Journal of Agricultural and Food Chemistry最新文献_第5页

Dual-Phosphorylated Pumpkin Seed Protein PickeringEmulsions: Structural Evolution, Interfacial Regulation, and CoffeeCreamer Application Quality 双磷酸化南瓜子蛋白榨取乳剂：结构演化、界面调节和咖啡奶精应用质量

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-18 DOI: 10.1021/acs.jafc.6c12935

Jianyu Huang,Dengmi Wang,Ziying Ruan,Hui Xu,Zhizhi Yang,Jicheng Chen

{"title":"Dual-Phosphorylated Pumpkin Seed Protein Pickering\u0000Emulsions: Structural Evolution, Interfacial Regulation, and Coffee\u0000Creamer Application Quality","authors":"Jianyu Huang,Dengmi Wang,Ziying Ruan,Hui Xu,Zhizhi Yang,Jicheng Chen","doi":"10.1021/acs.jafc.6c12935","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c12935","url":null,"abstract":"Abstract Pumpkin seed protein (PSP) was modified with sodium tripolyphosphate (STP) and sodium trimetaphosphate (STMP) to improve Pickering emulsions. Phosphorylation increased with STMP content but plateaued at high ratios. Mass spectrometry identified phosphorylated Ser and Thr residues, while 31P NMR indicated multiple phosphorus environments. Modification altered protein conformation and surface charge, reduced particle size, and improved dispersion without major subunit degradation. Although emulsifying activity decreased, emulsion stability increased; mixed-phosphate treatments generally produced finer droplets and better resistance to centrifugal destabilization than single-phosphate treatments. A 4:1 STP/STMP ratio combined small droplet size with favorable wettability and interfacial adsorption, whereas higher STMP content did not consistently improve overall performance. In coffee beverages, increasing emulsion incorporation intensified phase separation and altered color and flavor. At a 1:9 emulsion-to-coffee ratio, phosphorylated PSP emulsion improved perceived body and smoothness while retaining coffee aroma, outperforming commercial creamer in balance and supporting its potential use as a plant-based food.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, Synthesis, and Herbicidal Activity of Pyrimidinedione-Benzamide Derivatives as Potential Protoporphyrinogen IX Oxidase Inhibitors 吡啶二酮-苯酰胺衍生物作为原卟啉原IX氧化酶抑制剂的设计、合成和除草活性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-18 DOI: 10.1021/acs.jafc.6c11854

Jing-Rui Liu,Yajun Peng,Zhengliang Luo,Chujian Ma,Changhao Yin,Changsheng Ma,Wenyong Jin,Lamei Wu,Dingfeng Luo,Lianyang Bai

{"title":"Design, Synthesis, and Herbicidal Activity of Pyrimidinedione-Benzamide Derivatives as Potential Protoporphyrinogen IX Oxidase Inhibitors","authors":"Jing-Rui Liu,Yajun Peng,Zhengliang Luo,Chujian Ma,Changhao Yin,Changsheng Ma,Wenyong Jin,Lamei Wu,Dingfeng Luo,Lianyang Bai","doi":"10.1021/acs.jafc.6c11854","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11854","url":null,"abstract":"Weeds cause significant yield losses worldwide, and herbicides are essential for their control. Protoporphyrinogen IX oxidase (PPO) is a well-validated herbicide target. In this study, a series of pyrimidinedione benzamide derivatives (A1–A37) were designed, synthesized, and evaluated for herbicidal activity. Most compounds showed excellent activity against dicotyledonous weeds at 37.5 g a.i./ha, and compounds A13 and A20 maintained over 80% inhibition of Amaranthus retroflexus even at 2.34 g a.i./ha. Compounds A7, A13, and A20 displayed broad-spectrum activity against eight weed species, favorable crop safety to wheat and corn, and low toxicity to honey bees. Surface plasmon resonance (SPR) assays confirmed their affinity to Nicotiana tabacum PPO (NtPPO) with KD values of 0.819–2.66 nM, and molecular docking combined with molecular dynamics simulations provided insights into their binding modes. These results suggest that A7, A13, and A20 could be promising PPO inhibitor candidates and provide a useful basis for further optimization.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"42 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Fiber ImprovesHigh-Fat Diet-Induced Hyperglycemiaby Modulating Intestinal ILC3-Mediated Immunity through the SCFA-FFAR2Pathway 膳食纤维通过scfa - ffar2途径改善高脂肪饮食诱导的高血糖，调节肠道ilc3介导的免疫

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-17 DOI: 10.1021/acs.jafc.6c08108

Yi Mou,Feiyang Long,Chenlang Qi,Weisheng Huang,Qilin Du,Yu Zhang,Chang Yu,Jie Zhou

