Acta biochimica et biophysica Sinica最新文献_第2页

PRMT1 alleviates isoprenaline-induced myocardial hypertrophy by methylating SRSF1.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-10 DOI: 10.3724/abbs.2024175

Zi Yan, Wenhui Zhao, Naixin Zhao, Yufeng Liu, Bowen Yang, Li Wang, Jingyi Liu, Deping Wang, Jin Wang, Xiangying Jiao, Jimin Cao, Jianguo Li

{"title":"PRMT1 alleviates isoprenaline-induced myocardial hypertrophy by methylating SRSF1.","authors":"Zi Yan, Wenhui Zhao, Naixin Zhao, Yufeng Liu, Bowen Yang, Li Wang, Jingyi Liu, Deping Wang, Jin Wang, Xiangying Jiao, Jimin Cao, Jianguo Li","doi":"10.3724/abbs.2024175","DOIUrl":"https://doi.org/10.3724/abbs.2024175","url":null,"abstract":"Myocardial hypertrophy (MH) is an important factor contributing to severe cardiovascular disease. Previous studies have demonstrated that specific deletion of the protein arginine methyltransferase 1 (PRMT1) leads to MH, but the exact mechanism remains unclear. Serine/arginine-rich splicing factor 1 (SRSF1) affects the development and progression of cardiovascular disease by selectively splicing downstream signaling proteins. The present study is designed to determine whether PRMT1 is involved in MH by regulating SRSF1 and, if so, to explore the underlying mechanisms. Adult male mice and H9C2 cardiomyocytes are treated with isoprenaline (ISO) to establish MH models. The expression levels of PRMT1 are significantly decreased in the ISO-induced MH models, and inhibiting PRMT1 worsens MH, whereas overexpression of PRMT1 ameliorates MH. SRSF1 serves as the downstream target of PRMT1, and its expression is markedly elevated in MH. Moreover, SRSF1 increases the mRNA expressions of CaMKIIδ A and CaMKIIδ B, decreases the mRNA expression of CaMKIIδ C by altering the selective splicing of CaMKIIδ, and further participates in MH. In addition, there is an interaction between PRMT1 and SRSF1, whereby PRMT1 reduces the phosphorylation level of SRSF1 via methylation, thus further altering its functional activity and eventually improving MH. Our present study demonstrates that PRMT1 relieves MH by methylating SRSF1, which is expected to provide a new theoretical basis for the pathogenic mechanism of MH and potential drug targets for reducing MH and associated cardiovascular disease.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of reference genes for quantitative real-time PCR analysis in exogenous hormone-treated Lycoris aurea.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-05 DOI: 10.3724/abbs.2024197

Wei Zhao, Ying Tian, Yi Wang, Yun Wu, Ziming Ren, Kehu Li

引用次数: 0

Coupling of alternative splicing and alternative polyadenylation.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-05 DOI: 10.3724/abbs.2024211

Xueying Zhang, Feiyan Liu, Yu Zhou

{"title":"Coupling of alternative splicing and alternative polyadenylation.","authors":"Xueying Zhang, Feiyan Liu, Yu Zhou","doi":"10.3724/abbs.2024211","DOIUrl":"https://doi.org/10.3724/abbs.2024211","url":null,"abstract":"RNA splicing and 3'-cleavage and polyadenylation (CPA) are essential processes for the maturation of RNA. There have been extensive independent studies of these regulated processing events, including alternative splicing (AS) and alternative polyadenylation (APA). However, growing evidence suggests potential crosstalk between splicing and 3'-end processing in regulating AS or APA. Here, we first provide a brief overview of the molecular machines involved in splicing and 3'-end processing events, and then review recent studies on the functions and mechanisms of the crosstalk between the two processes. On one hand, 3'-end processing can affect splicing, as 3'-end processing factors and CPA-generated polyA tail promote the splicing of the last intron. Beyond that, 3'-end processing factors can also influence the splicing of internal and terminal exons. Those 3'-end processing factors can also interact with different RNA-binding proteins (RBPs) to exert their effects on AS. The length of 3' untranslated region (3' UTR) can affect the splicing of upstream exons. On the other hand, splicing and CPA may compete within introns in generating different products. Furthermore, splicing within the 3' UTR is a significant factor contributing to 3' UTR diversity. Splicing also influences 3'-end processing through the actions of certain splicing factors. Interestingly, some classical RBPs play dual roles in both splicing and 3'-end processing. Finally, we discuss how long-read sequencing technologies aid in understanding the coordination of AS-APA events and envision that these findings may potentially promote the development of new strategies for disease diagnosis and treatment.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4 ⁺ TRM cells through upregulation of PLZF.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-05 DOI: 10.3724/abbs.2024220

