Journal of Agricultural and Food Chemistry最新文献

{"title":"The Neuroprotective Effects of Primary Functional Components Mulberry Leaf Extract in Diabetes-Induced Oxidative Stress and Inflammation","authors":"Zi-Xiang Lin, Chau-Jong Wang, Hsin-Wei Tu, Meng-Ting Tsai, Meng-Hsun Yu, Hui-Pei Huang","doi":"10.1021/acs.jafc.4c09422","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09422","url":null,"abstract":"Diabetes-associated neurodegeneration may result from increased oxidative stress in the brain under hyperglycemic conditions, which leads to neuronal cell death. The current study employs the neuroblastoma cell line SH-SY5Y and db/db mouse model of diabetes maintained on a high-fat diet to investigate the neuroprotective effects of the primary functional components of mulberry (Morus alba Linn) leaf extract (MLE), chlorogenic acid (CGA), and neochlorogenic acid (NCGA). CGA and NCGA demonstrated the ability to enhance the activities of the antioxidant enzymes superoxide dismutase and glutathione peroxidase, and attenuate inflammation via regulating nuclear factor erythroid 2-related factor 2 (Nrf2), nuclear factor-κB (NFκB), and inflammatory cytokines, thereby protecting SH-SY5Y cells from oxidative damage induced by palmitic acid and high glucose. CGA and NCGA were found to decrease the expression of proinflammatory proteins α-synuclein and amyloid-β (Aβ). In addition, CGA and NCGA treatments increased the expression of tyrosine hydroxylase (TH) and brain-derived neurotrophic factor (BDNF). Furthermore, MLE supplementation in the animal model resulted in decreased levels of α-synuclein and Aβ concomitant with an elevated expression of TH. These experimental findings suggest that the neuroprotective effects of CGA and NCGA may be mediated via three pathways: reducing oxidative stress, decreasing neuronal inflammation, and enhancing BDNF expression.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"61 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function","authors":"Shu Liu, Yuhang Jin, Wenyi Zhou, Juxiong Liu, Liqun Tu, Yufei Zhang, Meng Zhang, Xuanting Liu, Yu Cao, Wenjin Guo, Shoupeng Fu","doi":"10.1021/acs.jafc.4c10238","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10238","url":null,"abstract":"Mastitis in dairy cows is an inflammatory disease that severely affects the health and lactation functions of dairy cows. Mitochondrial damage is closely related to the inflammatory response. How to effectively alleviate mitochondrial damage is the key to preventing and treating mastitis in dairy cows. In this study, we found elevated levels of inflammatory response and mitochondrial damage accompanied by reduced expression of Sirt5 (Sirtuin5) in cows with mastitis compared with healthy cows. This suggests that Sirt5 plays an important role in mastitis in dairy cows. Subsequently, we further analyzed mammary gland tissue from healthy and mastitis cows by untargeted metabolomics (LC-MS/MS) and screened for the differential metabolite <span>l</span>-glutamic acid (<span>l</span>-Glu). To further validate the effect of <span>l</span>-Glu on mastitis in dairy cows, we conducted a study using MAC-T cells. The results showed that <span>l</span>-Glu was able to ameliorate LPS-induced mitochondrial damage by activating Sirt5 and promoting mitochondrial fusion and the upregulation of mitochondrial membrane potential (MMP) levels. In contrast, <span>l</span>-Glu was unable to protect mitochondrial function after knocking down Sirt5. Furthermore, we found that <span>l</span>-Glu was able to upregulate the expression of nuclear factor E2-related factor (Nrf2) and peroxiredoxin 1 (Prdx1) in LPS-induced MAC-T cells, and promoted the entry of Nrf2 into the nucleus, which was reversed by knocking down Sirt5. Next, we further explored whether <span>l</span>-Glu alleviates mitochondrial damage through the Nrf2/Prdx1 signaling axis by using the Nrf2 inhibitor RA. The results showed that the use of RA promoted LPS-induced mitochondrial damage and blocked the protective effect of <span>l</span>-Glu on mitochondrial function. In conclusion, <span>l</span>-Glu ameliorates mitochondrial damage by targeting Sirt5 to activate the Nrf2/Prdx1 signaling axis and alleviate mastitis in dairy cows. This study provides a new target and theoretical basis for the clinical control of mastitis. <span>l</span>-Glu could be added as a dietary supplement to the diets of dairy cows and maintain mammary gland homeostasis, thereby protecting the health and economic value of dairy cows.