Riley E Dils, Tawni B R Firestone, Paula A Schaffer, Dana L Winkelman, Eric R Fetherman
{"title":"奇努克鲑鱼体内沙门氏菌的组织学进展和细菌负荷动态。","authors":"Riley E Dils, Tawni B R Firestone, Paula A Schaffer, Dana L Winkelman, Eric R Fetherman","doi":"10.3354/dao03852","DOIUrl":null,"url":null,"abstract":"<p><p>Renibacterium salmoninarum, the cause of bacterial kidney disease (BKD), severely impacts salmonid populations. Much of our understanding of the BKD pathology in salmonids comes from evaluating fatal infections in wild populations or spawning Pacific Northwest salmonids. Our study investigated the histological progression and bacterial load dynamics of R. salmoninarum infection in Chinook salmon following intraperitoneal injection to enhance understanding of the disease's dynamics. Seventy presmolt salmon were injected with the ATCC-33209 isolate of R. salmoninarum and monitored over 10 wk. Histological signs of disease were observed in spleen and liver tissues at 1 wk post-injection and in kidney tissues at 4 wk post-injection, with signs of disease increasing over time. Additionally, histopathological analysis revealed splenic and hepatic capsulitis (coelomitis), individual hepatocyte necrosis, progressive granulomatous hepatitis, splenitis, and nephritis with necrosis. Gram staining confirmed the presence of Gram-positive bacteria within macrophages and extracellularly in infected tissues. Quantitative PCR revealed significant increases in bacterial loads in both kidney and liver tissues over the study period, with higher bacterial loads observed in the kidney tissue. Bacterial load was strongly correlated with disease stage, with peak bacterial burdens coinciding with the most severe histological changes. Our study provides a comprehensive account of R. salmoninarum infection and disease progression in Chinook salmon after intraperitoneal injection.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"85-97"},"PeriodicalIF":1.1000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Histological progression and bacterial load dynamics of Renibacterium salmoninarum in Chinook salmon Oncorhynchus tshawytscha.\",\"authors\":\"Riley E Dils, Tawni B R Firestone, Paula A Schaffer, Dana L Winkelman, Eric R Fetherman\",\"doi\":\"10.3354/dao03852\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Renibacterium salmoninarum, the cause of bacterial kidney disease (BKD), severely impacts salmonid populations. Much of our understanding of the BKD pathology in salmonids comes from evaluating fatal infections in wild populations or spawning Pacific Northwest salmonids. Our study investigated the histological progression and bacterial load dynamics of R. salmoninarum infection in Chinook salmon following intraperitoneal injection to enhance understanding of the disease's dynamics. Seventy presmolt salmon were injected with the ATCC-33209 isolate of R. salmoninarum and monitored over 10 wk. Histological signs of disease were observed in spleen and liver tissues at 1 wk post-injection and in kidney tissues at 4 wk post-injection, with signs of disease increasing over time. Additionally, histopathological analysis revealed splenic and hepatic capsulitis (coelomitis), individual hepatocyte necrosis, progressive granulomatous hepatitis, splenitis, and nephritis with necrosis. Gram staining confirmed the presence of Gram-positive bacteria within macrophages and extracellularly in infected tissues. Quantitative PCR revealed significant increases in bacterial loads in both kidney and liver tissues over the study period, with higher bacterial loads observed in the kidney tissue. Bacterial load was strongly correlated with disease stage, with peak bacterial burdens coinciding with the most severe histological changes. Our study provides a comprehensive account of R. salmoninarum infection and disease progression in Chinook salmon after intraperitoneal injection.</p>\",\"PeriodicalId\":11252,\"journal\":{\"name\":\"Diseases of aquatic organisms\",\"volume\":\"162 \",\"pages\":\"85-97\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-05-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diseases of aquatic organisms\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3354/dao03852\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diseases of aquatic organisms","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3354/dao03852","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}
Histological progression and bacterial load dynamics of Renibacterium salmoninarum in Chinook salmon Oncorhynchus tshawytscha.
Renibacterium salmoninarum, the cause of bacterial kidney disease (BKD), severely impacts salmonid populations. Much of our understanding of the BKD pathology in salmonids comes from evaluating fatal infections in wild populations or spawning Pacific Northwest salmonids. Our study investigated the histological progression and bacterial load dynamics of R. salmoninarum infection in Chinook salmon following intraperitoneal injection to enhance understanding of the disease's dynamics. Seventy presmolt salmon were injected with the ATCC-33209 isolate of R. salmoninarum and monitored over 10 wk. Histological signs of disease were observed in spleen and liver tissues at 1 wk post-injection and in kidney tissues at 4 wk post-injection, with signs of disease increasing over time. Additionally, histopathological analysis revealed splenic and hepatic capsulitis (coelomitis), individual hepatocyte necrosis, progressive granulomatous hepatitis, splenitis, and nephritis with necrosis. Gram staining confirmed the presence of Gram-positive bacteria within macrophages and extracellularly in infected tissues. Quantitative PCR revealed significant increases in bacterial loads in both kidney and liver tissues over the study period, with higher bacterial loads observed in the kidney tissue. Bacterial load was strongly correlated with disease stage, with peak bacterial burdens coinciding with the most severe histological changes. Our study provides a comprehensive account of R. salmoninarum infection and disease progression in Chinook salmon after intraperitoneal injection.
期刊介绍:
DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically:
-Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens
-Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)-
Diseases due to internal circumstances (innate, idiopathic, genetic)-
Diseases due to proliferative disorders (neoplasms)-
Disease diagnosis, treatment and prevention-
Molecular aspects of diseases-
Nutritional disorders-
Stress and physical injuries-
Epidemiology/epizootiology-
Parasitology-
Toxicology-
Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)-
Diseases as indicators of humanity''s detrimental impact on nature-
Genomics, proteomics and metabolomics of disease-
Immunology and disease prevention-
Animal welfare-
Zoonosis