European Journal of Soil Biology最新文献

{"title":"Soil extracellular enzyme stoichiometry reveals the nutrient limitations of soil microbial metabolism under precipitation changes in Ningxia desert steppe of China","authors":"Jiali Lian ,&nbsp;Jing Chen ,&nbsp;Cui Han ,&nbsp;Ying Zhao ,&nbsp;Xueqin Yang ,&nbsp;Jianping Li","doi":"10.1016/j.ejsobi.2025.103774","DOIUrl":"10.1016/j.ejsobi.2025.103774","url":null,"abstract":"<div><div>Soil microbial communities and extracellular enzyme activity in arid ecosystems are highly sensitive to precipitation changes, yet their metabolic responses remain poorly understood. Through a field precipitation experiment in the Ningxia's desert steppe, we found that increased precipitation significantly enhanced C-, N-, and P-acquiring enzyme activities, with extracellular enzyme stoichiometry revealing microbial P limitation. Soil microbial communities were dominated by the phyla <em>Actinobacteriota</em>, <em>Chloroflexi</em>, and <em>Proteobacteria</em> (bacteria) and <em>Ascomycota</em> (fungi) under altered precipitation. Structural equation modeling (SEM) revealed that biotic factors (community structure/diversity) exerted stronger control over metabolic limitations than abiotic factors, with P limitation surpassing C limitation. These findings highlight P availability as a critical constraint on microbial function in arid grasslands. Our study provides actionable insights for grassland restoration, suggesting targeted P fertilization could mitigate microbial nutrient limitations and enhance ecosystem resilience under climate change.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103774"},"PeriodicalIF":3.3,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Legume integration into rice cropping systems buffers topsoil functional potential against microbial diversity loss","authors":"Bin Zhang ,&nbsp;Zhanbo Wei ,&nbsp;Rui Zhu ,&nbsp;Evgenios Agathokleous ,&nbsp;Jiacheng Zhao ,&nbsp;Eiko E. Kuramae","doi":"10.1016/j.ejsobi.2025.103775","DOIUrl":"10.1016/j.ejsobi.2025.103775","url":null,"abstract":"<div><div>Intensive cropping systems pose a growing threat to soil microbial diversity, potentially impairing essential agroecosystem functions. Introducing legume crops or implementing fallow periods into these systems are promising strategies to alleviate such negative impacts. However, how these strategies affect the resilience of soil functions to microbial diversity loss remains largely unexplored, particularly in deeper soil layers. In this study, we employed a dilution-to-extinction approach to simulate microbial diversity loss and investigated its effect on functional potential in both topsoil (0–20 cm) and subsoil (40–60 cm) under three crop rotation systems (i.e., rice-fallow, rice-wheat, rice-milk vetch). Soil functional potential was indicated by measuring the copy number of functional genes using high-throughput qPCR. Our results indicate that microbial diversity loss significantly reduced abundance of genes associated with C degradation, C fixation, N mineralization, nitrification, and denitrification in the topsoil of rice-fallow and rice-wheat systems. In contrast, the rice-milk vetch system preserved abundance of these functional genes in the topsoil following microbial diversity loss, highlighting the potential of tailored cropping strategies to counteract the adverse effect of intensive agriculture. Furthermore, while abundance of genes associated with nitrification was also reduced in subsoil by microbial diversity loss, that of genes associated with C degradation and denitrification generally increased for all cropping systems. This highlights the vulnerability of subsoil function potential to microbial diversity loss, potentially enhancing greenhouse gas emissions and contributing to positive climate feedbacks. We concluded that integrating legume crops can maintain soil functional potential in topsoil even in the face of reduced microbial diversity, which is crucial for developing sustainable agricultural practices and ensuring long-term agroecosystem resilience.