Animal Cells and Systems最新文献_第4页

The RNA revolution in medicine: from gene regulation to clinical therapeutics. 医学中的RNA革命：从基因调控到临床治疗。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-08-25 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2548253

Jiwon Jeong, Sunjoo Jeong

{"title":"The RNA revolution in medicine: from gene regulation to clinical therapeutics.","authors":"Jiwon Jeong, Sunjoo Jeong","doi":"10.1080/19768354.2025.2548253","DOIUrl":"10.1080/19768354.2025.2548253","url":null,"abstract":"Breakthrough discoveries in RNA biology have led to a paradigm shift in our understanding of RNA-from passive intermediates to active regulators of gene expression. Technological innovations and deeper insights into RNA regulation have transformed the field, positioning RNA as a powerful tool for therapeutic development. Recent advances in RNA technologies have revolutionized medicine by enabling the precise targeting of specific mRNAs to modulate aberrant transcripts, correct genetic defects, and reprogram cellular behavior. This review provides an overview of the coordinated regulation of mRNA processing and its application to RNA-based therapeutics, including antisense oligonucleotides (ASOs), splice-switching oligonucleotides (SSOs), small interfering RNAs (siRNAs), and Aptamers. We focus on clinically approved RNA therapeutics, emphasizing their biological mechanisms such as RNA stability and splicing regulation. The expanding repertoire of RNA technologies underscores the translational potential of RNA biology and its growing clinical impact. Future developments are expected to yield highly specific, modular, and programmable RNA medicines capable of treating a wide range of previously intractable diseases.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"523-543"},"PeriodicalIF":3.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12379706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xanthoxyline prevents aging and neuronal damage by activating autophagy and DAF-16 expression in Caenorhabditis elegans. 黄多酚通过激活秀丽隐杆线虫的自噬和DAF-16表达来防止衰老和神经元损伤。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2549756

Hyemin Kim, Saebyeol Lee, Hak Kyun Kim, Sang-Kyu Park

{"title":"Xanthoxyline prevents aging and neuronal damage by activating autophagy and DAF-16 expression in Caenorhabditis elegans.","authors":"Hyemin Kim, Saebyeol Lee, Hak Kyun Kim, Sang-Kyu Park","doi":"10.1080/19768354.2025.2549756","DOIUrl":"10.1080/19768354.2025.2549756","url":null,"abstract":"Xanthoxyline, a plant-derived phytochemical, has anti-bacterial, anti-fungal, and anti-cancer activities. We intended to investigate the effect of xanthoxyline on the response to oxidative stress, aging, and Parkinson's disease. The effects of dietary supplementation with xanthoxyline on stress response and aging were examined in vivo using Caenorhabditis elegans as a model system. Genetic analysis using mutants, RNAi, and quantitative RT-PCR was performed to identify underlying mechanism involved in xanthoxyline-induced longevity. Animal disease models were employed to examine the effect of xanthoxyline on Parkinson's disease. Xanthoxyline increased resistance to the oxidative stress induced by H2O2. The mean lifespan of worms was significantly increased by supplementation with xanthoxyline. The lifespan-extending activity of xanthoxyline was not accompanied by reduced fertility. Xanthoxyline delayed the age-related decline in motility. Interestingly, the expression of two longevity-assuring genes, hsp-16.2, and sod-3, was increased by xanthoxyline supplementation. Genetic analysis suggested that lifespan extension by xanthoxyline was mediated by activation of autophagy and required DAF-16. In a model of Parkinson's disease, degeneration of dopaminergic neurons was prevented by supplementation with xanthoxyline, in a manner dependent on DAF-16. Taken together, we concluded that xanthoxyline exerts an anti-aging activity, possibly by activating the DAF-16-dependent stress response, and reduces the risk of Parkinson's disease, in a manner mediated by DAF-16. Xanthoxyline shows promise for the development of novel nutraceuticals against aging and Parkinson's disease.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"544-555"},"PeriodicalIF":3.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression. RNA结合蛋白HuD通过调节长链酰基辅酶a脱氢酶表达调控胰腺β细胞脂肪酸氧化。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-08-11 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2542168

Jiyoon Seo, Seungyeon Ryu, Wei Zhang, Eun Kyung Lee, Seung Min Jeong

引用次数: 0

Targeting MMR-deficient colorectal cancer with a potent small molecule UNI110. 靶向mmr缺陷结直肠癌的有效小分子UNI110。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2542172

Enkhzul Amarsanaa, Jung-Min Oh, Seon Young Lee, Saikat Maiti, Sung You Hong, Kyungjae Myung

