Journal of Agricultural and Food Chemistry最新文献_第8页

Reference Gene-Assisted LAMP–LFD for Sensitive and Specific Detection of Soy DNA as a Marker for Allergen Presence in Complex Food Products 参考基因辅助LAMP-LFD灵敏特异检测大豆DNA作为复杂食品中过敏原存在的标记

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-24 DOI: 10.1021/acs.jafc.5c01463

Qiaofeng Li, Marleen M. Voorhuijzen-Harink, Dianpeng Han, Bas J. Fronen, Richard van Hoof, Ming Chen, Zhouping Wang, Toine F. H. Bovee, Zhixian Gao, Gert IJ. Salentijn

{"title":"Reference Gene-Assisted LAMP–LFD for Sensitive and Specific Detection of Soy DNA as a Marker for Allergen Presence in Complex Food Products","authors":"Qiaofeng Li, Marleen M. Voorhuijzen-Harink, Dianpeng Han, Bas J. Fronen, Richard van Hoof, Ming Chen, Zhouping Wang, Toine F. H. Bovee, Zhixian Gao, Gert IJ. Salentijn","doi":"10.1021/acs.jafc.5c01463","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01463","url":null,"abstract":"Sensitive, selective screening for allergenic ingredients with internal control is crucial to identify food adulteration and remove allergens from the food chain. Here, loop-mediated isothermal amplification combined with a lateral flow device (LAMP–LFD) was developed for the fast and easy detection of soy DNA. The integrated quality controls included a regular control line to ensure proper implementation (extraction and amplification control) and a second LAMP–LFD assay for the cytochrome oxidase gene, which is a housekeeping gene in plants. The developed LAMP assay showed a limit of detection of only 5 pg DNA input per reaction, for both pure soy and spiked food samples. The test was subsequently implemented for the examination of 32 real food products with different compositions and declared soy contents, and benchmarked against qPCR. Then, this system was combined with a digital cube reader, allowing direct interpretation of the test results, facilitating the point-of-need applicability.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"8 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Profiling of a Mediterranean-Inspired (Poly)phenol-Rich Mixture in the Brain: Perfusion Effect and In Vitro Blood–Brain Barrier Transport Validation 地中海启发的（多）富酚混合物在脑中的代谢谱：灌注效应和体外血脑屏障运输验证

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-24 DOI: 10.1021/acs.jafc.5c02288

María Ángeles Ávila-Gálvez, Beatriz Garay-Mayol, Alicia Marín, María Alexandra Brito, Juan Antonio Giménez-Bastida, Juan Carlos Espín, Antonio González-Sarrías

{"title":"Metabolic Profiling of a Mediterranean-Inspired (Poly)phenol-Rich Mixture in the Brain: Perfusion Effect and In Vitro Blood–Brain Barrier Transport Validation","authors":"María Ángeles Ávila-Gálvez, Beatriz Garay-Mayol, Alicia Marín, María Alexandra Brito, Juan Antonio Giménez-Bastida, Juan Carlos Espín, Antonio González-Sarrías","doi":"10.1021/acs.jafc.5c02288","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02288","url":null,"abstract":"A Mediterranean diet rich in (poly)phenols has been linked to neuroprotection, but its effects likely depend on the ability of phenolic metabolites to cross the blood–brain barrier (BBB). This study evaluated the kinetics plasma and brain distribution of phenolic metabolites in Sprague–Dawley rats following oral administration of a polyphenol-rich extract mixture from Mediterranean foods (pomegranate, lemon, orange, grape, and olive). UPLC-ESI-QTOF analyses revealed 39 phenolic-derived metabolites in plasma, of which 20 were in nonperfused (NPB) and 19 in perfused brains (PB), including hydroxytyrosol and tyrosol sulfates, ellagic acid, dihydrocaffeic acid, and derived metabolites. Kinetic data showed substantially higher plasma metabolite concentrations than the brain, with slightly higher levels in NPB. The BBB transport efficiency of phenolic metabolites was validated in vitro using human brain microvascular endothelial cells (HBMECs), showing improved transport when tested as mixtures. These findings confirm that circulating phenolic metabolites from Mediterranean foods can reach brain tissues, contributing to preventing neurodegenerative diseases.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"56 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Heavy Metal Copper Stress in Apple Rootstocks Using Surface-Enhanced Raman Spectroscopy 利用表面增强拉曼光谱检测苹果根茎中的重金属铜应力

