Journal of Agricultural and Food Chemistry最新文献_第8页

Intestinal Organoid-Based Toxicity Study on Co-exposure to Food Contaminants Deoxynivalenol and Tenuazonic Acid 食品污染物脱氧雪腐菌烯醇和Tenuazonic酸共同暴露的肠道类器官毒性研究

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-29 DOI: 10.1021/acs.jafc.6c07522

Huikang Lin, Bingxuan Jia, Binhe Chang, Yingzhu Tan, Ye Tian, Na Liu, Aibo Wu

{"title":"Intestinal Organoid-Based Toxicity Study on Co-exposure to Food Contaminants Deoxynivalenol and Tenuazonic Acid","authors":"Huikang Lin, Bingxuan Jia, Binhe Chang, Yingzhu Tan, Ye Tian, Na Liu, Aibo Wu","doi":"10.1021/acs.jafc.6c07522","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c07522","url":null,"abstract":"Environmental mycotoxins threatenhuman and animal health. Deoxynivalenol(DON) and emerging tenuazonic acid (TeA) severely contaminate foodcrops. The intestine is DON’s main toxic target and TeA’sprimary exposure site, but TeA’s intestinal risk and theircombined toxicity remain understudied. In this study, using an intestinalorganoid model, we found that DON and TeA significantly suppressedcell viability (toxicity ratio ∼ 1:20) with synergistic effectsunder high toxicity, disrupting organoid development and intestinalbarrier function (cell cycle arrest, reduced tight junction proteins,increased goblet cells), linked to their ribotoxicity and inducinglipid metabolism disorders (downregulated fatty acid β-oxidationgenes) via the PPAR pathway. Single-cell transcriptome revealed thatthey impaired intestinal cell proliferation/differentiation, inducingribotoxic stress in stem/TA cells and reducing canonical enterocytes(ECs), and ECs were core targets of their lipid toxicity. In conclusion,DON and TeA share intestinal toxicological characteristics, and theircombined exposure potentiates toxicity, requiring targeted measuresto mitigate co-exposure risks.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31840–31857"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149173837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host Volatiles Drive Host Preference and Consequently Shape the Fitness of a Generalist Parasitoid Wasp, Meteorus pulchricornis (Hymenoptera: Braconidae) 寄主挥发物驱动寄主偏好并影响寄主适宜度（膜翅目：小蜂科）

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-14 DOI: 10.1021/acs.jafc.6c06596

Zhen-Xiao Li, Ruo-Lan Hu, Rui-Zhong Yuan, Peng-Zhan Wang, Yu-Si Chen, Zhi-Zhi Wang, Xue-Xin Chen

{"title":"Host Volatiles Drive Host Preference and Consequently Shape the Fitness of a Generalist Parasitoid Wasp, Meteorus pulchricornis (Hymenoptera: Braconidae)","authors":"Zhen-Xiao Li, Ruo-Lan Hu, Rui-Zhong Yuan, Peng-Zhan Wang, Yu-Si Chen, Zhi-Zhi Wang, Xue-Xin Chen","doi":"10.1021/acs.jafc.6c06596","DOIUrl":"10.1021/acs.jafc.6c06596","url":null,"abstract":"Host preference is critical to theefficacy of parasitoidsin biologicalcontrol, while the underlying mechanisms remain unclear. Here, wefound that the host preference of the generalist parasitoid Meteorus pulchricornis revealed an innate preferencefor Spodoptera frugiperda, followedby Plodia interpunctella. GC–MSanalysis showed distinct volatile organic compound profiles betweenthe two hosts, with 2-hexanol as a shared component. Electroantennogram(EAG) recordings showed that wasp antennae responded more stronglyto S. frugiperda than to P. interpunctella odors, suggesting olfaction mediateshost preference. Knockdown of the odorant receptor co-receptor, MpulORco, impaired both EAG responses and host preferencebehavior. Furthermore, wasps reared on S. frugiperda had larger body size and higher fecundity, providing a plausiblefitness-based explanation for the observed preference. Collectively,our study reveals olfaction is involved in host preference behaviorin a generalist parasitoid and establishes a functional connectionbetween olfactory cues and individual fitness.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31394–31404"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148973599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistically Optimized Continuous Stirred-Tank Reactor for Efficient and Scalable Enzymatic Synthesis of Puerarin Palmitate 统计优化的连续搅拌槽反应器用于高效和可扩展的酶合成葛根素棕榈酸酯

