Journal of Agricultural and Food Chemistry最新文献

{"title":"Unraveling Time-Resolved Transcriptional and Metabolic Shifts in the Mixed Fermentation of Saccharomyces cerevisiae and Hanseniaspora uvarum","authors":"Qing Sun, Peng An, Peiyang Li, Hao Wang, Shiheng Tao, Yanlin Liu","doi":"10.1021/acs.jafc.5c00722","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00722","url":null,"abstract":"Wine fermentation and flavor formation are shaped by complex biochemical reactions driven by a variety of microorganisms. Non-Saccharomyces yeasts, such as <i>Hanseniaspora uvarum</i> (HU), are often used in mixed fermentation with <i><i>Saccharomyces cerevisiae</i></i> (SC) to enhance wine aroma. However, the lack of systematic knowledge regarding transcriptional responses and metabolic behaviors during fermentation has hindered the rational control of the mixed fermentation processes. To address this, we investigated transcriptional dynamics and metabolic behavior throughout the entire fermentation process, with a particular focus on the roles of microbial metabolism in flavor formation during mixed fermentation with HU. At the transcriptional level, the addition of HU led to significant changes in SC’s gene expression, particularly in pathways related to glyoxylate and dicarboxylate metabolism, pyruvate metabolism, and amino sugar and nucleotide sugar metabolism. Furthermore, using genome-scale metabolic modeling, we uncovered key metabolic strategies employed by the two strains in mixed fermentation. These include distinct sugar utilization patterns, ethanol production, fatty acid metabolism, and central carbon allocation strategies. Notably, we identified two metabolic bypasses, from dihydroxyacetone phosphate to glycerol and from glucose-6-phosphate to the pentose phosphate pathway, which were found to reduce ethanol production and maintain the metabolic balance. Flux distribution analysis also revealed connections among organic acids, amino acids, and fermentation products, highlighting the role of a partial TCA cycle during fermentation. Additionally, metabolic interactions between SC and HU were identified, contributing to the enhanced production of volatile compounds, such as 2-phenylethanol and indole-3-ethanol in mixed fermentation. These findings provide a more comprehensive understanding of transcriptional regulation and metabolic strategies under fermentation conditions. They also offer practical targets for future bioengineering efforts aimed at controlling and optimizing the wine flavor.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"96 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bicycled Hairpin Assembly-Mediated SERS/Colorimetric Dual-Mode Sensor for the Detection of Dexamethasone in Milk and Pork","authors":"Rui Tan, Ziji OuBu, Qun Zhou, Juan Mu, Chenxi Yi, Xiaojun Luo","doi":"10.1021/acs.jafc.5c03268","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03268","url":null,"abstract":"The presence of dexamethasone (DEX) residues and the illegal addition of DEX to food have garnered widespread public concern over recent decades. Based on the bicycled hairpin assembly (BHA) mechanism and the catalytic oxidase effect of urchin-shaped Au–Ag alloy loaded with Pt nanoparticles (Au@Ag@Pt), an accurate and ultrasensitive surface-enhanced Raman scattering (SERS)/colorimetric dual-mode method for DEX detection is pioneered. When DEX is introduced into the sensor system, the BHA cycle begins. The Fe₃O₄ nanosphere loaded with Au nanoparticles (Fe₃O₄@Au) surface facilitates the anchoring of a large amount of Au@Ag@Pt, resulting in synergistic signal amplification and facile magnetic separation from the complex system. Finally, the collected product effectively catalyzes the conversion of 3,3′,5,5′-tetramethylbenzidine (TMB) to the oxidized product, producing a strong SERS response, an ultraviolet–visible absorption signal, and color deepening. Our dual-mode platform achieves ultrasensitive detection of DEX, achieving a detection limit (LOD) of 6.61 × 10^–17 M within the broad range of 10^–16 to 10^–9 M for SERS and a LOD of 8.81 × 10^–13 and 6.98 × 10^–13 M within the range of 10^–12–10^–5 M for the colorimetric method. Additionally, this platform shows high selectivity and satisfactory