aleiodes ceres</i> Shimbori, 2023, proved to be a promising biological control agent for larvae of <i>Spodoptera cosmioides</i> (Walker, 1858), <i>S. eridania</i> (Stoll, 1782), and <i>S. frugiperda</i> (J.E. Smith, 1797), all well-known pests that cause critical damage to soybean and corn crops. This study investigated the potential of parasitism by <i>A. ceres</i> in these three species, including different instars (1st, 2nd, 3rd, and 4th instar) and diets (artificial and natural). Parasitism was highest when it occurs in the second instar for all three host species. The egg-to-pupa period of <i>A. ceres</i> was shorter in the second and third instars in relation to the first instar. Parasitism was substantially enhanced by the use of natural diets (soybean and corn leaves) compared with the artificial diet in all three species of the <i>Spodoptera</i> complex, suggesting a possible allelochemical interaction. Based on the results from the three host species, the potential for control of <i>Spodoptera</i> spp. by <i>A. ceres</i> was evident.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10526-024-10287-w.</p>","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"70 1","pages":"45-55"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11772551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2025-01-01Epub Date: 2025-08-05DOI: 10.1007/s10526-025-10338-w
David J Ensing, Tyler D Nelson, Chandra E Moffat, Lauryn Joslin, Lucas Eckert, Marlene M Kraml, Christopher G Eckert
{"title":"Together again: the invasive mustard <i>Hesperis matronalis</i> suffers devastating seed predation by a recently adventive specialist weevil.","authors":"David J Ensing, Tyler D Nelson, Chandra E Moffat, Lauryn Joslin, Lucas Eckert, Marlene M Kraml, Christopher G Eckert","doi":"10.1007/s10526-025-10338-w","DOIUrl":"10.1007/s10526-025-10338-w","url":null,"abstract":"<p><p>The enemy release hypothesis underpins classical (or importation) biocontrol as a management technique for invasive species. Classical biocontrol has had resounding success when prospective control agents have been subject to appropriate screening before release. Occasionally, however, natural enemies have been reunited with their hosts accidentally. Such adventive agents may provide effective control but have also avoided the careful screening characteristic of modern importation biocontrol programmes. We were studying the invasive mustard, <i>Hesperis matronalis</i> L. (Dame's rocket; Brassicaceae: Hesperidae), when we discovered rampant seed predation by an unknown seed predator. Using DNA barcoding, we identified this seed predator as <i>Ceutorhynchus inaffectatus</i> Gyllenhal (Coleoptera: Curculionidae), a recently (2018) detected species in North America. Comparing potential and realised seed production, we found that seed predation by <i>C. inaffectatus</i> strongly reduces <i>H. matronalis</i> fecundity, and that this effect was not moderated by infection with turnip mosaic virus (TuMV), a commercially important pathogen hosted by <i>H. matronalis</i> and transmitted by polyphagous aphid species. <i>C. inaffectatus</i> is expected to be highly host-specific, and the absence of native Hesperidae species in North America suggests the potential for <i>C. inaffectatus</i> as a classical, but adventive, biocontrol agent of <i>H. matronalis</i>. We suggest population genetic research to identify the origin of <i>C. inaffectatus</i>, and host specificity testing before any intentional redistribution of this species for <i>H. matronalis</i> biocontrol. More generally, this system acts as a model for biocontrol prospects with adventive insect herbivore species.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10526-025-10338-w.</p>","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"70 6","pages":"835-847"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12634765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2024-01-10DOI: 10.1007/s10526-023-10234-1
K. Yoneya, Satsuki Nishimori, Eizi Yano, Kei Yamaguchi, Matsuri Tsumoto, R. Ozawa, J. Takabayashi, I. Kandori
{"title":"Olfactory responses of Nesidiocoris tenuis to uninfested or conspecific-infested banker plants and Thrips palmi-infested eggplants","authors":"K. Yoneya, Satsuki Nishimori, Eizi Yano, Kei Yamaguchi, Matsuri Tsumoto, R. Ozawa, J. Takabayashi, I. Kandori","doi":"10.1007/s10526-023-10234-1","DOIUrl":"https://doi.org/10.1007/s10526-023-10234-1","url":null,"abstract":"","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"40 7","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139441162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2023-12-30DOI: 10.1007/s10526-023-10233-2
M. Aquino, E. Sujii, Sérgio Eustáquio Noronha, Miguel Borges, M. C. Blassioli‐Moraes, R. Laumann
{"title":"Influence of landscape structure on stink bug and their adult parasitoid populations on soybean culture","authors":"M. Aquino, E. Sujii, Sérgio Eustáquio Noronha, Miguel Borges, M. C. Blassioli‐Moraes, R. Laumann","doi":"10.1007/s10526-023-10233-2","DOIUrl":"https://doi.org/10.1007/s10526-023-10233-2","url":null,"abstract":"","PeriodicalId":8878,"journal":{"name":"BioControl","volume":" 2","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139140434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2023-12-21DOI: 10.1007/s10526-023-10220-7
J. Srikanth, B. Singaravelu, N. Crickmore, P. Mahesh, C. Sankaranarayanan, G. S. Suresha, C. Appunu, R. Nirmala, M. Om Prakash, K. M. Athira
{"title":"Laboratory evaluation of a scarabid-specific isolate of Bacillus thuringiensis against white grub Holotrichia serrata","authors":"J. Srikanth, B. Singaravelu, N. Crickmore, P. Mahesh, C. Sankaranarayanan, G. S. Suresha, C. Appunu, R. Nirmala, M. Om Prakash, K. M. Athira","doi":"10.1007/s10526-023-10220-7","DOIUrl":"https://doi.org/10.1007/s10526-023-10220-7","url":null,"abstract":"","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"35 23","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138952708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2023-12-12DOI: 10.1007/s10526-023-10229-y
Melissa C. Smith, Paul Julian, Don DeAngelis, Bo Zhang
{"title":"Ecological benefits of integrative weed management of Melaleuca quinquenervia in Big Cypress National Preserve","authors":"Melissa C. Smith, Paul Julian, Don DeAngelis, Bo Zhang","doi":"10.1007/s10526-023-10229-y","DOIUrl":"https://doi.org/10.1007/s10526-023-10229-y","url":null,"abstract":"","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"38 18","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioControlPub Date : 2023-12-08DOI: 10.1007/s10526-023-10226-1
Linda C. Muskat, Britta Kais, Jürgen Gross, Jørgen Eilenberg, Anant V. Patel
{"title":"A biobased superabsorbent formulation for above-ground application of a new entomophthoralean fungus for biological psyllid pest control","authors":"Linda C. Muskat, Britta Kais, Jürgen Gross, Jørgen Eilenberg, Anant V. Patel","doi":"10.1007/s10526-023-10226-1","DOIUrl":"https://doi.org/10.1007/s10526-023-10226-1","url":null,"abstract":"","PeriodicalId":8878,"journal":{"name":"BioControl","volume":"4 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138586609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}