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-30 DOI: 10.1021/acs.jafc.6c06098

Eunjeong Yang, Bo Kyeong Kim, Eun Woo Lee, Hyerim Son, Jeongmok Yang, Giwon Lee, Taek Lee, Jong-Min Oh, Si Jae Park, Hah Young Yoo, Chulhwan Park

{"title":"Statistically Optimized Continuous Stirred-Tank Reactor for Efficient and Scalable Enzymatic Synthesis of Puerarin Palmitate","authors":"Eunjeong Yang, Bo Kyeong Kim, Eun Woo Lee, Hyerim Son, Jeongmok Yang, Giwon Lee, Taek Lee, Jong-Min Oh, Si Jae Park, Hah Young Yoo, Chulhwan Park","doi":"10.1021/acs.jafc.6c06098","DOIUrl":"10.1021/acs.jafc.6c06098","url":null,"abstract":"Flavonoid esters are promising bioactive compounds forpharmaceuticaland functional food applications because of their enhanced lipophilicity,stability, and biological activities. However, conventional batchprocesses are limited in their productivity and sustainability. Inthis study, a stable continuous enzymatic acylation system was developedfor the efficient synthesis of flavonoid esters with a particularfocus on puerarin palmitate. Key process variables and their interactionswere systematically evaluated using statistical approaches, and underoptimal conditions, the cumulative conversion and production reached89.66% and 166.80 mg, respectively. The process was implemented ina continuous stirred-tank reactor (CSTR), maintaining an average conversionof 88.54% over 9 h of operation. The total production and productivityof puerarin palmitate were 1921.21 mg and 51.23 g/L/day, respectively,demonstrating the feasibility of continuous production. Ultimately,this study provides a scalable framework for sustainable flavonoidester synthesis in pharmaceutical and food industries.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31748–31756"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gigantol Ameliorates High-Fat and High-Fructose Diet-Induced Chronic Kidney Disease by Promoting TFEB-Mediated Mitophagy to Suppress Ferroptosis 巨巨醇通过促进tfeb介导的线粒体自噬抑制铁下垂来改善高脂肪和高果糖饮食诱导的慢性肾病

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-29 DOI: 10.1021/acs.jafc.6c05807

Yingying Song, Yurou Huang, Yaxin Huang, Yingxi Luo, Han Zhao, Renwu Qin, Simeng Xiao, Cong Zhang

{"title":"Gigantol Ameliorates High-Fat and High-Fructose Diet-Induced Chronic Kidney Disease by Promoting TFEB-Mediated Mitophagy to Suppress Ferroptosis","authors":"Yingying Song, Yurou Huang, Yaxin Huang, Yingxi Luo, Han Zhao, Renwu Qin, Simeng Xiao, Cong Zhang","doi":"10.1021/acs.jafc.6c05807","DOIUrl":"10.1021/acs.jafc.6c05807","url":null,"abstract":"Chronic kidney disease (CKD) is a serious public healthchallengethat closely correlates with unhealthy diets. Gigantol, a naturallyactive compound derived from the edible plant Dendrobium, has shown potential in renal protection, but its efficacy in treatingmetabolic dysfunction-related CKD driven by a high-fat and high-fructosediet (HFFD) remains unclear. Here, we found that gigantol restoredabnormal renal function and pathological manifestations in HFFD-fedmice. Mechanistically, gigantol suppresses the phosphorylation ofTFEB at Ser211 and promotes TFEB nuclear translocation to enhancePINK1/Parkin-mediated mitophagy and suppress ferroptosis. Similarfindings were observed in palmitic-acid-induced cell models. Notably,silencing TFEB or blocking mitophagy reversed the effect of gigantolon suppressing ferroptosis and improving renal injury. Overall, thisstudy demonstrates that gigantol alleviates metabolic-dysfunction-relatedCKD in mice by promoting TFEB-mediated mitophagy to suppress ferroptosis,suggesting that dietary supplementation with Dendrobium or gigantol represents an effective strategy in mitigating CKD,especially subtypes driven by metabolic dysfunction.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31630–31646"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium asiaticum FaSdhC2 Point Mutation A83V Confers Resistance to Pydiflumetofen and Development of a Rapid LAMP Detection Method 亚洲镰刀菌FaSdhC2点突变A83V对吡氟醚的抗性及LAMP快速检测方法的建立