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103775"},"PeriodicalIF":3.3,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial mechanism of soil organic carbon content under waterlogging and water table fluctuation in drained peat soils","authors":"Xiaohan Li ,&nbsp;Yang Wang ,&nbsp;Jiye Cai ,&nbsp;Guangyuan Xu ,&nbsp;Hongkai Li ,&nbsp;Yanmin Dong ,&nbsp;Shasha Liu ,&nbsp;Ziping Liu ,&nbsp;Shengzhong Wang ,&nbsp;Zhiwei Xu","doi":"10.1016/j.ejsobi.2025.103773","DOIUrl":"10.1016/j.ejsobi.2025.103773","url":null,"abstract":"<div><div>Peatlands serve as crucial reservoirs within the global carbon cycle; however, widespread degradation has led to significant reductions in soil organic carbon (SOC) contents. The effects of rewetting on the active SOC and the underlying microbial mechanisms in degraded peatlands are still not fully elucidated. This study examined changes in bulk and active SOC contents, as well as microbial community composition and enzyme activities via controlled water table (WT) manipulation in intact peat columns sourced from drained sites in the Changbai Mountains. The results demonstrated that variations in WT significantly influenced microbial biomass and enzymatic activities within the surface soil layer. Waterlogging resulted in decreased total bacterial and actinomycete biomass, accompanied by a marked increase in fungal biomass, especially under WT fluctuation. Soil oxidase and hydrolase activities only increased significantly under WT fluctuation treatments. SOC contents rose under waterlogged conditions but declined with WT fluctuation. Both dissolved organic carbon (DOC) and microbial biomass carbon were elevated in surface soils subjected to waterlogged and WT fluctuation compared to those in drained peat. However, the easily oxidizable carbon contents were only elevated in surface soils subjected to waterlogged treatments compared to those in drained peat. Soil moisture content had the greatest positive influence, while pH had the greatest negative influence on SOC contents. Actinomycete and DOC had negative impacts on SOC contents under varied WT conditions. This study clarifies the regulatory influence of WT on microbial community dynamics and enzyme activities relevant to SOC storage in degraded peatlands, highlighting the importance of WT management for effective peatland restoration.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"127 ","pages":"Article 103773"},"PeriodicalIF":3.3,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplanting Phoebe bournei modifies soil microbial community characteristics in Cunninghamia lanceolata monocultures","authors":"Dan Zhou ,&nbsp;Chengjian Hong ,&nbsp;Jiahuan Guo ,&nbsp;Chang Pan ,&nbsp;Yazhou Tang ,&nbsp;Jie Yan ,&nbsp;Kaizhi Xie ,&nbsp;Yuanchun Yu","doi":"10.1016/j.ejsobi.2025.103762","DOIUrl":"10.1016/j.ejsobi.2025.103762","url":null,"abstract":"<div><div>The prolonged monoculture of <em>Cunninghamia lanceolata</em> depletes soil fertility, making the introduction of broadleaf trees—<em>Phoebe bournei</em> indispensable for soil restoration—yet its impact on the belowground ecological environment remains inadequately explored. Here, we investigated how uneven-aged interplanting of <em>P. bournei</em> in subtropical <em>C. lanceolata</em> stands alters microbial community characteristics and correlates with major edaphic variables across 0–60 cm soil profile. Interplanting <em>P. bournei</em> improved soil aeration, water conservation, and nutrient availability, driving significant shifts in bacterial and fungal β-diversity and partial but significant changes in community composition. It also enhanced ecological drift and reduced dispersal limitation, strengthening homogeneous selection in bacterial communities (0–40 cm) while promoting drift in fungal communities (0–60 cm). These structural and assembly changes suggest potential functional alterations in organic-matter degradation and nutrient cycling. Interplanting <em>P. bournei</em> led to marked improvements in soil microbial ecology, as evidenced by higher abundances of Acidobacteria and Ascomycota, indicative of enhanced complex-carbon degradation; elevated bacterial cellulolytic capacity and accelerated organic-matter turnover; promotion of saprotroph–symbiotroph nutrition; and reduced subsoil pathotroph abundance, which may indirectly support <em>C. lanceolata</em> health. Lastly, soil nutrient elements (e.g., soil organic carbon, total phosphorus) and enzyme activities (e.g., Cellobiohydrolase, Sucrase) were identified as key drivers of microbial community structure and functional potential, highlighting their critical roles in shaping soil microbial ecosystems. In summary, uneven-aged interplanting <em>P. bournei</em> in <em>C. lanceolata</em> plantations optimized soil ecosystem functions, offering a sustainable strategy to enhance forest productivity and improve soil health.