农林科学最新文献

{"title":"Pathogenicity and transmissibility differences in goose-originated subgenotype 3 duck Tembusu virus in goose embryos and 3-day-old goslings.","authors":"Ziqiao Zhao, Junhong Ke, Mengfan Liu, Zuoxin Chen, Shuwen Li, Wuque Wei, Kun Mei, Shujian Huang","doi":"10.1080/03079457.2024.2373365","DOIUrl":"10.1080/03079457.2024.2373365","url":null,"abstract":"<p><p>The Duck Tembusu virus (DTMUV) was first reported in China in 2010 and has since caused substantial economic losses in the poultry breeding industry. In the autumn of 2022, an outbreak of an infectious disease resembling DTMUV was reported in Guangdong Province, China, which caused significantly high mortality in goose embryos, and decreased egg production. This study identified one strain of the new subgenotype 3 of DTMUV, designated as DTMUV GDZQ2022, responsible for these effects. Comprehensive genomic sequencing of this strain was conducted to analyse its genetic variations. Additionally, the isolated and purified virus was inoculated into goose embryos and goslings to assess its pathogenicity. The GDZQ2022 genome displayed over 88% nucleotide homology with other DTMUV strains from China and Southeast Asia. Phylogenetic analysis of the E gene classified GDZQ2022 within the subgenotype 3 of DTMUV. Pathogenicity experiments on goose embryos and goslings showed that the GDZQ2022 strain induced typical clinical signs of DTMUV, particularly severe neurological manifestations. Although GDZQ2022 exhibited high virulence in goose embryos, its virulence in goslings was minimal, resulting in a low mortality rate. Pathological examinations detected significant histological lesions in the brains, livers, and spleens of the infected goslings. In conclusion, this study presents the first evidence of a novel DTMUV strain proliferating among young geese in China, underscoring the genetic diversity of DTMUV and contributing to our understanding of the pathogenicity of the subgenotype 3 Tembusu virus in goose embryos and goslings.<b>RESEARCH HIGHLIGHTS</b>Pathogenicity of subgenotype 3 duck Tembusu virus in goose embryos and goslings was shown for the first time.DTMUV GDZQ2022 strain is highly pathogenic in goose embryos.The virulence of the DTMUV GDZQ2022 strain in goslings is relatively mild.Infected goslings exhibit typical clinical manifestations, with a low mortality rate.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"175-184"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful bacteriophage therapy of pyoderma caused by methicillin-resistant Staphylococcus pseudintermedius in a dog.","authors":"Cátia Mota Sá, Cláudia Rodrigues, Vanessa Silva, Joana Azeredo, Luís D R Melo","doi":"10.1111/vde.13328","DOIUrl":"10.1111/vde.13328","url":null,"abstract":"<p><p>Pyoderma caused by methicillin-resistant Staphylococcus pseudintermedius poses significant challenges to canine health. Successful therapy requires an integrated approach to prudent antimicrobial use. This report describes the successful treatment of a dog with generalised superficial pyoderma using a topical formulation of S. pseudintermedius phage applied once daily for a period of 30 days.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"227-230"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary supplementation of rubber seed kernel pellet on feed utilization, rumen fermentation, fatty acid profiles and health status in swamp buffalo.","authors":"Nirawan Gunun, Chatchai Kaewpila, Waroon Khota, Thachawech Kimprasit, Pongsatorn Gunun","doi":"10.5713/ab.24.0354","DOIUrl":"10.5713/ab.24.0354","url":null,"abstract":"<p><strong>Objective: </strong>Rubber seed kernel is a by-product of rubber cultivation and are high in oil and protein. This study was conducted to evaluate the effect of supplementing rubber seed kernel pellet (RUSKEP) on feed intake, nutrient digestibility, rumen fermentation, rumen fatty acid profiles, blood parameters, and immune response in swamp buffalo.</p><p><strong>Methods: </strong>Four male swamp buffalo with an initial body weight (BW) of 254±10 kg and 26 months of age were used in this research. The experimental design was a 4×4 Latin squared design with RUSKEP supplementation at 0%, 4%, 6%, and 8% of dry matter intake (DMI). Animals were fed concentrate at 1.0% BW, while rice straw was fed ad libitum.</p><p><strong>Results: </strong>Supplementation with RUSKEP did not change DMI or nutrient digestibility (p>0.05), while ether extract digestibility increased cubically with the addition of RUSKEP (p<0.01). The ruminal pH, ammonia-nitrogen (NH3-N), and the proportion of acetate (C2) were similar among treatments (p>0.05). The proportion of propionate (C3) increased linearly (p≤0.04), leading to a decrease in the acetate to propionate ratio (C2:C3) (p≤0.04) with the addition of RUSKEP. Furthermore, the butyrate (C4) proportion decreased linearly with RUSKEP supplementation (p = 0.03). The addition of RUSKEP did not affect on linoleic acid (C18:2 cis-9,12+trans-9,12), or α-linolenic acid (C18:3 cis9,12,15) (p>0.05). With RUSKEP supplementation, the stearic acid (C18:0) content increased quadratically (p<0.01). The increasing level of RUSKEP was higher in cholesterol and eosinophils (p≤0.03). The immune function (IgA, IgM, and IgG) was similar among treatments (p>0.05).