recovery rates for DEX in milk and pork samples, with recoveries of 92.3%–106.5% (RSDs &lt;8.35%) for SERS and 95.2%–107.6% (RSDs &lt;8.81%) for colorimetric analysis. This study demonstrates that by combining the BHA amplification process with multifunctional Au@Ag@Pt and Fe₃O₄@Au substrates, the DEX content in actual food samples can be accurately determined using a dual-mode approach that combines the high sensitivity of SERS with the simplicity of colorimetric analysis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymes, Proteins, and Peptides as Promising Biosolutions for Crop Production","authors":"Baptiste Kergaravat, Laure Plener, Magali Castan, Damien Grizard, Éric Chabrière, David Daudé","doi":"10.1021/acs.jafc.5c01107","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01107","url":null,"abstract":"The development of environmentally friendly alternatives to plant protection products is essential for sustainable agriculture. In this context, natural compounds, such as enzymes, proteins, and peptides, represent a promising reservoir of bioactive molecules. Although little represented on the market, their potential as fertilizers, biostimulants, and biopesticides is highlighted by numerous reports and patents, which the present review aims to bring together. While fertilization and biostimulation are primarily aimed at improving plant yield and health, biopesticides can be used to target a wide range of microbial plant pathogens and pests. The nature and effects of proteinaceous molecules are discussed in light of restrictive regulations.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Function of SfDNAJA1 and SfHSP68 in Temperature Stress Response and Apoptosis in Fall Armyworm (Spodoptera frugiperda)","authors":"Hao Yang, Sicheng Li, Bo Yu, Banghong Jian, Yong Duan, Chunyu Hu, Baoshan Chen, Yang Zhao","doi":"10.1021/acs.jafc.5c02002","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02002","url":null,"abstract":"The fall armyworm (<i>Spodoptera frugiperda</i>) is a major invasive pest. To explore its adaptive mechanisms under temperature stress, we conducted transcriptome analysis across six developmental stages and both sexes at 0, 26, and 46 °C. High-temperature stress induced more differentially expressed genes (DEGs, 8,703) than low-temperature stress (5,426), with fourth instar larvae showing the most DEGs at low temperatures. Sex-specific responses were also evident. Sixteen heat shock protein (HSP) genes and 31 apoptosis-related genes were identified as key stress-responsive factors. RNAi knockdown of <i>SfDNAJA1</i> and <i>SfHSP68</i> reduced survival under temperature stress, increased ROS and Cyt <i>c</i> levels, and upregulated apoptosis-related genes, while ATP levels decreased. Elevated caspase-3, G6PD, and GST activities further indicated oxidative and apoptotic responses. These results underscore the essential role of HSPs in maintaining cellular homeostasis and regulating apoptosis during thermal stress, offering insights into pest adaptation and potential control strategies.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"84 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of a Novel Alginate Lyase VSAly7C with Potential Application for Alginate Di- and Tri-Saccharide Preparation","authors":"Yi Li, Yihou Deng, Yingjie Li, Lushan Wang","doi":"10.1021/acs.jafc.5c00257","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00257","url":null,"abstract":"Brown algae are the largest-producing macroalgae, and alginate lyase plays a key role in the green degradation and high-value conversion of brown algae. This study characterized a novel alginate lyase, VSAly7C, from the marine bacterium <i>Vibrio</i> sp. 8–14, which belongs to the PL7_5 subfamily. Biochemical analysis suggested that VSAly7C is medium-temperature, neutral, and polyG-preferred, with enzyme activities of 2608.3 ± 27.3, 1453.2 ± 50.2, and 2545.2 ± 13.2 U/mg toward polyG, polyM, and sodium alginate, respectively. The minimal oligosaccharides VSAly7C could degrade were tetrasaccharides, and its major products were disaccharides and trisaccharides. Structural bioinformatic analysis of the VSAly7C active groove showed that the −1 to +3 subsite interaction network is crucial for determining the minimal oligosaccharides it can degrade. This study elucidates the catalytic properties, modes of action, and substrate recognition mechanisms of a novel alginate lyase, VSAly7C, which may be potentially applicable in alginate disaccharide and trisaccharide preparation.