Journal of Agricultural and Food Chemistry最新文献_第6页

Structural Dynamics, Gut Microbiota Modulation, and Immunological Impacts of Shiitake Mushroom β-Glucan during In Vitro Intestinal Fermentation 香菇β-葡聚糖体外肠道发酵过程中的结构动力学、肠道菌群调节及免疫影响

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-01 DOI: 10.1021/acs.jafc.5c01611

Huanhuan Liu, Qiannan Wang, Lin Zeng, Yongshuai Liu, Yiheng Zhang, Zhenou Sun, Qingbin Guo, Steve W Cui

{"title":"Structural Dynamics, Gut Microbiota Modulation, and Immunological Impacts of Shiitake Mushroom β-Glucan during In Vitro Intestinal Fermentation","authors":"Huanhuan Liu, Qiannan Wang, Lin Zeng, Yongshuai Liu, Yiheng Zhang, Zhenou Sun, Qingbin Guo, Steve W Cui","doi":"10.1021/acs.jafc.5c01611","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01611","url":null,"abstract":"This study investigated the structure–function relationship of shiitake β-glucan (SBG) through simulated digestion and colonic fermentation. SBG, composed of β-(1 → 3)-linked glucan backbones with β-(1 → 6)-linked side chains, exhibited notable resistance to enzymatic and acidic degradation in the upper gastrointestinal tract. During in vitro colonic fermentation, 59% of carbohydrates were consumed within 48 h, with a significant pH reduction and a 1.5-fold increase in short-chain fatty acid production. Microbiome analysis demonstrated that SBG enhanced Bacteroides and Lactobacillus populations, while suppressing Escherichia-Shigella. Within the first 12 h, SBG maintained a rigid triple-helix structure, with a slight decrease in branching from 48.02 to 44.26%. After 24 h, the triple helix unwound, and extensive depolymerization of the backbone occurred by 48 h. Immunomodulatory activity was preserved early in fermentation but decreased as the triple-helix structure broke down. These findings emphasize the critical role of molecular rigidity and conformational integrity in β-glucan’s functionality for food and therapeutic applications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"49 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae) 夜蛾蛾性信息素合成相关去饱和酶Δ9和Δ11的鉴定（鳞翅目：夜蛾科）

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-01 DOI: 10.1021/acs.jafc.5c02139

Liuyang Wang, Pei Guo, Xiaofang Zhang, Yun Duan, Jun Ning, Tao Zhang, Xinling Yang

{"title":"Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae)","authors":"Liuyang Wang, Pei Guo, Xiaofang Zhang, Yun Duan, Jun Ning, Tao Zhang, Xinling Yang","doi":"10.1021/acs.jafc.5c02139","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02139","url":null,"abstract":"In moths, sex pheromones are synthesized in pheromone glands (PGs) by a variety of enzymes. Desaturases (DESs) are critical for the introduction of double bonds into pheromones. In Mythimna loreyi, the specific DESs involved in sex pheromone biosynthesis remain unclear. In this study, we identified and characterized 25 putative DESs from the M. loreyi genome. Nineteen of them were expressed in the female PGs, with seven showing significant upregulation in response to pheromone biosynthesis activating neuropeptide (PBAN). RNAi-based knockdown of MlorDES2 significantly reduced the titer of pheromone components by 58.6–85.9%, while knockdown of MlorDES9 specifically reduced the production of (Z)-9-tetradecenyl acetate. Functional verification in yeast revealed that MlorDES2 and MlorDES9 exhibited Δ11 and Δ9 desaturase activities, respectively. Taken together, these results collectively demonstrate that MlorDES2 and MlorDES9 are involved in sex pheromone biosynthesis of M. loreyi, suggesting that DESs could be used as potential targets for pest management.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"111 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting Two Odorant-Binding Proteins in Acyrthosiphon pisum: Discovery of Novel Jasmone Derivatives as Aphid Repellents 以两种气味结合蛋白为靶点：新型茉莉类杀虫剂的发现

