Animal Cells and Systems最新文献

Comparative profiling of selected pattern recognition receptor (PRR) pathways reveals functional heterogeneity of monocytic models in the monocyte activation test. 选择模式识别受体（PRR）途径的比较分析揭示了单核细胞激活试验中单核细胞模型的功能异质性。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-29 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2749253

Ji-Youn Lim, Soyoung Jeon, Yeram Jeon, Mihye Kim, Hyungjung Koh, Seoyoung Wang, Young-Joo Jeon, Jangwook Lee, Sung-Jin Yoon, Wantae Kim, Boksik Cha

{"title":"Comparative profiling of selected pattern recognition receptor (PRR) pathways reveals functional heterogeneity of monocytic models in the monocyte activation test.","authors":"Ji-Youn Lim, Soyoung Jeon, Yeram Jeon, Mihye Kim, Hyungjung Koh, Seoyoung Wang, Young-Joo Jeon, Jangwook Lee, Sung-Jin Yoon, Wantae Kim, Boksik Cha","doi":"10.1080/19768354.2026.2749253","DOIUrl":"https://doi.org/10.1080/19768354.2026.2749253","url":null,"abstract":"The monocyte activation test (MAT) detects pyrogenic contamination from the cytokine response of human monocytes and is used as an alternative to the rabbit pyrogen test. PBMC are the reference cell source for the MAT; however, donor variability and limited supply have led to frequent substitutions with the cell lines THP-1, HL-60, and U937. Consequently, whether these lines reproduce PBMC behavior beyond that of endotoxins across receptor pathways remains unknown. In this study, we compared the IL-6 responses of PBMCs and cell lines to 10 pyrogenic stimuli acting through Toll-like receptors (TLRs) and NOD2. All four systems were assayed in parallel, and each stimulus was tested over a full dose range. Basal transcript levels of the corresponding receptors were measured using RT-qPCR in the same cells and compared with publicly available expression data. Detection limits, precision estimates, and endotoxin equivalent potencies were reported for each stimulus. PBMCs responded to every stimulus in the panel and produced the highest maximal IL-6 for most pathways; the cell lines responded only at higher ligand concentrations and over the compressed dose-response ranges. These results indicate that the relationship between primary and immortalized monocytic systems depends on the receptor engaged, rather than a fixed reduction in sensitivity. Performance against endotoxins does not predict performance against non-endotoxin pyrogens, which are the contaminants that MAT is used to detect.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"630-643"},"PeriodicalIF":3.6,"publicationDate":"2026-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13721825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149317221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USF2 deficiency enhances intracellular clearance of Salmonella Typhimurium via xenophagy and LC3-associated phagocytosis. USF2缺乏通过异种吞噬和lc3相关吞噬增强鼠伤寒沙门菌的细胞内清除率。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-21 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2721740

Junhee Kwon, Jina Jeon, Ji Won Kim, Hamin Bae, Jaebeom Kim, Young Suk Yu, Sung Hee Baek, Keun Il Kim

