Diseases of aquatic organisms最新文献

Histological progression and bacterial load dynamics of Renibacterium salmoninarum in Chinook salmon Oncorhynchus tshawytscha. 奇努克鲑鱼体内沙门氏菌的组织学进展和细菌负荷动态。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-05-22 DOI: 10.3354/dao03852

Riley E Dils, Tawni B R Firestone, Paula A Schaffer, Dana L Winkelman, Eric R Fetherman

{"title":"Histological progression and bacterial load dynamics of Renibacterium salmoninarum in Chinook salmon Oncorhynchus tshawytscha.","authors":"Riley E Dils, Tawni B R Firestone, Paula A Schaffer, Dana L Winkelman, Eric R Fetherman","doi":"10.3354/dao03852","DOIUrl":"https://doi.org/10.3354/dao03852","url":null,"abstract":"Renibacterium salmoninarum, the cause of bacterial kidney disease (BKD), severely impacts salmonid populations. Much of our understanding of the BKD pathology in salmonids comes from evaluating fatal infections in wild populations or spawning Pacific Northwest salmonids. Our study investigated the histological progression and bacterial load dynamics of R. salmoninarum infection in Chinook salmon following intraperitoneal injection to enhance understanding of the disease's dynamics. Seventy presmolt salmon were injected with the ATCC-33209 isolate of R. salmoninarum and monitored over 10 wk. Histological signs of disease were observed in spleen and liver tissues at 1 wk post-injection and in kidney tissues at 4 wk post-injection, with signs of disease increasing over time. Additionally, histopathological analysis revealed splenic and hepatic capsulitis (coelomitis), individual hepatocyte necrosis, progressive granulomatous hepatitis, splenitis, and nephritis with necrosis. Gram staining confirmed the presence of Gram-positive bacteria within macrophages and extracellularly in infected tissues. Quantitative PCR revealed significant increases in bacterial loads in both kidney and liver tissues over the study period, with higher bacterial loads observed in the kidney tissue. Bacterial load was strongly correlated with disease stage, with peak bacterial burdens coinciding with the most severe histological changes. Our study provides a comprehensive account of R. salmoninarum infection and disease progression in Chinook salmon after intraperitoneal injection.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"85-97"},"PeriodicalIF":1.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary silver nanoparticle effects on Penaeus vannamei growth, histopathology, faecal microbiome, and acute hepatopancreatic necrosis disease survival. 饲料纳米银颗粒对凡纳滨对虾生长、组织病理学、粪便微生物组和急性肝胰腺坏死病生存的影响

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-05-08 DOI: 10.3354/dao03848

Maribel Maldonado-Muñiz, M G Nieto-López, Mireya Tapia-Salazar, Bruno Gómez-Gil, A Guerrero, Rodolfo Lozano-Olvera, Lucia Elizabeth Cruz-Suarez, Sonia A Soto-Rodriguez

{"title":"Dietary silver nanoparticle effects on Penaeus vannamei growth, histopathology, faecal microbiome, and acute hepatopancreatic necrosis disease survival.","authors":"Maribel Maldonado-Muñiz, M G Nieto-López, Mireya Tapia-Salazar, Bruno Gómez-Gil, A Guerrero, Rodolfo Lozano-Olvera, Lucia Elizabeth Cruz-Suarez, Sonia A Soto-Rodriguez","doi":"10.3354/dao03848","DOIUrl":"https://doi.org/10.3354/dao03848","url":null,"abstract":"This study examines acute hepatopancreatic necrosis disease (AHPND) in shrimp farming, caused by specific strains of Vibrio (Vp AHPND+), and evaluates the potential of silver and silver chloride nanoparticles (Ag/AgCl NP) synthesised from marine resources as an antibacterial additive in shrimp feed. Penaeus vannamei juveniles were fed diets supplemented with 0, 10, 100, and 1000 mg Ag/AgCl per kg for 20 d. The highest weight gain (%WG) was observed in individuals consuming the 100 mg Ag kg-1 diet, with a 104.27 ± 4.11% increase. Histopathological analysis revealed that 10 and 100 mg Ag kg-1 improved hepatopancreas (HP) ultrastructure, whereas 1000 mg Ag kg-1 caused severe lesions, including cell necrosis. After infection with Vp AHPND+, survival rates increased from 27% in the control group to 78% in juveniles fed the 100 mg Ag kg-1 diet for 7 d. Microbiome analysis showed no significant changes in alpha diversity due to Ag/AgCl NP doses, although a notable shift was observed during the depuration phase. These findings highlight the potential of Ag/AgCl NP as a dietary supplement to improve growth, health, and disease resistance in shrimp farming, emphasising the importance of precise dosing to maximize benefits while minimising risks.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"35-49"},"PeriodicalIF":1.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic detection and discrimination of closely related oyster pathogens for biosecurity and shellfish stock management. 近缘牡蛎病原菌的遗传检测与鉴定及其在生物安全和贝类种群管理中的应用。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-05-08 DOI: 10.3354/dao03850

