Journal of Agricultural and Food Chemistry最新文献_第6页

Variety and Environmental Factors Affecting Pru p 7 Level in Peach Fruit: Implications for Low-Pru p 7 Germplasm Selection 影响桃果实prp7水平的品种和环境因素：对低prp7种质选择的启示

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-26 DOI: 10.1021/acs.jafc.6c09655

Bingyang Zhou, Weichao Fang, Xu Wang, Siqin Wang, Bingjie Chen, Yanmin Fan, Ke Cao, Yingpeng Hua, Jingyao Chen, Zhongshan Gao, Jing Jin

{"title":"Variety and Environmental Factors Affecting Pru p 7 Level in Peach Fruit: Implications for Low-Pru p 7 Germplasm Selection","authors":"Bingyang Zhou, Weichao Fang, Xu Wang, Siqin Wang, Bingjie Chen, Yanmin Fan, Ke Cao, Yingpeng Hua, Jingyao Chen, Zhongshan Gao, Jing Jin","doi":"10.1021/acs.jafc.6c09655","DOIUrl":"10.1021/acs.jafc.6c09655","url":null,"abstract":"Pru p 7 is a clinically important peach allergen associatedwithsevere allergic reactions, its quantitative variation and environmentaldeterminants remain poorly understood. Using a sensitive sELISA, wequantified Pru p 7 in 123 core peach accessions and assessed variationacross tissues, developmental stages, fruit traits, and environmentalconditions. Pru p 7 predominated in flesh (0.11–51.89 μg/gfresh weight (FW)), with extremely low levels in wild accessions (mean,0.20 μg/g FW). Its content varied among cultivars and was associatedwith flesh color, soluble solid content, aroma, and ripening date.May/June-ripening accessions averaged 21.15 μg/g FW, nearly6-fold higher than August-ripening accessions. Immunolocalizationand IgE immunoblotting supported these quantitative findings. Opaquebagging reduced Pru p 7, particularly in flesh. Three-day storageat 28 °C and 15-day storage at 4 °C also decreased levelsin most accessions. These results identify low-Pru p 7 germplasm andpractical strategies for allergen-risk management in peach production.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 43","pages":"34358–34368"},"PeriodicalIF":6.7,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149248681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bile Acid Sequestration by Soy Peptide Condensates Modulates the Digestion Fate of Soy 7S Protein Aggregates 大豆肽凝聚物对胆汁酸的固存调节大豆7S蛋白聚集体的消化命运

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 DOI: 10.1021/acs.jafc.6c09265

Tanghao Li,Kaining Han,Yunshan Wei,Ying Liu,Gaoshang Wang,Kunlun Liu,Xiaoquan Yang

{"title":"Bile Acid Sequestration by Soy Peptide Condensates Modulates the Digestion Fate of Soy 7S Protein Aggregates","authors":"Tanghao Li,Kaining Han,Yunshan Wei,Ying Liu,Gaoshang Wang,Kunlun Liu,Xiaoquan Yang","doi":"10.1021/acs.jafc.6c09265","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09265","url":null,"abstract":"The physiological functions of soy protein are commonly attributed to bioactive peptides released during digestion, whereas the roles of digestion-derived protein assemblies remain poorly understood. Here, we investigated two assemblies generated from soy 7S globulin, digestion-resistant core region (CR) aggregates and extension region (ER) peptide condensates. ER peptides formed condensates and remodeled CR aggregates from ordered amyloid-like structures into more disordered conformations. Bile acids promoted CR disassembly and proteolysis, whereas ER condensates sequestered bile acids and restricted their access to CR. Confocal imaging supported the spatial isolation of bile acids by ER condensates. Bile acids also enhanced epithelial transport and strengthened interactions between CR aggregates and mimetic lipid bilayers, whereas these effects were attenuated by ER-mediated bile acid sequestration. Our findings support a role for bile acid availability in modulating CR digestibility and transepithelial transport, linking peptide condensation, bile acid homeostasis, and protein functionality.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149359465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cell Wall-Associated Extracellular Catalase, PoCAT2, Confers Thermotolerance in Pleurotus ostreatus 细胞壁相关的胞外过氧化氢酶PoCAT2赋予平菇耐热性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 DOI: 10.1021/acs.jafc.6c09191

Chenxiao Zhou,Bingke Sun,Wenying Sun,Yanan Li,Haopeng Chai,Mehwish Buriro,Shichang Chen,Liyou Qiu,Yuqian Gao