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"38 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing the Bitterness of Rapeseed Protein: Integrating Enzymatic Treatment, Metabolomics, and Sensory Analysis to Elucidate Underlying Mechanisms","authors":"Andrea Spaccasassi, Christoph Walser, Anni Nisov, Nesli Sozer, Oliver Frank, Corinna Dawid, Thomas F. Hofmann","doi":"10.1021/acs.jafc.4c10442","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10442","url":null,"abstract":"Rapeseed products, such as protein concentrates, hold promise for addressing global protein demands, but their application in food products is limited by their bitter and astringent taste. This study investigates the use of β-glucosidase (BG) and laccase (LAC) enzymatic treatment, individually and combined, to enhance the flavor of rapeseed protein concentrate (RPC). Untargeted metabolomics and sensory analysis reveal that LAC reduces the bitter compound kaempferol 3-<i>O</i>-(2‴-<i>O</i>-sinapoyl-β-D-sophoroside) (K3OSS) as well as a general reduction in other phenolic compounds, which correlates with a significant decrease in bitterness and astringency. In contrast, BG treatment elevates the levels of K3OSS and is accompanied by increased bitterness due to the conversion of precursor compounds to K3OSS. In addition, the synergistic use of both enzymes significantly reduces the concentration of K3OSS, resulting in a lower perception of bitterness. The LC–MS analysis of pure reference compounds treated with LAC and BG confirms that BG-mediated treatment facilitates the breakdown of larger kaempferol glycosides into K3OSS, while LAC treatment promotes polyphenol polymerization. Consequently, LAC treatment seems to be an effective strategy to improve the sensory quality of RPC and make it more suitable for human consumption.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"36 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Escherichia coli Platform for Cannabinoid Precursor Olivetolic Acid Biosynthesis from Inexpensive Inputs","authors":"Xinwei Yang, Wenhao Liang, Xinyi Lin, Mingyue Zhao, Qinshu Zhang, Yong Tao, Jianzhong Huang, Chongrong Ke","doi":"10.1021/acs.jafc.4c11867","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11867","url":null,"abstract":"Olivetolic acid (OLA), an initial precursor of cannabinoids, is catalyzed by type III polyketide synthase, which has a wide range of pharmacological activities, such as antimicrobial and cytotoxic effects. Here, we applied systematic metabolic engineering to develop a multienzyme cascade system to produce OLA via two low-cost inputs. The polyketide synthase (OLS) and cyclase enzymes (OAC), along with the best combination of hexanoyl-CoA and malonyl-CoA synthetases (AEE3 and MatB), were first introduced into the biocatalytic system to increase the supply of hexanoyl-CoA and malonyl-CoA as starting and extender units. To drive the catalysis smoothly, an ATP regeneration system and a CoA-sufficient supply system were incorporated into the biocatalysts to provide enough cofactors. Furthermore, malonyl-CoA flux was redirected to OLA biosynthesis through delicate control of the fatty acid biosynthesis (FAB) pathway via promoter engineering. Collectively, these strategies have led us to produce OLA at a titer of 102.1 mg/L with a productivity of 25.5 mg/L/h by using malonate and hexanoate as direct substrates. Our biocatalytic system provides an effective platform for the production of the cannabinoid precursor OLA in <i>Escherichia coli</i> and may be a valuable reference for the development of microbial cell factories that use hexanoyl-CoA and malonyl-CoA as important intermediates.