Journal of Agricultural and Food Chemistry最新文献_第6页

Cynarin TargetsHost CX3CR1 for Dual-Action Prophylaxisagainst RSV Infection and Pulmonary Pathology Cynarin TargetsHost CX3CR1双作用预防RSV感染和肺部病理

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c04350

Yingjie Qing,Jiaqing Liu,Wei Hong,Luhua Zou,Lu Zhong,Peng Cao

{"title":"Cynarin Targets\u0000Host CX3CR1 for Dual-Action Prophylaxis\u0000against RSV Infection and Pulmonary Pathology","authors":"Yingjie Qing,Jiaqing Liu,Wei Hong,Luhua Zou,Lu Zhong,Peng Cao","doi":"10.1021/acs.jafc.6c04350","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c04350","url":null,"abstract":"Abstract Safe, small-molecule prophylactic agents against respiratory syncytial virus (RSV) are critically lacking. Here, we identify Cynarin, an artichoke-derived dietary polyphenol, as a micromolar affinity CX3CR1 antagonist. Mechanistically, Cynarin confers dual-action protection through two distinct routes: it acts as a “chemical shield” to competitively block viral entry at the epithelial interface by binding CX3CR1, and through a separate CX3CR1-independent mechanism, exhibits intrinsic, virus-independent anti-inflammatory properties by directly suppressing macrophage activation. This target-based interaction was rigorously validated via biochemical and functional assays. In an RSV-infected mouse model, prophylactic administration of Cynarin demonstrated superior efficacy over therapeutic treatment. It significantly reduced pulmonary viral load, ameliorated lung consolidation, and potently suppressed the RSV-induced cytokine storm. Collectively, our findings establish a novel host-directed strategy utilizing Cynarin to target CX3CR1, highlighting the translational potential of food-derived bioactives in offering a dual prophylactic safeguard against viral infection and associated pulmonary pathology.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato Trichomesas Metabolic Cell Factories: SpecializedMetabolism, Developmental Regulation, and Defense Function 番茄毛状体：代谢细胞工厂：特殊代谢、发育调节和防御功能

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c04409

Di He,Hongyu Chen,Shixi Gao,Peng Wang,Darren C. J. Wong,Shan Lu,Haiyang Xu,Fei Zhou

{"title":"Tomato Trichomes\u0000as Metabolic Cell Factories: Specialized\u0000Metabolism, Developmental Regulation, and Defense Function","authors":"Di He,Hongyu Chen,Shixi Gao,Peng Wang,Darren C. J. Wong,Shan Lu,Haiyang Xu,Fei Zhou","doi":"10.1021/acs.jafc.6c04409","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c04409","url":null,"abstract":"Abstract Tomato trichomes are dynamic interfaces for plant–environment interactions, serving as primary physical barriers against external stressors and as specialized cell factories for diverse metabolites. These highly specialized epidermal structures synthesize and sequester terpenoids, acylsugars, and methyl ketones, constructing a multidimensional defense line against herbivores and pathogens through physical entrapment and chemical repellency. Owing to their complex multicellular architecture, trichome morphogenesis and specialized metabolism are coordinated by intricate genetic regulatory networks. The SlWoolly-centered transcriptional complex, together with effector factors such as SlMYC1 and Hair, links cellular differentiation with substrate metabolic flux, rendering them an ideal model for studying the integration of cell fate determination and metabolic regulation. This review integrates current knowledge of specialized-metabolite biosynthesis, developmental regulation, and physiological functions in tomato trichomes, providing a framework for improving stress resistance and biomanufacturing potential through molecular-design breeding and for exploiting trichomes as controllable platforms for specialized-metabolite production.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"86 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineeringof Saccharomyces cerevisiae for Flavonoid Synthesis from Acetate 醋酸合成类黄酮的酿酒酵母代谢工程研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c05419

Mei Li,Guangjian Li,Yutao Wang,Wei Xie,Xin Zhang,Canyu Zhang,Yufei Cao,Wen-Yong Lou