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"44 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Glucosinolate Sulfatases to Combat Plutella xylostella: Development of Novel Phenylcarboxamide Derivatives for Plant-Integrated Pest Management","authors":"Jiali Wang, Manwen Wang, Anil Ravi, Seyed-Omar Zaraei, Bilal O. Alkubaisi, Yingjie Wen, Lingda Zeng, Mohammed I. El-Gamal, Hanhong Xu","doi":"10.1021/acs.jafc.5c01873","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c01873","url":null,"abstract":"Glucosinolates in cruciferous plants are hydrolyzed by myrosinase to produce toxic isothiocyanates (ITCs). However, <i>Plutella xylostella</i> (<i>P. xylostella</i>), one of the top 10 global agricultural pests, utilizes glucosinolate sulfatases (GSSs) in its gut to convert glucosinolates into nontoxic desulfo-glucosinolates, thereby effectively avoiding the toxicity of ITCs. This study investigates the potential of inhibiting GSSs. Among 16 synthesized phenylcarboxamide derivatives, compound <b>1n</b> exhibited strong inhibitory activity against GSS1 (59.72%) and GSS2 (88.47%), key enzymes involved in glucosinolate desulfation in <i>P. xylostella</i>, leading to toxic ITC accumulation and disrupted detoxification. This resulted in an approximately 30% reduction in larval weight, at a concentration of 100 mg/L, the mortality rate reached 96%. Importantly, in the absence of glucosinolates, these inhibitors showed no direct toxicity to the insect, indicating that their action relies on interaction with the plant’s chemical defense system. These findings provide strong evidence supporting the development of GSSs inhibitions as more specific and selective insecticide, offering a promising alternative to conventional chemical pesticides.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"22 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, Synthesis, and Antibacterial Activity of Stilbene Derivatives against Phytopathogenic Bacteria","authors":"Peng Deng, Jun-Xia An, Hong-Jie Liang, Guo-Liang Mou, Xiong-Fei Luo, Deng-Tuo Wang, Shao-Yong Zhang, Li Jing Zhang, Ya-Rui Jin, Zhi-Jun Zhang, Ying-Qian Liu","doi":"10.1021/acs.jafc.5c02030","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02030","url":null,"abstract":"The increasing resistance of phytopathogenic bacteria to chemical pesticides has underscored the urgent need for novel bactericidal agents. In this study, we designed, synthesized, and evaluated a series of stilbene derivatives for their antibacterial activity against six strains of plant-pathogenic bacteria. Among them, compound <b>ST-A-8</b> showed the optimal activity against <i>Xanthomonas oryzae</i> pv <i>oryzae</i> (<i>Xoo</i>) and <i>Erwinia aroideae</i>, with both MIC values of 1.56 μg/mL, which was more potent than the commercialized antibacterial agent thiodiazole copper. Furthermore, the mechanism of <b>ST-A-8</b> on <i>Xoo</i> was preliminarily investigated, and the results showed that <b>ST-A-8</b> inhibited the biofilm formation as well as the production of extracellular polysaccharides in <i>Xoo</i>. Additionally, it disrupted the integrity of the cell membrane and increased the production of ROS, leading to lipid peroxidation of the cell membrane. Therefore, compound <b>ST-A-8</b> may serve as a potential novel bactericide for the control of plant bacterial diseases.