Animal Cells and Systems最新文献_第2页

Lamin A/C facilitates DNA damage response by modulating ATM signaling and homologous recombination pathways. Lamin A/C 通过调节 ATM 信号和同源重组途径促进 DNA 损伤反应。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-08-20 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2393820

Seong-Jung Kim, Su Hyung Park, Kyungjae Myung, Kyoo-Young Lee

{"title":"Lamin A/C facilitates DNA damage response by modulating ATM signaling and homologous recombination pathways.","authors":"Seong-Jung Kim, Su Hyung Park, Kyungjae Myung, Kyoo-Young Lee","doi":"10.1080/19768354.2024.2393820","DOIUrl":"10.1080/19768354.2024.2393820","url":null,"abstract":"Lamin A/C, a core component of the nuclear lamina, forms a mesh-like structure beneath the inner nuclear membrane. While its structural role is well-studied, its involvement in DNA metabolism remains unclear. We conducted sequential protein fractionation to determine the subcellular localization of early DNA damage response (DDR) proteins. Our findings indicate that most DDR proteins, including ATM and the MRE11-RAD50-NBS1 (MRN) complex, are present in the nuclease - and high salt-resistant pellet fraction. Notably, ATM and MRN remain stably associated with these structures throughout the cell cycle, independent of ionizing radiation (IR)-induced DNA damage. Although Lamin A/C interacts with ATM and MRN, its depletion does not disrupt their association with nuclease-resistant structures. However, it impairs the IR-enhanced association of ATM with the nuclear matrix and ATM-mediated DDR signaling, as well as the interaction between ATM and MRN. This disruption impedes the recruitment of MRE11 to damaged DNA and the association of damaged DNA with the nuclear matrix. Additionally, Lamin A/C depletion results in reduced protein levels of CtIP and RAD51, which is mediated by transcriptional regulation. This, in turn, impairs the efficiency of homologous recombination (HR). Our findings indicate that Lamin A/C plays a pivotal role in DNA damage repair (DDR) by orchestrating ATM-mediated signaling, maintaining HR protein levels, and ensuring efficient DNA repair processes.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Licochalcone A attenuates NMDA-induced neurotoxicity. 甘草查尔酮 A 可减轻 NMDA 诱导的神经毒性。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-08-12 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2389823

Jae Soo Kim, Mi-Hye Kim, Myeung Ju Kim, Hee Jung Kim

{"title":"Licochalcone A attenuates NMDA-induced neurotoxicity.","authors":"Jae Soo Kim, Mi-Hye Kim, Myeung Ju Kim, Hee Jung Kim","doi":"10.1080/19768354.2024.2389823","DOIUrl":"10.1080/19768354.2024.2389823","url":null,"abstract":"This study investigates the effect of Licochalcone A (Lico-A), a flavonoid from licorice roots known for its anti-inflammatory, anti-cancer, and antioxidant properties, on NMDA-induced neurotoxicity in primary cultured rat hippocampal neurons. The study measured cell survival following NMDA and Lico-A exposure, revealing that Lico-A at a 2.5 μg/ml significantly improved cell viability, countering the detrimental effects of NMDA. The study also analyzed synaptic changes by examining both postsynaptic density 95 (PSD95) and synaptophysin-targeted imaging, showing that Lico-A treatment resulted in a significant increase in synaptic puncta, contrasting with the reduction observed under NMDA exposure. Furthermore, levels of phosphorylated mixed lineage kinase domain-like pseudokinase (P-MLKL) and phosphorylated receptor-interacting serine/threonine-protein kinase 3 (P-RIP3), key necroptosis regulators, were measured using Western blotting. The results showed an increase in P-MLKL and P-RIP3 in neurons exposed to NMDA, which was reduced following Lico-A treatment. The response of astrocyte and microglia was also evaluated by immunostaining for glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor molecule 1 (IBA-1) and tumor necrosis factor alpha (TNF-α). These markers exhibited heightened expression in the NMDA group, which was substantially reduced by Lico-A treatment. These findings suggest that Lico-A has neuroprotective effects against NMDA-induced neurotoxicity, potentially contributing to synaptic preservation, inhibition of neuronal necroptosis, and modulation of glial activation. Therefore, Lico-A shows promise as a neuroprotective agent for conditions associated with NMDA-related neurotoxicity.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Camellia sinensis L. alleviates OVA-induced allergic asthma through NF-κB and MMP-9 pathways. 山茶通过NF-κB和MMP-9途径缓解OVA诱导的过敏性哮喘

