Animal Cells and Systems最新文献_第3页

Comparative phylomitogenomic analyses provide insights into adaptation and carcinization in Anomura. 比较动物组织基因组学分析提供了对畸形的适应和癌变的见解。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2026-01-12 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2025.2607863

Hee-Seung Hwang, Jibom Jung

{"title":"Comparative phylomitogenomic analyses provide insights into adaptation and carcinization in Anomura.","authors":"Hee-Seung Hwang, Jibom Jung","doi":"10.1080/19768354.2025.2607863","DOIUrl":"10.1080/19768354.2025.2607863","url":null,"abstract":"Anomura is a morphologically and ecologically diverse infraorder of decapod crustaceans, yet its evolutionary and phylogenetic patterns remain underexplored using mitochondrial genome-based approaches, particularly regarding adaptive evolution across diverse environments. Here, we present a comprehensive phylogenomic analysis of 42 anomuran mitochondrial genomes, including three newly sequenced species: two intertidal Hapalogastrinae (Hapalogaster dentata and Oedignathus inermis) and one deep-sea pagurid (Pagurus rathbuni). The arrangement of protein-coding genes was identical to that reported in previously studied Lithodidae and Paguridae species; however, several tRNA genes exhibited translocations. Moreover, more than half of the 22 tRNA genes were predicted to adopt atypical cloverleaf form, and all protein-coding genes were under purifying selection. In addition, analysis of the mitochondrial control region revealed a conserved repeat structure (∼47 bp motif repeated ∼3.6 times), from which depth-associated Gibbs free energy patterns were broadly inferred. These patterns suggest potential links between control-region stability, depth-dependent adaptation, and the evolutionary process of carcinization. Time-calibrated analyses suggest that H. dentata and O. inermis diverged from other lithodids approximately 37-50 million years ago, while P. rathbuni diverged around 32 million years ago. These divergence events coincide with the Eocene-Oligocene transition, a period characterized by global cooling, sea-level decline, and shifts in ocean circulation. This temporal correspondence suggests that such environmental changes may have been associated with the diversification and adaptive evolution of Anomura. Overall, this study advances our understanding of anomuran phylogeny and highlights the complex interplay among adaptation to the environment, carcinization, and mitochondrial genome evolution.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"13-33"},"PeriodicalIF":3.2,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12798672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sumoylation of cyclin and its therapeutic potential for cancer. 细胞周期蛋白的sumo化及其对癌症的治疗潜力。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-12-06 eCollection Date: 2026-01-01 DOI: 10.1080/19768354.2025.2598975

Hong-Yeoul Ryu, Mark Hochstrasser

{"title":"Sumoylation of cyclin and its therapeutic potential for cancer.","authors":"Hong-Yeoul Ryu, Mark Hochstrasser","doi":"10.1080/19768354.2025.2598975","DOIUrl":"10.1080/19768354.2025.2598975","url":null,"abstract":"The precision of the cell cycle is essential for organismal development, tissue homeostasis, and the prevention of malignancies. Cyclins and cyclin-dependent kinases (CDKs) play pivotal roles in regulating cell cycle progression. Recent studies have underscored the importance of post-translational modifications, particularly sumoylation, in modulating the functions of cyclins. Sumoylation profoundly influences the stability, localization, and activity of cyclins D and E, which are crucial for the G1/S transition and DNA replication. Dysregulation of these processes is a hallmark of various cancers, where aberrant sumoylation enhances the oncogenic potential of cyclins. This review examines how sumoylation governs cyclin dynamics, maintains cell division fidelity, and contributes to cancer progression. Moreover, advances in targeting the SUMO pathway offer new therapeutic opportunities for treating cyclin-related malignancies, positioning sumoylation-based strategies as promising tools in precision medicine. Gaining a deeper understanding of how sumoylation regulates cyclins may ultimately transform therapeutic approaches for cyclin-dependent diseases.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"30 1","pages":"71-82"},"PeriodicalIF":3.2,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12683770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145712992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in regulation of aging by non-coding RNAs in Caenorhabditis elegans. 秀丽隐杆线虫非编码rna调控衰老的研究进展。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-11-27 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2593037

