Journal of Agricultural and Food Chemistry最新文献_第4页

Dimeric Amide Alkaloid Enantiomers from Piper longum L. with Anti-Inflammatory and Antidiabetic Effects

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-11 DOI: 10.1021/acs.jafc.4c13133

Guanghui Gou, Liu Liu, Wenli Bao, Jun Li, Haji Akber Aisa

{"title":"Dimeric Amide Alkaloid Enantiomers from Piper longum L. with Anti-Inflammatory and Antidiabetic Effects","authors":"Guanghui Gou, Liu Liu, Wenli Bao, Jun Li, Haji Akber Aisa","doi":"10.1021/acs.jafc.4c13133","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c13133","url":null,"abstract":"The fruit of Piper longum L. (Piperaceae), commonly used as a spice in various culinary applications, is renowned for its rich nutritional profile and potential medicinal properties. In this study, a molecular network-based dereplication strategy was employed to isolate 12 dimeric amide alkaloid enantiomers (1–12) from P. longum fruits, including eight pairs of cyclobutane-type dimers (1–8) and four pairs of cyclohexene-type dimers (9–12). Notably, five pairs of cyclobutane-type dimers, namely, piperlongramides E–I (1–5), and one pair of cyclohexene-type dimers, piperlongramide J (9), were identified as undescribed compounds. The structures of these compounds were elucidated by comprehensive spectroscopic data, electronic circular dichroism (ECD) calculations, and X-ray diffraction analysis. Additionally, these compounds, for the first time, were subjected to chiral resolution. In vitro bioactivity screening revealed that compounds (+)-10, (−)-10, and (+)-3 exhibited notable anti-inflammatory effects in an LPS-induced RAW 264.7 macrophage model, with IC50 values of 23.42 ± 1.04, 32.72 ± 0.54, and 33.52 ± 1.75 μM, respectively. Furthermore, compound (+)-3 also demonstrated significant inhibitory activity againstα-glucosidase, with an IC50 of 11.69 ± 0.91 μM. Compounds (+)-9, (+)-7, and (−)-9, exhibited promising inhibitory activity against PTP1B with IC50 values of 3.30 ± 0.09, 4.30 ± 0.18, and 4.37 ± 0.37 μM, respectively, indicating their potential antidiabetic effects. This study highlights the promising application of these amide alkaloid dimers in the development of functional foods and pharmaceutical products, thereby expanding the health-promoting potential of P. longum fruits.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"86 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of a Fluxapyroxad Nanoformulation with Strong Plant Uptake for Efficient Control of Verticillium Wilt in Potato

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-11 DOI: 10.1021/acs.jafc.4c12450

Dong Wang, Huiting Weng, Yuanzheng Zhao, Hongyou Zhou, Huiming Guo, Hongmei Cheng, Jie Shen, Meizhen Yin, Shuo Yan, Xiaofeng Su

{"title":"Preparation of a Fluxapyroxad Nanoformulation with Strong Plant Uptake for Efficient Control of Verticillium Wilt in Potato","authors":"Dong Wang, Huiting Weng, Yuanzheng Zhao, Hongyou Zhou, Huiming Guo, Hongmei Cheng, Jie Shen, Meizhen Yin, Shuo Yan, Xiaofeng Su","doi":"10.1021/acs.jafc.4c12450","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12450","url":null,"abstract":"Potato (Solanum tuberosum L.) is ranked as the fourth largest staple crop in China. However, potato production is increasingly threatened by Verticillium wilt (VW) caused by the fungus Verticillium dahliae in various provinces. In the present study, we explored the application of star polycation (SPc) nanocarrier to improve the effectiveness of the fungicide fluxapyroxad (Flu) in combating VW. The SPc self-assembled with Flu through hydrogen bonds and van der Waals forces to form the Flu/SPc complex spontaneously, which exhibited strong intermolecular interactions, as indicated by a high affinity constant and favorable thermodynamic parameters. Complexation with SPc decreased the particle size of Flu. The Flu/SPc complex had a greater effect on V. dahliae than Flu alone, reducing the colony diameter and spore numbers more effectively. Expression levels of multiple key genes involved in nitrogen, polysaccharide, and sugar metabolism were downregulated in V. dahliae upon Flu/SPc complex treatment compared to Flu treatment, which might contribute to the greater growth inhibition in Flu/SPc-treated samples. Uptake studies in potato plants demonstrated that SPc significantly enhanced the absorption of Flu compared with Flu alone. Slighter disease symptoms and lower fungal biomass in greenhouse and field trials confirmed the enhanced protective effects of the Flu/SPc complex on potato seedlings. This is the first report that a self-assembled nanofungicide limits V. dahliae growth and protects potatoes from destructive VW.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"16 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactiplantibacillus plantarum FEED8 Biosynthesis of Pyranoanthocyanin (Cyanidin-3-glucoside-4-vinylcatechol) Improves Oxidative Stress and Inflammation of the Gut Microbiome in Cadmium-Exposed Mice