{"title":"USF2 deficiency enhances intracellular clearance of Salmonella Typhimurium via xenophagy and LC3-associated phagocytosis.","authors":"Junhee Kwon, Jina Jeon, Ji Won Kim, Hamin Bae, Jaebeom Kim, Young Suk Yu, Sung Hee Baek, Keun Il Kim","doi":"10.1080/19768354.2026.2721740","DOIUrl":"https://doi.org/10.1080/19768354.2026.2721740","url":null,"abstract":"The upstream stimulatory factor 2 (USF2) is a transcription factor known as a transcriptional activator that binds to E-box motifs in gene promoters. Recently, we reported that USF2 is also a transcriptional repressor of the genes that encode lysosomes and autophagy machinery, and that its deficiency results in the elevation of autophagy. The present study was designed to determine whether the absence of USF2 is responsible for more effective protection of the host cells from bacterial infection and intracellular bacterial growth. In this context, we employed Salmonella enterica serovar Typhimurium (S. Typhimurium) as a model pathogen and monitored the changes in intracellular bacterial burden observed in the wild-type (WT) cells and USF2-deficient cells over the time course of infection. The number of intracellular bacteria increased after infection in WT cells but remained similar or decreased in USF2-deficient cells. This phenomenon was observed in various cell types, including mouse embryonic fibroblasts (MEFs), RAW264.7 macrophage-like cells, and primary bone marrow-derived macrophages (BMDMs). The phagocytosis levels were the same in WT and USF2-deficient macrophages, but the latter had a higher basal autophagy, increased lysosome abundance, and increased expression of lysosomal genes. In addition, the components of LC3-associated phagocytosis (LAP), such as LC3B and RUBICON, together with reactive oxygen species (ROS) production, were elevated in USF2-deficient macrophages. Our results demonstrated that the enhanced autophagy in non-phagocytic cells and the elevated autophagy and LAP in phagocytes both contribute to the improved clearance of intracellular pathogens in USF2-deficient cells.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"616-629"},"PeriodicalIF":3.6,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13715337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149294154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural killer T cells as therapeutic targets for neuroinflammatory disorders. 自然杀伤T细胞作为神经炎性疾病的治疗靶点。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-18 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2746896

Hyun Jung Park, Sung Won Lee, Tae-Cheol Kim, Yun Hoo Park, Luc Van Kaer, Seokmann Hong

{"title":"Natural killer T cells as therapeutic targets for neuroinflammatory disorders.","authors":"Hyun Jung Park, Sung Won Lee, Tae-Cheol Kim, Yun Hoo Park, Luc Van Kaer, Seokmann Hong","doi":"10.1080/19768354.2026.2746896","DOIUrl":"https://doi.org/10.1080/19768354.2026.2746896","url":null,"abstract":"Natural killer T (NKT) cells are innate-like T lymphocytes that express natural killer (NK) cell receptors (NKRs), which recognize glycolipid antigens presented by the MHC class I-like protein CD1d, linking innate and adaptive immunity. NKT cells include two main subsets, type I and type II, which differ in TCR usage and antigen specificity. In addition, NKT-like cells, which express NKRs but are CD1d-independent, have attracted growing interest in neuroimmunology. These NKR-expressing T cells play important roles in either promoting or suppressing pathological conditions within the brain. This review overviews recent advances in understanding NKT cells in immune disorders of the central nervous system (CNS), including neurodegenerative diseases, neuroinflammation, autoimmune diseases, brain injury, and CNS malignancies, such as glioblastoma. By interacting with CD1d on non-immune cells (e.g. astrocytes) and immune cells (e.g. microglia) through their TCR, type I NKT cells help maintain or disrupt immune homeostasis in the CNS. In addition, exogenous ligands, such as alpha-galactosylceramide, can modify type I and type II NKT cell phenotypes and promote regulatory functions in NKT-like cells, which may mitigate excessive inflammation. This review provides insights into how NKT cell subsets could serve as therapeutic targets for neuroinflammatory diseases.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"600-615"},"PeriodicalIF":3.6,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13686197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149242675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matching developmental timelines of fish, frog, mouse and human brains. 匹配鱼、青蛙、老鼠和人类大脑的发育时间表。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-16 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2742604

Hyun Jung Park, Hosung Jung

引用次数: 0

Pharmacological inhibition of REV-ERBα ameliorates motor deficits in the 6-OHDA-lesioned mouse model of Parkinson's disease. rev - erba的药物抑制可改善6- ohda损伤的帕金森病小鼠模型的运动缺陷。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-15 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2690728

Jeongah Kim, Mijeong Choe, Sangwon Jang, Inah Park, Sungjoon Oh, Eun-Kyoung Kim, Youngshik Choe, Woong Sun, Kyungjin Kim

