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Activity of Essential Oils From Pentanema indicum (L.) Y. Ling and Chromolaena odorata (L.) R.M.King & H. Rob Against Three Mosquito Species
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-02-02 DOI: 10.1111/1748-5967.70016
Pathalam Ganesan, Jeyaraj Selvakumaran, Savarimuthu Ignacimuthu, Badr Aldahmash, Ahmed Rady, Fares Ahmed Alzahrani, Mansour Almansour, Antony Stalin
{"title":"Activity of Essential Oils From Pentanema indicum (L.) Y. Ling and Chromolaena odorata (L.) R.M.King & H. Rob Against Three Mosquito Species","authors":"Pathalam Ganesan,&nbsp;Jeyaraj Selvakumaran,&nbsp;Savarimuthu Ignacimuthu,&nbsp;Badr Aldahmash,&nbsp;Ahmed Rady,&nbsp;Fares Ahmed Alzahrani,&nbsp;Mansour Almansour,&nbsp;Antony Stalin","doi":"10.1111/1748-5967.70016","DOIUrl":"https://doi.org/10.1111/1748-5967.70016","url":null,"abstract":"<div>\u0000 \u0000 <p>Mosquitoes act as biological carriers spreading numerous vector borne diseases globally. The present research aimed to study the effect of essential oils (EOs) from <i>Pentanema indicum</i> (L.) Y. Ling and <i>Chromolaena odorata</i> (L.) R.M. King &amp; H. Rob against <i>Aedes aegypti, Anopheles stephensi</i> and <i>Culex quinquefasciatus</i> mosquitoes. Concentrations of 3.125, 6.25, 12.5 25 and 50 ppm were tested for their mosquitocidal properties. The essential oil (EO) from <i>P. indicum</i> demonstrated promising ovicidal activity, achieving 100% efficacy against <i>Ae. aegypti</i> and <i>Cx. quinquefasciatus</i> and 76% efficacy against <i>An. stephensi</i> at a concentration of 50 ppm within 24 h. Similarly, <i>C. odorata</i> exhibited significant activity at higher concentrations, with ovicidal rates of 72% for <i>Ae. aegypti</i>, 79% for <i>An. stephensi</i> and 64% for <i>Cx. quinquefasciatus</i> at 300 ppm after 72 h. In terms of larvicidal activity, <i>P. indicum</i> showed potent toxicity with LC<sub>50</sub> values of 4.28 ppm for <i>Ae. aegypti</i>, 14.01 ppm for <i>An. stephensi</i> and 5.47 ppm for <i>Cx. quinquefasciatus</i>. Additionally, <i>C. odorata</i> exhibited considerable mosquito larvicidal activity at higher concentrations, with LC<sub>50</sub> values of 95.28 ppm for <i>Ae. aegypti</i>, 98.95 ppm for <i>An. stephensi</i> and 89.05 ppm for <i>Cx. quinquefasciatus</i> after 24 h. However, no significant pupicidal activity was observed for both EOs. GC–MS analysis identified β-Pinene (14.31%), D-Limonene (13.48%) and Caryophyllene (25.48%) as the main components in <i>P. indicum</i>. The EO from <i>C. odorata</i> contained α-Pinene (12.10%), Geijerene (11.62%) and Caryophyllene (10.64%) as the primary components; probably these compounds were responsible for the observed activities. Toxicity studies indicated that the EOs from both plants had no adverse effects on natural predators. The EOs were then formulated for field application and tested in a laboratory setting; they demonstrated promising mosquitocidal activity without harming natural predators. This study suggests that EOs from <i>P. indicum</i> and <i>C. odorata</i> offer an effective alternative to synthetic pesticides.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 2","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the larvicidal potential and phytochemical composition of Solanum incanum extracts: Insights from molecular docking and GC–MS analysis
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-02-02 DOI: 10.1111/1748-5967.70011
Nael Abutaha, Fahd A. Al-mekhlafi, Nawaf.D. Almoutiri, Mohamed A. Wadaan, Mohammed S. Al-Khalifa
