Journal of Agricultural and Food Chemistry最新文献_第2页

Sea Cucumber Fucoidan Inhibits Helicobacter pylori Gastritis via MAPK/NF-κB Signaling and Gut Microbiota Modulation 海参岩藻聚糖通过MAPK/NF-κB信号传导和肠道菌群调节抑制幽门螺杆菌胃炎

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-30 DOI: 10.1021/acs.jafc.5c02190

Junhan Cao, Mengke Yao, Kai Wang, Ling Qin, Qing Zhang, Haibo Zhang, Jinzheng Wei, Changfeng Qu, Changhu Xue, Jinlai Miao

{"title":"Sea Cucumber Fucoidan Inhibits Helicobacter pylori Gastritis via MAPK/NF-κB Signaling and Gut Microbiota Modulation","authors":"Junhan Cao, Mengke Yao, Kai Wang, Ling Qin, Qing Zhang, Haibo Zhang, Jinzheng Wei, Changfeng Qu, Changhu Xue, Jinlai Miao","doi":"10.1021/acs.jafc.5c02190","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02190","url":null,"abstract":"Antibiotic-based eradication therapy forHelicobacter pylori faces challenges such as the increasing number of antibiotic-resistant strains and gut microbiota dysbiosis. The study evaluated the effects of fucoidan from sea cucumber cooking liquid (Fuc-SC) on Helicobacter pylori Sydney strain SS1 (Hp SS1)-associated gastritis. The backbone structure of Fuc-SC was composed of alternating → 3)-α-L-Fucp-(1 → 4)-α-L-Fucp-(1 → linked fucose residues. The mouse experiments demonstrated that Fuc-SC reduced the colonization of Hp SS1 in the gastric mucosa through decreasing the level of urease, Hp-IgG, and CagA-IgG. In addition, Fuc-SC regulated oxidative stress levels by reducing NO, MDA, and ROS levels, while increasing the activity of SOD and GSH-Px. Compared to antibiotic treatment alone, the combination of Fuc-SC with antibiotics further enhanced the antioxidant effects. Molecular mechanisms indicated that Fuc-SC regulated the MAPK/NF-κB signaling pathway, downregulating the expression of inflammatory factors such as IL-1β and TNF-α and further modulating the expression of S100A8 and E-cadherin. Gut microbiota analysis indicated that Fuc-SC reversed the depletion of Lactobacillus caused by antibiotic treatment. Additionally, Fuc-SC significantly promoted the biosynthesis of microbial metabolites such as butyric acid, isobutyric acid, hexanoic acid, and phospholipids, thereby alleviating inflammation. These findings highlight its potential as a sustainable, nonantibiotic therapy for Helicobacter pylori-associated gastritis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"43 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Efficient and Economical One-Pot Two-Step Multienzymatic Synthesis of 6′/3′-Sialyllactosamine from In Situ-Produced N-Acetyllactosamine n -乙酰乳胺一锅两步多酶法高效经济合成6′/3′-唾液基乳胺

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c00395

Runmiao Jiao, Xinlv Peng, Bing Wang, Yuguang Du, Eika W. Qian, Jianjun Li

{"title":"Highly Efficient and Economical One-Pot Two-Step Multienzymatic Synthesis of 6′/3′-Sialyllactosamine from In Situ-Produced N-Acetyllactosamine","authors":"Runmiao Jiao, Xinlv Peng, Bing Wang, Yuguang Du, Eika W. Qian, Jianjun Li","doi":"10.1021/acs.jafc.5c00395","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00395","url":null,"abstract":"Among over 200 human milk oligosaccharides, 6′-SLN (6′-sialyl-N-acetyllactosamine) and 3′-SLN possess potential antiviral function and can potentially enhance brain development and knowledge. So far, no efficient and cost-effective synthetic pathways for 6′-SLN and 3′-SLN have been developed. In this study, efficient enzymatic pathways to synthesize 6′-SLN and 3′-SLN were established involving economic cytidine 5′-triphosphate (CTP) regeneration from commercial N-acetyllactosamine (LacNAc) with yields of >95%. To meet the requirement of industrial production, their synthesis was further optimized starting from in situ-produced LacNAc. Consequently, the cost of LacNAc purification could be saved. Furthermore, conducting synthesis at pH 7.0 and with supplementation of polyP6 in three batches led to yields of 6′-SLN (95.0%) and 3′-SLN (94.8%), respectively. Under optimized conditions, enzymatic syntheses of 6′-SLN and 3′-SLN were conducted at a 5 L scale with yields of 96.1% (259.2 g) and 92.3% (248.7 g), respectively. This study would lay the foundation for industrial production of 3′-SLN and 6′-SLN in the future and provide a guide for multienzymatic preparation of other sialylated oligosaccharides.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"16 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Allatostatin Type-A Receptor as a Promising Target for Discovering the Peptide Mimic A15 as an IGR Candidate with a Broader Insecticidal Spectrum 揭示Allatostatin - a型受体作为一个有希望的靶点，发现肽模拟A15作为具有更广泛杀虫谱的IGR候选物

