Animal Cells and Systems最新文献_第2页

Anti-obesity and immunomodulatory effects of oil and fermented extract dried from Tenebrio molitor larvae on aged obese mice. 褐飞虱幼虫干燥油和发酵提取物对老年肥胖小鼠的抗肥胖和免疫调节作用

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-07-13 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2374547

Seul-Ki Mun, Chang Joo Jang, Semi Jo, Si-Hyoun Park, Hyun Bo Sim, Sonny C Ramos, Hyeongyeong Kim, Yu-Jeong Choi, Dae-Han Park, Kyung-Wuk Park, Beom-Gyun Jeong, Dae Heon Kim, Kyung-Yun Kang, Jong-Jin Kim

{"title":"Anti-obesity and immunomodulatory effects of oil and fermented extract dried from Tenebrio molitor larvae on aged obese mice.","authors":"Seul-Ki Mun, Chang Joo Jang, Semi Jo, Si-Hyoun Park, Hyun Bo Sim, Sonny C Ramos, Hyeongyeong Kim, Yu-Jeong Choi, Dae-Han Park, Kyung-Wuk Park, Beom-Gyun Jeong, Dae Heon Kim, Kyung-Yun Kang, Jong-Jin Kim","doi":"10.1080/19768354.2024.2374547","DOIUrl":"10.1080/19768354.2024.2374547","url":null,"abstract":"Preventing disease and maintaining the health of the elderly are crucial goals for an aging population, with obesity and immune function restoration being of paramount importance. Obesity, particularly visceral obesity characterized by excessive fat accumulation around the abdominal organs, is linked to chronic conditions such as diabetes, hypertension, cardiovascular diseases, and immune dysfunction. Globally, obesity is considered a disease, prompting significant research interest in its treatment. Therefore, it is essential to explore potential therapeutic and preventive strategies to address obesity and the decline in immune function brought about by aging. Tenebrio molitor larvae (TML), commonly known as 'mealworms,' are rich in unsaturated fatty acids, including oleic and linoleic acids, and essential amino acids, such as isoleucine and tyrosine. In this study, we aimed to investigate the effects of the consumption of TML oil and mealworm fermented extract (MWF-1) on obesity and immunological changes in aged obese mice. Our data showed reduced body fat in 23-week-old C57BL/6 mice fed processed TML products for 6 weeks. Additionally, the characteristically high levels of serum triglycerides decreased by treating with TML oil. The immune responsiveness results confirmed an increase in B cells by treating with MWF-1, while cytokine levels (interferon-gamma, tumor necrosis factor-alpha, interleukin-2, and -6) were restored to levels similar to young mice. These results suggest that TML oil and MWF-1 are promising dietary supplements for addressing obesity and restoring immune function.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piperlongumine regulates genes involved in the skin barrier in epidermal keratinocyte HaCaT cells. 胡椒龙葵碱调控表皮角质细胞 HaCaT 细胞中涉及皮肤屏障的基因。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2361144

Kyung-Ha Lee, Deok Gyeong Kang, Dae-Wook Kim, Hwan-Kwon Do, Do-Yeon Kim, Wanil Kim

{"title":"Piperlongumine regulates genes involved in the skin barrier in epidermal keratinocyte HaCaT cells.","authors":"Kyung-Ha Lee, Deok Gyeong Kang, Dae-Wook Kim, Hwan-Kwon Do, Do-Yeon Kim, Wanil Kim","doi":"10.1080/19768354.2024.2361144","DOIUrl":"10.1080/19768354.2024.2361144","url":null,"abstract":"Given that the skin is the largest tissue in the human body, performing external barrier functions with innate and adaptive immunity and undergoing substantial changes during aging, it is under investigation as a major target of various bioactive molecules. In the present study, we examined the biological activity of the senolytic piperlongumine by analyzing alterations in mRNA expression of notable skin genes using transformed aneuploid immortal epidermal keratinocytes, HaCaT cells. We observed that piperlongumine increased the mRNA expression of genes playing critical roles in skin barrier function. In addition, piperlongumine increased expression enzymes involved in the synthesis of ceramide, a major component of intercellular lipids. Furthermore, we measured the protein levels of various cytokines secreted by epidermal keratinocytes and found changes in the release of GRO-αβγ, CCL5, and MCP1. Additionally, we observed that piperlongumine treatment modulated the expression of keratinocyte-specific aging markers and influenced telomerase activity. Based on these findings, piperlongumine could regulate the physiological activity of epidermal keratinocytes to induce beneficial effects in human skin by regulating important skin-related genes.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11207940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a stress granule reporter system for evaluating in vitro colon toxicity. 建立应激颗粒报告系统，用于评估体外结肠毒性。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-06-17 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2364673

