Journal of Agricultural and Food Chemistry最新文献_第2页

Methoxyfenozide and Lufenuron Enhanced Insecticidal Activity via 20E Biosynthesis-Immunity Dual Disruption in Spodoptera exigua (Lepidoptera, Noctuidae). 甲氧虫酰肼和氟虫腈通过20E生物合成-免疫双重干扰增强对夜蛾夜蛾的杀虫活性。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c10854

Zhixian Zhang,Dan Wang,Yajie Ma,Yongpan Shan,Xianpeng Song,Hongyan Hu,Changcai Wu,Yan Ma,Xiangliang Ren

{"title":"Methoxyfenozide and Lufenuron Enhanced Insecticidal Activity via 20E Biosynthesis-Immunity Dual Disruption in Spodoptera exigua (Lepidoptera, Noctuidae).","authors":"Zhixian Zhang,Dan Wang,Yajie Ma,Yongpan Shan,Xianpeng Song,Hongyan Hu,Changcai Wu,Yan Ma,Xiangliang Ren","doi":"10.1021/acs.jafc.5c10854","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c10854","url":null,"abstract":"Spodoptera exigua (Lepidoptera, Noctuidae) poses significant threats to global agriculture, necessitating the management of resistance through sustainable alternatives such as Insect growth regulators (IGRs), including Methoxyfenozide (MET) and Lufenuron (LUF). This study elucidates the potential regulatory mechanisms between 20-hydroxyecdysone (20E) and immune signaling under IGR exposure. Sublethal doses of MET, LUF, and their mixture (MML) significantly downregulated the 20E titer by upregulating SeShd and SeCYP18A1. Silencing SeShd decreased 20E, whereas knockdown SeCYP18A1 increased 20E; both delayed development and increased susceptibility to IGRs. Furthermore, IGRs activated immune pathways (Toll/IMD), elevating SeDif and SePGRP-LB (SePLB) expression while simultaneously suppressing 20E. Silencing SeDif and SePLB stimulated 20E biosynthesis and heightened susceptibility to IGRs. These findings offer novel insights into the intricate interplay between 20E biosynthesis and the immune response to IGRs. Our work enhances the understanding of the IGRs' molecular mechanism and offers actionable strategies for combating resistance in S. exigua.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"3 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Tartaric Acid/Sodium Tartrate Buffer System for Xylo-Oligosaccharide Production from Moso Bamboo. 一种新型酒石酸/酒石酸钠缓冲体系用于毛竹生产低聚木糖。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c08783

Juan Wang,Wenjun Ying,Peiyao Wen,Hong Liao,Junhua Zhang

引用次数: 0

Improving the Catalytic Activity of a Quinone-Dependent Dehydrogenase for Deoxynivalenol Detoxification by Engineering the Conformation of the N-Terminal Loop. 通过改造n端环的构象来提高醌依赖脱氢酶对脱氧雪腐烯醇解毒的催化活性。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c07147

Yufeng Liu,Wenliang Hao,Yabo Liu,Jingyuan Si,Yuanyi Chen,Jing Li,Wenge Zheng,Jiantao Jiao,Ting Xie,Pingping Zhou,Chunmei Pan

{"title":"Improving the Catalytic Activity of a Quinone-Dependent Dehydrogenase for Deoxynivalenol Detoxification by Engineering the Conformation of the N-Terminal Loop.","authors":"Yufeng Liu,Wenliang Hao,Yabo Liu,Jingyuan Si,Yuanyi Chen,Jing Li,Wenge Zheng,Jiantao Jiao,Ting Xie,Pingping Zhou,Chunmei Pan","doi":"10.1021/acs.jafc.5c07147","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07147","url":null,"abstract":"The low oxidation activity of pyrroloquinoline quinone (PQQ)-dependent dehydrogenase (DepA) toward deoxynivalenol (DON) restricts its practical application in the food industry. Here, bioinformatics analysis indicated that the N-terminal loop of DepA acts as a lid that swings above the active pocket and plays an important role in controlling substrate entry. Considering that different PQQ-dependent dehydrogenases have different N-terminal loop lengths, we constructed DepA N-terminal truncation mutants. The catalytic efficiency (kcat/Km) of the best truncation mutant, N21, increased by 7.9-fold. Computational analysis suggested that mutant N21 maintains the open conformation of the N-terminal loop and widens the substrate channel to increase the level of substrate entry. In addition, the active pocket of mutant N21 was reshaped, which strengthened the interaction between DON and the active cavity residues and reduced the proton transfer distance, enhancing catalytic activity. This study provides new insights into engineering the catalytic activity of PQQ-dependent dehydrogenases.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"94 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Screening of an Anti-interference Histamine Biosensor Based on Modulation-Driven Arrays on a Chip. 基于芯片调制驱动阵列的抗干扰组胺生物传感器的快速筛选。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c08561

