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Increased Production of Nonenveloped Virus-Like Particles in a Neutral pH-Adapted Insect Cell Line 中性ph适应昆虫细胞系非包膜病毒样颗粒产量的增加
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-27 DOI: 10.1111/1748-5967.70039
Jin Woong Im, Hyuk-Jin Moon, Soo Dong Woo
{"title":"Increased Production of Nonenveloped Virus-Like Particles in a Neutral pH-Adapted Insect Cell Line","authors":"Jin Woong Im,&nbsp;Hyuk-Jin Moon,&nbsp;Soo Dong Woo","doi":"10.1111/1748-5967.70039","DOIUrl":"https://doi.org/10.1111/1748-5967.70039","url":null,"abstract":"<div>\u0000 \u0000 <p>Virus-like particles (VLPs) are vaccine antigens that are actively being developed as vaccines because of their high immunogenicity and safety. Among the cell culture parameters for VLP production, pH can have a significant effect on VLP formation and stability. In this study, the pH of cultured insect cells (6.2) was adjusted to a neutral pH, and the effect on VLP production was evaluated. A neutral pH-adapted High Five insect cell line was established and named HF7. Compared with parent High Five cells, HF7 cells were approximately 2 μm larger and showed approximately sixfold greater viral replication. The production of nonenveloped VLPs in HF7 cells was evaluated using enterovirus 71 (EV-71), porcine parvovirus (PPV), porcine circovirus Type 2 (PCV2), and canine parvovirus (CPV) VLPs. As a result, increased production of all VLPs and VLP formation were confirmed. However, the increased production of VLPs is thought to be due to increased cell metabolism and virus replication rather than the formation or stability of VLPs. The neutral pH-adapted HF7 cell line established in this study can be utilized to increase the production of various recombinant proteins, including VLPs.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unrecognized Ant Megadiversity in the Australian Monsoonal Tropics: The Melophorus castaneus Heterick, Castalanelli & Shattuck Species Group (Hymenoptera: Formicidae) 澳大利亚季风热带地区未被认识的蚂蚁大多样性:Melophorus castaneus Heterick, Castalanelli & Shattuck种群(膜翅目:蚁科)
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-26 DOI: 10.1111/1748-5967.70037
Alan N. Andersen, François Brassard, Benjamin D. Hoffmann
{"title":"Unrecognized Ant Megadiversity in the Australian Monsoonal Tropics: The Melophorus castaneus Heterick, Castalanelli & Shattuck Species Group (Hymenoptera: Formicidae)","authors":"Alan N. Andersen,&nbsp;François Brassard,&nbsp;Benjamin D. Hoffmann","doi":"10.1111/1748-5967.70037","DOIUrl":"https://doi.org/10.1111/1748-5967.70037","url":null,"abstract":"<p>This paper continues the documentation of remarkable levels of undescribed ant diversity in the Australian monsoonal (seasonal) tropics. It considers the <i>Melophorus castaneus</i> Heterick, Castalanelli &amp; Shattuck species group, as considered here consisting of four described species: <i>M. castaneus</i>, <i>M. clypeatus</i> Heterick, Castalanelli &amp; Shattuck, <i>M. kuklos</i> Heterick, Castalanelli &amp; Shattuck, and <i>M. teretinotus</i> Heterick, Castalanelli &amp; Shattuck. Based on an integration of morphological, genetic (CO1), and distributional information, we recognize 24 species among 79 sequenced specimens from the group in monsoonal Australia, only one of which (<i>M. teretinotus</i>) is described. Our species delimitations are strongly supported by species inference from the Poisson tree processes model. We provide images of all our recognized species, along with images of four additional monsoonal species from the group that were not sequenced. Of the 28 monsoonal species in total that we document, 20 occur in the Top End of the Northern Territory, and none of these are known from elsewhere. Most locations within the Top End remain unsampled, and so many more species can therefore be expected to occur in it, and much more so elsewhere in monsoonal Australia. The total number of monsoonal species within the <i>M. castaneus</i> group is therefore very likely well over 50, only one of which is described. Our findings provide further evidence that the ant fauna of monsoonal Australia numbers several thousand species, a number far higher than estimated for any other of the world's regional ant faunas.