Journal of Agricultural and Food Chemistry最新文献_第5页

Structure-Guided Evolution of Curcumin Reductase for Sustainable Tetrahydrocurcumin Biosynthesis. 四氢姜黄素生物合成中姜黄素还原酶的结构引导进化

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-30 DOI: 10.1021/acs.jafc.5c07455

Jiaying Hao,Yunju Zhang,Kaiwei Liu,Boyu Zhang,Hao Wang,Yuning Jin,Bin Wang,Di Wang,Ying Li,Xueli Wei,Qinyuan Ma,Xiuzhen Gao

{"title":"Structure-Guided Evolution of Curcumin Reductase for Sustainable Tetrahydrocurcumin Biosynthesis.","authors":"Jiaying Hao,Yunju Zhang,Kaiwei Liu,Boyu Zhang,Hao Wang,Yuning Jin,Bin Wang,Di Wang,Ying Li,Xueli Wei,Qinyuan Ma,Xiuzhen Gao","doi":"10.1021/acs.jafc.5c07455","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07455","url":null,"abstract":"Tetrahydrocurcumin (THC), a major bioactive metabolite of curcumin, exhibits superior bioavailability and stability, making it a highly sought-after ingredient for nutraceutical and functional food applications. Current chemical and microbial THC production methods both suffer from selectivity issues and require complex purification steps. Here, we developed an enzymatic platform for THC biosynthesis through systematic enzyme discovery and engineering. We mined and identified a novel enzyme from Kosakonia cowanii with a good soluble expression. Structure-guided design yielded a triple mutant, M3 (K274L/P266F/Q286Y), with an 8.14-fold enhanced catalytic efficiency. Molecular dynamics simulations and binding free energy analysis revealed that mutations synergistically enhanced substrate binding and optimized the catalytic environment through both local conformational changes and intersubunit interactions. In a preparative-scale synthesis, M3 achieved 99.7% conversion of 50 mM curcumin within 3 h, yielding 17.56 g/L of THC. This enzymatic approach eliminates toxic metals and complex purification procedures, providing a sustainable alternative for industrial THC production.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"99 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Novel Flexible β-Ketonitrile Derivatives Bearing a Biphenyl Moiety as Potential Succinate Dehydrogenase Inhibitors. 含有联苯基团的新型柔性β-酮腈衍生物作为琥珀酸脱氢酶抑制剂的发现。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-30 DOI: 10.1021/acs.jafc.5c08208

Liangliang Cheng,Sihui Wang,Cong Zhou,Na Zhai,Biao Wu,Zhong Li,Jiagao Cheng

{"title":"Discovery of Novel Flexible β-Ketonitrile Derivatives Bearing a Biphenyl Moiety as Potential Succinate Dehydrogenase Inhibitors.","authors":"Liangliang Cheng,Sihui Wang,Cong Zhou,Na Zhai,Biao Wu,Zhong Li,Jiagao Cheng","doi":"10.1021/acs.jafc.5c08208","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c08208","url":null,"abstract":"Plant pathogenic fungi pose a significant threat to global agriculture, highlighting an urgent need for the development of novel fungicides due to resistance issues. A series of pyrazole-β-ketonitrile compounds incorporating a biphenyl structure were designed and synthesized. Notably, compound A37 demonstrated superior potency against Rhizoctonia solani, with an EC50 value of 0.0144 μg/mL, more potent than the commercial fungicide fluxapyroxad (EC50 = 0.0270 μg/mL). In vivo assays demonstrated that A37 exhibited good protective activity against rice sheath blight. Enzyme inhibition activity assays revealed that A37 was a potent inhibitor of succinate dehydrogenase (SDH), with an IC50 value of 0.0263 μM. Docking studies within R. solani SDH indicated that A37 effectively binds into the ubiquinone site mainly through hydrogen-bond and cation-π and π-π interactions. The identified optimal pyrazole-β-ketonitrile compound A37, containing a biphenyl structural motif, served as a potent lead for further structural optimization as a potential agricultural SDH inhibitor fungicide.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"32 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational-Guided Protein Engineering of LaACS Coupled with SAM Synthase Screening Synergistically Boosts ACC Production in E. coli. 计算导向的LaACS蛋白工程结合SAM合成酶筛选协同促进大肠杆菌ACC的产生