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-11 DOI: 10.1021/acs.jafc.5c01093

Qing Zhang, Yue Li, Yudi Han, Weijie Zhou, Xusheng Li, Jianxia Sun, Weibin Bai

{"title":"Lactiplantibacillus plantarum FEED8 Biosynthesis of Pyranoanthocyanin (Cyanidin-3-glucoside-4-vinylcatechol) Improves Oxidative Stress and Inflammation of the Gut Microbiome in Cadmium-Exposed Mice","authors":"Qing Zhang, Yue Li, Yudi Han, Weijie Zhou, Xusheng Li, Jianxia Sun, Weibin Bai","doi":"10.1021/acs.jafc.5c01093","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01093","url":null,"abstract":"The study is to explore the biosynthesis of cyanidin-3-glucoside-4-vinylcatechol (C3G_VC) through Lactiplantibacillus plantarum-fermented caffeic acid and cyanidin-3-glucoside (C3G) extract (molar ratio = 1:30) in the model medium. C3G_VC was isolated and purified by a venusil ASB-C18 column with a medium-pressure liquid chromatography (MPLC) system. The chemical structure of C3G_VC was identified by high-performance liquid chromatography (HPLC), which showed the maximum absorption wavelength of 505.57 nm. This study showed that Cd exposure of mice induced liver damage, oxidative stress, and inflammation of the gut microbiome. Our findings demonstrated that C3G_VC intervention in Cd-exposed mice significantly mitigated oxidative stress injury by declining the malondialdehyde (MDA) level and increasing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in the liver, meanwhile alleviating liver injury by decreasing the bile acid (BA) level and accelerating the excretion of fecal BA. Moreover, the Cd_C3G_VC group showed elevated levels of mRNA expression of pro-inflammatory cytokines (IL6, IL1β, and TNF-α) and inhibited BA synthesis (CYP7A1) in Cd-exposed mice. The fermentation results in vitro showed that C3G_VC had a higher residue than that of cyanidin-3-glucoside. The 16S rRNA high-throughput sequencing disclosed that C3G_VC intervention in Cd-exposed mice significantly increased the abundance of Faecalibaculum and unidentified_Lachnospiraceae. It is noteworthy that the C3G_VC supplement increased the abundance of Akkermansia. Overall, this study demonstrated that C3G_VC intervention in Cd-exposed mice had the potential to decrease the occurrence of inflammatory and oxidative stress and maintain bile acid homeostasis by regulating gut microflora.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"20 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Myrosinase-Mediated Glucosinolate Degradation Pathways in Lactiplantibacillus plantarum ZUST49

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.4c11728

Xili Liang, Caoyuan Niu, Chunmin Jiang, Dajing Li, Ligen Zou, Yao Zhang, María José Benito, Yuheng Cheng, Yuanfeng Wu

