Recombinant auto-bioluminescent Escherichia coli to monitor the progression of Escherichia coli infection in the embryonated chicken eggs.

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES

Avian Pathology Pub Date : 2025-04-24 DOI:10.1080/03079457.2025.2477246

Mohamed Kamal Abdelhamid, Surya Paudel, Hammad Ur Rehman, Manolis Lyrakis, Ivana Bilic, Michael Hess, Claudia Hess

引用次数: 0

Abstract

Research highlights: Bioluminescence imaging enabled real-time, noninvasive tracking of a bioluminescent APEC infection in embryonated chicken eggs over time.Bioluminescence signals showed contrasting patterns for dead and surviving embryos.The ilux2-APEC showed a higher luminoscore than luxABCDE-APEC in inoculated embryonated chicken eggs.The in ovo bioluminescent signal from intact eggs effectively reflects the ex ovo signal following the take out of yolk and embryo.

查看原文本刊更多论文

重组自体发光大肠杆菌监测鸡胚中大肠杆菌感染的进展。

家禽致病性大肠杆菌（APEC）感染对健康和生产产生不利影响，具有公共卫生意义。本研究评估了生物发光成像在实时、无创跟踪12日龄鸡胚胎中微生物进展的潜力，这些胚胎接种了含有luxABCDE或ilux2操纵子的APEC菌株或其衍生物。每天对卵子成像进行生物发光检测，立即采集死亡胚胎，接种后5天杀死存活胚胎（dpi）。将卵打开，对卵内内容物进行生物发光成像。取蛋黄进行大肠杆菌的分离和定量。结果表明，本地菌株、luxABCDE和ilux2接种胚胎的致死率分别为100%、93.3%和80%。生物发光分析显示，随着胚胎死亡时间的推移，生物发光信号强度增加。存活的胚胎表现出信号强度的连续下降。卵和卵外的生物发光信号强度之间存在很强的正相关，ilux2-APEC感染的卵的发光评分高于luxABCDE-APEC。随着时间的推移，接种apec的鸡蛋蛋黄中的大肠杆菌载量呈阳性趋势。总的来说，ilux2操纵子标记细菌的生物发光成像能够更有效地实时检测和监测鸡蛋中的大肠杆菌。在整个实验过程中，对同一个胚胎进行多次成像，可以精确监测感染进展，而无需顺序剔除。这为在蛋内模型中评估抗菌治疗的效果提供了一个可控的平台，该模型与鸡体内环境非常相似。它还具有研究其他病原体感染模式的潜力，特别是那些对公共卫生构成风险的病原体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.