Avian Pathology最新文献

{"title":"Comparative genomic analysis reveals the emergence and dissemination of different <i>Salmonella enterica</i> serovar Gallinarum biovar Gallinarum lineages in Brazil.","authors":"Diéssy Kipper, Renato Hohl Orsi, Nathalie de Souza Zanetti, Silvia De Carli, Andrea Karoline Mascitti, André Salvador Kazantzi Fonseca, Nilo Ikuta, Martin Wiedmann, Vagner Ricardo Lunge","doi":"10.1080/03079457.2025.2458601","DOIUrl":"10.1080/03079457.2025.2458601","url":null,"abstract":"<p><strong>Research highlights: </strong>Fowl typhoid (FT) is a concerning poultry disease caused by <i>S.</i> Gallinarum.Five <i>S.</i> Gallinarum lineages (I to V) were demonstrated in South American farms.<i>S.</i> Gallinarum lineages have specific antimicrobial resistance / virulence genomic profiles.Main FT outbreaks in Brazil have been caused by the specific lineage II.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"476-488"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient differentiation between <i>Salmonella</i> Pullorum and <i>Salmonella</i> Gallinarum by a <i>fimH</i>-based PCR-HRM.","authors":"Di Zhang, Linlin Zhuang, Yi Jiang, Yi Yang, Ming Xu, Xinhong Dou, Jiansen Gong","doi":"10.1080/03079457.2025.2450840","DOIUrl":"10.1080/03079457.2025.2450840","url":null,"abstract":"<p><p><i>Salmonella</i> Pullorum (<i>S</i>. Pullorum) and <i>Salmonella</i> Gallinarum (<i>S</i>. Gallinarum) are the biovars of <i>Salmonella enterica</i> serovar Gallinarum that are responsible for pullorum disease and fowl typhoid, respectively, in poultry. Traditional serological methods fail to quickly differentiate between these biovars due to their identical O antigenic factors (O9 and O12). Although single nucleotide polymorphism (SNP)-based methods have been used to distinguish between the biovars, they often lack the required accuracy and effectiveness. In this study, we developed a PCR high resolution melt (PCR-HRM) assay, which targeted a SNP at position 665 of the <i>fimH</i> gene, for rapid differentiation between <i>S</i>. Pullorum and <i>S</i>. Gallinarum. Our method showed 100% specificity and was able to detect as little as 0.033 pg of <i>S</i>. Pullorum DNA and 0.027 pg of <i>S</i>. Gallinarum DNA. The PCR-HRM results for 547 clinical isolates were in complete agreement with traditional serological methods. This PCR-HRM assay significantly reduced identification time and provided high throughput, efficient testing. This makes it a practical and reliable tool for accurate differentiation between <i>S</i>. Pullorum and <i>S</i>. Gallinarum in clinical settings.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"450-454"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and efficacy of an inactivated tetravalent water-in-oil emulsion <i>Escherichia coli</i> vaccine against the <i>E. coli</i> peritonitis syndrome.","authors":"W J M Landman, J H H van Eck, M Schellekens, A Feberwee","doi":"10.1080/03079457.2024.2448510","DOIUrl":"10.1080/03079457.2024.2448510","url":null,"abstract":"<p><strong>Research highlights: </strong>Protection against <i>E. coli</i> (EPS) challenge seems to be genotype-serotype-specific.Genotype B (O78:H4) gave (almost) full protection against genotypes B, F and H (all O78:H4).Genotype D (O11:H12) incited partial protection.Genotypes A (O1:H7) and C (O2:H1) were not protective.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"427-437"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-infections of <i>Enterococcus cecorum</i> and various avian pathogens resulted in varying rates of SPF broilers with an <i>E. cecorum</i> infection.","authors":"Thijs Manders, Merlijn Kense, Remco Dijkman, Jeanine Wiegel, Mieke Matthijs, Sjaak de Wit","doi":"10.1080/03079457.2025.2450428","DOIUrl":"10.1080/03079457.2025.2450428","url":null,"abstract":"<p><strong>Research highlights: </strong>Typical <i>E. cecorum</i> lesions can be reproduced in SPF broilers after intravenous, aerosol and oral inoculations.The respiratory route is potentially an infection route for pathogenic <i>E. cecorum</i> bacteria.Co-infections tested in this study or dexamethasone do not exacerbate the proportion of <i>E. cecorum</i> lesions.M.s. in combination with IBV or NDV vaccines exacerbates the proportion of positive reisolations.Immunosuppression induced by early CAV infection increases the proportion of positive reisolations.