Animal Cells and Systems最新文献

AP001885.4 promotes the proliferation of esophageal squamous cell carcinoma cells by histone lactylation- and NF-κB (p65)-dependent transcription activation and METTL3-mediated mRNA stability of c-myc. AP001885.4 通过组蛋白乳化和 NF-κB (p65) 依赖性转录激活以及 METTL3 介导的 c-myc mRNA 稳定促进食管鳞状细胞癌细胞的增殖。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2417458

Chuang Fu, Wen Jiang, Chong Wang, Sheng-Jie Song, Hao Tao, Xin-Guo Zhang, Wen-Ting Li, Xin Jin, Bin-Bing Yu, Jia-Jie Hao, Wen-Juan Sun, Jie Bai, Zhi-Zhou Shi

{"title":"AP001885.4 promotes the proliferation of esophageal squamous cell carcinoma cells by histone lactylation- and NF-κB (p65)-dependent transcription activation and METTL3-mediated mRNA stability of c-myc.","authors":"Chuang Fu, Wen Jiang, Chong Wang, Sheng-Jie Song, Hao Tao, Xin-Guo Zhang, Wen-Ting Li, Xin Jin, Bin-Bing Yu, Jia-Jie Hao, Wen-Juan Sun, Jie Bai, Zhi-Zhou Shi","doi":"10.1080/19768354.2024.2417458","DOIUrl":"10.1080/19768354.2024.2417458","url":null,"abstract":"Esophageal squamous cell carcinoma (ESCC) is an aggressive malignant neoplasm, and up to now, the role of long non-coding RNA (lncRNA) AP001885.4 in cancer, including ESCC, is absolutely unclear. The GEPIA database was applied to identify differentially expressed and prognosis-associated genes in esophageal cancer (ESCA). CCK-8, colony formation, Western blot, and qRT-PCR methods were harnessed to investigate the role and mechanism of AP001885.4 in esophageal carcinogenesis. By analyzing TCGA data in the GEPIA database, two lncRNAs were selected. AP001885.4 was overexpressed and positively associated with the unfavorable outcome of ESCC patients, and LINC001786 was under-expressed and negatively linked with the poor prognosis. Knockdown of AP001885.4 suppressed the proliferation and colony formation of ESCC cells. Importantly, the silence of AP001885.4 downregulated c-myc. Mechanically, the knockdown of AP001885.4 reduced METTL3 expression and m6A modification in c-myc mRNA, and METTL3 positively regulated c-myc. Furthermore, the knockdown of AP001885.4 diminished histone lactylation and NF-κB (p65) expression, and the protein lactylation inhibitors (2-DG, 2-deoxy-D-glucose and oxamate) and the NF-κB inhibitor (JSH-23) also lessened c-myc expression. Consequently, our findings suggested that AP001885.4 promoted the proliferation of esophageal squamous cell carcinoma cells by histone lactylation- and NF-κB (p65)-dependent transcription activation and METTL3-mediated mRNA stability of c-myc.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"536-550"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative transcriptomic profiling uncovers immune and functional responses to bisphenol a across multiple tissues in male mice. 综合转录组分析揭示了雄性小鼠多个组织对双酚 A 的免疫和功能反应。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-10-24 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2419473

Yejee Park, Min-Jae Jang, Do-Yeal Ryu, Byeonghwi Lim, Rajesh Kumar Pathak, Myung-Geol Pang, Jun-Mo Kim