{"title":"Dietary Fiber Improves\u0000High-Fat Diet-Induced Hyperglycemia\u0000by Modulating Intestinal ILC3-Mediated Immunity through the SCFA-FFAR2\u0000Pathway","authors":"Yi Mou,Feiyang Long,Chenlang Qi,Weisheng Huang,Qilin Du,Yu Zhang,Chang Yu,Jie Zhou","doi":"10.1021/acs.jafc.6c08108","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08108","url":null,"abstract":"Abstract Dietary fiber intake is associated with improved glycemic control in high-fat diet-induced hyperglycemia, yet the immunological mechanisms linking gut microbiota-derived metabolites to host defense remain poorly defined. Here, we investigated whether dietary fiber ameliorates these metabolic disorders by modulating intestinal group 3 innate lymphoid cells (ILC3s) via short-chain fatty acids (SCFAs) and their receptor FFAR2. In a 12-week fiber-supplemented high-fat diet mouse model, fiber alleviated hyperglycemia, insulin resistance, and intestinal barrier dysfunction. Mechanistically, fiber increased cecal SCFAs (especially propionate), which activated colonic ILC3s in an FFAR2-dependent manner. FFAR2 triggered ERK and STAT3 phosphorylation and directly bound STAT3 to facilitate its phosphorylation, boosting IL-22 transcription. SCFA-activated ILC3s strengthened epithelial barrier integrity via elevated tight-junction proteins. Collectively, our findings demonstrate that dietary fiber restores gut barrier function through the SCFA-FFAR2-ERK/STAT3-IL-22 axis in ILC 3s, highlighting ILC3-expressed FFAR2 as a potential therapeutic target for high-fat diet-related metabolic disorders.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uptake, Translocation, Subcellular Distribution, andMetabolism of Fluoxapiprolin in Cucumber Plants 氟沙匹prolin在黄瓜植物中的吸收、转运、亚细胞分布和代谢

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-17 DOI: 10.1021/acs.jafc.6c08513

Yanli Bian,Na Wang,Xiupeng Yang,Kaikai Zhu,Jinju Pan,Yizhi Feng

{"title":"Uptake, Translocation, Subcellular Distribution, and\u0000Metabolism of Fluoxapiprolin in Cucumber Plants","authors":"Yanli Bian,Na Wang,Xiupeng Yang,Kaikai Zhu,Jinju Pan,Yizhi Feng","doi":"10.1021/acs.jafc.6c08513","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08513","url":null,"abstract":"Abstract Fluoxapiprolin is an oxysterol-binding protein fungicide that exhibits high efficacy against cucumber downy mildew; however, its metabolites may be more toxic than the parent compound. In this study, we examined its uptake, translocation, subcellular distribution, and metabolism in cucumbers. Fluoxapiprolin primarily accumulated in roots through aquaporin-mediated passive absorption and apoplastic transport and showed poor upward translocation. The behavior of the five metabolites varied. BCS-DE72761 and BCS-DE61185 accumulated continuously in leaves, whereas BCS-BP32808 exhibited the strongest upward translocation. In roots and stems, 88–100% of the metabolites were distributed in soluble components. Fluoxapiprolin was primarily transformed via amide bond cleavage into BCS-CC26101, the reversible interconversion of BCS-DE61185 and BCS-DE72761 through amidation and hydrolysis, as well as the transformation of BCS-BP32808 into multiple metabolites. These reactions can alter the physicochemical properties of the compounds, thereby significantly affecting their translocation in plants. Our findings provide a theoretical basis for scientific application and risk assessment of these compounds.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"167 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retention-GuidedLC-MS/MS Enables Discovery and StructuralAssignment of Trace Steviol Glycosides from in Vitro Glycosylation Reactions 保留导向的lc -MS/MS能够从体外糖基化反应中发现痕量甜菊醇糖苷并进行结构分配

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-17 DOI: 10.1021/acs.jafc.6c04961

