IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-23 DOI: 10.1080/10495398.2025.2480176

Hailan Ma, Dacheng Liu, Chen Song, Hongliang Fan, Weiguang Zhou, Hongxia Zhao

{"title":"Cefoxitin inhibits the formation of biofilm involved in antimicrobial resistance MDR Escherichia coli.","authors":"Hailan Ma, Dacheng Liu, Chen Song, Hongliang Fan, Weiguang Zhou, Hongxia Zhao","doi":"10.1080/10495398.2025.2480176","DOIUrl":"10.1080/10495398.2025.2480176","url":null,"abstract":"The study investigates the relationship between biofilm formation and antibiotic resistance in Escherichia coli (E. coli) isolated from calves. Using biochemical and molecular methods, we identified the isolates and assessed their biofilm-forming ability through an improved crystal violet staining method. The minimum inhibitory concentrations (MICs) of 18 antibiotics against the isolates were determined using the broth microdilution method. The impact of cefoxitin on biofilm formation was analyzed using laser scanning confocal microscopy (LSCM). Additionally, qRT-PCR was employed to evaluate the expression levels of biofilm-related genes (luxS, motA, fliA, pfs, and csgD) in response to varying cefoxitin concentrations. Results indicated a significant correlation between antimicrobial resistance (AMR) and biofilm formation ability. Cefoxitin effectively reduced biofilm formation of multidrug-resistant E. coli isolates at 1/2 and 1 MIC, with enhanced inhibition at higher concentrations. The QS-related genes luxS, pfs, motA, and fliA were downregulated, leading to decreased csgD expression. At 1/2 MIC, csgD expression was significantly reduced. In conclusion, cefoxitin inhibits biofilm formation in multidrug-resistant E. coli by down-regulating key genes, offering a potential strategy to mitigate resistance and control infections in calves caused by biofilm-positive E. coli isolates.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2480176"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the interplay between Eimeria spp. infection and the host: understanding the dynamics of gut barrier function. 探索艾美耳球虫感染与宿主之间的相互作用：了解肠道屏障功能的动态。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-20 DOI: 10.1080/01652176.2025.2452169

Pan Chen, Mujeeb Ur Rehman, Yanfeng He, Aoyun Li, Fuchun Jian, Longxian Zhang, Shucheng Huang

{"title":"Exploring the interplay between Eimeria spp. infection and the host: understanding the dynamics of gut barrier function.","authors":"Pan Chen, Mujeeb Ur Rehman, Yanfeng He, Aoyun Li, Fuchun Jian, Longxian Zhang, Shucheng Huang","doi":"10.1080/01652176.2025.2452169","DOIUrl":"10.1080/01652176.2025.2452169","url":null,"abstract":"Coccidiosis is a global disease caused by protozoans, typically including Eimeria spp., which pose a significant threat to the normal growth and development of young animals. Coccidiosis affects mainly the gut, where parasite proliferation occurs. The intestinal barrier, which consists of chemical, mechanical, biological, and immune defences, plays a crucial role in protecting the host against pathogens, xenobiotics, and toxins present in the gastrointestinal tract. When animals ingest sporulated Eimeria spp. oocysts, these parasites primarily reproduce in the intestinal tract, causing damage to the structure and function of the intestine. This disruption of intestinal homeostasis adversely affects animal health. Numerous studies have also revealed that Eimeria-infected animals experience slower bone growth rates, inferior meat quality, reduced egg production and quality, as well as impaired growth and development. Therefore, the purpose of this review is to examine the underlying mechanisms through which Eimeria spp. regulate intestinal damage and disturb the balance of the internal environment. Specifically, this review will focus on their effects on the structural basis of the host intestine's chemical, mechanical, biological and immune barriers. This understanding is crucial for the development of effective drugs to prevent the invasion of Eimeria spp. into the intestine, which is of paramount importance for maintaining host health.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-22"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical evaluation of HuDo-CSPG4 DNA electroporation as adjuvant treatment for canine oral malignant melanoma: comparison of two vaccination protocols. HuDo-CSPG4 DNA电穿孔辅助治疗犬口腔恶性黑色素瘤的临床评价：两种疫苗接种方案的比较