{"title":"Integrative transcriptomic profiling uncovers immune and functional responses to bisphenol a across multiple tissues in male mice.","authors":"Yejee Park, Min-Jae Jang, Do-Yeal Ryu, Byeonghwi Lim, Rajesh Kumar Pathak, Myung-Geol Pang, Jun-Mo Kim","doi":"10.1080/19768354.2024.2419473","DOIUrl":"10.1080/19768354.2024.2419473","url":null,"abstract":"Bisphenol A (BPA), an endocrine-disrupting substance commonly found in plastics and receipts, is associated with adverse effects, including endocrine disorders, reduced fertility, and metabolic issues. To gain insights into its effects on biological systems, we observed the adverse effects of BPA in male Institute of Cancer Research (ICR) mice exposed to BPA at the lowest observed adverse effect level for 6 weeks, in comparison with the control groups. We constructed a comprehensive transcriptome profile using 20 different tissues to analyze the changes in the whole-body systems. This involved employing differential gene expression, tissue-specific gene, and gene co-expression network analyses. The study revealed that BPA exposure led to significant differences in the transcriptome in the thymus, suggesting activation of T-cell differentiation and maturation in response to BPA treatment. Furthermore, various tissues exhibited immune response activation, potentially due to the migration of immune cells from the thymus. BPA exposure also caused immune-related functional changes in the colon, liver, and kidney, as well as abnormal signaling responses in the sperm. The transcriptome analysis serves as a valuable resource for understanding the functional impact of BPA, providing profound insights into the effects of BPA exposure and emphasizing the need for further research on potential associated health risks.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"519-535"},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11504166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ank-mediated pyrophosphate regulates shear stress-induced small extracellular vesicle production in 3D-cultured osteocytes. Ank 介导的焦磷酸调节剪切应力诱导的三维培养成骨细胞小细胞外囊泡的产生。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2409460

Su Jeong Lee, Deuk Kju Jung, Soomin Im, Changkook You, Jung-Eun Kim, Jong-Sup Bae, Mee-Seon Kim, Kyungmoo Yea, Eui Kyun Park

{"title":"Ank-mediated pyrophosphate regulates shear stress-induced small extracellular vesicle production in 3D-cultured osteocytes.","authors":"Su Jeong Lee, Deuk Kju Jung, Soomin Im, Changkook You, Jung-Eun Kim, Jong-Sup Bae, Mee-Seon Kim, Kyungmoo Yea, Eui Kyun Park","doi":"10.1080/19768354.2024.2409460","DOIUrl":"https://doi.org/10.1080/19768354.2024.2409460","url":null,"abstract":"Osteocytes are located in the lacunae of fluid-filled bone and communicate with neighboring or distant cells by secreting small extracellular vesicles (sEVs) and growth factors as well as via dendrite-dendrite direct connections. However, the mechanism regulating sEV production in osteocytes is yet to be elucidated. In this study, we investigated sEV production and its underlying mechanism in osteocytes cultured on a three dimensional (3D) scaffold. We employed a perfusion system to apply shear stress stimulation to MLO-Y4 cells cultured on a 3D biphasic calcium phosphate (BCP) scaffold and analyzed sEV production and gene expression using RNA sequencing. We found that the expression of genes associated with sEV biogenesis and the secretory pathway were enhanced by fluid shear stress in MLO-Y4 cells cultured on a 3D BCP scaffold. In particular, fluid shear stress induced the expression of Ank, a pyrophosphate transporter, in 3D-cultured MLO-Y4 cells. The role of Ank in sEV production was further examined. Probenecid, an Ank inhibitor, significantly suppressed shear stress-induced sEV production, whereas Ank cDNA overexpression stimulated it. The inhibition of shear stress-induced sEV production by probenecid was recovered by the exogenous addition of pyrophosphate to MLO-Y4 cells. These findings suggest that shear stress-mediated sEV production in 3D-cultured osteocytes is regulated by extracellular pyrophosphate transported by Ank.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"495-505"},"PeriodicalIF":2.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging roles of cytoskeletal transport and scaffold systems in human viral propagation. 细胞骨架运输和支架系统在人类病毒传播中的新作用。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2418332

