Animal Cells and Systems最新文献

CD47-mediated purification of human ventricular cardiomyocytes derived from pluripotent stem cells. 多能干细胞衍生的人心室心肌细胞的cd47介导纯化。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-04-20 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2657637

Soon-Jung Park, Hyung Kyu Choi, Keon Ha Park, Na Kyeong Park, Sung-Hwan Moon

{"title":"CD47-mediated purification of human ventricular cardiomyocytes derived from pluripotent stem cells.","authors":"Soon-Jung Park, Hyung Kyu Choi, Keon Ha Park, Na Kyeong Park, Sung-Hwan Moon","doi":"10.1080/19768354.2026.2657637","DOIUrl":"https://doi.org/10.1080/19768354.2026.2657637","url":null,"abstract":"Derivation of electrophysiologically mature cardiomyocytes from human pluripotent stem cells (hPSCs) remains a prerequisite for effective cardiac modeling and preclinical drug evaluation. However, current differentiation protocols often produce heterogeneous cell populations with limited maturity. In this study, we developed a CD47-based fluorescence-activated cell sorting strategy to purify ventricular-like cardiomyocytes from three-dimensionally differentiated hPSC-derived spheroids. CD47+ cardiomyocytes exhibited highly consistent contractile behavior and enhanced structural maturation, as confirmed using immunocytochemistry for myosin regulatory light chain 2 ventricular/cardiac muscle isoform and α-actinin. Electrophysiological analysis using a patch clamp revealed that the majority of CD47+ cells displayed ventricular-like action potential waveforms, characterized by prolonged repolarization duration, elevated amplitude, and a reduced negative maximum diastolic potential. Drug responsiveness was assessed using multi-electrode array recordings. CD47-enriched cardiomyocytes demonstrated reproducible and dose-dependent field potential alterations in response to known cardiotoxic agents, including remdesivir and quinidine, and outperformed metabolically purified controls in sensitivity and inter-replicate consistency analysis. These results establish CD47 as a reliable surface marker for isolating mature ventricular-like cardiomyocytes from hPSCs. The method enables the generation of functionally robust cardiomyocyte populations suitable for in vitro cardiac research and pharmacological testing.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"381-393"},"PeriodicalIF":3.2,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influences of pupal presence and worker number on the survival and development of pharaoh ant (Monomorium pharaonis) brood under laboratory conditions. 实验条件下蛹的存在和工蜂数对法老蚁（Monomorium pharaonis）幼虫生存和发育的影响。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-04-18 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2657636

Hee Jin Baek, Byunghyuk Kim

{"title":"Influences of pupal presence and worker number on the survival and development of pharaoh ant (Monomorium pharaonis) brood under laboratory conditions.","authors":"Hee Jin Baek, Byunghyuk Kim","doi":"10.1080/19768354.2026.2657636","DOIUrl":"https://doi.org/10.1080/19768354.2026.2657636","url":null,"abstract":"Social isolation and inadequate group composition can severely impair brood survival in eusocial insects due to the loss of social interactions. Establishing small artificial colonies is a prerequisite for studying developmental biology and functional genetics in ants; however, the minimal social requirements for effective brood rearing remain poorly understood. In this study, we investigated the impact of social composition - specifically the presence of pupae and worker abundance - on the survival and development of intact eggs within laboratory-maintained sub-colonies of the pharaoh ant, Monomorium pharaonis. Our analysis revealed that supplementing colonies with pupae significantly enhanced brood survival and accelerated larval development. Furthermore, we identified that sufficient worker numbers improve brood success, supporting the existence of a viable colony size. Our data suggest that while a sufficient workforce is essential for brood care, further increases in worker number do not yield proportional gains in rearing efficiency under our laboratory conditions. These results indicate that establishing favorable rearing conditions depends on achieving a functional demographic balance, particularly through pupal supplementation and an appropriate worker ratio. This study highlights the vital role of colony demographics and pupal presence in ant development, providing a practical framework for brood manipulation and ensuring baseline survival for future functional genetic applications.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"394-405"},"PeriodicalIF":3.2,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manic switching from depression is affected by adult hippocampal neurogenesis. 抑郁症的躁狂转换受成人海马神经发生的影响。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-04-08 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2654260