{"title":"Metabolic Engineering\u0000of\u0000 Saccharomyces cerevisiae\u0000 for Flavonoid Synthesis from Acetate","authors":"Mei Li,Guangjian Li,Yutao Wang,Wei Xie,Xin Zhang,Canyu Zhang,Yufei Cao,Wen-Yong Lou","doi":"10.1021/acs.jafc.6c05419","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c05419","url":null,"abstract":"Abstract Acetate is a sustainable C2 feedstock derived from CO2 electroreduction, but its conversion to complex natural products is constrained by impaired cell growth and poor metabolic compatibility. Adaptive laboratory evolution generated an evolved Saccharomyces cerevisiae strain capable of producing 536.32 mg/L of p-coumaric acid from 15 g/L acetate as the sole carbon source. Genome- and transcriptome-level analyses subsequently revealed genetic factors associated with an enhanced acetate utilization. To evaluate acetate-supported flavonoid biosynthesis, a downstream flavonoid pathway was constructed in the parental chassis, yielding 19.55 mg/L of phloretin from acetate. Co-overexpression of EFG1 and ACS1, followed by fermentation optimization, increased the phloretin titer to 35.71 mg/L, reaching 78.71% of the glucose-based level. This work establishes S. cerevisiae as a viable platform for converting renewable C2 feedstocks into complex flavonoids and provides a practical strategy for the low-carbon bioproduction of high-value natural products.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"68 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EnantioselectiveNeurotoxic Risk of Ethiprole Exposureto Honeybees: Prediction of Multidimensional Mechanisms 乙丙醇暴露于蜜蜂的对映体选择性神经毒性风险：多维机制的预测

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c07615

Yonghong Zhang,Ziyu Chen,Yujia Wang,Keyi Ouyang,Xiaobo Wu

{"title":"Enantioselective\u0000Neurotoxic Risk of Ethiprole Exposure\u0000to Honeybees: Prediction of Multidimensional Mechanisms","authors":"Yonghong Zhang,Ziyu Chen,Yujia Wang,Keyi Ouyang,Xiaobo Wu","doi":"10.1021/acs.jafc.6c07615","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c07615","url":null,"abstract":"Abstract Limited research on the sublethal effects and mechanisms of chiral pesticides, particularly ethiprole, on honeybee neurobehavior at the enantiomeric level has obscured our understanding of the survival risks facing honeybees. We used transcriptomic sequencing, behavioral assay, correlation analysis, and molecular modeling to investigate the enantioselective neurotoxic risk of ethiprole on honeybees and predict potential molecular mechanisms. Our findings indicate that S-ethiprole poses a significant neurotoxicity hazard by markedly disrupting multiple signaling pathways and altering the expression of more genes, primarily by targeting the Rho subfamily of the small GTPase superfamily. This disruption likely accounts for the impairment of the gustatory response behavior in honeybees. Furthermore, Rac protein emerges as a potential molecular target through which S-ethiprole influences signal transduction in honeybees, correlating with decreased Snodgrassella abundance and the accumulation of N-benzoyl-N′-(2-chlorophenyl)thiourea induced by S-ethiprole. These results contribute to understanding the enantioselective neurotoxic risks and mechanisms of chiral ethiprole on honeybees.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"73 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Engineering of Carboxylic Acid Reductasefor Improved Catalytic Efficiency and Substrate Tolerance EnablingGreen Vanillin Biosynthesis 合理设计羧酸还原酶以提高催化效率和底物耐受性，实现绿色香兰素生物合成

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c11041

Hanyu Zhu,Longzheng Huang,Ximeng Liu,Yiyang Zhang,Wei-Bing Liu,Bang-Ce Ye

{"title":"Rational Engineering of Carboxylic Acid Reductase\u0000for Improved Catalytic Efficiency and Substrate Tolerance Enabling\u0000Green Vanillin Biosynthesis","authors":"Hanyu Zhu,Longzheng Huang,Ximeng Liu,Yiyang Zhang,Wei-Bing Liu,Bang-Ce Ye","doi":"10.1021/acs.jafc.6c11041","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11041","url":null,"abstract":"Abstract Vanillin is a high-value natural aromatic compound widely used in food, flavor, and pharmaceutical fields. Carboxylic acid reductase (CAR)-catalyzed vanillic acid bioreduction offers a green vanillin biosynthesis route, yet low catalytic activity and severe substrate inhibition limit its industrial application. Herein, we screened 10 bacterial CAR homologues and constructed superior chimeric CAR templates via domain recombination. Combined with structure-guided directed evolution and substrate tunnel remodeling, the optimized quadruple mutant M4 was obtained, exhibiting 4-fold higher catalytic efficiency compared with the wild-type MavCAR (and 1.6-fold higher than the parental chimera M0) and partially relieved activity decline at elevated substrate concentrations. Structural simulations clarified that tunnel expansion and hydrogen bond remodeling synergistically stabilized enzyme–substrate binding. The M4-based whole-cell system achieved 95% vanillin conversion (8.7 g·L–1) from 10 g·L–1 vanillic acid, and a two-step cascade system realized efficient de novo vanillin synthesis from glucose. This work provides a robust engineered CAR biocatalyst for green vanillin preparative-scale biosynthesis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PPARγ/P21Signaling Mediates Butyrate-DrivenProtection against Aging-Related Endothelial Dysfunction: A NovelEffect of Soybean Polysaccharide via the Gut-Vascular Axis PPARγ/ p21信号介导丁酸盐驱动的抗衰老相关内皮功能障碍：大豆多糖通过肠-血管轴的新作用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c10019