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-08-01 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2383254

So-Won Pak, Ik Soo Lee, Woong-Il Kim, Se-Jin Lee, Jong-Choon Kim, In-Sik Shin, Taesoo Kim

{"title":"Camellia sinensis L. alleviates OVA-induced allergic asthma through NF-κB and MMP-9 pathways.","authors":"So-Won Pak, Ik Soo Lee, Woong-Il Kim, Se-Jin Lee, Jong-Choon Kim, In-Sik Shin, Taesoo Kim","doi":"10.1080/19768354.2024.2383254","DOIUrl":"10.1080/19768354.2024.2383254","url":null,"abstract":"Allergic asthma, a type of chronic airway inflammation, is a global health concern because of its increasing incidence and recurrence rates. Camellia sinensis L. yields a variety type of teas, which are also used as medicinal plants in East Asia and are known to have antioxidant, anti-inflammatory, and immune-potentiating properties. Here, we examined the constituents of C. sinensis L. extract (CSE) and evaluated the protective effects of CSE on allergic asthma by elucidating the underlying mechanism. To induce allergic asthma, we injected the sensitization solution (mixture of ovalbumin (OVA) and aluminum hydroxide) into mice intraperitoneally on days 0 and 14. Then, the mice were exposed to 1% OVA by a nebulizer on days 21 to 23, while intragastric administration of CSE (30 and 100 mg/kg) was performed each day on days 18 to 23. We detected five compounds in CSE, including (-)-epigallocatechin, caffeine, (-)-epicatechin, (-)-epigallocatechin gallate, and (-)-epicatechin gallate. Treatment with CSE remarkably decreased the airway hyperresponsiveness, OVA-specific immunoglobulin E level, and inflammatory cell and cytokine levels of mice, with a decrease in inflammatory cell infiltration and mucus production in lung tissue. Treatment with CSE also decreased the phosphorylation of nuclear factor-κB (NF-κB) and the expression of matrix-metalloproteinase (MMP)-9 in asthmatic mice. Our results demonstrated that CSE reduced allergic airway inflammation caused by OVA through inhibition of phosphorylated NF-κB and MMP-9 expression.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ESCRT-III: a versatile membrane remodeling machinery and its implications in cellular processes and diseases. ESCRT-III：多功能膜重塑机制及其在细胞过程和疾病中的意义。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-07-25 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2380294

Jisoo Park, Jongyoon Kim, Hyungsun Park, Taewan Kim, Seongju Lee

{"title":"ESCRT-III: a versatile membrane remodeling machinery and its implications in cellular processes and diseases.","authors":"Jisoo Park, Jongyoon Kim, Hyungsun Park, Taewan Kim, Seongju Lee","doi":"10.1080/19768354.2024.2380294","DOIUrl":"10.1080/19768354.2024.2380294","url":null,"abstract":"The endosomal sorting complexes required for transport (ESCRT) machinery is an evolutionarily conserved cytosolic protein complex that plays a crucial role in membrane remodeling and scission events across eukaryotes. Initially discovered for its function in multivesicular body (MVB) formation, the ESCRT complex has since been implicated in a wide range of membrane-associated processes, including endocytosis, exocytosis, cytokinesis, and autophagy. Recent advances have elucidated the ESCRT assembly pathway and highlighted the distinct functions of the various ESCRT complexes and their associated partners. Among the ESCRT complexes, ESCRT-III stands out as a critical player in membrane remodeling, with its subunits assembled into higher-order multimers capable of bending and severing membranes. This review focuses on the ESCRT-III complex, exploring its diverse functions in cellular processes beyond MVB biogenesis. We delve into the molecular mechanisms underlying ESCRT-III-mediated membrane remodeling and highlight its emerging roles in processes such as viral budding, autophagosome closure, and cytokinetic abscission. We also discuss the implications of ESCRT-III dysregulation in neurodegenerative diseases. The versatile membrane remodeling capabilities of ESCRT-III across diverse cellular processes underscore its importance in maintaining proper cellular function. Furthermore, we highlight the promising potential of ESCRT-III as a therapeutic target for neurodegenerative diseases, offering insights into the treatments of the diseases and the technical applications in related research fields.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11275535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reassessing the genetic lineage tracing of lingual Lgr5⁺ and Lgr6⁺ cells in vivo. 重新评估舌Lgr5+和Lgr6+细胞在体内的遗传系谱。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-07-21 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2381578