Meng Zhang, Jiafeng Sha, Na Li, Jingjing Feng, Tianyun Shi, Yunxia Yu, Xiaoting Ren, Zhoufang Mei, Zhijun Jie

{"title":"Early RSV infection aggravates asthma-related Th2 responses by increasing the number of CD4 + TRM cells through upregulation of PLZF.","authors":"Meng Zhang, Jiafeng Sha, Na Li, Jingjing Feng, Tianyun Shi, Yunxia Yu, Xiaoting Ren, Zhoufang Mei, Zhijun Jie","doi":"10.3724/abbs.2024220","DOIUrl":"https://doi.org/10.3724/abbs.2024220","url":null,"abstract":"Respiratory syncytial virus (RSV) infection is correlated with the chronic pathogenesis and exacerbation of asthma. However, the mechanism remains unclear. In this study, acute and memory (Mem) asthma models with early RSV infection are established to explore the persistence of the effects of RSV infection on asthma. Intravascular injection of an anti-CD45 antibody is performed to define CD4 + TRM cells accurately. RSV infection has a sustained impact on asthma exacerbation for at least six weeks, with high Th2 cytokine secretion in lung tissue instead of IgE response-related B cells. CD45 -CD4 + TRM cells are positively correlated with RSV-related asthma exacerbation and severe airway inflammation. Mechanistically, overexpression of the transcription factor PLZF in vitro increases the number of CD4 + TRM cells, and conditional knockout of Zbtb16 (encoding PLZF) can decrease the number of CD4 + TRM cells to aggravate allergic inflammation and reduce Th2 responses. This study provides evidence for potential combined strategies that might benefit asthma patients.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skeletal muscle-derived musclin attenuates glycolysis, oxidative stress, and pulmonary hypertension through the NPR3/AKT/mTORC1 pathway.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-05 DOI: 10.3724/abbs.2024214

