Acta biochimica et biophysica Sinica最新文献_第4页

Novel FGF21 analogues through structure-based optimization for therapeutic development. 基于结构优化的新型FGF21类似物用于治疗开发。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-25 DOI: 10.3724/abbs.2024227

Yiqing Guo, Yuxuan Bao, Zhichao Chen, Zhiheng Rao, Yongde Luo, Sheng Ye, Si Liu

引用次数: 0

Types of cell death in diabetic cardiomyopathy: insights from animal models. 糖尿病性心肌病的细胞死亡类型：来自动物模型的见解

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-25 DOI: 10.3724/abbs.2024213

Hongjiao Xu, Zhuang Yu, Jun Zhu, Haoran Liu, Xiangyuan Chen, Jihong Jiang, Minmin Zhu, Jinbao Li

{"title":"Types of cell death in diabetic cardiomyopathy: insights from animal models.","authors":"Hongjiao Xu, Zhuang Yu, Jun Zhu, Haoran Liu, Xiangyuan Chen, Jihong Jiang, Minmin Zhu, Jinbao Li","doi":"10.3724/abbs.2024213","DOIUrl":"https://doi.org/10.3724/abbs.2024213","url":null,"abstract":"Approximately one-tenth of the global population is affected by diabetes mellitus, and its incidence continues to rise each year. In China, 1.4 million patients die from diabetes-related complications every year. Additionally, approximately 26% of patients with diabetes develop diabetic cardiomyopathy, with heart failure being one of the main causes of death in these patients. However, early detection of diabetic cardiomyopathy has proven to be difficult in a clinical setting; furthermore, there are limited guidelines and targeted means of prevention and treatment for this disease. In recent years, several studies have provided evidence for the occurrence of various forms of regulated cell death in diabetic myocardial cells, including apoptosis, necroptosis, ferroptosis, and cuproptosis, which are closely linked to the pathological progression of diabetic cardiomyopathy. Although most research on diabetic cardiomyopathy is currently in the animal trial phase, the inhibition of these regulatory cell death processes can limit or slow down the progression of diabetic cardiomyopathy. Therefore, this review discusses the appropriate animal experimental models currently available for diabetic cardiomyopathy and evaluates the roles of apoptosis, necroptosis, ferroptosis, and cuproptosis in diabetic cardiomyopathy. We hope to provide new methods and ideas for future research in diabetic cardiomyopathy.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-based design of covalent nanobody binders for a thermostable green fluorescence protein. 耐热绿色荧光蛋白共价纳米体结合物的结构设计。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024233

Zhihao Yue, Yanfang Li, Hongmin Cai, Hebang Yao, Dianfan Li, Aimin Ni, Tingting Li

引用次数: 0

The host gene CSTF2 regulates HBV replication via HBV PRE-induced nuclear export. 宿主基因CSTF2通过HBV预诱导核输出调控HBV复制。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024216

Jinyu Wang, Jing Li, Wentao Xie, Zhongliang Shen, Jingwen Wu, Richeng Mao, Mengji Lu, Jiming Zhang

{"title":"The host gene CSTF2 regulates HBV replication via HBV PRE-induced nuclear export.","authors":"Jinyu Wang, Jing Li, Wentao Xie, Zhongliang Shen, Jingwen Wu, Richeng Mao, Mengji Lu, Jiming Zhang","doi":"10.3724/abbs.2024216","DOIUrl":"https://doi.org/10.3724/abbs.2024216","url":null,"abstract":"The persistent global burden of hepatitis B virus (HBV) infection has prompted ongoing investigations into host determinants of viral control. In this study, we investigate the regulatory influence of the host gene cleavage stimulation factor subunit 2 (CSTF2) on HBV replication dynamics. We demonstrate differential CSTF2 expression across the spectrum of HBV infection phases, with upregulated expression noted during the immune-reactive and inactive carrier states compared with the immune-tolerant phase. Notably, dose-responsive attenuation of HBV DNA, as well as surface and core protein levels, is observed subsequent to CSTF2 overexpression, whereas HBV RNA levels remain unaffected. Upon HBV transfection, a notable alteration in CSTF2 subcellular localization is discerned, suggesting active relocalization to the cytoplasm, potentially mediated through interaction with the HBV posttranscriptional regulatory element (PRE). This interaction appears to impede the nuclear export of HBV RNA. Additionally, distinct antiviral efficacies are attributed to the functional domains of the CSTF2 protein, indicating a multifaceted host defense mechanism. These insights increase the understanding of host-virus interplay and identify CSTF2 as a candidate for antiviral therapeutic strategies.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

YTHDF2 influences hepatic fibrosis by regulating ferroptosis in hepatic stellate cells by mediating the expression of ACSL4 in an m ⁶A-dependent manner. YTHDF2通过介导ACSL4的表达，以m6a依赖的方式调节肝星状细胞的铁下垂，从而影响肝纤维化。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024162

