Journal of Agricultural and Food Chemistry最新文献_第7页

Aflatoxin B1 Induces Pyroptosis and Apoptosis in Renal Cells by Mediating Mitophagy Dysfunction and Mitochondrial Pore Formation 黄曲霉毒素B1介导线粒体自噬功能障碍和线粒体孔形成诱导肾细胞热亡和凋亡

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c07992

Xinyu Yao, Min Gao, Jing Lu, Xuming Deng, Shuang Guan

{"title":"Aflatoxin B1 Induces Pyroptosis and Apoptosis in Renal Cells by Mediating Mitophagy Dysfunction and Mitochondrial Pore Formation","authors":"Xinyu Yao, Min Gao, Jing Lu, Xuming Deng, Shuang Guan","doi":"10.1021/acs.jafc.5c07992","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07992","url":null,"abstract":"Aflatoxin B1 (AFB1), a potent mycotoxin, induces nephrotoxicity through previously unrecognized crosstalk between pyroptosis and apoptosis. Using in vivo and in vitro renal injury models, we demonstrate that AFB1 impairs mitophagy, leading to an excessive level of reactive oxygen species (ROS) accumulation. This ROS surge triggers lysosomal membrane permeabilization (LMP) and cathepsin B (CTSB)-dependent activation of the NOD-like receptor protein 3 (NLRP3) inflammasome, initiating caspase-1–mediated pyroptosis via gasdermin D N-terminal (GSDMD-N) pore formation. Importantly, AFB1 also induces cardiolipin translocation to the mitochondrial outer membrane, where pyroptosis-derived GSDMD-N is recruited to form mitochondrial pores. This results in cytochrome c (Cyt-c) release and activation of a caspase-dependent noncanonical apoptotic cascade distinct from the classical apoptotic pathway. These findings establish GSDMD-N-mediated mitochondrial damage as a molecular bridge linking pyroptosis to apoptosis in AFB1 nephrotoxicity and highlight GSDMD-N inhibition as a promising therapeutic strategy. Given AFB1’s persistence and bioaccumulation in the food chain, these mechanistic insights provide a molecular basis for developing targeted interventions to mitigate its health risks in agricultural production and food safety.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"40 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postbiotics as an Innovative Intervention Strategy for Ulcerative Colitis: Insights into Bioactive Components and Mechanisms of Action. 后生物制剂作为溃疡性结肠炎的创新干预策略：对生物活性成分和作用机制的见解。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c08885

Tianxiang Xue,Yanli Zhu,Mengwei Chang,Yufeng He,Guangsu Zhu

引用次数: 0

Functional Characterization and Protein Engineering of Salvia miltiorrhiza Rosmarinic Acid Synthase. 丹参迷迭香酸合成酶的功能表征及蛋白工程研究。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c08090

Yue Feng,Zhoulu Wang,Junbo Li,Zhenli Ren,Yanting Wu,Hao Zhan,Xuefei Chen,Yao Wang,Min Shi,Xu Jia,Degang Zhao,Jianbo Zhang,Kunlun Li,Guoyin Kai

