Journal of Agricultural and Food Chemistry最新文献

{"title":"Rutin Synergizes with Colistin to Eradicate Salmonellosis in Mice by Enhancing the Efficacy and Reducing the Toxicity","authors":"Yang Luo, Bing He, Zhi-Peng Li, Qin Zhong, Yu-Chen Liu, Hai-Yi Zhang, Yan Li, Hui-Lin Yan, Ya-Lin Hu, Zi-Jian Zheng, Hao Ren, Xiao-Ping Liao, Jian Sun","doi":"10.1021/acs.jafc.4c06751","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06751","url":null,"abstract":"The wide dissemination of multidrug-resistant (MDR) Gram-negative bacteria poses a significant global health and security concern. As developing new antibiotics is generally costly, fastidious, and time-consuming, there is an urgent need for alternative therapeutic strategies to address the gap in antibiotic discovery void. This study aimed to investigate the activity of colistin (CS) in combination with a natural product, rutin (RT), to combat against <i>Salmonella</i> Typhimurium (<i>S.</i> Tm) in vitro and in vivo. The results showed that a combination with RT enabled the potentiation of CS efficacy. Further mechanistic analysis indicated that RT disrupted iron homeostasis to inactivate the PmrA/PmrB system, thereafter reducing the bacterial membrane modifications for enhancing CS binding. Besides enhancing bactericidal activity of CS, RT was also observed to mitigate the CS-induced nephrotoxicity, by which the dosing limitation of CS was overcome for better pathogen clearance. The animal trial eventually confirmed the in vivo synergistic interaction of RT with CS to treat the bacterial infection. To sum up, the present study uncovered the potential of RT as a viable adjuvant of CS to eradicate the infection and protect the hosts, which might serve as a promising alternative to combat infections caused by MDR Gram-negative bacteria.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"88 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constructing High-Yielding Serratia marcescens for (−)-α-Bisabolol Production Based on the Exogenous Haloarchaeal MVA Pathway and Endogenous Molecular Chaperones","authors":"Yao Lu, Di Liu, Long Wang, Yongai Ma, Tai-Ping Fan, Huaxiang Deng, Yujie Cai","doi":"10.1021/acs.jafc.4c10135","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10135","url":null,"abstract":"(−)-α-Bisabolol exhibits analgesic, anti-inflammatory, and skin-soothing properties and is widely applied in the cosmetic and pharmaceutical industries. The use of plant essential oil distillation or chemical synthesis to produce (−)-α-bisabolol is both inefficient and unsustainable. Currently, the microbial production of (−)-α-bisabolol mainly relies on <i>Escherichia coli</i> and <i>Saccharomyces cerevisiae</i> as chassis strains; however, high concentrations of (−)-α-bisabolol have certain toxicity to the strain. This study uses synthetic biology and metabolic engineering strategies to redesign a solvent-tolerant <i>Serratia marcescens</i> for the efficient production of (−)-α-bisabolol. By introducing the Haloarchaea-type mevalonate (MVA) pathway and the (−)-α-bisabolol biosynthesis pathway, we successfully constructed a strain capable of producing (−)-α-bisabolol. The coexpression of the chaperone protein DnaK/J significantly enhanced the soluble expression of the (−)-α-bisabolol synthase, resulting in a 10% increase in (−)-α-bisabolol titer. Furthermore, knockout of the <i>PhoA</i> gene, which reduced the formation of the byproduct farnesol (FOH), further increased the (−)-α-bisabolol titer to 3.21 g/L. In a 5 L bioreactor, <i>S. marcescens</i> achieved a final (−)-α-bisabolol titer of 30.2 g/L, representing the highest titer reported to date. This research provides guidance for the production of (−)-α-bisabolol in nonmodel microorganisms without the requirement for induction.