{"title":"Exploring the larvicidal potential and phytochemical composition of Solanum incanum extracts: Insights from molecular docking and GC–MS analysis","authors":"Nael Abutaha,&nbsp;Fahd A. Al-mekhlafi,&nbsp;Nawaf.D. Almoutiri,&nbsp;Mohamed A. Wadaan,&nbsp;Mohammed S. Al-Khalifa","doi":"10.1111/1748-5967.70011","DOIUrl":"https://doi.org/10.1111/1748-5967.70011","url":null,"abstract":"<p>Despite continuous efforts to control mosquito populations and disease vectors, they remain prevalent globally and pose significant health risks. Botanicals have garnered increasing attention as alternatives to synthetic insecticides due to their broad-spectrum insecticidal properties, biodegradability, and environmental compatibility. This study aimed to evaluate the larvicidal efficacy of <i>Solanum incanum</i> Soxhlet extracts using various solvents. Additionally, we conducted a phytochemical analysis of the methanol extracts using gas chromatography–mass spectrometry (GC–MS) and performed molecular docking studies. The methanol extract of <i>S. incanum</i> demonstrated significant larvicidal activity, with LC<sub>50</sub> values of 266.75, 214.52, and 90.15 μg/mL at 24, 48, and 72 hours post-treatment, respectively. GC–MS analysis identified several compounds in the methanol extract, with trans-9-Octadecenoic acid, pentyl ester (17.99%) and propanoic acid, 2-(3-acetoxy-4,4,14-trimethylandrost-8-en-17-yl) (14.42%) being the most abundant. The methanol extract exhibited the highest phenolic content, with 1.44 mg GAE/g. The IC50 values for the DPPH assay were 1.69 mg/mL for the ethyl acetate extract and 6.7 mg/mL for the methanol extract. Furthermore, molecular docking supported the findings, with propanoic acid, 2-(3-acetoxy-4,4,14-trimethylandrost-8-en-17-yl) emerging as the top compound for all targets tested, including AChE (−9.4 kcal/mol), GABA-T (−8 kcal/mol), and ATPase (−7.9 kcal/mol). These results provide valuable insights into the potential application and development of bioinsecticides.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 2","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biodiversity of Metarhizium From Korea and Pathogenicity Screening of Two Unrecorded Species Metarhizium lepidiotae and Metarhizium robertsii
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-02-02 DOI: 10.1111/1748-5967.70017
Seulki Kim, InJi Heo, Tae Young Shin
{"title":"Biodiversity of Metarhizium From Korea and Pathogenicity Screening of Two Unrecorded Species Metarhizium lepidiotae and Metarhizium robertsii","authors":"Seulki Kim,&nbsp;InJi Heo,&nbsp;Tae Young Shin","doi":"10.1111/1748-5967.70017","DOIUrl":"https://doi.org/10.1111/1748-5967.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>This study analyzed the biodiversity of entomopathogenic fungi <i>Metarhizium</i> species isolated from Korean soil samples and insect cadavers. Morphological characteristics and molecular phylogenetic analyses were used to analyze Korean species diversity. Concatenated datasets of three genes, the translation elongation factor 1-a (TEF), DNA-directed RNA polymerase II (RPB1), and internal transcribed spacer (ITS), were used for phylogenetic analyses using the following three methods: maximum parsimony (MP), Bayesian inference (BI), and maximum likelihood (ML). In addition, the TEF gene was used for molecular identification. As a result, five species were identified: <i>Metarhizium pinghaense</i>, <i>Metarhizium robertsii</i>, <i>Metarhizium rileyi</i>, <i>Metarhizium pemphigi</i>, and <i>Metarhizium lepidiotae</i>. In particular, <i>M. robertsii</i> and <i>M. lepidiotae</i> were identified in Korea for the first time in this study. The TEF gene was useful for species identification of <i>Metarhizium</i> species especially <i>M. pinghaense</i> and <i>Metarhizium anisopliae</i>. Among these fungi, only <i>M. lepidiotae</i> showed high pathogenicity against cotton aphids (<i>Aphis gossypii</i>) and lesser mealworm (<i>Alphitobius diaperinus</i>).</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 2","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the genetic and morphological diversity of Pantala flavescens across different south Korean river basins
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-01-21 DOI: 10.1111/1748-5967.70012
Da Som Park, Dohee Lee, Woong-Bae Park, Ji-Eun Lee, Jun-Sung Kim, Si-Ae Park, Jeong Chan Moon, Jun-Kyu Park, Yuno Do
