Journal of Agricultural and Food Chemistry最新文献_第3页

In Vitro Infant Fecal Fermentation Metabolites of Osteopontin and 2′-Fucosyllactose Support Intestinal Barrier Function 婴儿粪便体外发酵代谢物骨化蛋白和 2′-岩藻糖支持肠道屏障功能

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-20 DOI: 10.1021/acs.jafc.4c07683

Ran Cai, Yuxing Zheng, Jonathan A. Lane, Pantian Huang, Ruibiao Hu, Qiang Huang, Feitong Liu, Bin Zhang

{"title":"In Vitro Infant Fecal Fermentation Metabolites of Osteopontin and 2′-Fucosyllactose Support Intestinal Barrier Function","authors":"Ran Cai, Yuxing Zheng, Jonathan A. Lane, Pantian Huang, Ruibiao Hu, Qiang Huang, Feitong Liu, Bin Zhang","doi":"10.1021/acs.jafc.4c07683","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07683","url":null,"abstract":"In this study, we investigated the effects of infant fecal fermentation-derived metabolites of digested osteopontin (OPN) and 2′-fucosyllactose (2′-FL), either individually or in combination, on intestinal barrier function using a Caco-2/HT-29 coculture cell model. Our results suggested that the OPN/2′-FL (1:36–1:3) cofermentation metabolites improved epithelial barrier integrity by supporting the mRNA and protein expression of occludin, claudin-1, claudin-2, ZO-1, and ZO-2. All of the OPN/2′-FL treatments decreased the production of IL-1β, IL-6, and TNF-α, while the OPN/2′-FL ratio increased IL-10 production by inhibiting activation of the MyD88/IκB-α/NF-κB signaling pathway. OPN/2′-FL cofermentation altered the metabolic pathways, and the protective effect of fermentation metabolites on intestinal barrier function was related to differential metabolite expression such as short-chain fatty acids, deoxycholic acid, and 4-aminobutyric acid. Our findings provide in vitro evidence to support the application of the OPN/2′-FL combination in infant formula for the advancement of formulation functionality, including intestinal barrier function.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"111 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal–Organic Framework-Based Cascade Catalysis-Enabled Fluorescent ELISA with Higher Enzymatic Stability for Zearalenone Detection in Maize

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-20 DOI: 10.1021/acs.jafc.4c07146

Chaochao Chen, Liang Luo, Jianzhong Shen, Zhanhui Wang, Yantong Pan

{"title":"Metal–Organic Framework-Based Cascade Catalysis-Enabled Fluorescent ELISA with Higher Enzymatic Stability for Zearalenone Detection in Maize","authors":"Chaochao Chen, Liang Luo, Jianzhong Shen, Zhanhui Wang, Yantong Pan","doi":"10.1021/acs.jafc.4c07146","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07146","url":null,"abstract":"The enzyme-linked immunosorbent assay (ELISA) stands as one of the most frequently employed rapid detection techniques for both chemical and biological contaminants. Horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), serving as signal generators, play indispensable roles throughout the entire ELISA process. However, HRP and H2O2 exhibit high sensitivity to elevated temperatures, hindering the broader utilization and transportation of ELISA. Hereby, being inspired by the cascade catalysis of glucose by glucose oxidase (GOx) and HRP as well as the advantageous properties of metal–organic frameworks (MOFs), we developed a cascade catalysis-based competitive fluorescent ELISA (CCF-ELISA) for mycotoxin detection in maize using zearalenone (ZEN) as a model. The results revealed the remarkable protective effects of MOFs on enzymatic activities. Based on upon characteristics, the built CCF-ELISA enabled the detection of ZEN with a half-maximal inhibitory concentration of 0.54 ng/mL and allowed a detection range from 0.19 to 1.51 ng/mL. When applied to naturally contaminated maize samples, the outcomes obtained from the CCF-ELISA showed a high level of comparability with high-performance liquid chromatography–tandem mass spectrometry, demonstrating the promising potential of the developed CCF-ELISA in the rapid detection for food security.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"82 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Lactose-Derived α-Dicarbonyl Compounds in Dairy Products and Elucidation of Their Formation Mechanism

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-20 DOI: 10.1021/acs.jafc.4c08966

Yuri Nomi, Takuma Anazawa, Kazumi Shinzawa, Moeka Tamura, Hitoshi Matsumoto

引用次数: 0

Multiple Strategies Enhance 7-Dehydrocholesterol Production from Kitchen Waste by Engineered Yarrowia lipolytica

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c09552

Tianyu Dong, Xiao Zhou, Zheng-Jie Hou, Yujie Shu, Mingdong Yao, Zhi-Hua Liu, Jing-Sheng Cheng, Wenhai Xiao, Ying Wang

