IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-06-09 DOI: 10.1080/01652176.2025.2514753

Giovanna Fusco, Alessandro Bellato, Lorena Cardillo, Agata Campione, Michela Di Roberto, Anna Cerrone, Francesca Bove, Roberta Pellicanò, Maria Ottaiano, Marco Esposito, Antonio Limone, Anna Rita Attili, Esterina De Carlo

{"title":"Large-scale evaluation of bacteriological-based method and qPCR performance for Brucellosis diagnosis in livestock using Bayesian latent class analysis.","authors":"Giovanna Fusco, Alessandro Bellato, Lorena Cardillo, Agata Campione, Michela Di Roberto, Anna Cerrone, Francesca Bove, Roberta Pellicanò, Maria Ottaiano, Marco Esposito, Antonio Limone, Anna Rita Attili, Esterina De Carlo","doi":"10.1080/01652176.2025.2514753","DOIUrl":"10.1080/01652176.2025.2514753","url":null,"abstract":"The performance of direct tests, such as bacteriological culture and qPCR, for the diagnosis of brucellosis has been evaluated in a limited number of studies, often based on small sample sizes. Moreover, the absence of a gold standard makes this assessment even more challenging. A potential alternative for evaluating the performance of direct tests is Bayesian latent class analysis (BLCA), which does not require prior knowledge of disease status or a gold standard. This study aimed to estimate the sensitivity (Se) and specificity (Sp) of bacteriological culture for brucellosis diagnosis. In a brucellosis-endemic area, a large number of seronegative and seropositive buffaloes and cattle were tested using bacteriological culture and qPCR. BLCA was applied to estimate the performance of both tests. The median Se of bacteriological culture was estimated at 61.3%, compared to 70.9% of qPCR. The median Sp was 99.6% for bacteriological culture and 89.3% for qPCR. Bacteriological culture demonstrated a higher Positive Predictive Value (PPV) than qPCR in both buffaloes and cattle, whereas the Negative Predictive Value (NPV) of the two methods did not differ significantly. These results suggest that, in settings of low brucellosis prevalence, a positive bacteriological culture has a greater predictive value than qPCR .","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-10"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets. 添加葡萄糖氧化酶作为抗生素替代品可减轻断奶仔猪腹泻，改善肠道健康。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-01 DOI: 10.1080/01652176.2024.2447592

Dan Chen, Jiaming Chen, Zemin Dong, Qingyun Cao, Hui Ye, Dingyuan Feng, Changming Zhang, Jianjun Zuo, Weiwei Wang

{"title":"Supplemental glucose oxidase as an antibiotic substitute alleviates diarrhea and improves intestinal health in weaned piglets.","authors":"Dan Chen, Jiaming Chen, Zemin Dong, Qingyun Cao, Hui Ye, Dingyuan Feng, Changming Zhang, Jianjun Zuo, Weiwei Wang","doi":"10.1080/01652176.2024.2447592","DOIUrl":"https://doi.org/10.1080/01652176.2024.2447592","url":null,"abstract":"Weaning stress-induced diarrhea is a serious issue in pig production. This study aimed to evaluate the potential of glucose oxidase (GOD) as an antibiotic substitute to alleviate diarrhea and improve gut health in weaned piglets. According to a randomized complete block design, 250 piglets weaned around 21 d of age were allocated into 5 groups (5 replicates/group), which received a basal diet without or with supplemental 200 mg/kg antibiotic, 500, 1000 and 2000 U/kg GOD, respectively. Dietary treatments did not affect (p > 0.05) growth performance of piglets. However, supplemental all doses of GOD were equivalent or superior to antibiotic to reduce (p < 0.05) diarrhea as well as increase (p < 0.05) thymus index, hepatic and colonic antioxidant properties. GOD addition at 1000 and 2000 U/kg reduced (p < 0.05) cecal and rectal pH value, respectively. They also displayed similar or superior efficacy to antibiotic in improving (p < 0.05) duodenal and jejunal morphology along with certain tight junction proteins expression of jejunum and colon. Collectively, GOD represents an antibiotic alternative to reduce diarrhea of weaned piglets through associating with ameliorations of intestinal structure and functions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples. 使用氮同位素分馏作为猪血液和毛发样本饲料转化效率的生物标志物。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI: 10.1080/10495398.2025.2473494

Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki

{"title":"The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples.","authors":"Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki","doi":"10.1080/10495398.2025.2473494","DOIUrl":"10.1080/10495398.2025.2473494","url":null,"abstract":"This study aimed to 1) Establish relationships between feed conversion efficiency (FCE; live weight gain/intake) and several biomarkers in pigs using blood and hair samples, and 2) Investigate the relative FCE performance of pigs from maternal vs. terminal genetic lines fed high vs. low energy diets. 80 male pigs (Large White x Landrace) were fed for 56 days. The terminal genetic line and pigs fed the high energy diet had 5% and 15% higher FCE than the maternal genetic line and pigs fed the low energy diet, respectively. Blood nitrogen isotopic fractionation (Δ15N; animal δ15N - feed δ15N) explained 34% more variation of FCE, compared with the blood insulin‑like growth factor‑1 (IGF‑1). The turnover rate of δ15N in plasma was faster than δ15N in blood, suggesting that blood and plasma δ15N can predict long‑term versus short‑term FCE changes. Pigs fed the high energy diets showed 13% higher live weight gain, 16% greater high standard carcass weight, and 38% higher carcass backfat than those on low‑energy diets. In conclusion, Δ15N is a more effective FCE biomarker for pigs compared to IGF‑1. Hair samples were less predictive of FCE than blood‑derived biomarkers, suggesting further refinement in the methodology of harvesting hair samples.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2473494"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between the expression levels of myogenic regulatory factor genes and carcass characteristics in Kivircik and Hungarian Merino lambs. 基维尔西克和匈牙利美利奴羔羊产肌调节因子基因表达水平与胴体性状的关系。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-23 DOI: 10.1080/10495398.2025.2479690

Korhan Arslan, Fadime Daldaban, Hulya Yalcintan, Pembe Dilara Kecici, Bekir Ozturk, Bulent Ekiz, Bilal Akyuz

{"title":"Relationship between the expression levels of myogenic regulatory factor genes and carcass characteristics in Kivircik and Hungarian Merino lambs.","authors":"Korhan Arslan, Fadime Daldaban, Hulya Yalcintan, Pembe Dilara Kecici, Bekir Ozturk, Bulent Ekiz, Bilal Akyuz","doi":"10.1080/10495398.2025.2479690","DOIUrl":"10.1080/10495398.2025.2479690","url":null,"abstract":"This study aimed to investigate the expression profiles of the myogenic regulatory genes MYOD1, MYOG, MYF5, MYF6, and MSTN in longissimus dorsi muscle, as well as the correlation of the expression levels of these genes with carcass characteristics and growth performance in the Kivircik and Hungarian Merino sheep breeds. The expression levels of the MYF5, MYF6, and MYOG genes were found to be significantly correlated with the rib proportion, the expression level of the MYOG gene was identified as being the main determinant of variations in the rib proportion in the Kivircik lambs. The regression analysis results revealed that the expression levels of the MYF5 and MSTN genes played an essential role in determining the cold carcass dressing percentage in Hungarian Merino lambs. Further, as a result of the regression analysis, the model including the expression level of the MYF6 gene demonstrated that this gene could be responsible for 36.4% of the differences observed in cold carcass weight. In conclusion, the findings of this study suggest that the expression levels of the MYF5, MYF6, and MYOG genes were associated with various carcass traits, particularly in the Kivircik breed, and these genes hold potential as markers for enhancing breed productivity.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2479690"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR testing of conjunctival swabs to detect feline leukaemia virus in domestic cats in Thailand. 泰国家猫结膜拭子检测猫白血病病毒的PCR检测。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-21 DOI: 10.1080/01652176.2025.2488025