Namjoon Cho, Da-Min Jung, Eun-Mi Kim, Kee K Kim

{"title":"Establishment of a stress granule reporter system for evaluating in vitro colon toxicity.","authors":"Namjoon Cho, Da-Min Jung, Eun-Mi Kim, Kee K Kim","doi":"10.1080/19768354.2024.2364673","DOIUrl":"10.1080/19768354.2024.2364673","url":null,"abstract":"Exposure to toxic molecules from food or oral medications induces toxicity in colon cells that cause various human diseases; however, in vitro monitoring systems for colon cell toxicity are not well established. Stress granules are nonmembranous foci that form in cells exposed to cellular stress. When cells sense toxic environments, they acutely and systemically promote stress granule formation, with Ras GTPase-activating protein-binding protein 1 (G3BP1) acting as a core component to protect their mRNA from abnormal degradation. Here, we knocked in green fluorescent protein (GFP)-coding sequences into the C-terminal region of the G3BP1 gene in a human colon cell line through CRISPR-Cas9-mediated homologous recombination and confirmed the formation of stress granules with the G3BP1-GFP protein in these cells under cellular stress exposure. We demonstrated the formation and dissociation of stress granules in G3BP1-GFP expressing colon cells through real-time monitoring using a fluorescence microscope. Furthermore, we validated the toxicity monitoring system in the established colon cell line by observing stress granule formation following exposure to dihydrocapsaicin, bisphenol A, and sorbitol. Taken together, we established a stress granule reporter system in a colon cell line, providing a novel assessment for the real-time monitoring of colon toxicity in response to various chemicals.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte. 在体外生成小鼠和大鼠异种卵巢，用于生产小鼠卵母细胞。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-06-11 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2363601

Si Won Jang, Ye Rim Kim, Jae Ho Han, Hoon Jang, Hyun Woo Choi

{"title":"Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte.","authors":"Si Won Jang, Ye Rim Kim, Jae Ho Han, Hoon Jang, Hyun Woo Choi","doi":"10.1080/19768354.2024.2363601","DOIUrl":"10.1080/19768354.2024.2363601","url":null,"abstract":"The system forming ovarian follicles is developed to investigate in vitro folliculogenesis in a confined environment to obtain functional oocytes. Several studies have reported the successful generation of fully functional oocytes using mouse-induced pluripotent stem cells (iPSCs) and mouse female germline stem cells (fGSCs) as sources of stem cells for in vitro gametogenesis models. In addition, human oogonia have been generated through heterologous co-culture of differentiated human primordial germ cell-like cells (hPGCLCs) with mouse germline somatic cells, although oocyte formation remains challenging. Thus, studies on in vitro ovarian formation in other species are utilized as an introductory approach for in vitro mammalian gametogenesis by understanding the differences in culture systems between species and underlying mechanisms. In this study, we optimized the method of the entire oogenesis process from rat embryonic gonads. We identified well-maturated MII oocytes from rat gonads using our constructed method. Moreover, we generated the first successful in vitro reconstitution of xenogeneic follicles from mouse primordial germ cells (PGCs) and rat somatic cells. We also established an appropriate culture medium and incubation period for xenogeneic follicles. This method will be helpful in studies of xenogeneic follicular development and oocyte generation.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141309462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-autonomous reduction of CYFIP2 changes dendrite length, dendritic protrusion morphology, and inhibitory synapse density in the hippocampal CA1 pyramidal neurons of 17-month-old mice. 细胞自主减少 CYFIP2 会改变 17 个月大小鼠海马 CA1 锥体神经元的树突长度、树突突起形态和抑制性突触密度。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-31 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2360740

