Animal Cells and Systems最新文献

{"title":"Calcium hydroxyapatite suppresses extracellular matrix-related gene expression by modulating Piezo1-associated Ca²⁺ signaling and PKA activity.","authors":"ChanHui Song, Younjee Jeong, Tae-Jin Kim","doi":"10.1080/19768354.2026.2677272","DOIUrl":"10.1080/19768354.2026.2677272","url":null,"abstract":"<p><p>Fibrosis is characterized by excessive extracellular matrix (ECM) deposition driven by sustained pro-fibrotic signaling and underlies a wide range of pathological conditions. Accordingly, strategies that broadly suppress ECM production are considered promising for the treatment of fibrosis. Although calcium hydroxyapatite (CaHA) is primarily used as a nanoparticle-based dermal filler, previous observations that it affects collagen production suggest its potential repurposing as a regulator of ECM-related programs. Here, we investigated the anti-fibrotic efficacy of CaHA and the signaling mechanisms underlying its effects. CaHA broadly suppressed ECM-related gene expression in fibroblasts, extending beyond collagen to multiple ECM components and associated regulatory factors. Mechanistically, CaHA attenuated Piezo1-associated Ca²⁺ signaling and was also associated with activation of the PKA pathway. Importantly, CaHA similarly reduced ECM-related gene expression in a fibrotic liver cell model, supporting the broader relevance of its anti-fibrotic activity. Together, these findings identify CaHA as a broad suppressor of ECM-related gene expression through modulation of Piezo1-associated Ca²⁺ signaling and PKA-associated pathways, and support its potential as an anti-fibrotic therapeutic strategy.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"459-470"},"PeriodicalIF":3.6,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13463449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo whole transcriptome sequencing reveals differentially expressed messenger RNA and MicroRNA in hepatopancreas of <i>Pandalus hypsinotus</i>.","authors":"Du Hyeong Lee, Woo Ryung Kim, Hae Jin Shin, Jung-Min Kim, Eun Gyung Park, Yun Ju Lee, Hyeon-Su Jeong, Hyun-Young Roh, Yung Hyun Choi, In-Tae Cha, Heui-Soo Kim","doi":"10.1080/19768354.2026.2692155","DOIUrl":"10.1080/19768354.2026.2692155","url":null,"abstract":"<p><p><i>Pandalus hypsinotus (P. hypsinotus)</i>, also known as coonstripe shrimp, has high economic value and is widely distributed in Alaska, the Bering Sea and the East Sea of Korea. However, owing to the low catch rates and patchy spatial distribution, transcriptome-based studies on this species remain limited. Transcriptome analyses using PacBio Iso-Seq and Illumina RNA sequencing techniques were performed to establish a de novo unigene set of <i>P. hypsinotus</i> and identify differentially expressed genes (DEGs) in the hepatopancreas compared to the tail muscle. A search of all the unigenes against Gene Ontology (GO), KEGG, Pfam, BLASTN, and UniProt resulted in 46,703 annotated unigenes. A comparison of the sequences in the two tissue-derived libraries revealed 9,429 DEGs in the hepatopancreas. We also investigated the small RNAomes of <i>P. hypsinotus</i> using the Illumina small RNA sequencing technology. Because miRNAs are highly sensitive to stress responses and pathogen infections, they serve as effective indicators of the immune status, thereby acting as crucial tools for enhancing productivity and disease control in the aquaculture industry. The 130 identified microRNA (miRNA) candidates were classified into 32 orthologous and 98 novel miRNAs using BLASTN and miRBase. Through GO analysis and direct interactions between miRNAs and genes, the differentially expressed miRNAs (DEMs) and DEGs related to immune responses showed regulatory relationships based on their sequences. These findings provide a valuable resource for future studies on novel gene detection, gene mapping and biological pathways that may be regulated by miRNAs unique to <i>P. hypsinotus</i>.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"419-444"},"PeriodicalIF":3.6,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13435287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148672715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD71 defines functionally active spermatogonial stem cells with enhanced transplantation potential in mouse testes.","authors":"Beom-Jin Shin, Jin Seop Ahn, Jeeseung Kim, Jeongho Lee, Soontag Jung, Sang-Eun Jung, Hyo Jin Gu, Sung-Hwan Moon, Seung Hee Shin, Hyun Min Lee, Changsun Choi, Buom-Yong Ryu, Chun Jeih Ryu","doi":"10.1080/19768354.2026.2709175","DOIUrl":"10.1080/19768354.2026.2709175","url":null,"abstract":"<p><p>Spermatogonial stem cells (SSCs) are responsible for lifelong spermatogenesis in adult males; however, their scarcity and inherent heterogeneity, coupled with the lack of robust SSC-specific surface markers, continue to impede isolation and characterization. In the present study, we found CD71, which corresponds to transferrin receptor (TfR1; encoded by <i>Tfrc</i>), a candidate marker capable of enriching for SSC populations from mouse testes. The immunohistochemistry detected co-localization of CD71 with the undifferentiated