Jieun Song, Woonryong Kim, Yoojin Park, Ja-Myong Lee, Jong-Hoon Kim, Dong-Hyuk Park, Heon-Jeong Lee, Ki-Soon Shin, Hyun Jung Kim, Dongho Geum

{"title":"Manic switching from depression is affected by adult hippocampal neurogenesis.","authors":"Jieun Song, Woonryong Kim, Yoojin Park, Ja-Myong Lee, Jong-Hoon Kim, Dong-Hyuk Park, Heon-Jeong Lee, Ki-Soon Shin, Hyun Jung Kim, Dongho Geum","doi":"10.1080/19768354.2026.2654260","DOIUrl":"https://doi.org/10.1080/19768354.2026.2654260","url":null,"abstract":"Many individuals who initially present with depression later develop manic or hypomanic episodes, yet the biological processes accompanying this delayed shift remain unclear. Here, we examined whether perturbation of the adult hippocampal neurogenic niche is associated with time-dependent shifts in mood-relevant behaviors. Using Nestin-CreER::Rosa26-FloxedSTOP-DTa (Nestin-DTa) mice, we induced partial ablation of Nestin-positive adult neural stem cells (aNSCs) in the hippocampus by tamoxifen administration and assessed phenotypes at 4 and 8 weeks post-ablation. Neurogenic-lineage populations in the dentate gyrus were quantified by immunohistochemistry using stage-specific markers. Partial aNSC ablation reduced the Nestin-positive pool and BrdU-labeled proliferation, followed by a compensatory expansion of proliferative progenitors and DCX-positive neuroblasts. At 4 weeks, Nestin-DTa mice exhibited anxiety-like behavior and increased forced-swim immobility. By 8 weeks, anxiety-like measures normalized, and mice displayed increased exploratory/locomotor activity and reduced forced-swim immobility, accompanied by sustained elevation of progenitor and immature neuronal compartments. These findings provide a tractable model in which disruption of the adult neurogenic niche is associated with a transition from depression-like behavior to a hyperactive, mania-like phenotype over time.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"368-380"},"PeriodicalIF":3.2,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative stress and metabolic dysfunction in oral lichen planus pathogenesis. 氧化应激和代谢功能障碍在口腔扁平苔藓发病机制中的作用。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-04-01 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2648907

Hyo-Jin Song, Kun-Hwa Kang, Jin-Seok Byun, Do-Yeon Kim

{"title":"Oxidative stress and metabolic dysfunction in oral lichen planus pathogenesis.","authors":"Hyo-Jin Song, Kun-Hwa Kang, Jin-Seok Byun, Do-Yeon Kim","doi":"10.1080/19768354.2026.2648907","DOIUrl":"https://doi.org/10.1080/19768354.2026.2648907","url":null,"abstract":"Oral lichen planus (OLP) is a chronic, immune-mediated inflammatory disease of the oral mucosa that can cause persistent pain and has a recognized, though relatively low, risk of malignant transformation. Recent studies have identified oxidative and nitrosative stress as key contributors to the pathophysiology of OLP. Excessive production of reactive oxygen and nitrogen species, combined with reduced antioxidant defenses, leads to cellular damage, including lipid peroxidation, protein modification, and DNA injury. This oxidative imbalance drives keratinocyte apoptosis and sustains inflammatory signaling, creating a pro-inflammatory tissue environment. In parallel, metabolic dysfunction is increasingly recognized in OLP, characterized by altered energy production and nutrient utilization that disrupt tissue homeostasis. These two processes are closely linked, as oxidative stress impairs metabolic pathways, while metabolic alterations further increase oxidative burden, forming a self-reinforcing cycle that perpetuates mucosal injury and chronic inflammation. Clinically, markers of oxidative stress and metabolic imbalance, particularly those detectable in saliva, show promise for non-invasive diagnosis and risk assessment. Therapeutic strategies aimed at restoring redox balance and improving metabolic regulation represent emerging avenues beyond conventional symptom management. Understanding the crosstalk between oxidative stress and metabolism offers new opportunities for precision medicine approaches to improve the diagnosis, treatment, and long-term outcomes of patients with OLP.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"353-367"},"PeriodicalIF":3.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification. 增强子rna介导的TDP-43在早期神经谱系规范中的转录调控。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-03-26 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2643998

Yeonju Jang, Hyohi Lee, Myunggeun Oh, Jiin Moon, Seung-Jin Kim, Jaeil Han, Jong-Lyul Park, Seung-Kyoon Kim