Xiongshan Sun, Jia Wang, Yi Xiao, De Li, Qiang Wang, Wei Guo, Yongjian Yang

{"title":"Skeletal muscle-derived musclin attenuates glycolysis, oxidative stress, and pulmonary hypertension through the NPR3/AKT/mTORC1 pathway.","authors":"Xiongshan Sun, Jia Wang, Yi Xiao, De Li, Qiang Wang, Wei Guo, Yongjian Yang","doi":"10.3724/abbs.2024214","DOIUrl":"https://doi.org/10.3724/abbs.2024214","url":null,"abstract":"Exercise ameliorates pulmonary hypertension (PH) progression. However, the underlying mechanisms are largely unclear. Musclin is an exercise-responsive myokine that exerts protective effects on cardiovascular diseases. The current study aims to explore the role of musclin in the development of PH. A monocrotaline (MCT)-induced mouse PH model is established. Adeno-associated virus serotype 6 (AAV6)-mediated gene transfer is used to induce musclin overexpression in skeletal muscle. Ultrasound and morphological analyses are utilized to assess the severity of PH. Cell viability assay, Ki-67 immunofluorescence staining, wound healing assay, and transwell assay are used to evaluate the proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs). We find that the musclin levels in both plasma and skeletal muscle are decreased in MCT-treated mice. The external expression of musclin in skeletal muscle ameliorates pulmonary arterial remodeling and right ventricular dysfunction. In vitro, musclin treatment suppresses hypoxia-induced glycolysis, oxidative stress, proliferation, and migration. Further experiments reveal that musclin inhibits mechanistic target of rapamycin complex 1 (mTORC1) activity in hypoxia-stimulated PASMCs and pulmonary arteries of MCT-treated mice. Reactivating mTORC1 abolishes the protective role of musclin against PH. Additionally, musclin enhances its interaction with natriuretic peptide receptor 3 (NPR3) in PASMCs. Silencing of NPR3 reverses the inhibitory effects of musclin on AKT phosphorylation, mTORC1 activity, glycolysis, oxidative stress, proliferation, and migration in hypoxia-challenged PASMCs. In conclusion, our study highlights the inhibitory role of musclin in the proliferation and migration of PASMCs and PH progression, thereby providing a novel potent therapeutic strategy for treating PH and partly clarifying the mechanism of exercise-mediated protection against PH.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardioprotective effect of Saussurea involucrata injection against Doxorubicin-induced cardiotoxicity by network pharmacology analysis and experimental verification.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-05 DOI: 10.3724/abbs.2024170

Ding Wang, Yu Jin, Mengyu Yang, Yajing Xue, Xiaotong Zhang, Yanli Guo, Xinzhi Li, Ketao Ma

{"title":"Cardioprotective effect of Saussurea involucrata injection against Doxorubicin-induced cardiotoxicity by network pharmacology analysis and experimental verification.","authors":"Ding Wang, Yu Jin, Mengyu Yang, Yajing Xue, Xiaotong Zhang, Yanli Guo, Xinzhi Li, Ketao Ma","doi":"10.3724/abbs.2024170","DOIUrl":"https://doi.org/10.3724/abbs.2024170","url":null,"abstract":"Doxorubicin (Dox) is widely utilized in the clinical treatment of various cancers. Despite its efficacy, Dox induces numerous adverse effects in humans with significant cardiotoxicity, posing a major limitation to its use. Saussurea involucrata injection (SII), derived from Saussurea involucrata, exhibits notable anti-inflammatory and anti-oxidative stress properties. However, its potential protective effects against Dox-induced cardiotoxicity (DIC) remain unexplored. In this study, we investigate the ability of SII to mitigate DIC and elucidate the underlying mechanisms through experimental research and network pharmacology analysis. Results from both in vitro and in vivo experiments reveal that SII treatment significantly improves Dox-induced cardiac dysfunction, reducing pathological alterations and fibrosis in cardiomyocytes. Moreover, SII has cardioprotective effects by diminishing the inflammation, oxidative stress, and apoptosis triggered by Dox. Network pharmacological analysis further shows that SII downregulates P53 protein expression by activating the AKT/MDM2 signaling pathway, thus attenuating DIC. In conclusion, this study confirms that SII mitigates DIC through downregulation of the AKT/MDM2/P53 signaling pathway, suggesting a promising therapeutic strategy for alleviating DIC.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple allostery in the regulation of PDGFR beta kinase activities.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-02 DOI: 10.3724/abbs.2024205

Yanfeng Zhang, Meimei Wang, Guangcan Shao, Qingbin Shang, Mengqiu Dong, Xiaohong Qin, Li-Zhi Mi