{"title":"Functional Characterization and Protein Engineering of Salvia miltiorrhiza Rosmarinic Acid Synthase.","authors":"Yue Feng,Zhoulu Wang,Junbo Li,Zhenli Ren,Yanting Wu,Hao Zhan,Xuefei Chen,Yao Wang,Min Shi,Xu Jia,Degang Zhao,Jianbo Zhang,Kunlun Li,Guoyin Kai","doi":"10.1021/acs.jafc.5c08090","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08090","url":null,"abstract":"Rosmarinic acid (RA) has been incorporated in various nutritious and health-promoting products. Rosmarinic acid synthase (RAS) is a committed enzyme in RA biosynthesis. However, SmRASs from Salvia miltiorrhiza remains functionally uncharacterized. Herein, a group of SmRASs was identified from the S. miltiorrhiza, and recombinant SmRAS1 was demonstrated to convert caffeoyl-CoA and 3,4-dihydroxyphenyllactic acid to form RA. SmRAS1 exhibited maximum activity at 45 °C and pH 8.0 with Km values of 18 μM and 1647 μM for caffeoyl-CoA and 3,4-dihydroxyphenyllactic acid, respectively. Subsequently, three highly catalytic activity SmRAS1 mutations R367W, G293I, and G293Rwere obtained, exhibiting 1.53-2.15 fold higher catalytic efficiency than the wild-type enzyme. Structural analysis and molecular dynamics simulations revealed that enlarged substrate access tunnel and reduced flexibility of residues286-295 located above the substrate pocket enhanced its catalytic activity. These results elucidate the mechanism underlying RA biosynthesis in S. miltiorrhiza and provide a promising RAS for RA biosynthesis in microbes.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"86 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptomyces-Induced 6-Nitrocoumarin Coordinates Rhizosphere Microbiome Recruitment for Pepper Blight Suppression. 链霉菌诱导的6-硝基香豆素协同根际微生物群募集抑制辣椒疫病。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c08807

Tianbing Zhou,Yuxin Xu,Yingtong Chen,Zhengbin Tang,Erxin Wang,Mengmeng Li,Mingrui Dou,Zhijia Zhang,Tao Zhang,Shujun Huang,Shuxian Gu,Shuai Wang,Ranfeng Sun,Dong Li

{"title":"Streptomyces-Induced 6-Nitrocoumarin Coordinates Rhizosphere Microbiome Recruitment for Pepper Blight Suppression.","authors":"Tianbing Zhou,Yuxin Xu,Yingtong Chen,Zhengbin Tang,Erxin Wang,Mengmeng Li,Mingrui Dou,Zhijia Zhang,Tao Zhang,Shujun Huang,Shuxian Gu,Shuai Wang,Ranfeng Sun,Dong Li","doi":"10.1021/acs.jafc.5c08807","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08807","url":null,"abstract":"Phytophthora capsici causes devastating losses in global pepper production. This study deciphers a sophisticated plant-microbe-metabolite collaboration initiated by rhizosphere-colonizing Streptomyces. We demonstrate that Streptomyces specifically primes pepper root biosynthesis of 6-nitrocoumarin, a nitrated coumarin derivative exhibiting potent broad-spectrum antioomycete activity (EC50: P. capsici 44.39 mg/L, Phytophthora palmivora 47.06 mg/L, Peronophythora litchii 34.92 mg/L). Crucially, this Streptomyces-recruited metabolite functions as a keystone rhizosphere signal, selectively enriching beneficial bacterial consortia while directly disrupting the ABC transporters of pathogens. The restructured microbiome synergistically amplifies plant systemic resistance through upregulating defense genes and enhancing root immunity. Our work establishes 6-nitrocoumarin as a dual-functional inoculant, a direct actinomycete compound and microbiome modulator, revealing a novel plant-microbe codefense paradigm driven by Streptomyces-induced metabolic rewiring for sustainable crop protection.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"161 2 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Nanomaterials for Cutting-Edge Biosensing of Foodborne Bacterial Pathogens 利用纳米材料对食源性细菌病原体进行尖端生物传感

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c05560

Subhashini Raj Kumal, Sun Tee Tay, Kwai Lin Thong, Jing Xuan Wong, Mohd Rafie Bin Johan, Suresh K. Bhargava, Bey Fen Leo

