Acta biochimica et biophysica Sinica最新文献

{"title":"Corrigendum to: PTBP1 promotes IRES-mediated translation of cyclin B1 in cancer.","authors":"Xinyi Fan, Zitong Zhao, Liying Ma, Xuanlin Huang, Qimin Zhan, Yongmei Song","doi":"10.3724/abbs.2025248","DOIUrl":"10.3724/abbs.2025248","url":null,"abstract":"","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"2150"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145814598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNAJC9 promotes cervical cancer cell proliferation by regulating GLI1 expression.","authors":"Zhongyi Xie, Longjiang Di, Xingkai He, Mingzhu Huang, Jinqin Qian, Hui Yang, Jiayi Chen, Xiaopeng Lu, Wei-Guo Zhu","doi":"10.3724/abbs.2026080","DOIUrl":"10.3724/abbs.2026080","url":null,"abstract":"<p><p>DNAJC9, an HSP40 family member with histone chaperone function, exhibits unclear roles in cervical cancer. DNAJC9 is specifically overexpressed in malignant cervical cancer cells, and downregulation of DNAJC9 inhibits proliferation, induces G1/S arrest, and suppresses tumorigenicity. GLI1 has been identified as a key downstream effector of DNAJC9, and GLI1 rescue reverses proliferation defects. Mechanistically, DNAJC9 promotes the p300-H3 interaction to sustain H3K27ac at the GLI1 enhancer and facilitate GLI1 transcription, driving proliferation. Furthermore, DNAJC9 expression correlates positively with GLI1 in clinical specimens, suggesting that the DNAJC9-GLI1 axis is a potential prognostic marker and therapeutic target.</p>","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":"58 ","pages":"1961-1975"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148012633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a colloidal gold immunochromatographic strip based on GAPDH for <i>Pentatrichomonas</i> <i>hominis</i> in dogs.","authors":"Qian Zhai, Xuejiao Chen, Xichen Zhang, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Nan Zhang","doi":"10.3724/abbs.2026043","DOIUrl":"10.3724/abbs.2026043","url":null,"abstract":"","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":"xx xx","pages":"2133-2138"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148012787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to: Decreased dynamin-related protein 1-related mitophagy induces myocardial apoptosis in the aging heart.","authors":"Xin Wei, Ye Wu, Wen Wang, Suli Zhang, Dan Liu, Huirong Liu","doi":"10.3724/abbs.2026146","DOIUrl":"10.3724/abbs.2026146","url":null,"abstract":"","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"2149"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A flavonoid-rich medicinal herb extract ameliorates high-fat diet-induced obesity and insulin resistance in mice.","authors":"Mengyu Li, Haiying Zong, Xiaoqin Yang, Qingqing Liu, Junjie Wang, Menglei Wang, Dengfang Wu, Shujie Zheng, Han Wang, Qiaoming Long","doi":"10.3724/abbs.2026007","DOIUrl":"10.3724/abbs.2026007","url":null,"abstract":"<p><p>Obesity has emerged as a critical global health challenge, contributing to severe metabolic and neoplastic complications. However, most existing anti-obesity drugs exhibit significant adverse effects, necessitating the development of safer therapeutic alternatives. In this study, we evaluate the efficacy and safety of a flavonoid-rich medicinal herb extract (MHE) in a high-fat diet (HFD)-induced murine obesity model. Daily oral administration of MHE does not alter food intake or induce hepatic injury but significantly attenuates HFD-induced weight gain ( <i>P</i> < 0.05) and adiposity accumulation. Furthermore, MHE treatment improves systemic insulin sensitivity and glycemic control. Notably, MHE enhances whole-body energy expenditure, as evidenced by elevated oxygen consumption (VO ₂), carbon dioxide production (VCO ₂), and heat generation ( <i>P</i> < 0.01). Mechanistically, MHE selectively promotes the proliferation of beneficial gut microbiota, including <i>Lactobacillus</i>, <i>Akkermansia</i>, and <i>Bifidobacterium</i> species, resulting in increased production of the short-chain fatty acid propionate (PA). Elevated circulating PA levels subsequently stimulate the browning/beiging of inguinal white adipose tissue (iWAT) and upregulate thermogenic pathways. Collectively, our findings demonstrate that MHE exerts anti-obesity effects through gut microbiota modulation and adipose tissue remodeling, offering a promising natural alternative for obesity management.