Hsu Po Po Aung, Tawewan Issarankura Na Ayudhaya, Kridsada Chaichoun, Jarupha Taowan, Aeknarin Saechin, Supakit Buamas, Ruangrat Buddhirongawatr

{"title":"PCR testing of conjunctival swabs to detect feline leukaemia virus in domestic cats in Thailand.","authors":"Hsu Po Po Aung, Tawewan Issarankura Na Ayudhaya, Kridsada Chaichoun, Jarupha Taowan, Aeknarin Saechin, Supakit Buamas, Ruangrat Buddhirongawatr","doi":"10.1080/01652176.2025.2488025","DOIUrl":"https://doi.org/10.1080/01652176.2025.2488025","url":null,"abstract":"This study aimed to classify the Feline leukaemia virus (FeLV) infection outcomes in domestic cats in Thailand and determine the accuracy of conjunctival swabs for FeLV proviral DNA detection by comparing results to PCR testing of blood samples. Whole blood and conjunctival swabs were collected from 126 cats with and without clinical signs. Blood specimens were evaluated for p27 FeLV antigen using the SNAP Feline Immunodeficiency Virus (FIV)/FeLV Combo Test, IDEXX Laboratories. The 3'-LTR region of the proviral FeLV was amplified from both blood and conjunctival samples. The prevalence rates of progressive and regressive FeLV infections in this study were 14.3% (95% CI: 8.69-21.63) and 36.5% (95% CI: 28.12-45.55), respectively. Cats older than 12 months of age had a higher probability of being regressively infected than cats younger than 1 year (p-value = 0.039, OR =0.294, 95% CI: 0.092-0.942). Conjunctival swabs used for detecting FeLV proviral DNA demonstrated a sensitivity of 95.3% (95% CI: 86.91-99.02) and a specificity of 100% (95% CI: 94.22-100.00) compared to conventional blood samples. The observed kappa value of 0.956 indicates that conjunctival swabs are reliable and can be used as an alternative to blood venipuncture.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology of bovine leukemia virus in cattle and phylogenetic analysis for determining its prevailing genotype in Khyber Pukhtunkhwa, Pakistan. 巴基斯坦开伯尔普赫图赫瓦省牛白血病病毒的分子流行病学和确定其流行基因型的系统发育分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-17 DOI: 10.1080/10495398.2025.2486029

Farida Tahir, Umer Sadique, Farkhanda Tahir, Mikhlid H Almutairi, Abdulwahed Fahad Alrefaei, Shabana Naz, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

{"title":"Molecular epidemiology of bovine leukemia virus in cattle and phylogenetic analysis for determining its prevailing genotype in Khyber Pukhtunkhwa, Pakistan.","authors":"Farida Tahir, Umer Sadique, Farkhanda Tahir, Mikhlid H Almutairi, Abdulwahed Fahad Alrefaei, Shabana Naz, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2486029","DOIUrl":"https://doi.org/10.1080/10495398.2025.2486029","url":null,"abstract":"This research focused on assessing the molecular prevalence of Bovine Leukemia Virus (BLV) in different cattle farms throughout Khyber Pakhtunkhwa and characterizing the dominant BLV genotypes by analyzing partial sequences of the gp51 gene. A total of 1,250 blood samples were collected from cattle of both sexes, various age groups (<1 year, 1-3 years, 3-5 years, and >5 years), and different breeds (Friesian, Jersey, Sahiwal, Achai, and crossbred) from multiple cattle farms. Of the 1,250 samples tested, BLV was detected in 136 (10.88%) using nested PCR. Risk factor analysis revealed a significantly higher prevalence of BLV in exotic breeds and older cattle. To confirm the findings and genotype the BLV isolates, four PCR-positive samples were sequenced. Phylogenetic analysis identified the isolates as belonging to genotype I, closely related to GI BLV isolates from Japan. Furthermore, the isolates in this study formed a tightly clustered group, suggesting a common origin from an earlier virus introduced into the host population in the study area.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2486029"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic network of infectious bronchitis virus: exploring the impact of migratory birds on viral clustering, evolution, and recombination. 传染性支气管炎病毒的系统发育网络：探索候鸟对病毒聚集、进化和重组的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-14 DOI: 10.1080/01652176.2025.2465570