{"title":"Exploring the genetic and morphological diversity of Pantala flavescens across different south Korean river basins","authors":"Da Som Park,&nbsp;Dohee Lee,&nbsp;Woong-Bae Park,&nbsp;Ji-Eun Lee,&nbsp;Jun-Sung Kim,&nbsp;Si-Ae Park,&nbsp;Jeong Chan Moon,&nbsp;Jun-Kyu Park,&nbsp;Yuno Do","doi":"10.1111/1748-5967.70012","DOIUrl":"https://doi.org/10.1111/1748-5967.70012","url":null,"abstract":"<p><i>Pantala flavescens</i>, commonly referred to as the globe skimmer or wandering glider, are migratory dragonflies that are distributed around the world whose genetic diversities and population structures have not been defined in South Korea. Thus, we explored the genetic diversities, population structures, and morphological adaptations of <i>P. flavescens</i> along four main river basins in South Korea. In the present study, we carried out landmark morphometric analysis, microsatellite genotyping, and haplotype network analysis to gain insight into the morphological divergence, genetic diversity, and evolutionary dynamics of the <i>P. flavescens</i> populations from four different river basins. Microsatellite markers and mitochondrial DNA haplotypes revealed moderate genetic diversity and significant gene flow among populations, showing minimal geographic differentiation; hence, this points toward a capacity for extensive gene exchange in this species. Wing morphometrical analysis showed clear morphological differences among populations. However, the fact that there is a lack of correlation between morphological differences and geographic distance argues against pure local environmental pressures driving wing morphology. Based on the global genetic connectivity observed using the haplotype network, <i>P. flavescens</i> represents a model organism to investigate the evolutionary consequences of migration over broad geographic scales. These findings have given important insights into mechanisms maintaining genetic diversity and adaptability in <i>P. flavescens</i> and provided valuable guidance for the conservation of migratory species under rapidly changing environmental conditions.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143117852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of disease-vector mosquitoes (Diptera: Culicidae) in the urban environment of Buraydah, Saudi Arabia
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-01-15 DOI: 10.1111/1748-5967.70015
Hayat S. Al-Rashidi, Hanan S. Alyahya, Wafa M. Al-Otaibi, Fekri M. Shaher, Abdullah G. Alghamdi, Mohammad M. Aljameeli, Talha A. Al-Dubai, Jazem A. Mahyoub
{"title":"Diversity of disease-vector mosquitoes (Diptera: Culicidae) in the urban environment of Buraydah, Saudi Arabia","authors":"Hayat S. Al-Rashidi,&nbsp;Hanan S. Alyahya,&nbsp;Wafa M. Al-Otaibi,&nbsp;Fekri M. Shaher,&nbsp;Abdullah G. Alghamdi,&nbsp;Mohammad M. Aljameeli,&nbsp;Talha A. Al-Dubai,&nbsp;Jazem A. Mahyoub","doi":"10.1111/1748-5967.70015","DOIUrl":"https://doi.org/10.1111/1748-5967.70015","url":null,"abstract":"<p>Mosquitoes are one of the most significant vectors of diseases worldwide. They can transmit many viral and parasitic diseases to humans and animals. A survey was conducted in ten areas in Buraydah City to investigate the prevalence and distribution of mosquitoes in the area. The study involved the collection of adult mosquitoes using Blackhole light traps over a year, from January to December 2021. During this period, a total of 23,346 adult mosquitoes were collected and analyzed as part of the survey. The results showed the presence of several mosquito species, including <i>Aedes aegypti, Ae. caspius, Culex pipiens, Cx. quinquefasciatus, Cx. citizens</i>, and <i>Anopheles d'thali</i>. This mosquito survey revealed the dominance of <i>Cx</i>. mosquitoes, with 21,216 mosquitoes collected out of a total of 23,346, representing 91% of the total. In contrast, the <i>Aedes</i> genus was the second most prevalent species, with 1,860 mosquitoes collected, representing 8% of the total mosquito population. <i>Anopheles</i> mosquitoes were remarkably low, with only 270 individuals collected, representing less than 1% of the total mosquito population. The survey revealed that mosquitoes are present throughout the year, but their density varies due to monthly changes in temperature and humidity. Furthermore, the first documented sighting of the <i>Ae. aegypti</i> mosquito in the city was recorded. This finding represents a serious public health concern, as <i>Ae. aegypti</i> is a known vector of diseases, especially dengue fever. Therefore, there is an urgent need to enhance mosquito surveillance and control measures in Buraydah to mitigate the risks of mosquito-borne disease outbreaks.