Journal of Agricultural and Food Chemistry最新文献_第3页

In Vivo Protein Digestibility and Metabolic Differences of Muscle from Three Shrimp and Crab Species 三种虾蟹肌肉体内蛋白质消化率及代谢差异

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-08 DOI: 10.1021/acs.jafc.6c09843

Mingyu Yin,Wenyu Gao,Yulong Zhao,Xutao Li,Hao Li,Xiaotong Huo,Zhou Fang,Qingqing Jiang,Yinci Xi,Xichang Wang

{"title":"In Vivo Protein Digestibility and Metabolic Differences of Muscle from Three Shrimp and Crab Species","authors":"Mingyu Yin,Wenyu Gao,Yulong Zhao,Xutao Li,Hao Li,Xiaotong Huo,Zhou Fang,Qingqing Jiang,Yinci Xi,Xichang Wang","doi":"10.1021/acs.jafc.6c09843","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c09843","url":null,"abstract":"This study compared the digestive utilization and metabolic responses of muscle proteins from Eriocheir sinensis (EM), Litopenaeus vannamei (SM), and Procambarus clarkii (LM) in a rat model. Nitrogen digestibility was assessed by the titanium dioxide indicator method, combined with serum biochemistry, renal histology, intestinal particle size, and metabolomics. All protein groups significantly increased body weight vs protein-free control, with EM showing the greatest growth and protein efficiency ratio. Digestibility did not differ among the three groups. EM had the highest apparent ileal digestibility; EM and SM had similar true ileal digestibility, both higher than LM. Metabolomics identified 2,072 metabolites, enriched in amino acid and protein digestion pathways. Species-specific differences in lipid and antioxidant pathways: EM affected arachidonic acid, LM modulated linoleic acid, and SM involved ascorbate. Both SM and LM showed reduced amino acid utilization. These findings provide a mechanistic basis for refined evaluation and utilization of crustacean proteins.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"41 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Tissue-Inspired Organic–Inorganic Hybrid Network for Hot-Pressed Peanut Meal Protein-Based Adhesives with High Water Resistance 骨组织激发的有机-无机混合网络用于热压花生粕蛋白基高耐水性粘合剂

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-07 DOI: 10.1021/acs.jafc.6c11684

Xiaoshuai Han,Chenrui Ouyang,Wei Tian,Jiangbo Wang,Zhixin Jia,Shaohua Jiang

引用次数: 0

Spatial Mass Spectrometry Imaging Combined with Multiomics Reveals the Spatiotemporal Distribution of Monascus-Associated Metabolites during the Ripening of Monascus-Fermented Cheese 空间质谱成像结合多组学揭示红曲霉发酵奶酪成熟过程中红曲霉相关代谢物的时空分布

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-07 DOI: 10.1021/acs.jafc.6c08592

Yadong Wang,Jiajie Wang,Zirui Guo,Jing Dong,Xiaochun Yang,Xiaodong Li,Yanbo Wang,Bei Wang

{"title":"Spatial Mass Spectrometry Imaging Combined with Multiomics Reveals the Spatiotemporal Distribution of Monascus-Associated Metabolites during the Ripening of Monascus-Fermented Cheese","authors":"Yadong Wang,Jiajie Wang,Zirui Guo,Jing Dong,Xiaochun Yang,Xiaodong Li,Yanbo Wang,Bei Wang","doi":"10.1021/acs.jafc.6c08592","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08592","url":null,"abstract":"Monascus-fermented cheese (MC) is a dairy product with distinctive red coloration and Monascus-associated metabolites. The spatial distribution patterns and associated metabolic characteristics of metabolites within the cheese matrix remain unclear. In this study, a multiomics strategy integrating matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), metabolomics, metagenomics, and proteomics was applied to investigate the spatiotemporal dynamics of Monascus-associated metabolites during MC ripening. Metabolomic analysis revealed ripening-associated metabolic changes, and the increasing trend of monacolin K during ripening was quantified by targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, γ-aminobutyric acid, monascin, and monascorubramine-related ion signals were putatively annotated as Monascus-associated metabolic features and exhibited heterogeneous spatial distributions in MSI analysis. Metagenomics revealed the presence of Monascus and gene potential associated with Monascus-associated metabolite metabolism. Proteomics revealed temporal changes in candidate protein abundances associated with metabolite-related processes. This study provides multiscale insights into spatial metabolic patterns in MC.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Acid Adaptability of Zearalenone Lactonase Guided by an Integrated Structural and Computational Strategy 基于集成结构与计算策略的玉米赤霉烯酮内酯酶工程酸适应性研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-07 DOI: 10.1021/acs.jafc.6c11085