Jaret P Bilewitch, Henry S Lane, Kathryn H Wiltshire, Marty R Deveney, Amber R Brooks, Keith P Michael

{"title":"Genetic detection and discrimination of closely related oyster pathogens for biosecurity and shellfish stock management.","authors":"Jaret P Bilewitch, Henry S Lane, Kathryn H Wiltshire, Marty R Deveney, Amber R Brooks, Keith P Michael","doi":"10.3354/dao03850","DOIUrl":"https://doi.org/10.3354/dao03850","url":null,"abstract":"Disease outbreaks among shellfish cause significant declines in both wild fisheries and aquaculture production globally. In Aotearoa New Zealand, a long history of flat oyster infections by the haplosporidian parasite Bonamia exitiosa has influenced fishery dynamics in the Foveaux Strait. B. ostreae, which causes high host mortality, was detected in both wild and farmed oysters in the Marlborough Sounds in 2015 and at Stewart Island in 2017, prompting the development of rapid diagnostic tests with high sensitivity and specificity that can be used to simultaneously monitor both Bonamia species. Separate molecular diagnostic assays were developed for B. exitiosa and B. ostreae using droplet digital PCR (ddPCR). Analytical tests indicated both assays were specific and had limits of detection below 0.4 copies µl-1. Estimates of diagnostic performance used latent class analysis conducted on comparisons with previously established test methods. Both ddPCR assays had a diagnostic sensitivity and specificity equal to or greater than tests based on qPCR, end-point PCR, histopathology and heart imprints, validating their use for fishery health assessment and biosecurity monitoring. The new ddPCR assays are preferred over other methods in situations where high throughput and analytic and diagnostic performance are essential, as seen in New Zealand where 2 Bonamia species are sympatric in some regions and may co-infect a single host. Furthermore, the new assays could be adapted for eDNA-based surveillance and combined with other pathogen assays for multiplex assays, enabling further research into Bonamia lifecycles.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"51-70"},"PeriodicalIF":1.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of three point-of-care blood testing instruments for rapid on-site health monitoring of Atlantic salmon Salmo salar. 三种点护理血液检测仪器在大西洋鲑现场快速健康监测中的比较。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-05-08 DOI: 10.3354/dao03851

Saad Zah, Eric Bendiksen, Ioannis Vatsos, André Madsen, Kjetil Korsnes

{"title":"Comparison of three point-of-care blood testing instruments for rapid on-site health monitoring of Atlantic salmon Salmo salar.","authors":"Saad Zah, Eric Bendiksen, Ioannis Vatsos, André Madsen, Kjetil Korsnes","doi":"10.3354/dao03851","DOIUrl":"https://doi.org/10.3354/dao03851","url":null,"abstract":"Biomarkers in blood are useful for assessing health and welfare in animals. This study evaluated the agreement among 3 point-of-care testing (POCT) instruments (Seamaty SMT-120VP, Mnchip Pointcare V2/V3, and Zoetis Vetscan VS2 analyzer) on Atlantic salmon Salmo salar. A repeatability study investigated internal measurement variation. In total, 60 plasma samples from adult fish were analyzed simultaneously using different rotors with multiple biomarkers. A comparison between blood and plasma was conducted on 35 blood samples. Lin's concordance correlation coefficient was <0.9 for all analyte comparisons between the 3 POCT except for bile acids; therefore, the McBride strength of agreement was generally poor and was moderate for bile acids. Internal measurement showed a low coefficient of variation for most analytes, except for aspartate aminotransferase (Pointcare V2/V3), alanine transaminase (Pointcare V2/V3), blood urea nitrogen (Pointcare V2/V3), and creatinine (Pointcare V2/V3, SMT-120VP). There was high concordance between whole blood and plasma samples for most analytes on both SMT-120VP and Pointcare V2/V3 systems, except for sodium, total bilirubin, and total CO2. This study underscores the necessity for system-specific calibration and validation of POCT systems like the Seamaty SMT-120VP and Mnchip Pointcare V2/V3 when used in aquaculture for clinical assessment of Atlantic salmon. The reproducibility study demonstrated that the precision of analysis was acceptable for most analytes. The comparison between whole blood and plasma suggests that whole blood can be used on-site to reduce the complexity of analysis. In summary, these systems offer promising tools for rapid on-site health monitoring in salmonid aquaculture but they require validation against gold-standard methods.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"71-83"},"PeriodicalIF":1.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutrient enrichment alters the microbiome and increases chytrid load in the American bullfrog Lithobates catesbeianus. 营养物质的富集改变了美洲牛蛙体内的微生物群，增加了壶菌负荷。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-04-24 DOI: 10.3354/dao03846

