Entomological Research最新文献

{"title":"Regional Occurrence and Integrative Characterization of Larval Parasitoids Associated With Spodoptera exigua in Korea","authors":"Hyeokchan Kwon,&nbsp;Minyoung Choi,&nbsp;Dong jun Park,&nbsp;Minseop Noh,&nbsp;Murtaza Khan,&nbsp;Juil Kim","doi":"10.1111/1748-5967.70144","DOIUrl":"https://doi.org/10.1111/1748-5967.70144","url":null,"abstract":"<div>\u0000 \u0000 <p>The beet armyworm, <i>Spodoptera exigua</i> (Hübner) (Lepidoptera: Noctuidae), has expanded its geographic occurrence in Korea and causes substantial damage to a wide range of crops. Accurate documentation and identification of its associated parasitoids are important for understanding parasitoid diversity and supporting future ecological studies. In this study, larvae of <i>S. exigua</i> were collected from nine agricultural regions in Korea and reared to recover emerging parasitoids. To address taxonomic difficulties arising from morphological similarity among parasitoids, we combined morphological examination with molecular characterization based on the mitochondrial cytochrome c oxidase subunit I (COI) gene. Three parasitoid genera, <i>Microplitis</i>, <i>Meteorus</i>, and <i>Campoletis</i>, were recovered from field-collected hosts. Genus-level assignments were based on diagnostic morphological characters and were further supported by BLASTn similarity searches against reference sequences available in the NCBI database. The COI dataset was further examined using four complementary molecular species delimitation approaches (ABGD, ASAP, bPTP, and GMYC) to evaluate barcode-based group structure. The recovered parasitoid assemblage showed regional variation, with <i>Microplitis</i> occurring most widely, whereas <i>Meteorus</i> and <i>Campoletis</i> showed more restricted occurrence. Our results provide baseline information on parasitoids associated with <i>S. exigua</i> in Korea and demonstrate the usefulness of combining morphology, DNA barcodes, and multiple species delimitation approaches for regional surveys of parasitoid assemblages. These findings establish a baseline faunistic, taxonomic, and molecular framework for future studies on the diversity, taxonomy, and ecology of parasitoids associated with <i>S. exigua</i> in Korean agroecosystems.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 9","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Cloning and Expression Profiling of a c-Jun N-Terminal Kinase (TmJNK) in Response to Microbial Challenge in Tenebrio molitor","authors":"Seo Jin Lee,&nbsp;Ho Am Jang,&nbsp;Jae Hui Kim,&nbsp;Sung Min Ku,&nbsp;Dong Woo Kang,&nbsp;So Yeon Choi,&nbsp;Sungsin Jo,&nbsp;Sang Mok Jung,&nbsp;Chul-Hyun Kim,&nbsp;Yong Seok Lee,&nbsp;Yeon Soo Han,&nbsp;Yong Hun Jo","doi":"10.1111/1748-5967.70143","DOIUrl":"https://doi.org/10.1111/1748-5967.70143","url":null,"abstract":"<p>Insects exclusively utilize their innate immunity to combat various types of microbial infections. As a part of conserved signaling pathways of innate immunity, the c-Jun N-terminal kinase (JNK) signaling pathway is engaged in the regulation of various stresses, development, and immunity. However, the roles of JNK signaling pathway in <i>Tenebrio molitor</i> remain unclear. Therefore, the objective of the present study was to clone, characterize, and analyze the expression pattern of <i>TmJNK</i> gene in <i>T. molitor</i>. The bioinformatics analyses showed that the protein encoded by the <i>TmJNK</i> gene consists of 390 amino acids and contains one conserved catalytic domain of serine/threonine kinase. The <i>TmJNK</i> gene was expressed throughout all developmental stages, with the highest expression levels in egg stage. Tissue-specific expression analyses showed that <i>TmJNK</i> was highly expressed in hemocytes from young larvae and adults and increased in the gut from late larvae stage. In order to investigate its expression in immune response, the expression pattern of <i>TmJNK</i> was analyzed after the infection with three types of microorganisms, namely, Gram-negative, Gram-positive bacteria, and fungi. The results demonstrated that the expression pattern of <i>TmJNK</i> was tissue-specific and time dependent and that the kinase was induced in the gut, integument, hemocytes, fat body, and Malpighian tubules at different time points. These data indicated that <i>TmJNK</i> is an important conserved signaling molecule that may be involved in stress signals and innate immunity in <i>T. molitor</i>.