Entomological Research最新文献_第3页

Trehalose and Glycogen Dynamics in Galleria mellonella Under Stress: Tissue-Specific Insights 海藻糖和糖原在压力下的变化：组织特异性的见解

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-23 DOI: 10.1111/1748-5967.70134

Tülay Turgut Genç, Serhat Kaya

{"title":"Trehalose and Glycogen Dynamics in Galleria mellonella Under Stress: Tissue-Specific Insights","authors":"Tülay Turgut Genç, Serhat Kaya","doi":"10.1111/1748-5967.70134","DOIUrl":"https://doi.org/10.1111/1748-5967.70134","url":null,"abstract":"Insects employ flexible metabolic strategies to cope with acute environmental stress. This study investigates how different stressors, including physical, mechanical, and thermal, influence carbohydrate metabolism in Galleria mellonella larvae. Trehalose and glucose levels were measured in hemolymph, together with trehalose, glycogen, and glucose levels in fat body tissues, at 4 and 12 h poststress to assess time- and tissue-specific metabolic shifts. All stress types triggered a rapid decline in hemolymph trehalose, emphasizing its role as a key early energy buffer under stress. Bead injection induced the most pronounced and persistent metabolic alterations, including sustained glucose elevation, suggesting increased metabolic demand under prolonged internal stress. In contrast, mechanical and thermal stressors caused largely transient changes, with partial recovery of carbohydrate levels by 12 h. Fat body responses varied with stressor type; thermal and mechanical stress led to early glycogen depletion, whereas bead injection caused minimal glycogen utilization but promoted local trehalose accumulation, indicating differential energy mobilization strategies. Overall, G. mellonella exhibited a stressor-specific and temporally structured metabolic program characterized by early trehalose mobilization, followed by glycogen-associated metabolic adjustments. These results highlight the central role of the fat body in metabolic regulation and support the utility of G. mellonella as a model organism for studying insect physiology and energy homeostasis.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148615593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic Insights Into How Diet Shapes Gut Microbiota Composition and Function in Scale Insects 饮食如何塑造蚧虫肠道微生物群组成和功能的宏基因组学见解

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-20 DOI: 10.1111/1748-5967.70133

Ji-Mu Lv, Xing-Jie Liu, Xin-Yi Zheng, Cheng-Yang Ding, Xiao-Nan Chen, Shun-Hai Yu, Yin-Feng Meng, Xu Wang, Yi-Xin Huang

{"title":"Metagenomic Insights Into How Diet Shapes Gut Microbiota Composition and Function in Scale Insects","authors":"Ji-Mu Lv, Xing-Jie Liu, Xin-Yi Zheng, Cheng-Yang Ding, Xiao-Nan Chen, Shun-Hai Yu, Yin-Feng Meng, Xu Wang, Yi-Xin Huang","doi":"10.1111/1748-5967.70133","DOIUrl":"https://doi.org/10.1111/1748-5967.70133","url":null,"abstract":"<div>\u0000 \u0000 Many insects harbor diverse gut microbiota, but the factors shaping these microbial communities remain insufficiently explored. To elucidate the structure and functions of gut microbiota in the scale insect Coronaproctus castanopsis and identify key influencing factors, we performed metagenomic sequencing on three scale insect species (C. castanopsis, Icerya purchasi, and Lecanodiaspis baculifera) with distinct dietary preferences. Results revealed bacteria dominating the microbial community (88.52%), primarily represented by phyla Bacteroidota and Pseudomonadota. At the genus level, Candidatus Walczuchella (55.47%) and Wolbachia (28.63%) were predominant. KEGG functional annotation showed that microbial genes were mainly associated with carbohydrate metabolism (335 genes), amino acid metabolism (249 genes), and membrane transport. Notably, gut microbiota diversity varied among samples, with Shannon indices ranging from 0.48 in nymph-stage C. castanopsis to 1.32 in adult-stage C. castanopsis. Additionally, specialized feeder C. castanopsis had fewer functional genes compared to generalist feeder I. purchasi (1790 fewer genes) and similarly feeding L. baculifera (200 fewer genes). These findings suggest that diet is an important factor associated with gut microbiota composition and gene functions in scale insects, although host phylogeny, developmental stage, and environmental factors may also contribute, offering novel insights into host–microbe interactions.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148615435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eight Microsatellite Markers for Genetic Analysis and Determination of Adaptation Range of Invasive Populations of the Species Hyphantria cunea Drury, 1773 (Erebidae, Lepidoptera) 8个微卫星标记对1773年入侵种群的遗传分析及适应范围的确定（Erebidae，鳞翅目）

