IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-29 DOI: 10.1080/10495398.2025.2507905

Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao

{"title":"Quality and microbial community analysis of solid-state fermented feed with mixed bacteria from corn silage.","authors":"Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao","doi":"10.1080/10495398.2025.2507905","DOIUrl":"https://doi.org/10.1080/10495398.2025.2507905","url":null,"abstract":"The efficacy of microbial fermented feeds (FF) is highly dependent on microbial composition, underscoring the need for locally sourced, cost-effective formulations to advance sustainable livestock production. In this study, bacteria from corn silage (CSB) were screened for the preparation of solid-state fermented feed (SFF). In addition, for the purpose of comparison, purchased marketed fermentation bacteria (PMB) for SFF were also tested using the same methodology. Anaerobic fermentation was carried out by adding 5% CSB, PMB, and saline to conventional feeds at 37 °C, respectively. Then 10 g of fermentation samples was collected on days 0, 3, and 5, respectively. The composition and diversity of the microbial community were subsequently analyzed using high-throughput sequencing. The pH, titratable acidity (TA), and nutrient index of the feeds before and after fermentation were determined. High-throughput sequencing results showed that the dominant genera of CSB were Weissella, Pseudomonas, and Bacteroidetes, whereas the dominant genera of PMB were Bacteroidetes, Bacillus, Pseudomonas, and Enterococcus. The dominant genera in unfermented feeds (CK) were Chlorophyta, Mitochondria, and Pseudomonas. In addition, the dominant genera in SFF supplemented with CSB and PMB were Weissella and Bacteroidetes, respectively. Compared with CK, pH of SFF supplemented with CSB and PMB decreased gradually, and TA increased significantly and then decreased gradually during fermentation. Similarly, Crude fiber content was higher than CK in both CSB and PMB, while crude fat content was lower than CK for all of them. The study illustrates that CSB can be used to ferment porcine solid feeds and that CSB solid feed microorganisms may promote intestinal absorption of complex polysaccharides in pigs, which lays a certain theoretical foundation for the application of CSB to SFF.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2507905"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction statement. 撤销声明。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-03 DOI: 10.1080/10495398.2025.2528007

引用次数: 0

Multiplexed immunoassay for a serum autoantibody biomarker panel in diagnostic and prognostic prediction of canine mammary tumors. 犬乳腺肿瘤诊断和预后预测中血清自身抗体生物标志物的多重免疫分析。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2024-12-06 DOI: 10.1080/01652176.2024.2435978

Chih-Ching Wu, Chia-Yu Chang, Pei-Yi Chou, Xiu-Ya Chan, Chun-Chueh Huang, Youngsen Yang, Hao-Ping Liu

{"title":"Multiplexed immunoassay for a serum autoantibody biomarker panel in diagnostic and prognostic prediction of canine mammary tumors.","authors":"Chih-Ching Wu, Chia-Yu Chang, Pei-Yi Chou, Xiu-Ya Chan, Chun-Chueh Huang, Youngsen Yang, Hao-Ping Liu","doi":"10.1080/01652176.2024.2435978","DOIUrl":"10.1080/01652176.2024.2435978","url":null,"abstract":"Canine mammary tumor (CMT) is a prevalent and destructive disease often diagnosed at an advanced stage, leading to poor outcomes. Currently, there is a lack of effective biomarkers for early detection and prognostic prediction of CMT. To improve CMT detection, we established a multiplexed immunoassay using a fluorescence bead-based suspension array system to measure serum levels of autoantibodies against four CMT-associated proteins (AGR2, HAPLN1, IGFBP5, and TYMS) in CMT patients. Our data revealed that serum levels of the four autoantibodies (anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS) were significantly elevated in CMT patients (n = 158) compared to healthy individuals (n = 39). Notably, serum levels of anti-AGR2, anti-HAPLN1, and anti-TYMS in the dogs with stage I CMT (n = 56) were higher than those in the healthy group. Using a marker panel consisting of the four autoantibodies for detecting malignant CMT (n = 125) achieved a sensitivity of 50.4% and a specificity of 90%. Furthermore, higher levels of anti-AGR2, anti-HAPLN1, anti-IGFBP5, and anti-TYMS were associated with poorer survival in CMT patients. Collectively, we established a multiplexed immunoassay platform to detect serum autoantibodies and demonstrated that a tailored autoantibody marker panel shows potential clinical applicability for the diagnosis and prognosis of CMT.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-12"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal^® tissue sealer in pond sliders (Trachemys scripta): impacts on procedure time, surgical access, and recovery time according to follicular stage. 传统与电刀卵巢输卵管切除术与池塘滑块（Trachemys scripta）中的EnSeal®组织密封器的比较：根据卵泡分期对手术时间、手术通路和恢复时间的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI: 10.1080/01652176.2025.2473743

