Journal of Agricultural and Food Chemistry最新文献_第8页

Unveiling the Structural and Biochemical Characteristics of an Acidophilic β-Mannanase from Soybean (Glycine max) 大豆亲酸性β-甘露聚糖酶（Glycine max）的结构及生化特性研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c03141

Chun-Jung Lin, Chao-Cheng Cho, Sheng-Chia Chen, Gloria Meng-Hsuan Lin, Cheng-Yang Huang, Chun-Hua Hsu

{"title":"Unveiling the Structural and Biochemical Characteristics of an Acidophilic β-Mannanase from Soybean (Glycine max)","authors":"Chun-Jung Lin, Chao-Cheng Cho, Sheng-Chia Chen, Gloria Meng-Hsuan Lin, Cheng-Yang Huang, Chun-Hua Hsu","doi":"10.1021/acs.jafc.5c03141","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03141","url":null,"abstract":"Endo-1,4-β-mannanase (EC 3.2.1.78) cleaves β-1,4-mannans in cell walls, facilitating endosperm softening and seed germination. Here, we present the structural and biochemical characterization of GmMAN19-1, a GH5_7 β-mannanase from soybean (Glycine max), a crop with substantial agricultural importance. GmMAN19-1 is specifically expressed in cotyledons during postgermination and exhibits acidophilic activity optimal at pH 4.6 and 40 °C. Crystal structures of GmMAN19-1 were determined at 1.39 and 2.62 Å in the apo form and in complex with mannopentaose (M5), respectively. The structure adopts a canonical (α/β)8 TIM barrel fold with a V-shaped active site groove. Notably, two distinct M5 binding modes were identified, suggesting dual functionality involving hydrolytic and transglycosylation activities. Site-directed mutagenesis further validated key catalytic and substrate-interacting residues: E186A abolished enzymatic activity, while Q267W altered transglycosylation product profiles and enhanced activity toward branched substrates. The binding groove can accommodate galactose side chains, supporting partial activity toward galactomannans. These findings provide comprehensive insights into the substrate specificity and catalytic mechanism of plant β-mannanases and establish GmMAN19-1 as a potential candidate for applications in food processing, biomass conversion, and industrial biotechnology, particularly due to its acidophilic nature and enhanced activity toward branched mannans.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"28 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Malonylation Enhances the Neuroprotection of Cyanidin-3-O-Glucoside by Promoting Gut-Derived 5-Hydroxytryptophan Production via Limosilactobacillus reuteri through the Gut-Brain Axis. 多重丙二醛化通过促进肠道来源的5-羟色氨酸通过肠-脑轴通过罗伊氏乳酸杆菌产生，增强了花青素-3- o -葡萄糖苷的神经保护作用。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c09840

Junjie Li,Guijie Chen,Tao Zhang,Guoyuan Xiong,Chuanlai Du,Yujia Peng,Xiaoxiong Zeng,Chunxu Chen

