Entomological Research最新文献

Entomopathogenic Potential of Cuticle-Degrading Enzymes From Bacillus velezensis CE100 in the Biocontrol of Pine Aphid (Eulachnus thunbergii Wilson) velezensis CE100表皮降解酶对松蚜的生物防治潜力

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-04-08 DOI: 10.1111/1748-5967.70117

Henry B. Ajuna, Sang-Jae Won, Jae-Hyun Moon, Vantha Choub, Su-In Choi, Ju-Yeol Yun, Su-Yeon Lee, Young Sang Ahn

{"title":"Entomopathogenic Potential of Cuticle-Degrading Enzymes From Bacillus velezensis CE100 in the Biocontrol of Pine Aphid (Eulachnus thunbergii Wilson)","authors":"Henry B. Ajuna, Sang-Jae Won, Jae-Hyun Moon, Vantha Choub, Su-In Choi, Ju-Yeol Yun, Su-Yeon Lee, Young Sang Ahn","doi":"10.1111/1748-5967.70117","DOIUrl":"10.1111/1748-5967.70117","url":null,"abstract":"Pine aphids are notoriously destructive sap-sucking pests of pine trees worldwide. We investigated the biocontrol efficacy of Bacillus velezensis CE100 through cuticle-degrading enzymes, which degrade the cuticular structure of insect pests. During its growth, CE100 produced protease, chitinase, and lipase activities up to 17 units/mL, 83 units/mL, and 27.5 units/mL, respectively. Treating aphid nymphs with bacterial broth culture and bacterial crude enzyme fraction of CE100 caused several lethal effects under laboratory conditions with up to 92% and 93% mortality rates, respectively, 30 h post-treatment. The median lethal time (ET50) of bacterial broth culture and crude enzyme fraction against aphid nymphs was 10 h, 36 min, and 5 h, 16 min, respectively. The aphids from the treatment groups exhibited severe fissures and disintegration of the cuticle, exposing internal tissues, and causing substantial loss of setae. The degradation of the wax layer, chitin, and glycoprotein polymers by the cuticle-degrading enzymes caused a high rate of aphid mortality, and thus, CE100 could effectively control aphid population and should be further studied as a potential component of integrated pest management strategies in pine forests.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Stress-Induced Expression of Antimicrobial Peptides SlAttacins in Shelfordella lateralis 侧Shelfordella slattins抗菌肽的鉴定及应激诱导表达

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-04-07 DOI: 10.1111/1748-5967.70115

Tielong Xu, Rui Zhu, Luyao Liu, Hui Lyu, Cao Zhou

{"title":"Identification and Stress-Induced Expression of Antimicrobial Peptides SlAttacins in Shelfordella lateralis","authors":"Tielong Xu, Rui Zhu, Luyao Liu, Hui Lyu, Cao Zhou","doi":"10.1111/1748-5967.70115","DOIUrl":"10.1111/1748-5967.70115","url":null,"abstract":"<div>\u0000 \u0000 The cherry red cockroach (Shelfordella lateralis) has potential medical application, but its antimicrobial peptides have not yet been studied. Using transcriptome-based gene cloning, bioinformatic analyses, and RT-qPCR, the sequences, structural features, and expression patterns of SlAttacin genes were systematically characterized. In this study, two Attacin genes (SlAttacin1 and SlAttacin2) in S. lateralis were identified; their sequences and expression profiles were analyzed. Both SlAttacins contain a signal peptide and conserved domains, with predicted protein molecular weights of 12.88 and 24.02 kDa, respectively. In addition, SlAttacin1 was highly expressed in female adults, while SlAttacin2 exhibited high expression in fourth-instar nymphs. Both SlAttacin genes were predominantly expressed in the fat body of nymphs. Moreover, bacterial infection (Escherichia coli and Staphylococcus aureus) significantly induced the transcriptional levels of SlAttacin, and exposure to deltamethrin (1 × 10−5 mg/L) significantly enhanced the expression of both SlAttacin genes. This study provides experimental evidence and theoretical support for the functional analysis of antimicrobial peptides in S. lateralis and their potential applications in the development of novel antimicrobial agents.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Preference Assessment of New Mosquito Attractants for Physical Control of Disease-Vector Mosquitoes 新型诱蚊剂的研制及其对病媒蚊虫的物理防治效果评价