{"title":"Characterization of Myrosinase-Mediated Glucosinolate Degradation Pathways in Lactiplantibacillus plantarum ZUST49","authors":"Xili Liang, Caoyuan Niu, Chunmin Jiang, Dajing Li, Ligen Zou, Yao Zhang, María José Benito, Yuheng Cheng, Yuanfeng Wu","doi":"10.1021/acs.jafc.4c11728","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11728","url":null,"abstract":"Cruciferous vegetables are rich in glucosinolates that can be hydrolyzed by myrosinase into isothiocyanates (ITCs) with significant anticancer properties. In the absence of bacterial myrosinase, glucosinolates are excreted from the body in their inactive forms. However, the mechanisms underlying the bacterial breakdown of glucosinolates are not well understood. Here, we investigated the mechanism and enzymes involved in glucosinolate breakdown by the probiotic microorganism Lactiplantibacillus plantarum ZUST49, which degrades the glucosinolate glucoraphanin to sulforaphane and erucin. The glucoraphanin-degrading activity of this strain was induced by the presence of glucoraphanin and an absence of glucose. UPLC-MS analysis of the degradation products indicated that glucoraphanin was degraded via three distinct pathways, and further, transcriptomic and proteomic analyses led to the identification of a myrosinase gene, LpMyr, that encodes a 460-amino acid enzyme. The purified LpMyr protein exhibited optimal activity at 50 °C and pH 7.0, with hydrolysis rates of 7.74 U/mg for glucoraphanin and 5.89 U/mg for sinigrin. These findings provide new insights into the glucosinolate conversion capability of L. plantarum and highlight its potential for high-yield ITC production in the fermentation industry, as well as its potential use as a probiotic in the human gut.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function Identification of a Lactonase Gene lac1563 Involved in Producing Urolithin A of Limosilactobacillus fermentum FUA033

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.4c10908

Ze Wang, Xiaoli Liu, Shu Liu, Wenmei Zhou, Tao Chen, Song Gao, Hao Wu, Wenjun Zhang, Rui Tang, Yaowei Fang

{"title":"Function Identification of a Lactonase Gene lac1563 Involved in Producing Urolithin A of Limosilactobacillus fermentum FUA033","authors":"Ze Wang, Xiaoli Liu, Shu Liu, Wenmei Zhou, Tao Chen, Song Gao, Hao Wu, Wenjun Zhang, Rui Tang, Yaowei Fang","doi":"10.1021/acs.jafc.4c10908","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10908","url":null,"abstract":"This study aimed to identify the lactonase gene in Limosilactobacillus fermentum FUA033 that facilitates the conversion of ellagic acid into urolithin A. Lactonase gene candidates were identified genome analysis. A series of overexpression, knockout, and complementation strains of these candidate genes were subsequently generated. The urolithin A yield of the lac1563 knockout strain was significantly reduced compared to the wild-type strain, whereas the overexpression strain exhibited a markedly higher yield. The complementation strain showed no significant difference in urolithin A yield relative to the wild-type strain. In contrast, alterations in the lac329 gene did not significantly impact urolithin A production compared to the wild-type strain. Additionally, the candidate lactonase genes were cloned and heterologously expressed in Escherichia coli. The recombinant lactonase derived from the lac1563 gene, unlike that from the lac329 gene, demonstrated activity toward ellagic acid. Optimal activity of the lac1563 lactonase occurred at 37 °C and pH 7.0. Enzyme activity was notably enhanced by Mn2+ and Co2+, while inhibited by Zn2+ and Cu2+. These findings contribute valuable insights into the metabolic pathway of ellagic acid conversion to urolithin A and establish the lac1563 gene as a promising candidate for biotechnological applications aimed at urolithin A production.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"8 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binding Properties of Chemosensory Protein 1 to Plant Volatiles Guiding Nicotiana rustica Recognition in Helicoverpa armigera.