Wei-Cheng Lin,Chun-Ting Hsieh,Yun-Yu Hsieh,Wei-Lun Yen

引用次数: 0

In Vivo Investigation on the Effect of Gut Microbiota on the Distribution and Biotransformation of Curcumin. 肠道菌群对姜黄素分布和生物转化影响的体内研究。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c09992

Fudi Luo,Jing Chen,Shaojie Liang,Xiaohui Wang,Xingxing Chai,Ziyang Zheng,Dajian Zhu,Lianxian Guo

{"title":"In Vivo Investigation on the Effect of Gut Microbiota on the Distribution and Biotransformation of Curcumin.","authors":"Fudi Luo,Jing Chen,Shaojie Liang,Xiaohui Wang,Xingxing Chai,Ziyang Zheng,Dajian Zhu,Lianxian Guo","doi":"10.1021/acs.jafc.5c09992","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09992","url":null,"abstract":"Curcumin indicates varying sensitivities among individuals. To explore the influence of gut microbiota on the bioavailability and biotransformation of curcumin, a gut microbiota clearance model was established by using ceftriaxone. A total of 20 metabolites were identified and quantified by UPLC-MS/MS. The results indicated that (1) curcumin was predominantly distributed in the gastrointestine, and its distribution in the colon showed a significant positive correlation with the kidney; (2) the disrupted gut microbiota increased the excretion of curcumin in urine and inhibited the biotransformation of bioactive metabolites, such as tetrahydrocurcumin and hexahydrocurcumin; (3) the differences in curcumin metabolism were associated with changes in gut microbiota; (4) an in vitro experiment showed that gut microbiota can enhance the transformation of bioactive metabolites. Therefore, gut microbiota may influence the bioavailability and biotransformation of curcumin. This study enhances our understanding of the metabolism of curcumin by the gut microbiota.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"36 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics and Postbiotics Produced by Lacticaseibacillus rhamnosus 1.0320 Isolated from Traditional Fermented Dairy Products Alleviate DSS-Induced Colitis by Modulating the Gut Microbiota and Intestinal Metabolism. 传统发酵乳制品中鼠李糖乳杆菌1.0320产生的益生菌和后益生菌通过调节肠道菌群和肠道代谢缓解dss诱导的结肠炎

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c04623

Jing Zhang,Zhongmei Tan,Zihao Tian,Mingzhang Zhao,Shanshan Qian,Hongxia Feng,Yunhe Chang,Juncai Hou,Zhanmei Jiang

{"title":"Probiotics and Postbiotics Produced by Lacticaseibacillus rhamnosus 1.0320 Isolated from Traditional Fermented Dairy Products Alleviate DSS-Induced Colitis by Modulating the Gut Microbiota and Intestinal Metabolism.","authors":"Jing Zhang,Zhongmei Tan,Zihao Tian,Mingzhang Zhao,Shanshan Qian,Hongxia Feng,Yunhe Chang,Juncai Hou,Zhanmei Jiang","doi":"10.1021/acs.jafc.5c04623","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04623","url":null,"abstract":"Postbiotics are emerging as promising alternatives to probiotics. However, the mechanisms underlying their regulation of the gut microbiota-metabolite axis, signaling pathways, and multiomics interactions, as well as the key bioactive components responsible for their efficacy variations, remain poorly characterized. This study investigated the protective effects of Lactobacillus rhamnosus 1.0320 (probiotic, 1.0320) and heat-killed Lactobacillus rhamnosus 1.0320 (postbiotic, HK 1.0320) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). Results demonstrated that HK1.0320 significantly alleviated UC symptoms, mitigated colonic damage, restored the intestinal barrier, attenuated oxidative stress, regulated MPO activity, suppressed TLR4/MAPK/NF-κB pathway key proteins (TLR4, MyD88, JNK, p38, and p65), decreased pro-inflammatory cytokine secretion, modulated gut microbiota, and enhanced SCFA production. Untargeted metabolomics revealed that HK1.0320 is rich in bioactive components such as carboxylic acids and alleviates UC through its own or intestinal metabolic modification. This study clarifies the postbiotic colitis-relief mechanism and highlights their potential in functional foods or precision nutrition.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"98 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Triple-Focus Workflow of a Novel Nitrilase for Simultaneously Optimizing Activity, Thermostability, and Specificity. 同时优化活性、热稳定性和特异性的一种新型腈酶的三焦点工作流程。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c07983