</p><p><strong>Conclusion: </strong>Supplementing RUSKEP with up to 8% of DMI could improve rumen fermentation efficiency without affecting feed utilization, rumen PUFA profile, or immune response in swamp buffalo.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"682-691"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Meetings, WVPA Matters and Announcements.","authors":"","doi":"10.1080/03079457.2025.2456371","DOIUrl":"https://doi.org/10.1080/03079457.2025.2456371","url":null,"abstract":"","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":"54 2","pages":"265"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical dexamethasone decelerates epithelial migration on the canine tympanic membrane.","authors":"Jihyun Kim, Taeho Oh, Seulgi Bae","doi":"10.1111/vde.13314","DOIUrl":"10.1111/vde.13314","url":null,"abstract":"<p><strong>Background: </strong>Epithelial migration (EM) is integral to normal ear structure and function. Glucocorticoids are considered the first-line therapy for various external ear disorders; however, their effects on EM on the tympanic membrane (TM) and the external auditory canal (EAC) are understudied.</p><p><strong>Hypothesis/objectives: </strong>To test the hypothesis that topical dexamethasone decelerates EM on the TM, this study aimed to evaluate the effects of topical dexamethasone on EM on the TMs of dogs.</p><p><strong>Animals: </strong>Seven ears of four healthy dogs.</p><p><strong>Materials and methods: </strong>The migration distance of an ink drop deposited on the posterior quadrant of the pars tensa (TM EM rate) of dogs was calculated over 3 weeks from images captured with a video-otoscope. The results were compared by paired Student's t-test to those obtained from a subsequent experiment in which the same dogs were administered a fresh ink drop at the same position as previously (control group), yet additionally administered 0.1% dexamethasone (0.2 mL/ear) daily for the first 14 days (treatment group).</p><p><strong>Results: </strong>One of eight ears was excluded because of an anatomical problem. The TM EM rate of the remaining seven ears decreased by 46.74% in the treatment group compared with the control group (p < 0.05).</p><p><strong>Conclusions and clinical relevance: </strong>Topical dexamethasone decelerates EM on the TM of normal dogs' ears. In turn, it is extrapolated that debris removal from the EAC may be slowed, negatively impacting the EAC environment. Consequently, when dogs with otitis receive topical dexamethasone treatment, additional treatment, such as ear canal cleaning, may be required to minimise the effects of impaired TM EM.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"159-164"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous disorders in captive psittacines, a retrospective study of 1454 cases at a university veterinary teaching hospital (1988-2021).","authors":"Stephen D White, Hugues Beaufrère, David S-M Guzman, Verena K Affolter, Lisa A Tell, Joanne Paul-Murphy, Michelle G Hawkins","doi":"10.1111/vde.13320","DOIUrl":"10.1111/vde.13320","url":null,"abstract":"<p><strong>Background: </strong>Psittacines (parrots and their allies) are kept under human care as companion animals, live exhibit specimens in zoological institutions and occasionally as research subjects. Cutaneous disorders such as feather destructive behaviour (FDB) and pododermatitis are commonly noted in clinical reviews, case reports and text book chapters.</p><p><strong>Hypothesis/objectives: </strong>To document the type, signalment associations and prevalence of cutaneous disorders in a large number of captive psittacines in an academic referral teaching hospital population.</p><p><strong>Animals: </strong>Case population at a university veterinary teaching hospital.</p><p><strong>Methods and materials: </strong>Retrospective study using computerised medical records of birds (psittacine and non-psittacine) examined between 1 January 1988 and 31 December 2021. Inclusion criteria were records of psittacines with dermatological diagnoses.</p><p><strong>Results: </strong>Of 3472 psittacines, 1454 (41.9%) had cutaneous disease. Age was strongly associated with an increased risk of cutaneous diseases. The most common cutaneous disorders were pododermatitis (n = 729) and FDB (n = 528). Female sex and increased age were associated with significantly higher odds of FDB, as were the genera Cacatua (cockatoos), Psittacus (African grey parrots), Ara (macaws) and Agapornis (lovebirds). Increased age and the genus Agapornis also were associated with significantly higher odds of pododermatitis. The most common infectious disease was mite (presumed Knemidocoptes) infestation, almost exclusively seen in budgerigars; increased age was associated with significantly lower odds of having mites.