{"title":"Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification.","authors":"Yeonju Jang, Hyohi Lee, Myunggeun Oh, Jiin Moon, Seung-Jin Kim, Jaeil Han, Jong-Lyul Park, Seung-Kyoon Kim","doi":"10.1080/19768354.2026.2643998","DOIUrl":"10.1080/19768354.2026.2643998","url":null,"abstract":"TAR DNA-binding protein 43 (TDP-43) is a DNA- and RNA-binding protein that regulates gene expression by modulating transcription and RNA processing. It plays pivotal roles in neuronal development and function, and its mislocalization and aggregation are major pathological features of several neurodegenerative diseases. However, the regulatory mechanisms that control Tdp-43 expression and activity during the transition from embryonic stem cells (ESCs) to neural progenitor cells (NPCs) remain poorly understood. Through integrative epigenomic and transcriptomic analyses, we identified multiple intergenic and intragenic enhancers within and around the Tdp-43 locus that generate enhancer RNAs (eRNAs). These eRNAs exhibit dynamic, region-specific expression changes and modulate Tdp-43 transcription in a stage- and context-dependent manner. Specifically, a subset of eRNAs was highly expressed in ESCs and downregulated upon differentiation, while others were selectively retained or induced in NPCs, paralleling changes in enhancer usage and histone modification states. Targeted knockdown of these eRNAs decreased Tdp-43 expression and was accompanied by changes in the expression of pluripotency- and lineage-associated markers, without implying direct control over full differentiation trajectories. These findings uncover a previously unrecognized aspect of Tdp-43 transcriptional regulation and highlight the significance of enhancer dynamics in the epigenetic regulation of TDP-43 expression during early lineage specification.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"320-341"},"PeriodicalIF":3.2,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative phylotranscriptomics reveals glycolytic adaptation associated with prolonged larval development in the coastal slipper lobster Crenarctus bicuspidatus. 比较系统转录组学揭示了与沿海双爪螯虾长时间幼虫发育相关的糖解适应。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-03-21 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2643995

Hyeongwoo Choi, Hyeongju Choi, Chungkug Park, Won-Kyo Jung, Tae-Hoon Kim, Yun Keun An

{"title":"Comparative phylotranscriptomics reveals glycolytic adaptation associated with prolonged larval development in the coastal slipper lobster Crenarctus bicuspidatus.","authors":"Hyeongwoo Choi, Hyeongju Choi, Chungkug Park, Won-Kyo Jung, Tae-Hoon Kim, Yun Keun An","doi":"10.1080/19768354.2026.2643995","DOIUrl":"10.1080/19768354.2026.2643995","url":null,"abstract":"The molecular basis of ecological adaptation in marine crustaceans remains poorly understood, particularly in non-model species such as the coastal slipper lobster (Crenarctus bicuspidatus). This study presents the first comprehensive analysis of the mitochondrial genome and transcriptome of C. bicuspidatus using high-throughput sequencing. Comparative transcriptomic analysis across 13 crustacean species identified 250 single-copy orthogroups. Gene family evolution analysis revealed 28 gene family expansions and 152 contractions in C. bicuspidatus. KEGG enrichment analysis of the expanded gene families showed strong representation of energy-related pathways, particularly pyruvate metabolism. Furthermore, positive selection analysis identified several genes involved in pyruvate metabolism and the citrate cycle (TCA cycle), including specific amino acid substitutions in the pyruvate dehydrogenase complex, a key enzyme in energy conversion. These findings suggest that C. bicuspidatus has undergone coordinated genomic adaptations to enhance energy metabolism, likely to support the high energetic demands of its extended larval development. This study highlights glycolytic adaptation as a key driver of life-history diversification in slipper lobsters.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"346-356"},"PeriodicalIF":3.2,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetramethylthiuram disulfide induces stress granules and DNA damage through oxidative stress in human lung epithelial cells. 四甲基硫脲二硫醚通过氧化应激诱导人肺上皮细胞应激颗粒和DNA损伤。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-03-10 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2637273

Ma Lin, Sangsoo Lee, Jiyun Gwak, Jihyun Cha, Seongjin Hong, Eun-Mi Kim, Kee K Kim