{"title":"Multiple Strategies Enhance 7-Dehydrocholesterol Production from Kitchen Waste by Engineered Yarrowia lipolytica","authors":"Tianyu Dong, Xiao Zhou, Zheng-Jie Hou, Yujie Shu, Mingdong Yao, Zhi-Hua Liu, Jing-Sheng Cheng, Wenhai Xiao, Ying Wang","doi":"10.1021/acs.jafc.4c09552","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09552","url":null,"abstract":"7-Dehydrocholesterol (7-DHC) is an important precursor of vitamin D3. The microbial synthesis of 7-DHC has attracted substantial attention. In this study, multiple strategies were developed to create a sustainable green route for enhancing 7-DHC yield from kitchen waste by engineered Yarrowia lipolytica. Y. lipolytica strains were engineered and combined with various Δ24-dehydrocholesterol reductases. Overexpressing all the genes in the mevalonate pathway improved the precursor pool, increasing the 7-DHC titer from 21.8 to 145.6 mg/L. Additionally, optimizing medium components using the response surface method significantly raised the 7-DHC titer to 391.0 mg/L after shake flask cultivation. The engineered strain yielded a record 7-DHC titer of 3.5 g/L in a 5-L bioreactor when kitchen waste was used as a carbon source. Overall, these results demonstrate that engineered Y. lipolytica efficiently synthesizes 7-DHC from waste lipid feedstock, offering a promising route for its bioproduction.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"7 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Metabolic Pathways to Enhance Difucosyllactose Biosynthesis in Escherichia coli

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c09796

Shanquan Liang, Qi Quan, Dan Liu, Shaoqing Yang, Qiaojuan Yan, Zhengqiang Jiang

{"title":"Regulation of Metabolic Pathways to Enhance Difucosyllactose Biosynthesis in Escherichia coli","authors":"Shanquan Liang, Qi Quan, Dan Liu, Shaoqing Yang, Qiaojuan Yan, Zhengqiang Jiang","doi":"10.1021/acs.jafc.4c09796","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09796","url":null,"abstract":"Difucosyllactose (DFL), an important kind of fucosylated human milk oligosaccharides (HMOs), has garnered considerable attention due to its excellent physiological activities in infants. Previously, we achieved de novo biosynthesis of DFL; however, substantial residual intermediates of fucosyllactoses (FL) were detected. In this study, DFL biosynthesis was optimized, and residual FL were reduced by regulating metabolic pathways. Different plasmid combinations were used to regulate gene expression, achieving an optimal flux balance between 2′-FL and DFL. The expression level of key enzyme α-1,3-fucosyltransferase (α-1,3-FT, FucTa) was then enhanced by increasing plasmid copy number and integrating fucTa gene into the chromosome. Exocytosis of 2′-FL was reduced by deleting the sugar efflux transporter setA gene, thereby minimizing residual FL. Finally, strain BSF41 produced 55.3 g/L of DFL with only 2.59 g/L of residual FL in a 5 L fermentor, representing the highest reported titer to date. This study provides an important foundation for advancing the biosynthesis of fucosylated HMOs.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NRPS-like Gene LYS2 Contributed to the Biosynthesis of Cyclo(Pro-Val) in a Multistress-Tolerant Aromatic Probiotic, Meyerozyma guilliermondii GXDK6

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c08573

Ru Bu, Zhenze Li, Qiyu Qin, Huashan Bai, Can Meng, Ruihang Wei, Xinglin Chen, Shanguang Wu, Muhammad Kashif, Sheng He, Chengjian Jiang

{"title":"NRPS-like Gene LYS2 Contributed to the Biosynthesis of Cyclo(Pro-Val) in a Multistress-Tolerant Aromatic Probiotic, Meyerozyma guilliermondii GXDK6","authors":"Ru Bu, Zhenze Li, Qiyu Qin, Huashan Bai, Can Meng, Ruihang Wei, Xinglin Chen, Shanguang Wu, Muhammad Kashif, Sheng He, Chengjian Jiang","doi":"10.1021/acs.jafc.4c08573","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08573","url":null,"abstract":"Cyclo(Pro-Val) is a diketopiperazine (DKP) found widespread in marine microbes and resulting food products. With new bioactivities of cyclo(Pro-Val) being continually discovered, its potential applications in agriculture and food are becoming more evident, highlighting the need for efficient and practical methods to produce these compounds. However, the biosynthesis mechanisms of cyclo(Pro-Val), particularly in probiotics, remain unclear, and the functional identification of nonribosomal peptide synthases (NRPS) is still limited. This study presents a new efficient cyclo(Pro-Val) production system using the marine probiotic Meyerozyma guilliermondii (M. guilliermondii) GXDK6 as a chassis cell. We further characterized an NRPS-like gene, LYS2, which spans 3825 bp and encodes an l-2-amino-hexanedioic acid reductase (Lys2p) consisting of 1274 amino acids. A novel macrocyclization mechanism was revealed involving the peptide N-terminal and C-terminal imines mediated by Lys2p through nucleophilic reactions. Overexpressing LYS2 in GXDK6 resulted in a 45.5% increase in cyclo(Pro-Val) production, while knockout LYS2 completely halted its synthesis. This study revealed a novel metabolic regulatory pathway and biosynthetic mechanism for cyclo(Pro-Val) in marine yeast M. guilliermondii GXDK6. Refactoring the biosynthetic pathway in yeast through LYS2 provides an alternative approach for producing cyclo(Pro-Val).","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"46 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Gut Microbiota Composition and Microbial Phenolic Catabolism of Phenolic Compounds from Achillea millefolium L. and Origanum majorana L. Achillea millefolium L. 和 Origanum majorana L. 的酚类化合物对肠道微生物群组成和微生物酚类化合物分解代谢的调节作用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c07910