Binbin Ouyang,Zhaolin Huang,Chenshuo Song,Dejing Yin,Dawei Ni,Wenli Zhang,Yijian Rao,Wei Xu,Wanmeng Mu

{"title":"Engineering Acid Adaptability of Zearalenone Lactonase Guided by an Integrated Structural and Computational Strategy","authors":"Binbin Ouyang,Zhaolin Huang,Chenshuo Song,Dejing Yin,Dawei Ni,Wenli Zhang,Yijian Rao,Wei Xu,Wanmeng Mu","doi":"10.1021/acs.jafc.6c11085","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11085","url":null,"abstract":"Zearalenone (ZEN) lactonases are promising biocatalysts for detoxifying ZEN-contaminated food and feed. However, their poor activity and stability under acidic conditions limit practical applications. Here, we establish an integrated computationally/structurally guided hydrophobic microenvironment engineering as a paradigm for improving the acid adaptability of ZEN lactonases. Constant pH molecular dynamics (CpHMD) simulations revealed that acidic conditions suppress proton abstraction by altering catalytic-residue protonation and destabilizing key structural regions. Structure-guided engineering introduced Pro and Phe residues to stabilize acid-sensitive regions, while hydrophobic microenvironment engineering promoted substrate repositioning and the formation of near-attack conformations. The engineered M4 mutant exhibited a 111.7-fold enhancement in acid stability. Validation with 5Z-7-oxozeaenol supported a hydrophobic gating mechanism, in which the hydrophobic microenvironment governs substrate positioning, aligns catalytic residues, and stabilizes reactive conformations. M4 degraded approximately 70% of ZEN under simulated gastrointestinal pH conditions, demonstrating its potential for ZEN detoxification.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"6 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Bacillus megaterium Reshapes Rhizosphere Processes to Promote Phthalate Esters Dissipation 内生巨芽孢杆菌重塑根际过程促进邻苯二甲酸酯耗散

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-06 DOI: 10.1021/acs.jafc.6c11259

Hong-Jia Huang,Xin Chen,Dong-Yang Zhou,Cai-Qin Cheng,Lei Xiang,Yan-Wen Li,Hai-Ming Zhao,Quan-Ying Cai,Da-Wei Li,Nai-Xian Feng,Ce-Hui Mo

{"title":"Endophytic Bacillus megaterium Reshapes Rhizosphere Processes to Promote Phthalate Esters Dissipation","authors":"Hong-Jia Huang,Xin Chen,Dong-Yang Zhou,Cai-Qin Cheng,Lei Xiang,Yan-Wen Li,Hai-Ming Zhao,Quan-Ying Cai,Da-Wei Li,Nai-Xian Feng,Ce-Hui Mo","doi":"10.1021/acs.jafc.6c11259","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c11259","url":null,"abstract":"Phthalate esters (PAEs) contaminate agricultural soils, yet how introduced endophytes reshape rhizosphere processes and influence PAE dissipation remains unclear. We investigated the PAE-degrading endophyte Bacillus megaterium YJB3 in choysum (Brassica parachinensis L.) grown in soils spiked with dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP). YJB3 alleviated PAE-induced inhibition of lateral-root development and enhanced PAE dissipation, particularly DEHP dissipation under low contamination. Soil zymography revealed YJB3-associated shifts in the spatial distributions of β-glucosidase and xylanase activities around roots, with an increased proportion of β-glucosidase hotspots within the total enzyme-active area under PAE stress. Across the rhizosphere, rhizoplane, and endosphere, YJB3 inoculation was associated with changes in bacterial community composition and higher relative abundances of several genera, including Streptomyces, Nocardioides, and Kitasatospora. Collectively, YJB3-associated enhancement of PAE dissipation coincided with changes in root development, rhizosphere enzyme activities, and root-associated bacterial communities, highlighting the potential of endophytes for remediation of PAE-contaminated soils.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"7 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cinnamon Polysaccharide Regulates FUNDC1-Dependent Mitochondrial Autophagy to Reduce Oxidative Stress-Driven Mammary Gland Aging in Dairy Cows 肉桂多糖调节fundc1依赖性线粒体自噬，降低奶牛氧化应激驱动的乳腺衰老