Wentao Liu, Yuan He, Kunlun Chen, Jianwen Ye, Long Yu, Chuang Zhou, Wenlong Zhai

{"title":"YTHDF2 influences hepatic fibrosis by regulating ferroptosis in hepatic stellate cells by mediating the expression of ACSL4 in an m 6A-dependent manner.","authors":"Wentao Liu, Yuan He, Kunlun Chen, Jianwen Ye, Long Yu, Chuang Zhou, Wenlong Zhai","doi":"10.3724/abbs.2024162","DOIUrl":"https://doi.org/10.3724/abbs.2024162","url":null,"abstract":"Hepatic fibrosis (HF) is an abnormal reparative response of the liver to chronic injury and is histologically reversible. In recent years, increasing interest has been given to changes in m 6A in liver disease. In this study, we explore the role of the m 6A-modified reading protein YTHDF2 in HF and its regulatory mechanism. The HF mouse model is generated through CCl 4 injection, and the cell model is via TGF-β stimulation. The liver tissues are subjected to hematoxylin-eosin, Masson, and α-SMA immunohistochemical staining. Reactive oxygen species (ROS) and iron levels are examined via relevant kits. Quantitative real-time PCR, immunofluorescence staining, and western blot analysis were conducted to measure the YTHDF2 and ACSL4 levels. RNA immunoprecipitation, methylated RNA immunoprecipitation, RNA pull-down, and polysome fractionation were performed to understand the regulatory mechanism by which YTHDF2 affects ACSL4. The results show that YTHDF2 is highly expressed after HF induction, and the inhibition of YTHDF2 reduces fibrosis as well as ROS and iron levels. In vitro, overexpression of YTHDF2 increases hepatic stellate cell activation, as well as ROS and iron levels, and this effect is blocked by the silencing of ACSL4. YTHDF2 acts as a regulator of ACSL4 expression and is involved in m 6A modification. In addition, in vivo experiments indicate that overexpression of ACSL4 reverses the attenuating effect of YTHDF2 interference on HFs. Therefore, YTHDF2 mediates the expression of the ferroptosis marker protein ACSL4 in an m 6A-dependent manner, thereby affecting HF.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human umbilical cord mesenchymal stem cells enhance liver regeneration and decrease collagen content in fibrosis mice after partial hepatectomy by activating Wnt/β-catenin signaling. 人脐带间充质干细胞通过激活Wnt/β-catenin信号通路，促进肝部分切除后纤维化小鼠肝脏再生，降低胶原含量。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024207

Xuewei Li, Jinghui Feng, Haiqin Cheng, Ning Jin, Shanshan Jin, Zhizhen Liu, Jun Xu, Jun Xie

{"title":"Human umbilical cord mesenchymal stem cells enhance liver regeneration and decrease collagen content in fibrosis mice after partial hepatectomy by activating Wnt/β-catenin signaling.","authors":"Xuewei Li, Jinghui Feng, Haiqin Cheng, Ning Jin, Shanshan Jin, Zhizhen Liu, Jun Xu, Jun Xie","doi":"10.3724/abbs.2024207","DOIUrl":"https://doi.org/10.3724/abbs.2024207","url":null,"abstract":"Liver fibrosis is a critical stage in the progression of various chronic liver diseases to cirrhosis and liver cancer. Early inhibition of liver fibrosis is crucial for the treatment of liver disease. Hepatectomy, a common treatment for liver-related diseases, promotes liver regeneration. However, in the context of liver fibrosis, liver regeneration is hindered. Many studies have shown that mesenchymal stem cells (MSCs) can promote liver regeneration after partial hepatectomy (PH). However, there are few reports on the impact of MSC therapy on liver regeneration post-PH in the context of hepatic fibrosis. The objective of this study is to examine the impact of MSCs on liver regeneration following PH in the fibrotic liver and uncover the related molecular mechanisms. This study reveals that MSC therapy significantly enhances liver function and mitigates liver inflammation after PH in the context of hepatic fibrosis. MSCs also significantly promote liver regeneration and alleviate liver fibrosis. In addition, this study identifies the role of MSCs in promoting liver regeneration and alleviating liver fibrosis via the activation of Wnt/β-catenin signaling. The combination of MSCs with hepatectomy may offer a novel approach for the treatment of liver fibrotic diseases.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artemisinin alleviates arsenic-induced myocardial injury in rats by modulating oxidative stress and inflammatory responses. 青蒿素通过调节氧化应激和炎症反应减轻砷致大鼠心肌损伤。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024225

Wenjuan Qin, Yifei Zhou, Chuncui Chen, Xueting Guo, Ruimeng Tian, Ruoxi Chen, Wenrong Shi, Lei Huang, Caiyun Zhang, Shanshan Dong, Guilin Lu

