Entomological Research最新文献

{"title":"In vivo efficacy evaluation of white-spotted flower chafer (Protaetia brevitarsis seulensis) extract for improving blood circulation","authors":"Jin-Sung Son,&nbsp;Tae-Ho Chung","doi":"10.1111/1748-5967.12754","DOIUrl":"https://doi.org/10.1111/1748-5967.12754","url":null,"abstract":"<p>Recent studies have suggested the use of insects to enhance various bioactivities in the treatment of many diseases or as alternative sources of nutrients. White-spotted flower chafer (<i>Protaetia brevitarsis seulensis</i>; PBS) extract, which is used for medicinal purposes, appears to be effective in improving blood circulation. However, few studies have investigated the efficacy of PBS extract in improving blood circulation. Here, we investigated the effects of PBS extract on serum cholesterol metabolites (total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol) and anticoagulant activities (prothrombin time [PT], activated partial thromboplastin time [aPTT], and fibrinogen [Fib]) in very-high-fat diet (VHFD)-induced hyperlipidemic rats. The experimental groups comprised a control group that was not treated with PBS and five PBS treatment groups (50, 100, 150, 200, and 250 mg/kg) in a randomized complete block design. In the efficacy evaluation for health functionality to reduce cholesterol levels, no beneficial effects on serum cholesterol metabolites were observed in the control and PBS treatment groups. In addition, the cholesterol data indicated that the effect of the PBS extract was not related to antioxidant activity. Moreover, the PT, aPPT, and Fib values were prolonged by increasing the PBS extract concentration, and PBS extract concentrations of 150–250 mg/kg were recommended for the VHFD model based on the anticoagulant activity. In conclusion, the PBS extract can be used in a potential therapeutic strategy for improving anticoagulant activity rather than improving the serum cholesterol profile.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 6","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141488393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression characterization of miR-6826-3p in Pardosa pseudoannulata (Araneae: Lycosidae) under high temperature stress","authors":"Haoran Zhang,&nbsp;Di Fu,&nbsp;Qing Yang,&nbsp;Feng Xiao,&nbsp;Meimei Mu,&nbsp;Rong Xiao,&nbsp;Daochao Jin","doi":"10.1111/1748-5967.12755","DOIUrl":"https://doi.org/10.1111/1748-5967.12755","url":null,"abstract":"<p><i>Pardosa pseudoannulata</i> is key predator in rice ecosystems; it plays a crucial role in rice pest biological control, effectively managing major rice pests such as rice planthopper. At present, there are few related studies on spiders' microRNA (miRNA). Consequently, we initiate a study on miR-6826-3p in <i>P. pseudoannulata</i> in response to high temperatures. First, we identified a total of 686 target genes of miR-6826-3p, 439 target genes had annotated results in the database, primarily involved in transcription and metabolism functions. Subsequently, quantitative real-time PCR was employed to assess the relative expression levels of miR-6826-3p in <i>P. pseudoannulata</i> under different temperatures (31, 34, 37, 40°C) and various durations (1, 2, 4, 8, 12 h). The results revealed that compared to the control group (25°C), the relative expression level of miR-6826-3p in female spiders significantly increased under the same temperature and different duration stresses. The expression level of miR-6826-3p in male spiders showed an initial increase followed by a decrease trend, with relatively lower expression levels after 1 and 12 h at the same temperature conditions. Furthermore, under the same duration of stress, compared to the control group (25°C), the relative expression level of miR-6826-3p in female spiders significantly increased at different temperatures. These results demonstrate that <i>P. pseudoannulata</i> miR-6826-3p plays a significant role in response to high-temperature stress. The research findings can provide a basis for future in-depth studies on the miRNA functional of <i>P. pseudoannulata</i>, aiding in the elucidation of the regulatory mechanisms of <i>P. pseudoannulata</i> miRNAs in response to high-temperature environments.