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 9","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Distribution and Environmental Factor Analysis of the Invasive Alien Species Spotted Lanternfly (Lycorma delicatula) in South Korea","authors":"Minjun Kim,&nbsp;Jongchul Park,&nbsp;Youngho Cho,&nbsp;Youngjun Park","doi":"10.1111/1748-5967.70142","DOIUrl":"https://doi.org/10.1111/1748-5967.70142","url":null,"abstract":"<p>The spotted lanternfly (<i>Lycorma delicatula</i>) is an invasive alien species that has rapidly expanded across South Korea since its population began to surge in 2006. This study analyzed the spatiotemporal distribution patterns and environmental drivers of the species following its nationwide expansion, utilizing national survey data from 2016 to 2024, which were classified into three consecutive periods, and applying Local Moran's I and generalized additive models (GAM). Local Moran's I analysis revealed a spatial transition where high-high clusters shifted approximately twice as far from major grape-growing areas (21.88–40.59 km) between the first period (2016–2018) and the subsequent periods (2019–2024). This shift suggests that intensive, agriculture-centered pest control may temporarily displace populations to the periphery. GAM results showed that the models for the second and third periods explained over 60% of the deviance, with climatic variables such as temperature and precipitation emerging as highly significant predictors. Furthermore, an interaction analysis between human population size and distance to major vineyards indicated that high occurrence probabilities were maintained in densely populated areas near vineyards. These findings suggest that after intensive control is followed by spatial dispersal, populations can re-establish and stabilize in new habitats mediated by climatic suitability and urban connectivity. Future management strategies should transition from localized control to a broad-scale response system that integrates climatic suitability with human-spatial networks.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 9","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refinement of Habitat-Based Taxonomic Inventories for Benthic Macroinvertebrates: A Focus on Inland Water Ecosystem Surveys","authors":"Yung Chul Jun,&nbsp;Dongsoo Kong,&nbsp;Myoung-Chul Kim,&nbsp;Soon Jik Kwon,&nbsp;Youngjun Park,&nbsp;Ah. Reum Kim,&nbsp;Nan Young Kim,&nbsp;Yong Lak Jeon","doi":"10.1111/1748-5967.70141","DOIUrl":"https://doi.org/10.1111/1748-5967.70141","url":null,"abstract":"<div>\u0000 \u0000 <p>Benthic macroinvertebrates are essential biological indicators in biological monitoring and ecological health assessments of aquatic ecosystems. However, species information for benthic macroinvertebrates listed in the National List of Species of Korea has not been systematically organized according to habitat type, potentially leading to inefficiencies and inconsistencies for ecological surveys. This study aimed to organize habitat information for benthic macroinvertebrate species and to construct a habitat-based species list applicable to inland water ecosystem surveys. A total of 29,330 benthic macroinvertebrate species listed in the National List of Species of Korea were examined, and habitat information for each species was compiled through literature review and ecological data. Species were classified into five habitat categories: freshwater, brackish, marine, terrestrial, and data-deficient. As a result, terrestrial species accounted for the largest proportion with 21,453 species (73.2%), followed by marine species with 3951 species (13.5%), freshwater species with 1625 species (5.5%), and brackish species with 711 species (2.4%). In addition, 1590 species (5.4%) were classified as data-deficient. The habitat-based species list developed in this study enables a systematic organization of taxa with a high probability of occurrence in aquatic ecosystem surveys, thereby improving survey efficiency and enhancing consistency in data interpretation. Furthermore, the use of a standardized species list can reduce discrepancies in taxonomic identification among researchers and provide a reliable basis for long-term monitoring, including temporal comparisons with past and future datasets as well as comparisons across different ecological survey programs.