Yoonhee Kim, Ruiying Ma, Yinhua Zhang, Hyae Rim Kang, U Suk Kim, Kihoon Han

{"title":"Cell-autonomous reduction of CYFIP2 changes dendrite length, dendritic protrusion morphology, and inhibitory synapse density in the hippocampal CA1 pyramidal neurons of 17-month-old mice.","authors":"Yoonhee Kim, Ruiying Ma, Yinhua Zhang, Hyae Rim Kang, U Suk Kim, Kihoon Han","doi":"10.1080/19768354.2024.2360740","DOIUrl":"10.1080/19768354.2024.2360740","url":null,"abstract":"The cytoplasmic FMR1-interacting protein 2 (CYFIP2) have diverse molecular functions in neurons, including the regulation of actin polymerization, mRNA translation, and mitochondrial morphology and function. Mutations in the CYFIP2 gene are associated with early-onset epilepsy and neurodevelopmental disorders, while decreases in its protein levels are linked to Alzheimer's disease (AD). Notably, previous research has revealed AD-like phenotypes, such as dendritic spine loss, in the hippocampal CA1 pyramidal neurons of 12-month-old Cyfip2 heterozygous mice but not of age-matched CA1 pyramidal neuron-specific Cyfip2 conditional knock-out (cKO) mice. This study aims to investigate whether dendritic spine loss in Cyfip2 cKO mice is merely delayed compared to Cyfip2 heterozygous mice, and to explore further neuronal phenotypes regulated by CYFIP2 in aged mice. We characterized dendrite and dendritic protrusion morphologies, along with excitatory/inhibitory synapse densities in CA1 pyramidal neurons of 17-month-old Cyfip2 cKO mice. Overall dendritic branching was normal, with a reduction in the length of basal, not apical, dendrites in CA1 pyramidal neurons of Cyfip2 cKO mice. Furthermore, while dendritic protrusion density remained normal, alterations were observed in the length of mushroom spines and the head volume of stubby spines in basal, not apical, dendrites of Cyfip2 cKO mice. Although excitatory synapse density remained unchanged, inhibitory synapse density increased in apical, not basal, dendrites of Cyfip2 cKO mice. Consequently, a cell-autonomous reduction of CYFIP2 appears insufficient to induce dendritic spine loss in CA1 pyramidal neurons of aged mice. However, CYFIP2 is required to maintain normal dendritic length, dendritic protrusion morphology, and inhibitory synapse density.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11146249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Producing highly effective extracellular vesicles using IBAR and talin F3 domain fusion. 利用 IBAR 和 talin F3 结构域融合生产高效细胞外囊泡。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-18 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2353159

Joonha Lee, MinHyeong Lee, Jiyoon Kim, Eun-Gyung Cho, Chungho Kim

{"title":"Producing highly effective extracellular vesicles using IBAR and talin F3 domain fusion.","authors":"Joonha Lee, MinHyeong Lee, Jiyoon Kim, Eun-Gyung Cho, Chungho Kim","doi":"10.1080/19768354.2024.2353159","DOIUrl":"10.1080/19768354.2024.2353159","url":null,"abstract":"Extracellular vesicles (EVs), transporting diverse cellular components, play a crucial role in intercellular communication in numerous physiological and pathological processes. EVs have also been recognized as a drug delivery platform for therapeutic purposes and cell-free regenerative medicine. While various approaches have focused on increasing EV production for efficient use therapeutic use of EVs, enhancing the quality of EVs, such as ensuring efficient uptake by their target cells, has not been widely explored. In this study, we linked a negative membrane curvature-forming inverse BAR (IBAR) domain with an integrin β tail-binding talin F3 domain to create the IBAR-F3 fusion protein. We observed that IBAR-F3 can trigger filopodia-like membrane protrusions and attract integrins to those protrusion-rich regions, when expressed in Chinese hamster ovary cells expressing integrin αIIbβ3. Surprisingly, the expression of IBAR-F3 also induced a robust production of EVs, which were then efficiently taken up by nearby cells in an integrin-dependent manner. Moreover, IBAR triggered integrin activation, presumably by inducing negative membrane curvature that likely disrupts the interaction between the integrin α and β transmembrane domain. Therefore, we suggest that IBAR-F3 should be utilized to promote both EV production and efficient uptake mediated by integrins. Furthermore, the negative curvature-inducing integrin activation suggests that integrins on EVs can be activated by the nanoscale change in the curvature of the EV without the need for conventional machinery to activate integrin inside the EVs.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11104707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HSPA5 and FGFR1 genes in the mesenchymal subtype of glioblastoma can improve a treatment efficacy. 间质亚型胶质母细胞瘤中的 HSPA5 和 FGFR1 基因可提高治疗效果。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-18 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2347538