{"title":"Harnessing Nanomaterials for Cutting-Edge Biosensing of Foodborne Bacterial Pathogens","authors":"Subhashini Raj Kumal, Sun Tee Tay, Kwai Lin Thong, Jing Xuan Wong, Mohd Rafie Bin Johan, Suresh K. Bhargava, Bey Fen Leo","doi":"10.1021/acs.jafc.5c05560","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05560","url":null,"abstract":"The food industry represents a cornerstone of the global economy with continued expansion projected in the coming years. Ensuring food safety is paramount for safeguarding public health and supporting socio-economic prosperity. Despite stringent regulatory measures, foodborne illnesses caused by bacterial pathogens remain a significant concern. Conventional detection methods are often slow, labor-intensive, and insufficiently sensitive, limiting timely intervention to prevent outbreaks. There has been a concerted endeavor to develop point-of-care (POC) devices for the rapid and sensitive detection of foodborne pathogens. Nanomaterials (including metallic, nonmetallic, and inorganic substrates) with their unique physicochemical properties offer great potential for biosensing applications. This review summarizes recent advances in nanotechnology-based strategies for the POC detection of foodborne pathogens. This review highlights advances in nanotechnology-based biosensing platforms for POC applications, emphasizing the distinctive features of nanomaterials and their integration into innovative detection technologies aimed at enhancing food safety and reducing the burden of foodborne diseases.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"15 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "Genome-Wide Identification and Analysis of the JAZ Gene Family in Rapeseed Reveal JAZ2 and JAZ3 Roles in Drought and Salt Stress Tolerance". 更正“油菜JAZ基因家族的全基因组鉴定和分析揭示JAZ2和JAZ3在抗旱和耐盐胁迫中的作用”。

IF 6.2 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c11922

Peiyu Zhao, Jiaxin Zheng, Qiuyan Xu, Shengnan Zhao, Jie Li, Yaxuan Zhu, Jingli Yan, Qinqin Chen, Bo Yang, Chun Li, Feng Han, Yuan-Qing Jiang

引用次数: 0

Rational Engineering of Arginine Deiminase To Enhance the Acid Tolerance of Lactic Acid Bacteria. 精氨酸脱亚胺酶的合理工程设计提高乳酸菌的耐酸性。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c09676

Huan Yang,Liying Hao,Shangjie Yao,Rongqing Zhou,Chongde Wu

{"title":"Rational Engineering of Arginine Deiminase To Enhance the Acid Tolerance of Lactic Acid Bacteria.","authors":"Huan Yang,Liying Hao,Shangjie Yao,Rongqing Zhou,Chongde Wu","doi":"10.1021/acs.jafc.5c09676","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09676","url":null,"abstract":"In this work, the arginine deiminase system in lactic acid bacteria was activated in response to acid stress. Subsequently, a protein engineering strategy was rationally proposed to enhance the robustness of arginine deiminase, thereby improving the acid tolerance of lactic acid bacteria. Through molecular docking and molecular dynamics simulations, arginine deiminase mutants possessing a lower binding energy with the substrate arginine and higher stability (lower RMSD, RMSF, and Rg values compared with the wild type) were identified. Furthermore, the mutants were constructed in Lactococcus lactis NZ9000, and the resulting variants D71L, D120M, and D71L-D120M exhibited a higher enzymatic activity than the wild-type strain. The survival rate of L. lactis variants D71L, D120M, and D71L-D120M increased 15.07, 31.98, and 113.47%, respectively, compared with the wild-type strain under acid stress, suggesting the feasibility of rationally engineered arginine deiminase for improving the acid tolerance of lactic acid bacteria. Findings showed in this work may be beneficial for rationally improving the industrial functionalities of lactic acid bacteria during food biomanufacture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"21 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Methyltransferase Protein Encoded by Ustilaginoidea virens Polymycovirus 1 Suppresses Autophagy in Ustilaginoidea virens to Promote Viral Infection and Confer Host Hypovirulence. 毒菇多分枝病毒1编码的甲基转移酶蛋白抑制毒菇自噬促进病毒感染并赋予宿主低毒力。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c04009

Zhenrui He,Wenhua Zhao,Mei Yang,Baoping Cheng,Yiming Zhu,Yingqing Yang,Erxun Zhou