</p><p><strong>Conclusions and clinical relevance: </strong>Pododermatitis and FDB were the most common cutaneous disorders in psittacines. As 50% of psittacines with skin disease presented for nonskin concerns, clinicians should perform a dermatological examination regardless of the reason for presentation.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"205-217"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative population genomics analysis for chicken body sizes using genome-wide single nucleotide polymorphisms.","authors":"Sensen Yan, Chaoqun Gao, Kaiyuan Tian, Chengpeng Xiao, Junlai Shi, Xintao Jia, Kejun Wang, Guirong Sun, Donghua Li, Wenting Li, Xiangtao Kang","doi":"10.5713/ab.24.0347","DOIUrl":"10.5713/ab.24.0347","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the selection history, genome regions, and candidate genes associated with different chicken body sizes, thereby providing insights into the genetic basis of complex economic traits such as chicken body size and growth.</p><p><strong>Methods: </strong>In this study, a total of 217 individuals from eight breeds were selected. According to body size, they were divided into two groups: large chickens and bantam chickens, with four breeds in each group. Firstly, we investigate population structure by principal component analysis (PCA), phylogenetic tree, and ancestry component analysis. Next, we recognize runs of homozygosity (ROH) islands through calculating ROH. Finally, we carry out selection signatures analysis utilizing population differentiation index and nucleic acid diversity.</p><p><strong>Results: </strong>The population structure analysis show that large and bantam chickens are clearly separated. Large chickens are clustered together, the bantam chickens are relatively dispersed. The results of ROH island analysis show that 48 and 56 ROH islands were identified in large and bantam chickens respectively. Among the interesting ROH islands, a total of eight candidate genes were identified. In selection signatures analysis, a total of 322 selected genes were annotated in large chickens, such as POU1F1, BMP10, enrichment in 16 gene ontology (GO) terms. In bantam chickens, a total of 447 selected genes were annotated, such as IGF1, GRB10, enrichment in 20 GO terms and 2 Kyoto encyclopedia of genes and genomes pathways. The haplotype analysis results show that GRB10 has differences in chickens of different body sizes.</p><p><strong>Conclusion: </strong>By population structure, ROH islands, and selection signatures analysis, we have identified multiple genes associated with chicken body size, growth, and development (such as BMP10, IGF1, GRB10, etc). This provides a theoretical reference for the subsequent development of molecular markers for chicken body size and the analysis of the genetic mechanism of chicken body size.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"600-611"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional remodeling of gut microbiota and liver in laying hens as affected by fasting and refeeding after fasting.","authors":"Linjian Weng, Jingyi Zhang, Jianling Peng, Meng Ru, Haiping Liang, Qing Wei, Jiming Ruan, Ramlat Ali, Chao Yin, Jianzhen Huang","doi":"10.5713/ab.24.0299","DOIUrl":"10.5713/ab.24.0299","url":null,"abstract":"<p><strong>Objective: </strong>Animals will experience energy deprivation processes such as moulting, clutching, migration and long-distance transportation under natural survival conditions and in production practices, and the body will trigger a series of adaptive metabolic changes during these processes. Fasting and refeeding after fasting can induce remodeling of nutrients and energy metabolism. This study aims to investigate the mechanisms by which the gut microbiota and liver of poultry respond to energy deprivation under specific conditions.</p><p><strong>Methods: </strong>Ninety 252-day-old laying hens were randomly divided into 3 groups: (1) fed ad libitum (control group); (2) fasted from day 13 to day 17 (fasting group); (3) fasted from day 1 to day 5, then refed on a specific feeding way (refeeding group). After that, the serum, liver, jejunum tissues, and cecum contents were sampled and sent for metabolome, transcriptome, morphology, and 16S rDNA sequencing analyses, respectively.</p><p><strong>Results: </strong>Results showed that food deprivation not only observably decreased the body weight, liver index, and the villus height and villus/crypt ratio of jejunum, but also significantly changed the gut microbiota compositions, serum metabolic profiles, and the hepatic gene expression patterns of laying hens, whereas these changes were effectively reversed by the following refeeding operation. At the same time, metabolome combined transcriptome analysis revealed that both serum differential metabolites and hepatic differential expressed genes (DEGs) were consistently enriched in the lipid and amino metabolism pathways, and strong correlations were synchronously found between the differential metabolites and both of the differential gut microbial genera and DEGs, suggesting the crosstalks among gut, liver and their resulting serum metabolic products.