spermatogonia marker glial cell line-derived neurotrophic factor family receptor alpha 1 (GFRα1) on the seminiferous basement membrane, with approximately 81% co-localization. Consistent with this finding, the expression of <i>Tfrc</i> was up-regulated in SSC-enriched germ cell populations relative to mouse germ cell lines (GC-1 and GC-2) and somatic cell lines from testis (TM3 and TM4). Fluorescence-activated cell sorting (FACS) analysis further showed that GFRα1⁺ cells exhibited approximately 1.4-fold higher <i>Tfrc</i> mRNA expression than GFRα1^- cells. Similarly, CD71⁺ cells exhibited significantly higher expression of the undifferentiated spermatogonia markers <i>Id4</i>, <i>Lhx1</i>, <i>Gfrα1</i>, <i>Zbtb16</i>, and <i>Etv5</i>. Functional transplantation assays further demonstrate that CD71^high cells give rise to approximately 5.5-fold more colonies than freshy isolated, FACS-unsorted donor cells. Moreover, peanut agglutinin (PNA) lectin staining confirmed the normal spermatogenic differentiation within colonies derived from CD71^high donor cells. Our findings collectively indicate that CD71^high cells represent an SSC-enriched population with enhanced spermatogenic regenerative capacity and support the use of CD71 as a complementary marker for SSC enrichment and fertility restoration.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"448-461"},"PeriodicalIF":3.6,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13425539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cathepsin A deficiency exacerbates LPS-induced inflammatory liver injury and apoptosis.","authors":"Hee Young Chae, Ji Yeong Park, Hyejin Hyung, Jiwon Ko, Su-Geun Lim, Young Jin Lee, Myoung Ok Kim, Song Park, Dong Kyu Choi, Soyoung Jang, Zae Young Ryoo, Soyeon Jang","doi":"10.1080/19768354.2026.2671489","DOIUrl":"10.1080/19768354.2026.2671489","url":null,"abstract":"<p><p>Hepatic inflammation plays a key role in acute and chronic liver diseases by driving hepatocellular injury and promoting disease progression. However, the molecular mechanisms that maintain hepatic immune homeostasis remain unclear. Cathepsin A (<i>Ctsa</i>), a lysosomal serine carboxypeptidase involved in protein degradation and enzyme stabilization, has been implicated in lysosomal storage disorders. However, its role in liver immunity is poorly understood. Given the emerging evidence that lysosomal proteases contribute to immune regulation and inflammatory signaling, <i>Ctsa</i> is a promising yet underexplored candidate for elucidating how lysosomal proteases influence hepatic inflammation. To address this gap, this study aimed to investigate <i>Ctsa</i> function using <i>Ctsa</i> knockout (<i>Ctsa</i> ^-/-) mice. Under basal conditions, <i>Ctsa</i> ^-/- mice exhibited splenic immune activation and relative hepatomegaly accompanied by histological alterations. Following lipopolysaccharide challenge, the mice developed aggravated liver injury with elevated aminotransferase levels, enhanced immune cell infiltration, and increased pro-inflammatory cytokine expression. These inflammatory changes were accompanied by increased hepatocellular apoptosis, as evidenced by elevated Bax/Bcl-2 ratio, cleaved caspase-3 expression, and increased TUNEL-positive cells. Collectively, these findings indicate that <i>Ctsa</i> contributes to the regulation of hepatic immune and cellular homeostasis, and its loss increases susceptibility to inflammatory liver injury.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"406-418"},"PeriodicalIF":3.6,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13185071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147980166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD47-mediated purification of human ventricular cardiomyocytes derived from pluripotent stem cells.","authors":"Soon-Jung Park, Hyung Kyu Choi, Keon Ha Park, Na Kyeong Park, Sung-Hwan Moon","doi":"10.1080/19768354.2026.2657637","DOIUrl":"10.1080/19768354.2026.2657637","url":null,"abstract":"<p><p>Derivation of electrophysiologically mature cardiomyocytes from human pluripotent stem cells (hPSCs) remains a prerequisite for effective cardiac modeling and preclinical drug evaluation. However, current differentiation protocols often produce heterogeneous cell populations with limited maturity. In this study, we developed a CD47-based fluorescence-activated cell sorting strategy to purify ventricular-like cardiomyocytes from three-dimensionally differentiated hPSC-derived spheroids. CD47⁺ cardiomyocytes exhibited highly consistent contractile behavior and enhanced structural maturation, as confirmed using immunocytochemistry for myosin regulatory light chain 2 ventricular/cardiac muscle isoform and α-actinin. Electrophysiological analysis using a patch clamp revealed that the majority of CD47⁺ cells displayed ventricular-like action potential waveforms, characterized by prolonged repolarization duration, elevated amplitude, and a reduced negative maximum diastolic potential. Drug responsiveness was assessed using multi-electrode array recordings. CD47-enriched cardiomyocytes demonstrated reproducible and dose-dependent field potential alterations in response to known cardiotoxic agents, including remdesivir and quinidine, and outperformed metabolically purified controls in sensitivity and inter-replicate consistency analysis. These results establish CD47 as a reliable surface marker for isolating mature ventricular-like cardiomyocytes from hPSCs. The method enables the generation of functionally robust cardiomyocyte populations suitable for <i>in vitro</i> cardiac research and pharmacological testing.