</p>","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"2007-2019"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147343211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR2916-p5 and miR6478 from <i>Forsythia suspensa</i> fruit suppress inflammatory responses by targeting AKT signaling in human colorectal epithelial cells.","authors":"Dan Bai, Md Nur Islam, Dongwan Cheng, Han Feng, Tao Xu","doi":"10.3724/abbs.2026004","DOIUrl":"10.3724/abbs.2026004","url":null,"abstract":"<p><p>Cross-kingdom regulation by microRNAs (miRNAs) has been widely observed across species. Plant-derived miRNAs can be absorbed through the mammalian gastrointestinal tract, subsequently enter the bloodstream, and reach various target tissues. However, the specific composition, types, and functions of miRNAs in traditional Chinese medicinal herbs, such as <i>Forsythia suspensa</i>, remain unexplored. In this study, we identify 225 conserved miRNAs and 121 novel miRNAs in <i>F</i>. <i>suspensa</i> fruit by high-throughput sequencing. Target gene prediction of abundant <i>Forsythia</i>-derived miRNAs reveals that multiple downstream targets of fsu-miR2916-p5 and fsu-miR6478 are components of the human PI3K/AKT pathway, suggesting this pathway may serve as a key regulatory axis co-modulated by <i>Forsythia</i>-derived miRNAs. Overexpression of fsu-miR2916-p5 and fsu-miR6478 in human colorectal epithelial cells DLD1 suppresses the expression of AKT1 and AKT3, as well as the levels of phosphorylated AKT (p-AKT), as demonstrated by western blot analysis. Furthermore, RNA sequencing reveals downregulation of pro-inflammatory genes. RT-qPCR confirms the presence of <i>Forsythia</i>-derived miRNAs in the mouse colon after oral administration of <i>F</i>. <i>suspensa</i> extract, and confocal microscopy analysis validates their accumulation in colonic cells following gavage of synthetic <i>Forsythia</i>-derived miRNAs. Moreover, uptake of synthetic fsu-miR2916-p5 and fsu-miR6478 in DLD1 cells attenuates LPS-induced mitochondrial reactive oxygen species (ROS) production and reduces the expression of inflammation-associated molecules, including ICAM1, TGFBR1, and IL1R1, as assessed by immunofluorescence and western blot analysis. These findings identify miRNAs in <i>F</i>. <i>suspensa</i> and elucidate their regulatory influence on human gene expression and anti-inflammatory activity, thus offering a novel perspective on miRNA-mediated, plant-based therapeutic strategies for colitis.</p>","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"1976-1987"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147519586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E3 ubiquitin ligase COP1-mediated GSK3β degradation activates β-catenin signaling to facilitate colorectal cancer cell proliferation.","authors":"Dakui Luo, Kangjunjie Wang, Yufei Yang, Qingguo Li, Xinxiang Li","doi":"10.3724/abbs.2026023","DOIUrl":"10.3724/abbs.2026023","url":null,"abstract":"","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"2144-2148"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic subpopulations and spatial interactions of hepatic stellate cells and cancer-associated fibroblasts in liver cancer.","authors":"Wenjing Jiang, Wenjing Wei, Shuo Tu, Jinhua Yan, Xiaohua Yan","doi":"10.3724/abbs.2026138","DOIUrl":"10.3724/abbs.2026138","url":null,"abstract":"<p><p>Liver cancer, especially hepatocellular carcinoma (HCC), has become a global health-care challenge due to its high incidence and extremely poor prognosis. The tumor microenvironment (TME) plays a crucial role in cancer initiation, progression and therapy resistance. Although combination immunotherapies have revolutionized the clinical management of advanced HCC, therapeutic resistance still develops in many patients, which is partially attributed to the immunosuppressive effects of hepatic stellate cells (HSCs) and/or cancer-associated fibroblasts (CAFs) and the physical matrix barrier they create. As major components of the TME, HSCs are a major source of CAFs in liver cancer, and they both engage in fibrogenesis and cancer progression. However, non-specific CAF-targeting strategies demonstrate limited efficacy owing to the heterogeneity of CAFs in terms of transcriptional programs, phenotypes and functions. Advances in single-cell and spatial multi-omics techniques not only reveal the heterogeneity and functions of various subgroups of HSCs and CAFs, but also delineate the integrative spatiotemporal regulatory landscape of HSCs and CAFs in the TME. In this review, we summarize the specific biomarkers and diverse functions of distinct HSC and CAF subpopulations in liver cancer, highlighting the transitions between different states and their driving signals. We also focus on the spatial distribution and cellular interactions of HSCs and CAFs with their neighboring cells in liver cancer revealed by spatial transcriptomics and proteomics. Collectively, these insights into HSCs and CAFs provide potentially feasible strategies to alleviate resistance to immune checkpoint inhibitors and offer potential novel targets for liver cancer treatment.