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-02 DOI: 10.1021/acs.jafc.6c09029

Yudi Ma, Qiaowan Chen, Guiping Zhang, Xin Chen, Chaowen Zhang, Shuodan Hu, Zhonghua Ma

{"title":"Fusarium asiaticum FaSdhC2 Point Mutation A83V Confers Resistance to Pydiflumetofen and Development of a Rapid LAMP Detection Method","authors":"Yudi Ma, Qiaowan Chen, Guiping Zhang, Xin Chen, Chaowen Zhang, Shuodan Hu, Zhonghua Ma","doi":"10.1021/acs.jafc.6c09029","DOIUrl":"10.1021/acs.jafc.6c09029","url":null,"abstract":"Fusarium head blight (FHB) causedby Fusarium graminearum species complex (FGSC) isa devastating disease worldwide. The newsuccinate dehydrogenase inhibitor pydiflumetofen is highly effectiveagainst FHB, but its growing use raises resistance concerns. In thisstudy, we identified four resistant Fusarium asiaticum isolates from 4088 field isolates collected across eight provincesin China in 2025. Gene replacement tests confirmed that the single-pointmutation A83V of FaSdhC2 confers resistance. Notably, the resistantisolates did not exhibit significant fitness costs related to pydiflumetofen-resistancein terms of mycelial growth, sporulation, pathogenicity, and stresstolerance. To facilitate field monitoring, we developed a loop-mediatedisothermal amplification (LAMP) assay targeting the FaSdhC2A83V mutation. This method is easy to operate, provides visual resultswithin 55 min of amplification and is 100-fold more sensitive thanconventional PCR. In conclusion, the LAMP assay established in thisstudy provides a practical tool for rapid on-site monitoring of fungicideresistance and can effectively guide the rational use of fungicidesin the field.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31491–31502"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut Microbiota Remodeling Drives Cognitive Improvement: A Microbiota-Dependent Study of Malvidin-3-O-glucoside against Alcohol-Related Cognitive Impairment 肠道菌群重塑驱动认知改善：马尔维丁-3- o -葡萄糖苷对酒精相关认知障碍的微生物依赖研究

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-08-27 DOI: 10.1021/acs.jafc.6c08126

Yuhan Du, Qi Li, Fan Yang, Bo Zhang, Mingming Lei, Dan Zhao, Xuanwei Xu, Yang He, Liankui Wen

{"title":"Gut Microbiota Remodeling Drives Cognitive Improvement: A Microbiota-Dependent Study of Malvidin-3-O-glucoside against Alcohol-Related Cognitive Impairment","authors":"Yuhan Du, Qi Li, Fan Yang, Bo Zhang, Mingming Lei, Dan Zhao, Xuanwei Xu, Yang He, Liankui Wen","doi":"10.1021/acs.jafc.6c08126","DOIUrl":"10.1021/acs.jafc.6c08126","url":null,"abstract":"Gutmicrobiota dysbiosis triggers alcohol-related cognitive impairment(ARCI). Malvidin-3-O-glucoside (Mv3G), a highly bioactivemonomeric anthocyanin abundant in pigmented fruits and cereals, canrelieve neurodegenerative diseases by regulating gut microbiota (GM).Nevertheless, the gut microbiota-mediated protective effect of Mv3Gagainst ARCI remains unknown. In this study, an alcohol-induced ARCImouse model combined with fecal microbiota transplantation (FMT) wasbuilt to explore Mv3G’s protective effects. Results show thatMv3G acts in an intact gut flora-dependent manner. It repairs intestinalinjury and restores intestinal barrier by upregulating tight junctionproteins ZO-1 and occludin, enriches beneficial bacteria such as Lachnospiraceae_NK4A136_group and Akkermansia, adjusts the Bacteroidetes/Firmicutes ratio, and decreases pathogenic Pseudomonadales to remodel gut microbiota. Via the gut–brainaxis, Mv3G suppresses central inflammation, maintains hippocampalsynapses and improves cognitive performance. In vitro experimentsfurther confirm that Mv3G alleviates ARCI relying on gut microbiota.This research provides a novel intervention strategy for alcohol-inducedcognitive injury.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31679–31703"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148822326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Di-2-ethylhexyl Phthalate Induces Neurotoxicity of Transforming Microglia into M1 Subtype through Targeting α-Syn Dimerization 邻苯二甲酸二-2-乙基己基酯通过靶向α-聚合诱导小胶质细胞转化为M1亚型的神经毒性