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 9","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-Specific Transcriptomic Changes Associated With Mating Status in Overwintering Bombus terrestris Queens","authors":"Kyu-Won Kwak,&nbsp;Heeji Kim,&nbsp;Minwoong Son,&nbsp;Donghee Lee,&nbsp;Su-Jin Lee,&nbsp;Bo-Sun Park,&nbsp;Su-Bae Kim,&nbsp;Sung-Kuk Kim,&nbsp;Kyeoung Yong Lee","doi":"10.1111/1748-5967.70140","DOIUrl":"https://doi.org/10.1111/1748-5967.70140","url":null,"abstract":"<div>\u0000 \u0000 <p>Mating status is a key determinant of reproductive physiology in annual bumble bee queens, but its tissue-specific molecular effects after overwintering remain unclear. We analyzed RNA-seq data from the brains and ovaries of mated <i>Bombus terrestris</i> queens (BtQ) and unmated (virgin) queens (Bv) using tissue-wise combined biological replicates (<i>n</i> = 2 per mating-status group). Differentially expressed genes were identified with edgeR, and functional patterns were evaluated by Gene Ontology and KEGG enrichment analyses. Global clustering separated samples primarily by tissue and secondarily by mating status, indicating that tissue identity was the dominant source of transcriptomic variation. In the brain, BtQ-upregulated genes were enriched for DNA biosynthetic process, DNA polymerase complex, spliceosome, and rRNA-processing-related categories, whereas Bv-upregulated genes were associated with extracellular region, serine-type endopeptidase inhibitor activity, membrane, transmembrane transport, and oxidoreductase activity. In the ovary, Bv-upregulated genes were enriched for nucleic acid binding, homologous recombination-mediated double-strand break repair, tRNA methylation, germ-line stem cell division, and transposable element silencing. By contrast, BtQ-upregulated ovarian genes were enriched for heme binding, DNA biosynthetic process, monooxygenase activity, plasma membrane, oxidoreductase activity, DNA polymerase complex, defense response to bacterium, extracellular region, and iron ion binding, together with additional lipid- and metabolism-related categories. Overall, mating status reshaped overwintering brain and ovary transcriptomes through distinct tissue-specific programs, with the ovary showing the clearest contrast between a maintenance-oriented state in unmated queens and a biosynthetic, redox, membrane-associated, immune, and metabolic state in mated queens.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host Species Identity Is Associated With Distinct Frass-Associated Bacterial Communities in Spodoptera exigua (Hübner) and Pyrausta panopealis (Walker) Reared on a Shared Diet","authors":"Hee Jin Lee,&nbsp;Byung-Ryun Kim,&nbsp;Jong-Tae Jung,&nbsp;Meeja Seo,&nbsp;Moon Nam,&nbsp;Chan Pil Park","doi":"10.1111/1748-5967.70137","DOIUrl":"https://doi.org/10.1111/1748-5967.70137","url":null,"abstract":"<div>\u0000 \u0000 <p>Whether insect gut-associated microbial communities are structured by host identity or diet remains unresolved. Using a common-garden design, we reared two lepidopteran pests of contrasting host breadth, the polyphagous <i>Spodoptera exigua</i> (Hübner) and the Lamiaceae-associated <i>Pyrausta panopealis</i> (Walker), exclusively on perilla (<i>Perilla frutescens</i> [L.] Britton var. <i>frutescens</i>), and characterized frass-associated bacterial communities by 16S rRNA metabarcoding of three pooled replicates per species (240 larvae). The two hosts showed apparent separation in community composition (PERMANOVA <i>R</i>² = 0.857), which did not reach conventional significance (exact-permutation <i>p</i> = 0.100, the smallest value attainable with three replicates per group). Alpha diversity did not differ significantly (Wilcoxon rank sum, all <i>p</i> ≥ 0.10), although <i>S. exigua</i> showed numerically greater richness (median observed ASVs: 55 vs. 25) and <i>P. panopealis</i> showed higher evenness. Because the two species differ in taxonomic family, body size, and life history beyond host breadth, these patterns reflect a host species–associated difference under a shared diet rather than general evidence that dietary breadth shapes microbiome architecture. To our knowledge, this is the first description of the bacterial community of <i>P. panopealis</i>, and shows that two co-occurring pests reared on an identical diet were numerically dominated by mutually near-exclusive genera (<i>Enterococcus</i>, 72.2%, in <i>S. exigua</i> vs. <i>Enterobacter</i>, 71.8%, in <i>P. panopealis</i>), with complete rank separation in five genera. Species-level names are descriptive because the V3–V4 region gives limited subgenus resolution. These patterns are consistent with host-associated filtering contributing to frass community assembly, a hypothesis warranting confirmation with larger samples and additional diet treatments.