Irene Fernandez-Jalao, María de las Nieves Siles-Sánchez, Susana Santoyo, Alba Tamargo, Edgard Relaño de la Guía, Natalia Molinero, Victoria Moreno-Arribas, Laura Jaime

{"title":"Modulation of Gut Microbiota Composition and Microbial Phenolic Catabolism of Phenolic Compounds from Achillea millefolium L. and Origanum majorana L.","authors":"Irene Fernandez-Jalao, María de las Nieves Siles-Sánchez, Susana Santoyo, Alba Tamargo, Edgard Relaño de la Guía, Natalia Molinero, Victoria Moreno-Arribas, Laura Jaime","doi":"10.1021/acs.jafc.4c07910","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07910","url":null,"abstract":"The impact of the nonbioaccessible fraction of two phenolic-rich extracts from Achillea millefolium L. (yarrow) and Origanum majorana L. (marjoram) on the modulation of the human gut microbiota was investigated in vitro. Microbial metabolism of the phenolic compounds was also addressed. In general, phenolic acids or O-glycosidic flavones quickly disappeared, in contrast to methoxy- or C-glycosidic flavonoids. This colonic metabolism yielded phloroglucinol, 3,4-dimethoxyphenylacetic acid, 3-(4-hydroxyphenyl)-propionic acid, and 4-hydroxybenzoic acid as the main metabolites of the microbial catabolism of rosmarinic acid or caffeoylquinic acids, among others. The 16S rRNA gene sequencing showed that the most promising modulatory effect was related to the increase in Bifidobacterium spp., Collinsella spp., Romboutsia, and Akkermansia muciniphila for both plant extracts, along with Blautia spp. and Dialister for yarrow extract. This beneficial modulation was accompanied by the increase in butyric acid production, highlighting the potential prebiotic-like effect on the gut microbiota of these two previously unstudied edible plants.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"7 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rutin Synergizes with Colistin to Eradicate Salmonellosis in Mice by Enhancing the Efficacy and Reducing the Toxicity

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c06751

Yang Luo, Bing He, Zhi-Peng Li, Qin Zhong, Yu-Chen Liu, Hai-Yi Zhang, Yan Li, Hui-Lin Yan, Ya-Lin Hu, Zi-Jian Zheng, Hao Ren, Xiao-Ping Liao, Jian Sun

{"title":"Rutin Synergizes with Colistin to Eradicate Salmonellosis in Mice by Enhancing the Efficacy and Reducing the Toxicity","authors":"Yang Luo, Bing He, Zhi-Peng Li, Qin Zhong, Yu-Chen Liu, Hai-Yi Zhang, Yan Li, Hui-Lin Yan, Ya-Lin Hu, Zi-Jian Zheng, Hao Ren, Xiao-Ping Liao, Jian Sun","doi":"10.1021/acs.jafc.4c06751","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06751","url":null,"abstract":"The wide dissemination of multidrug-resistant (MDR) Gram-negative bacteria poses a significant global health and security concern. As developing new antibiotics is generally costly, fastidious, and time-consuming, there is an urgent need for alternative therapeutic strategies to address the gap in antibiotic discovery void. This study aimed to investigate the activity of colistin (CS) in combination with a natural product, rutin (RT), to combat against Salmonella Typhimurium (S. Tm) in vitro and in vivo. The results showed that a combination with RT enabled the potentiation of CS efficacy. Further mechanistic analysis indicated that RT disrupted iron homeostasis to inactivate the PmrA/PmrB system, thereafter reducing the bacterial membrane modifications for enhancing CS binding. Besides enhancing bactericidal activity of CS, RT was also observed to mitigate the CS-induced nephrotoxicity, by which the dosing limitation of CS was overcome for better pathogen clearance. The animal trial eventually confirmed the in vivo synergistic interaction of RT with CS to treat the bacterial infection. To sum up, the present study uncovered the potential of RT as a viable adjuvant of CS to eradicate the infection and protect the hosts, which might serve as a promising alternative to combat infections caused by MDR Gram-negative bacteria.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"88 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructing High-Yielding Serratia marcescens for (−)-α-Bisabolol Production Based on the Exogenous Haloarchaeal MVA Pathway and Endogenous Molecular Chaperones 基于外源卤代甲羟戊酸途径和内源分子伴侣蛋白构建高产的 marcescens 沙雷氏菌 (-)-α-Bisabol 生产系统