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-22 DOI: 10.1021/acs.jafc.6c08806

Ying-Xin Zhao, Ke-Tian Zhu, Yu-Xuan Tong, Qi-Qian Wang, Jin-Long Li

{"title":"Di-2-ethylhexyl Phthalate Induces Neurotoxicity of Transforming Microglia into M1 Subtype through Targeting α-Syn Dimerization","authors":"Ying-Xin Zhao, Ke-Tian Zhu, Yu-Xuan Tong, Qi-Qian Wang, Jin-Long Li","doi":"10.1021/acs.jafc.6c08806","DOIUrl":"10.1021/acs.jafc.6c08806","url":null,"abstract":"Di-2-ethylhexyl phthalate (DEHP) accumulates in the foodchain,threatening human and animal health. However, how DEHP induces neurotoxicityby interfering with microglia has not been fully revealed. Our researchaims to explore the effects of DEHP on microglia and identify relevanttargets that cause neurotoxicity. The results indicated that DEHPdamaged neurons, leading to swelling, demyelination, and abnormalautophagy. Inhibition of autophagy resulted in α-Syn misfolding,characterized by an increase in dimers with increasing additive concentrations(P = 0.0096). Dimer formation was promoted by theaddition of additives, which increased the electrostatic force, alongwith interactions between Glu106, Gly51, and Val66. Transmitted fromneurons to microglia (Pearson r2 = 0.889),pathological aggregates under DEHP exposure induced microglial M1subtype switching. This study elucidated a novel mechanism by whichDEHP damages the nerves of birds and provided a therapeutic targetfor the neurotoxic disease risk caused by the transmission of plasticadditives through the food chain.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31858–31869"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149022119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin Alleviates TMT-Induced PANoptosis in Chicken Skeletal Muscle through Modulation of the ROS/mtDNA Axis 槲皮素通过调节ROS/mtDNA轴减轻estmt诱导的鸡骨骼肌PANoptosis

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-30 DOI: 10.1021/acs.jafc.6c06054

Chengguo Liu, Ping Zhang, Yue Zhai, Fangfang Wu, Jiahui Duan, Tong Xu, Lixia Zhao

{"title":"Quercetin Alleviates TMT-Induced PANoptosis in Chicken Skeletal Muscle through Modulation of the ROS/mtDNA Axis","authors":"Chengguo Liu, Ping Zhang, Yue Zhai, Fangfang Wu, Jiahui Duan, Tong Xu, Lixia Zhao","doi":"10.1021/acs.jafc.6c06054","DOIUrl":"10.1021/acs.jafc.6c06054","url":null,"abstract":"Trimethyltin(TMT) is an environmental organotin contaminant withrecognized systemic toxicity, but its skeletal muscle effects andthe protective mechanism of quercetin (Que) remain unclear. This studyintegrated network toxicology, molecular docking, molecular dynamicssimulations, and in vivo/in vitro validation to investigate Que-mediatedprotection against TMT-induced skeletal-muscle injury. Network analysisindicated the involvement of oxidative stress and cytosolic DNA-sensingpathways, while docking and dynamic simulations suggested stable interactionsbetween Que and mitochondrial regulators and PANoptosis-associatedproteins. In vivo and in vitro validation showed that TMT inducedskeletal muscle injury, reactive oxygen species (ROS) accumulation,mitochondrial dynamic imbalance, respiratory chain dysfunction, mitochondrialDNA (mtDNA) leakage, and PANoptosis activation. Que markedly suppressedROS generation, restored mitochondrial homeostasis, reduced cytosolicmtDNA leakage, and inhibited PANoptosis-related signaling. Collectively,Que alleviates TMT-induced skeletal muscle toxicity by regulatingthe ROS/mtDNA axis, supporting its potential as a dietary interventionor feed additive against organotin-associated muscle damage.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31824–31839"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vicia faba TEOSINTE BRANCHED1/CYCLOIDEA/PCF Transcription Factors VfTCP19, VfTCP21 and VfTCP23 Contribute to Biotic and Abiotic Stress-Associated Responses 蚕豆TEOSINTEBRANCHED1/CYCLOIDEA/PCF转录因子VfTCP19、VfTCP21和vftcp23参与生物和非生物应激相关反应