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.5c00126

Junmeng Li, Hongpu Guan, Yibo Zhou, Shouhao Pei, Keqiang Yu, Yanru Zhao

{"title":"Detection of Heavy Metal Copper Stress in Apple Rootstocks Using Surface-Enhanced Raman Spectroscopy","authors":"Junmeng Li, Hongpu Guan, Yibo Zhou, Shouhao Pei, Keqiang Yu, Yanru Zhao","doi":"10.1021/acs.jafc.5c00126","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00126","url":null,"abstract":"Excessive use of copper (Cu) chemicals has led to soil contamination. This study utilized surface-enhanced Raman spectroscopy (SERS) to investigate the effects of 10 commonly encountered concentrations of Cu stress in orchards on apple rootstocks. Spectral preprocessing methods were employed to eliminate baseline drift and fluorescence background interference from the Raman spectra, while data augmentation techniques were incorporated to develop a one-dimensional stacked autoencoder convolutional neural network (1D-SAE-CNN) for classifying Cu stress levels, resulting in evaluation indices greater than 0.9. Scanning electron microscopy with energy dispersive spectroscopy (SEM–EDS) quantified Cu distribution in root, stem, and leaf tissues, while micro-Raman imaging visualized lignin, cellulose, and pigments under Cu stress. The results indicate that SERS combined with a deep learning model enables rapid and accurate differentiation of Cu stress levels in apple rootstocks in orchards, while SEM–EDS and micro-Raman imaging techniques reveal the migration effect of Cu2+ within apple rootstock tissues and the ″low concentration promotion, high concentration inhibition″ effect of Cu on apple rootstock growth. Therefore, this approach showcases rapid and accurate detection of heavy metal Cu stress in apple rootstock tissues and has great potential for analyzing various types of heavy metal pollution in agricultural orchard ecosystems.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"32 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical Sensing toward Noninvasive Evaluation of High-Starch Food Digestion via Point-of-Use Monitoring Glucose Level in Saliva 通过监测唾液中的葡萄糖水平，电化学传感对高淀粉食物消化的无创评估

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.5c02597

Haili Chen, Xiaowei Huang, Yongqiang Shi, Yahui Li, Weilong Tan, Xinai Zhang, Yucheng Zou, Tianxing Wang, Jiyong Shi, Xiaobo Zou

{"title":"Electrochemical Sensing toward Noninvasive Evaluation of High-Starch Food Digestion via Point-of-Use Monitoring Glucose Level in Saliva","authors":"Haili Chen, Xiaowei Huang, Yongqiang Shi, Yahui Li, Weilong Tan, Xinai Zhang, Yucheng Zou, Tianxing Wang, Jiyong Shi, Xiaobo Zou","doi":"10.1021/acs.jafc.5c02597","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02597","url":null,"abstract":"To provide individuals with healthier and more reliable dietary recommendations for diabetic patients, a pragmatic electrochemical sensing toward in situ monitoring of glucose in saliva was designed for noninvasive evaluation of high-starch food digestion. The proposed sensor was constructed by exploiting a carbon-based nanostructure for electrical conductivity and Ni-based nanozyme toward direct catalytic oxidation of glucose for signal output. Particularly, the catalytic host–guest interaction between nanozyme and glucose was investigated to be a hydrogen bond via molecular docking, and the C11 and O5 sites in the glucose molecule were attacked during host–guest catalytic reaction through density functional theory (DFT) research. With merits of simplicity, sensitivity, and accuracy, the electrochemical sensor exhibited good performance for monitoring glucose with a detection limit of 10 μM (corresponding to 1.8 μg mL–1). Moreover, it was well qualified to point-of-use trace glucose levels in saliva, offering a promising tool for noninvasive evaluation of high-starch food digestion (e.g., Wheat bread, Steamed bun, and Shao-mai) at different times after meals and eventually yielding benefits to dietary recommendations for diabetic patients.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"51 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Alcohol Dehydrogenase for Efficient Catalytic Synthesis of Ethyl (R)-4-Chloro-3-hydroxybutyrate 工程醇脱氢酶高效催化合成(R)-4-氯-3-羟基丁酸乙酯