{"title":"A Cell Wall-Associated Extracellular Catalase, PoCAT2, Confers Thermotolerance in Pleurotus ostreatus","authors":"Chenxiao Zhou,Bingke Sun,Wenying Sun,Yanan Li,Haopeng Chai,Mehwish Buriro,Shichang Chen,Liyou Qiu,Yuqian Gao","doi":"10.1021/acs.jafc.6c09191","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09191","url":null,"abstract":"Heat stress induces excessive extracellular H2O2 accumulation, compromising redox homeostasis and mushroom productivity. However, the role of extracellular catalases (CATs) in basidiomycetes remains poorly understood. Here, we identified PoCAT2, a cell wall-associated extracellular catalase in the basidiomycete Pleurotus ostreatus, whose secretion may involve a vesicle-mediated unconventional protein secretion pathway. Heat stress and exogenous H2O2 induced PoCAT2 expression and wall-associated catalase activity. Unlike intracellular PoCAT1, PoCAT2 overexpression increased catalase activity in both intracellular and cell wall compartments, enhancing in situ H2O2 scavenging and promoting mycelial growth under both optimal and elevated temperatures. Moreover, PoCAT2 overexpression accelerated fruiting and increased mushroom yield. These findings establish PoCAT2 as an extracellular antioxidant component that contributes to thermotolerance and fruiting performance in P. ostreatus, providing a potential strategy for improving thermotolerance in cultivated mushrooms.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"3 10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149359356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-Based Screening Combined with Functional Validation Identifies BtGSTs6-Mediated Neonicotinoid Detoxification in Bemisia tabaci 基于结构的筛选结合功能验证鉴定btgsts6介导的烟粉虱新烟碱解毒作用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 DOI: 10.1021/acs.jafc.6c09177

Rong Zhang,Fengbo Yang,Chao Wang,Mingrui Yuan,Xuegao Wei,Qimei Tan,Ying Zhang,Youjun Zhang,Qi Su,Xin Yang

{"title":"Structure-Based Screening Combined with Functional Validation Identifies BtGSTs6-Mediated Neonicotinoid Detoxification in Bemisia tabaci","authors":"Rong Zhang,Fengbo Yang,Chao Wang,Mingrui Yuan,Xuegao Wei,Qimei Tan,Ying Zhang,Youjun Zhang,Qi Su,Xin Yang","doi":"10.1021/acs.jafc.6c09177","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09177","url":null,"abstract":"Neonicotinoid resistance in the whitefly Bemisia tabaci causes substantial agricultural and economic losses worldwide. Although glutathione S-transferases (GSTs) are key phase II detoxification enzymes, their contribution to neonicotinoid resistance in this pest remains poorly defined. Here, we screened 23 GST genes against neonicotinoids using a “static screening + dynamic validation” strategy. Molecular docking and molecular dynamics simulations were used for candidate prioritization, followed by validation with RT-qPCR, RNAi, transgenic Drosophila, and UPLC-MS/MS. BtGSTs6 emerged as the principal candidate and was overexpressed in resistant populations. RNAi-mediated knockdown of BtGSTs6 increased susceptibility, whereas transgenic flies showed enhanced resistance to imidacloprid, thiamethoxam, and clothianidin. UPLC-MS/MS further confirmed that BtGSTs6 depleted the three insecticides to different extents, indicating substrate-dependent detoxification. These findings establish BtGSTs6 as a key mediator of neonicotinoid resistance and a promising target for resistance management and sustainable whitefly control.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149359382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Metabolomic and Proteomic Analyses Reveal Altered Iron Homeostasis and Immunometabolic Remodeling in the Spleen of Solea senegalensis during Vibriosis 综合代谢组学和蛋白质组学分析揭示了塞内加尔鳎在弧菌病期间脾脏铁稳态和免疫代谢重塑的改变

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-19 DOI: 10.1021/acs.jafc.6c02961

Larissa Buedenbender, Miguel Balado, Diego Rey-Varela, Marta A. Lages, Patricia Fernández Puente, Jaime Rodríguez, Manuel L. Lemos, Anthony R. Carroll, Carlos Jiménez