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c02547

Yimeng Zhang, Yan Liu, Chen Chen, Wenyu Sun, Zhaokai Yang, Chandni Iqbal, Xinyuan Zhang, Yun Ling, Li Zhang, Xinling Yang

{"title":"Unveiling the Allatostatin Type-A Receptor as a Promising Target for Discovering the Peptide Mimic A15 as an IGR Candidate with a Broader Insecticidal Spectrum","authors":"Yimeng Zhang, Yan Liu, Chen Chen, Wenyu Sun, Zhaokai Yang, Chandni Iqbal, Xinyuan Zhang, Yun Ling, Li Zhang, Xinling Yang","doi":"10.1021/acs.jafc.5c02547","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02547","url":null,"abstract":"Allatostatin type-A receptor (AstR-A), a key regulator in insect physiology but not vertebrates, emerges as a promising target. In previous works, allatostatin (AST) analogues Q6 exhibited potent juvenile-hormone (JH) inhibitory activity against Diploptera punctata and unexpected insecticidal activity against Plutella xylostella, indicating that AST analogues may have a broader insecticidal spectrum. However, AstR-A’s potential as a promising target for broad-spectrum insect growth regulators (IGRs) has not yet been explored. In this study, phylogenetic analysis underscores its broad-spectrum insecticidal potential. Three-dimensional modeling of AstR-A in Ostrinia furnacalis, P. xylostella, Myzus persicae, and Acyrthosiphon pisum revealed conserved ligand-binding pockets. The peptide mimic A15 exhibited enhanced efficacy against O. furnacalis and A. pisum compared to fenoxycarb and pymetrozine while maintaining activity against P. xylostella and M. persicae. Its mechanism involved disrupting JH/20-hydroxyecdysone biosynthesis and cuticle formation, leading to pest death. These findings highlighted AstR-A as a promising target and A15 as an eco-friendly broad-spectrum IGR candidate.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"62 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quinazoline Alkaloids for Plant Protection: Design, Synthesis, and Biological Evaluation of Novel Deoxyvasicinone Derivatives 植物保护用喹唑啉类生物碱：新型脱氧水杨桃酮衍生物的设计、合成和生物学评价

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.4c11009

Tingyi Li, Yubin Xu, Sijia Liu, Yuliang Wu, Aidang Lu, Hongying Tang, Zhenghong Zhou, Qingmin Wang, Ziwen Wang