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"89 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Talarergosteroids A–C: Three Unusual Steroid-Polyketone Conjugates with Antifungal Activity from a Kandelia Obovata Derived Fungus Talaromyces sp","authors":"Jialin Li, Zirong Lin, Haiqi Zeng, Jiechang Zeng, Siyao Ye, Chen Chen, Hao Jia, Kang Li, Zhigang She, Yuhua Long","doi":"10.1021/acs.jafc.4c10156","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10156","url":null,"abstract":"Three previously undescribed steroid–polyketone conjugates, talarergosteroids A–C (<b>1</b>–<b>3</b>), together with talarergosteroid D (<b>4</b>), which was first identified from a natural source, were isolated from a <i>Kandelia Obovata</i> derived fungus <i>Talaromyces</i> sp. SCNU-F0041. Compounds <b>1</b> and <b>2</b> bear a complicated 6/6/6/5/6/6 hexacyclic ring system characterized by an oxaspiro[5.5]undecane architecture. Compound <b>3</b> possesses a benzofuran moiety substituted at C-3 in ergosterol. The structures of the new compounds were identified by comprehensive spectroscopic analysis, X-ray diffraction, and electronic circular dichroism (ECD) calculation. Talarergosteroid B (<b>2</b>) showed significant inhibitory activity against the agricultural plant pathogen <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> (MIC = 0.78 μg/mL), outperforming the positive control carbendazim (MIC = 1.56 μg/mL). Preliminary research disclosed that compound <b>2</b> may inhibit the spore germination progress, malform the fungal mycelium, and damage the organelle. These results indicate that compound <b>2</b> could be a potential fungicidal lead compound against <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i>.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Amino Acid Mutation in Cytochrome P450 (CYP96A146) of Descurainia sophia on the Metabolism and Resistance to Tribenuron-Methyl","authors":"Jing Shen, Qian Yang, Fan Xu, Yuxin Han, Yubin Li, Mingqi Zheng","doi":"10.1021/acs.jafc.4c10217","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10217","url":null,"abstract":"Cytochrome P450 monooxygenases (P450s) play important roles in herbicide resistance. In this study, there are four amino acid mutations (F39Y, H163Y, S203A, and V361E) between <i>CYP96A146-S</i> and <i>CYP96A146-R</i>, which were cloned, respectively, from susceptible (S) and tribenuron-methyl-resistant (TR) <i>Descurainia sophia</i>. The Arabidopsis expressing <i>CYP96A146-S</i> or <i>CYP96A146-R</i> showed resistance to tribenuron-methyl, carfentrazone-ethyl, and oxyfluorfen, while Arabidopsis transformed with CYP96A146-R or CYP96A146 with any two or three mutations of H163Y, S203A, or V361E exhibited significantly higher resistance to tribenuron-methyl than Arabidopsis expressing CYP96A146-S. The metabolic rates of tribenuron-methyl were significantly faster in Arabidopsis expressing <i>CYP96A146-R</i> than that with <i>CYP96A146-S</i>. The molecular dynamics simulation demonstrated that amino acid mutations did not affect the domain of the HEM ring, which could significantly enhance the volume of the catalytic pocket in P450 (CYP96A146), thereby increasing the collision rate between the catalytic pocket and tribenuron-methyl. Hence, the amino acid mutations may be one of the mechanisms underlying P450-mediated herbicide resistance.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"54 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, Synthesis of Dienthiazole Derivatives, and Evaluation of Aphicidal Activity.","authors":"Yuming Ma, Yuxiao Hu, Weibin Dong, Qiangping Wang, Jinyan Wang, Wenjun Wu, Baojun Shi","doi":"10.1021/acs.jafc.4c06060","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06060","url":null,"abstract":"<p><p>Nitrogen-containing heterocycles have attracted attention for the development of chemicals because of their many types, high physiological activities, and ease of synthesis. Aphids are severe pests found worldwide that cause serious losses in crop yield and quality every year. In this study, a series of novel dienolone thiazole derivatives were synthesized using dienolone acetate as the parent molecule. The synthesis involved bromination, Hantzsch reaction, esterification, deprotection, and other reactions. The structure of the compounds was determined using proton and carbon-13 nuclear magnetic resonance, high-resolution mass spectrometry, and single-crystal diffraction. The synthesized compounds exhibited excellent insecticidal activities against five species of aphids, including <i>Schizaphis graminum</i>, <i>Brevicoryne brassicae</i>, <i>Aphis gossypii</i>, <i>Aphis citricola</i> Van der, and <i>Myzus persicae</i>. The median lethal concentration values of the compound <b>H-13</b> for <i>S. graminum</i>, <i>B. brassicae</i>, <i>A. gossypii</i>, <i>A. citricola</i> Van der, and <i>M. persicae</i> were 8.72, 13.77, 14.17, 12.96, and 12.35 μg/mL, respectively. The mode of action test