Seungjae Hwang, Woojin Hong, Seung-Jae V Lee

引用次数: 0

Epinephrine as a potential driver of oral lichen planus pathogenesis. 肾上腺素作为口腔扁平苔藓发病的潜在驱动因素。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-11-17 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2588914

Yu Gyung Kim, Kun-Hwa Kang, Hyo-Jin Song, Won Jung, Sungil Jang, Jin-Seok Byun, Do-Yeon Kim

{"title":"Epinephrine as a potential driver of oral lichen planus pathogenesis.","authors":"Yu Gyung Kim, Kun-Hwa Kang, Hyo-Jin Song, Won Jung, Sungil Jang, Jin-Seok Byun, Do-Yeon Kim","doi":"10.1080/19768354.2025.2588914","DOIUrl":"10.1080/19768354.2025.2588914","url":null,"abstract":"Oral lichen planus (OLP) is a chronic inflammatory condition characterized by CD8+ T cell-mediated apoptosis of oral epithelial cells. While psychological stress has been implicated in OLP pathogenesis, the underlying mechanisms remain unclear. This study explores the role of epinephrine, a primary stress-related catecholamine, in OLP progression. We found that high concentrations of epinephrine induce cytotoxicity in oral keratinocytes, marked by reduced cell viability and increased DNA damage. High-dose epinephrine also elevates oxidative stress by downregulating antioxidant proteins SOD2 and SESN2. Additionally, it activates the STAT3 signaling pathway through both alpha- and beta-adrenergic receptors. Furthermore, epinephrine increases levels of HMGB1 and extracellular ATP, key damage-associated molecular patterns (DAMPs) that could perpetuate chronic inflammation in OLP. These findings suggest that stress-induced epinephrine may exacerbate OLP by promoting oxidative stress, epithelial damage, and immune activation. Given the increased vascularization in OLP lesions, epinephrine's effects may be amplified in affected tissues. Understanding the link between stress and OLP pathogenesis could provide new therapeutic targets for managing this condition.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"653-664"},"PeriodicalIF":3.2,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12624973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145556112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel TLR2 agonist Amuc_C derived from Akkermansia muciniphila exhibits potent anti-tumor activity in colorectal cancers. 新型TLR2激动剂Amuc_C来源于嗜粘Akkermansia muciniphila，在结直肠癌中显示出强大的抗肿瘤活性。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-10-27 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2578019

Liang Chi, Chiao-Hsu Ke, Hsin-Yi Wu, I-Li Liu, Chih-Hung Huang, Chen-Si Lin

{"title":"Novel TLR2 agonist Amuc_C derived from Akkermansia muciniphila exhibits potent anti-tumor activity in colorectal cancers.","authors":"Liang Chi, Chiao-Hsu Ke, Hsin-Yi Wu, I-Li Liu, Chih-Hung Huang, Chen-Si Lin","doi":"10.1080/19768354.2025.2578019","DOIUrl":"10.1080/19768354.2025.2578019","url":null,"abstract":"Colorectal cancer (CRC) is a challenging disease. Recent studies have gradually emphasized the development of novel immunotherapies rather than traditional treatments. Toll-like receptor (TLR) agonists are critical in innate immune responses to orchestrate anti-tumor efficacies, which are attributed to their aptitude to stimulate antigen-presenting cells (APCs) and thus activate tumor-specific T cells. Although several TLR agonists have been proposed for treating tumors, their therapeutic efficacy remains controversial. Therefore, the current study aimed to develop a novel TLR2 agonist, Amuc_1100 C-terminal (Amuc_C), a purified membrane protein from Akkermansia muciniphila (A. muciniphila), and evaluate its anti-tumor properties. Herein, a murine CRC model, CT26, was employed. Tumor-bearing mice received intertumoral treatment with Amuc_C. The anti-tumor effects were determined by flow cytometry, cytokine enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assays. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was then employed to uncover the potent mechanisms. Amuc_C significantly increased the amounts of tumor-infiltrating lymphocytes and systemic immune cells, especially cytotoxic T cells, M1 macrophages, and type 1 dendritic cells. Furthermore, Amuc_C triggered IL-1β, TNF-α, and IFN-γ productions, significantly decreasing tumor growth, and prolonged overall survival. The immunotherapeutic mechanisms revealed by proteomics data were related to the activation of immune responses, the induction of cell cycle arrest, and the inhibition of cell proliferative signaling pathways. In summary, the current study has demonstrated that administration of Amuc_C improves the APCs and escalates adaptive anti-tumor immunity. With the demand for effective anti-tumor treatments, our results provide a compelling proof-of-concept of a TLR2 agonist for cancer immunotherapy.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"628-642"},"PeriodicalIF":3.2,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12570232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down-regulation of HSPA9 reduces tyrosine hydroxylase-positive neurons in mouse substantia nigra and induces Parkinson's disease-like motor impairments. HSPA9的下调减少了小鼠黑质中酪氨酸羟酶阳性神经元并诱导帕金森病样运动损伤。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-10-14 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2569875