Katie Pincus, Patrick J Kearns, Kaitlyn Williams, Douglas C Woodhams

{"title":"Nutrient enrichment alters the microbiome and increases chytrid load in the American bullfrog Lithobates catesbeianus.","authors":"Katie Pincus, Patrick J Kearns, Kaitlyn Williams, Douglas C Woodhams","doi":"10.3354/dao03846","DOIUrl":"https://doi.org/10.3354/dao03846","url":null,"abstract":"Agricultural practices have a profound impact on watershed dynamics, water quality, and the well-being of aquatic life. One major concern is agricultural pollution, particularly the excess of nutrients, which can elevate disease risks in various host-pathogen relationships. However, the exact mechanisms driving this effect remain uncertain. Elevated nutrient levels are believed to significantly influence populations of aquatic environmental bacteria, potentially reshaping the microbiomes of aquatic organisms and affecting their vulnerability to disease. Despite this, the impact of nutrient enrichment on host microbiomes as a link to diseases in aquatic organisms has been largely overlooked. In this study, we investigated the impact of nutrient enrichment on the skin-associated microbial communities of the American bullfrog Lithobates catesbeianus. We observed a significant shift in bacterial richness and community composition in nutrient-enriched ponds compared with reference ponds. Although the proportion of the community inhibitory towards Batrachochytrium dendrobatidis (Bd) did not change significantly, Bd loads were markedly higher in nutrient-enriched ponds. Nutrient enrichment significantly altered carbon utilization patterns as measured by Biolog EcoPlates, and antibiotic resistance was prevalent across all ponds and samples, with resistance to trimethoprim, sulfamethazine, and chloramphenicol significantly higher in nutrient-enriched ponds. Our findings indicate that nutrient enrichment affects the structure and function of skin-associated microbial communities in American bullfrogs, influencing both Bd load and antibiotic resistance.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"27-34"},"PeriodicalIF":1.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lateral flow nucleic acid assay for Ecytonucleospora hepatopenaei based on recombinase polymerase amplification and strand displacement reaction. 基于重组酶聚合酶扩增和链置换反应的肝芽胞核孢子虫侧流核酸检测。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-04-17 DOI: 10.3354/dao03847

Na Ying, Yuan Wang, Bo Qin, Yanqing Wu, Zitong Wang, Huijuan Chen, Xuefeng Song, Zhixing Su, Wenhong Fang

{"title":"Lateral flow nucleic acid assay for Ecytonucleospora hepatopenaei based on recombinase polymerase amplification and strand displacement reaction.","authors":"Na Ying, Yuan Wang, Bo Qin, Yanqing Wu, Zitong Wang, Huijuan Chen, Xuefeng Song, Zhixing Su, Wenhong Fang","doi":"10.3354/dao03847","DOIUrl":"https://doi.org/10.3354/dao03847","url":null,"abstract":"The incidence of Ecytonucleospora hepatopenaei (EHP) infections in farmed shrimp has increased markedly in recent years, resulting in significant economic losses for the global shrimp farming industry. The lack of an efficacious drug for EHP infection has led to the development of a strategy based on the timely screening and elimination of EHP-carrying shrimp seeds as a means of preventing financial loss. This strategy requires portable, accurate and rapid detection methods for EHP, especially when applied to sites such as farms. However, the current lack of user-friendly devices capable of real-time detection under field conditions represents a significant challenge in the implementation of this strategy. In this study, an isothermal amplification nucleic acid biosensor for EHP detection was developed. The biosensor targeted the spore wall protein gene of EHP and amplified the target gene by recombinase polymerase amplification (RPA) combined with strand displacement reaction (SDR). The amplified products were applied on gold nanoparticle-based lateral flow nucleic acid strips (LFNAS) for visual signal conversion. The limit of detection of the SDR-RPA-LFNAS assay was 7 copies reaction-1, and the entire process could be completed in 30 min without cross-reaction. In contrast to existing conventional RPA-related detection methods, the introduction of SDR, which is used to eliminate the background signal produced by long primers, avoids the use of endonucleases and reduces costs. Moreover, the biosensor is straightforward to operate and does not require the use of expensive machinery, rendering it more suitable for the in situ detection of EHP in shrimp farms or aquaculture facilities.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"17-26"},"PeriodicalIF":1.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of native and invasive non-native marine invertebrate species as carriers for pathogens Vibrio spp. and ostreid herpesvirus-1 µVar. 原生和入侵的非原生海洋无脊椎动物作为病原菌弧菌和ostreid herpesvirus-1µVar载体的作用