Alessandro Vetere, Michela Ablondi, Martina Gavezzoli, Francesco Di Ianni

{"title":"Comparison of conventional vs. electrosurgical ovariosalpingectomy with the EnSeal® tissue sealer in pond sliders (Trachemys scripta): impacts on procedure time, surgical access, and recovery time according to follicular stage.","authors":"Alessandro Vetere, Michela Ablondi, Martina Gavezzoli, Francesco Di Ianni","doi":"10.1080/01652176.2025.2473743","DOIUrl":"10.1080/01652176.2025.2473743","url":null,"abstract":"Pond sliders (Trachemys scripta) is a common pet and invasive species in many countries. Its ability to thrive and reproduce in Mediterranean climates necessitates population control to prevent overpopulation. Ovariectomy is considered as a preventive strategy to limit reproduction and protect local biodiversity. This study compares two spaying techniques in Trachemys scripta: electrosurgery using the EnSeal® tissue sealer (group E) and a conventional approach with manual ligation of blood vessels (group C). Statistical tests evaluated procedural differences, including ANOVA for duration (factoring in follicular stage, surgery type, and their interaction) and Chi-squared tests for the likelihood of single surgical access via the prefemoral fossa and post-surgical feeding. Pearson's Chi-squared test with Yates' correction was used to assess complications by surgery type and follicular stage. ANOVA assessed the effect of follicular stage on surgery time, with follicles classified as Class I (n = 17), Class II (n = 12), and Class III (n = 7). Both follicular stage and surgery type significantly impacted surgery time, while their interaction was not significant, indicating independent effects. The likelihood of single-access procedures was significantly linked to surgery type, with EnSeal® procedures being faster and associated with earlier post-surgical feeding. Complications were not significantly linked to surgery type or follicular stage.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The secretomes of bovine mammary epithelial cell subpopulations differentially modulate macrophage function. 牛乳腺上皮细胞亚群分泌组差异调节巨噬细胞功能。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-02-08 DOI: 10.1080/01652176.2025.2463338

Nikola Danev, Rebecca M Harman, Anja S Sipka, Leanne Oliveira, Lucas Huntimer, Gerlinde R Van de Walle

{"title":"The secretomes of bovine mammary epithelial cell subpopulations differentially modulate macrophage function.","authors":"Nikola Danev, Rebecca M Harman, Anja S Sipka, Leanne Oliveira, Lucas Huntimer, Gerlinde R Van de Walle","doi":"10.1080/01652176.2025.2463338","DOIUrl":"10.1080/01652176.2025.2463338","url":null,"abstract":"Bovine mammosphere-derived epithelial cell (MDEC) cultures are heterogeneous and enriched for stem and progenitor cells. We previously reported that the bovine MDEC secretome, comprised of all bioactive factors secreted by the cells, displays regenerative properties, exerts antimicrobial effects, and modulates neutrophil activity, positioning it as a promising non-antibiotic biologic therapy for infectious diseases important to the dairy industry, like mastitis. Mastitis is defined as inflammation of the udder, and it is typically caused by bacterial infection. The effect of the MDEC secretome on macrophages, a first line of defense against bacterial infections in the udder, is unknown and could impact the utility of the secretome as a therapy for mastitis. To address this, we isolated bovine monocytes from peripheral blood and maintained them as an unpolarized (M0) population or polarized them into M1 or M2 phenotypes. Macrophages cultured with the secretome of bovine MDECs were assessed for their ability to phagocytose labeled bacterial particles and accumulate reactive oxygen species (ROS). We used single-cell RNA sequencing (scRNA-seq) and fluorescence-activated cell sorting (FACS) to isolate a subpopulation of MDECs that exert enhanced effects on macrophages. We found that the secretome of MDECs that do not express cluster of differentiation (CD) 73, a cell surface enzyme used as a marker for mesenchymal stromal cells, most strongly increased macrophage phagocytosis and ROS accumulation. These findings will help optimize the generation of the bovine MDEC secretome as a suitable treatment option for mastitis.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11809179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots. 西班牙饲养场牛呼吸道疾病（BRD）感染动物中多重耐药多杀性巴氏杆菌分离株中与抗菌素耐药性相关的整合和共轭元件