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression profiles and phylogenetic properties of venom gland-specific viruses in some aculeate bees and wasps","authors":"Kyungjae Andrew Yoon,&nbsp;Woo Jin Kim,&nbsp;Hee Jong Shin,&nbsp;Si Hyeock Lee","doi":"10.1111/1748-5967.12752","DOIUrl":"https://doi.org/10.1111/1748-5967.12752","url":null,"abstract":"<p>To identify viruses and compare their abundance levels in the venom glands of hymenopteran species, we conducted venom gland-specific transcriptome assemblies and analyses of 22 aculeate bees and wasps and identified the RNA genomes of picornaviruses. Additionally, we investigated the expression patterns of viruses in the venom glands over time following capture. Honeybee-infecting viruses, including the black queen cell virus (BQCV), the deformed wing virus (DWV) and the Israeli acute paralysis virus (IAPV), were highly expressed in the venom glands of <i>Apis mellifera</i> and social wasps. This finding suggests that the venom of bees and wasps is likely to contain these viruses, which can be transmitted horizontally between species through stinger use. <i>Apis mellifera</i> exhibited an increasing pattern of abundance levels for BQCV, DWV, IAPV and <i>Triatovirus</i>, whereas the social wasp <i>Vespa crabro</i> showed increasing abundance levels of IAPV and <i>Triatovirus</i> over different capture periods. This suggests that the venom glands of honeybees and wasps may provide suitable conditions for active viral replication and may be an organ for virus accumulation and transmission. Some viral sequences clearly reflected the phylogeny of aculeate species, implying host-specific virus evolution. On the other hand, other viruses exhibited unique evolutionary patterns of phylogeny, possibly caused by specific ecological interactions. Our study provides insights into the composition and evolutionary properties of viral genes in the venom glands of certain aculeate bees and wasps, as well as the potential horizontal transmission of these viruses among bee and wasp species.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12752","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141298474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance and nutritional profile of Tenebrio molitor raised on a diet composed of livestock feedstuff","authors":"Jun Ho Cho,&nbsp;Ho Am Jang,&nbsp;Yong Seok Lee,&nbsp;Yeon Soo Han,&nbsp;Yong Hun Jo","doi":"10.1111/1748-5967.12750","DOIUrl":"https://doi.org/10.1111/1748-5967.12750","url":null,"abstract":"<p>Edible insects such as the mealworm (<i>Tenebrio molitor</i>) are emerging as an alternative animal protein resource to meet the needs of a growing global population. However, despite the successful mass production of edible insects, research into practical compound feed for edible insect is still insufficient. In this study, mealworms were fed wheat bran, layer chicken feed mash, chicken pellets and cattle pellets for 1 to 36 days, after which their body length was measured. In the groups fed with chicken feed mash, chicken pellets or cattle pellets, the mean body lengths were 2.88, 2.92 and 2.95 cm, respectively, similar to the 2.95 cm of the control <i>T. molitor</i> fed with wheat bran. Individual weight measurements yielded similar results: control, 7.87 g; chicken feed mash, 7.43 g; chicken pellets, 7.60 g; and cattle pellets, 7.63 g. Analysis of livestock feed intake showed that the intake was 290.4 g in control insects, 137.7 g in insects fed chicken feed mash, 220.2 g in insects fed chicken pellets and 230.3 g in insects fed cattle pellets. In the excretion analysis, the excretion of the control insects (161.60 g) was larger than that of the insects fed with chicken feed mash (93.37 g), chicken pellets (93.40 g) or cattle pellets (133.87 g). An analysis of the nutritional content of <i>T. molitor</i> based on nine major nutrients showed no significant differences between the control and the test groups. These results can be used to improve the price competitiveness and quality of <i>T. molitor</i> for commercial development.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12750","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population tracing analysis and overwintering of Bactrocera dorsalis (Hendel) in newly occurring areas in China","authors":"Yan Zhao,&nbsp;Sandeep Singh,&nbsp;Xiaodong Zhang,&nbsp;Mingqing Shang,&nbsp;Yujia Qin,&nbsp;Zihua Zhao,&nbsp;Chunxian Lu,&nbsp;Mai Lu,&nbsp;Zhihong Li","doi":"10.1111/1748-5967.12749","DOIUrl":"https://doi.org/10.1111/1748-5967.12749","url":null,"abstract":"<p><i>Bactrocera dorsalis</i> (Hendel), a very destructive insect pest of many fruits and vegetables, is widespread in the tropical and subtropical zones of Asia. However, the northward extension of <i>B. dorsalis</i> has