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"438-449"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the efficacy of <i>Mycoplasma gallisepticum</i> vaccine programmes in chickens.","authors":"Naola Ferguson-Noel, Marianne Dos Santos, Mohammadreza Ehsan, Eniope B Oluwayinka","doi":"10.1080/03079457.2024.2443508","DOIUrl":"10.1080/03079457.2024.2443508","url":null,"abstract":"<p><strong>Research highlights: </strong>Vaccine programmes for <i>Mycoplasma gallisepticum</i> were compared in layer chickens.Vaccine programmes included combinations of live F-strain vaccine and bacterin.All vaccine programmes showed reduced tracheal colonization with challenge strain.Live and bacterin combined significantly reduced lesions compared to other groups.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"385-397"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological characteristics of keel bone maturation and keel bone fractures in laying hens.","authors":"Páll Gretarsson, Käthe Kittelsen, Randi Oppermann Moe, Ingrid Toftaker, Ida Thøfner","doi":"10.1080/03079457.2024.2448498","DOIUrl":"10.1080/03079457.2024.2448498","url":null,"abstract":"<p><p>The keel bone in laying hens is prone to fractures, especially on the caudal third of the keel, which is also the last part to ossify. Keel bone fractures (KBF) typically occur between 25 and 50 weeks of age (WOA). However, the keel is fully ossified at around 40 WOA, suggesting fractures can occur before ossification is complete. To better understand the relationship between KBF and ossification, this descriptive study examined keel bone morphology during maturation. Keel bones from 50 commercial aviary housed Dekalb White laying hens were collected at 10 timepoints from 17-53 WOA and prepared for histological analysis. The samples were stained with haematoxylin and eosin and Safranin O to show cartilage, ossification, bone tissue, and KBF. The results indicated an ossification process similar to endochondral ossification. The degree of ossification varied between individuals of the same age. The age at complete ossification varied from 28-49 WOA. None of the keels from hens aged 53 WOA were fully ossified. Cartilage canals were present in the keel cartilage. Medullary bone was observed in all age groups. Most fractures lacked tissue morphologies typical of high-impact collisions, suggesting the need for further research into the underlying causes. This is the first study to detail keel bone histomorphology in commercial laying hens, providing baseline data for future research.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"411-426"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and structural factors contributing to the dominance and persistence of goose astrovirus type 2.","authors":"Peng Jiang, Aolin Li, Hongfu Ma, Yankun Lan, Jiaguo Wang, Xin Wang, Liang Zhao, Qifei Wu, Yong Wang, Xu Guo","doi":"10.1080/03079457.2025.2454416","DOIUrl":"10.1080/03079457.2025.2454416","url":null,"abstract":"<p><strong>Research highlights: </strong>Goose astrovirus type 2 (GoAstV-2) became the dominant strain in China post-2017.GoAstV-2 exhibits weaker codon usage bias, enhancing adaptability across hosts.The stability of GoAstV-2 spike protein reduces mutation needs and selective pressure.GoAstV-2 low spike protein variability supports long-term persistence in host populations.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"455-463"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of immunopathological changes in the feather pulp of CVI988-vaccinated pullets challenged with a very virulent plus Marek's disease virus strain.","authors":"Federico C Bonorino, Juan Francisco Garcia Marin, Abdelhamid Fares, Nagwa Khaled, Deanna Emmanuel, Raveendra R Kulkarni, Isabel Gimeno","doi":"10.1080/03079457.2025.2472838","DOIUrl":"10.1080/03079457.2025.2472838","url":null,"abstract":"<p><p>High load of oncogenic Marek's disease virus (MDV) DNA in the feather pulp (FP) as early as 21 days of age is a powerful criterion to predict the outcome of Marek's disease (MD) in apparently healthy chickens. The objective of this study was to elucidate the immunopathological changes in the FP of 21-day-old chickens that had been vaccinated with CVI988 vaccine (healthy), vaccinated and challenged with a very virulent plus (vv+) MDV strain 648A (well protected), or were unvaccinated and challenged with 648A strain (not protected) when compared