results indicated that compound <b>H-13</b> had superior contact and systemic activity against <i>M. persicae</i>, similar to the positive control flonicamid. Furthermore, a field trial showed that the control effect of compound <b>H-13</b> at 100 μg/mL concentration was comparable to that of flonicamid against <i>M. persicae</i>. The mortality was 85.6% and 90.3% after 7 and 14 days, respectively. Finally, to further explore the action mechanism of these compounds, the insecticidal activity of compounds <b>H-13</b> (strong) and <b>H-24</b> (weak) on aphid protease was determined. Compound <b>H-13</b> was found to have a significant inhibitory effect on the strong alkaline tryptase activity. Compound <b>H-13</b> might cause aphid poisoning and death by inhibiting the trypsin activity. This study provided important insights for the discovery and development of new insecticides.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucoidan-Mediated Covalent Modification Mitigates Allergenicity of Shrimp (Penaeus vannamei) in Mice via Enhanced Intestinal Barrier Function and Antigen Presentation Suppression","authors":"Qiaozhen Liu, Songyi Lin, Kexin Liu, Shuqi Jia, Shuya Wang, Na Sun","doi":"10.1021/acs.jafc.4c09800","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09800","url":null,"abstract":"Covalent modification is an effective strategy for reducing the allergenicity of single allergens. However, due to the complexity of the food matrix, its application in hypoallergenic food production requires further exploration. The study showed that covalent modification of fucoidan decreased the specific antibody levels, inhibited Th2 cell differentiation, and reduced mast cell degranulation, suggesting that it significantly reduced the allergenicity of <i>Penaeus vannamei</i>. Further analysis showed that covalent modification not only up-regulated the intestinal tight junction proteins expression and improved intestinal mucus secretion but also restored intestinal microbial homeostasis by immunological and 16S rRNA gene sequencing. Additionally, covalent modification inhibited dendritic cell maturation and CD8⁺ T cell differentiation, thereby reducing antigen recognition and presentation, as determined by transcriptome and flow cytometry. Therefore, the covalent modification of fucoidan reduced shrimp allergenicity by enhancing intestinal barrier function and inhibiting antigen presentation. In conclusion, it is a potential strategy for processing hypoallergenic foods.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"44 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gas Chromatography–Tandem Mass Spectrometry Method for Analyses of Iodoform and Diiodomethane in Multiple Biological Matrices from Dairy Cattle","authors":"Marie Rønn, Mette Olaf Nielsen, Natalja P. Nørskov","doi":"10.1021/acs.jafc.4c08419","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08419","url":null,"abstract":"A quantitative method was developed and validated to analyze iodoform and its potential metabolite, diiodomethane, in biological fluids from dairy cows, including rumen fluid, duodenal fluid, blood serum, milk, and urine, using liquid–liquid extraction (LLE) and GC-MS/MS. The method showed no matrix effects across different samples, recoveries of spiked samples between 70 and 120%, and relative standard deviations (RSD%) ranging from 0.7 to 14%. Inter- and intravariations in quality control samples were within 3.1–12.3%, with the highest variation noted in iodoform spiking. Normalized internal standard (IS) and matrix factor (MF) calculations confirmed the suitability of diiodomethane-d2 as an internal standard for quantifying both compounds across different matrices. These results validate the method’s application for ADME (absorption, distribution, metabolism, and excretion) studies, enabling the investigation of iodoform digestion, metabolism, and excretion in dairy cattle.