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-30 DOI: 10.1021/acs.jafc.5c05832

Yupeng Wan,Long Jiang,Yaojie Liu,Zongjiang Yu,Mo Xian,Chao Xu

{"title":"Computational-Guided Protein Engineering of LaACS Coupled with SAM Synthase Screening Synergistically Boosts ACC Production in E. coli.","authors":"Yupeng Wan,Long Jiang,Yaojie Liu,Zongjiang Yu,Mo Xian,Chao Xu","doi":"10.1021/acs.jafc.5c05832","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05832","url":null,"abstract":"1-Aminocyclopropane-1-carboxylic acid (ACC) is a nonprotein amino acid commonly found in plants, serving as an ethylene precursor with independent hormone functions that regulate plant growth, development, and stress resistance. Due to its agricultural significance, efforts have focused on boosting its production. In this study, ACC biosynthesis was achieved in E. coli via LaACS (1-aminocyclopropane-1-carboxylate synthase) from Lycoris aurea, which catalyzes S-adenosylmethionine (SAM). To enhance the yield, structural prediction of LaACS using AlphaFold2 and MOE docking identified the mutant LaACSL243A, which exhibited a doubled Vmax (0.018 mM/L/min) and Kcat (12.033 min-1) and 20% higher Kcat/Km (15.88 mM-1 min- 1) than that of the wild type. The intrinsic reasons for the enhanced catalytic efficiency were elucidated through molecular dynamics simulation. Subsequently, through screening of S-adenosylmethionine synthase (SAMS), SAMSa (from Arabidopsis thaliana) was identified as having the highest catalytic activity. Overexpression of the genes encoding SAMSa, MetA (homoserine O-succinyltransferase), and MetH (methionine synthase) successfully increased the ACC yield to 109 mg/L in 50 mL of M9 medium, which is twice that of the unmodified strain. The development of a green, safe, and efficient ACC biosynthetic process is of significant importance for promoting the application of ACC in agricultural science, biology, and medicine.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"26 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Antiphotoaging Peptides from Bovine Ligamentum Nuchae Proteins Hydrolysates: Based on Sprague-Dawley Rat Model and Molecular Docking. 基于Sprague-Dawley大鼠模型和分子对接的新型牛颈韧带蛋白水解物抗光老化肽研究。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-30 DOI: 10.1021/acs.jafc.5c09950

Caihong Li,Jiaxin He,Ruiqi Wu,Luyun Cai,Wuyin Weng,Mouming Zhao,Defeng Xu

{"title":"Novel Antiphotoaging Peptides from Bovine Ligamentum Nuchae Proteins Hydrolysates: Based on Sprague-Dawley Rat Model and Molecular Docking.","authors":"Caihong Li,Jiaxin He,Ruiqi Wu,Luyun Cai,Wuyin Weng,Mouming Zhao,Defeng Xu","doi":"10.1021/acs.jafc.5c09950","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09950","url":null,"abstract":"This study identified novel antiphotoaging peptides from bovine ligamentum nuchae proteins hydrolysates (BLPs) and clarified their molecular mechanisms in photoaging SD rats. Initially, BLPs effectively alleviated photoaging manifestations. Biochemically, BLPs significantly reduced the oxidative stress and suppressed the phosphorylation levels of p38, JNK, and ERK in the mitogen-activated protein kinase pathway. Simultaneously, BLPs dramatically downregulated the hyperactivation of the nuclear factor kappa B pathway and reduced the inflammatory cytokines accordingly (P < 0.05). Moreover, two peptide fragments with the highest antioxidant capacity in separated fractions were identified with ultraperformance liquid chromatography-tandem mass spectrometry, with the sequences of Gly-Leu-Pro-Tyr and Gly-Pro-Gly-Gly-Val-Gly-Ala-Leu. Subsequently, molecular docking revealed that the identified peptides established strong binding affinity to the four oxidation-sensitive cysteine residues in the active sites of MMP-1 mainly by hydrogen bonding and hydrophobic interactions. Collectively, this study demonstrates that BLPs exert potent photoprotection by synergistically targeting oxidative stress and MMP-1 active sites.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"18 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the Interaction Mechanism of Protocatechuate 3,4-Dioxygenase with Substrates via Multispectroscopy and Molecular Docking. 基于多光谱和分子对接的原儿茶酸3,4-双加氧酶与底物相互作用机制研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-29 DOI: 10.1021/acs.jafc.5c05630