</p><p><strong>Conclusion: </strong>The results suggested that the organism might coordinate to maintain metabolic homeostasis under energy deprivation through a combination of changes in gut microbial composition and hepatic gene expression.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"692-706"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study and subsequent functional analysis reveal regulatory mechanism underlying piglet diarrhea.","authors":"Dong Chen, Qi Shen, Rui Huang, Zhenjian Zhao, Yang Yu, Shengdi Cui, Junge Wang, Ziyang Chen, Pingxian Wu, Guoqing Tang","doi":"10.5713/ab.24.0547","DOIUrl":"10.5713/ab.24.0547","url":null,"abstract":"<p><strong>Objective: </strong>Piglet diarrhea poses a serious threat to piglet health and the livestock economy, and is one of the most pressing problems in animal husbandry. This study aims to investigate the genetic factors involved in piglet diarrhea and to identify key genes that regulate this condition.</p><p><strong>Methods: </strong>We screened 600 diarrheal piglets based on unique diarrhea scores for resequencing and conducted a genome-wide association study (GWAS). Through this process, we identified 308 single nucleotide polymorphisms (SNPs) and annotated 151 candidate genes. Extensive functional validation and systematic analysis were performed on key candidate genes KSR1, SKAP1, SLC35F6, and OR12.</p><p><strong>Results: </strong>The study found that the four key genes were involved in the regulation of piglet diarrhea through various mechanisms. OR12 affects the levels of ZO-1 and claudin-1. Changes in the expression levels of KSR1 could alter the expression of IL1-β, IL6, and TNF-α, as well as cell migration and proliferation. SKAP1 could affect the expression of CD3 and CD4, and influence the migration and proliferation ability of cells. SLC35F6 is involved in cell apoptosis through the Bcl2/BAX/caspase3 pathway and can also affect mitochondrial membrane potential.</p><p><strong>Conclusion: </strong>The results of this study provide strong support for breeding programs aimed at disease resistance and offer potential solutions to the problem of piglet diarrhea.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"612-628"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Azolla (Azolla microphylla) leaf meal and phytonutrient powder on rumen fermentation efficiency and nutrient degradability using in vitro technique.","authors":"Burarat Phesatcha, Kampanat Phesatcha, Thiwakorn Ampapon, Metha Wanapat","doi":"10.5713/ab.24.0379","DOIUrl":"10.5713/ab.24.0379","url":null,"abstract":"<p><strong>Objective: </strong>This work was to investigate the effect of using Azolla (Azolla microphylla) leaf meal and phytonutrient powder on rumen fermentation efficiency and nutrient degradability using in vitro technique.</p><p><strong>Methods: </strong>All respective treatments were imposed in a 2×4×2 Factorial arrangements according to a completely randomized design. The first factor was two ratios of roughage to concentrate (R:C at 60:40, and 40:60), the second factor was Azolla (Azolla microphylla) powder (AMP) supplementation levels (0%, 3%, 6%, and 9% of the total substrate) and the third factor was turmeric (Curcuma longa) powder (TUP) supplementation levels (0% and 2% of the total substrate).</p><p><strong>Results: </strong>Cumulative gas production at 96 h, was affected by R:C and numerically increased by AMP and TUP supplementation (p<0.05). Gas production kinetics increased with the increasing ratio of concentrate and AMP supplementation whereas TUP supplement reduced gas production. In vitro dry matter degradability was remarkably increased (p<0.05) by the R:C ratio, AMP and TUP supplementation. However, increasing R:C ratio, AMP and TUP supplementation resulted in the concentration of propionate (C3) significantly increasing (p<0.05). Acetate (C2), C2:C3 ratio, and protozoal population were improved (p<0.05), while the methane production decreased.</p><p><strong>Conclusion: </strong>Under this study, the results were obtained under the supplementation level of 9% AMP and 2% TUP of total substrate, hence, the combined use is potentially beneficial. These results revealed a potential use of AMP and TUP as a supplement to improve rumen fermentation for ruminant feeding. Nevertheless, in vivo feeding trials should be further investigated using AMP and TUP as a source of protein and phytonutrient.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":"673-681"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}