功能化金电极尿素生物传感技术在健康和食品监测中的应用。

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-24 DOI:10.1021/acs.jafc.5c08426

Angelo Ferlazzo,Meryam Chelly,Antonino Gulino,Giovanni Neri

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引用次数: 0

摘要

生物体液中的尿素监测具有重要意义，因为尿素是肝脏和肾脏生理功能的关键指标，是血液动力学治疗的标志，并被用于食品掺假。因此，我们使用3,3'-二硫代二丙酸二（n-羟基琥珀酰亚胺酯）（DSP）双功能连接剂将脲酶（Ur）共价固定在丝网印刷金电极（SPGE）上，使脲酶和金电极之间实现最佳的电子通信，并开发了一种选择性尿素生物传感器（Ur-DSP/SPGE）。采用红外光谱（FTIR）、环伏安法（CV）和电阻抗谱（EIS）对Ur-DSP/SPGE进行了表征。采用开路电位法（OCP）检测水中不同浓度（0 ~ 600 μM）的尿素。该传感器尿素检测限（LOD）为5.0 μM，具有良好的时间稳定性和选择性。还对牛奶、人唾液和自来水中的尿素进行了检测，结果良好，回收率高。

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Biosensing of Urea with a Functionalized Gold Electrode for Health and Food Monitoring.

Urea monitoring in biological fluids is of crucial significance, since urea is a key indicator of liver and kidney physiological functioning, a marker for hemodynamic treatments, and has been used for food adulteration. Therefore, we have covalently anchored the urease enzyme (Ur) to a screen-printed gold electrode (SPGE) using the 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) bifunctional linker that allowed optimal electronic communication between urease and gold electrode and developed a selective urea biosensor (Ur-DSP/SPGE). The Ur-DSP/SPGE was characterized by infrared spectroscopy (FTIR), cyclovoltammetry (CV), and electrical impedance spectroscopy (EIS). Open-circuit potentiometry (OCP) was also used to detect urea at different concentrations (0-600 μM) in water. The biosensor showed a urea limit of detection (LOD) of 5.0 μM, as well as excellent temporal stability and selectivity. Urea sensing was also investigated in caw's milk, human saliva, and tap water with excellent results and recoveries.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.