Geoffrey T. Broadhead, Hui Liu, Gretchen L. Sumrall, Anna K. Block, Charles T. Hunter, John J. Beck
{"title":"Volatile Byproducts of Carotenoid Degradation as Biomarkers of Maize Infestation by the Maize Weevil (Sitophilus zeamais) (Motsch.)","authors":"Geoffrey T. Broadhead, Hui Liu, Gretchen L. Sumrall, Anna K. Block, Charles T. Hunter, John J. Beck","doi":"10.1021/acs.jafc.4c09665","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09665","url":null,"abstract":"Maize (<i>Zea mays</i>) is a major global food crop and a source of industrial raw materials. Effective postharvest storage is important for national food security programs, international trade, and global agriculture economics. The maize weevil (<i>Sitophilus zeamais</i>) is a primary postharvest insect pest that infests maize during storage and leads to significant losses. Using multivariate discriminant analysis of volatile profiles collected from intact and infested maize, we identified two volatile apocarotenoids, 6-methyl-5-hepten-2-one and 6-methyl-5-hepten-2-ol, as indicators of maize weevil infestation in stored maize. Emission of these biomarker compounds rapidly signaled maize weevil infestation and showed a significant correlation with oviposition damage to the stored kernels. The pattern of elevated biomarker emission after weevil exposure was consistent across all seven maize lines examined. These volatile biomarkers can be used for early detection and removal of infested maize and can aid in the control of this pest.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"269 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tao Sun, Mei-Li Sun, Lu Lin, Jian Gao, Kaifeng Wang, Xiao-Jun Ji
{"title":"Advancing Succinic Acid Biomanufacturing Using the Nonconventional Yeast Yarrowia lipolytica","authors":"Tao Sun, Mei-Li Sun, Lu Lin, Jian Gao, Kaifeng Wang, Xiao-Jun Ji","doi":"10.1021/acs.jafc.4c09990","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09990","url":null,"abstract":"Succinic acid is an essential bulk chemical with wide-ranging applications in materials, food, and pharmaceuticals. With the advancement of biotechnology, there has been a surge in focus on low-carbon sustainable microbial synthesis methods for producing biobased succinic acid. Due to its high intrinsic acid tolerance, <i>Yarrowia lipolytica</i> has gained recognition as a competitive chassis for the industrial manufacture of succinic acid. This review summarizes the research progress on succinic acid biomanufacturing using <i>Y. lipolytica</i>. First, it introduces the major metabolic routes for succinic acid biosynthesis and the pertinent engineering approaches for building efficient cell factories. Subsequently, we offer a review of methods employed for succinic acid synthesis by <i>Y. lipolytica</i> utilizing alternative substrates as well as the relevant optimization strategies for the fermentation process. Finally, future research directions for improving succinic acid biomanufacturing in <i>Y. lipolytica</i> are delineated in light of the recent progress, obstacles, and trends in this area.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"19 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chengkun Li, Junmin Wang, Haijiao Dong, Dongchen Yang, Peng Li, Shuang Cao, Chao Li, Zexiu An, Jinlin Zhang, Yan-En Wang
{"title":"Design, Synthesis, and Herbicidal Activity Study of Novel Pyrazole-Carboxamides as Potential Transketolase Inhibitors","authors":"Chengkun Li, Junmin Wang, Haijiao Dong, Dongchen Yang, Peng Li, Shuang Cao, Chao Li, Zexiu An, Jinlin Zhang, Yan-En Wang","doi":"10.1021/acs.jafc.4c08397","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08397","url":null,"abstract":"Transketolase (TKL; EC 2.2.1.1) has been identified as a potential new herbicide target. In order to discover highly herbicidal active compounds targeting TKL and improve their structural diversity for lead compounds, a series of pyrazole-carboxamides <b>7a</b>–<b>7v</b> were designed and synthesized through structural optimization for pyrazole-containing phenoxy amide compound <b>4u</b>. Among the synthesized compounds, compound <b>7r</b> possessed excellent herbicidal efficacy against <i>Digitaria sanguinalis</i> (<i>Ds</i>) and <i>Amaranthus retroflexus</i> (<i>Ar</i>) by the small cup method (the inhibition about 95%, 100 mg/L) and the foliar spray method (the inhibition over 90%, 150 g ai/ha) in a greenhouse, which were superior to that of the positive control nicosulfuron. More significantly, compound <b>7r</b> displayed good crop selectivity toward both maize and wheat even at 375 g of ai/ha. The studies on mode of action (MOA) of high herbicidal active compounds, including the enzyme inhibition activity, fluorescent quenching experiments, and molecular docking analysis between <i>Setaria viridis</i> (<i>Sv</i>)TKL and ligand, suggested that compound <b>7r</b> acts as a typical TKL inhibitor, and the benzothiazole ring is an important motif for <i>Sv</i>TKL inhibition activity. Above all, compound <b>7r</b> could be a potential candidate for the development of herbicides with new MOA for weed control in maize and wheat field.