乳酸乳球菌通过调控丙酮酸脱氢酶复合物E2和乳酸脱氢酶促进乳酸生成的代谢工程。

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI:10.1021/acs.jafc.5c09688

Xiaocui Zhang,Manuel Montalbán-López,Xingjiang Li,Xuefeng Wu,Dongdong Mu

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引用次数: 0

摘要

乳酸乳球菌中的丙酮酸脱氢酶复合物（PDHc）由pdh E1α， E1β和E2组成，将糖酵解与三羧酸循环（TCA循环）联系起来。本研究探讨了敲除pdh E2对代谢的影响。与野生型相比，敲除菌株生物量为76%，PDHc活性降低48%，ATP降低13.4%，但NAD+/NADH比值提高1.5倍。敲除菌株乳酸产量增加5.6%，过表达菌株乳酸产量增加45.3%。其次，乳酸脱氢酶活性的诱导，在诱导48 h后，添加5 ng/mL nisin时酶量最高（比野生型乳酸乳杆菌提高28.7%）。此外，与丙酮酸代谢途径相关的基因转录水平也发生了变化：pdh E2下降67.62%，而pdh E1β上调15倍。我们的研究为提高乳酸生成和了解pdh E2功能提供了见解。

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Metabolic Engineering of Lactococcus lactis for the Enhanced Production of Lactate by Manipulating Pyruvate Dehydrogenase Complex E2 and Lactate Dehydrogenase.

The pyruvate dehydrogenase complex (PDHc) in Lactococcus lactis, composed of pdh E1α, E1β, and E2, links glycolysis to the tricarboxylic acid cycle (TCA cycle). This study investigated the effect of the pdh E2 knockout on metabolism. The knockout strain showed 76% biomass, 48% lower PDHc activity, and 13.4% reduced ATP versus wild type but a 1.5-fold higher NAD+/NADH ratio. Lactate production increased by 5.6% in the knockout and 45.3% in overexpressing strains. Next to this, the lactate dehydrogenase activity was induced, achieving the highest enzyme amount with the addition of 5 ng/mL nisin after 48 h of induction (28.7% higher than that of wild-type L. lactis). In addition, the transcript levels of genes related to the pyruvate metabolic pathway also changed: pdh E2 decreased by 67.62%, while pdh E1β was upregulated 15-fold. Our study provides insights for enhancing lactate production and understanding pdh E2 function.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.