{"title":"The Methyltransferase Protein Encoded by Ustilaginoidea virens Polymycovirus 1 Suppresses Autophagy in Ustilaginoidea virens to Promote Viral Infection and Confer Host Hypovirulence.","authors":"Zhenrui He,Wenhua Zhao,Mei Yang,Baoping Cheng,Yiming Zhu,Yingqing Yang,Erxun Zhou","doi":"10.1021/acs.jafc.5c04009","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04009","url":null,"abstract":"Rice false smut (RFS), caused by the ascomycete fungus Ustilaginoidea virens (Cooke) Takahashi (teleomorph: Villosiclava virens), is a major panicle disease affecting rice production. Hypovirulence-associated mycoviruses represent promising biocontrol agents against plant fungal diseases. Here, we isolated a novel mycovirus, Ustilaginoidea virens polymycovirus 1 (UvPMV1), which alters the biological characteristics of the host fungus. Our findings demonstrate that UvPMV1 suppresses the autophagy pathway in U. virens and that autophagy is an essential defense mechanism during UvPMV1 infection. The methyltransferase (MT) protein encoded by UvPMV1 acted as an autophagy inhibitor and hypovirulence inducer by specifically interacting with the autophagy-related protein UvAtg6. Furthermore, transcriptomic and proteomic analyses revealed intricate virus-host interaction mechanisms. Collectively, we propose a model in which viral disruption of UvAtg6-mediated autophagy suppresses host defenses to enhance mycoviral proliferation. These findings present a promising biocontrol strategy against RFS and provide new insights into the agricultural application of mycoviruses.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"41 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Anthocyanin-Less 2 Diversity in Barley Reveals a Specific Allele to Cause Purple-Colored Grains. 大麦花青素- less 2多样性分析揭示一个导致紫色籽粒的特定等位基因。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c05032

Olesya Y Shoeva,Varvara D Zedgenizova,Anastasiya A Egorova,Sophia V Gerasimova,Tatjana V Kukoeva,Gennady V Vasiliev,Olga N Kovaleva,Shakhira Zakhrabekova,Mats Hansson,Christian W Hertig,Iris Hoffie,Jochen Kumlehn,Elena Khlestkina

{"title":"Analysis of Anthocyanin-Less 2 Diversity in Barley Reveals a Specific Allele to Cause Purple-Colored Grains.","authors":"Olesya Y Shoeva,Varvara D Zedgenizova,Anastasiya A Egorova,Sophia V Gerasimova,Tatjana V Kukoeva,Gennady V Vasiliev,Olga N Kovaleva,Shakhira Zakhrabekova,Mats Hansson,Christian W Hertig,Iris Hoffie,Jochen Kumlehn,Elena Khlestkina","doi":"10.1021/acs.jafc.5c05032","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05032","url":null,"abstract":"The purple color of the barley (Hordeum vulgare L.) grain is attributed to anthocyanins which are beneficial for human health. Synthesis of these pigments in both grain and vegetative tissues is controlled by ANT1 and ANT2 that belong to the families R2R3-MYB and bHLH of transcription factors, respectively. Here, we investigated the role of the Ant2 gene in pigmentation of grains compared to vegetative tissue. After screening of 504 barley accessions, six relevant alleles of Ant2 were identified. These involve distinct insertions in the promoter and/or in intron 6. Allele Ant2.l was found in all purple-grained barley accessions and was more strongly expressed compared to the alleles present in the other lines. The crucial role of Ant2.l in grain pigmentation was validated by targeted mutagenesis using RNA-guided endonuclease Cas9. It is further shown that the other Ant2 alleles do not affect grain pigmentation while being essential for pigmentation of vegetative tissues.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"25 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensing of Urea with a Functionalized Gold Electrode for Health and Food Monitoring. 功能化金电极尿素生物传感技术在健康和食品监测中的应用。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI: 10.1021/acs.jafc.5c08426

Angelo Ferlazzo,Meryam Chelly,Antonino Gulino,Giovanni Neri

引用次数: 0