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-08 DOI: 10.1080/01652176.2025.2474220

Carlos Serna, Johan Manuel Calderón Bernal, Laura Torre-Fuentes, Ángel García Muñoz, Alberto Díez Guerrier, Marta Hernández, José Francisco Fernández-Garayzábal, Ana Isabel Vela, Dolores Cid, Julio Alvarez

{"title":"Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots.","authors":"Carlos Serna, Johan Manuel Calderón Bernal, Laura Torre-Fuentes, Ángel García Muñoz, Alberto Díez Guerrier, Marta Hernández, José Francisco Fernández-Garayzábal, Ana Isabel Vela, Dolores Cid, Julio Alvarez","doi":"10.1080/01652176.2025.2474220","DOIUrl":"10.1080/01652176.2025.2474220","url":null,"abstract":"The emergence of multidrug-resistance (MDR) in Pasteurella multocida, a major contributor to bovine respiratory disease (BRD) is being increasingly reported, often linked to the carriage of antimicrobial resistance genes (ARGs) on integrative and conjugative elements (ICEs). The resistance phenotype for 19 antimicrobials was determined using broth microdilution in 75 Pasteurella multocida isolates from healthy and BRD-affected cattle from five feedlots. The genomes of 32 isolates were sequenced to identify ARG) and mobile genetic elements (MGEs) and assess their genetic diversity. MDR isolates (with phenotypic resistance to aminoglycosides, macrolides, fluoroquinolones and/or tetracyclines) were primarily found among BRD-affected compared to healthy animals. Non-susceptible isolates, belonging to ST79 and ST13, harbored point mutations and four to nine ARGs, including rarely reported mechanisms in Europe (mph(E), msr(E) and aadA31 ARGs and newly described mutations in the gyrA/parC genes). All ARGs were linked to the presence of MGEs including two ICEs, Tn7407 and the novel Tn7809, a prophage and a putative composite transposon. Clonally related isolates were found in different batches from the same feedlot, suggesting maintenance of MDR strains. Our findings demonstrate the diverse genetic basis of AMR in P. multocida from BRD-affected cattle in Spain, emphasizing the role of MGEs in the ARG dissemination.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-15"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of oral black Maca (Lepidium meyenii) supplementation on semen quality and refrigerated storage stability in subfertile and normofertile dogs: a study on sperm parameters and testosterone levels. 口服黑玛卡对低生育和正常生育犬精液质量和冷藏稳定性的影响：精子参数和睾酮水平的研究。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-05-02 DOI: 10.1080/01652176.2025.2498915

Viola Zappone, Debora Teresa Gattuso, Marco Quartuccio, Giorgia Pettina, Carmelo Cavallo, Mario Tomasello, Cristina Tomasella, Giulia Donato, Alessandro Troisi, Tiziana Caspanello