{"title":"Multi-Malonylation Enhances the Neuroprotection of Cyanidin-3-O-Glucoside by Promoting Gut-Derived 5-Hydroxytryptophan Production via Limosilactobacillus reuteri through the Gut-Brain Axis.","authors":"Junjie Li,Guijie Chen,Tao Zhang,Guoyuan Xiong,Chuanlai Du,Yujia Peng,Xiaoxiong Zeng,Chunxu Chen","doi":"10.1021/acs.jafc.5c09840","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09840","url":null,"abstract":"This study synthesized and structurally characterized malonylated derivatives of cyanidin-3-O-glucoside (C3G), namely cyanidin-3-O-(6-O-malonyl-β-d-glucoside) (C3GM) and cyanidin-3-O-(3,6-O-dimalonyl-β-d-glucoside) (C3GdM), and evaluated their neuroprotective effects in a high-fructose diet (HFrD)-induced neuroinflammation mouse. Malonylation enhanced C3G's neuroprotective bioactivity in a dose-dependent manner, with C3GdM showing superior efficacy in improving cognitive impairment, suppressing neuroinflammatory responses, restoring gut barrier integrity, and reshaping gut microbiota composition, notably enriching Lactobacillus. The key strain Limosilactobacillus reuteri LJJ 240337 was isolated and shown to synergize with C3GdM metabolism to produce gut-derived 5-hydroxytryptophan (5-HTP), a pivotal metabolite mediating neuroprotection against inflammation, validated in germ-free mice. Transcriptomic analysis further confirmed its conversion to 5-hydroxytryptamine (5-HT) in the brain, activating the cAMP/PKA/CREB signaling pathway, thereby contributing to the neuroprotective effects. These findings establish a potential structure-activity relationship between malonylation degree and neuroprotective efficacy. They also underscore the therapeutic potential of multiacylated anthocyanins in modulating the gut-brain axis and mitigating metabolic neuroinflammation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"51 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piceatannol Attenuates Benzo[a]pyrene/DSS-Induced Colorectal Cancer in Mice via Modulation of Gut Microbiota and Inhibition of the PI3K/AKT/mTOR Pathway picetanol通过调节肠道菌群和抑制PI3K/AKT/mTOR通路减轻苯并[a]芘/ dss诱导的小鼠结直肠癌

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c05807

Pin-Yu Ho, Yen-Chun Koh, Wei-Sheng Lin, Chin-Jui Ho, Anju Majeed, Chi-Tang Ho, Min-Hsiung Pan

{"title":"Piceatannol Attenuates Benzo[a]pyrene/DSS-Induced Colorectal Cancer in Mice via Modulation of Gut Microbiota and Inhibition of the PI3K/AKT/mTOR Pathway","authors":"Pin-Yu Ho, Yen-Chun Koh, Wei-Sheng Lin, Chin-Jui Ho, Anju Majeed, Chi-Tang Ho, Min-Hsiung Pan","doi":"10.1021/acs.jafc.5c05807","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05807","url":null,"abstract":"Benzo[a]pyrene (B[a]P) promotes colorectal cancer (CRC) under chronic inflammation. Piceatannol (PIC), a polyphenol with anti-inflammatory properties, was investigated for its timing-dependent effects in a B[a]P/dextran sulfate sodium (DSS)-induced CRC mouse model. ICR mice received B[a]P and DSS, followed by PIC intervention at different time points. Continuous PIC treatment (PI group) significantly reduced tumor burden, disease activity index, and intestinal permeability. Proinflammatory cytokines significantly decreased, whereas interleukin-10 increased. RNA-seq analysis showed that PIC upregulated transglutaminase 3 (Tgm3), which was associated with suppression of the phosphoinositide 3-kinase/AKT/mechanistic target of rapamycin pathway, downregulation of proinflammatory and tumor-promoting genes, and enhancement of epithelial repair genes. Gut microbiota analysis demonstrated restored microbial diversity, characterized by increased Roseburia faecis and Kineothrix alysoides and a reduction in Turicibacter sanguinis and Romboutsia ilealis. Continuous piceatannol administration attenuates CRC through microbiota and inflammatory regulation, with Tgm3 upregulation potentially contributing to these effects.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"57 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytic Mechanism and Engineering of Hydroxysteroid Dehydrogenases in the Biosynthesis of Ursodeoxycholic Acid. 羟基类固醇脱氢酶在熊去氧胆酸生物合成中的催化机理及工程研究。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c09495

Jiayi Feng,Jiamin Xue,Xuechao Hu,Luchao Ren,Lujing Ren

引用次数: 0

Construction of a Sensing Platform Integrated with a CRISPR/Cas12a-Triggered Colorimetric Strategy for the Quantitative Detection of Meat Freshness. 基于CRISPR/ cas12a触发比色策略的肉类新鲜度定量检测传感平台构建