Hyejin Hyung, Soyoung Jang, Si-Yong Kim, Ji-Eun Bae, Ji Yeong Park, Su-Geun Lim, Jiwon Ko, Soyeon Jang, Joon Bum Kim, Hee Young Chae, Song Park, Junkoo Yi, Dong Kyu Choi, Myoung Ok Kim, Hyun-Shik Lee, Dong-Hyung Cho, Zae Young Ryoo

{"title":"Down-regulation of HSPA9 reduces tyrosine hydroxylase-positive neurons in mouse substantia nigra and induces Parkinson's disease-like motor impairments.","authors":"Hyejin Hyung, Soyoung Jang, Si-Yong Kim, Ji-Eun Bae, Ji Yeong Park, Su-Geun Lim, Jiwon Ko, Soyeon Jang, Joon Bum Kim, Hee Young Chae, Song Park, Junkoo Yi, Dong Kyu Choi, Myoung Ok Kim, Hyun-Shik Lee, Dong-Hyung Cho, Zae Young Ryoo","doi":"10.1080/19768354.2025.2569875","DOIUrl":"10.1080/19768354.2025.2569875","url":null,"abstract":"Parkinson's disease (PD) is a progressive neurological disorder characterized by the degeneration of midbrain dopaminergic neurons and disabling motor impairments. Heat shock protein family A member 9 (HSPA9) play a crucial role in neuronal homeostasis by regulating the import of various mitochondrial proteins. HSPA9 is down-regulated in neurodegenerative diseases such as Alzheimer's disease and PD, and its loss leads to excessive mitochondrial fragmentation with oxidative stress, which subsequently causes damage to dopaminergic neurons. Moreover, HSPA9 interacts with multiple PD-associated proteins, including Pink1, DJ-1, and α-synuclein, however precise roles of HSPA9 in PD pathophysiology remain unclear. To further explore the contributions of HSPA9 in PD pathogenesis, we developed an HSPA9 knockout mouse. Haploinsufficiency of Hspa9 (Hspa9 +/-) was associated with the loss of tyrosine hydroxylase-positive neurons in the striatum and substantia nigra. Furthermore, Hspa9 haploinsufficiency induced excessive mitochondrial fission, enhanced apoptotic signaling, and resulted in diminished motor performance during the rotarod test. Administration of the mitochondrial neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in Hspa9 +/- mice further exacerbated the loss of dopaminergic neurons, aggravated motor impairments, and enhanced activation of apoptosis effector caspase-3. These results suggest that down-regulation of HSPA9 may contribute to the development and progression of PD, potentially offering a new therapeutic strategy for PD treatment.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"615-627"},"PeriodicalIF":3.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA-mRNA integrated networks in the neuroendocrine system of bisphenol a-treated mice induce cellular dysfunctions by disrupting transcriptional homeostasis. 双酚a处理小鼠神经内分泌系统中的LncRNA-mRNA整合网络通过破坏转录稳态诱导细胞功能障碍。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-10-11 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2569881

Seung-Mi Oh, Byeonghwi Lim, Yoon-Been Park, Min-Jae Jang, Seok-Won Lim, Chiwoong Lim, Do-Young Kim, Yejee Park, Young-Jun Seo, Jun-Mo Kim