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to ‘Evaluation of silver nanoformulated plant extracts against larvae of the fall armyworm, Spodoptera frugiperda (J. E. Smith), under laboratory and field conditions’
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-01-14 DOI: 10.1111/1748-5967.70014
{"title":"Correction to ‘Evaluation of silver nanoformulated plant extracts against larvae of the fall armyworm, Spodoptera frugiperda (J. E. Smith), under laboratory and field conditions’","authors":"","doi":"10.1111/1748-5967.70014","DOIUrl":"https://doi.org/10.1111/1748-5967.70014","url":null,"abstract":"<p>\u0000 <span>Abbas, U</span>, <span>Majeed, MZ</span>, <span>Alkherb, WAH</span> <i>et al</i>. (<span>2024</span>) <span>Evaluation of silver nanoformulated plant extracts against larvae of the fall armyworm, <i>Spodoptera frugiperda</i> (J. E. Smith), under laboratory and field conditions</span>. <i>Entomological Research</i>, <span>54</span>;e70003. https://doi.org/10.1111/1748-5967.70003.</p><p>The reference ‘Kalleshwaraswamy CM, Asokan R, Swamy HM <i>et al</i>. (2018) First report of the fall armyworm, <i>S. frugiperda</i> (JE Smith) (Lepidoptera: Noctuidae), an alien invasive pest on maize in India. <i>Pest Management in Horticultural Ecosystems</i> <b>24</b>:23–29. https://doi.org/10.1007/s41190-018-0012-8’ was incorrect. This should have read: ‘Sharanabasappa SD, Kalleshwara Swamy CM, Asokan R <i>et al</i>. (2018). First report of the fall armyworm, <i>S. frugiperda</i> (JE Smith) (Lepidoptera: Noctuidae), an alien invasive pest on maize in India. <i>Pest Management in Horticultural Ecosystems</i> <b>24</b>:23–29.’</p><p>We apologize for this error.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Supplementary bioactivities of Curcuma longa extracts and parasitoid Anisopteromalus calandrae as a postharvest eco-friendly management tactic against maize weevil, Sitophilus zeamais (Motschulsky) (coleoptera: Curculionidae)
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-01-14 DOI: 10.1111/1748-5967.70013
Muhammad Mamoon-ur-Rashid,  Riaz-ud-Din, Muhammad Safdar Baloch, Hussan Ara Begum, Douglas Law, Mohamed Farouk Elsadek, Khalid S. Al-Numair, Daniel K.Y. Tan, Muhammad Yasin
{"title":"Supplementary bioactivities of Curcuma longa extracts and parasitoid Anisopteromalus calandrae as a postharvest eco-friendly management tactic against maize weevil, Sitophilus zeamais (Motschulsky) (coleoptera: Curculionidae)","authors":"Muhammad Mamoon-ur-Rashid,&nbsp; Riaz-ud-Din,&nbsp;Muhammad Safdar Baloch,&nbsp;Hussan Ara Begum,&nbsp;Douglas Law,&nbsp;Mohamed Farouk Elsadek,&nbsp;Khalid S. Al-Numair,&nbsp;Daniel K.Y. Tan,&nbsp;Muhammad Yasin","doi":"10.1111/1748-5967.70013","DOIUrl":"https://doi.org/10.1111/1748-5967.70013","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigates the bioactivities of <i>C. longa</i> extracts and parasitoid <i>A. calandrae</i> against the maize weevil, a significant pest in tropical and subtropical regions. The experiments were conducted in a completely randomized design with five replications and six concentrations of <i>C. longa</i> in an incubator at 27 ± 3°C with 65 ± 5% R.H. and a photoperiod of 12:12 h (L:D). This study found that concentrations of <i>C. longa</i>, except higher concentrations (2.5 and 3%), had no significant effect on the settlement of <i>A. calandrae</i> adults on treated maize grains. Corn kernels treated with <i>C. longa</i> aqueous extracts