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"8 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of Sesterdiaporate, an Antifungal Sesterterpene Lactone, against Penicillium italicum, Possibly via Inhibition of Glycosylphosphatidylinositol Biosynthesis from Diaporthe sp. HT-79","authors":"Liangliang Gao, Huiting Lin, Yan Liang, Mingnan You, Lijian Ding, Fangjian Feng, Qin Xiong, Yueming Jiang, Jun Zhang","doi":"10.1021/acs.jafc.4c12887","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12887","url":null,"abstract":"The present study reported the discovery of sesterdiaporate (SES), a structurally unique antifungal sesterterpene lactone from <i>Diaporthe</i> sp. HT-79. SES exhibited a pronounced inhibitory effect on spore germination and mycelial growth of <i>Penicillium italicum</i>, with median effective concentrations (EC₅₀) of 0.37 and 0.079 μg/mL, respectively. This inhibitory effect was significantly more pronounced than that observed with prochloraz (0.74 and 6.83 μg/mL, respectively). Treatment with SES resulted in the swelling of both spores and mycelia of <i>P. italicum</i> as well as an increase in cell membrane permeability. Further investigation suggested that SES may disrupt glycosylphosphatidylinositol biosynthesis, ultimately leading to cell death. <i>In vivo</i> tests on navel orange fruit showed that SES was effective in reducing the diameter of lesions caused by <i>P. italicum</i>, with a lesion inhibition percentage of 49.4% at a concentration of 3.7 μg/mL, which was significantly superior to that of prochloraz (14.2% at the same concentration).","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"31 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Regulation of the l-Proline Pathway for Efficient l-Arginine Production in Escherichia coli","authors":"Lijuan Wang, Kun Yang, Zhi Zhang, Weijie Ye, Yingying Guo, Haonan Yu, Zhiqiang Liu, Yuguo Zheng","doi":"10.1021/acs.jafc.4c07829","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07829","url":null,"abstract":"<span>l</span>-Arginine, a semiessential amino acid crucial for human health, has broad applications in cosmetics, nutraceuticals, feed, and pharmaceuticals. In this study, we developed an <i>Escherichia coli</i> strain with enhanced <span>l</span>-arginine production by deregulating negative feedback, enhancing the synthesis pathway, and increasing precursor and cofactor availability. The engineered strain achieved titers of 6.41 g/L in shake flasks and 63.9 g/L with a yield of 0.31 g/g of glucose in a 5 L fermenter. Blocking the competitive <span>l</span>-proline synthesis pathway elevated the <span>l</span>-arginine titer to 9.36 g/L but reduced the biomass. To fine-tune <span>l</span>-proline synthesis without exogenous <span>l</span>-proline, we developed a dynamic regulatory method for <i>proB</i> gene control. The final strain, harboring <i>proB</i> driven by a temperature-sensitive promoter, achieved 65.6 g/L <span>l</span>-arginine with a yield of 0.42 g/g glucose in a 5 L fermenter. Balancing growth and production through dynamic regulation of the <span>l</span>-proline pathway presents a viable strategy for refining <span>l</span>-arginine bioproduction.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"207 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Characterization and Mechanism of Thermostability of the Thermophilic Hyaluronate Lyase TcHly8D","authors":"Yuzhu Zhang, Hao Wu, Zheng Fu, Shilong Zhang, Meiling Zheng, Jiaxia Sun, Zhongxia Lu, Rilei Yu, Wengong Yu, Feng Han","doi":"10.1021/acs.jafc.4c09901","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09901","url":null,"abstract":"Hyaluronate lyases are widely used in medicine and biochemical engineering and are also applied as a tool enzyme to prepare oligosaccharides with various biological activities. To date, only a few hyaluronate lyases are on sale with poor thermostability. In this study, a PL8 hyaluronate lyase, TcHly8D, was found from <i>Thermasporomyces composti</i> and expressed in <i>Escherichia coli</i> with a maximum yield of 1.77 × 10⁹ U/L (3.14 g/L) in a 5-L bioreactor. The recombinant TcHly8D exhibited a high hyaluronate lyase activity of 5.64 × 10⁵ U/mg and an excellent thermostability with half-lives of 184.9 h at 60 °C. Fifty micrograms of TcHly8D could catalyze 5 g of hyaluronic acid with an oligosaccharide yield of 84.8% in 4 h. The salt bridges, hydrogen bonds, and proline residues, but not disulfide bonds, played important roles in the thermostability of TcHly8D. These findings provide insights into the multifunctional application potential of TcHly8D in agriculture, medicine, and the food industry.