Acta biochimica et biophysica Sinica最新文献_第3页

Evolutionary analysis of paired box gene family and biological function exploration of Lr. Pax7 in lamprey ( Lethenteron reissneri). 配对盒基因家族的进化分析及Lr.Pax7的生物学功能探索灯鱼（Lethenteron reissneri）中的 Pax7。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-08-06 DOI: 10.3724/abbs.2024121

Ayqeqan Nurmamat, Zihao Yan, Yao Jiang, Haoran Guan, Ruyu Zhuang, Shuyuan Zhang, Yuesi Zhou, Min Xiu, Ya Pang, Ding Li, Liang Zhao, Xin Liu, Yinglun Han

引用次数: 0

lncRNA CYTOR promotes lung adenocarcinoma gemcitabine resistance and epithelial-mesenchymal transition by sponging miR-125a-5p and upregulating ANLN and RRM2. lncRNA CYTOR通过疏导miR-125a-5p并上调ANLN和RRM2，促进肺腺癌吉西他滨耐药和上皮-间质转化。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-08-05 DOI: 10.3724/abbs.2024113

Qijun Cao, Haixia Wang, Jialong Zhu, Chen Qi, Hairong Huang, Xiaoyuan Chu

引用次数: 0

FOXM1 mediates methotrexate resistance in osteosarcoma cells by promoting autophagy. FOXM1 通过促进自噬介导骨肉瘤细胞对甲氨蝶呤的耐药性。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-08-01 DOI: 10.3724/abbs.2024084

Luoyang Wang, Dongchang Zhai, Lei Tang, Hui Zhang, Xinlong Wang, Ning Ma, Xiaoyue Zhang, Mingguo Cheng, Ruowu Shen

引用次数: 0

Deciphering disease through glycan codes: leveraging lectin microarrays for clinical insights. 通过糖代码破译疾病：利用凝集素芯片洞察临床。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-08-01 DOI: 10.3724/abbs.2024123

Hangzhou Yang, Zihan Lin, Bo Wu, Jun Xu, Sheng-Ce Tao, Shumin Zhou

引用次数: 0

Structural basis for the inhibition of coronaviral main proteases by PF-00835231. PF-00835231 抑制冠状病毒主要蛋白酶的结构基础

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-07-29 DOI: 10.3724/abbs.2024122

Xuelan Zhou, Xiaolu Lu, Cheng Lin, Xiaofang Zou, Wenwen Li, Xiangyi Zeng, Jie Wang, Pei Zeng, Weiwei Wang, Jin Zhang, Haihai Jiang, Jian Li

{"title":"Structural basis for the inhibition of coronaviral main proteases by PF-00835231.","authors":"Xuelan Zhou, Xiaolu Lu, Cheng Lin, Xiaofang Zou, Wenwen Li, Xiangyi Zeng, Jie Wang, Pei Zeng, Weiwei Wang, Jin Zhang, Haihai Jiang, Jian Li","doi":"10.3724/abbs.2024122","DOIUrl":"https://doi.org/10.3724/abbs.2024122","url":null,"abstract":"The main protease (M pro) of coronaviruses plays a key role in viral replication, thus serving as a hot target for drug design. PF-00835231 is a promising inhibitor of SARS-CoV-2 M pro. Here, we report the inhibitory potency of PF-00835231 against SARS-CoV-2 M pro and seven M pro mutants (G15S, M49I, Y54C, K90R, P132H, S46F, and V186F) from SARS-CoV-2 variants. The results confirm that PF-00835231 has broad-spectrum inhibition against various coronaviral M pros. In addition, the crystal structures of SARS-CoV-2 M pro, SARS-CoV M pro, MERS-CoV M pro, and seven SARS-CoV-2 M pro mutants (G15S, M49I, Y54C, K90R, P132H, S46F, and V186F) in complex with PF-00835231 are solved. A detailed analysis of these structures reveals key determinants essential for inhibition and elucidates the binding modes of different coronaviral M pros. Given the importance of the main protease for the treatment of coronaviral infection, structural insights into M pro inhibition by PF-00835231 can accelerate the design of novel antivirals with broad-spectrum efficacy against different human coronaviruses.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141791631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-glycosylation of SARS-CoV-2 spike protein by host O-glycosyltransferase strengthens its trimeric structure. 宿主O-糖基转移酶对SARS-CoV-2尖峰蛋白的O-糖基化加强了其三聚体结构。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-07-26 DOI: 10.3724/abbs.2024127