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.5c00471

Pei Zhou, Mengxue Wu, Lan Ma, Yi Li, Xiaotong Liu, Zongda Chen, Yifan Zhao, Zisen Li, Luxi Zheng, Yang Sun, Yinbiao Xu, Yupeng Liu, Hua Li

{"title":"Engineering Alcohol Dehydrogenase for Efficient Catalytic Synthesis of Ethyl (R)-4-Chloro-3-hydroxybutyrate","authors":"Pei Zhou, Mengxue Wu, Lan Ma, Yi Li, Xiaotong Liu, Zongda Chen, Yifan Zhao, Zisen Li, Luxi Zheng, Yang Sun, Yinbiao Xu, Yupeng Liu, Hua Li","doi":"10.1021/acs.jafc.5c00471","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00471","url":null,"abstract":"Ethyl (R)-4-chloro-3-hydroxybutyrate [(R)–CHBE] is an intermediate with high value in medicine and pesticide applications. Alcohol dehydrogenase serves as an excellent biocatalyst during the synthesis of (R)–CHBE. However, the lack of effective engineering methods limits its wider application. In this study, the sequence-modeling-docking-principle (SMDP) method was used to screen enzymes with catalytic activity. Three protein modification strategies were established for the active center, substrate channel, and distal hotspot to enhance the catalytic efficiency of alcohol dehydrogenase LCRIII. Substrate batch replenishment was used to alleviate substrate inhibition. Subsequently, optimal mutant M3 (W151F–S167A-F215Y) was successfully obtained with a specific enzyme activity of 23.00 U/mg and kcat/Km of 11.22 (mM–1·min–1), which were 4.55- and 3.98-fold higher than those of the wild type, respectively. (R)–CHBE was prepared using M3 and GDH at 298.21 g/L (>99% e.e.). This study provides a promising approach for the protein engineering modification of alcohol dehydrogenase and industrial-scale production of (R)–CHBE.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"67 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking Loop Geometric Remodeling Redirects Aldo-Keto Reductase from Substrate Promiscuity to 3-Keto-Deoxynivalenol Specificity 解锁环几何重构将醛酮还原酶从底物混杂重定向到3-酮-脱氧雪梨酚特异性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.4c12497

Bin Ma, Jiafeng Niu, Hao Zhu, Huibing Chi, Zhaoxin Lu, Fengxia Lu, Ping Zhu

{"title":"Unlocking Loop Geometric Remodeling Redirects Aldo-Keto Reductase from Substrate Promiscuity to 3-Keto-Deoxynivalenol Specificity","authors":"Bin Ma, Jiafeng Niu, Hao Zhu, Huibing Chi, Zhaoxin Lu, Fengxia Lu, Ping Zhu","doi":"10.1021/acs.jafc.4c12497","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12497","url":null,"abstract":"Aldo-keto reductase is responsible for the formation of nontoxic 3-epi-deoxynivalenol (3-epi-DON) from 3-keto-DON, which is an enzymatic detoxification manner to completely eliminate the toxicity of mycotoxin DON against health and environmental threats. Therefore, unlocking the facilitated substrate specificity of aldo-keto reductase for 3-keto-DON has become a critical challenge for advanced catalytic performance. In this endeavor, a loop-based engineering strategy was developed for aldo-keto reductase AKR13B3 from Devosia A6–243 to catalyze 3-keto-DON. A 31.9-fold switch from a disfavored substrate to the preferred one was produced along with a significant 37.9-fold increase in catalytic efficiency. Kinetic parameter determinations, structural analyses, and molecular simulations were employed to elucidate the molecular mechanisms underlying these enhancements in catalytic activity and substrate specificity. Overall, our work presents a feasible scheme for designing aldo-keto reductases with exceptional substrate specificity and catalytic activity, holding great promise for developing enzymatic detoxification agents.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"31 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design and Engineering of 3-O-Sulfotransferase 1 Based on Enzyme Affinity for Improved Enzymatic Heparin Preparation 基于酶亲和力的3- o -硫基转移酶1的合理设计与工程