{"title":"LncRNA-mRNA integrated networks in the neuroendocrine system of bisphenol a-treated mice induce cellular dysfunctions by disrupting transcriptional homeostasis.","authors":"Seung-Mi Oh, Byeonghwi Lim, Yoon-Been Park, Min-Jae Jang, Seok-Won Lim, Chiwoong Lim, Do-Young Kim, Yejee Park, Young-Jun Seo, Jun-Mo Kim","doi":"10.1080/19768354.2025.2569881","DOIUrl":"10.1080/19768354.2025.2569881","url":null,"abstract":"Bisphenol A (BPA) is a widely used xenoestrogen that can disrupt neuroendocrine and immune regulation through multiple hormone receptors. This study investigated BPA-induced long non-coding RNA (lncRNA)-mRNA interactions in the cerebral cortex and hypothalamic-pituitary-thyroid (HPT) axis of adult male mice. Transcriptome sequencing and comprehensive lncRNA annotation identified 14,858 novel lncRNA transcripts. Integrated network analysis using weighted gene co-expression network analysis (WGCNA) revealed four distinct tissue-specific modules: neuronal signaling alterations (Tac1, Htr1b, Npy), RNA splicing modifications (Srsf5), PI3K/Akt-mediated cellular dysfunction (Creb5, Cdkn1a), and immune receptor signaling disruptions (Trbv15, Fcrla). These findings suggest that BPA reprograms transcriptional networks in a tissue-specific manner, potentially disrupting hormone-related neurotransmission, metabolic regulation, and immune signaling via lncRNA-mediated mechanisms. Such systems-level reprogramming of the immune-neuroendocrine network (INEN) provides novel mechanistic insights and biomarker candidates for assessing and mitigating the health impacts of environmental endocrine disruptors.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"598-614"},"PeriodicalIF":3.2,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12517422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of BAT thermogenesis via TRPA1-expressing hypothalamic POMC neurons. 表达trpa1的下丘脑POMC神经元对BAT产热的调控。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-09-29 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2559611

Arbi Bahtiar Boedi Iman Halanobis, Ju Hwan Yang, Eun-Hye Byeon, Sang Won Park, Hyun Joon Kim, Dawon Kang, Deok-Ryong Kim, Jinsung Yang, Wanil Kim, Dong-Hee Kim, Dong Kun Lee

{"title":"Regulation of BAT thermogenesis via TRPA1-expressing hypothalamic POMC neurons.","authors":"Arbi Bahtiar Boedi Iman Halanobis, Ju Hwan Yang, Eun-Hye Byeon, Sang Won Park, Hyun Joon Kim, Dawon Kang, Deok-Ryong Kim, Jinsung Yang, Wanil Kim, Dong-Hee Kim, Dong Kun Lee","doi":"10.1080/19768354.2025.2559611","DOIUrl":"10.1080/19768354.2025.2559611","url":null,"abstract":"Pro-opiomelanocortin (POMC) neurons in the hypothalamic arcuate nucleus (ARC) play a pivotal role in regulating brown adipose tissue (BAT) thermogenesis via the sympathetic nervous system. The activation of transient receptor potential ankyrin 1 (TRPA1) has been demonstrated to enhance heat production, particularly in BAT. However, no direct evidence has been reported regarding BAT thermogenesis mediated by TRPA1-regulated ARC POMC neurons. This study aimed to investigate the role of TRPA1-expressing hypothalamic POMC neurons in BAT thermogenesis. To confirm TRPA1 expression in ARC POMC neurons, we employed single-cell reverse transcriptase polymerase chain reaction and immunolabeling techniques. Selective TRPA1 agonists, including capsiate and ASP7663, induced depolarization of ARC POMC neurons, an effect that was inhibited by A967079, a TRPA1-selective antagonist. Furthermore, intracerebroventricular (i.c.v.) administration of ASP7663 increased BAT and core body temperature. The thermogenic effect of ASP7663 in BAT was abolished by co-administration of A967079. Among the BAT thermogenic markers, peroxisome proliferator-activated receptor gamma coactivator 1-alpha and PR domain containing 16 (PRDM16) expressions were considerably upregulated following i.c.v. administration of ASP7663. However, this increase was reversed by A967079, except for PRDM16. These findings indicate that TRPA1-mediated activation of hypothalamic POMC neurons is critical in regulating BAT thermogenesis and promoting energy expenditure.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"584-597"},"PeriodicalIF":3.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential effects of nesfatin-1 on proliferation and migration in normal and cancerous human lung cells via the PI3K/AKT pathway. nesfatin-1通过PI3K/AKT通路对正常和癌变人肺细胞增殖和迁移的差异影响

