Avian Pathology最新文献

{"title":"Characterization of immunopathological changes in the feather pulp of CVI988-vaccinated pullets challenged with a very virulent plus Marek's disease virus strain.","authors":"Federico C Bonorino, Juan Francisco Garcia Marin, Abdelhamid Fares, Nagwa Khaled, Deanna Emmanuel, Raveendra R Kulkarni, Isabel Gimeno","doi":"10.1080/03079457.2025.2472838","DOIUrl":"10.1080/03079457.2025.2472838","url":null,"abstract":"<p><p>High load of oncogenic Marek's disease virus (MDV) DNA in the feather pulp (FP) as early as 21 days of age is a powerful criterion to predict the outcome of Marek's disease (MD) in apparently healthy chickens. The objective of this study was to elucidate the immunopathological changes in the FP of 21-day-old chickens that had been vaccinated with CVI988 vaccine (healthy), vaccinated and challenged with a very virulent plus (vv+) MDV strain 648A (well protected), or were unvaccinated and challenged with 648A strain (not protected) when compared to uninfected naïve chickens. Oncogenic MDV DNA load, histopathological and immunohistochemical evaluation of the lesions, and immunophenotypic characterization of the infiltrates by flow cytometry were examined. Our results demonstrate that 648A-infected unvaccinated chickens had a significant increase in the percentage of CD3+ T cells, mainly CD4+MHC-II+ cells and CD8+MHC-II+ cells, when compared to all other groups. They also had a significantly decreased number of CD8β+ T cells compared to all other groups. Infection with 648A reduced the percentage of macrophages, not only in the unvaccinated group but also in the CVI988/648A group. In addition, groups that were vaccinated with CVI988, regardless of the challenge status, had higher levels of CD8β+ T cells, suggesting that the vaccine has an enhancing effect on the CTL cells. Our results showed that the load of oncogenic MDV is highly correlated with the infiltration of CD4+MHC-II+ cells and provide further confirmation that FP is indeed an appropriate sample for the early diagnosis of MD.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircITSN2-miR-17-5p/20a-5p/20b-5p-<i>PD-L1</i> regulatory network is a potential molecular mechanism of <i>PD-L1</i> gene involving in immune response to IBDV.","authors":"Yi Jiang, Yufei Tian, Jianwei Han, Xiangnan Wang, Rui Zhang, Xinxin Xu, Xiaoli Ma, Wei Zhang, Chaolai Man","doi":"10.1080/03079457.2025.2470754","DOIUrl":"10.1080/03079457.2025.2470754","url":null,"abstract":"<p><strong>Research highlights: </strong><i>PD-L1</i> gene is correlated with IBDV immune response in chickens.<i>PD-L1</i> is a key gene regulating the immune functions of the heart, lung, and proventriculus.CircITSN2-miR-17-5p/20a-5p/20b-5p-<i>PD-L1</i> is a potential mechanism in IBDV immunity.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant autobioluminescent <i>Escherichia coli</i> to monitor the progression of <i>Escherichia coli</i> infection in the embryonated chicken eggs.","authors":"Mohamed Kamal Abdelhamid, Surya Paudel, Hammad Ur Rehman, Manolis Lyrakis, Ivana Bilic, Michael Hess, Claudia Hess","doi":"10.1080/03079457.2025.2477246","DOIUrl":"https://doi.org/10.1080/03079457.2025.2477246","url":null,"abstract":"<p><p><b>Abstract</b>Avian pathogenic <i>Escherichia coli</i> (APEC) infections in poultry adversely affect health and production, with public health implications. This study assessed the potential of bioluminescence imaging for real-time, noninvasive tracking of microbial progression in 12-day-old chicken embryos inoculated with an APEC strain or its derivatives integrated either with <i>lux</i>ABCDE or <i>ilux2</i> operon. Eggs were imaged daily for bioluminescence detection, with dead embryos sampled immediately and survivors killed at 5 days post inoculation (dpi). The eggs were opened, and egg contents were imaged for bioluminescence. Yolks were sampled for <i>E. coli</i> isolation and quantification. Results showed lethality rates of 100%, 93.3% and 80% in embryos inoculated with native strain, <i>lux</i>ABCDE or <i>ilux2,</i> respectively. Bioluminescence analysis showed increased bioluminescence signal strength over time preceding embryo death. Surviving embryos exhibited a sequential reduction in signal strength. A strong positive correlation was found between bioluminescence signal intensity <i>in ovo</i> and <i>ex ovo</i>, with <i>ilux2-</i>APEC infected eggs showing a higher luminoscore than <i>lux</i>ABCDE-APEC. The <i>E. coli</i> load in yolks of APEC-inoculated eggs showed a positive trend over time. Overall, bioluminescence imaging of <i>ilux2</i> operon labelled bacteria enabled more efficient real-time detection and monitoring of <i>E. coli in ovo</i>. Multiple imaging sessions on the same embryo throughout the experiment allow precise monitoring of infection progression without sequential culling. This offers a controlled platform for evaluating antimicrobial treatments' efficacy in an <i>in ovo</i> model that closely resembles <i>in vivo</i> environment in chickens. It also bears the potential to study other pathogens' infection patterns, especially those pose risks to public health.