Journal of Agricultural and Food Chemistry最新文献_第6页

Enhancing Chitosanase Activity via Directed Evolution and Its Molecular Mechanisms 定向进化增强壳聚糖酶活性及其分子机制

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-27 DOI: 10.1021/acs.jafc.5c08854

Yao Chen, Xiangyu Zi, Min Chen, Dengming Ming, Xiaolong Sun, Ling Jiang, Yongqian Fu

引用次数: 0

Citrinin Induces Hepatic Inflammatory Injury through the PERK-CHOP-NLRP3 Axis-Mediated Pyroptosis. 柠檬酸钠通过PERK-CHOP-NLRP3轴介导的焦亡诱导肝脏炎症损伤。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-27 DOI: 10.1021/acs.jafc.5c05538

Yongkang Wang,Yiya Feng,Bo Xiao,Jie Ni,You Wu,Qike Zhang,Xiaofang Liu,Shuiping Liu,Jine Yi,Zhihang Yuan,Jing Wu,Chenglin Yang

{"title":"Citrinin Induces Hepatic Inflammatory Injury through the PERK-CHOP-NLRP3 Axis-Mediated Pyroptosis.","authors":"Yongkang Wang,Yiya Feng,Bo Xiao,Jie Ni,You Wu,Qike Zhang,Xiaofang Liu,Shuiping Liu,Jine Yi,Zhihang Yuan,Jing Wu,Chenglin Yang","doi":"10.1021/acs.jafc.5c05538","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05538","url":null,"abstract":"Citrinin (CTN), a widespread food and feed contaminant, poses a significant health risk, yet its hepatic toxicity remains unclear. Here, we investigated the role of endoplasmic reticulum (ER) stress-mediated pyroptosis in CTN-induced liver injury using mice and HL-7702 cells. CTN exposure disrupted the hepatic cord structure, induced hepatocyte swelling with karyolysis, and promoted inflammatory infiltration. Liver injury markers and pro-inflammatory cytokines IL-1β and IL-18 were significantly elevated in both models, confirming inflammatory liver injury. Mechanistically, CTN activated pyroptosis-related proteins and triggered ER stress. In HL-7702 cells, CTN-induced inflammatory injury was mediated by NLRP3-dependent pyroptosis. Silencing CHOP alleviated injury by suppressing NLRP3 activation, while selective inhibition of PERK reduced CHOP expression and further attenuated pyroptosis. Collectively, these findings demonstrate that the PERK-CHOP pathway regulates NLRP3-dependent pyroptosis, contributing to CTN-induced hepatotoxicity. The PERK-CHOP-NLRP3 axis may represent a potential therapeutic target against CTN-related liver injury.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"92 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145153532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Glucosinolates and Alkaloids with Antibacterial Activities from Raphani Semen. Raphani子中具有抗菌活性的新型硫代葡萄糖苷类和生物碱。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-27 DOI: 10.1021/acs.jafc.5c11051

Meng-Qi Yan,Kang Yang,Ze-Jun Liu,Rui-Tong Du,Chang-Cheng Peng,Hai-Bo Wu,Sen Li,Zhen-Yue Wang,De-Qiang Yang,Li-Hong Wu,Meng Wang,Hai-Xue Kuang,Zhi-Bin Wang