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-04-07 DOI: 10.1111/1748-5967.70112

Seonguk Son, Nattawut Sareein, Jassada Saingamsook, Dong Gun Kim

{"title":"Development and Preference Assessment of New Mosquito Attractants for Physical Control of Disease-Vector Mosquitoes","authors":"Seonguk Son, Nattawut Sareein, Jassada Saingamsook, Dong Gun Kim","doi":"10.1111/1748-5967.70112","DOIUrl":"10.1111/1748-5967.70112","url":null,"abstract":"<div>\u0000 \u0000 This study focused on developing new mosquito attractants and evaluating their efficacy to control disease-vector mosquitoes through field experiments. As vectors of diseases such as malaria, dengue, and Zika, mosquitoes pose significant global health risks that are exacerbated by climate change and insecticide resistance. To address these challenges, eco-friendly alternatives are needed. Three newly formulated attractants (Type 1, Type 2, and Type 3) were tested against the commercial attractants BG-Lure and BG-Mozzibait in field trials in Thailand and South Korea. BG-Lure exhibited the highest overall efficacy, whereas Type 3 demonstrated superior performance in South Korea, achieving the highest daily average capture rate under certain conditions. In Thailand, Type 1 showed attraction rates comparable with those of BG-Mozzibait. These findings highlight the variability in attractant efficacy depending on location and mosquito species, underscoring the importance of tailored solutions for effective mosquito control and sampling for other study purposes. These newly developed attractants present sustainable alternatives to chemical control methods and offer valuable insights for improving public health strategies.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overwintering Ticks as a Reservoir for Severe Fever With Thrombocytopenia Syndrome Virus in the Republic of Korea 韩国越冬蜱作为严重发热伴血小板减少综合征病毒的宿主

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-04-06 DOI: 10.1111/1748-5967.70114

Hyunyoung Yoon, Hee Il Lee, Seong Yoon Kim

{"title":"Overwintering Ticks as a Reservoir for Severe Fever With Thrombocytopenia Syndrome Virus in the Republic of Korea","authors":"Hyunyoung Yoon, Hee Il Lee, Seong Yoon Kim","doi":"10.1111/1748-5967.70114","DOIUrl":"10.1111/1748-5967.70114","url":null,"abstract":"<div>\u0000 \u0000 Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease caused by SFTS virus (SFTSV), with reported case fatality rates of 10%–30% in Asia. In the Republic of Korea, over 170 cases are reported annually, with Haemaphysalis longicornis (Ixodida: Ixodidae) as the major vector. This tick species can overwinter as nymphs; however, its role in maintaining SFTSV during the winter remains unclear. This study aimed to identify overwintering ticks in areas adjacent to a fatal SFTS case, determine their infection rate, and evaluate the phylogenetic relationships among detected viruses. Within the residential area surrounding the site where the fatal case was reported, six heterogeneous habitat types were selected, comprising a vegetable garden, an outdoor water faucet, a dog kennel, a backyard, scrubland, and a nearby hill. To detect overwintering ticks, surveys were conducted using flagging and dry ice-baited traps, and samples of decaying wood and the ground layer comprising leaf litter, the soil surface, and the topsoil layer were collected. Overwintering ticks were detected only in soil samples from the outdoor water faucet and the nearby hill sites. A total of 58 nymphs were collected, including 5 from leaf litter, 34 from the soil surface layer, and 19 from the topsoil layer. SFTSV was detected in three ticks collected from the outdoor water faucet, including two specimens from the soil surface layer and one from the topsoil layer, resulting in an infection rate of 5.2%. Phylogenetic analysis identified the detected viruses as genotype B-2, with 99.6%–100% nucleotide identity to sequences from the patient and local ticks collected during the active season. These findings suggest that overwintering ticks in soil environments may contribute to viral persistence and early-season transmission risk.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Isothermal Amplification Assays for the Identification of Spodoptera eridania and Spodoptera ornithogalli (Lepidoptera: Noctuidae) Using Mitochondrial Gene Sequences 利用线粒体基因序列鉴定斑点夜蛾和鸟蛾夜蛾等温扩增方法的建立