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.4c12336

Ziwei Zhu, Huilin Li, Wenya Zhu, Shaoqing Du, Zhaokai Yang, Jianing Ren, Zhijian Xu, Hongxia Duan

{"title":"Targeting Two Odorant-Binding Proteins in Acyrthosiphon pisum: Discovery of Novel Jasmone Derivatives as Aphid Repellents","authors":"Ziwei Zhu, Huilin Li, Wenya Zhu, Shaoqing Du, Zhaokai Yang, Jianing Ren, Zhijian Xu, Hongxia Duan","doi":"10.1021/acs.jafc.4c12336","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12336","url":null,"abstract":"Odorant-binding proteins (OBPs) are potential targets for the discovery of insect behavior regulators. Cis-jasmone (CJ) was first identified as a repellent against Acyrthosiphon pisum (A. pisum) by its binding to ApisOBP3 and ApisOBP7. Therefore, twenty-one novel CJ derivatives were designed and synthesized based on the binding pockets of these two ApisOBPs. Almost all derivatives showed better binding affinities to ApisOBPs and aphid-repellent activities than CJ, respectively. The representative derivative 6bg had the most significant binding affinities to ApisOBP3 and ApisOBP7 with Ki values of 6.95 μM and 4.81 μM, respectively. Moreover, 6bg had the highest repellent rate of 71.62% against A.pisum at a dose of 5 μg and also elicited a dose-dependent electroantennography response. The binding mechanism revealed that hydrophobic interactions generated by nonpolar residues in ApisOBP3 and ApisOBP7 were key to the binding. This study offers insights into the rational design of novel aphid repellents targeting two OBPs.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De Novo Production of Piceatannol via Construction of Interconnected Biosynthetic Pathways in a Single Saccharomyces cerevisiae Strain 通过构建相互连接的生物合成途径在单个酿酒酵母菌株中从头生产杉木醇

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.5c01726

Jiazeng Yang, Zhuoxin Guo, Wenlong Zha, Jiachen Zi

引用次数: 0

Analysis of Coix Seed Oil Biosynthesis Facilitates the Identification of Lysophosphatidic Acid Acyltransferase 薏苡仁油生物合成分析有助于溶血磷脂酸酰基转移酶的鉴定

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.4c12507

Xiaoyan Wei, Jian Wang, Yuping Tan, Haxiu Zhu, Yan Wang, Shufeng Zhou, Juan Guo, Yanan Wang, Luqi Huang

{"title":"Analysis of Coix Seed Oil Biosynthesis Facilitates the Identification of Lysophosphatidic Acid Acyltransferase","authors":"Xiaoyan Wei, Jian Wang, Yuping Tan, Haxiu Zhu, Yan Wang, Shufeng Zhou, Juan Guo, Yanan Wang, Luqi Huang","doi":"10.1021/acs.jafc.4c12507","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12507","url":null,"abstract":"Coix seed oil (CSO) is a natural substance with significant anticancer potential. However, the molecular mechanism and the gene regulatory network of lipid biosynthesis were not identified in Coix seed. Here, a comprehensive transcriptome analysis was conducted on two Coix varieties with different lipid contents. The results revealed that a total of 48,110 genes were generated by de novo assembly, of which 84.45% genes were successfully annotated by the database. Based on functional annotation and gene expression, the metabolic network of crucial genes for oil accumulation and fatty acid (FA) synthesis in Coix seed has been successfully established, and it has been confirmed that lysophosphatidic acid acyltransferase (LPAT) in triacylglycerol (TAG) biosynthesis contributed to the changes in the content of FAs and had a significant selective binding ability to unsaturated fatty acids (UFAs) by the heterologous expression of yeast. Our data provides valuable references for subsequent gene function characterization and biosynthesis pathway optimization.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field Asymmetric Ion Mobility Spectrometry for Early Detection of Aphanomyces Root Rot in Peas Using Volatile Biomarkers 利用挥发性生物标志物进行豌豆隐匿菌根腐病的田间不对称离子迁移率测定