{"title":"New Glucosinolates and Alkaloids with Antibacterial Activities from Raphani Semen.","authors":"Meng-Qi Yan,Kang Yang,Ze-Jun Liu,Rui-Tong Du,Chang-Cheng Peng,Hai-Bo Wu,Sen Li,Zhen-Yue Wang,De-Qiang Yang,Li-Hong Wu,Meng Wang,Hai-Xue Kuang,Zhi-Bin Wang","doi":"10.1021/acs.jafc.5c11051","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c11051","url":null,"abstract":"Sixteen compounds were isolated from Raphani Semen, including nine new glucosinolate compounds, raseglucosinolate A-I (1-9), four new alkaloids, rasemenine A-D (10-13), two known 4-methylthio-butyl derivatives (14, 15), and one known alkaloid (16). Compounds 1-13 were structurally characterized through a combination of HRESIMS, NMR, UV, IR, and ECD analyses. The potential antibacterial activities of selected compounds were evaluated. Compounds 3 and 10 exhibited inhibitory effects against Escherichia coli, with MIC of 32 and 64 μg/mL, respectively. Further mechanistic investigations demonstrated that 3 exerts its antibacterial activity by compromising the integrity of the cell wall and membrane, resulting in the leakage of intracellular contents and subsequent disruption of cellular metabolism and growth. Molecular docking analysis suggested that DNA gyrase and topoisomerase IV may be potential molecular targets of 3. Moreover, the combination of 3 with ampicillin sodium exhibited an additive antibacterial effect. These studies suggest that 3 has the potential to become an antibacterial agent and an antibiotic adjuvant.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"21 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145153536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of β-Glucan from Three Different Sources on Glucolipid Metabolism in Mice with Metabolic Syndrome. 三种不同来源β-葡聚糖对代谢综合征小鼠糖脂代谢的影响。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-27 DOI: 10.1021/acs.jafc.5c05443

Jingrui Yang,Huizi Tan,Song Li,Hong Yao,Qixing Nie,Steve W Cui,Shaoping Nie

{"title":"Effects of β-Glucan from Three Different Sources on Glucolipid Metabolism in Mice with Metabolic Syndrome.","authors":"Jingrui Yang,Huizi Tan,Song Li,Hong Yao,Qixing Nie,Steve W Cui,Shaoping Nie","doi":"10.1021/acs.jafc.5c05443","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05443","url":null,"abstract":"Glucan, a common dietary fiber, shows prebiotic potential for managing metabolic disorders, intestinal inflammation, and cardiovascular dysfunction. Although our earlier work found that glucans from various sources differ in molecular weight and linkage, how these variations affect their protective efficacy against metabolic syndrome (MetS) is still unknown. This study examined the effects of β-glucan from barley, highland barley, and oat bran on mice with high-fat diet-induced MetS. Various MetS-related indicators were analyzed, including overweight, dyslipidemia, hypertension, liver function, and host metabolites. Findings revealed that the differential effects of β-glucans primarily occurred in serum and hepatic lipid metabolism. HBBG was most effective at reducing LDL-C and hepatic lipid accumulation. Furthermore, changes in liver inflammation and lipid transport gene expression indicated that HBBG more effectively alleviated hepatic inflammation and regulated CCL2 and CD36 gene expression. These results highlight the source-dependent bioactivity of dietary β-glucans in improving metabolic disorders.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145153542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, Synthesis, and Aphicidal Activity of Novel Insect Neuropeptide Kinin Receptor Antagonists, Targeting the Ser2 Ligand Position. 针对Ser2配体位置的新型昆虫神经肽激肽受体拮抗剂的设计、合成和杀蚜活性研究。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-26 DOI: 10.1021/acs.jafc.5c05232

Jia-Wei Feng,Lu Wen,Kang-Li He,Li-Lin Luo,Shu-Zhong He,Guy Smagghe,Shun-Hua Gui,Tong-Xian Liu