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of DENV-Mediated Potential of Aedes albopictus (KDCA Strain) Through Comparative Experiments With Aedes aegypti 通过与埃及伊蚊的对比实验评价白纹伊蚊(KDCA株)denv介导的潜势
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-26 DOI: 10.1111/1748-5967.70030
Jie Eun Park, Sung-Jae Cha, Wei Huang, Min Kyu Sang, Jun Yang Jeong, Bharat Bhusan Patnaik, Se Won Kang, So Young Park, Hee Ju Hwang, Hang Chul Cho, Jong Min Chung, Jung Han Park, E. Hyun Shin, Hyunwoo Kim, Wook-Gyo Lee, Hee Il Lee, Lee Hyeong Woo, Yong Hun Jo, Yeon Soo Han, Marcelo Jacobs-Lorena, Yong Seok Lee
{"title":"Evaluation of DENV-Mediated Potential of Aedes albopictus (KDCA Strain) Through Comparative Experiments With Aedes aegypti","authors":"Jie Eun Park,&nbsp;Sung-Jae Cha,&nbsp;Wei Huang,&nbsp;Min Kyu Sang,&nbsp;Jun Yang Jeong,&nbsp;Bharat Bhusan Patnaik,&nbsp;Se Won Kang,&nbsp;So Young Park,&nbsp;Hee Ju Hwang,&nbsp;Hang Chul Cho,&nbsp;Jong Min Chung,&nbsp;Jung Han Park,&nbsp;E. Hyun Shin,&nbsp;Hyunwoo Kim,&nbsp;Wook-Gyo Lee,&nbsp;Hee Il Lee,&nbsp;Lee Hyeong Woo,&nbsp;Yong Hun Jo,&nbsp;Yeon Soo Han,&nbsp;Marcelo Jacobs-Lorena,&nbsp;Yong Seok Lee","doi":"10.1111/1748-5967.70030","DOIUrl":"https://doi.org/10.1111/1748-5967.70030","url":null,"abstract":"<p><i>Aedes albopictus</i> is an important vector of various arboviruses, including dengue virus (DENV). The vector capacity for DENV has been studied in various geographical regions, but information on Korean <i>Ae. albopictus</i> strains are limited. This study aimed to investigate the vector capacity of the Korean <i>Ae. albopictus</i> strain (Korea Disease Control and prevention Agency, KDCA) for DENV-2. Adult mosquitoes were orally infected with DENV-2 and dissected on days 3 and 7 post-infection. Viral RNA was extracted from midgut and carcass tissues and detected by RT-PCR. The results showed that the KDCA strain could acquire and transmit DENV-2, although with lower efficiency compared with <i>Ae. aegypti</i>. These findings highlight the potential risk of DENV transmission by <i>Ae. albopictus</i> strain (KDCA) and emphasize the need for effective vector control strategies.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Time-Lapse Imaging Identifies Key Indicators of Brown Planthopper Damage Progression in Rice Varieties 延时成像识别水稻品种褐飞虱危害进展的关键指标
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-24 DOI: 10.1111/1748-5967.70035
Nak Jung Choi, Kibon Ku, Sheikh Mansoor, Anh Tuan Le, Thanh Tuan Thai, E. M. B. M. Karunathilake, Jisoo Kim, Yong Suk Chung
{"title":"Time-Lapse Imaging Identifies Key Indicators of Brown Planthopper Damage Progression in Rice Varieties","authors":"Nak Jung Choi,&nbsp;Kibon Ku,&nbsp;Sheikh Mansoor,&nbsp;Anh Tuan Le,&nbsp;Thanh Tuan Thai,&nbsp;E. M. B. M. Karunathilake,&nbsp;Jisoo Kim,&nbsp;Yong Suk Chung","doi":"10.1111/1748-5967.70035","DOIUrl":"https://doi.org/10.1111/1748-5967.70035","url":null,"abstract":"<div>\u0000 \u0000 <p>Improving complex agronomic traits such as yield, plant height, and disease resistance in rice (<i>Oryza sativa</i> L.) is challenging due to their polygenic nature. Conventional breeding methods, often reliant on collective parameters, face limitations in efficiently identifying superior genotypes. Phenomics, however, presents a promising, targeted approach by