{"title":"Multiple allostery in the regulation of PDGFR beta kinase activities.","authors":"Yanfeng Zhang, Meimei Wang, Guangcan Shao, Qingbin Shang, Mengqiu Dong, Xiaohong Qin, Li-Zhi Mi","doi":"10.3724/abbs.2024205","DOIUrl":"https://doi.org/10.3724/abbs.2024205","url":null,"abstract":"Platelet-derived growth factor receptor beta (PDGFRβ), a type III receptor tyrosine kinase (RTK) with a featured kinase insert, regulates important cellular functions. Dysregulation of PDGFRβ is associated with cardiovascular and fibrosis diseases. Thus, its kinase activity needs to be precisely regulated under physiological conditions. Early studies demonstrated that its kinase is autoinhibited by its juxtamembrane segment and activated by transphosphorylation. However, additional mechanisms are required for the comprehensive regulation of the receptor kinase. Herein, we provide evidence that dimerization of activated kinases, autoinhibition by the kinase insert, and dimerization of inactive kinase, all contribute to the regulation of the receptor kinase. Moreover, we find such multiple allosteric regulation is also conserved in other type III RTKs, including colony stimulating factor 1 receptor (CSF1R). Impaired allosteric regulation of CSF1R is associated with malfunctions of microglia and demyelination of neurons in hereditary diffuse leukoencephalopathy with spheroids (HDLS).","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-resolution imaging atlas reveals the context-dependent role of pancreatic sympathetic innervation in diabetic mice.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-02 DOI: 10.3724/abbs.2024215

Qingqing Xu, Yuxin Chen, Xinyan Ni, Hanying Zhuang, Shenxi Cao, Liwei Zhao, Leying Wang, Jianhui Chen, Wen Z Yang, Wenwen Zeng, Xi Li, Hongbin Sun, Wei L Shen

{"title":"High-resolution imaging atlas reveals the context-dependent role of pancreatic sympathetic innervation in diabetic mice.","authors":"Qingqing Xu, Yuxin Chen, Xinyan Ni, Hanying Zhuang, Shenxi Cao, Liwei Zhao, Leying Wang, Jianhui Chen, Wen Z Yang, Wenwen Zeng, Xi Li, Hongbin Sun, Wei L Shen","doi":"10.3724/abbs.2024215","DOIUrl":"https://doi.org/10.3724/abbs.2024215","url":null,"abstract":"A better understanding of how sympathetic nerves impact pancreatic function is helpful for understanding diabetes. However, there is still uncertainty and controversy surrounding the roles of sympathetic nerves within the pancreas. To address this, we utilize high-resolution imaging and advanced three-dimensional (3D) reconstruction techniques to study the patterns of sympathetic innervation and morphology in the islets of adult wild-type (WT) and diabetic mice. Our data show that more than ~30% of α/β-cells are innervated by sympathetic nerves in both WT and diabetic mice. Additionally, sympathetic innervated α/β-cells are reduced in diet-induced obese (DIO) mice, whereas sympathetic innervated β-cells are increased in db/ db mice. In addition, in situ chemical pancreatic sympathetic denervation (cPSD) improves glucose tolerance in WT and db/ db mice but decreases glucose tolerance in DIO mice. In situ cPSD also enhances insulin sensitivity in diabetic mice without affecting WT mice. Overall, our findings advance our understanding of diabetes by highlighting the distinctive impact of pancreatic sympathetic innervation on glucose regulation.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A TRIM21-based method for targeted protein degradation.

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-11-28 DOI: 10.3724/abbs.2024179

Weikang Hu, Xiang Qiu, Yun Yang, Yaoyao Wu, Chengcheng Wang, Ronggui Hu, Chuanyin Li

引用次数: 0

A PCR-independent, annealing-free cloning method for the insertion of short DNA fragments. 一种不依赖 PCR 的无退火克隆方法，用于插入短 DNA 片段。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-11-26 DOI: 10.3724/abbs.2024088

Linbo Li, Jin Yan, Yuan Qi, Zhenglong Xiang, Na Jiang, Tongkang Yuan, Zhenyi Wang, Yuan Wang, Huaizhe Zhan, Shiyi Liu, Li Zhao, Jing Xu, Xiaowei Lei, Yuxuan Liu, Gui Wang, Jiayang Xie, Zhenming Guo, Chunhai Cai, Shan Bian

引用次数: 0