Qile Zhang,Zhengming Zhou,Hoi Wa Yeung,Yiu Ming Cheung,Yuen Lam Wong,Chufeng He,Shouhe Huang,Lu Jiang,Xiao Yu Tian,Zhen-Yu Chen,Wing Tak Wong

{"title":"PPARγ/P21\u0000Signaling Mediates Butyrate-Driven\u0000Protection against Aging-Related Endothelial Dysfunction: A Novel\u0000Effect of Soybean Polysaccharide via the Gut-Vascular Axis","authors":"Qile Zhang,Zhengming Zhou,Hoi Wa Yeung,Yiu Ming Cheung,Yuen Lam Wong,Chufeng He,Shouhe Huang,Lu Jiang,Xiao Yu Tian,Zhen-Yu Chen,Wing Tak Wong","doi":"10.1021/acs.jafc.6c10019","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c10019","url":null,"abstract":"Abstract Aging-induced endothelial dysfunction is a core driver of age-related cardiovascular diseases (CVDs). In 20-month-old naturally aged mice, 12-week soybean polysaccharide (SP) supplementation restored endothelium-dependent relaxation, reduced aortic reactive oxygen species and VCAM-1 in the aortic endothelium, and attenuated colon senescence, improved gut barrier integrity, and reduced systemic inflammation, as shown by decreased serum LPS, IL-1β, TNF-α, and fecal and serum TMA/TMAO levels. SP enriched butyrate-producing gut bacteria, including Lachnospiraceae_NK4A136_group, and increased gut and serum butyrate, consistent with enhanced microbial short-chain fatty acid biosynthesis capacity predicted by PICRUSt2. Direct butyrate supplementation reproduced these vascular and colonic benefits. It activated PPARγ signaling and downregulated p21, ICAM-1, VCAM-1, and p16, whereas PPARα and PPARδ remained unchanged. These findings support a gut microbiota–butyrate–PPARγ/p21 axis through which SP protects against aging-related endothelial dysfunction, providing a nutritional basis for targeting the gut–vascular axis in cardiovascular aging.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"17 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-Reactivitybetween Scylla paramamosain and Dermatophagoides farinae : Identificationof Tropomyosin and Arginine Kinase as Major Cross-Reactive Allergens 原肌球蛋白和精氨酸激酶作为主要交叉反应性变应原的鉴定

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c11421

Fei Xia,Mengsi Li,Qingmei Liu,Liqiang Zhong,Yubao Cui,Chih-Jung Chang,Feng Lin,Yixiang Liu,Guangming Liu

{"title":"Cross-Reactivity\u0000between\u0000 Scylla paramamosain\u0000 and\u0000 Dermatophagoides farinae\u0000 : Identification\u0000of Tropomyosin and Arginine Kinase as Major Cross-Reactive Allergens","authors":"Fei Xia,Mengsi Li,Qingmei Liu,Liqiang Zhong,Yubao Cui,Chih-Jung Chang,Feng Lin,Yixiang Liu,Guangming Liu","doi":"10.1021/acs.jafc.6c11421","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11421","url":null,"abstract":"Abstract Scylla paramamosain and Dermatophagoides farinae are dominant allergen sources in Asia, yet the molecular basis of their cross-reactivity remains poorly defined. Among 385 HDM-positive sera, 13.5% exhibited IgE reactivity to S. paramamosain extract, demonstrating serological cosensitization between crustacean and mite in southeastern China. Four homologous allergen pairs, namely, tropomyosin (TM), arginine kinase (AK), troponin C (TnC), and triosephosphate isomerase (TIM), shared &gt;50% sequence identity and were all cross-recognized by IgG on Western blot. Basophil activation testing nevertheless distinguished the pairs functionally, as TM and AK activated basophils in 95% and 88% of cross-reactive patients, whereas TnC and TIM did not reach statistical significance at the group level. AK, conventionally ranked below TM in crustacean allergy, therefore, contributes almost equally to cross-reactivity. Cross-inhibition ELISA further demonstrated directional asymmetry in IgE cross-reactivity, and eight and nine conserved B-cell epitope regions were mapped on TM and AK. These findings clarify the allergen basis of crab-mite cosensitization and support AK-inclusive component-resolved diagnosis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organophosphate Esters and Their Metabolites in Commercially Available Preprepared Dishes: Occurrence, Migration from Food Contact Materials, and Dietary Exposure. 市售的预制盘子中的有机磷酸酯及其代谢物：发生、从食品接触材料迁移和饮食暴露。