{"title":"Pharmacological inhibition of REV-ERBα ameliorates motor deficits in the 6-OHDA-lesioned mouse model of Parkinson's disease.","authors":"Jeongah Kim, Mijeong Choe, Sangwon Jang, Inah Park, Sungjoon Oh, Eun-Kyoung Kim, Youngshik Choe, Woong Sun, Kyungjin Kim","doi":"10.1080/19768354.2026.2690728","DOIUrl":"10.1080/19768354.2026.2690728","url":null,"abstract":"Parkinson's disease (PD) is characterized by nigrostriatal dopaminergic (DAergic) degeneration and motor dysfunction. Many PD patients also show circadian rhythm disturbances. Increasing evidence links circadian clock dysfunction to PD pathology. REV-ERBα is a circadian nuclear receptor that regulates DAergic genes under physiological conditions. However, its role in nigrostriatal dopamine (DA) homeostasis and motor function in PD remains unclear. In this study, we found elevated Rev-erbα mRNA expression in tyrosine hydroxylase (TH)-positive neurons of the substantia nigra (SN) in a 6-hydroxydopamine (6-OHDA) mouse model of PD. To test its functional role, we assessed the acute effects of local SN microinjection of the REV-ERBα antagonist SR8278. We found that SR8278 partially recovered motor performance and improved fine motor function. These effects occurred regardless of circadian timing. SR8278 administration did not restore TH expression or tissue DA levels in the striatum and SN. However, in vivo microdialysis showed enhanced extracellular DA turnover in the striatum. Specifically, while extracellular DA metabolites level increased, basal extracellular DA levels showed no change. Together, these findings identify REV-ERBα as a potential therapeutic target that modulates extracellular nigrostriatal DA dynamics and improves motor function in PD.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"558-571"},"PeriodicalIF":3.6,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic evaluation of AAV administration strategies for targeting Phox2b + primary visceral sensory neurons. 针对Phox2b +初级内脏感觉神经元的AAV给药策略的系统评价。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-15 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2749248

Su Young Ki, Ha Kyeong Kim, Hyun Ji Gu, Soo Min Oh, Dong-Hoon Kim, Yong Taek Jeong

{"title":"Systematic evaluation of AAV administration strategies for targeting Phox2b + primary visceral sensory neurons.","authors":"Su Young Ki, Ha Kyeong Kim, Hyun Ji Gu, Soo Min Oh, Dong-Hoon Kim, Yong Taek Jeong","doi":"10.1080/19768354.2026.2749248","DOIUrl":"10.1080/19768354.2026.2749248","url":null,"abstract":"Visceral sensory neurons (VSNs) play an essential role in interoception, but it remains challenging to selectively access them using genetic tools due to their anatomical location and shared molecular markers with central autonomic circuits. Although transgenic models visualizing Phox2b-expressing neurons have been developed to genetically label VSNs, concurrent Phox2b expression in the hindbrain limits their selectivity. To overcome these limitations, we engineered several Phox2b promoter-driven adeno-associated virus (AAV) vectors and evaluated the effects of capsid serotype, delivery route, and injection timing. Using systemic delivery of AAV-PHP.S-Phox2b-Cre, we found that postnatal day 8 is an optimal time window for efficient and selective labeling of VSNs with a relatively low dose. The result contrasts with neonatal administration, which led to extensive ectopic labeling at the same dose. Unexpectedly, lingual submucosal delivery of AAV-retro-Phox2b-Cre, initially intended to label tongue-innervating sensory neurons, also labeled most vagal neurons. These labeled neurons were AAV-retro capsid-specific, because local delivery of AAV-PHP.S-Phox2b-Cre labeled cells only near the injection site. Altogether, this study provides practical parameters for selective genetic access to VSNs using AAV.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"572-586"},"PeriodicalIF":3.6,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZEB1-mediated suppression of circDennd1b promotes the migration and invasion of lung cancer cells. zeb1介导的circDennd1b的抑制促进了肺癌细胞的迁移和侵袭。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-10-06 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2742602

Seongran Cho, Jin-A Choi, Jihye Park, Keunsoo Kang, Young-Ho Ahn

引用次数: 0

Chemical synchronization using household bleach can affect development, behavior and lifespan in Caenorhabditis elegans. 使用家用漂白剂的化学同步可以影响秀丽隐杆线虫的发育、行为和寿命。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-09-30 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2719247