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103762"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of furrow-applied biochar in enhancing rhizosphere soil microbiota and metabolites in continuous sesame cropping","authors":"Ruiqing Wang ,&nbsp;Fengjuan Lyu ,&nbsp;Rujie Lyu ,&nbsp;Junhai He ,&nbsp;Lingen Wei","doi":"10.1016/j.ejsobi.2025.103763","DOIUrl":"10.1016/j.ejsobi.2025.103763","url":null,"abstract":"<div><div>Biochar application significantly reduces bacterial wilt and boosts yield in continuous sesame cropping, but high costs limit its widespread use. This study used metagenomic and metabolomic analyses to evaluate three biochar application strategies (surface [BR3, 15,000 kg ha⁻¹], furrow [BR4, 1,875 kg ha⁻¹], and no biochar [BR0]) across four sample types (D0/D1: diseased; H0/H1: healthy; rhizosphere/non-rhizosphere), analyzing their effects on microbial community as well as their functional and metabolic profiles in sesame soil. The results showed that BR4 significantly increased sesame yield by 26.57 % compared to the control (BR0). Biochar treatments (BR3/BR4) enhanced the relative abundance of <em>Actinomycetota</em> while suppressing <em>Pseudomonas</em> in rhizosphere and non-rhizosphere soils of diseased plants. <em>Sphingobium amiense</em> and <em>S. indicum</em> were differentially abundant in BR3H0 and BR4H0, respectively. We observed disruption of fatty acid metabolism in BR3D0, which was linked to growth inhibition in continuously cropped sesame. BR4H0 treatment significantly increased the relative abundance of pyruvate dehydrogenase and the superpathway for de novo biosynthesis of pyrimidine ribonucleotides. The rhizospheres treated with BR4 from healthy plants showed higher levels of C17-sphinganine (+2.79 percentage points) and 6-[3-(dihydroxyphenyl-methyl)-methoxybutyl]-benzodioxole (+0.18 percentage points) compared to diseased plants. Partial Least Squares Discriminant Analysis (PLS-DA) identified Jasmine lactone and Tris(hydroxymethyl)aminomethane as key metabolite discriminators under positive ion mode, as opposed to Taurochenoxycholate and Palmitic acid under negative ion mode. These findings suggest that furrow-applied biochar at a reduced rate (1,875 kg ha⁻¹) can enhance soil microbial structure, function, and metabolite composition, ultimately improving yield in continuous sesame cropping systems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103763"},"PeriodicalIF":3.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating soil trophic links in a peatland, northeast China: Dual stable isotope analysis (δ13C and δ15N) of microarthropods and their food sources","authors":"Dandan Liu ,&nbsp;Yiling Lin ,&nbsp;Haitao Wu","doi":"10.1016/j.ejsobi.2025.103761","DOIUrl":"10.1016/j.ejsobi.2025.103761","url":null,"abstract":"<div><div>Peatlands are crucial global carbon sinks and key players in carbon cycles, with their soil food webs supporting vital ecosystem processes. However, trophic relationships and carbon source uses of soil microarthropods in peatlands are poorly understood, limiting our understanding of their role in material cycling and ecosystem stability. Here, we employed dual stable isotope analysis (δ¹³C and δ¹⁵N) to investigate the trophic structure and carbon source utilization of soil microarthropods (Collembola, Oribatida, and Mesostigmata) in the Hani Peatland of northeastern China. Our results reveal clear trophic differentiation among taxa, with Mesostigmata occupying higher positions and Oribatida displaying broader isotopic niches. Although Collembola exhibited a weak correlation between body mass and isotope values, this pattern was not consistent across taxa. Mosses, particularly <em>Polytrichum strictum</em>, contributed significantly to the carbon intake of Collembola and Oribatida. However, δ¹³C enrichment patterns suggest that carbon transfer is primarily mediated through moss-associated microbial pathways rather than direct moss consumption. These findings provide novel insights into the structure and functioning of peatland soil food webs and highlight the ecological importance of microarthropods in belowground biogeochemical cycling. Understanding their roles is critical for predicting peatland responses to environmental change and informing conservation strategies.