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"123 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purple Sweet Potato Ameliorates High-Fat Diet-Induced Visceral Adiposity by Attenuating Inflammation and Promoting Adipocyte Browning","authors":"Chi-Hua Yen, Ming-Hui Chiang, Yi-Chen Lee, Erl-Shyh Kao, Huei-Jane Lee","doi":"10.1021/acs.jafc.4c08799","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08799","url":null,"abstract":"Accumulation of visceral fat has been reported to increase systemic inflammation. Purple sweet potato (<i>Ipomoea batatas</i> L., PSP), known for its anthocyanin content, potentiates in mitigating oxidative stress. This study aimed to investigate the underlying mechanisms by which PSP influences body fat deposition. Five-week-old male Sprague–Dawley rats (<i>n</i> = 5) were fed a 43% high-fat diet (HFD) for 2 weeks to induce obesity, followed by 19 weeks of HFD supplemented with 5% PSP. PSP significantly improved body weight and reduced visceral fat mass and adipocyte size. In visceral and subcutaneous adipose tissues, PSP significantly downregulated proteins of FAS, ACC1, and PPARγ with inflammatory markers TNF-α, IL-6, and MCP-1. PSP reduced the proteins of inflammasome components, NLRP3, caspase-1, IL-1β, and HIF-1α. PSP increased the proteins associated with adipose tissue browning, FNDC5, PGC-1α, and UCP-1, particularly in visceral adipose tissue. In conclusion, PSP effectively reduced visceral fat accumulation, attenuated inflammation, and promoted adipocyte browning.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid On-Site and Sensitive Detection of Microplastics Using Zirconium(IV)-Assisted SERS Label","authors":"Haoming Yang, Haoxin Ye, Song Yan, Chu Chu, Tianxi Yang","doi":"10.1021/acs.jafc.4c08934","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08934","url":null,"abstract":"Microplastics have emerged as significant pollutants in terrestrial and marine ecosystems, with their accumulation posing a threat to human health through biomagnification along the food chain. Developing a rapid, on-site, and sensitive method for detecting microplastics in agri-food and environmental systems is important for assessing and minimizing their potential risks. In this study, we developed a novel surface-enhanced Raman spectroscopy (SERS) technique for the rapid, on-site, and ultrasensitive detection of microplastics. Our innovative technique incorporated Zr⁴⁺-assisted SERS label strategies, utilizing rhodamine B as a Raman reporter to improve microplastics analysis. By utilizing Zr⁴⁺-assisted SERS label approaches, we can achieve qualitative and ultrasensitive quantification of 10 μm polystyrene microplastics (PSMPs) at concentrations as low as 0.1 ppm with a detection limit of 1 ppb. Furthermore, this approach allows for detecting microplastics in real-world scenarios, with recovery rates exceeding 90% for polystyrene microplastic concentrations ranging from 5 to 30 ppm in tap water systems. When integrated with a portable Raman spectrometer, this innovative approach showcases the rapid, on-site, accurate, and sensitive detection of microplastics and has great potential for analyzing various types of microplastics in agri-food and environmental systems.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"30 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Whey–Plant Protein Heteroprotein Systems with Synergistic Properties and Versatile Applications","authors":"Chang Liu, Ruhua Wang, Tianyang Wang, Chunkai Gu, Lingling Zhang, Demei Meng, Mingfei Pan, Rui Yang","doi":"10.1021/acs.jafc.4c10736","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c10736","url":null,"abstract":"Combining animal protein with plant protein is a feasible approach to provide heteroprotein formulations with versatile properties. This review introduces the interactions of typical protein whey protein (WP) from milk with soy protein (SP), pea protein (PP), rapeseed protein (RAP), lupine protein (LP), and rice protein (RIP) through physical and chemical methods. The characteristics of whey–plant protein complexes are described with particular emphasis on the protein types, structures, and properties. In addition, the factors that influence the formation of whey–plant complexes are reviewed. The potential food applications of whey–plant protein complexes are reviewed. Overcoming the shortcomings and future challenges for applications of the heteroprotein in the food field are highlighted. This review will fill the gap of whey protein and are important for the development of more versatile properties of whey proteins as well as a systematic understanding of the synergistic biological roles of these active proteins.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}