Younghyun Lim, Yong-Bin Cho, Young-Jin Seo

{"title":"Emerging roles of cytoskeletal transport and scaffold systems in human viral propagation.","authors":"Younghyun Lim, Yong-Bin Cho, Young-Jin Seo","doi":"10.1080/19768354.2024.2418332","DOIUrl":"https://doi.org/10.1080/19768354.2024.2418332","url":null,"abstract":"Viruses have long been recognized as significant pathogens, contributing to multiple global pandemics throughout human history. Recent examples include the 2009 influenza pandemic and the COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019. Despite ongoing experimental and clinical efforts, the development of effective antiviral treatments and vaccines remains challenging due to the high mutation rates of many human pathogenic viruses including influenza virus and SARS-CoV-2. As an alternative approach, antiviral strategies targeting host factors shared by multiple viruses could provide a more universally applicable solution. Emerging evidence suggests that viruses exploit the host cytoskeletal network to facilitate efficient viral replication and propagation. Therefore, a comprehensive understanding of the interactions between viral components and the cytoskeletal machinery may offer valuable insights for the development of broad-spectrum antiviral therapeutics. This review compiles and discusses current knowledge on the interactions between viruses and cytoskeletal elements, including kinesin, dynein, myosin, and vimentin, and explores their potential as therapeutic targets. The potential for these cytoskeletal components to serve as targets for new antiviral interventions is discussed in the context of diverse human viruses, including influenza virus, SARS-CoV-2, herpes simplex virus, human papillomavirus, and human immunodeficiency virus.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"506-518"},"PeriodicalIF":2.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11494721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen-doped carbon quantum dot regulates cell proliferation and differentiation by endoplasmic reticulum stress. 掺氮碳量子点通过内质网应激调节细胞增殖和分化

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-10-02 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2409452

Hyun Hee Song, Hyunwoo Choi, Seonghan Kim, Hwan Gyu Kim, Sangmin An, Sejung Kim, Hoon Jang

{"title":"Nitrogen-doped carbon quantum dot regulates cell proliferation and differentiation by endoplasmic reticulum stress.","authors":"Hyun Hee Song, Hyunwoo Choi, Seonghan Kim, Hwan Gyu Kim, Sangmin An, Sejung Kim, Hoon Jang","doi":"10.1080/19768354.2024.2409452","DOIUrl":"10.1080/19768354.2024.2409452","url":null,"abstract":"Quantum dots have diverse biomedical applications, from constructing biological infrastructures like medical imaging to advancing pharmaceutical research. However, concerns about human health arise due to the toxic potential of quantum dots based on heavy metals. Therefore, research on quantum dots has predominantly focused on oxidative stress, cell death, and other broader bodily toxicities. This study investigated the toxicity and cellular responses of mouse embryonic stem cells (mESCs) and mouse adult stem cells (mASCs) to nitrogen-doped carbon quantum dots (NCQDs) made of non-metallic materials. Cells were exposed to NCQDs, and we utilized a fluorescent ubiquitination-based cell system to verify whether NCQDs induce cytotoxicity. Furthermore, we validated the differentiation-inducing impact of NCQDs by utilizing embryonic stem cells equipped with the Oct4 enhancer-GFP reporter system. By analyzing gene expression including Crebzf, Chop, and ATF6, we also observed that NCQDs robustly elicited endoplasmic reticulum (ER) stress. We confirmed that NCQDs induced cytotoxicity and abnormal differentiation. Interestingly, we also confirmed that low concentrations of NCQDs stimulated cell proliferation in both mESCs and mASCs. In conclusion, NCQDs modulate cell death, proliferation, and differentiation in a concentration-dependent manner. Indiscriminate biological applications of NCQDs have the potential to cause cancer development by affecting normal cell division or to fail to induce normal differentiation by affecting embryonic development during pregnancy. Therefore, we propose that future biomedical applications of NCQDs necessitate comprehensive and diverse biological studies.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"481-494"},"PeriodicalIF":2.5,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11448352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender-specific alteration of steroid metabolism and its impact on viral replication in a mouse model of hepatitis B virus infection. 乙型肝炎病毒感染小鼠模型中类固醇代谢的性别特异性改变及其对病毒复制的影响。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-09-17 DOI: 10.1080/19768354.2024.2403569

Eun-Sook Park,Juhee Won,Sung Hyun Ahn,Ah Ram Lee,Donghyo Lee,Ju-Yeon Moon,Man Ho Choi,Kyun-Hwan Kim