Yu-Chen Tai, Geng-Ming Hu, Chi-Ming Chen

{"title":"Phylogenetic network of infectious bronchitis virus: exploring the impact of migratory birds on viral clustering, evolution, and recombination.","authors":"Yu-Chen Tai, Geng-Ming Hu, Chi-Ming Chen","doi":"10.1080/01652176.2025.2465570","DOIUrl":"10.1080/01652176.2025.2465570","url":null,"abstract":"Infectious bronchitis virus (IBV) presents a major threat to global poultry production, necessitating a thorough understanding of its evolutionary relationships for effective control measures. This study presents a novel distance-based Minimum Span Clustering (MSClustering) method to cluster 311 IBV strains, with a comparison of its results to the established IBV classification. Phylogenetic network and recombination analyses were employed to investigate IBV evolutionary relationships and transmission pathways. The phylogenetic network revealed distinct clusters reflecting relationships between IBV strains. Importantly, these network patterns, combined with recombination event analysis, suggest an unrecognized role for migratory birds in IBV dissemination, highlighting potential transmission pathways beyond established poultry trade routes. These findings contribute to advancing our understanding of IBV evolution and support the development of targeted strategies for controlling viral outbreaks in poultry populations. While statistical limitations may affect threshold estimation for smaller networks, our MSClustering method significantly accelerates processing speeds-approximately 100,000 times faster than PhyML when analyzing the dataset-enabling comprehensive-scale phylogenetic analysis of viruses.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality and microbial community analysis of solid-state fermented feed with mixed bacteria from corn silage. 玉米青贮混合菌固态发酵饲料品质及微生物群落分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-29 DOI: 10.1080/10495398.2025.2507905

Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao

{"title":"Quality and microbial community analysis of solid-state fermented feed with mixed bacteria from corn silage.","authors":"Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao","doi":"10.1080/10495398.2025.2507905","DOIUrl":"https://doi.org/10.1080/10495398.2025.2507905","url":null,"abstract":"The efficacy of microbial fermented feeds (FF) is highly dependent on microbial composition, underscoring the need for locally sourced, cost-effective formulations to advance sustainable livestock production. In this study, bacteria from corn silage (CSB) were screened for the preparation of solid-state fermented feed (SFF). In addition, for the purpose of comparison, purchased marketed fermentation bacteria (PMB) for SFF were also tested using the same methodology. Anaerobic fermentation was carried out by adding 5% CSB, PMB, and saline to conventional feeds at 37 °C, respectively. Then 10 g of fermentation samples was collected on days 0, 3, and 5, respectively. The composition and diversity of the microbial community were subsequently analyzed using high-throughput sequencing. The pH, titratable acidity (TA), and nutrient index of the feeds before and after fermentation were determined. High-throughput sequencing results showed that the dominant genera of CSB were Weissella, Pseudomonas, and Bacteroidetes, whereas the dominant genera of PMB were Bacteroidetes, Bacillus, Pseudomonas, and Enterococcus. The dominant genera in unfermented feeds (CK) were Chlorophyta, Mitochondria, and Pseudomonas. In addition, the dominant genera in SFF supplemented with CSB and PMB were Weissella and Bacteroidetes, respectively. Compared with CK, pH of SFF supplemented with CSB and PMB decreased gradually, and TA increased significantly and then decreased gradually during fermentation. Similarly, Crude fiber content was higher than CK in both CSB and PMB, while crude fat content was lower than CK for all of them. The study illustrates that CSB can be used to ferment porcine solid feeds and that CSB solid feed microorganisms may promote intestinal absorption of complex polysaccharides in pigs, which lays a certain theoretical foundation for the application of CSB to SFF.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2507905"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplexed immunoassay for a serum autoantibody biomarker panel in diagnostic and prognostic prediction of canine mammary tumors. 犬乳腺肿瘤诊断和预后预测中血清自身抗体生物标志物的多重免疫分析。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2024-12-06 DOI: 10.1080/01652176.2024.2435978