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.4c12571

Milton Valencia-Ortiz, Rebecca J. McGee, Sindhuja Sankaran

引用次数: 0

Metabolic Engineering for Resveratrol Production Based on Modularization of Metabolic Pathways in Escherichia coli 基于大肠杆菌代谢途径模块化的白藜芦醇生产代谢工程

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.5c00804

Daisuke Nonaka, Mayumi Kishida, Yuuki Hirata, Ayana Mori, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

{"title":"Metabolic Engineering for Resveratrol Production Based on Modularization of Metabolic Pathways in Escherichia coli","authors":"Daisuke Nonaka, Mayumi Kishida, Yuuki Hirata, Ayana Mori, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka","doi":"10.1021/acs.jafc.5c00804","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00804","url":null,"abstract":"Separating metabolic pathways for microbial chemical production is a promising approach for allocating resources based on target compounds. We modularized an artificial resveratrol biosynthetic pathway in Escherichia coli to supply p-coumaric acid and malonyl-CoA as precursors independently via the coutilization of glucose and xylose. The optimization of resveratrol synthetic gene expression, sugar concentration, and Corynebacterium glutamicum pyc overexpression improved the resveratrol titer in the engineered strain. Furthermore, introducing acetate into the malonyl-CoA supply module as a third carbon source resulted in an increased resveratrol titer. The cultivation of a strain overexpressing pyc and resveratrol synthetic genes in a 1-L bioreactor produced 273.5 mg/L resveratrol. The modularization of metabolic pathways to supply p-coumaric acid and malonyl-CoA from independent sources, such as sugars and acetate, enabled resveratrol production. Overall, this study contributes to the high production of chemicals whose biosynthetic pathways require multiple precursors such as polyketide.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"119 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoflavones and Stilbenes With Antifungal Activities From the Seeds of Thermopsis lanceolata R. Br 杉木种子中具有抗真菌活性的异黄酮和芪类化合物

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.5c00617

Cheng-Feng Liu, Jian-Guo Tan, Xing-Ya Wu, Cai-Yan Tian, Qiao An, Ping Yi, Xiao-Jiang Hao, Zhan-Xing Hu

{"title":"Isoflavones and Stilbenes With Antifungal Activities From the Seeds of Thermopsis lanceolata R. Br","authors":"Cheng-Feng Liu, Jian-Guo Tan, Xing-Ya Wu, Cai-Yan Tian, Qiao An, Ping Yi, Xiao-Jiang Hao, Zhan-Xing Hu","doi":"10.1021/acs.jafc.5c00617","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00617","url":null,"abstract":"In order to discover novel fungicides from natural products, the phytochemical investigation of Thermopsis lanceolata seeds was thoroughly conducted. Eight new isoflavone derivatives (1–5 and 8–10), two new stilbenes (6 and 7), as well as 21 known isoflavones (11–31) were obtained from the crude extract of T. lanceolata seeds following a bioassay-guided method. The structures of 1–31 were confirmed via HRESIMS, NMR, IR, UV, and quantum chemistry calculations. The antifungal activities of 1–31 against Phytophthora nicotiana, Colletotrichum orbiculare, Rhizoctonia solani, and Botrytis cinerea were screened according to the mycelial inhibition test. Compound 6 displayed significant antifungal activity against B. cinerea in vitro (EC50: 9.68 μg/mL) and had an 8 5.7% prevention and control effect in vivo (100 μg/mL). Moreover, antifungal mechanism studies revealed that 6 causes the accumulation of reactive oxygen species, increases the level of lipid peroxidation, decreases the activity of MDH, and finally triggers molecular damage and cell death in vitro. Therefore, 6 can be utilized as a lead compound for novel fungicides.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"56 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium Nanoparticles vs Selenite Fertilizers: Implications for Toxicological Profiles, Antioxidant Defense, and Ferroptosis Pathways 纳米硒与亚硒酸盐肥料：对毒理学、抗氧化防御和铁死亡途径的影响