{"title":"Design, Synthesis, and Aphicidal Activity of Novel Insect Neuropeptide Kinin Receptor Antagonists, Targeting the Ser2 Ligand Position.","authors":"Jia-Wei Feng,Lu Wen,Kang-Li He,Li-Lin Luo,Shu-Zhong He,Guy Smagghe,Shun-Hua Gui,Tong-Xian Liu","doi":"10.1021/acs.jafc.5c05232","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05232","url":null,"abstract":"Traditional chemical pesticides have raised significant environmental and health concerns, driving the pursuit of safer alternatives. Aphids, notorious for causing extensive agricultural damage and transmitting plant diseases, represent prime targets for next-generation insecticide development. This study investigates kinin neuropeptides, which regulate key physiological processes, as novel targets for biopesticide design. Using the kinin peptide as a structural lead, we synthesized 23 analogues and predicted their bioactivity via molecular docking and three-dimensional quantitative structure-activity relationship (3D-QSAR) modeling. Ser2 was identified as a critical site for enhancing receptor affinity. Two analogues, L02 and R01, emerged as potent antagonists of the aphid kinin G-protein-coupled receptor (GPCR), exhibiting strong binding in silico and functional inhibition in cell bioassays in vitro. Laboratory insect tests confirmed significant aphicidal activity, particularly for L02, which demonstrated superior efficacy. These findings highlight the potential of kinin-based GPCR antagonists as environmentally friendly biopesticides, offering target action with reduced nontarget impacts.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circannual Rhythms Affect the Bioavailability of Phenolic Compounds from Grape Seed Proanthocyanidins Extract Differently in Healthy and Obese Fischer 344 Rats. 周期节律对健康和肥胖Fischer 344大鼠葡萄籽原花青素提取物中酚类化合物生物利用度的影响不同

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-26 DOI: 10.1021/acs.jafc.5c03952

Iván Escobar-Martínez,Verónica Arreaza-Gil,Anna Arola-Arnal,Begoña Muguerza,Miquel Mulero,Manuel Suárez,Cristina Torres-Fuentes

{"title":"Circannual Rhythms Affect the Bioavailability of Phenolic Compounds from Grape Seed Proanthocyanidins Extract Differently in Healthy and Obese Fischer 344 Rats.","authors":"Iván Escobar-Martínez,Verónica Arreaza-Gil,Anna Arola-Arnal,Begoña Muguerza,Miquel Mulero,Manuel Suárez,Cristina Torres-Fuentes","doi":"10.1021/acs.jafc.5c03952","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03952","url":null,"abstract":"(Poly)phenols are plant-derived bioactive molecules that are associated with several health benefits. Therefore, it is essential to study the factors that affect the bioavailability of these phenolic compounds. Recently, circannual rhythms have been identified as one of the factors that may affect the bioavailability of these compounds. Hence, this study evaluates the impact of circannual rhythms on grape seed proanthocyanidins extract (GSPE) bioavailability in healthy and obesogenic contexts. Male Fischer 344 rats, fed standard (ST) or cafeteria (CAF) diets, were housed under different photoperiod conditions (6, 12, or 18 h of light per day) during 9 weeks and an oral dose of GSPE (25 mg/kg) was daily administered for the last 4 weeks. Serum GSPE-derived metabolites were then quantified by HPLC-ESI-MS/MS. A higher bioavailability was observed in rats exposed to a 12 h photoperiod and fed ST diet. However, this pattern was altered in CAF-fed rats, suggesting an attenuated influence of photoperiod under obesogenic conditions. These findings contribute to a better understanding of the complex relationships between diet, photoperiod, and serum metabolites.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substantial Progress and Remaining Challenges in Developing Low-Cadmium Rice in China. 中国低镉水稻发展的重大进展与挑战。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-26 DOI: 10.1021/acs.jafc.5c10019

Chengjing Liao,Min Huang

引用次数: 0

Akkermansia-Mediated BSH Regulation Alleviates Diabetic Dyslipidemia via the TCA-FXR/TGR5 Axis: Unraveling the Antidiabetic Mechanism of RKP. akkermansia介导的BSH调节通过TCA-FXR/TGR5轴缓解糖尿病血脂异常：揭示RKP的降糖机制

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-26 DOI: 10.1021/acs.jafc.5c05449

Zhouya Bai,Yunhui Zhang,Xiaojun Huang,Denglin Luo,Shaobin Gu,Kangyi Zhang,Shaoping Nie

引用次数: 0

Freeze-Thaw Cycles Accelerate Plastic Pollution Invasion in Agriculture: Trojan Horse Effect of Microplastic-Plasticizer Contamination Revealed in Rye via Computational Chemistry and Multiomics. 冻融循环加速塑料污染对农业的入侵：通过计算化学和多组学揭示黑麦中微塑料增塑剂污染的特洛伊木马效应。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c08155