Xiao-Ting Zhou,Ke-Yan Wei,Shan Li,Qi Shen,Ya-Ping Xue,Yu-Guo Zheng

{"title":"A Triple-Focus Workflow of a Novel Nitrilase for Simultaneously Optimizing Activity, Thermostability, and Specificity.","authors":"Xiao-Ting Zhou,Ke-Yan Wei,Shan Li,Qi Shen,Ya-Ping Xue,Yu-Guo Zheng","doi":"10.1021/acs.jafc.5c07983","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c07983","url":null,"abstract":"Nitrilases provide a sustainable route for directly converting nitriles to carboxylic acids. However, efficient biocatalytic synthesis of the agrochemical intermediate 3,6-dichloropicolinic acid(3,6-DCPA) from 3,6-dichloropicolinonitrile (3,6-DCPN) remains unreported. We identified a novel nitrilase from Rhodococcus pyridinivorans (RpNIT) and developed the integrated Feature Cluster-Multi-Force field-Evolutionary Co-Localization (FMEC) strategy to simultaneously enhance activity, thermostability, and specificity. FMEC employs hierarchical clustering, molecular dynamics simulations, and multiforce field algorithms, identifying 19 candidate residues. Using Parallel Iterative Saturation Mutagenesis, we generated the quintuple mutant M5a-DGPAT. This variant exhibited a 17.2 °C increase in melting temperature and a 3.3 × 104-fold improvement in hydrolytic efficiency (kcat/Km) over wild-type RpNIT, while abolishing hydratase activity. Structural analysis attributed the superior performance to an optimized rigidity-flexibility balance, refined substrate binding pocket geometry, and strengthened interaction networks. M5a-DGPAT achieved complete hydrolysis of 347.9 mM 3,6-DCPN to 3,6-DCPA under mild conditions, demonstrating significant industrial potential. This study establishes a robust computational framework for designing highly active, stable, and specific engineered enzymes.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"29 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Yield Production of Novel C30 Carotenoids with an Artificial Biosynthesis Pathway in Yarrowia lipolytica. 利用人工合成途径高产产新型类胡萝卜素C30

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c06970

Kun Zhu,Yonghao Li,Chengjiao Cao,Xiangyu Zhu,Shuli Liang,Xinying Zhang,Ying Lin

{"title":"High-Yield Production of Novel C30 Carotenoids with an Artificial Biosynthesis Pathway in Yarrowia lipolytica.","authors":"Kun Zhu,Yonghao Li,Chengjiao Cao,Xiangyu Zhu,Shuli Liang,Xinying Zhang,Ying Lin","doi":"10.1021/acs.jafc.5c06970","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c06970","url":null,"abstract":"Traditional microbial research has focused on C40 carotenoids. Recent reports showed that novel C30 carotenoid compounds, found in certain bacterial microorganisms, exhibited antioxidant properties similar to or greater than those of their C40 counterparts. However, the biosynthesis yields were relatively low. In this study, an artificial 8-step pathway for de novo mevalonate biosynthesis in Yarrowia lipolytica was constructed, increasing squalene titer to 1235.79 mg/L. This led to the development of a high-production squalene platform strain capable of supplying sufficient precursors for C30 carotenoid biosynthesis. We identified the low substrate conversion rate as the main limitation through a systematic research. The bottleneck was addressed through protein and compartmentalization engineering, resulting in 90.948 mg/L C30 carotenoid production (11.091 mg/g DCW). Ultimately, by incorporating these approaches, we developed strains capable of producing 2686.77 mg/L C30 carotenoids with a cellular content of 35.05 mg/g DCW in bioreactor fermentations, a significant improvement over the initial strain and the highest ever reported for C30 carotenoids.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive Regulatory Mechanisms of Armillaria mellea in Bamboo Media: A Biochemical Analysis. 蜜环菌在竹培养基中的适应性调控机制：生化分析。