at 0.5, 1, 1.5, and 2% concentrations showed no significant effect on parasitoid development and no significant differences compared to the untreated control. No significant (P &gt; 0.05) differences were found when <i>A. calandrae</i> was offered <i>Sitophilus zeamais</i> for parasitization on maize grains treated with 0.5, 1, 1.5, 2, and 2.5% concentrations of <i>C. longa</i> aqueous extracts. Corn kernels treated with <i>C. longa</i> extracts at the evaluated concentrations reduced the populations of <i>S. zeamais</i> by 20.06, 26.87, 35.19, 41.36, 52.97 and 62.54% whereas; treating with <i>C. longa</i> aqueous extracts at 0.5, 1, 1.5, 2, 2.5 and 3% in combination with the release of five pairs of parasitoids per treatment reduced the population of <i>S. zeamais</i> by 64.24, 66.42, 72.71, 80.87, 86.30 and 90.64% respectively. The combination of <i>C. longa</i> extracts at appropriate concentrations and the release of <i>A. calandrae</i> can effectively manage maize weevil in grain storages as an alternative to chemical insecticides.</p></div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143114973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent innovations in Baculovirus-insect cell expression Systems for Therapeutic Protein Production
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2024-12-22 DOI: 10.1111/1748-5967.70008
Hyunjoo Hwang, Minkue Kim, Yerin Kim, Peter Hinterdorfer, Jeong Hwan Lee, Kisung Ko
{"title":"Recent innovations in Baculovirus-insect cell expression Systems for Therapeutic Protein Production","authors":"Hyunjoo Hwang,&nbsp;Minkue Kim,&nbsp;Yerin Kim,&nbsp;Peter Hinterdorfer,&nbsp;Jeong Hwan Lee,&nbsp;Kisung Ko","doi":"10.1111/1748-5967.70008","DOIUrl":"https://doi.org/10.1111/1748-5967.70008","url":null,"abstract":"<p>The production of recombinant glycoproteins is pivotal for numerous therapies targeting human diseases, forming a cornerstone of the medical industry. The baculovirus-insect cell system has emerged as a leading method for producing high-value therapeutic glycoproteins. This system has several advantages, including rapid cultivation of insect cells, high production yields, and relatively straightforward post-translational processing. Insect cells, being eukaryotic, have functional glycosylation pathways, offering a significant advantage over prokaryotic cells which lack these capabilities. These advantages are particularly promising for the expression and testing of post-translational processing in <i>de novo</i> proteins, which are newly developed and extensively researched through AI-based computational methods. However, challenges remain, particularly with expression levels and glycosylation modifications, which need to be addressed to optimize protein production. This review aims to provide a comprehensive overview of recent advancements in the baculovirus-insect cell expression system, highlighting progress in insect cell utilization, expression optimization, glycosylation modifications, and the production of therapeutic proteins.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 12","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143253081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibody development for the diagnosis of Oryctes rhinoceros nudivirus
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2024-12-19 DOI: 10.1111/1748-5967.70005
Hyun-Soo Kim, Eunsun Kim, Kyungbo Kim, June-Sun Yoon
{"title":"Antibody development for the diagnosis of Oryctes rhinoceros nudivirus","authors":"Hyun-Soo Kim,&nbsp;Eunsun Kim,&nbsp;Kyungbo Kim,&nbsp;June-Sun Yoon","doi":"10.1111/1748-5967.70005","DOIUrl":"https://doi.org/10.1111/1748-5967.70005","url":null,"abstract":"<p>The Korean rhinoceros beetle, <i>Trypoxylus dichotomus</i>, an edible insect in the Republic of Korea, is an important insect for industrial farming, with various uses ranging from medical purposes to pets. However, there have been difficulties in the production and management of this species over the past 10 years as a result of nudivirus. This