to uninfected naïve chickens. Oncogenic MDV DNA load, histopathological and immunohistochemical evaluation of the lesions, and immunophenotypic characterization of the infiltrates by flow cytometry were examined. Our results demonstrate that 648A-infected unvaccinated chickens had a significant increase in the percentage of CD3+ T cells, mainly CD4+MHC-II+ cells and CD8+MHC-II+ cells, when compared to all other groups. They also had a significantly decreased number of CD8β+ T cells compared to all other groups. Infection with 648A reduced the percentage of macrophages, not only in the unvaccinated group but also in the CVI988/648A group. In addition, groups that were vaccinated with CVI988, regardless of the challenge status, had higher levels of CD8β+ T cells, suggesting that the vaccine has an enhancing effect on the CTL cells. Our results showed that the load of oncogenic MDV is highly correlated with the infiltration of CD4+MHC-II+ cells and provide further confirmation that FP is indeed an appropriate sample for the early diagnosis of MD.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"521-529"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircITSN2-miR-17-5p/20a-5p/20b-5p-<i>PD-L1</i> regulatory network is a potential molecular mechanism of <i>PD-L1</i> gene involving in immune response to IBDV.","authors":"Yi Jiang, Yufei Tian, Jianwei Han, Xiangnan Wang, Rui Zhang, Xinxin Xu, Xiaoli Ma, Wei Zhang, Chaolai Man","doi":"10.1080/03079457.2025.2470754","DOIUrl":"10.1080/03079457.2025.2470754","url":null,"abstract":"<p><strong>Research highlights: </strong><i>PD-L1</i> gene is correlated with IBDV immune response in chickens.<i>PD-L1</i> is a key gene regulating the immune functions of the heart, lung, and proventriculus.CircITSN2-miR-17-5p/20a-5p/20b-5p-<i>PD-L1</i> is a potential mechanism in IBDV immunity.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"512-520"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of highly pathogenic avian influenza virus (HPAIV) on Black-headed Gulls <i>Chroicocephalus ridibundus</i> population in Poland in 2023.","authors":"Piotr Indykiewicz, Marcin Przymencki, Piotr Minias, Dariusz Jakubas, Klaudia Litwiniak, Piotr Zieliński, Tomasz Janiszewski, Radosław Włodarczyk, Mateusz Ledwoń, Jacek Nowakowski, Beata Dulisz, Katarzyna Domańska-Blicharz, Edyta Świętoń, Krzysztof Śmietanka, Dariusz Bukaciński, Monika Bukacińska, Arkadiusz Buczyński, Szymon Beuch, Tomasz Chodkiewicz, Jacek Betleja, Szymon Bzoma, Dominik Marchowski, Arkadiusz Sikora, Łukasz Bednarz, Jacek Antczak, Andrzej Dylik, Zbigniew Kajzer, Wiesław Król, Łukasz Krajewski, Sebastian Menderski, Jan Rapczyński, Sławomir Rubacha, Rafał Szczerbik, Jakub Szymczak, Kazimierz Walasz, Przemysław Wylegała","doi":"10.1080/03079457.2025.2467122","DOIUrl":"10.1080/03079457.2025.2467122","url":null,"abstract":"<p><p>The highly pathogenic avian influenza virus (HPAIV) A(H5N1) has caused the most extensive and severe epizootic event affecting both poultry and wild birds globally. This study investigated the impact of HPAIV on the breeding population of the Black-headed Gull <i>Chroicocephalus ridibundus</i>, the most abundant gull species in Poland. During the 2023 outbreak, this species was reported as the most frequently infected in the country. A higher-than-natural adult mortality rate (greater than 1.5% of the breeding individuals) was observed in 114 surveyed colonies across all regions of Poland. Laboratory tests confirmed the presence of HPAIV in all 17 colonies sampled, with average adult mortality estimated at 26.1%, and ranging from 1.7% to 77.8%. The estimated mortality rate across all surveyed colonies was 22.2%. Extrapolations across the entire Polish breeding population (at least 115,000 pairs according to the national census) indicated that approximately 51,000 adult Black-headed Gulls might have perished due to HPAIV in 2023. The number of adults found dead was positively correlated with colony size (<i>r</i> = 0.733, <i>P</i> < 0.001). The deaths were associated with a single HPAIV genotype (BB) across all confirmed cases. Understanding the spread and severity of HPAIV in colonially breeding waterbirds, such as gulls, is essential for assessing the full extent of the threats this virus poses to wild bird populations.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"498-511"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}