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"381-393"},"PeriodicalIF":3.6,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influences of pupal presence and worker number on the survival and development of pharaoh ant (<i>Monomorium pharaonis</i>) brood under laboratory conditions.","authors":"Hee Jin Baek, Byunghyuk Kim","doi":"10.1080/19768354.2026.2657636","DOIUrl":"10.1080/19768354.2026.2657636","url":null,"abstract":"<p><p>Social isolation and inadequate group composition can severely impair brood survival in eusocial insects due to the loss of social interactions. Establishing small artificial colonies is a prerequisite for studying developmental biology and functional genetics in ants; however, the minimal social requirements for effective brood rearing remain poorly understood. In this study, we investigated the impact of social composition - specifically the presence of pupae and worker abundance - on the survival and development of intact eggs within laboratory-maintained sub-colonies of the pharaoh ant, <i>Monomorium pharaonis</i>. Our analysis revealed that supplementing colonies with pupae significantly enhanced brood survival and accelerated larval development. Furthermore, we identified that sufficient worker numbers improve brood success, supporting the existence of a viable colony size. Our data suggest that while a sufficient workforce is essential for brood care, further increases in worker number do not yield proportional gains in rearing efficiency under our laboratory conditions. These results indicate that establishing favorable rearing conditions depends on achieving a functional demographic balance, particularly through pupal supplementation and an appropriate worker ratio. This study highlights the vital role of colony demographics and pupal presence in ant development, providing a practical framework for brood manipulation and ensuring baseline survival for future functional genetic applications.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"394-405"},"PeriodicalIF":3.6,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147760080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manic switching from depression is affected by adult hippocampal neurogenesis.","authors":"Jieun Song, Woonryong Kim, Yoojin Park, Ja-Myong Lee, Jong-Hoon Kim, Dong-Hyuk Park, Heon-Jeong Lee, Ki-Soon Shin, Hyun Jung Kim, Dongho Geum","doi":"10.1080/19768354.2026.2654260","DOIUrl":"10.1080/19768354.2026.2654260","url":null,"abstract":"<p><p>Many individuals who initially present with depression later develop manic or hypomanic episodes, yet the biological processes accompanying this delayed shift remain unclear. Here, we examined whether perturbation of the adult hippocampal neurogenic niche is associated with time-dependent shifts in mood-relevant behaviors. Using Nestin-CreER::Rosa26-FloxedSTOP-DTa (Nestin-DTa) mice, we induced partial ablation of Nestin-positive adult neural stem cells (aNSCs) in the hippocampus by tamoxifen administration and assessed phenotypes at 4 and 8 weeks post-ablation. Neurogenic-lineage populations in the dentate gyrus were quantified by immunohistochemistry using stage-specific markers. Partial aNSC ablation reduced the Nestin-positive pool and BrdU-labeled proliferation, followed by a compensatory expansion of proliferative progenitors and DCX-positive neuroblasts. At 4 weeks, Nestin-DTa mice exhibited anxiety-like behavior and increased forced-swim immobility. By 8 weeks, anxiety-like measures normalized, and mice displayed increased exploratory/locomotor activity and reduced forced-swim immobility, accompanied by sustained elevation of progenitor and immature neuronal compartments. These findings provide a tractable model in which disruption of the adult neurogenic niche is associated with a transition from depression-like behavior to a hyperactive, mania-like phenotype over time.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"368-380"},"PeriodicalIF":3.6,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and metabolic dysfunction in oral lichen planus pathogenesis.","authors":"Hyo-Jin Song, Kun-Hwa Kang, Jin-Seok Byun, Do-Yeon Kim","doi":"10.1080/19768354.2026.2648907","DOIUrl":"10.1080/19768354.2026.2648907","url":null,"abstract":"<p><p>Oral lichen planus (OLP) is a chronic, immune-mediated inflammatory disease of the oral mucosa that can cause persistent pain and has a recognized, though relatively low, risk of malignant transformation. Recent studies have identified oxidative and nitrosative stress as key contributors to the pathophysiology of OLP. Excessive production of reactive oxygen and nitrogen species, combined with reduced antioxidant defenses, leads to cellular