{"title":"Tetramethylthiuram disulfide induces stress granules and DNA damage through oxidative stress in human lung epithelial cells.","authors":"Ma Lin, Sangsoo Lee, Jiyun Gwak, Jihyun Cha, Seongjin Hong, Eun-Mi Kim, Kee K Kim","doi":"10.1080/19768354.2026.2637273","DOIUrl":"https://doi.org/10.1080/19768354.2026.2637273","url":null,"abstract":"Tetramethylthiuram disulfide (TMTD), widely used in rubber manufacturing and agriculture, presents occupational inhalation hazards, yet its effects on human lung epithelial cells remain poorly characterized. Here, we investigated TMTD-induced cellular stress responses in A549 lung epithelial cells, focusing on stress granule formation, oxidative stress, and DNA damage. TMTD induced concentration-dependent cytotoxicity, with brief exposure producing effects comparable to continuous exposure, indicating persistent cellular damage. Using live-cell imaging with A549 G3BP1-GFP knock-in cells, we demonstrated that TMTD rapidly triggered SG formation within minutes, accompanied by marked eIF2α phosphorylation. TMTD exposure caused dramatic intracellular ROS accumulation and robust γ-H2AX phosphorylation. Antioxidant rescue experiments using N-acetylcysteine confirmed that oxidative stress directly drives SG formation and DNA damage. Repeated TMTD exposure significantly increased apoptotic cell populations, demonstrating that cells cannot recover from recurrent exposure. Our findings reveal a mechanistic cascade whereby TMTD induces oxidative stress, triggers SG formation as an adaptive response, causes DNA damage, and ultimately leads to apoptosis when cellular stress overwhelms protective mechanisms. This study establishes stress granule formation as a sensitive early biomarker for TMTD exposure and highlights significant respiratory health risks for workers in rubber and agricultural industries, supporting the need for re-evaluation of occupational exposure limits and implementation of stringent protective measures.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"295-306"},"PeriodicalIF":3.2,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated morphological and transcriptomic analysis of sucker development in Octopus minor. 小章鱼吸盘发育的形态学和转录组学综合分析。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-03-04 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2638580

Yeonji Kim, Chan-Jun Lee, Kyoung-Bin Ryu, Yam Prasad Aryal, Seonmi Jo, Dae-Cheol Seo, Ji-Hoon Song, Byeong-Gil Jeong, Mi-Jin Lee, Shin-Hong An, Seung-Hyun Jung, Hae-Youn Lee, Sung-Jin Cho

{"title":"Integrated morphological and transcriptomic analysis of sucker development in Octopus minor.","authors":"Yeonji Kim, Chan-Jun Lee, Kyoung-Bin Ryu, Yam Prasad Aryal, Seonmi Jo, Dae-Cheol Seo, Ji-Hoon Song, Byeong-Gil Jeong, Mi-Jin Lee, Shin-Hong An, Seung-Hyun Jung, Hae-Youn Lee, Sung-Jin Cho","doi":"10.1080/19768354.2026.2638580","DOIUrl":"10.1080/19768354.2026.2638580","url":null,"abstract":"Coleoid cephalopods are excellent models for evolutionary and developmental studies due to their centralized nervous system, short life span, and sophisticated sense organs. Arm suckers, essential for predation, manipulation, and locomotion, have been studied in Decapodiformes, but little is known at the cellular and molecular levels. Here, we investigated sucker development in Octopus minor from the embryo to the juvenile stage using morphological, histological, immunostaining, scanning electron microscopy (SEM), in situ hybridization, and transcriptomic analyses. SEM revealed that suckers initially form symmetrically and later become asymmetrical through an embedding process. Histology showed progressive structural differentiation, while immunostaining with acetylated α-tubulin and phalloidin visualized nerve fiber and muscle development. Transcriptome profiling of embryonic stages 12, 14, and 20 identified 2,349 differentially expressed genes (DEGs) linked to arm and muscle development. Among them, Omi-Gata4, Omi-Mef2A, and WNT signaling molecules (Omi-Fzd9, Omi-Wnt2, Omi-Wnt5) exhibited increased expression in arms and developing suckers, consistent with in situ hybridization results. These findings suggest that WNT signaling contributes to sucker muscle development in O. minor, paralleling its conserved role in vertebrates. This study provides new insights into the embryonic morphogenesis of arms and suckers, highlighting the molecular and cellular mechanisms that shape cephalopod appendages.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"307-319"},"PeriodicalIF":3.2,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147375598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Notch signaling in leech neurogenesis: expression patterns and functional insights in the Glossiphoniid leech Helobdella austinensis. Notch信号在水蛭神经发生中的作用：舌音水蛭Helobdella austinensis的表达模式和功能。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-02-23 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2631845

