奇努克鲑鱼体内沙门氏菌的组织学进展和细菌负荷动态。

IF 1.1 4区 农林科学 Q3 FISHERIES
Riley E Dils, Tawni B R Firestone, Paula A Schaffer, Dana L Winkelman, Eric R Fetherman
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引用次数: 0

摘要

沙门氏菌是细菌性肾病(BKD)的病因,严重影响沙门氏菌种群。我们对鲑鱼BKD病理的了解大多来自对野生种群或产卵的太平洋西北鲑鱼致命感染的评估。本研究研究了奇努克鲑鱼腹腔注射后沙门氏菌感染的组织学进展和细菌负荷动态,以提高对疾病动力学的认识。用ATCC-33209分离株对70条鲑鱼进行了10周的监测。在注射后1周观察到脾脏和肝脏组织的组织学病变,在注射后4周观察到肾脏组织的病变,随着时间的推移,疾病的体征逐渐增加。此外,组织病理学分析显示脾和肝包膜炎(体腔炎),单个肝细胞坏死,进行性肉芽肿性肝炎,脾炎和肾炎伴坏死。革兰氏染色证实感染组织中巨噬细胞内和细胞外存在革兰氏阳性菌。定量PCR显示,在研究期间,肾脏和肝脏组织中的细菌负荷显著增加,肾脏组织中观察到更高的细菌负荷。细菌负荷与疾病分期密切相关,细菌负荷高峰与最严重的组织学改变相吻合。我们的研究提供了一个全面的帐户沙门氏菌感染和疾病进展后,奇努克鲑鱼腹腔注射。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histological progression and bacterial load dynamics of Renibacterium salmoninarum in Chinook salmon Oncorhynchus tshawytscha.

Renibacterium salmoninarum, the cause of bacterial kidney disease (BKD), severely impacts salmonid populations. Much of our understanding of the BKD pathology in salmonids comes from evaluating fatal infections in wild populations or spawning Pacific Northwest salmonids. Our study investigated the histological progression and bacterial load dynamics of R. salmoninarum infection in Chinook salmon following intraperitoneal injection to enhance understanding of the disease's dynamics. Seventy presmolt salmon were injected with the ATCC-33209 isolate of R. salmoninarum and monitored over 10 wk. Histological signs of disease were observed in spleen and liver tissues at 1 wk post-injection and in kidney tissues at 4 wk post-injection, with signs of disease increasing over time. Additionally, histopathological analysis revealed splenic and hepatic capsulitis (coelomitis), individual hepatocyte necrosis, progressive granulomatous hepatitis, splenitis, and nephritis with necrosis. Gram staining confirmed the presence of Gram-positive bacteria within macrophages and extracellularly in infected tissues. Quantitative PCR revealed significant increases in bacterial loads in both kidney and liver tissues over the study period, with higher bacterial loads observed in the kidney tissue. Bacterial load was strongly correlated with disease stage, with peak bacterial burdens coinciding with the most severe histological changes. Our study provides a comprehensive account of R. salmoninarum infection and disease progression in Chinook salmon after intraperitoneal injection.

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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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