{"title":"Integrated Metabolomic and Proteomic Analyses Reveal Altered Iron Homeostasis and Immunometabolic Remodeling in the Spleen of Solea senegalensis during Vibriosis","authors":"Larissa Buedenbender, Miguel Balado, Diego Rey-Varela, Marta A. Lages, Patricia Fernández Puente, Jaime Rodríguez, Manuel L. Lemos, Anthony R. Carroll, Carlos Jiménez","doi":"10.1021/acs.jafc.6c02961","DOIUrl":"10.1021/acs.jafc.6c02961","url":null,"abstract":"Vibrio anguillarum isa major pathogenimpacting Solea senegalensis aquaculture.While host–pathogen competition for iron drives disease progression,the in vivo molecular evidence linking iron homeostasisand immune responses in fish remains limited. Here, we integratedmetabolomic and proteomic analyses to characterize the splenic responseof S. senegalensis during experimentalvibriosis. Multiomics integration revealed disruption of iron homeostasisalongside coordinated immunometabolic reprogramming. We detected thebacterial siderophore piscibactin directly in spleen tissue of soleexhibiting advanced stages of vibriosis, providing in vivo evidence of siderophore-mediated iron acquisition. Infection-drivenmetabolic shifts in lysine degradation, nucleotide metabolism, andmitochondrial energy pathways were correlated with heightened cellularproliferation and metabolic stress. Integrative analysis identifiedmetabolites including 2-aminoadipic acid, saccharopine, and methylsuccinicacid, together with iron-regulatory proteins (e.g., STEAP4) as candidatebiomarkers. These findings enhance our understanding of vibriosis-associatedimmunometabolic responses and provide a systems-level framework forfuture disease monitoring and management in aquaculture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 43","pages":"34468–34480"},"PeriodicalIF":6.7,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149218820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity-Guided Identification of Prot2633 as a Major Proteolytic Contributor to the Reduced Allergenicity of Lacticaseibacillus paracasei XJ-003-Fermented Milk 蛋白2633作为降低副干酪乳杆菌xj -003-发酵乳致敏性的主要蛋白水解因子的活性导向鉴定

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-22 DOI: 10.1021/acs.jafc.6c09711

Ruida Ma, Yunpeng Xu, Zihao Guo, Haiyun Zhang, Yiran Huang, Guangqing Mu, Xuemei Zhu

{"title":"Activity-Guided Identification of Prot2633 as a Major Proteolytic Contributor to the Reduced Allergenicity of Lacticaseibacillus paracasei XJ-003-Fermented Milk","authors":"Ruida Ma, Yunpeng Xu, Zihao Guo, Haiyun Zhang, Yiran Huang, Guangqing Mu, Xuemei Zhu","doi":"10.1021/acs.jafc.6c09711","DOIUrl":"10.1021/acs.jafc.6c09711","url":null,"abstract":"Lactic acid bacterial fermentation offers a food-compatiblestrategyfor reducing milk allergenicity, but the responsible proteolytic componentsremain poorly defined. We evaluated milk fermented with Lacticaseibacillus paracasei XJ-003 using pooledsera from milk-allergic donors and characterized its protease system.Activity-guided purification of cell pellet lysates yielded Prot2633,a native protease preparation dominated by S1 serine protease SP2685and S9 oligopeptidase SP1714. Prot2633 showed maximal activity atpH 6.5 and 43 °C and reduced IgE-binding reactivity of caseinand whey within defined hydrolysis windows. In sensitized BALB/c mice,XJ-003-fermented milk and Prot2633-hydrolyzed milk attenuated antibodyresponses, mast cell degranulation, Th2-associated inflammation, andintestinal barrier disruption, while producing treatment-associatedchanges in gut microbiota. These findings identify Prot2633 as animportant, but not exclusive, proteolytic contributor to the reducedallergenic reactivity of XJ-003-fermented milk and support activity-guideddevelopment of protease-assisted dairy processing.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 43","pages":"34285–34297"},"PeriodicalIF":6.7,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149230060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modular Pathway Engineering of an Escherichia coli Platform for De Novo Synthesis of Steviol Glycoside Precursors from Glucose 葡萄糖从头合成甜菊糖苷前体的大肠杆菌平台的模块化途径工程

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-22 DOI: 10.1021/acs.jafc.6c05347

Xuan Luo, Zheng-Sheng Shi, Wen-Xia Zheng, Shang-He Zheng, Hui-Dong Zheng, Qiang Guo, Li-Hai Fan

引用次数: 0

Licochalcone A Ameliorates Metabolic-Associated Fatty Liver Disease by AMPK Signaling Pathway Activation through O-GlcNAcylation Regulation and Remodeling of Gut Microbiota and Associated Metabolites 甘草查尔酮A通过o - glcn酰化调控和重塑肠道微生物群及相关代谢物激活AMPK信号通路，改善代谢性脂肪肝疾病

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-22 DOI: 10.1021/acs.jafc.6c04497

Shuai Wang, Shuai Guo, Xinjuan Wang, Hongwei Wang, Jicang Wang, Yanbo Ma, Shanshan Zhao, Cai Zhang