{"title":"Gender-specific alteration of steroid metabolism and its impact on viral replication in a mouse model of hepatitis B virus infection.","authors":"Eun-Sook Park,Juhee Won,Sung Hyun Ahn,Ah Ram Lee,Donghyo Lee,Ju-Yeon Moon,Man Ho Choi,Kyun-Hwan Kim","doi":"10.1080/19768354.2024.2403569","DOIUrl":"https://doi.org/10.1080/19768354.2024.2403569","url":null,"abstract":"Hepatitis B virus (HBV) is a sex-specific pathogen that is more severe in males than in females. Sex disparities in HBV infection have been attributed to hormonal differences between males and females. However, whether HBV infection affects the metabolic signatures of steroid hormones and how these influences viral replication remains unclear. In this study, we investigated whether HBV infection alters steroid metabolism and its effects on HBV replication. Serum samples from male and female mice obtained after the hydrodynamic injection of replication-competent HBV plasmids were subjected to quantitative steroid profiling. Serum steroid levels in mice were analyzed using an in vitro metabolism assay with the mouse liver S9 fraction. The alteration of steroids by HBV infection was observed only in male mice, particularly with significant changes in androgens, whereas no significant hormonal changes were observed in female mice. Among the altered steroids, dehydroepiandrosterone (DHEA) levels increased the most in male mice after HBV infection. An in vitro metabolism assay revealed that androgen levels were significantly reduced in HBV-infected male mice. Furthermore, the genes involved in DHEA biosynthesis were significantly upregulated in HBV-infected male mice. Interestingly, reduced dihydrotestosterone in male mice significantly inhibits viral replication by suppressing HBV promoter activity, suggesting a viral strategy to overcome the antiviral effects of steroid hormones in males. Our data demonstrated that HBV infection can cause sex-specific changes in steroid metabolism.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"35 1","pages":"466-480"},"PeriodicalIF":2.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of maternal nonylphenol exposure on the proliferation of glial cells in the brain of male offspring mice. 母体接触壬基酚对雄性后代小鼠大脑胶质细胞增殖的影响

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-09-12 DOI: 10.1080/19768354.2024.2401389

Seung Hyun Lee,Hyun Seung Shin,Yun Hee So,Dong Hun Lee,Jin Yeop Kim,Eun-Hee Lee,Eui-Man Jung

{"title":"Effects of maternal nonylphenol exposure on the proliferation of glial cells in the brain of male offspring mice.","authors":"Seung Hyun Lee,Hyun Seung Shin,Yun Hee So,Dong Hun Lee,Jin Yeop Kim,Eun-Hee Lee,Eui-Man Jung","doi":"10.1080/19768354.2024.2401389","DOIUrl":"https://doi.org/10.1080/19768354.2024.2401389","url":null,"abstract":"Glial cells play a significant role in maintaining brain homeostasis and normal brain development, and their functions can be impaired by exposure to endocrine disruptors. 4-n-Nonylphenol (NP), a representative endocrine disruptor, is widely used in personal care products and industrial materials. NP accumulates in various organs, including the brain, of living organisms and adversely influences brain health. However, studies on the effects of NP on glial cells are limited. This study aims to investigate the effects of NP on glial cells using primary mixed glial cells and offspring mice exposed to NP during gestation and lactation. In vitro experiments revealed that NP exposure stimulated the astrocytes and microglia proliferation but not oligodendrocytes. NP exposure activated microglia and reduced myelin protein expression in oligodendrocytes. Moreover, maternal NP exposure increased the numbers of microglia and oligodendrocytes in the cerebral cortex of adult offspring. NP exposure caused anxiety- and depressive-like behaviors in adult mice. Collectively, these findings suggest that maternal NP exposure negatively affects the brain development in adult offspring mice.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"439-452"},"PeriodicalIF":2.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CXCL10 promotes melanoma angiogenesis and tumor growth CXCL10 促进黑色素瘤血管生成和肿瘤生长

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-09-11 DOI: 10.1080/19768354.2024.2402024

Bongjun Kim, Yun-Yong Park, Jong-Ho Lee

引用次数: 0

Low-temperature pulverization-specific Sargassum horneri extract accelerates wound healing and attenuates inflammation in a mouse burn model. 低温粉碎特异性马尾藻提取物可加速小鼠烧伤模型的伤口愈合并减轻炎症反应。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-09-05 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2396903