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"52 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin Alleviates Silica-Mediated Lung Fibrosis via PPARγ-PGC-1α Loop and Suppressing Mitochondrial Senescence in Epithelial Cells.","authors":"Weixi Xie, Lang Deng, Xiaohua Zhang, Xiaoting Huang, JinFeng Ding, Wei Liu, Si-Yuan Tang","doi":"10.1021/acs.jafc.4c04887","DOIUrl":"10.1021/acs.jafc.4c04887","url":null,"abstract":"<p><strong>Objective: </strong>Long-term inhalation of silica dust particles leads to lung tissue fibrosis, resulting in impaired gas exchange and increased mortality. Silica inhalation triggers the aging of epithelial cells (AECs), which is a key contributor to the development of pulmonary fibrosis. Myricetin, a flavonoid compound extracted from Myrica genus plants, possesses various biological activities, including antioxidant and immunomodulatory effects. However, the mechanisms underlying myricetin's ability to counter senescence and fibrosis need to be further studied.</p><p><strong>Experimental approach: </strong>In vivo, the antifibrotic and anti-senescence effects of myricetin were evaluated using a silica-induced pulmonary fibrosis mouse model. To further elucidate the mechanisms by which myricetin counteracts silica-induced senescence, in vitro experiments were conducted using AECs.</p><p><strong>Results: </strong>Our studies revealed that myricetin treatment alleviated silica-induced mortality, improved lung function, and reduced the severity of pulmonary fibrosis in mice. Immunofluorescence analysis suggests its potential in mitigating senescence of AECs. Under laboratory conditions, myricetin intervened in the cellular senescence pathway induced by silica dust by modulating mitochondrial function. It acted through the PPARγ-PGC1α axis, effectively reducing silica-induced mitochondrial oxidative stress in AECs, promoting mitophagy, and maintaining mitochondrial dynamics. However, the efficacy of myricetin was reversed under PPARγ siRNA intervention. Additionally, myricetin exhibited an enhancing effect on PPARγ and autophagy in animal models. Treatment with PPARγ and PGC-1α siRNA elucidated the role of myricetin in promoting the formation of a positive feedback loop between PPARγ and PGC-1α. Additionally, the PPARγ inhibitor GW9662 verified the in vivo effects of myricetin.</p><p><strong>Conclusions: </strong>Myricetin activates PPARγ, forming a PPARγ-PGC-1α loop, which promotes mitophagy and maintains mitochondrial dynamics. This alleviates epithelial cell senescence induced by silica exposure, consequently mitigating silica-induced pulmonary fibrosis in mice.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":"27737-27749"},"PeriodicalIF":5.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Allergenicity Analysis of Tropomyosin: A Heat-Stable Allergen in Lateolabrax japonicus","authors":"Meng-Si Li, Fei Xia, Qing-Mei Liu, Jun-Feng Zheng, Tian-Qi Li, Yu-Ni Liao, Gui-Xia Chen, Lian-Zhong Luo, Yi-Xiang Liu, Guang-Ming Liu","doi":"10.1021/acs.jafc.4c10285","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10285","url":null,"abstract":"<i>Lateolabrax japonicus</i>, a prevalent aquatic delicacy, is known to elicit allergic reactions in certain individuals. Nevertheless, the investigation into its allergenic components has remained notably inadequate. In the research, an approximately 35 kDa heat-stable protein of <i>L. japonicus</i> raw/steamed extracts was verified as tropomyosin (TM) by LC–MS/MS. Open reading frame of TM (852 bp) was acquired via PCR amplification, encoding 284 amino acids. The IgE-binding frequency of TM expressed in <i>Escherichia coli</i> was 22.5% among 80 fish-sensitized patients. Furthermore, TM had the ability to activate basophils in 7 patients. In the Balb/c mice model, compared with the PBS group, the levels of specific antibodies (IgE, IgG1, and IgG2a), CD19⁺ B cells, IL-4, and IL-10 were significantly increased in the TM group. However, the opposite was indeed the case for CD4⁺ TCR-β, CD4⁺ CD25⁺ Fox p 3⁺ cells, and IFN-γ. These findings regarding an allergen assist in conducting component-resolved diagnoses and therapeutic research for fish allergy.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"114 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Purification and Characterization of γ-Glutamyl Transpeptidase from Toona sinensis and Its Function on Formation of Sulfur-Containing Volatiles”","authors":"Guanying Shi, Wenyu Gao, Zuobing Xiao, Le Zhang, Pengfei Jiang, Lili Zhao, Zhaogai Wang, Zhimin Xu","doi":"10.1021/acs.jafc.4c11776","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11776","url":null,"abstract":"Zhaogai Wang is now identified as a corresponding author. This article has not yet been cited by other publications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}