been reported in China recently. It is crucial to determine the origin of new populations and to investigate the invasive potential of the species. Here, we investigated <i>B. dorsalis</i> in Nanyang, Henan Province and Heze, Shandong Province using a combined methodological approach. We first detected the hydrogen stable isotope ratios (δ²H) of <i>B. dorsalis</i> samples. The results showed that the δ²H values for <i>B. dorsalis</i> from Nanyang were consistent with those of the local rainwater. In contrast, the samples from Heze were inconsistent with those of the rainwater in Heze. We then sequenced the complete mitochondrial cytochrome oxidase I (mtDNA COI) from the same fruit fly samples for haplotype diversity and phylogenetic analyses. We found a close genetic relationship between the Heze population and Nanyang, Hefei, Sanya, Fuyang and Wuhan populations. Besides, we surveyed the fruit's origin in the wholesale market, and concluded that <i>B. dorsalis</i> trapped in Heze might have come from Hainan and Zhejiang in China. Finally, overwintering potential experiments showed that a small number of pupae could survive during the test periods in Nanyang, whereas no pupae could survive in Heze. Overall, this study reveals that the Nanyang population of <i>B. dorsalis</i> were resident, whereas the Heze population did not colonize and were repeated entries from other areas every spring.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of aqueous and alcoholic formulations extracted from Padina boryana algae against Aedes aegypti mosquito larvae: A comparative study","authors":"Hanan S. Alyahya","doi":"10.1111/1748-5967.12748","DOIUrl":"https://doi.org/10.1111/1748-5967.12748","url":null,"abstract":"<p><i>Aedes aegypti</i> is a mosquito species known for transmitting several diseases, with dengue fever being of utmost importance. Controlling the spread of dengue fever remains a significant global health challenge. Other diseases transmitted by <i>Ae. aegypti</i> include Zika virus, chikungunya, and yellow fever. <i>Padina boryana</i> algae were collected from the Red Sea coast south of Jeddah to evaluate the effectiveness of its alcoholic and aqueous extracts against <i>Ae. aegypti</i> mosquito larvae. The algae sample was washed, dried in the shade, processed, and subjected to aqueous and alcohol extraction using a rotary evaporator. The resulting extracts were tested for their larvicidal activity against <i>Ae. aegypti</i> mosquito larvae using standard bioassay techniques. The larval mortality rates were recorded, and the lethal concentration (LC₅₀ and LC₉₀) values were calculated.</p><p>The results revealed that both the aqueous and alcoholic extracts of <i>P. boryana</i> algae exhibited significant larvicidal activity against <i>Ae. aegypti</i> mosquito larvae. However, the alcoholic formulation demonstrated higher efficacy compared to the aqueous formulation. The LC₅₀ values for the alcoholic and aqueous extracts were 194.8 and 195.7 ppm, respectively. The LC₉₀ values for both extracts were 653.1 and 656.7 ppm, respectively. This indicates the effectiveness of <i>Padina</i> extracts as an effective pesticide against the larvae of the <i>Ae. aegypti</i> mosquito. Overall, the alcohol extract showed promising efficacy, indicating its potential as a significant component in developing effective mosquito control strategies.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141182218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of uridine diphosphate glycosyltransferases in Dioryctria abietella, with an emphasis on putative roles in olfaction and reproduction","authors":"Dan-Lu Chen,&nbsp;Ning-Na Yin,&nbsp;Peng-Fei Wang,&nbsp;Dan Shen,&nbsp;Chun Wu,&nbsp;Nai-Yong Liu","doi":"10.1111/1748-5967.12734","DOIUrl":"https://doi.org/10.1111/1748-5967.12734","url":null,"abstract":"<p>Multiple physiological processes are involved in the interactions between the herbivore <i>Dioryctria abietella</i> and Pinaceae plants, with a key adaptation being the metabolic detoxification of host plant defensive substances. Moreover, the synthetic insecticides applied to control this coneworm are also shaping genes related to detoxification, such as the uridine diphosphate (UDP) glycosyltransferase (UGT) genes. Yet, the molecular mechanisms underpinning the resistance of <i>D. abietella</i> to toxic compounds remain unknown. In this study, we present an important UGT gene family involved in the detoxification mechanisms of <i>D. abietella</i>. Combining bioinformatic