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/01652176.2025.2473717

Mariateresa Camerino, Davide Giacobino, Lidia Tarone, Alfredo Dentini, Marina Martano, Emanuela Morello, Erica Ilaria Ferraris, Luca Manassero, Selina Iussich, Lorella Maniscalco, Federica Cavallo, Federica Riccardo, Paolo Buracco

{"title":"Clinical evaluation of HuDo-CSPG4 DNA electroporation as adjuvant treatment for canine oral malignant melanoma: comparison of two vaccination protocols.","authors":"Mariateresa Camerino, Davide Giacobino, Lidia Tarone, Alfredo Dentini, Marina Martano, Emanuela Morello, Erica Ilaria Ferraris, Luca Manassero, Selina Iussich, Lorella Maniscalco, Federica Cavallo, Federica Riccardo, Paolo Buracco","doi":"10.1080/01652176.2025.2473717","DOIUrl":"10.1080/01652176.2025.2473717","url":null,"abstract":"Canine oral malignant melanoma (OMM) is an aggressive, spontaneously occurring tumor carrying a poor to guarded prognosis and relatively limited therapeutic strategies. In this landscape, chondroitin sulfate proteoglycan (CSPG)4 represents a promising immunotherapeutic target. The objective of this bi-center prospective study was to examine the clinical outcome of OMM-bearing dogs treated with surgery and adjuvant electroporation using a DNA vaccine (HuDo-CSPG4) encoding both human (Hu) and canine (Do) portions of CSPG4 through two different vaccination protocols. Dogs with stage I-III surgically resected CSPG4-positive OMM underwent HuDo-CSPG4 plasmid electroporation starting at the 3rd-4th post-operative week; electrovaccination was repeated after 2 weeks. In protocol 1, electrovaccination was then delivered monthly while in protocol 2, electrovaccination was performed monthly four additional times followed by semestral boosters. The survival rates of HuDo-CSPG4-vaccinated dogs were estimated and compared with a control group treated with surgery alone. Significantly longer overall survival times were observed in HuDo-CSPG4 vaccinated dogs as compared with non-vaccinated controls. Dogs receiving protocol 2 showed similar outcomes to those of dogs undergoing protocol 1, despite fewer vaccinations. The comparable humoral response against CSPG4 resulting from the administration of protocol 1 and 2 appears to have similar clinical relevance, highlighting protocol 2 as the optimal vaccination schedule.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-16"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomic analysis reveals the emergence and dissemination of different Salmonella enterica serovar Gallinarum biovar Gallinarum lineages in Brazil. 比较基因组分析揭示了不同肠炎沙门氏菌在巴西的出现和传播。

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-02-13 DOI: 10.1080/03079457.2025.2458601

Diéssy Kipper, Renato Hohl Orsi, Nathalie de Souza Zanetti, Silvia De Carli, Andrea Karoline Mascitti, André Salvador Kazantzi Fonseca, Nilo Ikuta, Martin Wiedmann, Vagner Ricardo Lunge

引用次数: 0

Efficient differentiation between Salmonella Pullorum and Salmonella Gallinarum by a fimH-based PCR-HRM. 基于荧光定量PCR-HRM的白痢沙门氏菌与鸡痢沙门氏菌的高效鉴别

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-01-21 DOI: 10.1080/03079457.2025.2450840

Di Zhang, Linlin Zhuang, Yi Jiang, Yi Yang, Ming Xu, Xinhong Dou, Jiansen Gong

{"title":"Efficient differentiation between Salmonella Pullorum and Salmonella Gallinarum by a fimH-based PCR-HRM.","authors":"Di Zhang, Linlin Zhuang, Yi Jiang, Yi Yang, Ming Xu, Xinhong Dou, Jiansen Gong","doi":"10.1080/03079457.2025.2450840","DOIUrl":"10.1080/03079457.2025.2450840","url":null,"abstract":"Salmonella Pullorum (S. Pullorum) and Salmonella Gallinarum (S. Gallinarum) are the biovars of Salmonella enterica serovar Gallinarum that are responsible for pullorum disease and fowl typhoid, respectively, in poultry. Traditional serological methods fail to quickly differentiate between these biovars due to their identical O antigenic factors (O9 and O12). Although single nucleotide polymorphism (SNP)-based methods have been used to distinguish between the biovars, they often lack the required accuracy and effectiveness. In this study, we developed a PCR high resolution melt (PCR-HRM) assay, which targeted a SNP at position 665 of the fimH gene, for rapid differentiation between S. Pullorum and S. Gallinarum. Our method showed 100% specificity and was able to detect as little as 0.033 pg of S. Pullorum DNA and 0.027 pg of S. Gallinarum DNA. The PCR-HRM results for 547 clinical isolates were in complete agreement with traditional serological methods. This PCR-HRM assay significantly reduced identification time and provided high throughput, efficient testing. This makes it a practical and reliable tool for accurate differentiation between S. Pullorum and S. Gallinarum in clinical settings.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"450-454"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and efficacy of an inactivated tetravalent water-in-oil emulsion Escherichia coli vaccine against the E. coli peritonitis syndrome. 抗大肠杆菌腹膜炎综合征的四价油包水乳剂灭活大肠杆菌疫苗的安全性和有效性