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-06 DOI: 10.1021/acs.jafc.6c03838

Meng Zhang,Zimo Pei,Hongbin Yin,Wenyi Zhou,Yu Cao,Huijie Hu,Liqun Tu,Xuanting Liu,Guiqiu Hu,Juxiong Liu,Wenjin Guo,Shoupeng Fu

{"title":"Cinnamon Polysaccharide Regulates FUNDC1-Dependent Mitochondrial Autophagy to Reduce Oxidative Stress-Driven Mammary Gland Aging in Dairy Cows","authors":"Meng Zhang,Zimo Pei,Hongbin Yin,Wenyi Zhou,Yu Cao,Huijie Hu,Liqun Tu,Xuanting Liu,Guiqiu Hu,Juxiong Liu,Wenjin Guo,Shoupeng Fu","doi":"10.1021/acs.jafc.6c03838","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c03838","url":null,"abstract":"Mastitis causes oxidative stress, inflammation, mitochondrial dysfunction, and impaired lactation in dairy cows. This study investigated whether FUNDC1 (FUN14 domain-containing protein 1)-associated mitochondrial quality control protects mammary tissue from oxidative stress-driven senescence and whether cinnamon polysaccharide (CP) can restore this pathway during mastitis recovery. Mastitis-associated oxidative stress reduced FUNDC1 expression, disrupted mitochondrial dynamics by suppressing fusion and promoting fission, decreased mitochondrial membrane potential, and increased senescence markers in bovine mammary epithelial cells. FUNDC1 overexpression alleviated mitochondrial dysfunction and senescence-associated changes. Mechanistically, the E3 ubiquitin ligase FANCL contributed to the proteasome-dependent reduction of FUNDC1 under oxidative stress. CP treatment restored FUNDC1 expression, reduced inflammation and oxidative stress, improved mitochondrial homeostasis, and enhanced postmastitis milk-yield recovery when combined with antibiotic treatment. These findings identify the FANCL–FUNDC1 axis as a link between oxidative stress and mammary gland senescence and support CP as a functional adjunct for mastitis management.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"75 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of a Cytochrome P450 for Efficient 11α-Hydroxylation of Steroids by Integrating Genome Mining and Global Dynamic Simulations 整合基因组挖掘和全局动态模拟的细胞色素P450高效11α-羟化类固醇的合理设计

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-06 DOI: 10.1021/acs.jafc.6c10096

Yuyao Du,Tianmeng Xie,Ming Sun,Xun Chen,Weidong Liu,Fuping Lu,Hui-Min Qin,Shuhong Mao

{"title":"Rational Design of a Cytochrome P450 for Efficient 11α-Hydroxylation of Steroids by Integrating Genome Mining and Global Dynamic Simulations","authors":"Yuyao Du,Tianmeng Xie,Ming Sun,Xun Chen,Weidong Liu,Fuping Lu,Hui-Min Qin,Shuhong Mao","doi":"10.1021/acs.jafc.6c10096","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c10096","url":null,"abstract":"The 11α-hydroxylation of steroids is essential for their semisynthesis. However, natural 11α-hydroxylases are scarce and generally exhibit low catalytic activity. In this study, a structure–function-guided computational approach was applied to identify a cytochrome P450 monooxygenase (CosaP450) with 11α-hydroxylation activity. By modulating the flexibility of the gating helix (N62-E75), the engineered variant MT3 (T72Q/F70T/Y226Q) increased the production of 11α,17α-hydroxyprogesterone (11α,17α-OHP) from 680 mg/L to 1140 mg/L. Then, the targeted engineering of the substrate channel and active site was employed based on the multiscale MD simulations, and the mutant MT6 (T72Q/F70T/Y226Q/V374A/G137T/E118G) achieved a yield of 1863 mg/L. MD analysis revealed that V374A relieved channel steric hindrance, while G137T/E118G remodeled the hydrogen-bond network to stabilize the binding conformation. Redox partner engineering further enhanced the titer of MT6 AnCPR to 2489 mg/L. This work establishes a computational framework for rational discovery and engineering of efficient 11α-hydroxylases, providing a promising strategy for enzymatic steroid functionalization.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Food-Grade Kappa-Carrageenan Induces Liver Injury through the FXR/FGF15 Pathway in Mice 食品级kappa - carragean通过FXR/FGF15通路诱导小鼠肝损伤