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-24"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of highly pathogenic avian influenza virus (HPAIV) on Black-headed Gulls <i>Chroicocephalus ridibundus</i> population in Poland in 2023.","authors":"Piotr Indykiewicz, Marcin Przymencki, Piotr Minias, Dariusz Jakubas, Klaudia Litwiniak, Piotr Zieliński, Tomasz Janiszewski, Radosław Włodarczyk, Mateusz Ledwoń, Jacek Nowakowski, Beata Dulisz, Katarzyna Domańska-Blicharz, Edyta Świętoń, Krzysztof Śmietanka, Dariusz Bukaciński, Monika Bukacińska, Arkadiusz Buczyński, Szymon Beuch, Tomasz Chodkiewicz, Jacek Betleja, Szymon Bzoma, Dominik Marchowski, Arkadiusz Sikora, Łukasz Bednarz, Jacek Antczak, Andrzej Dylik, Zbigniew Kajzer, Wiesław Król, Łukasz Krajewski, Sebastian Menderski, Jan Rapczyński, Sławomir Rubacha, Rafał Szczerbik, Jakub Szymczak, Kazimierz Walasz, Przemysław Wylegała","doi":"10.1080/03079457.2025.2467122","DOIUrl":"https://doi.org/10.1080/03079457.2025.2467122","url":null,"abstract":"<p><p>The highly pathogenic avian influenza virus (HPAIV) A(H5N1) has caused the most extensive and severe epizootic event affecting both poultry and wild birds globally. This study investigated the impact of HPAIV on the breeding population of the Black-headed Gull <i>Chroicocephalus ridibundus</i>, the most abundant gull species in Poland. During the 2023 outbreak, this species was reported as the most frequently infected in the country. A higher-than-natural adult mortality rate (greater than 1.5% of the breeding individuals) was observed in 114 surveyed colonies across all regions of Poland. Laboratory tests confirmed the presence of HPAIV in all 17 colonies sampled, with average adult mortality estimated at 26.1%, and ranging from 1.7% to 77.8%. The estimated mortality rate across all surveyed colonies was 22.2%. Extrapolations across the entire Polish breeding population (at least 115,000 pairs according to the national census) indicated that approximately 51,000 adult Black-headed Gulls might have perished due to HPAIV in 2023. The number of adults found dead was positively correlated with colony size (<i>r</i> = 0.733, <i>P</i> < 0.001). The deaths were associated with a single HPAIV genotype (BB) across all confirmed cases. Understanding the spread and severity of HPAIV in colonially breeding waterbirds, such as gulls, is essential for assessing the full extent of the threats this virus poses to wild bird populations.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-14"},"PeriodicalIF":2.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of avian trypanosomes in domestic chickens (<i>Gallus gallus domesticus</i>) in Southeast Asia and review of the parasite morphometry in different avian hosts.","authors":"Pornchai Pornpanom, Gediminas Valkiūnas, Surya Paudel","doi":"10.1080/03079457.2025.2462628","DOIUrl":"10.1080/03079457.2025.2462628","url":null,"abstract":"<p><strong>Research highlights: </strong>The prevalence of trypanosomes in chickens is high.The first report of <i>Trypanosoma avium</i> in chickens with evidence of species identification.The first report of morphological and molecular characteristics of <i>Trypanosoma calmettei</i>.A cheap and quick buffy coat method is helpful for screening trypanosomes.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic analysis reveals the emergence and dissemination of different <i>Salmonella enterica</i> serovar Gallinarum biovar Gallinarum lineages in Brazil.","authors":"Diéssy Kipper, Renato Hohl Orsi, Nathalie de Souza Zanetti, Silvia De Carli, Andrea Karoline Mascitti, André Salvador Kazantzi Fonseca, Nilo Ikuta, Martin Wiedmann, Vagner Ricardo Lunge","doi":"10.1080/03079457.2025.2458601","DOIUrl":"10.1080/03079457.2025.2458601","url":null,"abstract":"<p><strong>Research highlights: </strong>Fowl typhoid (FT) is a concerning poultry disease caused by <i>S.</i> Gallinarum.Five <i>S.</i> Gallinarum lineages (I to V) were demonstrated in South American farms.<i>S.</i> Gallinarum lineages have specific antimicrobial resistance / virulence genomic profiles.Main FT outbreaks in Brazil have been caused by the specific lineage II.