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.5c01664

Ya-Lan Sun, Ya-Hui Sun, Ke Wang, Pengjun Xu, Jun-Feng Dong

{"title":"Binding Properties of Chemosensory Protein 1 to Plant Volatiles Guiding Nicotiana rustica Recognition in Helicoverpa armigera.","authors":"Ya-Lan Sun, Ya-Hui Sun, Ke Wang, Pengjun Xu, Jun-Feng Dong","doi":"10.1021/acs.jafc.5c01664","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01664","url":null,"abstract":"Helicoverpa armigera is one of the most serious pests attacking tobacco around the world. Nicotiana rustica is widely used as a trap crop in the fields of Nicotiana tabacum in China. However, the chemical and molecular bases guiding N. rustica recognition in H. armigera are still unknown. In this study, we found 15 volatiles emitted from N. rustica induced EAG responses of H. armigera, while β-ocimene, α-pinene, and linalool were attractive to the moths in Y-tubes. Oviposition tests showed that H. armigera prefers to lay eggs on tobacco plants treated with β-ocimene and α-pinene. Further assays showed that an antenna-enriched chemosensory protein, HarmCSP1, binds robustly with β-ocimene, benzaldehyde, and (Z)-3-hexenyl acetate. Structural analyses revealed that several key amino acid residues are involved in ligand binding. This study increases the understanding of the chemosensory mechanism of H. armigera's recognition of N. rustica and provides the foundation for the design of attractants for H. armigera management.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Mining of Epicoccum dendrobii Reveals Diverse Antimicrobial Natural Products

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.4c11808

Anxin Zhang, Xinran Xu, Wen-Bing Yin

{"title":"Genome Mining of Epicoccum dendrobii Reveals Diverse Antimicrobial Natural Products","authors":"Anxin Zhang, Xinran Xu, Wen-Bing Yin","doi":"10.1021/acs.jafc.4c11808","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11808","url":null,"abstract":"Epicoccum dendrobii, a biocontrol fungus, produces bioactive metabolites with potential agricultural applications. Targeted genome mining revealed 13 compounds, including three novel polyketides (epiketides A–C, 1–3), a new natural product (epiketide D, 4), a diketopiperazine (boydine E, 5), and eight known analogues 6–13. Structures were elucidated via NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and computational methods. Compounds 1, 2 and 4 exhibited moderate antibacterial activity against Bacillus licheniformis (minimum inhibitory concentrations (MICs): 5.5–37.6 μM) and Pseudomonas aeruginosa (MICs: 11.1–20.0 μM). A protoplast transformation system was developed to identify the biosynthetic gene cluster eda responsible for diketopiperazines 5–10. Antifungal assays revealed that wild-type E. dendrobii inhibited plant pathogen Botrytis cinerea by 56.03%, while the ΔedaP mutant (lacking diketopiperazines) showed reduced efficacy, implying the role of diketopiperazines in antifungal activity. This study underscores E. dendrobii’s potential as a biocontrol agent and provides insights into its bioactive metabolites for agricultural applications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"28 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Differential Metabolite Active Ingredients in Maize Roots Based on Network Pharmacology and Metabolomics Analysis.

IF 5.7 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.4c08012

Xiaotong Wei, Peng Jiao, Zhenzhong Jiang, Chunlai Wang, Qiang Liu, Yuexuan Li, Siyan Liu, Shuyan Guan, Yiyong Ma

{"title":"Study on Differential Metabolite Active Ingredients in Maize Roots Based on Network Pharmacology and Metabolomics Analysis.","authors":"Xiaotong Wei, Peng Jiao, Zhenzhong Jiang, Chunlai Wang, Qiang Liu, Yuexuan Li, Siyan Liu, Shuyan Guan, Yiyong Ma","doi":"10.1021/acs.jafc.4c08012","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08012","url":null,"abstract":"Maize is a globally important crop. Roots are the main part of maize and are mainly used for soil improvement and for maintaining crop growth as agricultural waste. Their application scope is relatively small. It is very important to analyze the components in maize roots in order to increase their resource utilization and reduce the burden of waste disposal. Metabolomics shows that maize roots contain various bioactive components, such as alkaloids, phenolic acids, flavonoids, etc. Therefore, this study explores the potential pharmacological effects of maize root metabolites under drought stress from the perspective of metabolomics combined with network pharmacology. The crude extraction of betaine, a metabolite in maize roots under drought stress, was conducted, and the anti-inflammatory and antioxidant effects of the crude extract were evaluated. The experimental results of DPPH, Fenton, etc. indicate that the crude extract of betaine from maize roots has certain anti-inflammatory and antioxidant effects, which provides a basis for its potential applications in the fields of medicine and food. The research on extracting medicinal active substances such as betaine from maize roots as agricultural waste not only has economic and environmental advantages but also has important significance in promoting technological progress and public health.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Compositions and Antibacterial Activities of Litsea cubeba Essential Oil and Its Distillates Prepared by Vacuum Fractional Distillation and Molecular Distillation