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-15 DOI: 10.1111/1748-5967.70132

Tae Hwa Kang, Sun-Jae Park

{"title":"Eight Microsatellite Markers for Genetic Analysis and Determination of Adaptation Range of Invasive Populations of the Species Hyphantria cunea Drury, 1773 (Erebidae, Lepidoptera)","authors":"Tae Hwa Kang, Sun-Jae Park","doi":"10.1111/1748-5967.70132","DOIUrl":"https://doi.org/10.1111/1748-5967.70132","url":null,"abstract":"<div>\u0000 \u0000 The fall webworm (Hyphantria cunea), a North American native, is a globally invasive forest pest that causes periodic outbreaks and damages more than 400 plant species worldwide. Therefore, to support population structure and invasion pattern analyses, we developed eight microsatellite loci markers for invasive populations of H. cunea using next-generation sequencing (NGS). We identified 107,660 microsatellite loci in the 1,082,719,559 bp of genomic sequence. Among the loci, we firstly sorted 20 microsatellite loci according to the repeat sequence length and polymorphism of the target sequence, and then finally selected eight markers through a polymerase chain reaction (PCR) efficiency test. The eight selected markers were then tested for the presence of null alleles and PCR errors using four algorithms. We detected null alleles showing a frequency (p) ranging from 0.1456 to 0.2473; in microsatellite analysis, for p > 0.20, the mean “estimated with null” exclusion probability can be much higher than the “true” and “estimated without null” values. Therefore, we assessed the genetic diversity of an H. cunea population from the Icheon region of Korea (n = 20) using eight markers. Deviation from Hardy–Weinberg equilibrium (HWE) was not detected at any of the analyzed loci. Therefore, these eight markers may be useful for analyzing the genetic diversity and invasive routes of H. cunea populations in Korea.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex Ratios Within the Quasi-Social Parasitoid Genus Sclerodermus 拟社会性拟寄生物硬皮蝇属的性别比

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-12 DOI: 10.1111/1748-5967.70131

Serena Malabusini, Daniela Lupi, Ian C. W. Hardy

{"title":"Sex Ratios Within the Quasi-Social Parasitoid Genus Sclerodermus","authors":"Serena Malabusini, Daniela Lupi, Ian C. W. Hardy","doi":"10.1111/1748-5967.70131","DOIUrl":"https://doi.org/10.1111/1748-5967.70131","url":null,"abstract":"Sex ratios are an important component of parasitoid wasps' reproductive strategies and seem to be generally well described by evolutionary ecological theory, such as local mate competition (LMC). The sex ratios of several Sclerodermus species do not fit the predictions of standard LMC models, as their sex ratios remain strongly female biased irrespective of the number of foundress females contributing offspring to locally mating groups. Unusually among parasitoids, Sclerodermus are quasi-social: co-foundresses remaining in association with each other for long periods while communally producing and tending large broods of offspring on a single host. Modified LMC theory, considering that some co-foundresses may actively alter the sex ratios produced by other co-foundresses via physiological suppression or post-ovipositional infanticide, predicts that brood sex ratios at maturity will be relatively insensitive to foundress number, as observed within species. Here, we explore whether the within-species and with-study sex ratio patterns are also observed across studies and species, using data from 38 prior studies in a comparative analysis of the relationship between sex ratio and foundress number. We find that, like within-species observations, sex ratios increase very only mildly, or not at all, as foundress numbers increase, and are only slightly affected by host species and by wasp species identity: Sclerodermus brood sex ratios essentially remain at around 9% males. Current theory and current evidence are broadly akin but do not match exactly: This may be due to methodological constraints in our comparative analyses or to unexplored or unmodelled aspects of the biology of Sclerodermus.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Phylogenetic Reassessment of the Superfamily Eriophyoidea (Arachnida: Acari) Based on COI and 18S rRNA 基于COI和18S rRNA的棘螨超科（蛛形纲：蜱螨）分子系统发育再评价

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-07 DOI: 10.1111/1748-5967.70130