Zhijue Xu, Han Zhang, Jiaqi Tian, Xin Ku, Rumeng Wei, Jingli Hou, Can Zhang, Fang Yang, Xia Zou, Yang Li, Hiroyuki Kaji, Sheng-Ce Tao, Atsushi Kuno, Wei Yan, Lin-Tai Da, Yan Zhang

{"title":"O-glycosylation of SARS-CoV-2 spike protein by host O-glycosyltransferase strengthens its trimeric structure.","authors":"Zhijue Xu, Han Zhang, Jiaqi Tian, Xin Ku, Rumeng Wei, Jingli Hou, Can Zhang, Fang Yang, Xia Zou, Yang Li, Hiroyuki Kaji, Sheng-Ce Tao, Atsushi Kuno, Wei Yan, Lin-Tai Da, Yan Zhang","doi":"10.3724/abbs.2024127","DOIUrl":"10.3724/abbs.2024127","url":null,"abstract":"Protein O-glycosylation, also known as mucin-type O-glycosylation, is one of the most abundant glycosylation in mammalian cells. It is initially catalyzed by a family of polypeptide GalNAc transferases (ppGalNAc-Ts). The trimeric spike protein (S) of SARS-CoV-2 is highly glycosylated and facilitates the virus's entry into host cells and membrane fusion of the virus. However, the functions and relationship between host ppGalNAc-Ts and O-glycosylation on the S protein remain unclear. Herein, we identify 15 O-glycosites and 10 distinct O-glycan structures on the S protein using an HCD-product-dependent triggered ETD mass spectrometric analysis. We observe that the isoenzyme T6 of ppGalNAc-Ts (ppGalNAc-T6) exhibits high O-glycosylation activity for the S protein, as demonstrated by an on-chip catalytic assay. Overexpression of ppGalNAc-T6 in HEK293 cells significantly enhances the O-glycosylation level of the S protein, not only by adding new O-glycosites but also by increasing O-glycan heterogeneity. Molecular dynamics simulations reveal that O-glycosylation on the protomer-interface regions, modified by ppGalNAc-T6, potentially stabilizes the trimeric S protein structure by establishing hydrogen bonds and non-polar interactions between adjacent protomers. Furthermore, mutation frequency analysis indicates that most O-glycosites of the S protein are conserved during the evolution of SARS-CoV-2 variants. Taken together, our finding demonstrate that host O-glycosyltransferases dynamically regulate the O-glycosylation of the S protein, which may influence the trimeric structural stability of the protein. This work provides structural insights into the functional role of specific host O-glycosyltransferases in regulating the O-glycosylation of viral envelope proteins.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hedy: a groundbreaking revelation of cartilage oxygen homeostasis. Hedy：软骨氧平衡的突破性发现。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-07-24 DOI: 10.3724/abbs.2024116

Haoliang Hu, Bapuchi La, Kerui Huang, Yanling Long, Linxi Chen

引用次数: 0

RNA structure in alternative splicing regulation: from mechanism to therapy. 替代剪接调控中的 RNA 结构：从机制到治疗。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-07-22 DOI: 10.3724/abbs.2024119

