Ana Colom-Rivero, Antonio Fernández, Lucía Marrero-Ponce, Ayoze Castro-Alonso, Candela Rivero-Herrera, Lucía Caballero-Hernández, Cristian Suárez-Santana, Eva Sierra
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引用次数: 0
AbstractAvian herpesviruses (AHVs) are widely distributed and associated with a variety of diseases affecting bird populations globally. Despite the increasing detection of AHVs in recent years, there remains a significant gap in knowledge regarding their classification and host range. This study aimed to detect herpesvirus (HV) in two vulnerable, endemic subspecies of the stone-curlews (Burhinus oedicnemus) in the Canary Islands. Forty-six pooled tissue swabs (liver, kidney, and lung) and 135 individual swaps (brain, cloaca, and oropharyngeal cavity) were collected from 50 stone-curlews recorded as deceased wildlife specimens between 2020 and 2023. DNA from a novel alpha-HV was successfully amplified from 7 out of the 181 tissue samples (4%) and from 4 out of 50 birds analyzed (8%) using a semi-nested polymerase chain reaction (PCR) approach with degenerate primers. Positive samples were distributed across various tissue types: brain (n = 1), kidney (n = 1), lung (n = 2), coelomic cavity (n = 1), and oropharyngeal swab (n = 2). Some individuals tested positive in multiple tissue types, although no histopathological features indicative of HV infection were observed in any of the animals. Sequencing of all positive samples revealed identical HV nucleotide sequences across all specimens. The longest PCR amplicon, obtained with the TGV and KG1 primer combination, yielded identical sequences in two of the seven positive samples. Based on these findings, we propose the designation of this novel HV as Burhinus oedicnemus alphaherpesvirus.
期刊介绍：
Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.
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