Jinke Hu,Guozhang Bao,Jishun Wu,Simeng Chen,Yanan Xu,Huixin Wang,Lingzhi Tian,Zimin Fu,Ning Li,Yue Yuan

{"title":"Freeze-Thaw Cycles Accelerate Plastic Pollution Invasion in Agriculture: Trojan Horse Effect of Microplastic-Plasticizer Contamination Revealed in Rye via Computational Chemistry and Multiomics.","authors":"Jinke Hu,Guozhang Bao,Jishun Wu,Simeng Chen,Yanan Xu,Huixin Wang,Lingzhi Tian,Zimin Fu,Ning Li,Yue Yuan","doi":"10.1021/acs.jafc.5c08155","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08155","url":null,"abstract":"Climate change-related freeze-thaw (FT) cycles intensify combined contamination from diethyl phthalate (DEP) and microplastics (MPs) in agricultural systems. This study examines their synergistic phytotoxicity in hydroponic rye using multidisciplinary approaches, including physiology, transcriptomics, and computational modeling. FT dramatically increased cocontamination risks. Notably, DEP promoted MP uptake into plants by elevating MP surface charge─an effect intensified by FT─resulting in disrupted root function and nutrient absorption. Molecular analyses showed that DEP binds strongly to key proteins (e.g., HSP70; ΔG = -7.6 kcal/mol), inhibiting photosynthesis (net rate reduced by 20.1-64.5%) and altering antioxidant activity. MPs adsorbed DEP, reducing its mobility, while simultaneously reshaping the root microbiome to favor DEP-degrading bacteria (e.g., Rhizobium). Transcriptomic changes were observed in stress-responsive and phytohormone pathways. These results demonstrate that FT cycles exacerbate combined pollutant toxicity through surface charge modification, molecular disruption, and microbial community adaptation, offering important insights for assessing ecological risks under climate-induced stressors.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"93 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequence-Structure Combination Strategy Enables Discovery of a Novel Transglutaminase from Krasilnikovia cinnamomea and Computational Analysis. 序列-结构组合策略使得从Krasilnikovia cinnamomea发现一种新的转谷氨酰胺酶和计算分析。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-25 DOI: 10.1021/acs.jafc.5c07950

Wenjie Luo,Rui Xu,Shiwen Li,Zhaojun Wang,Maomao Zeng,Zhiyong He,Qiuming Chen,Jie Chen

{"title":"Sequence-Structure Combination Strategy Enables Discovery of a Novel Transglutaminase from Krasilnikovia cinnamomea and Computational Analysis.","authors":"Wenjie Luo,Rui Xu,Shiwen Li,Zhaojun Wang,Maomao Zeng,Zhiyong He,Qiuming Chen,Jie Chen","doi":"10.1021/acs.jafc.5c07950","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07950","url":null,"abstract":"Transglutaminase (TGase) is a widely utilized biocatalyst in the food industry, where it catalyzes the cross-linking of proteins in protein-rich foods, leading to improved texture and enhanced functional properties. Currently, commercially available TGases are derived from Streptomyces species. To expand the industrial application potential of TGase, this study employed a combined sequence- and structure-based strategy to identify a novel TGase from Krasilnikovia cinnamomea (KcTG). The enzyme was successfully heterologously expressed in Escherichia coli, exhibiting an optimal temperature of 60 °C and a maximum specific activity of 42.17 U/mg, outperforming SmTG. Furthermore, KcTG demonstrated efficient cross-linking of soy protein isolate, positioning it as a promising candidate for future industrial TGase enzyme preparations. This study also integrated computational tools to analyze the structural characteristics of KcTG based on surface net charge and hydrophobicity. Additionally, molecular dynamics (MD) simulations were employed to investigate its stability and catalytic performance.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"93 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0