Chih-Ching Wu, Chia-Yu Chang, Pei-Yi Chou, Xiu-Ya Chan, Chun-Chueh Huang, Youngsen Yang, Hao-Ping Liu

{"title":"Multiplexed immunoassay for a serum autoantibody biomarker panel in diagnostic and prognostic prediction of canine mammary tumors.","authors":"Chih-Ching Wu, Chia-Yu Chang, Pei-Yi Chou, Xiu-Ya Chan, Chun-Chueh Huang, Youngsen Yang, Hao-Ping Liu","doi":"10.1080/01652176.2024.2435978","DOIUrl":"10.1080/01652176.2024.2435978","url":null,"abstract":"Canine mammary tumor (CMT) is a prevalent and destructive disease often diagnosed at an advanced stage, leading to poor outcomes. Currently, there is a lack of effective biomarkers for early detection and prognostic prediction of CMT. To improve CMT detection, we established a multiplexed immunoassay using a fluorescence bead-based suspension array system to measure serum levels of autoantibodies against four CMT-associated proteins (AGR2, HAPLN1, IGFBP5, and TYMS) in CMT patients. Our data revealed that serum levels of the four autoantibodies (anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS) were significantly elevated in CMT patients (n = 158) compared to healthy individuals (n = 39). Notably, serum levels of anti-AGR2, anti-HAPLN1, and anti-TYMS in the dogs with stage I CMT (n = 56) were higher than those in the healthy group. Using a marker panel consisting of the four autoantibodies for detecting malignant CMT (n = 125) achieved a sensitivity of 50.4% and a specificity of 90%. Furthermore, higher levels of anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS were associated with poorer survival in CMT patients. Collectively, we established a multiplexed immunoassay platform to detect serum autoantibodies and demonstrated that a tailored autoantibody marker panel shows potential clinical applicability for the diagnosis and prognosis of CMT.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal^® tissue sealer in pond sliders (Trachemys scripta): impacts on procedure time, surgical access, and recovery time according to follicular stage. 传统与电刀卵巢输卵管切除术与池塘滑块（Trachemys scripta）中的EnSeal®组织密封器的比较：根据卵泡分期对手术时间、手术通路和恢复时间的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI: 10.1080/01652176.2025.2473743

Alessandro Vetere, Michela Ablondi, Martina Gavezzoli, Francesco Di Ianni

{"title":"Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal® tissue sealer in pond sliders (Trachemys scripta): impacts on procedure time, surgical access, and recovery time according to follicular stage.","authors":"Alessandro Vetere, Michela Ablondi, Martina Gavezzoli, Francesco Di Ianni","doi":"10.1080/01652176.2025.2473743","DOIUrl":"10.1080/01652176.2025.2473743","url":null,"abstract":"Pond sliders (Trachemys scripta) is a common pet and invasive species in many countries. Its ability to thrive and reproduce in Mediterranean climates necessitates population control to prevent overpopulation. Ovariectomy is considered as a preventive strategy to limit reproduction and protect local biodiversity. This study compares two spaying techniques in Trachemys scripta: electrosurgery using the EnSeal® tissue sealer (group E) and a conventional approach with manual ligation of blood vessels (group C). Statistical tests evaluated procedural differences, including ANOVA for duration (factoring in follicular stage, surgery type, and their interaction) and Chi-squared tests for the likelihood of single surgical access via the prefemoral fossa and post-surgical feeding. Pearson's Chi-squared test with Yates' correction was used to assess complications by surgery type and follicular stage. ANOVA assessed the effect of follicular stage on surgery time, with follicles classified as Class I (n = 17), Class II (n = 12), and Class III (n = 7). Both follicular stage and surgery type significantly impacted surgery time, while their interaction was not significant, indicating independent effects. The likelihood of single-access procedures was significantly linked to surgery type, with EnSeal® procedures being faster and associated with earlier post-surgical feeding. Complications were not significantly linked to surgery type or follicular stage.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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