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Assembly of Coxsackievirus A16 Virus-Like Particles in Insect Cells via VP4-Deficient P1 Expression","authors":"Hyun-Soo Kim,&nbsp;Hyuk-Jin Moon,&nbsp;Ra-Mi Woo,&nbsp;Jae-Bang Choi,&nbsp;Beom-Ku Han,&nbsp;Soo Dong Woo","doi":"10.1111/1748-5967.70139","DOIUrl":"https://doi.org/10.1111/1748-5967.70139","url":null,"abstract":"<p>Coxsackievirus A16 (CVA16) is a major causative agent of hand, foot, and mouth disease (HFMD), yet efficient virus-like particle (VLP)–based vaccine development remains challenging due to enteroviral structural complexity and the cytotoxicity of the 3CD protease required for polyprotein processing. In this study, we established an optimized production strategy for CVA16 VLPs using the baculovirus expression vector system (BEVS) by balancing structural protein expression and proteolytic cleavage. To mitigate 3CD-mediated cytotoxicity, we applied an attenuation strategy using the modified <i>p10</i>-BS5 promoter, which reduced transcriptional strength by ~90% and delayed protease expression, thereby extending the production window. We further examined the impact of P1 structural protein conformation and expression levels on VLP assembly. Unlike Enterovirus 71 (EV71), which favors high-level expression of full-length P1, CVA16 assembly was highly sensitive to expression dynamics. Optimal VLP formation was achieved using a standard expression vector (pAcPol) combined with a VP4-deficient P1 construct (ΔVP4). Under optimized conditions (multiplicity of infection 1, 7 days postinfection), the rAcPol-CV-ΔVP4-BS5 construct yielded the highest level of assembled particles. Transmission electron microscopy confirmed uniform, spherical VLPs (30–35 nm) resembling native CVA16 virions. These findings demonstrate that precise control of transcriptional stoichiometry and structural configuration is critical for high-yield CVA16 VLP production, providing a robust platform for multivalent HFMD vaccine development.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Composition and Bio-Efficacy of Agro-Waste Plant Extracts and Their Potential as Bioinsecticides Against Culex pipiens Mosquitoes","authors":"Laila Ali M. Al-shuraym,&nbsp;Lamya Ahmed Alkeridis,&nbsp;Hend M. Alharbi,&nbsp;Abdelfattah Selim,&nbsp;Mohamed M. Baz,&nbsp;Esraa A. Elhawary","doi":"10.1111/1748-5967.70138","DOIUrl":"https://doi.org/10.1111/1748-5967.70138","url":null,"abstract":"<div>\u0000 \u0000 <p>This study assessed the larvicidal activity of methanol and hexane fruit peel extracts from <i>Hylocereus polyrhizus</i> (red dragon fruit), <i>Citrus sinensis</i> (sweet orange), <i>Punica granatum</i> (pomegranate), <i>Annona cherimola</i> (cherimoya), and <i>Physalis peruviana</i> (groundcherry) against third-instar larvae of <i>Culex pipiens</i>, following WHO bioassay guidelines. Larval mortality was recorded at 24 and 48 h, and LC₅₀, LC₉₀, and LC₉₅ values were calculated. Methanol extracts demonstrated greater larvicidal activity compared with hexane extracts. The most effective plants were <i>H. polyrhizus</i>, <i>C. sinensis</i>, and <i>P. granatum</i>. Based on 48-h LC₅₀ values, methanol extract toxicity followed the order: <i>H. polyrhizus</i> (157.73 ppm) &lt; <i>C. sinensis</i> (180.22 ppm) &lt; <i>P. granatum</i> (190.38 ppm) &lt; <i>A. cherimola</i> (249.98 ppm) &lt; <i>P. peruviana</i> (507.68 ppm). For hexane extracts, the ranking was <i>H. polyrhizus</i> (176.57 ppm) &lt; <i>C. sinensis</i> (220.36 ppm) &lt; <i>P. granatum</i> (252.63 ppm) &lt; <i>A. cherimola</i> (307.69 ppm) &lt; <i>P. peruviana</i> (651.06 ppm). GC/MS analysis of the five <i>n</i>-hexane extracts identified 57 compounds, mainly alkanes, alkenes, aldehydes, esters, ketones, triterpenoids, and fatty acid esters. In <i>Physalis</i>, fatty acid esters were predominant, followed by fatty acids and related compounds. UPLC/MS analysis of methanol extracts tentatively identified 16, 23, 21, 5, and 27 metabolites in <i>Annona</i>, <i>Citrus</i>, <i>Hylocereus</i>, <i>Punica</i>, and <i>Physalis</i>, respectively. Overall, methanol peel extracts, particularly from <i>H. polyrhizus</i>, <i>C. sinensis</i>, and <i>P. granatum</i>, exhibited strong larvicidal activity against <i>Cx. pipiens</i>, highlighting their potential as eco-friendly alternatives for mosquito control.