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"82 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to “CF2–II Alternative Splicing Isoform Regulates the Expression of Xenobiotic Tolerance-Related Cytochrome P450 CYP6CY22 in Aphis gossypii Glover”","authors":"Yaping Ding, Jianyi Li, Kunpeng Yan, Long Jin, Chengcheng Fan, Rui Bi, Haoran Kong, Yiou Pan, Qingli Shang","doi":"10.1021/acs.jafc.4c11996","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11996","url":null,"abstract":"A corrected version of Figure 9 appears below. An incorrect image appeared in the negative control in the original version. No bacteria could grow in medium lacking Leu with ABA (600 ng/mL) in the negative control. This correction does not change the scientific conclusions of the article. Figure 9. Structure of the <i>CYP6CY22</i> promoter region. (A) Structure of the 5′-flanking region of <i>CYP6CY22</i>. The TSS in the figure represents the transcription start site. The transcription starting point number is +1, with the upstream sequence starting with “–” and the downstream sequence starting with “+”. The predicted <i>CF</i><sub>2</sub><i>–II-AS</i> protein binding sites and mutant sequence are indicated. (B) Investigation of the interaction between <i>CYP6CY22.p</i> and <i>CF</i><sub>2</sub><i>–II-AS</i> by the Y1H assay. Yeast transformants containing the <i>CYP6CY22.p</i> vector and TF plasmid were selected on SD/-Leu media supplemented with ABA. Positive control: yeast strain transformed with the pGADT7-<i>p53</i> and pAbAi-<i>p53</i> plasmids; negative control: yeast strain transformed with pAbAi-<i>CYP6CY22.p</i>. This article has not yet been cited by other publications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"77 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Jiao, Jian Ling, Raja Asad Ali Khan, Ning Luo, Zixin Li, Zeyu Li, Yuhong Yang, Jianlong Zhao, Zhenchuan Mao, Gerald F. Bills, Bingyan Xie, Yan Li
{"title":"Genome Mining Reveals Biosynthesis of the Antifungal Lipopeptaibols, Texenomycins, through a Hybrid PKS-NRPS System, in the Fungus Mariannaea elegans","authors":"Yang Jiao, Jian Ling, Raja Asad Ali Khan, Ning Luo, Zixin Li, Zeyu Li, Yuhong Yang, Jianlong Zhao, Zhenchuan Mao, Gerald F. Bills, Bingyan Xie, Yan Li","doi":"10.1021/acs.jafc.4c08847","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08847","url":null,"abstract":"Texenomycins are a family of linear lipopeptaibols with a long polyketide side chain at the N-terminus and 21 amino acid residues at the <i>C</i>-terminus, presenting demonstrated potential as antibiotics against plant fungal pathogens. In this study, texenomycins were identified and isolated from the fungus <i>Mariannaea elegans</i> strain TTI-0396 and showed effective antifungal properties against two plant pathogens <i>Colletotrichum lagenarium</i> and <i>Botrytis cinerea</i>. Through analysis of the whole-genome data of <i>M. elegans</i> strain TTI-0396, we discovered a hybrid PKS-NRPS system with the polyketide synthase (PKS: TexQ), thioesterase (TexO), acyl-CoA ligase (TexI), and three nonribosomal peptide synthetases (NRPSs: TexG, TexJ, TexV) in the <i>tex</i> gene cluster that were proposed to be responsible for the biosynthesis of texenomycins and another related lipopeptaibol, lipohexin. The functions of six key genes (<i>texQ</i>, <i>texO</i>, <i>texI</i>, <i>texG, texJ</i>, and <i>texV</i>) in the hybrid PKS-NRPS system were verified by gene deletion experiments, and five genes (<i>texQ</i>, <i>texO</i>, <i>texI</i>, <i>texG</i>, and <i>texV</i>) were confirmed to be responsible for the biosynthesis of texenomycins, while four genes (<i>texQ</i>, <i>texO</i>, <i>texI</i>, and <i>texJ</i>) were involved in the biosynthesis of lipohexin. Furthermore, the function of one transcription factor gene (<i>texR</i>), which enhanced the production of texenomycins by regulating the key genes in the <i>tex</i> gene cluster, was also demonstrated through gene deletion and overexpression experiments. Finally, a hypothetical scheme for texenomycins and lipohexin biosynthesis assembly is proposed. The elucidation of this intricate hybrid PKS-NRPS system has significantly deepened our comprehension of the mechanisms underlying the generation and chemical diversity of fungal lipopeptaibol natural products, offering a promising avenue for future research and potential applications in fungicidal disease control in agriculture.