Ju Young Lee, Jongkeun Park, Dongwan Hong

{"title":"HSPA5 and FGFR1 genes in the mesenchymal subtype of glioblastoma can improve a treatment efficacy.","authors":"Ju Young Lee, Jongkeun Park, Dongwan Hong","doi":"10.1080/19768354.2024.2347538","DOIUrl":"10.1080/19768354.2024.2347538","url":null,"abstract":"Tyrosine kinase inhibitors (TKIs) have emerged as a potential treatment strategy for glioblastoma multiforme (GBM). However, their efficacy is limited by various drug resistance mechanisms. To devise more effective treatments for GBM, genetic characteristics must be considered in addition to pre-existing treatments. We performed an integrative analysis with heterogeneous GBM datasets of genomic, transcriptomic, and proteomic data from DepMap, TCGA and CPTAC. We found that poor prognosis was induced by co-upregulation of heat shock protein family A member 5 (HSPA5) and fibroblast growth factor receptor 1 (FGFR1). Co-up regulation of these two genes could regulate the PI3K/AKT pathway. GBM cell lines with co-upregulation of these two genes showed higher drug sensitivity to PI3K inhibitors. In the mesenchymal subtype, the co-upregulation of FGFR1 and HSPA5 resulted in the most malignant subtype of GBM. Furthermore, we found this newly discovered subtype was correlated with homologous recombination deficiency (HRD) In conclusion, we discovered novel druggable candidates within the group exhibiting co-upregulation of these two genes in GBM, suggest potential strategies for combination therapy.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11104699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the structure, chemical composition, and conserved signaling in leech teeth. 揭示水蛭牙齿的结构、化学成分和保守信号。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-11 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2350736

Yam Prasad Aryal, Sanjiv Neupane, Hee-Jin Kwak, Chang-Hyeon An, Wern-Joo Sohn, Hitoshi Yamamoto, Tae-Yub Kwon, Bong-Ki Min, Jae-Young Kim, Sung-Jin Cho

{"title":"Unraveling the structure, chemical composition, and conserved signaling in leech teeth.","authors":"Yam Prasad Aryal, Sanjiv Neupane, Hee-Jin Kwak, Chang-Hyeon An, Wern-Joo Sohn, Hitoshi Yamamoto, Tae-Yub Kwon, Bong-Ki Min, Jae-Young Kim, Sung-Jin Cho","doi":"10.1080/19768354.2024.2350736","DOIUrl":"10.1080/19768354.2024.2350736","url":null,"abstract":"Unlike vertebrates, the number of toothed taxa in invertebrates is very few, with leeches being the only tooth-bearing organisms in the phylum Annelida. Copious studies have been conducted regarding vertebrate teeth; however, studies regarding the structure and function of invertebrate teeth are limited. In this study, the tooth structure of leeches, specifically Hirudo nipponia and Haemadipsa rjukjuana, was revealed, which showed sharp and pointed teeth along the apex of three jaws. Understanding conserved signaling regulations among analogous organs is crucial for uncovering the underlying mechanisms during organogenesis. Therefore, to shed light on the evolutionary perspective of odontogenesis to some extent, we conducted de novo transcriptome analyses using embryonic mouse tooth germs, Hirudo teeth, and Helobdella proboscises to identify conserved signaling molecules involved in tooth development. The selection criteria were particularly based on the presence of tooth-related genes in mice, Hirudo teeth, and Helobdella proboscis, wherein 4113 genes were commonly expressed in all three specimens. Furthermore, the chemical nature of leech teeth was also examined via TEM-EDS to compare the chemical composition with vertebrate teeth. The examination of tissue-specific genetic information and chemical nature between leeches and mice revealed chemical similarities between leech and mice teeth, as well as conserved signaling molecules involved in tooth formation, including Ptpro, Prickle2, and Wnt16. Based on our findings, we propose that leech teeth express signaling molecules conserved in mice and these conserved tooth-specific signaling for dental hard tissue formation in mice would corresponds to the structural formation of the toothed jaw in leeches.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of RNF113A deficiency on oxidative stress-induced NRF2 pathway. RNF113A 缺乏对氧化应激诱导的 NRF2 通路的影响