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Biology and Mating Frequency in the Common Bed Bugs, Cimex lectularius, and Tropical Bed Bugs, Cimex hemipterus (Hemiptera: Cimicidae)","authors":"Pranita Sukha,&nbsp;Natthaset Khunhom,&nbsp;Dae-Yun Kim","doi":"10.1111/1748-5967.70136","DOIUrl":"https://doi.org/10.1111/1748-5967.70136","url":null,"abstract":"<div>\u0000 \u0000 <p>Traumatic insemination is a reproductive strategy in cimicids that enhances male success but imposes physiological costs on females. Despite the global resurgence of bed bugs, comparative studies between the common bed bug, <i>Cimex lectularius</i>, and the tropical bed bug, <i>C. hemipterus</i>, remain limited. We examined reproductive parameters and mating frequency effects in field-collected populations maintained under controlled laboratory conditions using artificial membrane feeding with human blood. Virgin females failed to oviposit or produced only sterile eggs, confirming the absence of parthenogenesis. Comparative assays confirmed <i>C. lectularius</i> had longer oviposition periods and higher fecundity than <i>C. hemipterus</i> (<i>p</i> &lt; 0.05), consistent with previous laboratory and field studies. Mating frequency assays revealed divergent survival costs: none- and single-pairing sustained survival with limited reproduction, repeated-pairing increased fecundity (<i>C. lectularius</i>: 14.8 ± 6.7; <i>C. hemipterus</i>: 5.5 ± 3.1 eggs) with moderated mortality in <i>C. hemipterus</i> (20% by the fourth cycle), and continuous-pairing caused lethal outcomes in <i>C. hemipterus</i> females (60% mortality by the third cycle, 100% by the fourth), whereas <i>C. lectularius</i> maintained oviposition with zero mortality. Natural mortality was excluded by virgin and single-pairing controls, confirming excess mortality was attributable to mating stress. These findings highlight species-specific reproductive strategies, with tropical bed bugs facing lethal mating pressures that may promote female dispersal and explain their rapid invasion into nontropical regions. Reproductive suppression approaches may be effective in reducing invasion risk of <i>C. hemipterus</i>.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil-Applied Silicon Does Not Affect the Performance of Aphis craccivora on Cowpea Genotypes","authors":"João Vitor Soares Morais,&nbsp;Daniel Marques Pacheco,&nbsp;Thayline Rodrigues de Oliveira,&nbsp;Adriele de Castro Ferreira,&nbsp;Maria da Conceição dos Santos Filha,&nbsp;Jerlane Sousa Oliveira,&nbsp;Jenilton Gomes da Cunha,&nbsp;Maria Carolina Farias e Silva,&nbsp;Alexandre Igor de Azevedo Pereira,&nbsp;Bruno Ettore Pavan,&nbsp;Luciana Barboza Silva","doi":"10.1111/1748-5967.70135","DOIUrl":"https://doi.org/10.1111/1748-5967.70135","url":null,"abstract":"<p>Cowpea (<i>Vigna unguiculata</i>) is an important crop in tropical regions, where infestations of the black aphid, <i>Aphis craccivora</i> Koch (Hemiptera: Aphididae), can significantly reduce productivity. Silicon (Si) fertilization has been proposed as a strategy to enhance plant resistance to herbivores, although its effectiveness varies among plant–insect systems, particularly those involving phloem-feeding insects. This study evaluated whether soil-applied silicon affects aphid performance and plant physiological responses in cowpea genotypes. Three greenhouse bioassays were conducted to assess genotype-related variation in aphid preference and reproduction, the effects of two silicon sources, rice husk-derived biochar and commercial silicon oxide, on aphid abundance and plant physiological traits, and temporal changes in plant susceptibility under aphid infestation. Genotype-dependent differences were detected in nymph production, whereas adult colonization did not differ among genotypes, indicating that host suitability was primarily expressed at the reproductive level. Silicon supplementation increased leaf Si accumulation, with significant differences between genotypes; however, it did not affect aphid abundance, reproductive output, plant damage severity, chlorophyll-related parameters, or temporal patterns of infestation. Notably, the Crioulo genotype accumulated significantly more silicon than BRS Novaera, yet this increase was not associated with reduced aphid performance. Overall, despite promoting silicon accumulation in plant tissues, soil-applied silicon did not influence aphid performance or plant physiological responses under the tested conditions. These findings indicate that successful silicon uptake does not necessarily translate into enhanced resistance against aphids in cowpea.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148616673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}