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"11 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemoproteomic Profiling Reveals Chlorogenic Acid as a Covalent Inhibitor of Arabidopsis Dehydroascorbate Reductase 1","authors":"Jingyuan Xu, Lijun Chen, Shanshan Wang, Wen Zhang, Jianjia Liang, Lu Ran, Zhangshuang Deng, Yiqing Zhou","doi":"10.1021/acs.jafc.4c07955","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c07955","url":null,"abstract":"Chlorogenic acid (CA) is an abundant plant secondary metabolite with promising allelopathic effects on weed growth. However, the molecular targets and mechanism of action of CA in plants remain elusive. Here, we report the employment of a clickable photoaffinity probe in identifying the protein targets of CA in <i>Arabidopsis</i> seedling proteomes. CA specifically binds <i>Arabidopsis</i> dehydroascorbate reductase 1 (<i>At</i>DHAR1), an enzyme responsible for ascorbate regeneration in plants, by covalent alkylating Cys20 within the catalytic center, thereby inhibiting its activity. In vivo application of CA reduced the pool size and redox state of ascorbate, leading to H<sub>2</sub>O<sub>2</sub> accumulation in <i>Arabidopsis</i> seedlings. In agreement with these results, CA significantly induced the upregulation of antioxidant enzymes and downregulation of proteins involved in water transport and photosynthesis, as evidenced by quantitative proteomics. Taken together, this study revealed DHAR1 as a functional target underlying CA’s allelopathic activity in plants, which opens new opportunities for the development of novel herbicides from naturally existing resources.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"70 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Encapsulation and Sustained Release of Quercetin-Loaded pH-Responsive Intelligent Nanovehicles Based on the Coassembly of Pea Protein Isolate and Hyaluronic Acid","authors":"Xingnan Wang, Yang Yang, Yanting Chen, Ting Liu, Jingyi Ren, Hongcai Li, Wenzhi Lei, Shiqi Li, Zhenpeng Gao","doi":"10.1021/acs.jafc.4c08659","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c08659","url":null,"abstract":"A pea protein isolate (PPI)-hyaluronic acid (HA) nanocarrier delivery system was created for quercetin (Que) encapsulation using the pH conversion strategy. The self-assembly of the PPI-HA binary nanocomplex (HPP) were mainly driven by electrostatic and hydrophobic interactions. Que was successfully encapsulated in HPP nanocomposites (Que@HPP), which exhibited preferable redispersibility, and encapsulation efficiency (87.51%), loading capacity (14.50%). Que@HPP provided superior resistance to external environmental stresses (pH, ionic strength, high temperature, light exposure, and long-term storage), while maintaining its primary antioxidant activity after 15 days. Compared to free Que, the encapsulated Que shifted from a crystalline to an amorphous form, diffusing more easily through nanoparticle pores. Moreover, the encapsulated Que (Que@HPP) were stable in simulated gastric fluid (SGF, pH = 1.2) and released slowly in simulated intestinal fluid (SIF, pH = 6.8) compared to naked Que, demonstrating its potential to respond to specific external pH environments. Thus, the fabrication of HPP nanovehicles for Que encapsulation is a viable solution to improve its stability and release behaviors.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"51 4 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lei Feng, Mingguang Ju, Chunyan Ma, Kai Li, Shuang Cai
{"title":"Immunomodulatory Acidic Polysaccharide from Jujube Fruit (Zizyphus jujuba Mill.): Insight into Their Chemical Characteristics and Modes of Action","authors":"Lei Feng, Mingguang Ju, Chunyan Ma, Kai Li, Shuang Cai","doi":"10.1021/acs.jafc.4c06905","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c06905","url":null,"abstract":"Jujube (<i>Zizyphus jujuba</i> Mill.) has been consumed globally as a fruit and a nutraceutical food for millennia. This study presents the isolation and purification of a novel water-soluble polysaccharide fraction, ZJMP-2, from <i>Z. jujuba</i> Mill. ZJMP-2 underwent characterization through Fourier transform infrared (FT-IR), high-performance gel permeation chromatography-laser light scattering (HPGPC-LLS), gas chromatography-mass spectrometry (GC-MS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and nuclear magnetic resonance (NMR) analyses. It consists of glucose, galactose, arabinose, rhamnose, and galacturonic acid in molar ratios of 0.41:0.08:0.11:0.05:0.33 and boasts an average molecular weight of approximately 57.8 kDa. Its backbone features the structure →2)-α-<span>l</span>-Rha<i>p</i>-(1 → 4)-α-<span>d</span>-Gal<i>p</i>A-(1 → 4)-α-<span>d</span>-Gal<i>p</i>A-6OMe-(1 → 4)-α-<span>d</span>-Gal<i>p</i>A-(1 → 3, 4)-α-<span>d</span>-Glc<i>p</i>-(1 →, with branching at →5)-α-<span>l</span>-Ara<i>f</i>-(1 →, →4)-β-<span>d</span>-Gal<i>p</i>-(1 →, and →4)-α-<span>d</span>-Glc<i>p</i>-(1→ at position O-3 of →3, 4)-α-<span>d</span>-Glc<i>p</i>-(1 →. These structural variations contribute to the pronounced immunoregulatory effects of ZJMP-2. Specifically, ZJMP-2 significantly elevated the expression levels of TLR4, NF-κB, and TRAF6 proteins, enhancing RAW264.7 cell activity, index of splenic lymphocytes, and the production of cytokines and NO, thereby activating macrophages and promoting lymphocyte proliferation. <i>In vivo</i> studies demonstrated that ZJMP-2 promoted the heart, spleen, and bone marrow indices, peripheral blood and spleen cell counts, and the number of heart and bone marrow cells in mice. These findings demonstrated that ZJMP-2 has potential as an immunomodulator and provides valuable insights for developing natural immunomodulators in the pharmaceutical industry.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying-Xin Zhao, Xue-Nan Li, Yi-Xi Tang, Milton Talukder, Yi Zhao, Jin-Long Li
{"title":"Correction to “Cadmium Transforms Astrocytes into the A1 Subtype via Inducing Gap Junction Protein Connexin 43 into the Nucleus”","authors":"Ying-Xin Zhao, Xue-Nan Li, Yi-Xi Tang, Milton Talukder, Yi Zhao, Jin-Long Li","doi":"10.1021/acs.jafc.4c12209","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c12209","url":null,"abstract":"The following corrections of the affiliations of the authors are being made. Yi Zhao, College of Veterinary Medicine and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, P. R. China; phone, +86 451 55190407; email, zhaoyi@neau.edu.cn. Jin-Long Li, College of Veterinary Medicine and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, P. R. China; phone, +86 451 55190407; email, jinlongli@neau.edu.cn. Ying-Xin Zhao, College of Veterinary Medicine and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, P. R. China. Xue-Nan Li, College of Veterinary Medicine and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, P. R. China. Yi-Xi Tang, College of Veterinary Medicine and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin 150030, P. R. China. This article has not yet been cited by other publications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"54 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Naphpyrones A–H, Antibacterial Aromatic Polyketides Isolated from the Streptomyces coelicolor A3(2)/spi1 ΔspiH3","authors":"Xiaofei Huang, Xiao Xu, Luning Zhou, Chuanteng Ma, Wenxue Wang, Chen Li, Jiaxiang Wang, Guojian Zhang, Dehai Li, Qian Che, Tianjiao Zhu","doi":"10.1021/acs.jafc.4c09101","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c09101","url":null,"abstract":"<i>Staphylococcus aureus</i>, a common foodborne pathogen, has a close association with agriculture and food. With the rapid emergence and widespread dissemination of antimicrobial resistance, efforts have been directed toward developing and studying new antimicrobial compounds to inhibit the growth of <i>S. aureus</i> and other foodborne pathogens, thereby preventing contamination and ensuring food safety. Herein, we reported eight new aromatic polyketides, naphpyrones A–H (<b>1</b>–<b>8</b>), from the heterologous expression strain <i>Streptomyces coelicolor</i> A3(2)/<i>spi1</i> Δ<i>spi</i>H3. Their structures and absolute configurations were elucidated by extensive NMR, MS, theoretical NMR calculations, DP4+ probability analysis, Mosher’s method, and ECD analyses. Notably, naphpyrone A (<b>1</b>) featured an unprecedented 6/6/6/6/5 neocyclic skeleton. Bioactivity evaluation revealed that compounds <b>1</b> and <b>2</b> exhibited antibacterial activity against <i>S. aureus</i>, with MIC values of 1 μg/mL and 4 μg/mL, respectively. These findings highlight the potential for screening and developing therapeutic agents from <i>actinomycetes</i>-derived aromatic polyketides against food pathogens.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"29 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}