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c10135

Yao Lu, Di Liu, Long Wang, Yongai Ma, Tai-Ping Fan, Huaxiang Deng, Yujie Cai

{"title":"Constructing High-Yielding Serratia marcescens for (−)-α-Bisabolol Production Based on the Exogenous Haloarchaeal MVA Pathway and Endogenous Molecular Chaperones","authors":"Yao Lu, Di Liu, Long Wang, Yongai Ma, Tai-Ping Fan, Huaxiang Deng, Yujie Cai","doi":"10.1021/acs.jafc.4c10135","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10135","url":null,"abstract":"(−)-α-Bisabolol exhibits analgesic, anti-inflammatory, and skin-soothing properties and is widely applied in the cosmetic and pharmaceutical industries. The use of plant essential oil distillation or chemical synthesis to produce (−)-α-bisabolol is both inefficient and unsustainable. Currently, the microbial production of (−)-α-bisabolol mainly relies on Escherichia coli and Saccharomyces cerevisiae as chassis strains; however, high concentrations of (−)-α-bisabolol have certain toxicity to the strain. This study uses synthetic biology and metabolic engineering strategies to redesign a solvent-tolerant Serratia marcescens for the efficient production of (−)-α-bisabolol. By introducing the Haloarchaea-type mevalonate (MVA) pathway and the (−)-α-bisabolol biosynthesis pathway, we successfully constructed a strain capable of producing (−)-α-bisabolol. The coexpression of the chaperone protein DnaK/J significantly enhanced the soluble expression of the (−)-α-bisabolol synthase, resulting in a 10% increase in (−)-α-bisabolol titer. Furthermore, knockout of the PhoA gene, which reduced the formation of the byproduct farnesol (FOH), further increased the (−)-α-bisabolol titer to 3.21 g/L. In a 5 L bioreactor, S. marcescens achieved a final (−)-α-bisabolol titer of 30.2 g/L, representing the highest titer reported to date. This research provides guidance for the production of (−)-α-bisabolol in nonmodel microorganisms without the requirement for induction.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"89 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Talarergosteroids A–C: Three Unusual Steroid-Polyketone Conjugates with Antifungal Activity from a Kandelia Obovata Derived Fungus Talaromyces sp

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2024-12-19 DOI: 10.1021/acs.jafc.4c10156

Jialin Li, Zirong Lin, Haiqi Zeng, Jiechang Zeng, Siyao Ye, Chen Chen, Hao Jia, Kang Li, Zhigang She, Yuhua Long

{"title":"Talarergosteroids A–C: Three Unusual Steroid-Polyketone Conjugates with Antifungal Activity from a Kandelia Obovata Derived Fungus Talaromyces sp","authors":"Jialin Li, Zirong Lin, Haiqi Zeng, Jiechang Zeng, Siyao Ye, Chen Chen, Hao Jia, Kang Li, Zhigang She, Yuhua Long","doi":"10.1021/acs.jafc.4c10156","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10156","url":null,"abstract":"Three previously undescribed steroid–polyketone conjugates, talarergosteroids A–C (1–3), together with talarergosteroid D (4), which was first identified from a natural source, were isolated from a Kandelia Obovata derived fungus Talaromyces sp. SCNU-F0041. Compounds 1 and 2 bear a complicated 6/6/6/5/6/6 hexacyclic ring system characterized by an oxaspiro[5.5]undecane architecture. Compound 3 possesses a benzofuran moiety substituted at C-3 in ergosterol. The structures of the new compounds were identified by comprehensive spectroscopic analysis, X-ray diffraction, and electronic circular dichroism (ECD) calculation. Talarergosteroid B (2) showed significant inhibitory activity against the agricultural plant pathogen Fusarium oxysporum f. sp. lycopersici (MIC = 0.78 μg/mL), outperforming the positive control carbendazim (MIC = 1.56 μg/mL). Preliminary research disclosed that compound 2 may inhibit the spore germination progress, malform the fungal mycelium, and damage the organelle. These results indicate that compound 2 could be a potential fungicidal lead compound against Fusarium oxysporum f. sp. lycopersici.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0