Hyun Ji Kim, Dong Woo Seo, Jaewon Shim, Jun-Seok Lee, Sang-Hyun Choi, Dong-Hoon Kim, Seok Jun Moon, Han-Sung Jung, Yong Taek Jeong

{"title":"Reassessing the genetic lineage tracing of lingual Lgr5+ and Lgr6+ cells in vivo.","authors":"Hyun Ji Kim, Dong Woo Seo, Jaewon Shim, Jun-Seok Lee, Sang-Hyun Choi, Dong-Hoon Kim, Seok Jun Moon, Han-Sung Jung, Yong Taek Jeong","doi":"10.1080/19768354.2024.2381578","DOIUrl":"10.1080/19768354.2024.2381578","url":null,"abstract":"Taste buds, the neuroepithelial organs responsible for the detection of gustatory stimuli in the oral cavity, arise from stem/progenitor cells among nearby basal keratinocytes. Using genetic lineage tracing, Lgr5 and Lgr6 were suggested as the specific markers for the stem/progenitor cells of taste buds, but recent evidence implied that taste buds may arise even in the absence of these markers. Thus, we wanted to verify the genetic lineage tracing of lingual Lgr5- and Lgr6-expressing cells. Unexpectedly, we found that antibody staining revealed more diverse Lgr5-expressing cells inside and outside the taste buds of circumvallate papillae than was previously suggested. We also found that, while tamoxifen-induced genetic recombination occurred only in cells expressing the Lgr5 reporter GFP, we did not see any increase in the number of recombined daughter cells induced by consecutive injections of tamoxifen. Similarly, we found that cells expressing Lgr6, another stem/progenitor cell marker candidate and an analog of Lgr5, also do not generate recombined clones. In contrast, Lgr5-expressing cells in fungiform papillae can transform into Lgr5-negative progeny. Together, our data indicate that lingual Lgr5- and Lgr6-expressing cells exhibit diversity in their capacity to transform into Lgr5- and Lgr6-negative cells, depending on their location. Our results complement previous findings that did not distinguish this diversity.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11262215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity and immunomodulatory effects of oil and fermented extract dried from Tenebrio molitor larvae on aged obese mice. 褐飞虱幼虫干燥油和发酵提取物对老年肥胖小鼠的抗肥胖和免疫调节作用

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-07-13 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2374547

Seul-Ki Mun, Chang Joo Jang, Semi Jo, Si-Hyoun Park, Hyun Bo Sim, Sonny C Ramos, Hyeongyeong Kim, Yu-Jeong Choi, Dae-Han Park, Kyung-Wuk Park, Beom-Gyun Jeong, Dae Heon Kim, Kyung-Yun Kang, Jong-Jin Kim

{"title":"Anti-obesity and immunomodulatory effects of oil and fermented extract dried from Tenebrio molitor larvae on aged obese mice.","authors":"Seul-Ki Mun, Chang Joo Jang, Semi Jo, Si-Hyoun Park, Hyun Bo Sim, Sonny C Ramos, Hyeongyeong Kim, Yu-Jeong Choi, Dae-Han Park, Kyung-Wuk Park, Beom-Gyun Jeong, Dae Heon Kim, Kyung-Yun Kang, Jong-Jin Kim","doi":"10.1080/19768354.2024.2374547","DOIUrl":"10.1080/19768354.2024.2374547","url":null,"abstract":"Preventing disease and maintaining the health of the elderly are crucial goals for an aging population, with obesity and immune function restoration being of paramount importance. Obesity, particularly visceral obesity characterized by excessive fat accumulation around the abdominal organs, is linked to chronic conditions such as diabetes, hypertension, cardiovascular diseases, and immune dysfunction. Globally, obesity is considered a disease, prompting significant research interest in its treatment. Therefore, it is essential to explore potential therapeutic and preventive strategies to address obesity and the decline in immune function brought about by aging. Tenebrio molitor larvae (TML), commonly known as 'mealworms,' are rich in unsaturated fatty acids, including oleic and linoleic acids, and essential amino acids, such as isoleucine and tyrosine. In this study, we aimed to investigate the effects of the consumption of TML oil and mealworm fermented extract (MWF-1) on obesity and immunological changes in aged obese mice. Our data showed reduced body fat in 23-week-old C57BL/6 mice fed processed TML products for 6 weeks. Additionally, the characteristically high levels of serum triglycerides decreased by treating with TML oil. The immune responsiveness results confirmed an increase in B cells by treating with MWF-1, while cytokine levels (interferon-gamma, tumor necrosis factor-alpha, interleukin-2, and -6) were restored to levels similar to young mice. These results suggest that TML oil and MWF-1 are promising dietary supplements for addressing obesity and restoring immune function.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piperlongumine regulates genes involved in the skin barrier in epidermal keratinocyte HaCaT cells. 胡椒龙葵碱调控表皮角质细胞 HaCaT 细胞中涉及皮肤屏障的基因。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2361144