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c04851

Junsheng Sheng,Yang Dong,Siyu Sun,Yanbin Zhang,Changhe Li,Xinglian Xu,Huhu Wang

{"title":"Construction of a Sensing Platform Integrated with a CRISPR/Cas12a-Triggered Colorimetric Strategy for the Quantitative Detection of Meat Freshness.","authors":"Junsheng Sheng,Yang Dong,Siyu Sun,Yanbin Zhang,Changhe Li,Xinglian Xu,Huhu Wang","doi":"10.1021/acs.jafc.5c04851","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04851","url":null,"abstract":"Monitoring microbial determinants, such as Pseudomonas spp., is thus essential for assessing meat freshness. Here, a novel colorimetric sensing platform based on magnetic enzyme-labeled nanoparticles combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a without nucleic acid molecule preamplification was developed for detecting meat freshness. Under optimal conditions, a high-specificity crRNA was systematically verified, and the colorimetric sensor could accurately quantify Pseudomonas spp. loads with levels ranging from 1 × 103.7 to 1 × 108.7 CFU/mL, with a color change from colorless to yellow. A smart colorimetric platform, including a self-designed image acquisition device and self-programmed image analysis software, was developed and applied to the integrated determination of meat freshness by using the B-value in the RGB channel. The platform has been applied to both consumers and producers and has been validated by 48 actual samples of chilled meat. These findings provide new insights into the exploration of reliable tools for monitoring meat freshness.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Impacts of Conventional and Biodegradable Microplastics on Cadmium Transfer in a Soil-Earthworm-Lettuce System. 常规和可生物降解微塑料对土壤-蚯蚓-生菜系统中镉迁移的差异影响。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c08866

Li Chen,Nan Chang,Changchao Li,Tao Li,Tianyi Qiu,Qingliang Cui,Haoran He,Fengyu Huang,Haijian Bing,Jason C White,Linchuan Fang

{"title":"Differential Impacts of Conventional and Biodegradable Microplastics on Cadmium Transfer in a Soil-Earthworm-Lettuce System.","authors":"Li Chen,Nan Chang,Changchao Li,Tao Li,Tianyi Qiu,Qingliang Cui,Haoran He,Fengyu Huang,Haijian Bing,Jason C White,Linchuan Fang","doi":"10.1021/acs.jafc.5c08866","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08866","url":null,"abstract":"Microplastics (MPs) and cadmium (Cd) are widespread agricultural contaminants, yet the interactions of MPs with Cd in agroecosystems are unknown. Here, we conducted a microcosm experiment to investigate the impacts of different MP types, including conventional (CMPs) and biodegradable MPs (BMPs), on the transfer of Cd in a soil-earthworm-lettuce system. The high dose (2%) of CMPs significantly increased the Cd concentration in plant shoots and earthworms by 54.1% and 79.9%, respectively. However, high-dose BMP exposure decreased the shoot Cd concentration by 30.3%. This could be explained by significant changes in soil properties, available Cd, and microbial communities after CMP and BMP exposure. Metabolomic analysis revealed that the CMP was significantly more enriched than the BMP group in metabolic pathways related to organic acid biosynthesis, which may increase soil Cd availability. Our study provides important insights for understanding the potential risk of Cd contamination of the food chain under MP exposure.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Casein-Derived Immunomodulatory Peptides Maintaining Immune Homeostasis via Regulating Gut Microbiota in a Cyclophosphamide-Induced Mouse Model 酪蛋白来源的免疫调节肽通过调节肠道微生物群在环磷酰胺诱导小鼠模型中维持免疫稳态的机制