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-09-30 DOI: 10.1021/acs.jafc.6c06084

Junliang Yin, Hongguang Zhu, Shi Chen, Xiaohui Yin, Yi Yuan, Yiting Li, Yongxing Zhu

{"title":"Vicia faba TEOSINTE BRANCHED1/CYCLOIDEA/PCF Transcription Factors VfTCP19, VfTCP21 and VfTCP23 Contribute to Biotic and Abiotic Stress-Associated Responses","authors":"Junliang Yin, Hongguang Zhu, Shi Chen, Xiaohui Yin, Yi Yuan, Yiting Li, Yongxing Zhu","doi":"10.1021/acs.jafc.6c06084","DOIUrl":"10.1021/acs.jafc.6c06084","url":null,"abstract":"Faba bean provides food and feed,also improves field soil. However,its sustainable production is severely constrained by adverse environmentalconditions. Identification desired genes could provide resources forresistance breeding, thus improve field performance. Here, 24 VfTCPtranscription factors were identified and characterized as nucleus-localized,unstable, hydrophilic proteins. VfTCPs were under strong purifyingselection, and their promoters contained cis-elementsrelated to growth, development, and stress responses. Transcriptomeprofiling and RT-qPCR confirmed their stress-responsive expressionpatterns. Experimental analyses confirmed the nuclear localizationand GAL4-based transcriptional activation activity of VfTCP19/21/23. Transient overexpression of VfTCP19/21/23 contributedto pathogen resistance by maintaining ROS homeostasis and attenuatingBAX-, NIP-, and INF1-induced. Meanwhile, they enhanced drought andsalt tolerance by regulating stomatal aperture, strengthening waterretention, improving water status, and scavenging ROS, thereby alleviatingleaf wilting. These findings revealed VfTCP19/21/23 as potential candidate genes for future stress-resistance breeding.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31374–31393"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms Underlying Differential Flusulfinam Sensitivity between Oryza sativa and Echinochloa crus-galli 水稻和紫球藻对氟磺胺敏感性差异的机制

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-14 Epub Date: 2026-10-05 DOI: 10.1021/acs.jafc.6c08522

Hengzhi Wang, Ping Yin, Yutong Pan, Longhao Zou, Yunxia Duan, Weitang Liu

{"title":"Mechanisms Underlying Differential Flusulfinam Sensitivity between Oryza sativa and Echinochloa crus-galli","authors":"Hengzhi Wang, Ping Yin, Yutong Pan, Longhao Zou, Yunxia Duan, Weitang Liu","doi":"10.1021/acs.jafc.6c08522","DOIUrl":"10.1021/acs.jafc.6c08522","url":null,"abstract":"Flusulfinam is a novel postemergence HPPD-inhibitingherbicidewith excellent safety on direct-seeded indica rice, but the mechanismunderlying its selectivity between rice and Echinochloa crus-galli remains unclear. Rice exhibited 41–141-foldhigher GR50 values than E. crus-galli, with cultivar-dependent tolerance. Residue analysis showed higherflusulfinam absorption in E. crus-galli, whereas metabolism was significantly enhanced in rice and inhibitedby P450 and GST inhibitors. Transcriptomic and qRT-PCR analyses identified OsGSTU16 as highly expressed in rice compared with E. crus-galli. Functional validation using Escherichia coli expression, rice callus overexpression,knockout experiments, and molecular docking demonstrated that OsGSTU16 contributes to flusulfinam tolerance. These findingssuggest that GST-mediated metabolic detoxification is an importantcontributor to flusulfinam selectivity and provide insights into potentialgenetic resources for improving herbicide tolerance in crops.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 40","pages":"31704–31714"},"PeriodicalIF":6.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149091117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0