Avian Pathology最新文献

Atypical Salmonella Infantis variants display different shedding and colonization behaviour in broiler chickens and bear the ability to revert to motility. 非典型婴儿沙门氏菌变体在肉鸡中表现出不同的脱落和定植行为，并具有恢复活力的能力。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-09-01 DOI: 10.1080/03079457.2026.2691985

Mohammad Alitabar, Victoria Drauch, Christian Kornschober, Liča Lozica, Michael Hess, Claudia Hess

引用次数: 0

Establishment of a rapid detection method for Tembusu virus based on MIRA-PfAgo. 基于MIRA-PfAgo的坦布苏病毒快速检测方法的建立。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-27 DOI: 10.1080/03079457.2026.2701311

Bei Wang, Liwei Liu, Xiaoqing Wang, Kai Yang, Daojun Chen, Dongdong Yin

{"title":"Establishment of a rapid detection method for Tembusu virus based on MIRA-PfAgo.","authors":"Bei Wang, Liwei Liu, Xiaoqing Wang, Kai Yang, Daojun Chen, Dongdong Yin","doi":"10.1080/03079457.2026.2701311","DOIUrl":"10.1080/03079457.2026.2701311","url":null,"abstract":"In this study, a novel nucleic acid detection method based on multienzyme isothermal rapid amplification (MIRA) combined with the Pyrococcus furiosus Argonaute (PfAgo) cleavage system (MIRA-PfAgo) was developed for the detection of Tembusu virus (TMUV), an important pathogen threatening the waterfowl industry. The method targets the highly conserved NS5 gene of TMUV. Target nucleic acids are rapidly enriched by MIRA under isothermal conditions at 39°C, followed by PfAgo-mediated cleavage guided by sequence-specific guide DNA (gDNA), enabling visual detection through fluorescence signal output. Systematic optimization identified 0.5 μM gDNA, 1 μM PfAgo, and 5 mM Mn²⁺ as the optimal reaction conditions. The assay exhibited excellent specificity for TMUV, with no cross-reactivity observed with common waterfowl pathogens, including H9 subtype avian influenza virus (AIV-H9), duck enteritis virus (DEV), goose parvovirus (GPV), Muscovy duck reovirus (MDRV), Newcastle disease virus (NDV), and duck circovirus (DuCV). The sensitivity was 10⁰ copies/μl. In the analysis of 21 clinical samples, the results of the MIRA-PfAgo assay were fully consistent with those obtained by RT-PCR. This method does not require complex thermal cycling equipment and combines high sensitivity, strong specificity, and operational simplicity, providing a reliable and practical technical tool for rapid on-site monitoring of TMUV.RESEARCH HIGHLIGHTSThis is the first study to utilize the MIRA-PfAgo detection assay for the detection of TMUV.The results are observable by the unaided eye.The developed assay potentially offers an alternative means for detection of TMUV, requiring minimal equipment.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.7,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological survey of domestic pigeons, free-ranging feral pigeons (both Columba livia) and wild common wood pigeons (Columba palumbus) for rotavirus A (RVA) infections in Germany. 德国家鸽、散养野鸽（Columba livia）和野生普通木鸽（Columba palumbus）轮状病毒A （RVA）感染的血清学调查。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-07 DOI: 10.1080/03079457.2026.2699977

Ole Pietsch, Luisa Fischer, Elisabeth Peus, Franziska Schopf, Florentin Ressel, Laura Pahnke, Ute Ziegler, Dennis Rubbenstroth

引用次数: 0

Genetic characterization and zoonotic potential of G1-Lineage H9N2 avian influenza viruses isolated from poultry in Pakistan, 2023. 2023年巴基斯坦家禽中分离的g1系H9N2禽流感病毒的遗传特征和人畜共患潜力

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-07 DOI: 10.1080/03079457.2026.2699158

Kai-Yun Tan, Nousheen Arshad, Salman L Butt, Sun-Hak Lee, Ye-Ram Seo, Asif M Rana, Bitgoeul Kim, Chung-Young Lee, Dong-Hun Lee

{"title":"Genetic characterization and zoonotic potential of G1-Lineage H9N2 avian influenza viruses isolated from poultry in Pakistan, 2023.","authors":"Kai-Yun Tan, Nousheen Arshad, Salman L Butt, Sun-Hak Lee, Ye-Ram Seo, Asif M Rana, Bitgoeul Kim, Chung-Young Lee, Dong-Hun Lee","doi":"10.1080/03079457.2026.2699158","DOIUrl":"https://doi.org/10.1080/03079457.2026.2699158","url":null,"abstract":"The H9N2 subtype of avian influenza A virus has been endemic in poultry populations across Asia and the Middle East, posing ongoing economic and public health concerns. Since their initial detection in Pakistan in the 1990s, H9N2 viruses have caused repeated outbreaks in commercial poultry, leading to substantial economic losses and raising concerns about zoonotic transmission. To characterize the recent genetic evolution and zoonotic potential of circulating strains, five H9N2 isolates were obtained from poultry in Pakistan between January and March 2023. Phylogenetic analysis of the haemagglutinin gene revealed that all five isolates belong to the G5.3.2, formerly known as B2, sub-lineage of the G1 Eurasian lineage, in accordance with the revised global classification system for H9 viruses. Comparative genomic analysis confirmed that all eight gene segments were closely related to previously reported Pakistani strains, with no evidence of recent reassortment, suggesting localized persistence and ongoing genetic drift. Notably, several mammalian-adaptive mutations were identified in internal gene segments of the isolates, suggesting a potential risk of cross-species transmission. In addition, all five isolates exhibited dual receptor-binding characteristics, recognizing both α2,3-linked (avian-type) and α2,6-linked (human-type) sialic acid glycans, with a stronger affinity for α2,6-linked glycans. These findings underscore the need for continuous surveillance and risk assessment of H9N2 viruses circulating in Pakistan.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-12"},"PeriodicalIF":2.7,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148688413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a reassortant infectious bursal disease virus (genotype A3B1) in the United Kingdom: pathogenicity and immune complex vaccine efficacy. 在英国鉴定一种重组传染性法氏囊病病毒（基因型A3B1）：致病性和免疫复合物疫苗效力。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-03 DOI: 10.1080/03079457.2026.2701308