{"title":"Effects of oral black Maca (Lepidium meyenii) supplementation on semen quality and refrigerated storage stability in subfertile and normofertile dogs: a study on sperm parameters and testosterone levels.","authors":"Viola Zappone, Debora Teresa Gattuso, Marco Quartuccio, Giorgia Pettina, Carmelo Cavallo, Mario Tomasello, Cristina Tomasella, Giulia Donato, Alessandro Troisi, Tiziana Caspanello","doi":"10.1080/01652176.2025.2498915","DOIUrl":"https://doi.org/10.1080/01652176.2025.2498915","url":null,"abstract":"The aim of this study was to assess the beneficial effects of oral supplementation of Lepidium meyenii (Maca) on improving and keeping sperm quality in dogs during storage, and to investigate its effect on changes in testosterone concentrations. Forty male dogs were enrolled in the study and divided into four groups of ten dogs each: two subfertile (control and treatment) and two normofertile (control and treatment) groups. The dogs in the treatment groups received Maca in a capsule formulation (75 mg/kg), while the control groups received placebo. The spermiogram and testosterone levels were assessed at three times of the sperm cycle: 0 (T0), 31 (T31), and 62 (T62) days. Ejaculates were processed for storage at 5 °C and evaluated for total and progressive motility and membrane integrity at 3 (T3h), 24 (T24h), 48 (T48h), and 72 h (T72h) post storage. The oral supplementation of 75 mg/kg of Maca extract in dogs can improve sperm parameters and increase serum testosterone concentrations, leading to improved reproductive capacity. The semen of subjects treated with oral Maca supplementation maintained its parameters stable for a longer period when stored compared to the semen of control subjects, demonstrating the beneficial effect of the use of this extract on male fertility.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"10-22"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation. 槲皮素通过调节肝脏脂质代谢、氧化应激和炎症来缓解代谢相关的脂肪肝疾病。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2024-12-24 DOI: 10.1080/10495398.2024.2442351

Ling Jiang, Rong Yi, Huan Chen, Shuwu Wu

{"title":"Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation.","authors":"Ling Jiang, Rong Yi, Huan Chen, Shuwu Wu","doi":"10.1080/10495398.2024.2442351","DOIUrl":"https://doi.org/10.1080/10495398.2024.2442351","url":null,"abstract":"The natural flavonoid quercetin, which exhibits a range of biological activities, has been implicated in liver disease resistance in recent research. In vivo study attesting to quercetin's protective effect against metabolic-associated fatty liver disease (MAFLD) is inadequate, however. Here, our investigation explored the potential benefits of quercetin in preventing MAFLD in C57BL/6 mice fed a high-fat diet (HFD). The results revealed that quercetin ameliorated the aberrant enhancement of body and liver weight. The hepatic histological anomalie induced by MAFLD were also mitigated by quercetin. HFD-induced imbalance in serum LDL, HDL, AST, ALT, TG, and LDH was mitigated by quercetin. Mechanically, we found that quercetin improved lipid metabolism by reducing lipogenesis proteins including ACC, FASN, and SREBP-1c and enhancing β-oxidation proteins including PPARα and CPT1A. In vitro study demonstrated that quercetin regulated hepatic lipid metabolism by targeting SREBP-1c and PPARα. Additionally, quercetin enhanced the antioxidant capacity in HFD-treated mice by downregulating Nrf2 and HO-1 expressions and upregulating SOD and GPX1 expressions. The hyper-activation of inflammation was also restored by quercetin via eliminating the phosphorylation of IκBα and NF-κB p65. Collectively, our observations highlight that quercetin exerts hepatoprotective properties in MAFLD mice by regulating hepatic lipid metabolism, oxidative stress and inflammatory response.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2442351"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccine protection of broilers against various doses of wild-type Salmonella Typhimurium and changes in gut microbiota. 不同剂量野生型鼠伤寒沙门氏菌对肉鸡的疫苗保护作用及肠道菌群的变化。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2024-12-25 DOI: 10.1080/01652176.2024.2440428

Samiullah Khan, Andrea R McWhorter, Nicky-Lee Willson, Daniel M Andrews, Gregory J Underwood, Robert J Moore, Thi Thu Hao Van, Kapil K Chousalkar