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.4c07514

Tiansu Tan, Jing Yu, Jianyu Long, Xiaojing Li, Zheng-Jun Li, Yang Zhang, Mingjia Yu, Tianwei Tan

{"title":"Rational Design and Engineering of 3-O-Sulfotransferase 1 Based on Enzyme Affinity for Improved Enzymatic Heparin Preparation","authors":"Tiansu Tan, Jing Yu, Jianyu Long, Xiaojing Li, Zheng-Jun Li, Yang Zhang, Mingjia Yu, Tianwei Tan","doi":"10.1021/acs.jafc.4c07514","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07514","url":null,"abstract":"Heparin, a naturally occurring glycosaminoglycan, is renowned for its potent anticoagulant properties, which are critical for various medical applications. A significant determinant of its anticoagulant activity is the degree of 3-O-sulfation. Gaining insight into the substrate binding characteristics of 3-O-sulfotransferase-1 (3-OST-1) could enhance our understanding of the sulfotransferase family and facilitate the enzymatic preparation of heparin. This study aimed to identify mutants of 3-OST-1 with improved catalytic activities through a rational design. The enzyme activities of the mutants W72R and H144R were recorded at 26.40 and 17.21 U/L, respectively, representing increases of 1.7 and 1.1 times compared to the wild-type (WT) 3-OST-1. Notably, the enzyme activity of the double mutant W72R/H144R reached 34.41 U/L, which is 2.2 times greater than that of the WT. The heparin modified by the 3-OST-1 mutants exhibited superior anticoagulant properties compared with those modified by the WT, with W72R/H144R demonstrating the highest anticoagulant potency. Furthermore, enzyme kinetic assays and molecular dynamics simulations illustrated that the enhanced catalytic activity of the mutant enzyme resulted from an increased affinity for the substrate.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"109 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioaccessible (Poly)phenols of Winery Byproducts Modulate Pathogenic Mediators of Intestinal Bowel Disease: In Vitro Evidence 酒庄副产品的生物可及（多）酚调节肠道疾病的致病介质：体外证据

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.5c00916

Vicente Agulló, Cristina García-Viguera, Sonia Medina, Raúl Domínguez-Perles

{"title":"Bioaccessible (Poly)phenols of Winery Byproducts Modulate Pathogenic Mediators of Intestinal Bowel Disease: In Vitro Evidence","authors":"Vicente Agulló, Cristina García-Viguera, Sonia Medina, Raúl Domínguez-Perles","doi":"10.1021/acs.jafc.5c00916","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00916","url":null,"abstract":"Intestinal inflammation entails a multifactorial pathophysiology, frequently treated by using anti-inflammatory drugs with severe side effects. At the same time, bioactive compounds present in plant materials and derived residues could contribute to reducing the use of such medications in terms of dosage and treatment length. Thus, the phytochemicals of winery byproducts, mainly represented by (poly)phenols, display significant anti-inflammatory and antioxidant potential. However, the functionality of bioaccessible fractions remains underexplored. This study uncovers the capacity of bioaccessible (poly)phenols of winery byproducts to modulate inflammatory mediators and secondary oxidative stress (OS). After in vitro simulated digestion, bioaccessible (poly)phenols exhibited significant inhibitory capacity of nitric oxide, interleukin (IL)-6, IL-8, and TNF-α production and prevented OS, lowering reactive oxygen species (ROS) resulting from disturbed cell metabolism while preserving the molecular machinery of cells, involving glutathione, catalase, superoxide dismutase, and glutathione peroxidase. The results retrieved suggested the relevance of specific profiles for efficiently preventing inflammation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"5 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutathione S-Transferase Gene GST1 Metabolizing Penoxsulam and Conferring Penoxsulam Resistance in Echinochloa phyllopogon 谷胱甘肽s -转移酶基因GST1代谢培诺舒南并赋予培诺舒南抗性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.5c01661