focusing on individual phenotypic traits. This study employs time-lapse imaging to monitor dynamic phenotypic changes in rice plants infested with the brown planthopper (BPH) (<i>Nilaparvata lugens</i>). Utilizing the Phlizon 6000 W LED Grow Light, which provides far-red wavelengths, and image acquisition with 12 Raspberry Pi 4 Model B units and Raspberry Pi NoIR cameras fitted with blue filters for enhanced NDVI calculations, we captured detailed imagery of plant responses. Our study revealed that NDVI values in the stem region of control plants remained stable, while leaf region values showed an increase. For infested plants, NDVI fluctuations were observed at the lamina joint in the stem region, whereas leaf region values remained consistent. Importantly, damage progression was slower at the lamina joint in resistant rice varieties compared to susceptible ones, underscoring lamina joint discoloration as a valuable parameter for evaluating BPH resistance. This phenome-based precision breeding approach holds significant potential for accelerating the development of rice varieties with enhanced resistance to this pervasive pest, offering new avenues for improving crop resilience and yield.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Why Is Insecticide Resistance Rare in Malaria Vectors in Thailand? 为何泰国疟疾病媒很少对杀虫剂产生抗药性?
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-20 DOI: 10.1111/1748-5967.70032
Theeraphap Chareonviriyaphap, Daeyun Kim, John Aerol Nobleza, Suthat Lhaosudto, Chauwat Charoenwiriyapap
{"title":"Why Is Insecticide Resistance Rare in Malaria Vectors in Thailand?","authors":"Theeraphap Chareonviriyaphap,&nbsp;Daeyun Kim,&nbsp;John Aerol Nobleza,&nbsp;Suthat Lhaosudto,&nbsp;Chauwat Charoenwiriyapap","doi":"10.1111/1748-5967.70032","DOIUrl":"https://doi.org/10.1111/1748-5967.70032","url":null,"abstract":"<div>\u0000 \u0000 <p>Insecticide resistance in mosquito populations is an important area in the insecticide-disease control equation. Two major types of insecticide responses have been well recognized: physiological resistance and behavioral avoidance. Globally, the development of physiological resistance to insecticides among insect groups and disease vectors has been widely documented. In Thailand, physiological resistance has emerged across all major classes of synthetic insecticides where the majority are synthetic pyrethroids. Interestingly, the physiological resistance remains limited in <i>Anopheles</i> vectors despite the long-term use of synthetic compounds. This phenomenon suggests that behavioral responses may play a key role in disease prevention while simultaneously reducing the likelihood of insecticide resistance in mosquito vectors.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbiome Composition of Haemaphysalis flava in Korea and Diversity Analysis Based on Region, Developmental Stage, and Sex 韩国黄血蜱微生物组组成及地域、发育阶段和性别多样性分析
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-17 DOI: 10.1111/1748-5967.70031
Min Kyu Sang, Jie eun Park, Dae Kwon Song, Jun Yang Jeong, Chan-Eui Hong, Hyeonjun Shin, Hyeok Lee, Kyoung Won Lee, Hee Ju Hwang, Hyun woo Kim, Seong Yoon Kim, Wook-Gyo Lee, So Young Park, Se Won Kang, Jung Han Park, Bharat Bhusan Patnaik, Sung-Jae Cha, Sungsin Jo, Yong Hun Jo, Yeon Soo Han, Hee Il Lee, Yong Seok Lee