{"title":"Vaccine protection of broilers against various doses of wild-type Salmonella Typhimurium and changes in gut microbiota.","authors":"Samiullah Khan, Andrea R McWhorter, Nicky-Lee Willson, Daniel M Andrews, Gregory J Underwood, Robert J Moore, Thi Thu Hao Van, Kapil K Chousalkar","doi":"10.1080/01652176.2024.2440428","DOIUrl":"https://doi.org/10.1080/01652176.2024.2440428","url":null,"abstract":"This study evaluated the impact of vaccine diluents (peptone or water) on the protective effects of Salmonella Typhimurium (S. Typhimurium) vaccine. Vaccinated broilers were challenged with different doses of wild-type S. Typhimurium through dust. At the time of cull, vaccine load was highest in caeca and lowest in spleen. Wild-type S. Typhimurium was detectable after 24 hrs only in the vaccinated birds challenged with 108 CFU and positive control. S. Typhimurium load was lower in the organs of the groups challenged with 104 and 106 compared to the 108 CFU group. The caecal microbiota alpha diversity of the vaccinated or vaccinated and challenged chickens differed from the positive and negative control groups. Beta diversity of the positive control clustered separately from all other treatment groups, showing that vaccine caused minimal changes in gut microbiota structure. The vaccinated and/or wild-type challenged chickens showed significantly higher abundance of Anaerostignum, Lachnoclostridium, Intestinimonas, Colidextribacter, Monoglobus, Acetanaerobacterium and Subdoligranulum. Outcomes from this study demonstrate that the vaccine effectively protected broiler chickens from S. Typhimurium infection and helped maintain a more stable gut microbiota structure, reducing the impact of S. Typhimurium on gut health. Vaccine diluent did not affect gut microbiota composition.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-14"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raman spectral band imaging for the diagnostics and classification of canine and feline cutaneous tumors. 拉曼光谱成像在犬猫皮肤肿瘤诊断和分类中的应用。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-09 DOI: 10.1080/01652176.2025.2486771

Mindaugas Tamošiūnas, Martynas Maciulevičius, Romans Maļiks, Diāna Dupļevska, Daira Viškere, Ilze Matīse-van Houtana, Roberts Kadiķis, Blaž Cugmas, Renaldas Raišutis

{"title":"Raman spectral band imaging for the diagnostics and classification of canine and feline cutaneous tumors.","authors":"Mindaugas Tamošiūnas, Martynas Maciulevičius, Romans Maļiks, Diāna Dupļevska, Daira Viškere, Ilze Matīse-van Houtana, Roberts Kadiķis, Blaž Cugmas, Renaldas Raišutis","doi":"10.1080/01652176.2025.2486771","DOIUrl":"10.1080/01652176.2025.2486771","url":null,"abstract":"This study introduces Raman imaging technique for diagnosing skin cancer in veterinary oncology patients (dogs and cats). Initially, Raman spectral bands (with specificity to certain molecular structures and functional groups) were identified in formalin-fixed samples of mast cell tumors and soft tissue sarcomas, obtained through routine veterinary biopsy submissions. Then, a custom-built Raman macro-imaging system featuring an intensified CCD camera (iXon Ultra 888, Andor, UK), tunable narrow-band Semrock (USA) optical filter compartment was used to map the spectral features at 1437 cm-1 and 1655 cm-1 in ex vivo tissue. This approach enabled wide-field (cm2), rapid (within seconds), and safe (< 400 mW/cm2) imaging conditions, supporting accurate diagnosis of tissue state. The findings indicate that machine learning classifiers - particularly support vector machine (SVM) and decision tree (DT) - effectively distinguished between soft tissue sarcoma, mastocytoma and benign tissues using Raman spectral band imaging data. Additionally, combining Raman macro-imaging with residual near-infrared (NIR) autofluorescence as a bimodal imaging technique enhanced diagnostic performance, reaching 85 - 95% in accuracy, sensitivity, specificity, and precision - even with a single spectral band (1437 cm-1 or 1655 cm-1). In conclusion, the proposed bi-modal imaging is a pioneering method for veterinary oncology science, offering to improve the diagnostic accuracy of malignant tumors.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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