Jae Wook Jung, Ui Wook Hwang

{"title":"Molecular Phylogenetic Reassessment of the Superfamily Eriophyoidea (Arachnida: Acari) Based on COI and 18S rRNA","authors":"Jae Wook Jung, Ui Wook Hwang","doi":"10.1111/1748-5967.70130","DOIUrl":"https://doi.org/10.1111/1748-5967.70130","url":null,"abstract":"Gall mites (superfamily Eriophyoidea) are highly specialized phytophagous arthropods with extreme host plant specificity and marked morphological diversity. Despite extensive taxonomic and molecular research, phylogenetic relationships within the superfamily remain unresolved, largely due to persistent polyphyly among several families. We reassessed the molecular phylogeny of Eriophyoidea using mitochondrial cytochrome c oxidase subunit I (COI) and nuclear 18S rRNA genes. Field surveys at five sites in Korea yielded five eriophyoid taxa from five host plant species, including a putative new species of Acaricalus and the first Korean record of Tetra suspensae. From these specimens, we generated 11 COI and 6 18S rRNA gene sequences. Together with GenBank data, 181 COI and 179 18S rRNA gene sequences were analyzed using a maximum likelihood method. Both COI- and 18S-based phylogenies strongly supported the monophyly of Eriophyoidea. However, Phytoptidae was consistently divided into two different genetic lineages, and relationships among lower-level taxa showed extensive nonmonophyly. All species of Eriophyidae and Diptilomiopidae and some of Phytoptidae were frequently intermingled, indicating pronounced polyphyly at the family and subfamily levels. Although Korean eriophyoids generally showed concordance between morphological and molecular data, their placement within polyphyletic assemblages highlights limitations of current classifications. Our results underscore the need for integrative approaches combining broader taxon sampling, multilocus or phylogenomic data, detailed morphological reassessment, and ecological information, particularly host plant associations.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148462754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of CO2 Narcosis and Cold Storage on Colony Success of Artificially Inseminated Bumblebee Queens CO2麻醉和冷藏对人工授精大黄蜂蜂王群体成功的影响

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-01 DOI: 10.1111/1748-5967.70128

Hyung Joo Yoon, Kyeong Yong Lee, Su Jin Lee, Mi Rye Choi, Kwang Sik Lee, Byung Rae Jin

{"title":"Effects of CO2 Narcosis and Cold Storage on Colony Success of Artificially Inseminated Bumblebee Queens","authors":"Hyung Joo Yoon, Kyeong Yong Lee, Su Jin Lee, Mi Rye Choi, Kwang Sik Lee, Byung Rae Jin","doi":"10.1111/1748-5967.70128","DOIUrl":"https://doi.org/10.1111/1748-5967.70128","url":null,"abstract":"<div>\u0000 \u0000 Bumblebees are vital pollinators for both agricultural crops and natural plants. Among the various bumblebee species, Bombus terrestris (Hymenoptera: Apidae) is available commercially for pollination purposes. To develop reliable artificial insemination techniques for bumblebees, we examined the effects of different carbon dioxide (CO2) treatments and cold storage after insemination on colony success of bumblebee queens. CO2 treatments were applied to queens as follows: CO2-1 (CO2 narcosis once during insemination) and CO2-2 (CO2 narcosis twice during insemination and 3 days after insemination). Queens inseminated under CO2-1 conditions were subjected to cold storage at 2.5°C for 4, 8, and 12 weeks. In the experiments, the insemination and survival rates of queens after insemination were almost 100%. In the CO2 treatments, oviposition by inseminated queens and adult emergence rates exhibited similar patterns in the CO2-1 and CO2-2 conditions but declined gradually as cold storage duration increased. Among the bumblebee species examined, the efficiency of artificial insemination, as measured by adult emergence, was the highest in B. terrestris, followed by B. ignitus and B. hypocrita sapporoensis. To evaluate the suitability of B. terrestris queens for artificial insemination with semen from different Bombus species, queens were inseminated with semen from B. ignitus and B. hypocrita sapporoensis, and B. terrestris males were produced in all treatments. The results provide a basis for artificial insemination in bumblebees and could have various applications.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Biocontrol of Mindarus japonicus Takahashi, a New Pest of Abies koreana E.H.Wilson Using Bacillus velezensis CE 100 韩国冷杉新害虫高桥日本明darus japonicus Takahashi的鉴定与利用velezensis c100进行生物防治