Kyung-Ha Lee, Deok Gyeong Kang, Dae-Wook Kim, Hwan-Kwon Do, Do-Yeon Kim, Wanil Kim

{"title":"Piperlongumine regulates genes involved in the skin barrier in epidermal keratinocyte HaCaT cells.","authors":"Kyung-Ha Lee, Deok Gyeong Kang, Dae-Wook Kim, Hwan-Kwon Do, Do-Yeon Kim, Wanil Kim","doi":"10.1080/19768354.2024.2361144","DOIUrl":"10.1080/19768354.2024.2361144","url":null,"abstract":"Given that the skin is the largest tissue in the human body, performing external barrier functions with innate and adaptive immunity and undergoing substantial changes during aging, it is under investigation as a major target of various bioactive molecules. In the present study, we examined the biological activity of the senolytic piperlongumine by analyzing alterations in mRNA expression of notable skin genes using transformed aneuploid immortal epidermal keratinocytes, HaCaT cells. We observed that piperlongumine increased the mRNA expression of genes playing critical roles in skin barrier function. In addition, piperlongumine increased expression enzymes involved in the synthesis of ceramide, a major component of intercellular lipids. Furthermore, we measured the protein levels of various cytokines secreted by epidermal keratinocytes and found changes in the release of GRO-αβγ, CCL5, and MCP1. Additionally, we observed that piperlongumine treatment modulated the expression of keratinocyte-specific aging markers and influenced telomerase activity. Based on these findings, piperlongumine could regulate the physiological activity of epidermal keratinocytes to induce beneficial effects in human skin by regulating important skin-related genes.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11207940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a stress granule reporter system for evaluating in vitro colon toxicity. 建立应激颗粒报告系统，用于评估体外结肠毒性。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-06-17 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2364673

Namjoon Cho, Da-Min Jung, Eun-Mi Kim, Kee K Kim

{"title":"Establishment of a stress granule reporter system for evaluating in vitro colon toxicity.","authors":"Namjoon Cho, Da-Min Jung, Eun-Mi Kim, Kee K Kim","doi":"10.1080/19768354.2024.2364673","DOIUrl":"10.1080/19768354.2024.2364673","url":null,"abstract":"Exposure to toxic molecules from food or oral medications induces toxicity in colon cells that cause various human diseases; however, in vitro monitoring systems for colon cell toxicity are not well established. Stress granules are nonmembranous foci that form in cells exposed to cellular stress. When cells sense toxic environments, they acutely and systemically promote stress granule formation, with Ras GTPase-activating protein-binding protein 1 (G3BP1) acting as a core component to protect their mRNA from abnormal degradation. Here, we knocked in green fluorescent protein (GFP)-coding sequences into the C-terminal region of the G3BP1 gene in a human colon cell line through CRISPR-Cas9-mediated homologous recombination and confirmed the formation of stress granules with the G3BP1-GFP protein in these cells under cellular stress exposure. We demonstrated the formation and dissociation of stress granules in G3BP1-GFP expressing colon cells through real-time monitoring using a fluorescence microscope. Furthermore, we validated the toxicity monitoring system in the established colon cell line by observing stress granule formation following exposure to dihydrocapsaicin, bisphenol A, and sorbitol. Taken together, we established a stress granule reporter system in a colon cell line, providing a novel assessment for the real-time monitoring of colon toxicity in response to various chemicals.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11185092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141417351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte. 在体外生成小鼠和大鼠异种卵巢，用于生产小鼠卵母细胞。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-06-11 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2363601