{"title":"Licochalcone A Ameliorates Metabolic-Associated Fatty Liver Disease by AMPK Signaling Pathway Activation through O-GlcNAcylation Regulation and Remodeling of Gut Microbiota and Associated Metabolites","authors":"Shuai Wang, Shuai Guo, Xinjuan Wang, Hongwei Wang, Jicang Wang, Yanbo Ma, Shanshan Zhao, Cai Zhang","doi":"10.1021/acs.jafc.6c04497","DOIUrl":"10.1021/acs.jafc.6c04497","url":null,"abstract":"Metabolic-associatedfatty liver disease (MAFLD) is amajor globalchronic liver disorder with serious health risks. Licorice-derivedlicochalcone A (LA) exhibits protective activities against multiplechronic conditions. Herein, we investigated the preventive and therapeuticeffects of LA on MAFLD. LA alleviated hepatic lipid accumulation,oxidative stress, and inflammation in MAFLD mice, and activated theAMPK pathway via O-GlcNAcylation. Furthermore, LA treatment restoredintestinal barrier function and reshaped gut microbiota, elevatingthe relative abundance of Muribaculaceae and Parasutterella. Gut microbiota depletion and FMT assaysdemonstrated that gut microbiota are indispensable for LA-mediatedMAFLD amelioration and hepatic O-GlcNAcylation regulation. Dodecanedioicacid (DA), a lipid-metabolism-related metabolite, improved MAFLD throughAMPK activation. In vitro assays confirmed that LA and DA inhibitedAMPK O-GlcNAcylation to boost AMPK phosphorylation and reduce cellularlipid deposition. These results offer new mechanistic perspectivesregarding the hepatoprotective properties of LA and confirm its therapeuticpotential to treat MAFLD.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 43","pages":"33853–33871"},"PeriodicalIF":6.7,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149229978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oleanolic Acid and Ursolic Acid Alleviate Obesity-Induced Skeletal Muscle Atrophy via Activation of the IRS/PI3K/AKT/mTOR Signaling Pathway and Attenuation of Oxidative Stress 齐墩果酸和熊果酸通过激活IRS/PI3K/AKT/mTOR信号通路和抑制氧化应激减轻肥胖诱导的骨骼肌萎缩

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-24 DOI: 10.1021/acs.jafc.6c04505

Quanrun He, Yishan Tong, Yue Yu, Zhichen Ren, Daozheng Fang, Zhihao Liu, Haimeng Li, Xinyue Li, Yong Zhu, Juanjuan Cheng, Jihang Chen

{"title":"Oleanolic Acid and Ursolic Acid Alleviate Obesity-Induced Skeletal Muscle Atrophy via Activation of the IRS/PI3K/AKT/mTOR Signaling Pathway and Attenuation of Oxidative Stress","authors":"Quanrun He, Yishan Tong, Yue Yu, Zhichen Ren, Daozheng Fang, Zhihao Liu, Haimeng Li, Xinyue Li, Yong Zhu, Juanjuan Cheng, Jihang Chen","doi":"10.1021/acs.jafc.6c04505","DOIUrl":"10.1021/acs.jafc.6c04505","url":null,"abstract":"Oleanolic acid (OA) and ursolic acid (UA) are dietarypentacyclictriterpenoids with anti-inflammatory and insulin-sensitizing properties,but their comparative effects on obesity-related skeletal muscle atrophyremain unclear. In this study, high-fat diet-fed C57BL/6J mice andpalmitic acid-treated C2C12 cells were used to evaluate their efficacyand mechanisms. OA and UA improved glucose homeostasis, attenuatedmuscle atrophy, and alleviated dyslipidemia and inflammation. Mechanistically,both compounds activated IRS/AKT/mTOR signaling, suppressed GSK-3β/FOXO1-mediatedcatabolism, alleviated endoplasmic reticulum stress, and regulatedautophagy balance. They also enhanced mitochondrial function and biogenesis.Transcriptomic and CMap analyses suggested PSMB1 as a potential target. In vitro, OA and UA improved mitochondrial respiration,ATP production, and glucose uptake. Overall, OA and UA effectivelymitigate HFD-induced muscle atrophy through coordinated regulationof insulin signaling, proteostasis, and mitochondrial function.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"74 43","pages":"33872–33887"},"PeriodicalIF":6.7,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149242000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Nano-Design to Field Deployment: An AI-Enabled Framework for Precision Agriculture 从纳米设计到现场部署：精准农业的人工智能支持框架

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-04 Epub Date: 2026-10-23 DOI: 10.1021/acs.jafc.6c07833

Anju Sangwan, Nitish Kumar, Neetu Singh

引用次数: 0