{"title":"Microbiome Composition of Haemaphysalis flava in Korea and Diversity Analysis Based on Region, Developmental Stage, and Sex","authors":"Min Kyu Sang,&nbsp;Jie eun Park,&nbsp;Dae Kwon Song,&nbsp;Jun Yang Jeong,&nbsp;Chan-Eui Hong,&nbsp;Hyeonjun Shin,&nbsp;Hyeok Lee,&nbsp;Kyoung Won Lee,&nbsp;Hee Ju Hwang,&nbsp;Hyun woo Kim,&nbsp;Seong Yoon Kim,&nbsp;Wook-Gyo Lee,&nbsp;So Young Park,&nbsp;Se Won Kang,&nbsp;Jung Han Park,&nbsp;Bharat Bhusan Patnaik,&nbsp;Sung-Jae Cha,&nbsp;Sungsin Jo,&nbsp;Yong Hun Jo,&nbsp;Yeon Soo Han,&nbsp;Hee Il Lee,&nbsp;Yong Seok Lee","doi":"10.1111/1748-5967.70031","DOIUrl":"https://doi.org/10.1111/1748-5967.70031","url":null,"abstract":"<p>Ticks are well-known vectors of various diseases and are widely distributed globally. In this study, we investigated the microbial community of <i>Haemaphysalis flava</i>, a common tick species in Korea, using 16S rRNA gene analysis. Alpha diversity analysis revealed that adult ticks had higher microbial richness and complexity than nymphs, and the highest diversity was observed in adult females. Significant regional differences were also found, with samples from Jinju and Boseong showing higher richness and samples from Jeju showing lower richness. Beta diversity analysis showed that microbial communities were clustered according to region and developmental stage, with unique clusters observed in Samcheok and Gokseong, which may be due to regional environmental factors. Proteobacteria dominated the microbial community in most samples, followed by Actinobacteriota and Firmicutes. At the genus level, <i>Coxiella</i> and <i>Pseudomonas</i> were dominant, and <i>Coxiella</i> may be associated with pathogen transmission. The abundance of other genera varied by region and developmental stage, reflecting complex host–microbe interactions. These findings highlight the influence of environmental, developmental, and biological factors on the <i>H. flava</i> microbiota, emphasizing the need for further research to understand the ecological role of the microbiota in pathogen transmission and to develop effective strategies for preventing tick-borne diseases.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wasp-Hive Candidate Site Search System Using a Small Drone 使用小型无人机的黄蜂蜂巢候选站点搜索系统
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-17 DOI: 10.1111/1748-5967.70034
Bosung Kim, Jeonghyeon Pak, Hyoung Il Son
{"title":"Wasp-Hive Candidate Site Search System Using a Small Drone","authors":"Bosung Kim,&nbsp;Jeonghyeon Pak,&nbsp;Hyoung Il Son","doi":"10.1111/1748-5967.70034","DOIUrl":"https://doi.org/10.1111/1748-5967.70034","url":null,"abstract":"<div>\u0000 \u0000 <p>Early detection of wasp hives is crucial for mitigating their impact on native species, preventing agricultural damage, and improving pest control strategies. Traditional detection methods rely on ground surveys and sensor-based tracking of individual insects, which are often labor-intensive, time-consuming, and prone to errors because of environmental constraints. The integration of artificial intelligence and drone-based imaging has the potential to revolutionize ecological monitoring by providing scalable, efficient, and noninvasive methods for detecting wasp hives. However, research on AI-assisted hive detection remains limited, with most studies focusing on large-scale wildlife monitoring rather than small-object localization. Therefore, we propose a system for searching the candidate site of a wasp hive using a small drone. In the proposed system, a small drone is equipped with a camera and takes aerial images of the error range. Subsequently, three-dimensional (3D) modeling is performed on the captured images using a 3D surveying toolkit, and deep learning–based hive detection is performed on the completed 3D model to extract the GPS information of the detected target.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Constant and Fluctuating Temperatures on Development and Fertility of Myzus persicae Sulzer (Hemiptera: Aphididae) 恒温和波动温度对桃蚜发育和育性的影响(半翅目:蚜科)
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-14 DOI: 10.1111/1748-5967.70033
Jeong Joon Ahn, Byung-Hyuk Kim, Jung-Eun Kim, Chun Hwan Kim