{"title":"Quinazoline Alkaloids for Plant Protection: Design, Synthesis, and Biological Evaluation of Novel Deoxyvasicinone Derivatives","authors":"Tingyi Li, Yubin Xu, Sijia Liu, Yuliang Wu, Aidang Lu, Hongying Tang, Zhenghong Zhou, Qingmin Wang, Ziwen Wang","doi":"10.1021/acs.jafc.4c11009","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c11009","url":null,"abstract":"For many years, plant diseases brought about by viruses plus fungi have led to significant declines in crop yield as well as quality, highlighting the urgent need for the development of effective antiviral as well as fungicidal agents. Quinazolinone alkaloids have an important role in drug research and development (R&D). Herein, alkaloid deoxyvasicinone was chosen as the mother structure; deoxyvasicinone derivatives were designed, prepared, and evaluated for their antiviral and antifungal activities. The antiviral activity against tobacco mosaic virus (TMV) suggested most compounds displayed better inhibitory effects compared to ribavirin; especially, compounds 3a, 3d, 4, and 5 with significantly higher antiviral activities (AAs) (EC50 values: 113–208 μg/mL) than ningnanmycin (EC50 value: 229 μg/mL) emerged as novel antiviral leads. Compound 4 was chosen for the antiviral action mechanism study, which suggested that it can interfere with the formation of 20S coat protein (CP) discs, thereby affecting the TMV particles’ assembly. In vitro antifungal activity research suggested most compounds exhibited wide-spectrum fungicidal activities (FAs) at a test concentration of 50 μg/mL. Further pesticide-likeness research exhibited that compounds 3a, 3d, 4, and 5 showed pesticide-likeness with the potential to be developed as pesticide candidates. This research establishes a basis for the use of deoxyvasicinone alkaloids in green pesticides.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"43 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives on Oleogels for Multifaceted Applications 多方位应用的油凝胶展望

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c02662

Natarajan Anbuselvan, Saritha Balasubramani, Yadavali Siva Prasad, Krishnamoorthy Lalitha

引用次数: 0

Lactiplantibacillus plantarum WH021 Alleviates Lipopolysaccharide-Induced Neuroinflammation via Regulating the Intestinal Microenvironment and Protecting the Barrier Integrity 植物乳杆菌WH021通过调节肠道微环境和保护屏障完整性减轻脂多糖诱导的神经炎症

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c00825

Bao-Lin He, Rui Cui, Teng-Gen Hu, Hong Wu

{"title":"Lactiplantibacillus plantarum WH021 Alleviates Lipopolysaccharide-Induced Neuroinflammation via Regulating the Intestinal Microenvironment and Protecting the Barrier Integrity","authors":"Bao-Lin He, Rui Cui, Teng-Gen Hu, Hong Wu","doi":"10.1021/acs.jafc.5c00825","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c00825","url":null,"abstract":"Neuroinflammation is one of the major pathological factors leading to neurodegenerative diseases (NDs). Currently, drug treatment is the main strategy for controlling NDs, but long-term drug use has many side effects. Studies have shown that probiotics can regulate neuroinflammation via the microbiota-gut-brain axis, thereby preventing and alleviating NDs. Our early studies screened several strains with antineuroinflammatory properties through the established microglia-zebrafish fast-screening model, and preliminary animal experiments demonstrated that Lactiplantibacillus plantarum WH021 has good antineuroinflammatory activity. Still, its specific effects and mechanisms need to be further explored. Therefore, this study aimed to investigate the effects of three doses of L. plantarum WH021 on behavioral disorders, brain and intestinal inflammation, and their possible mechanism in model mice with lipopolysaccharide (LPS)-induced neuroinflammation. The results showed that L. plantarum WH021 significantly improved the depression-like state caused by neuroinflammation, inhibited brain inflammation and neuronal damage, and enhanced synaptic function and blood-brain barrier integrity after 28 days of intervention in the high-dose group (109 CFU/day). In addition, pathological damage and inflammation in the colon tissue were effectively alleviated, and the function of the intestinal barrier was improved. From the perspective of gut microbiota and serum metabolites, L. plantarum WH021 was also found to regulate intestinal homeostasis and increase serum metabolic levels of neuroactive components and neurotransmitter metabolites. Ultimately, 5-hydroxytryptaminergic synapses, GABAergic synapses, and the arachidonic acid pathway were identified as potential mechanisms exerting their ability to alleviate neuroinflammation. These findings may provide a possible strategy for preventing neuroinflammation-related diseases.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"1 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Yarrowia lipolytica for Enhanced Production of Naringenin-Derived Flavonoids: Apigenin and Acacetin 增脂耶氏菌代谢工程促进柚皮素类黄酮：芹菜素和阿卡乙素的生产

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c02857

Nahye Park, Yangsub Ham, Seungwoo Cha, Byeongseon Jang, Gyeongmin Kim, Seung-Ho Baek, Ji-Sook Hahn