virus belongs to the family Nudiviridae, and infection with this virus proves fatal for rhinoceros beetles. The goal of this work was to develop an antibody for fundamental research on virus diagnosis. The <i>ODV-E66</i> gene encoding an envelope protein was selected for antibody production. The peptide region was derived from <i>in silico</i> analysis considering the conserved motif region and secondary structure of the peptide. In addition, the final peptide immunogen was validated by analyzing the physicochemical properties of the peptide to increase the rate of antibody formation. The production of polyclonal antibodies was induced by injecting peptide immunogens into rabbits. Antibody activity was evaluated using the enzyme-linked immunosorbent assay (ELISA) method. As a result, we confirmed that antibody formation was possible with nudivirus mimotopes, and we also confirmed that the antigen–antibody reaction differs depending on the peptide. These results suggest a new perspective for diagnosing insect viruses and contribute to the broader knowledge of peptide immunogens.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 12","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143252835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Odorant binding protein TcOBPC02 contributes to phytochemical defense in the red flour beetle, Tribolium castaneum
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2024-12-19 DOI: 10.1111/1748-5967.70009
Shanshan Gao, Mengyi Guo, Weicheng Yu, Wenbo Miao, Haofei Ya, Dongfeng Liu, Ruimin Li, Kunpeng Zhang
{"title":"Odorant binding protein TcOBPC02 contributes to phytochemical defense in the red flour beetle, Tribolium castaneum","authors":"Shanshan Gao,&nbsp;Mengyi Guo,&nbsp;Weicheng Yu,&nbsp;Wenbo Miao,&nbsp;Haofei Ya,&nbsp;Dongfeng Liu,&nbsp;Ruimin Li,&nbsp;Kunpeng Zhang","doi":"10.1111/1748-5967.70009","DOIUrl":"https://doi.org/10.1111/1748-5967.70009","url":null,"abstract":"<p>The red flour beetle, <i>Tribolium castaneum</i>, is an agricultural and storage pest with a global distribution. Studies have shown that eucalyptol has strong contact toxicity against larvae of this beetle, whereas odorant binding proteins (OBPs) are known to contribute to larval defenses against this phytochemical toxin. However, the mechanisms underlying the protective effect of insect <i>OBP</i>s against eucalyptol remain unclear. Here, <i>TcOBPC02</i> from <i>T</i><i>. castaneum</i> was cloned and characterized. Gene expression profile analysis showed that <i>TcOBPC02</i> is highly expressed at early larval and early pupal stages. Additionally, tissue expression profiling revealed that, in the adult, <i>TcOBPC02</i> was most highly expressed in the head, followed by the epidermis, whereas in larvae, <i>TcOBPC02</i> was mainly expressed in hemolymph and the epidermis. These developmental stages and tissues that exhibit high <i>TcOBPC02</i> expression are closely related to the detoxification of heterologous substances. Furthermore, the mRNA level of <i>TcOBPC02</i> was significantly increased after exposure to eucalyptol, whereas <i>TcOBPC02</i>-targeted RNA interference increased the susceptibility of <i>T. castaneum</i> to eucalyptol, indicating that <i>TcOBPC02</i> participates in the tolerance of this beetle to eucalyptol. Additionally, recombinant <i>TcOBPC02</i> was expressed in <i>Escherichia coli</i> and isolated, enabling a straightforward fluorescence competition binding assay. In combination, these results have demonstrated that <i>TcOBPC02</i> is required for defenses against phytochemicals in <i>T. castaneum</i>. This study provides a theoretical basis for understanding the mechanisms underlying the degradation of exogenous toxicants in insects and adds to the repertoire of potential target genes for pest control.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 12","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143252836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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