IF 6.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c08095

Minghao Sun, Sai Fan, Wei Liu, Jiaying Liu, Jing Zhan, Zhixiong Shi

{"title":"Organophosphate Esters and Their Metabolites in Commercially Available Preprepared Dishes: Occurrence, Migration from Food Contact Materials, and Dietary Exposure.","authors":"Minghao Sun, Sai Fan, Wei Liu, Jiaying Liu, Jing Zhan, Zhixiong Shi","doi":"10.1021/acs.jafc.6c08095","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08095","url":null,"abstract":"<p><p>Organophosphate esters (OPEs) are used as flame retardants and plasticizers and may enter preprepared dishes through raw materials, processing, and migration from food contact materials (FCMs). In this study, 28 OPEs and 13 mOPEs were determined in 347 commercially available preprepared dish samples purchased in Beijing, China, and migration experiments were conducted using 42 independent FCM samples. OPEs were widely detected in preprepared dishes (median: 9.04 ng/g wet weight (ww)), while mOPEs had much lower levels (median of 1.40 ng/g ww). Compared with fresh animal-origin foods, the dishes showed significantly higher OPE concentrations. Independent migration experiments further showed that several major OPEs could be released from the tested FCMs, demonstrating release potential consistent with a possible packaging contribution. A scenario-based exposure assessment indicated that OPE intake via preprepared dish consumption was below available health-based guidance values, while uncertainties remain because consumption data specific to preprepared dishes and toxicological thresholds for many OPEs/mOPEs are lacking.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprocessing ofShrimp Shell Waste into Chitin andFunctional Ingredients with High Bioactive Activity 虾壳废弃物生物加工制备几丁质及高生物活性功能性成分的研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c09636

Jariya Ruangwicha,Benjamas Cheirsilp,Wasana Suyotha

引用次数: 0

Ameliorative Effectsof Casein Peptides on Constipation-PredominantIrritable Bowel Syndrome: Involvement of the GC-C/cGMP Signaling Pathway 酪蛋白肽对便秘为主的抗便秘性肠综合征的改善作用：GC-C/cGMP信号通路的参与

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-15 DOI: 10.1021/acs.jafc.6c00778

Zhiqing Lin,Yingjie Wang,Ying Su,Jiayi Li,Jian He,Sufang Duan,Wenyi Zhang,Xuebo Liu,Long Ding

{"title":"Ameliorative Effects\u0000of Casein Peptides on Constipation-Predominant\u0000Irritable Bowel Syndrome: Involvement of the GC-C/cGMP Signaling Pathway","authors":"Zhiqing Lin,Yingjie Wang,Ying Su,Jiayi Li,Jian He,Sufang Duan,Wenyi Zhang,Xuebo Liu,Long Ding","doi":"10.1021/acs.jafc.6c00778","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c00778","url":null,"abstract":"Abstract This study investigated the effects of casein peptides on constipation-predominant irritable bowel syndrome (IBS-C) in mice. Results revealed that oral administration of casein hydrolyzate significantly attenuated constipation symptoms, reduced visceral hypersensitivity, improved intestinal barrier integrity, suppressed inflammatory responses, and restored serum neurotransmitter balance in IBS-C mice. Casein hydrolyzate did not alter guanylyl cyclase C (GC-C) mRNA levels but significantly increased cyclic guanosine-3‘,5′-monophosphate (cGMP) levels in the colon of IBS-C mice, suggesting the possible involvement of the GC-C/cGMP signaling pathway in the above effects. Additionally, nine casein peptides with potential GC-C agonistic activity were identified through an integrated in silico screening and ex vivo functional evaluation, among which PFPGPIPN showed the highest activity. Moreover, in vivo experiments confirmed that PFPGPIPN significantly ameliorated IBS-C symptoms and elevated colonic cGMP levels in mice. These findings highlight the potential of casein peptides as bioactive ingredients in functional foods for the management of IBS-C.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0