{"title":"Metabolic Engineering of Yarrowia lipolytica for Enhanced Production of Naringenin-Derived Flavonoids: Apigenin and Acacetin","authors":"Nahye Park, Yangsub Ham, Seungwoo Cha, Byeongseon Jang, Gyeongmin Kim, Seung-Ho Baek, Ji-Sook Hahn","doi":"10.1021/acs.jafc.5c02857","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02857","url":null,"abstract":"Apigenin and acacetin are flavonoids with potent antioxidant, anti-inflammatory, and anticancer activities, making them attractive for pharmaceutical and nutraceutical applications. However, their low natural abundance presents a challenge to large-scale production. In this study, we engineered Yarrowia lipolytica for the de novo biosynthesis of apigenin and acacetin from naringenin. To enhance naringenin production from l-tyrosine, we introduced four heterologous genes, optimized the flux through the l-tyrosine pathway, and eliminated the competing homogentisate pathway. Malonyl-CoA availability was increased by downregulating FAS1 (fatty acid synthase 1), while erythrose-4-phosphate levels were elevated through overexpression of TKL1 (transketolase 1). Additionally, genes encoding flavone synthase and flavonoid 4′-O-methyltransferase were integrated to convert naringenin into apigenin, and subsequently apigenin into acacetin. Under fed-batch fermentation with an optimized carbon-to-nitrogen ratio, acacetin production reached 1.10 g/L─the highest titer reported to date in a microbial system. These results highlight Y. lipolytica as a promising chassis for scalable flavonoid biosynthesis.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"2 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactylome Profiling Reveals the Potential Role of Lysine Lactylation in Regulating Soybean Seed Quality 赖氨酸乳酸化在大豆种子品质调控中的潜在作用

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c02106

Chang Xu, Jun Li, Chang Dong, Yu Zhang, Hui Li, Tianshu Li, Xue Han, Zhen Song, Mingming Zhang, Candong Li, Mingliang Yang, Qingshan Chen, Zhaoming Qi

{"title":"Lactylome Profiling Reveals the Potential Role of Lysine Lactylation in Regulating Soybean Seed Quality","authors":"Chang Xu, Jun Li, Chang Dong, Yu Zhang, Hui Li, Tianshu Li, Xue Han, Zhen Song, Mingming Zhang, Candong Li, Mingliang Yang, Qingshan Chen, Zhaoming Qi","doi":"10.1021/acs.jafc.5c02106","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c02106","url":null,"abstract":"Lysine lactylation (Kla), a recently discovered post-translational modification (PTM) that influences gene expression, metabolic pathways, and cellular signaling. While the importance of Kla is well-documented in mammals, its presence and impact in plant systems, particularly in soybeans, remain largely unknown. This study initiates the first proteomic analysis of lactylation in both wild-type (ZYD) and cultivated-type (SN14) soybeans. We established the first global lysine lactylation proteome profile in soybean seeds, identifying 783 Kla sites across 327 proteins. Our analysis revealed that the wild variety showed higher levels of protein content, lactate content, and lactylation levels. Moreover, we observed distinct Kla patterns between wild and cultivated soybeans, primarily affecting the lipid, carbohydrate, amino acid, and energy metabolism. Intriguingly, our results showed that metabolites associated with oil biosynthesis were downregulated in wild soybeans, while proteins linked to storage protein pathways were significantly upregulated, suggesting that Kla may play a role in negatively regulating the oil content while enhancing the protein content in soybean seeds. Taken together, this research provides the first comprehensive evidence of lactylation in soybeans and suggests its role in regulating oil and protein content in seeds. The findings open new avenues for improving soybean seed quality through targeted metabolic engineering and breeding strategies.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"14 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Glucose Uptake Level by Black Corn-Derived Anthocyanins at the Small Intestinal α-Glucosidases and Different Types of Glucose Transporters 黑玉米花青素对小肠α-葡萄糖苷酶和不同类型葡萄糖转运体葡萄糖摄取水平的调节