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological characteristics of keel bone maturation and keel bone fractures in laying hens.","authors":"Páll Gretarsson, Käthe Kittelsen, Randi Oppermann Moe, Ingrid Toftaker, Ida Thøfner","doi":"10.1080/03079457.2024.2448498","DOIUrl":"https://doi.org/10.1080/03079457.2024.2448498","url":null,"abstract":"<p><p>The keel bone in laying hens is prone to fractures, especially on the caudal third of the keel, which is also the last part to ossify. Keel bone fractures (KBF) typically occur between 25 and 50 weeks of age (WOA). However, the keel is fully ossified at around 40 WOA, suggesting fractures can occur before ossification is complete. To better understand the relationship between KBF and ossification, this descriptive study examined keel bone morphology during maturation. Keel bones from 50 commercial aviary housed Dekalb White laying hens were collected at 10 timepoints from 17-53 WOA and prepared for histological analysis. The samples were stained with haematoxylin and eosin and Safranin O to show cartilage, ossification, bone tissue, and KBF. The results indicated an ossification process similar to endochondral ossification. The degree of ossification varied between individuals of the same age. The age at complete ossification varied from 28-49 WOA. None of the keels from hens aged 53 WOA were fully ossified. Cartilage canals were present in the keel cartilage. Medullary bone was observed in all age groups. Most fractures lacked tissue morphologies typical of high-impact collisions, suggesting the need for further research into the underlying causes. This is the first study to detail keel bone histomorphology in commercial laying hens, providing baseline data for future research.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-16"},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and structural factors contributing to the dominance and persistence of goose astrovirus type 2.","authors":"Peng Jiang, Aolin Li, Hongfu Ma, Yankun Lan, Jiaguo Wang, Xin Wang, Liang Zhao, Qifei Wu, Yong Wang, Xu Guo","doi":"10.1080/03079457.2025.2454416","DOIUrl":"10.1080/03079457.2025.2454416","url":null,"abstract":"<p><strong>Research highlights: </strong>Goose astrovirus type 2 (GoAstV-2) became the dominant strain in China post-2017.GoAstV-2 exhibits weaker codon usage bias, enhancing adaptability across hosts.The stability of GoAstV-2 spike protein reduces mutation needs and selective pressure.GoAstV-2 low spike protein variability supports long-term persistence in host populations.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of <i>ttrA</i> and <i>pduA</i> genes in <i>Salmonella enterica</i> serovars induce a comparable immune response to wild-type infection in different chicken lineages.","authors":"Túlio Spina de Lima, Mauro de Mesquita Souza Saraiva, Adriana Maria de Almeida, Taisa Santiago Ferreira, Lucas Bocchini Rodrigues Alves, Viviane Amorim Ferreira, Oliveiro Caetano de Freitas Neto, Paul Andrew Barrow, Angelo Berchieri Junior","doi":"10.1080/03079457.2025.2457095","DOIUrl":"10.1080/03079457.2025.2457095","url":null,"abstract":"<p><strong>Research highlights: </strong><i>ttrA</i> and <i>pduA</i> double mutants in <i>Salmonella</i> provoke a similar immune response.SE elicited more intense immune responses than STM.The immune response in the broiler was more intense than in other lineages.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"1-12"},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity of duck circovirus and novel goose parvovirus co-infection in SPF ducks.","authors":"Yudong Zhu, Qiong Wu, Mian Wu, Dalin He, Bingrong Wu, Mingtian Mao, Wentao Tang, Jiake Li, Caiqi Wang, Hui Zhao, Yafei Qin, Youxiang Diao, Yi Tang","doi":"10.1080/03079457.2024.2383231","DOIUrl":"10.1080/03079457.2024.2383231","url":null,"abstract":"<p><p>Duck circovirus (DuCV) is one of the most prevalent infectious viruses in the duck industry in China. Although the clinical signs vary, it often causes immunosuppression in the host and leads to secondary infection with other pathogens. Novel goose parvovirus (NGPV) mainly infects ducks and causes short beak and dwarfism syndrome in ducks. However, the incidence of infection in ducks has increased in recent years, and the phenomenon of mixed infection with DuCV is common, resulting in more severe clinical morbidity. However, there are no systematic studies evaluating the presence of mixed infections. In order to investigate the synergistic pathogenicity of DuCV and NGPV co-infection in SPF ducks, a comparative experiment using DuCV and NGPV co-infection and mono-infection bird models was established. The results showed that the clinical signs of short beak, dwarfism and immunosuppression were more obvious in DuCV and NGPV co-infected ducks; the tissue damage of target organs was more serious, and the viral titre in organs and cloacal swabs were more significant compared with those of SPF ducks infected with only one virus. The results indicated that co-infection with DuCV and NGPV could promote viral replication and cause more severe tissue damage and immunosuppression than single virus infection. The present study reveals that the co-infection of NGPV and DuCV has a synergistic pathogenic effect from the aspect of pathogenicity, and the conclusions drawn not only clarify the direction of the subsequent research on the mechanism of co-infection of NGPV and DuCV, but also provide a scientific basis for the research on the co-infection of immunosuppressive pathogens and other pathogens.</p>","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":" ","pages":"76-82"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}