{"title":"Impact of Constant and Fluctuating Temperatures on Development and Fertility of Myzus persicae Sulzer (Hemiptera: Aphididae)","authors":"Jeong Joon Ahn,&nbsp;Byung-Hyuk Kim,&nbsp;Jung-Eun Kim,&nbsp;Chun Hwan Kim","doi":"10.1111/1748-5967.70033","DOIUrl":"https://doi.org/10.1111/1748-5967.70033","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluates the effects of constant and fluctuating temperatures on the development, survival, and fertility of <i>Myzus persicae</i>, with an emphasis on understanding how thermal variability shapes its life history traits. Moderate temperature fluctuations (10 ± 5°C, 15 ± 5°C, 20 ± 5°C, and 25 ± 5°C) significantly enhanced developmental rates and reproductive success compared to constant conditions, demonstrating increased metabolic efficiency and adaptability under variable thermal environments. In contrast, extreme fluctuations (30 ± 5°C) imposed physiological stress, leading to delayed development, reduced survival, and decreased reproductive output, indicating limited tolerance of <i>M. persicae</i> to high thermal variability. A temperature-dependent model revealed an optimal range around 25°C for developmental efficiency and reproductive performance. Fertility peaked at 20 ± 5°C but was markedly suppressed at 30 ± 5°C, underscoring the detrimental effects of extreme thermal fluctuations on population growth. These findings suggest that moderate thermal variability may promote population expansion of <i>M. persicae</i> under favorable conditions, whereas extreme fluctuations could act as natural constraints, limiting its dynamics in warmer climates. This study provides critical insights into the adaptability of <i>M. persicae</i> to thermal variability, offering a framework for predicting its responses to climate change. However, the observed physiological limitations under elevated temperatures highlight the need for further field-based studies that incorporate ecological complexity. Such research will be essential to improve predictions of <i>M. persicae</i> population dynamics and to develop informed pest management strategies in agroecosystems experiencing increasing temperature fluctuations.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Comprehensive Study of Insect Pupae in Forensic Entomology: X-Ray Imaging for Enhanced Post-Mortem Interval (PMI) Estimation 法医昆虫学中昆虫蛹的综合研究:x射线成像增强死后间隔(PMI)估计
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-13 DOI: 10.1111/1748-5967.70028
Woong-Bae Park, Jun-Kyu Park, Yuno Do
{"title":"A Comprehensive Study of Insect Pupae in Forensic Entomology: X-Ray Imaging for Enhanced Post-Mortem Interval (PMI) Estimation","authors":"Woong-Bae Park,&nbsp;Jun-Kyu Park,&nbsp;Yuno Do","doi":"10.1111/1748-5967.70028","DOIUrl":"https://doi.org/10.1111/1748-5967.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>Forensic entomology is a sub-discipline of entomology concerning insects and arthropods with their relationship to criminal investigations. Most important that comes out of it is the finding of an exact time of occurrence of a dead body by estimating the post-mortem interval (PMI). Our study focused on blow fly pupae, which are some of the earliest insects to colonize decomposing bodies. The pupae of the blow fly have the longest development period in the blow fly life history, but we have trouble viewing the inside of the pupae because of the puparium. It is, therefore, a developmental internal X-ray imaging stage carried out to see if such a division experiment could reveal a difference in growth based on temperatures in two different temperatures. All experiments were recorded accurately with an indoor Wi-Fi camera. Finally, the sampling individual in 20 °C and 30 °C shows a significant difference at the developmental time of 10,640 min and 5470 min, respectively. As the internal