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-04-04 DOI: 10.3354/dao03844

Hollie Soppitt, Cillian Meehan, Sarah C Culloty, Sharon A Lynch

{"title":"Role of native and invasive non-native marine invertebrate species as carriers for pathogens Vibrio spp. and ostreid herpesvirus-1 µVar.","authors":"Hollie Soppitt, Cillian Meehan, Sarah C Culloty, Sharon A Lynch","doi":"10.3354/dao03844","DOIUrl":"10.3354/dao03844","url":null,"abstract":"Invasive non-native species (INNS) are expanding their geographic range due to climate change, maritime traffic (primary route) and aquaculture (secondary route), resulting in the potential spread of microbes associated with them. Few studies have investigated the INNS-pathogen phenomenon. In this study, marine invertebrate species (native and INNS) were sampled monthly over 3 mo and screened by PCR for the ostreid herpesvirus-1 microvariant (OsHV-1 μVar) and Vibrio bacteria. Both pathogens are negatively associated with bivalve aquaculture. Sample sites included a shipping port, an oyster farm, a marsh nature reserve and a riverine site. Crustacea, Mollusca, Polychaeta, Tunicata and Porifera were sampled. Vibrio spp. were detected in 54.3% (n = 319/588) across all taxa and sample sites. The first detection of V. salmonicida associated with Atlantic salmon Salmo salar was detected in the INNS beaked barnacle Austrominius modestus. OsHV-1 μVar (7.7%, 45/588) was detected in Crustacea, Mollusca and Polychaeta at non-culture sites and in mussels Mytilus spp. at a much lower temperature (average sea surface temperature, SST, 11.25°C) than previously recorded. The shipping port had the highest Vibrio diversity and OsHV-1 μVar detection. Over half (51.1%) of 'recently dead' shore crabs Carcinus maenas had either pathogen detected compared to 29.4% of living crabs. OsHV-1 μVar detection was significantly higher in dead crabs (24.4%) compared to living crabs (5.9%). Findings from this study contribute a better understanding of the role of estuarine native and INNS as vectors/carriers of pathogens and of how the spread of INNS might facilitate the spread of pathogens.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"1-15"},"PeriodicalIF":1.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-lethal detection of the invasive American eel parasite Anguillicola crassus. 入侵美洲鳗鲡寄生虫的非致死检测。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-03-20 DOI: 10.3354/dao03849

Danielle R Lavoie, Kenneth Oliveira

{"title":"Non-lethal detection of the invasive American eel parasite Anguillicola crassus.","authors":"Danielle R Lavoie, Kenneth Oliveira","doi":"10.3354/dao03849","DOIUrl":"10.3354/dao03849","url":null,"abstract":"The invasive species Anguillicola crassus is a nematode that infects the swimbladders of anguillid eels. Heavy, repeated infections cause the swimbladder to become thickened and scarred, which can alter swimbladder gas volume, increase energy demands of buoyancy regulation, and influence normal function. Silver-phase (sexually maturing) eels migrate up to thousands of kilometers to the Sargasso Sea to reproduce, and increased energetic requirements may be detrimental to migration and breeding success. Currently, the best practice to confirm A. crassus infection is to dissect an eel and examine the swimbladder. We used a portable digital X-ray system to determine the presence of A. crassus in American eels Anguilla rostrata. Silver-phase eels were anesthetized and radiographed. Post-imaging, individuals were dissected to compare the contents of the swimbladder to the radiographs. Infections appeared opaque on radiographs. Results showed no false positives and an accuracy of 74.8%. Out of 193 X-rayed eels, 107 contained parasites; 27 infections were undetectable on radiographs (false negatives). Detection was influenced by the intensity, size, and location of parasites within the swimbladder. This digital X-ray method is a quick and non-lethal process that could be incorporated into existing monitoring programs.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"161 ","pages":"87-93"},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spirometry as a diagnostic tool to assess respiratory health in beached bottlenose dolphins Tursiops spp. 肺量测定法作为诊断工具评估海滩宽吻海豚呼吸健康。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-03-20 DOI: 10.3354/dao03843