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103761"},"PeriodicalIF":3.3,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crop rotation and organic fertilizer maintains diversity and activity of cbbL-carrying CO2-fixing bacteria in reclaimed coal mining soils","authors":"Shuning Bai ,&nbsp;Meihua Fan ,&nbsp;Min Wu ,&nbsp;Xiaolong Sui ,&nbsp;Yibo Song ,&nbsp;Yunlong Jiang ,&nbsp;Huisheng Meng ,&nbsp;Yulin Liu ,&nbsp;Xiangying Wang ,&nbsp;Xianjun Hao ,&nbsp;Yi Li ,&nbsp;Jianping Hong ,&nbsp;Jie Zhang","doi":"10.1016/j.ejsobi.2025.103759","DOIUrl":"10.1016/j.ejsobi.2025.103759","url":null,"abstract":"<div><div>CO₂-fixing bacteria are an important factor in restoring soil health in coal mining areas. The impact of crop rotation and fertilization on CO₂-fixing bacteria in reclaimed mining soils remains unclear. To narrow this knowledge gap, in this study, maize (<em>Zea mays</em> L.) monoculture (M) and maize-soybean (<em>Glycine max</em>) rotation (R) cropping systems were set up in a coal-mining reclamation area with four fertilization treatments in each, namely, CK (without fertilization), inorganic fertilizer (F), organic fertilizer (O), and combined organic and inorganic fertilizer (OF). The abundance, diversity, community composition and RubisCO activity of CO₂-fixing bacteria in topsoil under those treatments were investigated respectively using quantitative PCR, high-throughput sequencing based on the <em>cbbL</em> gene [that encodes ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO)] and enzyme-linked immunosorbent assay (ELISA). The results showed that R_O significantly increased easily oxidized organic carbon (EOC), total nitrogen (TN), and available nitrogen (AN) (<em>P</em> &lt; 0.05). It also significantly increased the biomass of CO₂-fixing bacteria (<em>P</em> &lt; 0.05) and altered the CO₂-fixing bacterial community. The CO₂-fixing bacteria in R_OF, R_O and M_O exhibited comparable community structures and harbored a greater co-occurrence network complexity than other treatments. Several CO₂-fixing bacteria associated with nitrogen cycling, such as <em>Devosia</em>, <em>Nitrobacter</em>, Hyphomicrobiales and <em>Nitrosospira</em>, were significantly enriched under the maize-soybean rotation system (<em>P</em> &lt; 0.05). This study implied that crop rotation and organic fertilizer application could synergistically foster soil quality restoration in coal mining area by elevating soil nutrients and maintaining biomass, diversity and community structure of <em>cbbL</em>-carrying CO₂-fixing bacteria, establishing a theoretical foundation for optimizing carbon sequestration strategies in post-mining ecological rehabilitation.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103759"},"PeriodicalIF":3.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional endophytic bacteria intimately associated within spores of arbuscular mycorrhizal fungi in a chernozem soil in Central Europe","authors":"Helmut Baltruschat ,&nbsp;Johanna Hummel ,&nbsp;Marit Gillmeister ,&nbsp;Stefan Ratering ,&nbsp;Kathrin Kabrodt ,&nbsp;Ewald Sieverding ,&nbsp;Fritz Oehl","doi":"10.1016/j.ejsobi.2025.103760","DOIUrl":"10.1016/j.ejsobi.2025.103760","url":null,"abstract":"<div><div>Chernozems are counted among the most fertile soils worldwide. Unexpectedly high spore density and species richness of arbuscular mycorrhizal fungi (AMF) were found in a long-term field trial established on such a soil. The purpose of the present study was to estimate bacterial communities associated within spores of selected AMF species from a long-term field trial on a highly fertile Calcic Chernozem to unravel their diversity belonging to different genera and species. We hypothized that high AMF species richness found in the Chernozem soil is reflected in a bacterial diversity with multifunctional traits mediated by indigenous bacterial compositions. The AMF species <em>Funneliformis mosseae</em>, <em>Scutellospora calospora</em> and <em>Septoglomus nigrum</em> were selected, since they occurred abundantly both in reduced and conventional tillage systems. The pure cultures of isolated bacterial strains were tested for ecological functions (traits) such as phosphorus solubilization, siderophore production, indole-3-acetic acid production and 1-aminocyclopropane-1-carboxylate deaminase activity. In addition, antimicrobial activity against both hemibiotrophic and necrotrophic fungi and oomycetes was evaluated. The majority of bacterial strains was exclusively associated with only one of the three AMF species, thus, giving evidence that each AMF species may harbor its own bacterial community. A large number of bacterial communities was shown to exert multifunctional activities ranging from plant growth promotion traits to antimicrobial activity. These findings suggest that the multifunctionality of bacteria intimately associated with AMF could markedly expand the ecological function of an autochthonous AMF population and empower host plants to explore robust ways to cope with changing environmental conditions.