Eunguk Shin, Hee-Tae Kim, Haksoo Lee, Byeongsoo Kim, Junhyeong Park, Sujin Park, Soomin Yum, Seul-Kee Kim, Jae-Myung Lee, BuHyun Youn

{"title":"Low-temperature pulverization-specific Sargassum horneri extract accelerates wound healing and attenuates inflammation in a mouse burn model.","authors":"Eunguk Shin, Hee-Tae Kim, Haksoo Lee, Byeongsoo Kim, Junhyeong Park, Sujin Park, Soomin Yum, Seul-Kee Kim, Jae-Myung Lee, BuHyun Youn","doi":"10.1080/19768354.2024.2396903","DOIUrl":"10.1080/19768354.2024.2396903","url":null,"abstract":"Burn injuries, affecting local skin disruption as well as inducing systemic inflammatory responses, are presented as a global public health problem. To enhance the effects of burn wound healing, treatment must simultaneously regulate both re-epithelialization and hyperinflammation. Extracts of Sargassum horneri (S. horneri) have shown a potential to enhance skin wound healing through antioxidative properties, immune enhancement, and modulation of inflammatory responses. However, despite its promising application for burn wound healing, specific investigation into S. horneri-derived compounds for enhancing wound healing has not yet been conducted. In this research, we investigated the burn wound-healing effect of the low-temperature pulverization-specific S. horneri extract (LPSHE), which could not be detected using the room-temperature grinding method. In a mouse burn model with third-degree burn injuries, LPSHE accelerated re-epithelialization by promoting the increase in F-actin formation and reduced burn-induced ROS levels. Additionally, LPSHE significantly regulated hyperinflammation by reducing pro-inflammatory cytokines. Further investigation into molecular mechanisms using HaCaT keratinocytes also demonstrated beneficial effects on burn wound healing. Taken together, our findings suggested that LPSHE is a promising therapeutic candidate for enhancing burn wound healing. Furthermore, this research underscored the importance of low-temperature pulverization in discovering novel natural compounds from marine organisms.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"428-438"},"PeriodicalIF":2.5,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ECM stiffness regulates calcium influx into mitochondria via tubulin and VDAC1 activity. ECM 硬度通过微管蛋白和 VDAC1 的活性调节线粒体的钙离子流入。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-08-29 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2393811

Minji Kim, Kiseok Han, Gyuho Choi, Sanghyun Ahn, Jung-Soo Suh, Tae-Jin Kim

{"title":"ECM stiffness regulates calcium influx into mitochondria via tubulin and VDAC1 activity.","authors":"Minji Kim, Kiseok Han, Gyuho Choi, Sanghyun Ahn, Jung-Soo Suh, Tae-Jin Kim","doi":"10.1080/19768354.2024.2393811","DOIUrl":"10.1080/19768354.2024.2393811","url":null,"abstract":"Calcium ions (Ca2+) play pivotal roles in regulating numerous cellular functions, including metabolism and growth, in normal and cancerous cells. Consequently, Ca2+ signaling is a vital determinant of cell fate and influences both cell survival and death. These intracellular signals are susceptible to modulation by various factors, including changes in the extracellular environment, which leads to mechanical alterations. However, the effect of extracellular matrix (ECM) stiffness variations on intracellular Ca2+ signaling remains underexplored. In this study, we aimed to elucidate the mechanisms of Ca2+ regulation through the mitochondria, which are crucial to Ca2+ homeostasis. We investigated how Ca2+ regulatory mechanisms adapt to different levels of ECM stiffness by simultaneously imaging the mitochondria and endoplasmic reticulum (ER) in live cells using genetically encoded biosensors. Our findings revealed that the uptake of mitochondrial Ca2+ through Voltage-Dependent Anion Channel 1 (VDAC1), facilitated by intracellular tubulin, is influenced by ECM stiffness. Unraveling these Ca2+ regulatory mechanisms under various conditions offers a novel perspective for advancing biomedical studies involving Ca2+ signaling.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"28 1","pages":"417-427"},"PeriodicalIF":2.5,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11363740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0