Nengcheng Bao, Zhechao Wang, Jiayan Fu, Haiyang Dong, Yongfeng Jin

{"title":"RNA structure in alternative splicing regulation: from mechanism to therapy.","authors":"Nengcheng Bao, Zhechao Wang, Jiayan Fu, Haiyang Dong, Yongfeng Jin","doi":"10.3724/abbs.2024119","DOIUrl":"https://doi.org/10.3724/abbs.2024119","url":null,"abstract":"Alternative splicing is a highly intricate process that plays a crucial role in post-transcriptional regulation and significantly expands the functional proteome of a limited number of coding genes in eukaryotes. Its regulation is multifactorial, with RNA structure exerting a significant impact. Aberrant RNA conformations lead to dysregulation of splicing patterns, which directly affects the manifestation of disease symptoms. In this review, the molecular mechanisms of RNA secondary structure-mediated splicing regulation are summarized, with a focus on the complex interplay between aberrant RNA conformations and disease phenotypes resulted from splicing defects. This study also explores additional factors that reshape structural conformations, enriching our understanding of the mechanistic network underlying structure-mediated splicing regulation. In addition, an emphasis has been placed on the clinical role of targeting aberrant splicing corrections in human diseases. The principal mechanisms of action behind this phenomenon are described, followed by a discussion of prospective development strategies and pertinent challenges.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZIPK collaborates with STAT5A in p53-mediated ROS accumulation in hyperglycemia-induced vascular injury. ZIPK 与 STAT5A 合作，在高血糖诱导的血管损伤中参与 p53 介导的 ROS 积累。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-07-19 DOI: 10.3724/abbs.2024120

Qichao Wu, Tingting Xie, Chang Fu, Chenyu Sun, Yan Ma, Zhengzhe Huang, Jiao Yang, Xiaoxiao Li, Wenqian Li, Changhong Miao

{"title":"ZIPK collaborates with STAT5A in p53-mediated ROS accumulation in hyperglycemia-induced vascular injury.","authors":"Qichao Wu, Tingting Xie, Chang Fu, Chenyu Sun, Yan Ma, Zhengzhe Huang, Jiao Yang, Xiaoxiao Li, Wenqian Li, Changhong Miao","doi":"10.3724/abbs.2024120","DOIUrl":"https://doi.org/10.3724/abbs.2024120","url":null,"abstract":"In this study we investigate the role of Zipper-interacting protein kinase (ZIPK) in high glucose-induced vascular injury, focusing on its interaction with STAT5A and its effects on p53 and inducible nitric oxide synthase (NOS2) expression. Human umbilical vein endothelial cells (HUVECs) are cultured under normal (5 mM) and high (25 mM) glucose conditions. Protein and gene expression levels are assessed by western blot analysis and qPCR respectively, while ROS levels are measured via flow cytometry. ZIPK expression is manipulated using overexpression plasmids, siRNAs, and shRNAs. The effects of the ZIPK inhibitor TC-DAPK6 are evaluated in a diabetic rat model. Our results show that high glucose significantly upregulates ZIPK, STAT5A, p53, and NOS2 expressions in HUVECs, thus increasing oxidative stress. Silencing of STAT5A reduces p53 and NOS2 expressions and reactive oxygen species (ROS) accumulation. ZIPK is essential for high glucose-induced p53 expression and ROS accumulation, while silencing of ZIPK reverses these effects. Overexpression of ZIPK combined with STAT5A silencing attenuates glucose-induced alterations in p53 and NOS2 expression, thereby preventing cell damage. Coimmunoprecipitation reveals a direct interaction between ZIPK and STAT5A in the nucleus under high-glucose condition. In diabetic rats, TC-DAPK6 treatment significantly decreases ZIPK, p53, and NOS2 expressions. Our findings suggest that ZIPK plays a critical role in high glucose-induced vascular injury via STAT5A-mediated pathways, proposing that ZIPK is a potential therapeutic target for diabetic vascular complications.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and validation of disease severity-related circular RNA in acute pancreatitis. 鉴定和验证急性胰腺炎中与疾病严重程度相关的环状 RNA。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-07-19 DOI: 10.3724/abbs.2024115

Jiarong Li, Zefang Sun, Caihong Ning, Chiayen Lin, Dingcheng Shen, Gengwen Huang, hD P, Shuai Zhu, Lu Chen

引用次数: 0