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103760"},"PeriodicalIF":3.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ant-isopod interactions: spanning predation, facilitation, and myrmecophily","authors":"Thomas Parmentier ,&nbsp;Stefano Taiti ,&nbsp;Pallieter De Smedt","doi":"10.1016/j.ejsobi.2025.103753","DOIUrl":"10.1016/j.ejsobi.2025.103753","url":null,"abstract":"<div><div>Ants and terrestrial isopods are two key groups within soil ecosystems both in terms of their biomass and the vital functions they contribute to. Despite the ecological importance of both groups and their frequent interactions, the dynamics between ants and terrestrial isopods remain poorly understood. We provide a systematic overview and discuss the diverse spectrum of ant-isopod interactions ranging from predation, through facilitation, to myrmecophily.</div><div>A limited number of ant species, restricted to the genus <em>Leptogenys</em>, specialize in preying on isopods, while a very broad group of ants opportunistically include them in their diet. To evade ant predation, terrestrial isopods employ defensive strategies such as avoidance, conglobation or secretion of repellent substances. Apart from the direct negative impacts of predation, ants impose several non-lethal effects on terrestrial isopods, influencing their spatial distribution, behaviour, and fitness. Invasive ants may, in contrast, indirectly benefit isopods by feeding on their predators or competitors. A diverse group of isopods are not deterred by ants and reside in their nests. These myrmecophilous isopods vary from facultative associates, which cohabit with ants only occasionally and are represented by many records, to obligate guests that live permanently within ant nests. These obligate guests thrive in ant fortresses by specific behavioural, chemical and morphological adaptations. Their effect on the host can range from negative to neutral, or potentially even beneficial. Though modest in number, with 23 species across 10 genera, the diversity of obligate myrmecophilous isopods showcases a fascinating pattern of independent evolution in soil ecosystems.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103753"},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the microbial mechanisms underlying glucose induced soil priming effects under low and high nutrient levels","authors":"Jiaqi Wang ,&nbsp;Xichang Zhang ,&nbsp;Yingyi Fu ,&nbsp;Lukas Van Zwieten ,&nbsp;Han Sun ,&nbsp;Georg Guggenberger ,&nbsp;Lingfei Hu ,&nbsp;Yu Luo ,&nbsp;Tida Ge ,&nbsp;Yakov Kuzyakov","doi":"10.1016/j.ejsobi.2025.103757","DOIUrl":"10.1016/j.ejsobi.2025.103757","url":null,"abstract":"<div><div>Soil priming effect, which refers to the impact of labile carbon inputs on the decomposition of soil organic matter, plays a significant role in carbon storage. Investigating substrate induced soil priming effects and the involved microbial mechanisms, particularly under nutrients gradient, is central to the understanding of carbon processes and potential accrual in agriculture soil. Thus, a 7 days laboratory incubation was conducted to assess ¹³C labeled glucose induced priming effects in soil receiving the high (TH) and low nutrient (TL) addition. Also, DNA-SIP coupled with metagenomic were adopted to identify the core microbial groups and functional guild responsible for soil priming effects. Here, we found that i) soil priming effects were significantly larger in TL treatment than in the TH treatment, and ii) the larger priming in TL was likely driven by N-mining processes dominated by K-strategy microbes, whereas less priming in TH might be explained by co-metabolism led by r-strategy microbes. Additionally, functional changes of microbial community were revealed by Shotgun sequencing. Both KEGG, EggNOG and CAZymes showed the relative abundance of the functional genes (e.g., GH13_10 and GH77) encoding cellulase enzymes involved in soil organic carbon decomposition were more abundant in TL compared to TH, suggesting higher priming effects in TL was mainly due to the nutrient constraints on microbial demands. This study revealed the main microbial groups and their functions in glucose induced soil priming effects under low and high nutrient levels.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"126 ","pages":"Article 103757"},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}