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-04-05 DOI: 10.1111/1748-5967.70113

Seung Hyun Lee, Jee-Young Pyo, Min Jee Kim, Jeong Sun Park, Iksoo Kim

{"title":"Development of Isothermal Amplification Assays for the Identification of Spodoptera eridania and Spodoptera ornithogalli (Lepidoptera: Noctuidae) Using Mitochondrial Gene Sequences","authors":"Seung Hyun Lee, Jee-Young Pyo, Min Jee Kim, Jeong Sun Park, Iksoo Kim","doi":"10.1111/1748-5967.70113","DOIUrl":"10.1111/1748-5967.70113","url":null,"abstract":"Although the southern armyworm (SAW), Spodoptera eridania, and the yellow-striped armyworm (YSAW), Spodoptera ornithogalli, have not yet been introduced to Korea, they are listed as regulated quarantine pests in Korea because of their potential to cause serious economic losses in diverse agricultural crops upon introduction. Therefore, a preemptive diagnostic method that enables rapid and accurate identification is necessary to prevent the establishment of these species. In this study, we developed loop-mediated isothermal amplification (LAMP) assays for in-field use. To develop these assays, we sequenced the complete mitochondrial genomes (mitogenomes) of the two target species, SAW and YSAW, along with that of Spodoptera praefica. By comparing the three mitogenome sequences with publicly available sequences of 11 lepidopteran species, we preferentially selected sequences suitable for designing SAW- and YSAW-specific primers for the LAMP assays from the mitochondrial cytochrome oxidase subunit I gene (COI). The assays consistently diagnosed SAW and YSAW from 13 nontarget species, including the reciprocal target species, five Spodoptera species, and seven other lepidopteran species, within 25 min of reaction time using crude DNA. These LAMP assays could serve as rapid and accurate in-field detection tools for SAW and YSAW.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct Morphological Traits of Korean Populations of Pryeria sinica Moore (Lepidoptera: Zygaenidae) Associated With Regional Variation 中国早熟禾居群形态特征与区域变异的关系(鳞翅目：鲜蝇科

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-04-05 DOI: 10.1111/1748-5967.70116

Ji Yun Yeo, Min-Woo Lee, Il-Kwon Park

{"title":"Distinct Morphological Traits of Korean Populations of Pryeria sinica Moore (Lepidoptera: Zygaenidae) Associated With Regional Variation","authors":"Ji Yun Yeo, Min-Woo Lee, Il-Kwon Park","doi":"10.1111/1748-5967.70116","DOIUrl":"10.1111/1748-5967.70116","url":null,"abstract":"Pryeria sinica Moore is a significant pest of Celastraceae and Pentaphylacaceae plants, causing extensive defoliation in East Asia and beyond. Although its morphological and biological characteristics have been studied in China and Japan, detailed research on the Korean population remains limited. This study investigated the morphological characteristics of Korean P. sinica populations, including stage-specific changes, sexual dimorphism, cocoons, and wings. The morphological analysis revealed that the Korean population shows distinct differences from other East Asian populations in the distribution of spiracles and wing venation. Notably, the Korean population exhibited spiracles from the first to eighth abdominal segments, whereas the Taiwanese population lacked spiracles on the first segment. Wing venation patterns differed, particularly in the bifurcation of cubital and anal veins, suggesting divergence between Chinese and Taiwanese populations compared to Korean and Japanese populations. The morphological differences identified in this study are interpreted as regional variation influenced by local environmental conditions. However, whether such variation reflects environmentally induced phenotypic plasticity or more fundamental differences indicative of potential speciation requires further clarification through molecular studies.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 4","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Detection of Wolbachia in Field-Collected Aedes aegypti Mosquitoes From Jeddah, Saudi Arabia” 更正“在沙特阿拉伯吉达现场采集的埃及伊蚊中检出沃尔巴克氏体”

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-03-19 DOI: 10.1111/1748-5967.70109

