Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance.

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-13 DOI:10.1080/10495398.2025.2463995
Ioannis Brouklogiannis, Konstantinos C Mountzouris
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引用次数: 0

Abstract

The study assessed the inclusion level effects of a phytogenic blend (PB) on performance and critical molecular biomarkers related to detoxification (Aryl hydrocarbon receptor; AhR) and antioxidant (Nuclear factor-erythroid 2-related factor 2; Nrf2) responses in layers' ovary and liver. Layers (n = 385; 21-week-old; Hy-Line Brown) were allotted to 5 treatments with 7 replicates of 11 hens each, for a 12-week feeding trial. Treatments were: control (CON) without PB or supplementation with PB at 250 (PB250), 750 (PB750), 1000 (PB1000) and 1500 mg/kg diet (PB1500), respectively. Performance was determined weekly for the 12-week experimental period. At the 6th and 12th experimental week ovarian and liver samples were collected for gene expression analysis. Increasing PB inclusion level, improved linearly and quadratically overall laying rate, egg mass and FCR, with optimal (P ≤ 0.001) performance notable in the PB750 compared to CON. The nutrigenomic analysis revealed that PB inclusion resulted (P ≤ 0.05) in consistent beneficial modulation of the AhR/Nrf2 pathway-related genes assessed at the 6th and 12th experimental week, both in the ovary and the liver of laying hens. In conclusion, PB beneficially modulated the ovarian and hepatic adaptive cytoprotection and supported the laying performance improvements recorded, with PB750 displaying the optimal benefits.

卵巢和肝脏植物性细胞保护功能的营养基因组学证据为提高蛋鸡生产性能提供了机制支持。
该研究评估了植物性混合物(PB)的包合水平对性能和与解毒相关的关键分子生物标志物(芳烃受体;AhR)和抗氧化剂(核因子-红细胞2相关因子2;Nrf2)在蛋鸡卵巢和肝脏中的反应。图层(n = 385;21-week-old;选取海兰褐鸡,随机分为5个处理,每处理7个重复,每个重复11只鸡,进行为期12周的饲养试验。试验组为对照组(CON),不添加PB或添加PB,分别为250 (PB250)、750 (PB750)、1000 (PB1000)和1500 mg/kg饲粮(PB1500)。在12周的试验期,每周测定一次生产性能。在实验第6周和第12周采集卵巢和肝脏样本进行基因表达分析。提高PB包埋水平,可提高总产蛋率、产蛋量和产蛋率,且PB750的产蛋性能较con最佳(P≤0.001)。营养基因组学分析显示,在试验第6周和第12周,PB包埋对蛋鸡卵巢和肝脏中AhR/Nrf2通路相关基因的调节作用一致(P≤0.05)。综上所述,PB有利于调节卵巢和肝脏的适应性细胞保护,支持所记录的蛋鸡产蛋性能的提高,其中PB750的效果最佳。
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来源期刊
Animal Biotechnology
Animal Biotechnology 工程技术-奶制品与动物科学
CiteScore
2.90
自引率
5.40%
发文量
230
审稿时长
>12 weeks
期刊介绍: Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes
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