Gregori Bech, Elena Marzo, Christian Blake-Dyke, Marta Busquet, Michelle Woodward, Marta Alech, Marina Solé, Alba Sánchez, Eduard Sebastià

{"title":"Identification of a reassortant infectious bursal disease virus (genotype A3B1) in the United Kingdom: pathogenicity and immune complex vaccine efficacy.","authors":"Gregori Bech, Elena Marzo, Christian Blake-Dyke, Marta Busquet, Michelle Woodward, Marta Alech, Marina Solé, Alba Sánchez, Eduard Sebastià","doi":"10.1080/03079457.2026.2701308","DOIUrl":"10.1080/03079457.2026.2701308","url":null,"abstract":"The immunosuppressive effects of the infectious bursal disease virus (IBDV), together with its ongoing genome evolution, still represent a major challenge for poultry farmers. This study characterized a novel reassortant IBDV strain, named UK2020, which was isolated in broiler farms from the United Kingdom in 2020 and has now spread across Europe. The phylogenetic analysis revealed that UK2020 belongs to the genotype A3B1, with segment A derived from a very virulent strain (vvIBDV) and segment B from a classical attenuated virus. The pathogenicity studies conducted in both specific-pathogen-free and commercial broiler chickens, with and without maternally-derived antibodies (MDAs), demonstrated that UK2020 caused subclinical infections. These infections resulted in splenomegaly, moderate inflammation of the bursa with histopathological changes, such as lymphoid depletion, inflammatory cell infiltration and plical oedema, and a significant humoral response, despite the absence of clinical signs. The efficacy of a commercial IBD immune complex (Icx) vaccine was also evaluated. Vaccinated birds showed significantly higher antibody titres and reduced lymphoid organ damage compared to mock-vaccinated controls. These results highlight the need for continuous field surveillance to monitor the circulation and impact of reassortant IBDV strains under the current control programmes. The complete sequencing of segments A and B is essential for accurate detection and classification of such strains. The use of Icx vaccines may contribute to mitigating bursal and splenic lesions associated with A3B1 infections like UK2020, especially in flocks with variable MDA levels.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-13"},"PeriodicalIF":2.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early challenge with LPAI-H9N2 followed by vNDV-VII.1.1 in vaccinated and unvaccinated broilers: a comprehensive study. 在接种疫苗和未接种疫苗的肉鸡中，早期用LPAI-H9N2攻毒，随后用vNDV-VII.1.1攻毒：一项综合研究

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-06-24 DOI: 10.1080/03079457.2026.2674853

Ahmed Elbestawy, Eman Khalifa, Adel H Saad, Ahmed R Elnagar, Hany F Ellakany, Mervat Abdel-Latif, Ahmed E Noreldin, Amr Geneedy, Ahmed Gado

引用次数: 0

Genetic and vaccination studies reveal feather viral load as a potential biomarker for individual and flock-level protection against Marek's disease in chicken. 遗传和疫苗研究表明，羽毛病毒载量是鸡个体和群体预防马立克病的潜在生物标志物。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-06-22 DOI: 10.1080/03079457.2026.2661686

Hector Marina, Richard I Bailey, Jody Mays, Marie Ithurbide, Margo Chase-Topping, Hans Cheng, John R Dunn, Andrea Doeschl-Wilson

引用次数: 0

Editorial introduction. 编辑介绍。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-07-13 DOI: 10.1080/03079457.2026.2677382

Magdy F Elkady, Ahmed R Elbestawy, Kareem Eid

引用次数: 0

Outbreak dynamics of high pathogenicity avian influenza virus H5N1, clade 2.3.4.4b euBB, in black-headed gulls and common terns in Germany in 2023. 2023年德国黑头鸥和普通候鸟中高致病性禽流感病毒H5N1，分支2.3.4.4b euBB的爆发动态

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2026-06-08 DOI: 10.1080/03079457.2026.2659834

Ulrich Knief, Ann Kathrin Ahrens, Valerie Allendorf, Carla J Behringer, Justine Bertram, Sandra Bouwhuis, Wolfgang Fiedler, Anja Globig, Anne Günther, Christof Herrmann, Sascha Knauf, Dominik Marchowski, Simon Piro, Anne Pohlmann, Robert E Rollins, Christoph Staubach, Timm Harder

引用次数: 0

Antibiotic resistance and first comprehensive genomic characteristics of Salmonella Gallinarum isolated from poultry in Morocco. 摩洛哥家禽鸡源沙门氏菌的抗生素耐药性和首次综合基因组特征。

IF 2.7 2区农林科学

Avian Pathology Pub Date : 2026-08-01 Epub Date: 2025-10-31 DOI: 10.1080/03079457.2025.2562221

Mouad Farhat, Slimane Khayi, Mohamed Mouahid, Najia Ameur, Hosny El-Adawy, Siham Fellahi

引用次数: 0