developmental stage at two different temperatures, it distinctly delimited based on their morphological characteristics. The internal developmental stages, identified by morphological characteristics, were subdivided into four distinct phases, improving the precision of post-mortem interval estimation. In this study, we attempted to delimit the pupae into developmental instars based on internal development with the help of X-ray imaging. More economical and easier than those currently in use, such as anatomical and micro-computed tomography. However, it is followed by collecting data through additional experiments at various temperatures to apply this method into actuality.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis of Eco-Friendly Nanostructured Lipid Carriers Decorated With Magnetic Nanoparticle Encapsulated Sesbania sesban Extract Against Vector Borne Culex pipiens (Diptera: Culicidae) and Musca domestica (Diptera: Muscidae) as Green Insecticides 磁性纳米颗粒包膜田菁提取物修饰的环保纳米结构脂质载体的合成及其对媒介传播的库蚊(双翅目:库蚊科)和家蝇(双翅目:蝇科)绿色杀虫剂的防治作用
IF 1.2 4区 农林科学
Entomological Research Pub Date : 2025-03-13 DOI: 10.1111/1748-5967.70029
Ibrahim Taha Radwan, Noha Bagato, Maysa M. Hegazy, Mohamed M. Baz, Hattan S. Gattan, Mohammed H. Alruhaili, Abadi M. Mashlawi, Abeer Mousa Alkhaibari, Saeed M. Alasmari, Abdelfattah Selim
{"title":"Synthesis of Eco-Friendly Nanostructured Lipid Carriers Decorated With Magnetic Nanoparticle Encapsulated Sesbania sesban Extract Against Vector Borne Culex pipiens (Diptera: Culicidae) and Musca domestica (Diptera: Muscidae) as Green Insecticides","authors":"Ibrahim Taha Radwan,&nbsp;Noha Bagato,&nbsp;Maysa M. Hegazy,&nbsp;Mohamed M. Baz,&nbsp;Hattan S. Gattan,&nbsp;Mohammed H. Alruhaili,&nbsp;Abadi M. Mashlawi,&nbsp;Abeer Mousa Alkhaibari,&nbsp;Saeed M. Alasmari,&nbsp;Abdelfattah Selim","doi":"10.1111/1748-5967.70029","DOIUrl":"https://doi.org/10.1111/1748-5967.70029","url":null,"abstract":"<div>\u0000 \u0000 <p>Mosquito-borne diseases remain a significant health concern amidst current microbial outbreaks. Phytochemicals offer environmentally safe, biodegradable, and targeted pest management. Nanostructure lipid carriers (NLCs), a second generation of solid lipid nanoparticles, are gaining attention as potential diagnostic and therapeutic tools. Sesbania leaves, rich in fatty acids, phenolics, and terpenes, were analyzed using gas chromatography–mass spectrometry. Magnetic nanoparticles (Se-NLC-MNPs) modified the surface of Sesbania extract, encapsulated in the NLC. The resulting nanoparticles were 129.2 and 218.5 nm in size, with zeta potentials of −6.20 and 43.9 mV, respectively. Transmission electron microscopy showed spherical and oval shapes. XRD patterns confirmed the successful decoration of the NLC with the magnetic nanoparticles. The Sesbania extract (Se) and its nanoparticle conjugates were tested for larvicidal efficacy against <i>Culex pipiens</i> and <i>Musca domestica</i> larvae, at doses ranging from 50 to 1500 ppm and 0.1 to 5 mg/mL. Se-NLC-MNPs showed higher larval mortality rates compared to their Se formulation extracts, achieving 100% mortality in third-instar larvae. Sesbania methanol extract contained more terpenes, fatty acids, and other organic compounds than the aqueous extract, making it more harmful to insect larvae. In terms of relative toxicity, Se-NLC-MNPs were more effective than Se-NLC. An in vitro cytotoxicity assay against the WI38 cell line indicated the cytotoxicity assay, suggesting the potential for these nanoparticles to develop into high-performance, environmentally acceptable therapeutics for mosquito-borne diseases.</p>\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"55 3","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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