引用次数: 0

Cellular functions and biomedical applications of circular RNAs. 环状rna的细胞功能和生物医学应用。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024241

Zheyu Zhang, Zefeng Wang

引用次数: 0

Inhibition of USP22 by miR-200b-5p represses gastric cancer cell proliferation and migration by targeting the NF-κB signaling pathway. miR-200b-5p抑制USP22通过靶向NF-κB信号通路抑制胃癌细胞的增殖和迁移。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-20 DOI: 10.3724/abbs.2024231

Yingying Guo, Panpan Zhang, Zhixing Gao, Xiaotian Liu, Chen Su, Su Chen, Tao An, Jingjing Hou

{"title":"Inhibition of USP22 by miR-200b-5p represses gastric cancer cell proliferation and migration by targeting the NF-κB signaling pathway.","authors":"Yingying Guo, Panpan Zhang, Zhixing Gao, Xiaotian Liu, Chen Su, Su Chen, Tao An, Jingjing Hou","doi":"10.3724/abbs.2024231","DOIUrl":"https://doi.org/10.3724/abbs.2024231","url":null,"abstract":"Gastric cancer (GC) is an aggressive tumor type with an intricate pathogenesis and limited therapeutic options. Ubiquitin-specific protease 22 (USP22) is a protein implicated in cell proliferation, metastasis, and tumorigenesis. However, the regulatory mechanisms governing USP22 in GC are still not fully understood. In this study, we perform bioinformatics analysis to identify conserved miRNA recognition sites for miR-200b-5p within the 3'UTR of USP22. Validation via luciferase reporter assay confirms the transcriptional regulation of USP22 by miR-200b-5p. Overexpression of miR-200b-5p markedly inhibits the proliferation and migration of GC cells in vitro and suppresses tumor growth in vivo. Conversely, ectopic expression of USP22 reversed this effect by modulating the NF-κB signaling pathway. Additionally, qPCR analysis reveals an inverse correlation between the miR-200b-5p level and USP22 expression in GC. Collectively, our findings indicate that miR-200b-5p-mediated inhibition of USP22 attenuates cell proliferation by targeting the NF-κB signaling pathway in GC, suggesting that miR-200b-5p and USP22 could serve as potential diagnostic or therapeutic targets for gastric cancer and other related human diseases.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-GlcNAcylation-related genes mediate tumor microenvironment characteristics and prediction of immunotherapy response in gastric cancer. o - glcn酰化相关基因介导胃癌肿瘤微环境特征及免疫治疗反应预测

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-18 DOI: 10.3724/abbs.2024222

Wangwen Wang, Xi Lu, Chengjun Zhu, Jie Li, Yue Liu, Zhangchao Yao, Xiaolin Li

{"title":"O-GlcNAcylation-related genes mediate tumor microenvironment characteristics and prediction of immunotherapy response in gastric cancer.","authors":"Wangwen Wang, Xi Lu, Chengjun Zhu, Jie Li, Yue Liu, Zhangchao Yao, Xiaolin Li","doi":"10.3724/abbs.2024222","DOIUrl":"https://doi.org/10.3724/abbs.2024222","url":null,"abstract":"We aim to identify molecular clusters related to O-GlcNAcylation and establish a novel scoring system for predicting prognosis and immunotherapy efficacy in patients with gastric cancer (GC). The transcriptomic and clinical data are obtained from XENA-UCSC and GEO databases. The O-GlcNAcylation-related genes are obtained from the GSEA database. Consensus clustering analysis is employed to identify O-GlcNAcylation-related molecular clusters, and principal component analysis (PCA) is utilized to develop a novel prognostic scoring system for predicting GC outcomes and immunotherapy efficacy. The prognostic accuracy of the scoring system is assessed across five real-world cohorts. The biological function of actin alpha 2, smooth muscle (ACTA2) in GC is determined through experimental verification. Using 34 O-GlcNAcylation-related genes associated with prognosis in GC patients, these individuals are divided into two distinct subgroups characterized by different outcomes, tumor microenvironment profiles, and clinical case characteristics. The DEGs between the two subgroups are subsequently used to further divide the GC patients into two subgroups by consensus cluster analysis. PCA is used to construct a prognostic scoring system, which reveal that patients in the low-score subgroup have a better prognosis and greater benefit from immunotherapy. The accuracy of the scoring system is confirmed through validation in a cohort of patients receiving immunotherapy in the real world. ACTA2 promotes proliferation and inhibits apoptosis in GC cells. These findings suggest that we successfully establish molecular clusters associated with O-GlcNAcylation and develop a scoring system that demonstrates strong performance in predicting the prognosis of patients with GC and the effect of immunotherapy interventions.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0