A Fahlman, B Sterba-Boatwright, F Cauture, J Sweeney, R Stone

{"title":"Spirometry as a diagnostic tool to assess respiratory health in beached bottlenose dolphins Tursiops spp.","authors":"A Fahlman, B Sterba-Boatwright, F Cauture, J Sweeney, R Stone","doi":"10.3354/dao03843","DOIUrl":"10.3354/dao03843","url":null,"abstract":"In this study, we used a dataset including 42 individual bottlenose dolphins (Tursiops spp.) to determine the reliability of lung function testing as a method for assessing respiratory health. Each dolphin was trained to beach voluntarily, allowing researchers to measure respiratory flow in a controlled, beached state. From the collected respiratory flow data, alongside timing parameters, we extracted 18 specific variables, supplemented by additional factors such as body mass, age, and sex. These variables were hypothesized to serve as potential variables for identifying respiratory compromise. A model was developed that reduced the number of predictive variables, showing that 4 specific variables were particularly effective, yielding an accuracy of 88.4% in determining whether a dolphin was free from respiratory disease. This high level of accuracy underscores the potential of lung function testing as a diagnostic tool in the context of stranded dolphins, where rapid, non-invasive methods are crucial for assessing health status. These results suggest that lung function testing provides a non-invasive and efficient method for evaluating respiratory health in stranded dolphins and supports the use of lung function assessments in wildlife management and conservation. By enabling early detection of respiratory issues, this approach can enhance the success of rehabilitation efforts, potentially improving the survival rates of dolphins that have stranded, which is often a critical concern in marine conservation initiatives.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"161 ","pages":"113-124"},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metastatic gastric adenocarcinoma in a grey seal Halichoerus grypus: clinicopathological and immunohistochemical characterization. 灰海豹斑纹海狮转移性胃腺癌：临床病理和免疫组织化学特征。

IF 1.1 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-03-20 DOI: 10.3354/dao03845

Gonçalo Nogueira Marques, Bernat Marti-Garcia, Miriam Oliveira Leal, Nuno Urbani Ramos da Silva, Carla Anne Flanagan, Alejandro Suárez-Bonnet

{"title":"Metastatic gastric adenocarcinoma in a grey seal Halichoerus grypus: clinicopathological and immunohistochemical characterization.","authors":"Gonçalo Nogueira Marques, Bernat Marti-Garcia, Miriam Oliveira Leal, Nuno Urbani Ramos da Silva, Carla Anne Flanagan, Alejandro Suárez-Bonnet","doi":"10.3354/dao03845","DOIUrl":"10.3354/dao03845","url":null,"abstract":"A 33 yr old female grey seal Halichoerus grypus presented with inappetence and progressive weight loss. Medical management included blood analysis, imaging, and fecal evaluation, along with multimodal supportive therapy, which periodically improved the overall medical condition. Six months after the initial presentation, the clinical condition deteriorated significantly, including severe hyporexia, hematemesis, and marked neutrophilic leukocytosis, which led to the decision to euthanise based on welfare grounds. Necropsy findings included severe thickening of the distal esophagus, cardia, and proximal gastric fundus, as well as multiple nodular to cystic structures over the stomach's serosa, omentum, and mesentery. Histologically, a mucinous gastric adenocarcinoma was diagnosed, with metastasis to the gastric lymph nodes and prominent carcinomatosis involving the omentum, mesentery, and diaphragm. Immunohistochemically, the gastric adenocarcinoma was positive for cytokeratin AE1/AE3, weakly positive for COX-2 and E-cadherin, and negative for vimentin. The Ki-67 proliferative index was low (0.8). Although rare, this case offers further insights into the clinical presentation, histopathology, and immunohistochemical profile of gastric tumors in pinnipeds.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"161 ","pages":"125-131"},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0