Geon-Hwi Jeong, In-Hyeok Pyo, Yam Prasad Aryal, Geon-Woo Lee, Kyoung-Bin Ryu, Hee-Jin Kwak, Sung-Jin Cho

{"title":"Notch signaling in leech neurogenesis: expression patterns and functional insights in the Glossiphoniid leech Helobdella austinensis.","authors":"Geon-Hwi Jeong, In-Hyeok Pyo, Yam Prasad Aryal, Geon-Woo Lee, Kyoung-Bin Ryu, Hee-Jin Kwak, Sung-Jin Cho","doi":"10.1080/19768354.2026.2631845","DOIUrl":"10.1080/19768354.2026.2631845","url":null,"abstract":"The Notch signaling pathway is one of the most evolutionarily conserved pathways, playing a crucial role in juxtacrine signaling between adjacent cells. Although previous studies have characterized Notch signaling in diverse models, its function in the lophotrochozoan animals is largely not understood and is only partially identified in early-stage embryos. To address this gap, we reaffirmed the expression of Notch homolog, including downstream components, in the organogenesis of the Glossiphoniid leech, Helobdella austinensis. We analyze the spatial and temporal expression patterns of the Notch receptor (Hau-notch1), ligands (Hau-delta and Hau-jagged), and transcription factors (Hau-hes and Hau-hey) from embryonic stages 8-11. Notch transcripts are expressed in the germinal plate and segmental precursor cells at stage 8, with expression expanding to the somites, ventral and supraesophageal ganglia by stage 9. In organogenesis stages (stages 9-11), Notch components are expressed in the anterior and posterior suckers, proboscis, and ventral ganglia, indicating their role in nerve differentiation. Furthermore, blocking of Notch signaling by DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycine t-butyl ester) leads to the disruption of nerve formation with downregulation of transcription factors (Hau-hes and Hau-hey). Our findings provide insights into the conserved mechanisms of Notch signaling in bilaterian neural development, contributing to a deeper understanding of evolutionary developmental biology.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"282-294"},"PeriodicalIF":3.2,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12983829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147462556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long non-coding RNAs in the exosomal network: dual roles and clinical implications in cancer. 外泌体网络中的长链非编码rna：癌症中的双重作用和临床意义。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-02-20 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2632447

Goeun Yoon, Seulhye Cho, Yu-Ri Choi, Jungwook Roh, Wanyeon Kim

{"title":"Long non-coding RNAs in the exosomal network: dual roles and clinical implications in cancer.","authors":"Goeun Yoon, Seulhye Cho, Yu-Ri Choi, Jungwook Roh, Wanyeon Kim","doi":"10.1080/19768354.2026.2632447","DOIUrl":"https://doi.org/10.1080/19768354.2026.2632447","url":null,"abstract":"Exosomes are a type of extracellular vesicles with a diameter of 30-150 nm, formed within multivesicular bodies of late endosomes, and released into the extracellular environment before being taken up by recipient cells. Exosomes transfer bioactive molecules, such as proteins and various RNAs, between cells and thereby influence recipient cell behavior. Among these molecules, long non-coding RNAs (lncRNAs) are not only selectively packaged into exosomes but have also been reported to participate in exosome biogenesis and secretion. Furthermore, they can contribute to cancer development by regulating gene expression through epigenetic mechanisms such as miRNA sponging, protein interactions, and methylation. These mechanisms appear to drive tumorigenesis by altering the tumor microenvironment, including macrophage polarization, immune evasion and suppression, angiogenesis, and lymphangiogenesis-mediated metastasis. In addition, exosomal lncRNAs can be readily detected in serum, highlighting their potential as biomarkers for liquid biopsy-based diagnosis. Finally, exosomal lncRNAs have been reported to contribute to drug resistance in various cancers. Therefore, therapeutic strategies targeting exosomal lncRNAs are expected to improve clinical outcomes in cancer patients.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"265-281"},"PeriodicalIF":3.2,"publicationDate":"2026-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12927404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0