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-01-17 DOI: 10.1080/03079457.2024.2448510

W J M Landman, J H H van Eck, M Schellekens, A Feberwee

引用次数: 0

Co-infections of Enterococcus cecorum and various avian pathogens resulted in varying rates of SPF broilers with an E. cecorum infection. 盲肠球菌与多种禽类病原体的共同感染导致SPF级肉鸡盲肠球菌感染的发生率不同。

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-01-29 DOI: 10.1080/03079457.2025.2450428

Thijs Manders, Merlijn Kense, Remco Dijkman, Jeanine Wiegel, Mieke Matthijs, Sjaak de Wit

引用次数: 0

Comparison of the efficacy of Mycoplasma gallisepticum vaccine programmes in chickens. 胆囊支原体疫苗在鸡中的效力比较。

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-05-27 DOI: 10.1080/03079457.2024.2443508

Naola Ferguson-Noel, Marianne Dos Santos, Mohammadreza Ehsan, Eniope B Oluwayinka

引用次数: 0

Histomorphological characteristics of keel bone maturation and keel bone fractures in laying hens. 蛋鸡龙骨成熟和龙骨骨折的组织形态学特征。

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-02-05 DOI: 10.1080/03079457.2024.2448498

Páll Gretarsson, Käthe Kittelsen, Randi Oppermann Moe, Ingrid Toftaker, Ida Thøfner

{"title":"Histomorphological characteristics of keel bone maturation and keel bone fractures in laying hens.","authors":"Páll Gretarsson, Käthe Kittelsen, Randi Oppermann Moe, Ingrid Toftaker, Ida Thøfner","doi":"10.1080/03079457.2024.2448498","DOIUrl":"10.1080/03079457.2024.2448498","url":null,"abstract":"The keel bone in laying hens is prone to fractures, especially on the caudal third of the keel, which is also the last part to ossify. Keel bone fractures (KBF) typically occur between 25 and 50 weeks of age (WOA). However, the keel is fully ossified at around 40 WOA, suggesting fractures can occur before ossification is complete. To better understand the relationship between KBF and ossification, this descriptive study examined keel bone morphology during maturation. Keel bones from 50 commercial aviary housed Dekalb White laying hens were collected at 10 timepoints from 17-53 WOA and prepared for histological analysis. The samples were stained with haematoxylin and eosin and Safranin O to show cartilage, ossification, bone tissue, and KBF. The results indicated an ossification process similar to endochondral ossification. The degree of ossification varied between individuals of the same age. The age at complete ossification varied from 28-49 WOA. None of the keels from hens aged 53 WOA were fully ossified. Cartilage canals were present in the keel cartilage. Medullary bone was observed in all age groups. Most fractures lacked tissue morphologies typical of high-impact collisions, suggesting the need for further research into the underlying causes. This is the first study to detail keel bone histomorphology in commercial laying hens, providing baseline data for future research.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"411-426"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and structural factors contributing to the dominance and persistence of goose astrovirus type 2. 鹅星状病毒2型显性和持久性的遗传和结构因素。

IF 2.5 2区农林科学

Avian Pathology Pub Date : 2025-08-01 Epub Date: 2025-02-05 DOI: 10.1080/03079457.2025.2454416

Peng Jiang, Aolin Li, Hongfu Ma, Yankun Lan, Jiaguo Wang, Xin Wang, Liang Zhao, Qifei Wu, Yong Wang, Xu Guo

引用次数: 0

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