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c09249

Qi Li,Jin Chen,Chun Shi,Dawei Wang,Jing Yang,Haiyan Yang,Zhengjun Shi

{"title":"Adaptive Regulatory Mechanisms of Armillaria mellea in Bamboo Media: A Biochemical Analysis.","authors":"Qi Li,Jin Chen,Chun Shi,Dawei Wang,Jing Yang,Haiyan Yang,Zhengjun Shi","doi":"10.1021/acs.jafc.5c09249","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c09249","url":null,"abstract":"Conventional wood-based substrates for cultivating Armillaria mellea pose ecological and economic challenges. Bamboo, a fast-growing renewable resource, offers a promising alternative. This study evaluated A. mellea's adaptability to bamboo media through biochemical analyses, including growth kinetics, extracellular enzyme activity, bioactive compound production, and the molecular degradation of cellulose, hemicellulose, and lignin. The results showed that bamboo substrates effectively supported A. mellea growth, with high cellulase (29.97 U/L) and xylanase (54.54 U/L) activities. Bioactive compounds accumulated significantly: protein (18.2 mg/g), polysaccharides (62.9 mg/mL), and triterpenes (82.9 mg/mL). After 40 days, the degradation rates were 66.2% for cellulose, 56.1% for hemicellulose, and 52.5% for lignin. Structural analysis revealed a preference for degrading glucose, arabinose, xylose, and aromatic lignin units. These findings confirm that bamboo is a sustainable and efficient substrate for A. mellea cultivation, improving both growth and degradation, with potential applications in sustainable agriculture and companion cropping with Gastrodia elata.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Paraherquamide E as an Eco-Friendly Insecticide against Asian Citrus Psyllid from the Endophyte Aspergillus aculeatus GC-147-5 and Its Mode of Action. 内生真菌刺曲霉GC-147-5对柑橘木虱生态友好型杀虫剂的发现及其作用模式

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI: 10.1021/acs.jafc.5c06523

Mingnan You,Liangliang Gao,Yan Liang,Qin Xiong,Jun Zhang

{"title":"Discovery of Paraherquamide E as an Eco-Friendly Insecticide against Asian Citrus Psyllid from the Endophyte Aspergillus aculeatus GC-147-5 and Its Mode of Action.","authors":"Mingnan You,Liangliang Gao,Yan Liang,Qin Xiong,Jun Zhang","doi":"10.1021/acs.jafc.5c06523","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c06523","url":null,"abstract":"The Asian citrus psyllid (ACP) is the primary vector of huanglongbing (HLB), a severe citrus disease. This study identified three insecticidal paraherquamide alkaloids (paraherquamide E, SB200437, and paraherquamide B) that were effective against the ACP from the strain Aspergillus aculeatus GC-147-5 using a bioactivity-guided isolation method. The median lethal doses (LD50) of paraherquamide E were 19.93 μg/mL (24 h) and 29.08 μg/mL (12 h), comparable to those of imidacloprid. Investigations into the insecticidal mechanism revealed that paraherquamide E disrupts fatty acid synthesis, inhibits vitellogenin production, and alters metabolic homeostasis, ultimately leading to ACP mortality. Microscopic analysis confirmed ovarian abnormalities in treated females. Additionally, paraherquamide E exhibited negligible toxicity to the nontarget species, the ladybug, indicating its high selectivity and favorable environmental safety profile. The findings of this study could lead to the development of new, environmentally friendly, and effective insecticidal agents for managing ACP.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"62 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0