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c04779

Taek-Woo Lee, Young-Bo Song, Choon Young Kim, Jong Hun Lee, Byung-Hoo Lee

{"title":"Regulation of Glucose Uptake Level by Black Corn-Derived Anthocyanins at the Small Intestinal α-Glucosidases and Different Types of Glucose Transporters","authors":"Taek-Woo Lee, Young-Bo Song, Choon Young Kim, Jong Hun Lee, Byung-Hoo Lee","doi":"10.1021/acs.jafc.5c04779","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c04779","url":null,"abstract":"Attenuating the postprandial glycemic spike is crucial for managing blood-glucose homeostasis and mitigating chronic diseases. The structural and functional properties of anthocyanins from black corn (Zea mays L.), and their role in modulating glucose uptake by regulating digestive enzymes and glucose transporters via their multiple hydroxyl groups, were investigated. Among the purified anthocyanins with significant antioxidant activity in ABTS+ and DPPH assays, cyanidin, pelargonidin, and peonidin were dominant, as indicated by HPLC-MS analysis. Based on the IC50 values, black corn anthocyanins effectively inhibited mammalian α-glucosidases, reducing glucose generation from α-1,4-linked glycemic carbohydrates. In a Caco-2 cell model, anthocyanins reduced the glucose-transport efficiency and downregulated the mRNA expression of key glucose-transporter-related genes. ICR male mice orally administered the purified anthocyanins showed significantly attenuated glycemic responses to α-1,4-linked carbohydrates (p < 0.05). Thus, black corn anthocyanins exhibit promising properties as functional ingredients for the regulation of postprandial blood glucose levels.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"36 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic and Weighted Gene Coexpression Network Analyses Reveal Superior Multitarget Mechanisms of Dihydroquercetin Derivatives over Dihydroquercetin in Alleviating Energy Metabolism and Neurological Disorders in Diabetic Zebrafish 转录组学和加权基因共表达网络分析揭示双氢槲皮素衍生物在减轻糖尿病斑马鱼能量代谢和神经系统疾病方面优于双氢槲皮素的多靶点机制

IF 6.1 1区农林科学

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-29 DOI: 10.1021/acs.jafc.5c03914

Yong Cheng, Beijun Ang, Dandan Ma, Zhiwei Li, Fengxian Guo, Zhaojun Wang, Qiuming Chen, Maomao Zeng, Jie Chen, Benu Adhikari, Liduan Yin, Tong Wang, Zongping Zheng, Zhiyong He

{"title":"Transcriptomic and Weighted Gene Coexpression Network Analyses Reveal Superior Multitarget Mechanisms of Dihydroquercetin Derivatives over Dihydroquercetin in Alleviating Energy Metabolism and Neurological Disorders in Diabetic Zebrafish","authors":"Yong Cheng, Beijun Ang, Dandan Ma, Zhiwei Li, Fengxian Guo, Zhaojun Wang, Qiuming Chen, Maomao Zeng, Jie Chen, Benu Adhikari, Liduan Yin, Tong Wang, Zongping Zheng, Zhiyong He","doi":"10.1021/acs.jafc.5c03914","DOIUrl":"https://doi.org/10.1021/acs.jafc.5c03914","url":null,"abstract":"Diabetes-induced impairments in energy metabolism and neurological function significantly compromise physiological functions, highlighting the importance of exploring natural product-based therapeutic strategies. This study systematically investigated the effects of dihydroquercetin (DHQ) and its two derivatives, 8-hydroxy-dihydroquercetin (H-DHQ) and dihydroquercetin-7-O-β-d-(4″-O-methyl)-glucoside (DHQ-MG), which were derived from fermentation by Beauveria bassiana, on energy metabolism and neurological disorders in diabetic zebrafish larvae. The results demonstrated that both H-DHQ and DHQ-MG significantly ameliorated the motor dysfunction and neurodevelopmental impairments more effectively than DHQ, with DHQ-MG exhibiting the most pronounced effects. Enzymatic activity assays and neurochemical analyses confirmed enhanced neuroprotection, while transcriptomic analysis and weighted gene coexpression network analysis (WGCNA) revealed that H-DHQ and DHQ-MG modulate distinct sets of genes and signaling pathways, contributing to their superior effects on lipid metabolism, immune function, and neuroprotection compared to DHQ. The identified biologically relevant genes may serve as potential targets for H-DHQ or DHQ-MG, helping to alleviate diabetes-induced metabolic imbalance and neurodegenerative disorders. The results offer valuable insights into the potential use of DHQ-derived natural products for treating and managing diabetes and its complications.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"129 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0