Jiliang Cheng,Huan Du,Shan-Shan Jiang,Xiao-Jie Liu,Nian-Jue Zheng,Jin-Cheng Ye,Dina Zhang,Kun Xin,Lei Xiang,Yan-Wen Li,Quan-Ying Cai,Ce-Hui Mo,Hai-Ming Zhao

{"title":"Insights into the Interaction Mechanism of Protocatechuate 3,4-Dioxygenase with Substrates via Multispectroscopy and Molecular Docking.","authors":"Jiliang Cheng,Huan Du,Shan-Shan Jiang,Xiao-Jie Liu,Nian-Jue Zheng,Jin-Cheng Ye,Dina Zhang,Kun Xin,Lei Xiang,Yan-Wen Li,Quan-Ying Cai,Ce-Hui Mo,Hai-Ming Zhao","doi":"10.1021/acs.jafc.5c05630","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05630","url":null,"abstract":"Protocatechuic acid (PCA) is a key intermediate in aromatic pollutant biodegradation, and protocatechuate 3,4-dioxygenase (3,4-PCD) can efficiently cleave its aromatic ring. The interaction mechanism remains incompletely understood. This study investigated the interaction mechanism between PCA and a 3,4-PCD from Rhodococcus sp. 2G. The recombinant enzyme was heterologously expressed in Escherichia coli, yielding 2.7 mg/L with high catalytic efficiency. Fluorescence quenching confirmed static quenching and efficient complex formation. Multispectral analysis revealed conformational and microenvironmental changes upon PCA binding. Integrated molecular dynamics simulations and site-directed mutagenesis identified Tyr253, Tyr337, His389, His391, and Arg386 as synergistic residues for PCA ring cleavage, with hydrogen bonds and electrostatic interactions being crucial. A refined catalytic mechanism was proposed. These findings provide deep biophysical insights into 3,4-PCD/PCA interactions, advance mechanistic knowledge of dioxygenases in aromatic catabolism, and offer a basis for enzyme engineering to reduce pollutants in agricultural and food systems.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"6 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional and Functional Insights into GmPUB33A-Mediated Regulation of Soybean Resistance to Soybean Cyst Nematode. gmpub33a介导的大豆对大豆囊肿线虫抗性调控的转录和功能研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-29 DOI: 10.1021/acs.jafc.5c05847

Nawei Qi,Hongxin Liu,Wenshu Kang,Jichen Yan,Yuxi Duan

{"title":"Transcriptional and Functional Insights into GmPUB33A-Mediated Regulation of Soybean Resistance to Soybean Cyst Nematode.","authors":"Nawei Qi,Hongxin Liu,Wenshu Kang,Jichen Yan,Yuxi Duan","doi":"10.1021/acs.jafc.5c05847","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05847","url":null,"abstract":"Ubiquitination-related genes are pivotal in modulating plant responses to biotic stress. The U-box E3 ubiquitin ligase gene GmPUB33A has been identified as a negative regulator of resistance to soybean cyst nematode (SCN). To elucidate the underlying molecular mechanisms, we conducted high-throughput sequencing and transcriptome analyses on GmPUB33A-overexpression and -RNAi transgenic hairy roots. Three differentially expressed genes (DEGs) were silenced using siRNA for functional validation. Compared with empty vector control, 11,088 DEGs were identified. Gene Ontology (GO) and KEGG analyses revealed these DEGs were significantly enriched in defense responses, oxidative stress, phenylpropanoid biosynthesis, and MAPK signaling. Functional analysis revealed that silencing Glyma.07G061500 and Glyma.19G258700 reduced the number of nematodes by 32.62% and 19.34%, respectively. Furthermore, β-glucuronidase (GUS) activity and reactive oxygen species (ROS) assays demonstrated their involvement in SCN parasitism and ROS accumulation. These findings provide novel insights into GmPUB33A-mediated SCN susceptibility in soybean.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"100 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermostable Xylanase from Chenopodium album: Thermodynamic and Kinetic Characterization with Sustainable Application in Agro-Industrial Biobleaching. Chenopodium中耐热木聚糖酶的热力学和动力学表征及其在农业工业生物漂白中的可持续应用。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-29 DOI: 10.1021/acs.jafc.5c05447