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-06 DOI: 10.1021/acs.jafc.6c07572

Suping Zhu,Xiaojing Guo,Hengjun Du,Minna Luo,Hang Xiao,Yanhui Han

引用次数: 0

Alternative Splicing Isoforms of FgSdhC1 Differentially Modulate Intrinsic SDHI Resistance and Biological Fitness in Fusarium graminearum FgSdhC1的不同剪接异构体差异调节小麦镰刀菌内在的SDHI抗性和生物适应性

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-06 DOI: 10.1021/acs.jafc.6c08554

Xiujuan Li,Siqi Yao,Wei Wang,Shuzhou Liu,Guo Li,Bin Chen,Jiawei Li,Wenchan Chen,Xiushi Song,Wenyong Shao,Changjun Chen

{"title":"Alternative Splicing Isoforms of FgSdhC1 Differentially Modulate Intrinsic SDHI Resistance and Biological Fitness in Fusarium graminearum","authors":"Xiujuan Li,Siqi Yao,Wei Wang,Shuzhou Liu,Guo Li,Bin Chen,Jiawei Li,Wenchan Chen,Xiushi Song,Wenyong Shao,Changjun Chen","doi":"10.1021/acs.jafc.6c08554","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c08554","url":null,"abstract":"Alternative splicing (AS) of FgSdhC1 was identified in SDHI-sensitive (FgS) and intrinsically resistant (FgR) Fusarium graminearum isolates, generating four AS isoforms in each background. Isogenic replacement assays showed that individual isoforms differentially altered sensitivity to pydiflumetofen, boscalid, and fluopyram and affected multiple fitness-related traits. The average compound fitness index of FgR-derived isoform replacement strains was 0.54, compared with 0.20 for FgS-derived strains, and FgRAD2-C showed the highest value (1.43). FgSIR and FgSAD3 transcripts were significantly more abundant in FgS-C than the corresponding FgR-derived isoforms in FgR-C. Isoform transcript abundance, SDH activity, carbon source utilization, and responses to environmental stresses also differed among replacement strains. Transcriptomic and RT-qPCR analyses identified FgPRPF4B, FgDHX38/FgPRP16, and FgPPIL1 as differentially expressed candidate splicing-related genes. Collectively, these findings showed that FgSdhC1 alternative splicing isoforms differentially modulate intrinsic SDHI resistance and biological fitness in F. graminearum.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"13 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Pichia pastoris for De Novo Biosynthesis of D-Lactic Acid from Methanol 毕赤酵母甲醇从头合成d -乳酸的代谢工程研究

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2026-11-05 DOI: 10.1021/acs.jafc.6c06547

Chenmeng Zhang,Yuncong Wang,Xun Wang,Yu Zhang,Xun Li,Fei Wang

{"title":"Metabolic Engineering of Pichia pastoris for De Novo Biosynthesis of D-Lactic Acid from Methanol","authors":"Chenmeng Zhang,Yuncong Wang,Xun Wang,Yu Zhang,Xun Li,Fei Wang","doi":"10.1021/acs.jafc.6c06547","DOIUrl":"https://doi.org/10.1021/acs.jafc.6c06547","url":null,"abstract":"D-Lactic acid (D-LA) is an essential chiral monomer for polylactic acid (PLA), widely used in biodegradable food packaging and agricultural materials. Conventional sugar-based fermentation is constrained by competition with food resources and price volatility, highlighting the need for sustainable alternatives. Methanol, a renewable and cost-effective C1 substrate, provides thermodynamic advantages for D-LA biosynthesis but remains underutilized. In this study, Pichia pastoris was modified by systematic metabolic engineering to create an efficient methanol-D-LA conversion pathway. By reducing D-LA degradation, enhancing methanol utilization, improving acid tolerance, and eliminating competing pathways, the optimized fed-batch process achieved a D-LA titer of 12.05 g/L. Sodium citrate was employed as an auxiliary metabolic regulator to support central metabolism and process robustness. This work demonstrates methanol bioconversion as a sustainable route for D-LA production and establishes P. pastoris as a promising platform for manufacturing biobased monomers and high-value chemicals from renewable C1 feedstocks.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149359935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0