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-07-01 DOI: 10.1111/1748-5967.70129

Su-In Choi, Jae-Hyun Moon, Vantha Choub, Ju-Yeol Yun, Su-Yeon Lee, Henry B. Ajuna, Iksoo Kim, Hyo-In Lim, Young Sang Ahn

{"title":"Identification and Biocontrol of Mindarus japonicus Takahashi, a New Pest of Abies koreana E.H.Wilson Using Bacillus velezensis CE 100","authors":"Su-In Choi, Jae-Hyun Moon, Vantha Choub, Ju-Yeol Yun, Su-Yeon Lee, Henry B. Ajuna, Iksoo Kim, Hyo-In Lim, Young Sang Ahn","doi":"10.1111/1748-5967.70129","DOIUrl":"https://doi.org/10.1111/1748-5967.70129","url":null,"abstract":"Subalpine conifers such as Abies koreana E.H.Wilson (Korean fir) play a significant ecological role but are increasingly endangered due to the effects of climate change, including the high prevalence of insect pests. This study identified for the first time, a notorious aphid pest on newly developed needles and twigs of A. koreana, causing leaf curling, clustering, and stunted growth. The pest was identified as Mindarus japonicus Takahashi based on the morphological features and molecular analysis of mitochondrial barcode region. Due to restrictions on the use of chemical pesticides in forest environments, this study investigated the aphicidal effectiveness of Bacillus velezensis CE 100 as a prospective biocontrol strategy against M. japonicus. During growth, B. velezensis CE 100 produced cuticle-degrading enzymes: lipase, protease, and chitinase in the bacterial broth culture to a peak activity of 148.74, 45.12, and 77.08 units/mL, respectively. Treatment with the bacterial broth of B. velezensis CE 100 caused 75.00% mortality after 48 h. The crude enzyme fraction showed specific enzyme activity of 9398.66, 716.21, and 508.00 units/mg protein for lipase, protease, and chitinase, respectively, and caused 87.50% aphid mortality. Ultrastructural analysis using scanning and transmission electron microscopy revealed that both treatments caused extensive damage to the epicuticle, including cracking and pore formation, collapsed, and disorientation of the internal cuticle layers. The findings demonstrate the prospect of applying B. velezensis CE 100 as an eco-friendly strategy in the management of M. japonicus, a newly identified pest of A. koreana, to enhance the conservation of subalpine conifers.","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148358103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Red Light Increases the Crawling Distance of Arma Chinensis and Halyomorpha halys and Their Visual Protein Identification 红光增加中国剑草和Halyomorpha halys爬行距离及其视觉蛋白鉴定

IF 1.3 4区农林科学

Entomological Research Pub Date : 2026-06-15 DOI: 10.1111/1748-5967.70127

Yu Chen, Xiaoyu Yan, Dianyu Liu, Yichen Wang, Zhihan Su, Wenyan Xu, Xiaolin Dong, Yanfang Pei, Chenxi Liu

{"title":"Red Light Increases the Crawling Distance of Arma Chinensis and Halyomorpha halys and Their Visual Protein Identification","authors":"Yu Chen, Xiaoyu Yan, Dianyu Liu, Yichen Wang, Zhihan Su, Wenyan Xu, Xiaolin Dong, Yanfang Pei, Chenxi Liu","doi":"10.1111/1748-5967.70127","DOIUrl":"https://doi.org/10.1111/1748-5967.70127","url":null,"abstract":"<div>\u0000 \u0000 Phototactic behavior is critical for insect ecological adaptation, especially visual responses to light wavelengths. This study examined locomotor responses to red and ultraviolet (UV) light in congeneric pentatomids, a predator (Arma chinensis) versus a pest (Halyomorpha halys), and explored the underlying visual molecular mechanisms. Results showed that red and UV light significantly increased total movement distance of both species with distinct species—and sex-specific differences. Red light exerted the strongest promoting effect on total movement distance, particularly during 4–8 h of exposure. Male A. chinensis traveled farther, moved faster, and paused more frequently than females under red and UV light. Male H. halys moved faster and paused more frequently under red light; H. halys moved faster than A. chinensis under red light, while female H. halys had longer pause duration than female A. chinensis under UV light. For H. halys, females traveled farther under control light, with no sex difference under red light and males traveling farther under UV light. H. halys possesses three long-wavelength opsins versus two in A. chinensis. Both species share the conserved 7tm_photoreceptors_insect domain and typical GPCR characteristics, and their opsins are highly homologous with other Hemiptera. Although conducted under laboratory conditions, our findings provide valuable insights into the development of environmentally friendly pest control methods and open new avenues for basic and applied insect visual ecology research.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Secondary Endosymbiotic Bacterial Infections in Harvestman: Diversity and Evolutionary Dynamics 研究收割机继发性内共生细菌感染：多样性和进化动力学