damage, including lipid peroxidation, protein modification, and DNA injury. This oxidative imbalance drives keratinocyte apoptosis and sustains inflammatory signaling, creating a pro-inflammatory tissue environment. In parallel, metabolic dysfunction is increasingly recognized in OLP, characterized by altered energy production and nutrient utilization that disrupt tissue homeostasis. These two processes are closely linked, as oxidative stress impairs metabolic pathways, while metabolic alterations further increase oxidative burden, forming a self-reinforcing cycle that perpetuates mucosal injury and chronic inflammation. Clinically, markers of oxidative stress and metabolic imbalance, particularly those detectable in saliva, show promise for non-invasive diagnosis and risk assessment. Therapeutic strategies aimed at restoring redox balance and improving metabolic regulation represent emerging avenues beyond conventional symptom management. Understanding the crosstalk between oxidative stress and metabolism offers new opportunities for precision medicine approaches to improve the diagnosis, treatment, and long-term outcomes of patients with OLP.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"353-367"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancer RNA-mediated transcriptional regulation of TDP-43 during early neural lineage specification.","authors":"Yeonju Jang, Hyohi Lee, Myunggeun Oh, Jiin Moon, Seung-Jin Kim, Jaeil Han, Jong-Lyul Park, Seung-Kyoon Kim","doi":"10.1080/19768354.2026.2643998","DOIUrl":"10.1080/19768354.2026.2643998","url":null,"abstract":"<p><p>TAR DNA-binding protein 43 (TDP-43) is a DNA- and RNA-binding protein that regulates gene expression by modulating transcription and RNA processing. It plays pivotal roles in neuronal development and function, and its mislocalization and aggregation are major pathological features of several neurodegenerative diseases. However, the regulatory mechanisms that control <i>Tdp-43</i> expression and activity during the transition from embryonic stem cells (ESCs) to neural progenitor cells (NPCs) remain poorly understood. Through integrative epigenomic and transcriptomic analyses, we identified multiple intergenic and intragenic enhancers within and around the <i>Tdp-43</i> locus that generate enhancer RNAs (eRNAs). These eRNAs exhibit dynamic, region-specific expression changes and modulate <i>Tdp-43</i> transcription in a stage- and context-dependent manner. Specifically, a subset of eRNAs was highly expressed in ESCs and downregulated upon differentiation, while others were selectively retained or induced in NPCs, paralleling changes in enhancer usage and histone modification states. Targeted knockdown of these eRNAs decreased <i>Tdp-43</i> expression and was accompanied by changes in the expression of pluripotency- and lineage-associated markers, without implying direct control over full differentiation trajectories. These findings uncover a previously unrecognized aspect of <i>Tdp-43</i> transcriptional regulation and highlight the significance of enhancer dynamics in the epigenetic regulation of TDP-43 expression during early lineage specification.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"320-341"},"PeriodicalIF":3.6,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative phylotranscriptomics reveals glycolytic adaptation associated with prolonged larval development in the coastal slipper lobster <i>Crenarctus bicuspidatus</i>.","authors":"Hyeongwoo Choi, Hyeongju Choi, Chungkug Park, Won-Kyo Jung, Tae-Hoon Kim, Yun Keun An","doi":"10.1080/19768354.2026.2643995","DOIUrl":"10.1080/19768354.2026.2643995","url":null,"abstract":"<p><p>The molecular basis of ecological adaptation in marine crustaceans remains poorly understood, particularly in non-model species such as the coastal slipper lobster (<i>Crenarctus bicuspidatus</i>). This study presents the first comprehensive analysis of the mitochondrial genome and transcriptome of <i>C. bicuspidatus</i> using high-throughput sequencing. Comparative transcriptomic analysis across 13 crustacean species identified 250 single-copy orthogroups. Gene family evolution analysis revealed 28 gene family expansions and 152 contractions in <i>C. bicuspidatus</i>. KEGG enrichment analysis of the expanded gene families showed strong representation of energy-related pathways, particularly pyruvate metabolism. Furthermore, positive selection analysis identified several genes involved in pyruvate metabolism and the citrate cycle (TCA cycle), including specific amino acid substitutions in the pyruvate dehydrogenase complex, a key enzyme in energy conversion. These findings suggest that <i>C. bicuspidatus</i> has undergone coordinated genomic adaptations to enhance energy metabolism, likely to support the high energetic demands of its extended larval development. This study highlights glycolytic adaptation as a key driver of life-history diversification in slipper lobsters.</p>","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"346-356"},"PeriodicalIF":3.6,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147508889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}