Seung Hyun Kim, Joohong Ahnn, Heon Seok Kim, Sun-Kyung Lee

{"title":"Chemical synchronization using household bleach can affect development, behavior and lifespan in Caenorhabditis elegans.","authors":"Seung Hyun Kim, Joohong Ahnn, Heon Seok Kim, Sun-Kyung Lee","doi":"10.1080/19768354.2026.2719247","DOIUrl":"10.1080/19768354.2026.2719247","url":null,"abstract":"Caenorhabditis elegans, a nematode genetic model, is often synchronized in experiments to accurately observe its unbiased phenotypes. Chemical synchronization, simple bleaching of gravid worms, has been widely used to obtain eggs in bulk because the eggshell is extremely resistant to harsh conditions. While those eggs are considered to preserve their natural properties and are allowed to hatch to obtain synchronized worms, it has not been fully investigated whether chemical synchronization influences embryonic and post-embryonic traits. In this study, we compared some phenotypes observed in worms collected by picking laid eggs and by chemical synchronization. Chemical synchronization decreased hatching and developmental rates and, in some conditions, affected morphological traits, such as body length and head size, and brood size. In contrast, chemical synchronization did not affect the developmental potential of early- or later-stage eggs, or chemosensory behaviors. While the lifespan of wild-type worms did not change, the daf-16 loss-of-function mutant with an extremely short lifespan showed much variation when chemically synchronized. This study clarifies the impacts of chemical synchronization of worms and provides some critical considerations for its use.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"20-37"},"PeriodicalIF":3.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13637758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria-ER contact sites in neurological disorders. 神经系统疾病中的线粒体-内质网接触部位。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-09-21 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2725542

Eun Jin Jang, Jisoo Lee, Seungha Shin, Tran Diem Nghi, Sang Ki Park

引用次数: 0

Circuit-specific remodeling of synaptic co-transmission in stress-responsive networks. 应激反应网络中突触共传递的电路特异性重构。

IF 3.6 2区生物学

Animal Cells and Systems Pub Date : 2026-09-18 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2026.2730204

Hoyong Park, Hyogyun Kim, Minsung Sim, Doyeon Ham, ChiHye Chung

{"title":"Circuit-specific remodeling of synaptic co-transmission in stress-responsive networks.","authors":"Hoyong Park, Hyogyun Kim, Minsung Sim, Doyeon Ham, ChiHye Chung","doi":"10.1080/19768354.2026.2730204","DOIUrl":"10.1080/19768354.2026.2730204","url":null,"abstract":"Neurons do not always use a 'one neurotransmitter-one receptor' paradigm to communicate at synapses. Some neurons communicate using more than one neurotransmitter, a phenomenon termed co-transmission. Co-transmission can occur through a few distinct organizational modes at synapses, enabling the integration of excitatory, inhibitory, and modulatory signals in various combinations. Increasingly, evidence suggests that these organizational modes are particularly important in neural circuits functioning in stress response. In monoaminergic nuclei, the lateral habenula, and the hippocampus, stress exposure induces shifts in co-transmission via circuit-specific changes to neurotransmitter balance. Here, we review recent advances in our understanding of co-transmission in these circuits, with an emphasis on evidence that distinct modes of small-molecule co-transmission are employed in circuits that contribute to some of the multidimensional aspects of stress. We further discuss potential mechanisms that may recalibrate multi-transmitter signaling during stress-stimulated co-transmission. Currently, evidence suggests that stress affects not only single-transmitter systems but also multi-transmitter communication across neural circuits. Viewing co-transmission as a dynamic substrate of stress-induced neural plasticity, this review provides new insight into the neural basis of adaptive and maladaptive stress responses and may help identify novel therapeutic opportunities for affective disorders, such as major depressive disorder and bipolar disorder.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"39-57"},"PeriodicalIF":3.6,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13591946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0