Takehiro Watanabe,Kohki Fujikawa,Kazunari Iwaki,Tadayoshi Hirai,Jun Murata

{"title":"Retention-Guided\u0000LC-MS/MS Enables Discovery and Structural\u0000Assignment of Trace Steviol Glycosides from\u0000 in Vitro\u0000 Glycosylation Reactions","authors":"Takehiro Watanabe,Kohki Fujikawa,Kazunari Iwaki,Tadayoshi Hirai,Jun Murata","doi":"10.1021/acs.jafc.6c04961","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c04961","url":null,"abstract":"Abstract Stevia rebaudiana produces diverse steviol glycosides (SVGs), but many positional isomers remain difficult to distinguish by MS/MS alone because of similar fragmentation patterns and low abundance. Here, we established a retention-guided LC–MS/MS workflow for discovery and structural characterization of trace SVGs generated by in vitro glycosylation using crude enzyme extracts from Stevia leaves. Reversed-phase retention trends associated with sugar identity and linkage patterns enabled chromatographic discrimination of isomeric SVGs, including rebaudiosides D and I, which exhibit indistinguishable MS/MS and MS3 spectra. Enzymatic reactions using UDP–13C6-glucose and UDP-xylose generated rare sugar-chain elongated derivatives, including rebaudioside FX1 and the previously unreported compound Reb FX0 carrying Glcβ(1–2)Glcβ1– at C-13 and Xylβ(1–2)Glcβ1– at C-19. Structural assignments were confirmed by comparison with semisynthetic standards. This integrated analytical–biochemical approach provides a practical platform for discovery-oriented characterization of trace SVGs and supports compositional analysis of stevia-derived ingredients and bioconversion products relevant to food applications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated MetabolomicsReveals Chemical Differentiationamong Andean Rubus Species and R. glaucus Genotypes 综合代谢组学揭示安第斯红莓种和青花紫莓基因型之间的化学差异

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-17 DOI: 10.1021/acs.jafc.6c09379

Nathalia Cardona-Hurtado,Gloria Edith Guerrero-Álvarez,Ana M. León-Inga,Ana María López,Mónica P. Cala

{"title":"Integrated Metabolomics\u0000Reveals Chemical Differentiation\u0000among Andean\u0000 Rubus\u0000 Species and\u0000 R. glaucus\u0000 Genotypes","authors":"Nathalia Cardona-Hurtado,Gloria Edith Guerrero-Álvarez,Ana M. León-Inga,Ana María López,Mónica P. Cala","doi":"10.1021/acs.jafc.6c09379","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09379","url":null,"abstract":"Abstract The metabolic diversity of Rubus fruits is relevant for authentication, nutritional assessment, and breeding, but biochemical information about Andean germplasm remains limited. This study aimed to characterize phytochemical variation at interspecific and intraspecific levels by integrating untargeted LC–QTOF-MS, targeted LC–QqQ-MS phenolic profiling, antioxidant assays, and molecular networking. Three Rubus materials and five Rubus glaucus genotypes were evaluated. Chemometric analysis revealed metabolic separation among the Rubus materials, with 63 differentially annotated metabolites in the untargeted analysis and 25 compounds significantly different in targeted profiling. Among R. glaucus genotypes, 79 differentially annotated metabolites and 12 targeted compounds showed differences. Discriminatory metabolites included phenylpropanoid-derived phenolics, organic acids, and glycerophospholipids. R. glaucus materials showed higher antioxidant capacity in several assays, with differences in phenolic composition. Correlation analysis revealed associations among phenolic, primary carbon, and lipid-related metabolism. The integrated approach characterized metabolic variation and identified metabolites associated with their chemical differentiation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"33 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

13C{1H} NMR-Guided Identificationof a New Marker of Caramel Color IV 13C{1H}核磁共振引导鉴定一种新的焦糖颜色标记物

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c12495

Xixuan Zhao,Boan Yao,Shenghao Che,Qian Zhang,Pengying He,Longchen Zhang,Yongguo Liu,Baoguo Sun,Hongyu Tian,Sen Liang

引用次数: 0

Tea PolyphenolEGCG Prevents UVB-Induced OxidativeStress via SIRT3-Mediated Activation of SOD2: Mechanistic Insightsfrom Molecular Interactions 茶多酚egcg通过sirt3介导的SOD2激活来阻止uvb诱导的氧化应激：来自分子相互作用的机制见解

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c04934

Titi Liu,Siwei Xiong,Jing Xu,Yingqi Yin,Ziyan Yang,Hengrui Liang,Jia Liu,Changqun Li,Xianhui Xiang,Mingxing Che,Dengyou Nie,Lei Peng,Jun Sheng,Chaoyi Xue,Ya Wang,Huanhuan Xu