Khalil Ur Rehman,Ehab A Abdelrahman,Karma M Albalawi,Mohammed Alissa,Abdullah Alghamdi,Suad A Alghamdi,Alaa S Alhegaili,Mohammed A Alshehri,Mortaga M Abou-Krisha,Abdulrahman G Alhamzani

{"title":"Thermostable Xylanase from Chenopodium album: Thermodynamic and Kinetic Characterization with Sustainable Application in Agro-Industrial Biobleaching.","authors":"Khalil Ur Rehman,Ehab A Abdelrahman,Karma M Albalawi,Mohammed Alissa,Abdullah Alghamdi,Suad A Alghamdi,Alaa S Alhegaili,Mohammed A Alshehri,Mortaga M Abou-Krisha,Abdulrahman G Alhamzani","doi":"10.1021/acs.jafc.5c05447","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c05447","url":null,"abstract":"This study focused on the purification and complete biochemical and thermodynamic characterization of a thermostable xylanase derived from Chenopodium album. The enzyme exhibited a molecular weight of approximately 48 kDa and retained 85% activity after 2 h at 50 °C and pH 5.0, indicating substantial thermal and pH stability. Kinetic analysis revealed a Km of 0.26 mM and a Vmax of 33 μmol min-1 mg-1, confirming high substrate affinity and catalytic efficiency. Thermodynamic analysis revealed enhanced conformational stability of the enzyme, characterized by elevated enthalpy and Gibbs free energy values. The observed negative entropy change (ΔS° = -22.87 J mol-1 K-1 at 70 °C) indicates increased molecular ordering during thermal denaturation, particularly in the presence of sorbitol, which acted as a stabilizing polyol, enhancing the conformational rigidity of the enzyme. The enzyme's biotechnological relevance was further validated through kraft pulp biobleaching trials. Treatment with 100 U/g of xylanase led to a 32% reduction in the Kappa number, an increase in brightness from 38.5% to 51.2% ISO, and minimal losses in viscosity (-4.8%) and yield (95.6%), confirming selective delignification without compromising cellulose integrity. These findings underscore the enzyme's promise in reducing chemical load during pulp biobleaching and efficiently producing xylo-oligosaccharides, which possess recognized prebiotic and nutraceutical value.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-17A: A Bright Target for Toxicological Mechanisms and Mitigation of Toxic Elements in the Environment. 白细胞介素- 17a：环境中有毒元素的毒理学机制和缓解的一个光明目标。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-28 DOI: 10.1021/acs.jafc.5c09000

Qing Xiang,Yunxiao Zhou,Xiang Li,Lijuan Zhang,Jianhai Zhang

{"title":"Interleukin-17A: A Bright Target for Toxicological Mechanisms and Mitigation of Toxic Elements in the Environment.","authors":"Qing Xiang,Yunxiao Zhou,Xiang Li,Lijuan Zhang,Jianhai Zhang","doi":"10.1021/acs.jafc.5c09000","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09000","url":null,"abstract":"Science and other peer-reviewed studies recognize cadmium (Cd), arsenic (As), mercury (Hg), and fluoride (F) as highly hazardous toxic elements that threaten global health. However, understanding their toxic mechanisms and effective mitigation remains a global challenge. Growing evidence suggests that interleukin-17A (IL-17A) not only plays a role in inflammatory and immune diseases but may also be the brightest target in the mechanisms of toxic injury and its mitigation. This review summarizes IL-17A signaling in toxic element poisoning, highlighting distinct effects on apoptosis, autophagy, and pyroptosis: fluorine promotes cell death, cadmium shows dose-dependent regulation of IL-17A-mediated apoptosis, and arsenic and mercury may inhibit pyroptosis and IL-17A secretion. Moreover, interventions such as riboflavin, bifidobacterium, melatonin (MLT), Ginkgo biloba extract (GBE), lentinan, and taxifolin, were discussed for their protective effects via IL-17A modulation. This provides novel insights into IL-17A signaling, health risks, and therapeutic approaches against toxic elements.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"96 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Administration of Ovalbumin Prevents Food Allergy via Improving Microbiota and Metabolic Disorders in a Dose-Dependent Manner. 口服卵清蛋白通过改善微生物群和代谢紊乱以剂量依赖的方式预防食物过敏。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-28 DOI: 10.1021/acs.jafc.5c09864