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.5c02034

Qiting Fang, Zhonghua Liu, Kaixi Wang

{"title":"Selenium Nanoparticles vs Selenite Fertilizers: Implications for Toxicological Profiles, Antioxidant Defense, and Ferroptosis Pathways","authors":"Qiting Fang, Zhonghua Liu, Kaixi Wang","doi":"10.1021/acs.jafc.5c02034","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02034","url":null,"abstract":"Selenium (Se) foliar fertilizers enhance crop nutrition and address human selenium deficiency, while improper application may lead to excessive intake and residue accumulation. Our study comprehensively assessed the toxicity and function of novel selenium nanoparticles and traditional sodium selenite fertilizers across cell, zebrafish, and murine models. Both fertilizers enhanced antioxidant pathways at low doses, but selenium nanoparticles exhibited stronger antioxidant and ferroptosis-modulating effects with lower toxicity at a high dose. Sodium selenite increased total and lipid ROS production, leading to decreased viability of cells and increased distortion and mortality of zebrafish. In mice, sodium selenite induced hepatic toxicity and decreased GPX4. Transcriptome analysis revealed that sodium selenite downregulated c-JUN and APOA4, weakening the antioxidant defense, whereas selenium nanoparticles promoted ferroptosis resistance through FGF21. These findings suggest selenium nanoparticles as a safer alternative for Se biofortification, mitigating health risks while supporting food security and environmental sustainability.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"87 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PuERF27-PuMYB10-PuGSTF12 Module Regulates Melatonin-Induced Anthocyanin Biosynthesis in Pear Fruits PuERF27-PuMYB10-PuGSTF12模块调控褪黑素诱导的梨果花青素生物合成

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-04-30 DOI: 10.1021/acs.jafc.5c01317

Huili Sun, Xinyue Wang, Guangfu Gu, Miao Zhang, Xiaoqian Wang

{"title":"The PuERF27-PuMYB10-PuGSTF12 Module Regulates Melatonin-Induced Anthocyanin Biosynthesis in Pear Fruits","authors":"Huili Sun, Xinyue Wang, Guangfu Gu, Miao Zhang, Xiaoqian Wang","doi":"10.1021/acs.jafc.5c01317","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01317","url":null,"abstract":"Anthocyanins, flavonoids with strong antioxidant activity, give rise to a fruit peel color and provide health benefits. Studies have revealed that melatonin promotes anthocyanin accumulation. However, the function of endogenous hormones in this process remains elusive. Here, we investigated the effect of ethylene signaling on anthocyanin accumulation in melatonin-treated pear fruit. Melatonin promoted anthocyanin accumulation while repressing ethylene production; its effect on anthocyanin biosynthesis in the background of ethephon pretreatment was eliminated. Transcriptome analysis revealed PuERF27, a melatonin-induced ethylene response factor (ERF), as a candidate gene. Transient overexpression of PuERF27 is essential for enhancing anthocyanin accumulation and activating anthocyanin biosynthesis genes, whereas PuERF27 silencing repressed melatonin-induced anthocyanin accumulation. Yeast one-hybrid, electrophoretic mobility shift, and GUS assays indicated that PuERF27 could bind directly to and positively regulate the PuMYB10 and PuGSTF12 promoters. Further studies revealed that PuERF27, together with PuMYB10, synergistically transactivates the PuGSTF12 promoter. These results indicate that melatonin-induced PuERF27 may directly promote PuGSTF12 expression and indirectly activate structural genes through PuMYB10, thereby leading to the biosynthesis and transport of anthocyanins.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"46 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0