YbdO在调节禽致病性大肠杆菌耐酸性和生物膜形成中的作用。

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES

Avian Pathology Pub Date : 2025-06-16 DOI:10.1080/03079457.2025.2514210

Lumin Yu, Linan Xu, Yuzhong Zhao, Xiangpeng Yang, Junfei Ma, Bin Ye, Xinglin Zhang

引用次数: 0

摘要

禽致病性大肠杆菌（APEC）在禽类中引起被称为大肠杆菌病的严重系统性传染病，给全球家禽业造成重大经济损失，并威胁粮食安全和人类健康。先前的研究表明，耐酸是大肠杆菌在胃肠道中生存的不可或缺的机制；生物膜的形成导致宿主持续和反复感染，是抗菌药物难以治疗大肠杆菌病的主要原因。在本研究中，我们证实了YbdO是一种转录调节剂，通过上调生物膜相关基因bcsE、csgD、pgaA、wcaD、fimH和flhD的转录来增加生物膜的形成，并通过激活酸胁迫伴侣HdeA和酸胁迫反应蛋白YqgB的表达来增强APEC CE1的耐酸性。此外，电泳迁移率转移实验（EMSA）表明，YbdO通过直接结合CsgD、HdeA和YqgB的启动子，直接激活了CsgD、HdeA和YqgB的表达。这些发现证实了YbdO对耐酸性和生物膜形成的调控机制，并加深了我们对APEC如何提高对环境胁迫的适应性，从而提高APEC致病性的认识。因此，该研究可能为生物膜形成和耐酸性的调控提供一个范例，可用于研究其他细菌病原体。

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The role of YbdO in regulating acid resistance and biofilm formation in avian pathogenic Escherichia coli.

Research highlights: YbdO increases biofilm formation and acid resistance.YbdO upregulates the transcription levels of the biofilm-associated genes bcsE, csgD, pgaA, wcaD, fimH, and flhD.YbdO activates the expression of acid stress-associated genes hdeA and yqgB.YbdO contributes to improving the adaptability of APEC to environmental stresses.

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来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.