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-06-12 DOI: 10.1111/1748-5967.70125

Ruoyi Xiao, Chao Zhang, Hongmei Li, Hongkang Xv, Feng Zhang, Likun Zhao

{"title":"Investigating Secondary Endosymbiotic Bacterial Infections in Harvestman: Diversity and Evolutionary Dynamics","authors":"Ruoyi Xiao, Chao Zhang, Hongmei Li, Hongkang Xv, Feng Zhang, Likun Zhao","doi":"10.1111/1748-5967.70125","DOIUrl":"https://doi.org/10.1111/1748-5967.70125","url":null,"abstract":"<div>\u0000 \u0000 The diversity of secondary endosymbiotic bacteria plays an important role in host biology. Our recent findings revealed multiple secondary endosymbiotic infections in harvestmen, particularly involving Wolbachia and Rickettsiella, which have been rarely reported in this group. In this study, we found that Sinonychia martensi populations from six of the seven caves sampled were positive for Wolbachia, and one cave population was infected with Rickettsiella. Additionally, 12 of the 14 surface-dwelling harvestmen populations showed varying levels of infection with Wolbachia and Rickettsiella, while Cardinium was detected in only one population. Both cave and surface-dwelling harvestmen exhibited variable levels of secondary endosymbiotic infection, and multiple infections were common. Comparative phylogenetic analyses, population structure analyses of cave-dwelling harvestmen, and recombination analyses suggested that the acquisition of secondary endosymbionts did not result from host-symbiont co-divergence. Instead, horizontal transmission and genetic recombination appear to have played significant roles in their evolutionary history.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 6","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Third Instar Black Soldier Fly Larvae on the Biodegradability and Characteristics of Pig Manure–Sawdust Mixtures Across Different Ratios 3龄黑虻幼虫对不同比例猪粪-木屑混合物生物降解性及特性的影响

IF 1.6 4区农林科学

Entomological Research Pub Date : 2026-06-08 DOI: 10.1111/1748-5967.70126

In-Hag Choi

{"title":"Effects of Third Instar Black Soldier Fly Larvae on the Biodegradability and Characteristics of Pig Manure–Sawdust Mixtures Across Different Ratios","authors":"In-Hag Choi","doi":"10.1111/1748-5967.70126","DOIUrl":"https://doi.org/10.1111/1748-5967.70126","url":null,"abstract":"<div>\u0000 \u0000 Black soldier fly (BSF) larvae have attracted significant attention as an innovative and sustainable solution for biodegradation and reduction of organic waste. Importantly, their utilization offers an ecologically viable and efficient strategy for waste management, especially in enhancing the composting process. Therefore, this study aimed to investigate the effects of third instar BSF larvae on the biodegradation and physicochemical attributes of pig manure and sawdust mixtures at varying ratios to identify the optimal conditions for improving compost quality. In terms of biodegradability, BSF (0.5 kg) larvae cultivated on a 1:1 pig manure (2.5 kg) to sawdust (2.5 kg) ratio (T2 group) did not exhibit superior biodegradation ability compared with the control and T1 (a mixture of 3 kg pig manure and 2 kg sawdust + 0.5 kg BSF larvae) groups. However, the T2 group demonstrated considerable potential as a supplementary substrate for rearing BSF larvae, yielding notable improvements in composting efficiency. In addition, key parameters, such as total carbon and nitrogen contents, fiber component, and C:N ratio were more favorable in the T2 group than in the other groups. Importantly, the C:N ratio (15) and low pH of the T2 group were consistent with the requirements for effective composting. Collectively, these results highlight the importance of optimizing substrate composition for effective composting. However, further research is required to explore the mechanisms driving waste reduction and identify the optimal levels of fiber components for BSF larvae in pig manure and sawdust mixtures.\u0000 </div>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"56 6","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148237432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0