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-09-15 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2542162

Eunji Im, Jinah Ha, Jeongha Kim, Hyunwon Yang

{"title":"Differential effects of nesfatin-1 on proliferation and migration in normal and cancerous human lung cells via the PI3K/AKT pathway.","authors":"Eunji Im, Jinah Ha, Jeongha Kim, Hyunwon Yang","doi":"10.1080/19768354.2025.2542162","DOIUrl":"10.1080/19768354.2025.2542162","url":null,"abstract":"Nesfatin-1, initially identified as an appetite-regulating hormone, has also been detected in various cancer tissues and implicated in tumorigenesis. However, its role in the proliferation and migration of lung cancer cells remains unclear. This study aims to investigate the effects of nesfatin-1 on the proliferation and migration of human lung cancer cells and elucidate the underlying molecular mechanisms. The expression of nesfatin-1 protein and NUCB2 mRNA was detected in the immortalized normal human bronchial cell line BEAS-2B and the non-small-cell lung cancer cell line H1299. Immunohistochemical staining revealed the localization of nesfatin-1 binding sites in both cell lines. Nesfatin-1 treatment significantly increased the proliferation and migration of BEAS-2B cells but not of H1299 cells. The expression levels of cell proliferation-related genes, such as TGFα, PXN, MTOR, and CCND1, were upregulated in BEAS-2B cells, with no significant changes observed in H1299 cells. In addition, phosphorylation of FAK, PI3 K, and AKT was increased in BEAS-2B cells, whereas only FAK phosphorylation was increased in H1299 cells. To further assess the role of endogenous nesfatin-1, NUCB2 expression was silenced using small interfering RNA. Knockdown of NUCB2 suppressed proliferation and migration of BEAS-2B cells, as well as their expression of TGFα, PXN, MTOR, and CCND1; however, it had no significant effect on H1299 cells. These results suggest that nesfatin-1 promotes proliferation and migration in normal lung epithelial cells but not in lung cancer cells. Further research is needed to elucidate the molecular mechanisms underlying the differential effects of nesfatin-1 on normal and cancerous lung cells.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"570-583"},"PeriodicalIF":3.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study of capacitation-mediated changes in whole mouse sperm proteome. 全鼠精子蛋白质组获能介导变化的比较研究。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-08-28 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2548936

Jae Yeon Hwang

{"title":"A comparative study of capacitation-mediated changes in whole mouse sperm proteome.","authors":"Jae Yeon Hwang","doi":"10.1080/19768354.2025.2548936","DOIUrl":"10.1080/19768354.2025.2548936","url":null,"abstract":"Mammalian spermatozoa acquire fertilizing ability in response to environmental factors enriched in the female reproductive tract, a process called capacitation. During capacitation, sperm undergo physiological changes that are accompanied by functional regulation of sperm proteins. However, the mechanism by which capacitation orchestrates sperm protein functions to modulate physiological characteristics remains unclear. Here, I analyzed capacitation-mediated global proteomic changes in mouse spermatozoa to unravel the underlying molecular association with the biological processes in sperm capacitation. I quantitatively compared 4,587 proteins identified by liquid chromatography-tandem mass spectrometry. Among them, the amounts of 47 and 180 proteins were reduced to over 1.5-fold (p < 0.05) and 1.3-fold (p < 0.1), respectively, and those of 11 and 60 proteins were increased over 1.5-fold (p < 0.05) and 1.3-fold (p < 0.1), respectively, in capacitated mouse sperm. Functional annotation of proteins reduced in capacitated sperm revealed that these proteins could be associated with lipid metabolism, RNA processing, and remodeling of the nuclear envelope structure. This result suggests that reactive oxygen species might be more generated for cholesterol efflux and the nucleus might undergo decondensation to form pronucleus in sperm during capacitation. In addition, functional annotation of proteins of which levels are increased in capacitated sperm represents that they could be involved in sperm structure. This study expands the molecular contribution to modulation of sperm functions and provides new insights into potential biological processes involving regulatory molecular machinery in capacitated sperm.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"556-569"},"PeriodicalIF":3.2,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0