{"title":"Tea Polyphenol\u0000EGCG Prevents UVB-Induced Oxidative\u0000Stress via SIRT3-Mediated Activation of SOD2: Mechanistic Insights\u0000from Molecular Interactions","authors":"Titi Liu,Siwei Xiong,Jing Xu,Yingqi Yin,Ziyan Yang,Hengrui Liang,Jia Liu,Changqun Li,Xianhui Xiang,Mingxing Che,Dengyou Nie,Lei Peng,Jun Sheng,Chaoyi Xue,Ya Wang,Huanhuan Xu","doi":"10.1021/acs.jafc.6c04934","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c04934","url":null,"abstract":"Abstract UVB irradiation triggers skin photodamage via oxidative stress and mitochondrial dysfunction. This study investigated the photoprotective mechanism of EGCG, the most abundant polyphenol in green tea, in HaCaT keratinocytes. EGCG pretreatment attenuated UVB-induced viability loss and oxidative stress, evidenced by reduced 4-hydroxy-2-nonenal, protein carbonyls, and ROS, alongside restored glutathione redox balance. EGCG recovered SIRT3 enzymatic activity without affecting its expression and promoted SOD2 deacetylation at Lys68 and Lys122 in vitro. SPR and ITC confirmed direct, high-affinity binding of EGCG to SIRT3, with molecular dynamics simulations revealing a stable complex. EGCG also enhanced the binding affinity between SIRT3 and its acetylated p53 substrate in vitro. Critically, pharmacological inhibition of SIRT3 by 3-TYP abolished EGCG’s protective effects, supporting a critical role of SIRT3 in EGCG-mediated protection. Collectively, these findings indicate that EGCG protects against UVB-induced skin photodamage by binding to and activating SIRT3, thereby promoting SIRT3-mediated SOD2 deacetylation to alleviate oxidative stress.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-Responsive HydrogelsEncapsulating SolubilizedEllagic Acid Enhance Microbial Conversion to Urolithin A 包封可溶解鞣花酸的ph响应水凝胶促进微生物向尿素A的转化

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c07197

Haozhen Zhang,Liye Hu,Ruiqi Qian,Jike Shuai,Haibo Pan,Donghong Liu,Xingqian Ye,Shiguo Chen

{"title":"pH-Responsive Hydrogels\u0000Encapsulating Solubilized\u0000Ellagic Acid Enhance Microbial Conversion to Urolithin A","authors":"Haozhen Zhang,Liye Hu,Ruiqi Qian,Jike Shuai,Haibo Pan,Donghong Liu,Xingqian Ye,Shiguo Chen","doi":"10.1021/acs.jafc.6c07197","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c07197","url":null,"abstract":"Abstract Microbial conversion of poorly soluble ellagic acid (EA) to urolithin A (UroA) is limited by poor aqueous performance and gastrointestinal presentation. Here, we combined pH-shift-assisted sono-assembly with CaCO3/GDL-mediated internal gelation to integrate EA physicochemical-state modification with gastrointestinal delivery. Solubilized EA (sEA) showed improved aqueous retention and equilibrium solubility, while pH-responsive alginate encapsulation restricted gastric release to below 15% and enhanced EA preservation during digestion. The 1.5% alginate internal-gelation hydrogel encapsulating sEA (IG1.5%@sEA) retained 88.21% of EA after digestion and produced the highest UroA concentration (36.86 μg/mL at 48 h) with urolithin metabotype A microbiota. Comparisons with sEA and IG1.5%@EA, together with fermentation at the same postdigestion EA concentrations, showed that both gastrointestinal preservation and the physicochemical state of EA after solubilization contributed to enhanced microbial conversion. These findings support delivery systems coordinating gastrointestinal protection with control of substrate physicochemical state to enhance microbial conversion of poorly soluble polyphenols.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"24 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characteristicsof the Novel ProteinaceousInhibitor against Pancreatic α-Amylase from White KidneyBean ( Phaseolus vulgaris ) 白菜豆胰α-淀粉酶蛋白抑制剂的分离及特性研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-10-16 DOI: 10.1021/acs.jafc.6c12164

Yiting Wang,Jingyi Lu,Jialei Chu,Guangrong Huang,Wenwei Chen,Zhenbao Jia,Fei Tao

{"title":"Isolation and Characteristics\u0000of the Novel Proteinaceous\u0000Inhibitor against Pancreatic α-Amylase from White Kidney\u0000Bean (\u0000 Phaseolus vulgaris\u0000 )","authors":"Yiting Wang,Jingyi Lu,Jialei Chu,Guangrong Huang,Wenwei Chen,Zhenbao Jia,Fei Tao","doi":"10.1021/acs.jafc.6c12164","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c12164","url":null,"abstract":"Abstract A novel proteinaceous α-amylase inhibitor was isolated from white kidney bean (Phaseolus vulgaris) by ammonium sulfate fractionation followed by DEAE-Sepharose Fast Flow and HiTrap DEAE-Sepharose Fast Flow chromatography. The purified inhibitor was identified as annexin (UniProt accession: V7BSM8) by HPLC-MS/MS, with a molecular mass of 35.97 kDa determined by MALDI-TOF MS. Annexin inhibited pancreatic α-amylase in a concentration-dependent manner, with an IC50 of 8.81 μM. The kinetic results were consistent with a noncompetitive inhibition pattern. Intrinsic fluorescence spectroscopy indicated that annexin binding to α-amylase induced static fluorescence quenching and conformational changes in the enzyme. Molecular dynamics simulations supported formation of a stable α-amylase–annexin complex, with electrostatic and van der Waals interactions as major energetic contributors. These findings identify annexin as a previously unrecognized α-amylase inhibitor from P. vulgaris with potential for modulating starch digestion and postprandial glycemic responses.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149209809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0