Shan Zhang,Qiang Shi,Yuhong Wu,Xiao Chen,Jingjing Li,Pingduo Chen,Yong Wu,Xin Li,Shuangyan Zheng,Jie Zhang,Zhongliang Wang,Hongbing Chen

{"title":"Oral Administration of Ovalbumin Prevents Food Allergy via Improving Microbiota and Metabolic Disorders in a Dose-Dependent Manner.","authors":"Shan Zhang,Qiang Shi,Yuhong Wu,Xiao Chen,Jingjing Li,Pingduo Chen,Yong Wu,Xin Li,Shuangyan Zheng,Jie Zhang,Zhongliang Wang,Hongbing Chen","doi":"10.1021/acs.jafc.5c09864","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09864","url":null,"abstract":"Food allergy (FA), triggered by specific dietary allergens, is a growing global concern regarding public health and food safety. Our previous studies suggest that early exposure to certain allergenic proteins can lower FA risk, but the dose-response relationship remains unclear. In this study, BALB/c mice received 0.1-10 mg of ovalbumin (OVA) by gavage before sensitization. 0.1 mg of OVA failed to prevent FA, with a diarrhea incidence of 75%. In contrast, 1 mg of OVA provided partial protection, reducing the diarrhea incidence to 25%, while 5 or 10 mg of OVA fully prevented allergic reactions. Besides, higher OVA doses better suppressed Th2 polarization and increased tolerogenic cDC1 cell frequencies. Importantly, 5 mg of OVA restored gut microbial diversity and ameliorated metabolic dysregulation, while lower doses showed no significant improvement compared to the FA group. Overall, the OVA pretreatment prevents FA in a dose-dependent manner by modulating gut microbiota and metabolism.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Protein Engineering and Expression Balancing Enable Efficient d-Allulose Production. 整合蛋白工程和表达平衡使d-Allulose高效生产。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-28 DOI: 10.1021/acs.jafc.5c09894

Xue Cai,Cheng-Hao Hu,A-Yang Wu,Yan-Feng Yan,Xin-Yue Fang,Xin-Yan Lu,Chang-Hui Hu,Wulong Yang,Hong-Yan Wang,Xiaojian Zhang,Li-Qun Jin,Mian Li,Zhi-Qiang Liu,Yu-Guo Zheng

{"title":"Integrated Protein Engineering and Expression Balancing Enable Efficient d-Allulose Production.","authors":"Xue Cai,Cheng-Hao Hu,A-Yang Wu,Yan-Feng Yan,Xin-Yue Fang,Xin-Yan Lu,Chang-Hui Hu,Wulong Yang,Hong-Yan Wang,Xiaojian Zhang,Li-Qun Jin,Mian Li,Zhi-Qiang Liu,Yu-Guo Zheng","doi":"10.1021/acs.jafc.5c09894","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09894","url":null,"abstract":"Allulose, a rare sugar with promising applications in food and health industries, can be efficiently synthesized via biocatalysis using d-allulose 3-epimerase (DAE). However, challenges in enzyme thermostability and cascade efficiency limit industrial scalability with low-cost substrates. Here, we engineered a thermostable DAE from Clostridium cellulolyticum through semirational design, yielding a triple mutant (F155Y/D281G/C289R) with a 12.0 °C increase in melting temperature (Tm to 73.2 °C), optimal temperature shifted to 60 °C, and a conversion rate improved from 27.5 to 33.3%─surpassing reported rates. Integrated with a modular coexpression system for expression-level balancing between DAE and thermophilic glucose isomerase, our one-pot cascade achieved high d-allulose titers from glucose (18.5%), F42 fructose syrup (17.4%), and corncob hydrolysate (7.5%)─outperforming literature benchmarks in yield and sustainability. This work advances green biomanufacturing by converting agricultural residues into high-value sugars, offering a scalable alternative to traditional methods with reduced costs and enhanced process continuity.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"96 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0