引用次数: 0

Morphological Abnormalities and Biological Effects in the Porto Alegre Strain of Rhipicephalus microplus 阿雷格里港小头猪株的形态异常及其生物学效应

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-03-13 DOI: 10.1111/1748-5967.70110

Arlex Rodríguez-Durán, Lucas Teixeira de Castro, David Driemeier, Jesús Alfredo Cortés-Vecino, Luís Fernando Parizi, Itabajara da Silva Vaz Junior

{"title":"Morphological Abnormalities and Biological Effects in the Porto Alegre Strain of Rhipicephalus microplus","authors":"Arlex Rodríguez-Durán, Lucas Teixeira de Castro, David Driemeier, Jesús Alfredo Cortés-Vecino, Luís Fernando Parizi, Itabajara da Silva Vaz Junior","doi":"10.1111/1748-5967.70110","DOIUrl":"https://doi.org/10.1111/1748-5967.70110","url":null,"abstract":"Rhipicephalus microplus is a widespread tick species causing significant economic losses and transmitting pathogens to cattle. The R. microplus populations utilized in tick research are frequently maintained under laboratory conditions for many years. Despite this, morphological abnormalities developed in laboratory-maintained strains remain underexplored. Here, we analyzed the Porto Alegre (POA) strain, maintained under controlled conditions for nearly four decades, assessing external and internal morphological abnormalities across generations and their impact on biological parameters. We identified a low frequency (~0.9%) of diverse external malformations, predominantly idiosomal asymmetries, accompanied by histological gut alterations indicative of cellular dysfunction. Malformed ticks exhibited significantly reduced body weight, oviposition, and fertility compared to normal individuals. These abnormalities were not heritable but persisted sporadically, suggesting multifactorial origins. Our findings indicate that morphological malformations impair feeding efficiency and reproductive capacity, potentially limiting the fitness of affected ticks. This study enhances understanding of morphological variation in R. microplus and its biological implications and will inform strain maintenance and tick biology research.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 3","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The COI-COII Intergenic Spacer for Discriminating Honeybee Subspecies: Capabilities and Constraints 蜜蜂亚种的COI-COII基因间间隔：能力与限制

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-03-12 DOI: 10.1111/1748-5967.70111

Sara AlAshaal, Hossam F. Abou-Shaara, Areej A. Al-Khalaf, Eslam Adly, Mahmoud Kamal, Mohamed Nasser

{"title":"The COI-COII Intergenic Spacer for Discriminating Honeybee Subspecies: Capabilities and Constraints","authors":"Sara AlAshaal, Hossam F. Abou-Shaara, Areej A. Al-Khalaf, Eslam Adly, Mahmoud Kamal, Mohamed Nasser","doi":"10.1111/1748-5967.70111","DOIUrl":"https://doi.org/10.1111/1748-5967.70111","url":null,"abstract":"<div>\u0000 \u0000 Accurate identification of honeybee (Apis mellifera) subspecies is essential for conservation and breeding programs. The mitochondrial COI-COII intergenic spacer has been proposed as a genetic marker for subspecies discrimination, but its reliability remains debated. We analyzed 41 COI-COII sequences from diverse subspecies and geographic origins using phylogenetic reconstruction, network analysis, and population genetic statistics. Our results show that although this marker successfully distinguishes some regional populations (e.g., Russian, French, and Maltese specimens), it exhibits limited resolution for closely related subspecies. We detected only 27 polymorphic sites and found identical sequences shared among geographically distant and taxonomically distinct groups. Designed primers showed variable specificity, with subspecies-specific amplification achieved for A. m. ruttneri but not for A. m. lamarckii or A. m. carnica. These findings confirm that the COI-COII region alone is insufficient for comprehensive subspecies identification and should be combined with nuclear markers or morphological data in taxonomic and conservation applications.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 3","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Selection of optimal grain media for the production of entomopathogenic fungi using multiple evaluation methods” 对“利用多种评价方法选择生产昆虫病原真菌的最佳颗粒培养基”的修正

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-02-22 DOI: 10.1111/1748-5967.70108

引用次数: 0