and transcriptomic approaches, a total of 37 UGT-coding genes were identified from the transcriptome of <i>D. abietella</i>, with 20 full-length sequences that shared high homology with UGTs found in other pyralid moths. These DabiUGTs were phylogenetically clustered into 12 subfamilies, with the three small clusters in the UGT33 and UGT40 clades mainly contributing to the size of the UGT gene repertoire in <i>D. abietella</i>. Expression profiles obtained from RNA sequencing (RNA-Seq), reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qPCR) revealed that over half of <i>DabiUGT</i>s had a broad tissue expression profile, with 28, 28, 31 and 35 genes detected separately in antennae, legs, abdomens and reproductive tissues. Notably, <i>DabiUGT20</i>, <i>DabiUGT25</i>, <i>DabiUGT28</i> and <i>DabiUGT34</i> were all significantly enriched in the antennae. Several <i>DabiUGT</i>s had significant expression levels in reproductive tissues, including <i>DabiUGT6</i>, <i>DabiUGT9</i>, <i>DabiUGT24</i>, <i>DabiUGT26</i> and <i>DabiUGT32</i> in female accessory glands, <i>DabiUGT15</i> in male accessory glands and <i>DabiUGT25</i> in male testes. Altogether, our results identify candidate <i>DabiUGT</i>s for mediating olfaction and reproduction in <i>D. abietella</i>, warranting further investigation into the roles of <i>DabiUGT</i>s in pesticide resistance, odorant detection and reproduction.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141085028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marching towards bioeconomy: Larvicidal effect of three indigenous plant extracts from Sri Lanka—Garcinia quaesita, Garcinia zeylanica, and Coleus hadiensis on dengue vector Aedes aegypti","authors":"H.A.K. Ranasinghe,&nbsp;E.H.L. Perera,&nbsp;G.C.A. Perera,&nbsp;J.D.A.S.M. Jayakodi,&nbsp;J.A.H. Madhumika,&nbsp;M.S. Ishara,&nbsp;M. Hettihewa","doi":"10.1111/1748-5967.12732","DOIUrl":"https://doi.org/10.1111/1748-5967.12732","url":null,"abstract":"<p>Bioeconomy would be a beneficial solution for funding through the rational use of finances and human resources in order to achieve better productivity and fruitfulness of mosquito control programs. Synthetic insecticides are widely used to repress mosquitoes. However, it instigates problems such as insecticide resistance among mosquitoes such as developing resistance, bio-accumulation, and ecosystem destabilization. Natural plant-based products are a healthy alternative to use as mosquito larvicides. The country has to spend a considerable cost for buying synthetic insecticides too. Therefore, the present study was undertaken to evaluate larvicidal efficacy of <i>Garcinia zeylanica</i>, <i>Garcinia quaesita</i>, and <i>Coleus hadiensis</i> against <i>Aedes aegypti</i> mosquito larvae. Fresh leaves of plants were collected, and the aqueous crude extract was prepared. Phytochemicals were extracted using refluxing technique. A concentration series of crude extract of leaves were prepared separately from 20 to 100 mg/L. Batches of each containing 100 third instar larvae of <i>Ae. aegypti</i> were used for larval bioassays. <i>Ae. aegypti</i> mosquito larvae were evaluated in accordance with the guidelines of World Health Organization. The experimental setup was repeated four times at each concentration. Probit analysis was used to evaluate the relationship of mortality with the concentration of aqueous crude extract. The <i>G. zeylanica</i> and <i>G. quaesita</i> leaf extracts showed a dose-dependent effect against <i>Ae. aegypti</i> larvae after the 24 and 48 h exposure period. Interestingly, <i>C. hadiensis</i> did not show any dose-dependent effect against <i>A. aegypti</i> mosquito larvae. The percentage mortality rates have shown a significant variance among different concentrations (<i>P</i> = 0.000). The recorded LC₅₀ and LC₉₀ for aqueous crude extract of <i>G. zeylanica</i> were 27.167 and 52.861 g/L, respectively, and LC₅₀ and LC₉₀ for aqueous crude extract of <i>G. quaesita</i> were 36.841 and 76.036 g/L, respectively, after 24 h of exposure period. The <i>G. zeylanica</i> and <i>G. quaesita</i> plant's high larvicidal activity is supported by the presence of phytochemicals such as saponins, steroids, flavonoids, and phenol, which showed combination effects in terms of larvicidal action to mosquito larvae. Hence, there is a potential of <i>G. zeylanica</i> and <i>G. quaesita</i> aqueous leaf extracts as a key source for the development of an environment-friendly plant-based larvicide against <i>Ae. aegypti</i>. Hence, this warrants vector control entities to re-think “biological wealth for economic prosperity” with environmentally friendly country-wide control approaches for medically important disease vectors.