</p>","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"1931-1948"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the Zbp1-Ripk3 axis attenuates ischemic neuronal injury by suppressing PANoptosis.","authors":"Jun Shu, Hanwen Zhang, Aijuan Yan, Nan Li, Wenshi Wei, Li Zhang, Lirong Zhao","doi":"10.3724/abbs.2025177","DOIUrl":"10.3724/abbs.2025177","url":null,"abstract":"<p><p>Cerebral ischemia-reperfusion injury (CIRI) significantly exacerbates neuronal damage following ischemic stroke. Emerging evidence implicates PANoptosis, a novel inflammatory form of programmed cell death, in CIRI pathogenesis. However, the role of Z-DNA-binding protein 1 (Zbp1), a key regulator of PANoptosis and innate immune responses, remains poorly understood in this context. Here, we establish a middle cerebral artery occlusion/reperfusion (MCAO/R) mouse model and an oxygen-glucose deprivation/re-oxygenation (OGD/R) model in which HT-22 cells are used to simulate CIRI and investigate the effects of Zbp1. Western blot analysis is performed to assess Zbp1 and PANoptosis-related protein expressions. Cell viability and death are evaluated using Hoechst 33342 staining and Calcein AM/PI assays, and the cerebral infarct volume is quantified using 2,3,5-triphenyltetrazolium chloride staining. Immunoprecipitation and mass spectrometry identify Ripk3 as a potential downstream effector of Zbp1. Furthermore, Zbp1 and PANoptosis-related proteins are significantly upregulated following OGD/R treatment. <i>Zbp1</i> knockdown markedly reduces PANoptosis and cell injury in both models, decreases infarct volume and improves neurological outcomes in MCAO/R model mice. Conversely, Zbp1 overexpression exacerbates OGD/R-induced neuronal injury and PANoptosis in HT-22 cells. This effect is partially reversed by <i>Ripk3</i> knockdown, indicating that Ripk3 mediates Zbp1-induced neuronal damage. These findings highlight Zbp1 as a promising therapeutic target in ischemic stroke and underscore the need for further research into Zbp1-mediated PANoptotic pathways to aid in the development of novel neuroprotective strategies.</p>","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"2020-2032"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145832043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H-NS enhances phage resistance in <i>Klebsiella pneumoniae</i> by suppressing quorum sensing.","authors":"Yajing Song, Changhui Huang, Hailong Ou, Kai Zhou","doi":"10.3724/abbs.2025218","DOIUrl":"10.3724/abbs.2025218","url":null,"abstract":"<p><p>The molecular mechanisms underlying <i>Klebsiella pneumoniae</i> (KP) resistance to phages have not been fully elucidated, especially those involving the quorum-sensing (QS) system and the global regulator H-NS. In this work, we investigate the relationship between H-NS and QS in phage resistance by detecting transcriptional variation under phage pressure with RT-qPCR. <i>LuxS</i> and <i>lsr</i> operon knockout strains are generated via CRISPR editing, and H-NS-overexpressing mutants are constructed using plasmid-based overexpression. We also determine phage susceptibility by measuring the efficiency of plating (EOP). The capsular uronic acid content and phage adsorption efficiency are evaluated. The results show that phage pressure strongly downregulates <i>luxS</i> and <i>lsr</i> operon gene expressions but upregulates the transcription of H-NS. Knockout of either <i>luxS</i>/ <i>lsr</i> operon genes or overexpression of H-NS results in diminished phage sensitivity and the downregulation of CPS synthase genes. However, these changes do not reduce the capsular uronic acid content or affect phage adsorption rates, suggesting that H-NS-mediated resistance is independent of capsular modulation. In addition, this H-NS-QS regulatory link is conserved between HvKP and CRKP. H-NS enhances phage resistance by suppressing QS through a mechanism independent of capsular polysaccharide modulation, as evidenced by unaltered phage adsorption and statistically insignificant changes in capsular uronic acid. This provides a novel explanation for a resistance mechanism involving intracellular defenses ( <i>e</i>. <i>g</i>., abortive infection) and provides possible avenues for improving phage therapy targeting multidrug-resistant <i>K</i>. <i>pneumoniae</i>.</p>","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"2125-2132"},"PeriodicalIF":4.5,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}