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"12 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of Sennidin B as a Potent Multitarget Inhibitor of Insect Chitinolytic Enzymes","authors":"Zihan Pang, Huijie Xie, Xi Jiang, Yuhang He, Jinhui Liang, Yi Ding, Tian Liu","doi":"10.1021/acs.jafc.5c02612","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02612","url":null,"abstract":"Natural products with multitarget inhibitory activity against insect chitinolytic enzymes offer the potential for developing environmentally friendly insecticides. However, most inhibitors show limited efficacy, hindering their practical application. In this study, inspired by the dimeric structure of phlegmacin B₁, sennidin B, a rhein analogue, was identified as an effective inhibitor of insect chitinolytic enzymes. Sennidin B exhibited a nanomolar <i>K</i>_i value (80 nM) against <i>Of</i>Chi-h from <i>Ostrinia furnacalis</i>, showing at least a 600-fold improvement in potency compared to rhein. Molecular dynamics simulations revealed that sennidin B adopts a folded conformation within the enzyme’s active site, enhancing binding affinity through π–π stacking interactions with a key tryptophan residue. Insecticidal assays demonstrated 100% mortality at 5 mM and suppression of larval development at 1 mM. This study positions sennidin B as a lead compound for the development of insecticides targeting the molting process and provides a strategy for the discovery of multitarget inhibitors.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsVQ32–OsWRKY53 Module Regulates Rice Resistance to Bacterial Blight by Suppressing OsPrx30-Mediated ROS Scavenging","authors":"Wenya Xie, Xinru Li, Xuanyu Yue, Shimin Zuo, Meng Yuan","doi":"10.1021/acs.jafc.4c12968","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12968","url":null,"abstract":"WRKY transcription factors play crucial roles in regulating plant immune responses. Our previous research showed that <i>OsWRKY53</i> negatively affects rice resistance to bacterial blight by reducing the thickness of the cell wall. In this study, we identified a physical interaction between the OsWRKY53 and the OsVQ32, revealing that the OsWRKY53 functions as a downstream component of the OsMPK4–OsVQ32 cascade. OsWRKY53 can directly bind to the promoter of <i>OsPrx30</i>, a gene that negatively affects rice resistance to bacterial blight, thereby activating its expression. <i>OsWRKY53</i>-overexpressing plants exhibited a significant increase in peroxidase activity and a decrease in hydrogen peroxide content, whereas the opposite effects were observed in the <i>oswrky53</i> mutants. Furthermore, we found that the interaction between OsVQ32 and OsWRKY53 forms a complex that represses <i>OsPrx30</i> transcription by enhancing the DNA-binding activity of OsWRKY53. OsVQ32 phosphorylation by the OsMPK4 further enhanced this suppression. In conclusion, our findings suggest that the OsVQ32–OsWRKY53 complex regulates the expression of <i>OsPrx30</i>, modulating resistance to bacterial blight by suppressing the scavenging of reactive oxygen species.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"27 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"9β-Hydroxy-Ageraphoron Enhances TMV Resistance in Tobacco by Activating the SA Signaling Pathway through Upregulation of MYB and DOF Genes","authors":"Yang Liu, Taijiao Yang, Yanyi He, Anjian Zuo, Qing Li, Shanshan Nie, Shijun Hu, Xiaohui Yan","doi":"10.1021/acs.jafc.4c11533","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11533","url":null,"abstract":"Tobacco mosaic virus (TMV) poses a significant threat to global tobacco production. In this study, nine sesquiterpenoids isolated from <i>Ageratina adenophora</i> were evaluated for their anti-TMV activity using the half-leaf method. Among them, compound <b>1</b> (9β-hydroxy-ageraphorone) demonstrated the highest therapeutic (42.97%) and superior inactivation (48.87%) efficacy at 50 μg/mL. Further studies revealed that compound <b>1</b> disrupted TMV particle integrity and accelerated virion polymerization via hydrogen bonding and hydrophobic interactions with the viral coat protein. Additionally, it upregulated salicylic acid (SA) levels and enhanced the activities of PAL, POD, and SOD. Transcriptome and qRT-PCR analyses further indicated that compound <b>1</b> upregulated stress-responsive genes particularly <i>MYB</i> and <i>LBD</i> and activated the SA signaling pathway, leading to elevated expression of disease-resistant transcription factors and proteins, thereby enhancing TMV resistance in tobacco. These findings highlight 9β-hydroxy-ageraphorone as a promising candidate for botanical antiviral agents, contributing to the development of eco-friendly crop protection strategies.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"34 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}