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-11 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2349758

Namjoon Cho, Yong-Eun Kim, Yunkyeong Lee, Dong Wook Choi, Chungoo Park, Jung-Hwan Kim, Keun Il Kim, Kee K Kim

{"title":"Effect of RNF113A deficiency on oxidative stress-induced NRF2 pathway.","authors":"Namjoon Cho, Yong-Eun Kim, Yunkyeong Lee, Dong Wook Choi, Chungoo Park, Jung-Hwan Kim, Keun Il Kim, Kee K Kim","doi":"10.1080/19768354.2024.2349758","DOIUrl":"10.1080/19768354.2024.2349758","url":null,"abstract":"The ring finger protein 113A (RNF113A) serves as an E3 ubiquitin ligase and a subunit of the spliceosome. Mutations in the RNF113A gene are associated with X-linked trichothiodystrophy (TTD). However, the cellular roles of RNF113A remain largely unknown. In this study, we performed transcriptome profiling of RNF113A knockout (KO) HeLa cells using RNA sequencing and revealed the upregulation of NRF2 pathway-associated genes. Further analysis confirmed that the KO of RNF113A promotes nuclear localization of the NRF2 protein and elevates the mRNA levels of NRF2 target genes. RNF113A KO cells showed high levels of intracellular reactive oxygen species (ROS) and decreased resistance to cell death following H2O2 treatment. Additionally, RNF113A KO cells more sensitively formed stress granules (SGs) under arsenite-induced oxidative stress. Moreover, RNF113A KO cells exhibited a decrease in glutathione levels, which could be attributed to a reduction in GLUT1 expression levels, leading to decreased glucose uptake reactions and lower intracellular glucose levels. These alterations potentially caused a reduction in ROS scavenging activity. Taken together, our findings suggest that the loss of RNF113A promotes oxidative stress-mediated activation of the NRF2 pathway, providing novel insights into RNF113A-associated human diseases.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells. 阻断 SLC7A11 可减轻食管鳞状细胞癌细胞的增殖。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-11 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2346981

Wen-Ting Li, Xin Jin, Sheng-Jie Song, Chong Wang, Chuang Fu, Wen Jiang, Jie Bai, Zhi-Zhou Shi

{"title":"Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells.","authors":"Wen-Ting Li, Xin Jin, Sheng-Jie Song, Chong Wang, Chuang Fu, Wen Jiang, Jie Bai, Zhi-Zhou Shi","doi":"10.1080/19768354.2024.2346981","DOIUrl":"10.1080/19768354.2024.2346981","url":null,"abstract":"The role of ferroptosis-associated gene SLC7A11 in esophageal cancer progression is largely unknown, therefore, the effects of blocking SLC7A11 on esophageal squamous cell carcinoma (ESCC) cells are evaluated. Results showed that SLC7A11 was overexpressed in ESCC tissues both in mRNA and protein levels. Blocking SLC7A11 using Erastin suppressed the proliferation and colony formation of ESCC cells, decreased cellular ATP levels, and improved ROS production. Sixty-three SLC7A11-binding proteins were identified using the IP-MS method, and these proteins were enriched in four signaling pathways, including spliceosome, ribosome, huntington disease, and diabetic cardiomyopathy. The deubiquitinase inhibitors PR-619, GRL0617, and P 22077 could reduce at least 40% protein expression level of SLC7A11 in ESCC cells, and PR-619 and GRL0617 exhibited suppressive effects on the cell viability and colony formation ability of KYSE30 cells, respectively. Erastin downregulated GPX4 and DHODH and also reduced the levels of β-catenin, p-STAT3, and IL-6 in ESCC cells. In conclusion, SLC7A11 was overexpressed in ESCC, and blocking SLC7A11 using Erastin mitigated malignant phenotypes of ESCC cells and downregulated key ferroptosis-associated molecules GPX4 and DHODH. The therapeutic potential of targeting SLC7A11 should be further evaluated in the future.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0