Si Won Jang, Ye Rim Kim, Jae Ho Han, Hoon Jang, Hyun Woo Choi

{"title":"Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte.","authors":"Si Won Jang, Ye Rim Kim, Jae Ho Han, Hoon Jang, Hyun Woo Choi","doi":"10.1080/19768354.2024.2363601","DOIUrl":"10.1080/19768354.2024.2363601","url":null,"abstract":"The system forming ovarian follicles is developed to investigate in vitro folliculogenesis in a confined environment to obtain functional oocytes. Several studies have reported the successful generation of fully functional oocytes using mouse-induced pluripotent stem cells (iPSCs) and mouse female germline stem cells (fGSCs) as sources of stem cells for in vitro gametogenesis models. In addition, human oogonia have been generated through heterologous co-culture of differentiated human primordial germ cell-like cells (hPGCLCs) with mouse germline somatic cells, although oocyte formation remains challenging. Thus, studies on in vitro ovarian formation in other species are utilized as an introductory approach for in vitro mammalian gametogenesis by understanding the differences in culture systems between species and underlying mechanisms. In this study, we optimized the method of the entire oogenesis process from rat embryonic gonads. We identified well-maturated MII oocytes from rat gonads using our constructed method. Moreover, we generated the first successful in vitro reconstitution of xenogeneic follicles from mouse primordial germ cells (PGCs) and rat somatic cells. We also established an appropriate culture medium and incubation period for xenogeneic follicles. This method will be helpful in studies of xenogeneic follicular development and oocyte generation.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141309462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-autonomous reduction of CYFIP2 changes dendrite length, dendritic protrusion morphology, and inhibitory synapse density in the hippocampal CA1 pyramidal neurons of 17-month-old mice. 细胞自主减少 CYFIP2 会改变 17 个月大小鼠海马 CA1 锥体神经元的树突长度、树突突起形态和抑制性突触密度。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2024-05-31 eCollection Date: 2024-01-01 DOI: 10.1080/19768354.2024.2360740

Yoonhee Kim, Ruiying Ma, Yinhua Zhang, Hyae Rim Kang, U Suk Kim, Kihoon Han

{"title":"Cell-autonomous reduction of CYFIP2 changes dendrite length, dendritic protrusion morphology, and inhibitory synapse density in the hippocampal CA1 pyramidal neurons of 17-month-old mice.","authors":"Yoonhee Kim, Ruiying Ma, Yinhua Zhang, Hyae Rim Kang, U Suk Kim, Kihoon Han","doi":"10.1080/19768354.2024.2360740","DOIUrl":"10.1080/19768354.2024.2360740","url":null,"abstract":"The cytoplasmic FMR1-interacting protein 2 (CYFIP2) have diverse molecular functions in neurons, including the regulation of actin polymerization, mRNA translation, and mitochondrial morphology and function. Mutations in the CYFIP2 gene are associated with early-onset epilepsy and neurodevelopmental disorders, while decreases in its protein levels are linked to Alzheimer's disease (AD). Notably, previous research has revealed AD-like phenotypes, such as dendritic spine loss, in the hippocampal CA1 pyramidal neurons of 12-month-old Cyfip2 heterozygous mice but not of age-matched CA1 pyramidal neuron-specific Cyfip2 conditional knock-out (cKO) mice. This study aims to investigate whether dendritic spine loss in Cyfip2 cKO mice is merely delayed compared to Cyfip2 heterozygous mice, and to explore further neuronal phenotypes regulated by CYFIP2 in aged mice. We characterized dendrite and dendritic protrusion morphologies, along with excitatory/inhibitory synapse densities in CA1 pyramidal neurons of 17-month-old Cyfip2 cKO mice. Overall dendritic branching was normal, with a reduction in the length of basal, not apical, dendrites in CA1 pyramidal neurons of Cyfip2 cKO mice. Furthermore, while dendritic protrusion density remained normal, alterations were observed in the length of mushroom spines and the head volume of stubby spines in basal, not apical, dendrites of Cyfip2 cKO mice. Although excitatory synapse density remained unchanged, inhibitory synapse density increased in apical, not basal, dendrites of Cyfip2 cKO mice. Consequently, a cell-autonomous reduction of CYFIP2 appears insufficient to induce dendritic spine loss in CA1 pyramidal neurons of aged mice. However, CYFIP2 is required to maintain normal dendritic length, dendritic protrusion morphology, and inhibitory synapse density.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11146249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0