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141085029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genomic investigation on the origins of the Korean brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphasidae)","authors":"Suyeon Jeong,&nbsp;Haechan Gill,&nbsp;Taeuk Yu,&nbsp;Jinho Na,&nbsp;Hohun Ki,&nbsp;Hyun-Na Koo,&nbsp;Gil-Hah Kim,&nbsp;Dongyoung Kim,&nbsp;Sung-Hwan Jo,&nbsp;Choongwon Jeong,&nbsp;Soowon Cho","doi":"10.1111/1748-5967.12722","DOIUrl":"https://doi.org/10.1111/1748-5967.12722","url":null,"abstract":"<p><i>Nilaparvata lugens</i>, the brown planthopper (BPH), is a serious pest species. BPHs cause significant damage to rice plants in Korea as well as other countries in East and Southeast Asia. As BPHs cannot survive winter in Korea, they annually migrate into Korea from foreign countries. The BPHs found in Korea are believed to originate from China, but most BPHs in China are also known to originate from Southeast Asia. To understand the origin of Korean BPHs, we conducted a genotyping-by-sequencing (GBS) study. We sampled BPHs from five locations in Korea and five countries in Southeast Asia and analyzed the GBS and sequencing results using various methods based on the <i>f</i> statistics and admixture graph analyses. We confirmed that the domestic BPHs shared a greater genetic drift than the BPHs from Southeast Asian populations over several years, implying that a continuous genetic substratum of Korea exists. This genetic substratum is genetically closer to BPHs from the southern part (Thailand and Cambodia) of Southeast Asia than to BPHs from the northern part (Bhutan, Myanmar and Laos) of Southeast Asia. In addition, direct gene flows from Southeast Asia seem possible, so Korea is considered a hotspot where diverse BPH populations mix. Therefore, the origin of Korean BPHs extends beyond China and as far as southern Southeast Asia. This result will help to understand and control the population dynamics of the Korean BPH population.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12722","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140952696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of microemulsion of some insecticides and evaluating their efficacy against cotton leaf worm on cotton crop under laboratory and field conditions","authors":"Aly Derbalah,&nbsp;Saad Morsy,&nbsp;El-Zahi S. El-Zahi,&nbsp;Mohmmed Zidan,&nbsp;Nawal Abdulaziz Alfuhaid,&nbsp;Amany Hamza,&nbsp;Sherif Abdeldayem","doi":"10.1111/1748-5967.12733","DOIUrl":"https://doi.org/10.1111/1748-5967.12733","url":null,"abstract":"<p>Traditional pesticide formulations have some shortcomings, including limited efficacy and the usage of huge amounts of insecticide, which has a negative impact on the environment. Using nano-pesticide formulations reduces the amount of pesticide applied, minimizing pest control expenses and environmental contamination. As a result, this study was conducted to develop a microemulsion formulation containing emamectin benzoate or beta-cyfluthrin with improved morphology, droplet size, emulsion stability, and persistence of foam. Moreover to assess the efficacy of the developed microemulsion in comparison to the convention alone against the Egyptian cotton leaf worm, <i>Spodoptera littoralis</i> (Boisd.) under both laboratory and field conditions. The results showed that the optimum conditions for emamectin benzoate microemulsion was 2% active ingredient, 5% (w/w) ethyl acetate, and DMF (3:2), 3.5% (w/w) Geronol ff4 and Etocas 35 (6:1), 5% (w/w) ethanol, and 86.5% (w/w) water, while for beta-cyfluthrin, a 5.0% active ingredient, 15% (w/w) Solvesso 100 and ethyl acetate (2:1), 7.0% (w/w) Geronol ff4 and Etocas 35 (6:1), 10% (w/w) ethanol, and 68% (w/w) water. Under laboratory conditions, emamectin benzoate was more toxic than beta-cyfluthrin to <i>Spodoptera littoralis</i> second and fourth instar larvae. Emamectin benzoate was more effective that beta-cyfluthrin against <i>Spodoptera littoralis</i> in the field, whether as a microemulsion or a commercial product. Thus, microemulsions of tested insecticides with better formulation qualities may be more effective than traditional ones in controlling cotton leaf worm.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 5","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}