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.4c11955

Qingqing Chen, Xiaocai Lin, Jia Zhang, Shichen Liu, Ziqing Zou, Jiong Chun

{"title":"Chemical Compositions and Antibacterial Activities of Litsea cubeba Essential Oil and Its Distillates Prepared by Vacuum Fractional Distillation and Molecular Distillation","authors":"Qingqing Chen, Xiaocai Lin, Jia Zhang, Shichen Liu, Ziqing Zou, Jiong Chun","doi":"10.1021/acs.jafc.4c11955","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11955","url":null,"abstract":"Litsea cubeba essential oil (LCEO) has various bioactivities and wide applications. However, most reported LCEOs are directly extracted from plants, and studies on further processing of LCEO to enrich bioactive components using modern separation techniques are scarce. In this study, LCEO was extracted by hydrodistillation and further processed via vacuum fractional distillation (VFD) and molecular distillation (MD). The chemical compositions of LCEO and seven distillates were analyzed, and the activities of the EOs and eight individual constituents against seven bacteria were tested. Distillates VFD3 and MDH2 showed the best activity against Escherichia coli, Staphylococcus aureus, and Agrobacterium rhizogenes. VFD3 exerted antibacterial action against A. rhizogenes by inhibiting biofilms, damaging the cell membrane and cell wall, and perturbing metabolic pathways. VFD and MD are effective processing methods for changing the chemical composition and enhancing the bioactivity of LCEO, which might be used to improve the quality and extend the applications of LCEO and other EOs.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"16 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitivity to Boscalid and Trifloxystrobin and Fitness of Corynespora cassiicola Associated with CcSdh & CcCytb in Cucumber

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI: 10.1021/acs.jafc.4c12431

Guangxue Zhu, Yuanyuan Yue, Rongjia Zhou, Bingxue Sun, Xuewen Xie, Ali Chai, Lei Li, Tengfei Fan, Baoju Li, Yanxia Shi

{"title":"Sensitivity to Boscalid and Trifloxystrobin and Fitness of Corynespora cassiicola Associated with CcSdh & CcCytb in Cucumber","authors":"Guangxue Zhu, Yuanyuan Yue, Rongjia Zhou, Bingxue Sun, Xuewen Xie, Ali Chai, Lei Li, Tengfei Fan, Baoju Li, Yanxia Shi","doi":"10.1021/acs.jafc.4c12431","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12431","url":null,"abstract":"The extensive use of succinate dehydrogenase inhibitors (SDHIs) and quinone outside inhibitors (QoIs) has led to the development of resistance in Corynespora cassiicola, which causes corynespora leaf spot (CLS) in cucumber. In this study, the sensitivity and the resistance phenotypes to SDHIs and QoIs were monitored, and the fitness of C. cassiicola carrying CcSdh & CcCytb mutations was measured. The average 50% effective suppressive concentration (EC50) values of C. cassiicola to boscalid from 2019 to 2021 ranged from 6.65 to 24.16 μg/mL, while the average EC50 values of C. cassiicola to trifloxystrobin from 2007 to 2021 ranged from 105.11 to 462.02 μg/mL. Ten fungicide resistance phenotypes and 19 resistance genotypes were found. The D-D95E&Cytb-G143A and B–H278L&Cytb-G143A mutants presented increased fitness, while the B–H278Y&Cytb-G143A mutant showed significant fitness costs, and the other mutants presented no or slight fitness costs. These findings provide a basis for the formulation of resistance management strategies of CLS.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0