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c07986

Siyi Li, Zichen Li, Yanfeng Tuo, Guangqing Mu, Shujuan Jiang

{"title":"Mechanisms of Casein-Derived Immunomodulatory Peptides Maintaining Immune Homeostasis via Regulating Gut Microbiota in a Cyclophosphamide-Induced Mouse Model","authors":"Siyi Li, Zichen Li, Yanfeng Tuo, Guangqing Mu, Shujuan Jiang","doi":"10.1021/acs.jafc.5c07986","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07986","url":null,"abstract":"Two immunomodulatory signature peptides (PFPEVFG and SPAQILQW) were obtained from casein hydrolysates produced by Kluyveromyces marxianus JY-1 in our previous studies with the aid of virtual screening. However, their mechanisms for improving the composition of the gut microbiota in immunosuppressed mice remain unknown. The aim of this study was to reveal the mechanisms of immunomodulation and improvement of gut microbiota composition mediated by the casein peptides PFPEVFG and SPAQILQW through a cyclophosphamide (Cy)-induced immunosuppression and intestinal mucosal injury mouse model. The casein peptides PFPEVFG and SPAQILQW reversed Cy-induced intestinal structural damage and could correct for Cy-induced intestinal flora disorders. Importantly, fecal microbiota transplantation (FMT) confirmed that the casein peptides PFPEVFG and SPAQILQW attenuate immunosuppression by targeting participation in the dynamic balance of the gut microbiota. These results indicated that the casein peptides PFPEVFG and SPAQILQW have the potential to be used as natural medicines for the treatment of immunosuppression.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rk2 Provides Multidimensional Relief for Ulcerative Colitis by Improving Intestinal Barrier Function, Reshaping Gut Microbiota, and Regulating Th17/Treg Balance 人参皂苷Rk2通过改善肠道屏障功能，重塑肠道微生物群，调节Th17/Treg平衡，提供溃疡性结肠炎的多维缓解

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c04210

Luying Tan, Zhuoqiao Li, Junzhe Wu, Hanlin Liu, Ting Yang, Jia Wang, Linxuan Liu, Xiaoyu Geng, Jinping Liu

{"title":"Ginsenoside Rk2 Provides Multidimensional Relief for Ulcerative Colitis by Improving Intestinal Barrier Function, Reshaping Gut Microbiota, and Regulating Th17/Treg Balance","authors":"Luying Tan, Zhuoqiao Li, Junzhe Wu, Hanlin Liu, Ting Yang, Jia Wang, Linxuan Liu, Xiaoyu Geng, Jinping Liu","doi":"10.1021/acs.jafc.5c04210","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04210","url":null,"abstract":"Ulcerative colitis (UC) is a multifactorial inflammatory bowel disease associated with intestinal barrier integrity, gut microbiota, and immune homeostasis. According to previous studies, ginsenoside Rk2 (Rk2) demonstrated anti-inflammatory activity in cocultures of Caco-2 and THP-1 cells. This study was the first to demonstrate that Rk2 positively impacted UC by regulating the “barrier-microbiota-immune” axis. Rk2 not only improved inflammatory factor levels and colitis symptoms but also enhanced intestinal epithelial barrier function via increased transepithelial electrical resistance (TEER) values and elevated expressions of tight junction proteins, remodeled gut microbiota via enriching its diversity and composition and increasing short-chain fatty acids (SCFAs) levels, and maintained intestinal homeostasis via restoring Th17/Treg balance. Moreover, the influence of Rk2 on UC might be mediated via the TNFSF14/LTβR/NIK pathway. In summary, Rk2 exerted a beneficial effect on UC by providing multidimensional regulation and was a potential natural therapeutic agent for UC.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"82 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Lactococcus lactis for the Enhanced Production of Lactate by Manipulating Pyruvate Dehydrogenase Complex E2 and Lactate Dehydrogenase. 乳酸乳球菌通过调控丙酮酸脱氢酶复合物E2和乳酸脱氢酶促进乳酸生成的代谢工程。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c09688

Xiaocui Zhang,Manuel Montalbán-López,Xingjiang Li,Xuefeng Wu,Dongdong Mu

引用次数: 0

Current Progress of Molecularly Imprinted Polymers for Emerging Pollutants Analysis in Food. 分子印迹聚合物在食品中新兴污染物分析中的研究进展。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c04772

Quan-Bin Fu,Xin Sun,Lu Liu,Ru-Song Zhao,Xia Wang

引用次数: 0