Haitao Gao, Jianguo Wei, Xinliang Zhang, Jiaxing Yu, Jie Li, Zhike Feng, Yucheng Gu, Liyao Dong

{"title":"Glutathione S-Transferase Gene GST1 Metabolizing Penoxsulam and Conferring Penoxsulam Resistance in Echinochloa phyllopogon","authors":"Haitao Gao, Jianguo Wei, Xinliang Zhang, Jiaxing Yu, Jie Li, Zhike Feng, Yucheng Gu, Liyao Dong","doi":"10.1021/acs.jafc.5c01661","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01661","url":null,"abstract":"Echinochloa phyllopogon, a malignant weed found in paddy fields, has developed resistance to many acetolactate synthase-inhibiting herbicides. This resistance threatens the effectiveness and sustainability of herbicides. Glutathione S-transferases (GSTs) are important detoxification enzymes responsible for herbicide resistance in plants. Here, we identified and characterized two GST genes (EpGST1 and EpGSTU6) that showed higher expression levels in resistant than in susceptible E. phyllopogon populations. Nucleotide sequence alignment showed that EpGST1 was identical in both the sensitive and resistant populations without any mutations. The HPLC-MS/MS results showed that the in vitro recombinant GST1 protein had a stronger ability to metabolize the herbicide penoxsulam than the empty vector or EpGSTU6. Overexpression of EpGST1 in Arabidopsis and rice significantly enhanced penoxsulam resistance. Peroxidase, superoxide dismutase, and catalase activities in the resistant population were significantly enhanced after penoxsulam treatment, and the contents of MDA were significantly increased. Similarly, in rice overexpressing EPGST1, the activities of the antioxidant enzymes peroxidase, superoxide dismutase, and catalase were significantly enhanced, and the contents of H2O2 were significantly decreased. The results of the subcellular localization study indicated that EpGST1 is a nuclear gene. These results suggest that EpGST1 plays a significant role in the evolution of penoxsulam resistance by enhancing herbicide metabolism in E. phyllopogon.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"6 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cAMP-Mediated Biofilm eDNA Transfer Facilitates the Resilience of Soil Microbiome to Agrochemical Stress camp介导的生物膜eDNA转移促进土壤微生物组对农化胁迫的恢复力

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-23 DOI: 10.1021/acs.jafc.5c00961

Zhengyi Yang, Huixin Chen, Guo-Hua Zhong, Jie Liu

{"title":"cAMP-Mediated Biofilm eDNA Transfer Facilitates the Resilience of Soil Microbiome to Agrochemical Stress","authors":"Zhengyi Yang, Huixin Chen, Guo-Hua Zhong, Jie Liu","doi":"10.1021/acs.jafc.5c00961","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00961","url":null,"abstract":"Soil microorganisms utilize extracellular DNA (eDNA)-based biofilms as a defense against xenobiotics. However, the specific effects and transfer pathways of eDNA under persistent agrochemical exposure remain unclear. This study examined the transfer dynamics of carbofuran-hydrolase gene pchA from Pseudomonas stutzeri PS21. During biofilm formation, pchA was released from eDNA, leading to an enrichment of beneficial microorganisms such as Acidobacteria and Elusimicrobia, which enhanced organic compound metabolism and improved soil microbiome resilience. An increase in the pchA-associated mobile genetic elements and the colocalization of pchA with other bacterial species indicated the potential horizontal gene transfer (HGT) under carbofuran exposure. Additionally, carbofuran triggered a cAMP-dependent apoptotic pathway, leading to a 59.6% increase in pchA copy number, which suggested that cAMP played a role in initiating HGT. In conclusion, the cAMP-mediated interspecific transfer of pchA could enhance microbial coadaptation to carbofuran contamination, thereby strengthening the collective defense of soil microbiome against agrochemical stress.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0