{"title":"Targeting MMR-deficient colorectal cancer with a potent small molecule UNI110.","authors":"Enkhzul Amarsanaa, Jung-Min Oh, Seon Young Lee, Saikat Maiti, Sung You Hong, Kyungjae Myung","doi":"10.1080/19768354.2025.2542172","DOIUrl":"10.1080/19768354.2025.2542172","url":null,"abstract":"Mismatch repair (MMR) deficiency is a hallmark of microsatellite instability (MSI) in hereditary non-polyposis colorectal cancer, Lynch syndrome, contributing to resistance against conventional chemotherapy and posing a significant therapeutic challenge. In this study, we introduce UNI110, a novel small molecule derived from Baicalein, engineered for enhanced selectivity against MMR-deficient cancer cells. UNI110 exhibits a remarkable sevenfold increase in potency over Baicalein, demonstrating significantly lower IC50 values and heightened cytotoxic effects in MMR-deficient cell lines. Mechanistically, UNI110 selectively induces DNA damage in MMR-deficient cancer cells, ultimately resulting in cell death. Furthermore, UNI110 disrupts homologous recombination (HR) repair by inhibiting the MSH2-MSH3 complex, specifically blocking the interaction between MSH2 and EXO1, thereby impairing long-range end resection during double-strand break (DSB) repair. These findings establish UNI110 as a promising lead compound for the targeted treatment of MMR-deficient colorectal cancers, offering a potential breakthrough in overcoming chemotherapy resistance and improving patient outcomes.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"502-511"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asxl1 regulates optic cup development through interaction with Lhx2 and epigenetic modulation of Wnt signaling. Asxl1通过与Lhx2的相互作用和Wnt信号的表观遗传调节来调节视杯的发育。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2542176

Seungtae Moon, Nackhyuong Kim, A-Reum Kim, Kyeong Hwan Moon, Jin Woo Kim, Soo-Jong Um

{"title":"Asxl1 regulates optic cup development through interaction with Lhx2 and epigenetic modulation of Wnt signaling.","authors":"Seungtae Moon, Nackhyuong Kim, A-Reum Kim, Kyeong Hwan Moon, Jin Woo Kim, Soo-Jong Um","doi":"10.1080/19768354.2025.2542176","DOIUrl":"10.1080/19768354.2025.2542176","url":null,"abstract":"The additional sex combs-like 1 (Asxl1) gene is a chromatin regulator involved in transcriptional activation and repression. While Asxl1 plays a crucial role in various organ development, its role in ocular development remains unclear. Here, we analyzed Asxl1 knockout (KO) mice and observed disrupted optic cup formation at embryonic day 10.5 (E10.5). RNA-seq of the E10.5 optic cup revealed dysregulation of Wnt signaling and early eye development genes. In further investigation using isolated cell from E10.5 retinal region, neuroepithelial stem cells from Asxl1 KO embryos exhibited impaired proliferation and spheroid formation. To elucidate the transcriptional mechanism by Asxl1 in optic cup formation, biochemical assays demonstrated that Asxl1 binds the LIM domain of Lhx2, facilitating repression of Wnt1, Wnt2, and Wnt8b. Following ChIP analysis showed that the gain of function of Asxl1 increased repressive histone marks (H3K27me3, H3K9me3) and reduced active marks (H3K4me3) at Lhx2-binding motifs within the cis-regulatory regions of canonical Wnt ligand genes. These findings establish Asxl1 as a key epigenetic regulator of optic cup development by modulating Lhx2-mediated Wnt signaling, providing insights into congenital eye disorders.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"488-501"},"PeriodicalIF":3.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Troponin I - a comprehensive review of its function, structure, evolution, and role in muscle diseases. 肌钙蛋白I -其功能，结构，进化和在肌肉疾病中的作用的综合综述。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-07-28 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2533821

Dongju Han, Younghyun Lim, Soah Lee, Seong-Il Eyun

{"title":"Troponin I - a comprehensive review of its function, structure, evolution, and role in muscle diseases.","authors":"Dongju Han, Younghyun Lim, Soah Lee, Seong-Il Eyun","doi":"10.1080/19768354.2025.2533821","DOIUrl":"10.1080/19768354.2025.2533821","url":null,"abstract":"The troponin complex is a critical component of thin filaments and plays an essential role in the calcium-mediated regulation of contraction and relaxation in striated muscles, including both cardiac and skeletal muscle. Troponin I, a subunit of this complex, inhibits actomyosin interactions during muscle relaxation. Its function is finely tuned by posttranslational modifications, particularly phosphorylation, which influence calcium sensitivity and actin affinity, thus impacting muscle contraction. Mutations in troponin I are closely associated with various human diseases. Specifically, several mutations in cardiac troponin I have been linked to cardiomyopathies, such as hypertrophic, dilated, and restrictive cardiomyopathies, which affect heart contractility and calcium handling. In this review, we explore the multifaceted aspects of troponin I, including its structure, functional role in muscle contraction, evolution, and the complex interactions between posttranslational modifications and genetic mutations that alter its function and contribute to disease progression.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"446-468"},"PeriodicalIF":3.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12305882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zanthoxylum schinifolium extracts enhance 3T3-L1 adipocyte differentiation via CHOP inhibition and PPARγ activation. 花椒提取物通过抑制CHOP和激活PPARγ促进3T3-L1脂肪细胞分化。

IF 3.2 2区生物学

Animal Cells and Systems Pub Date : 2025-07-25 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2536022

Jiseok Lee, Bo-Ram Kim, Kyeoungtae Park, Eunbin Kim, Jin-Woo Jeong, Jung Jin Kim, Sung-Suk Suh, Jong Bae Seo

{"title":"Zanthoxylum schinifolium extracts enhance 3T3-L1 adipocyte differentiation via CHOP inhibition and PPARγ activation.","authors":"Jiseok Lee, Bo-Ram Kim, Kyeoungtae Park, Eunbin Kim, Jin-Woo Jeong, Jung Jin Kim, Sung-Suk Suh, Jong Bae Seo","doi":"10.1080/19768354.2025.2536022","DOIUrl":"10.1080/19768354.2025.2536022","url":null,"abstract":"Zanthoxylum schinifolium (ZS), which is widely used as a seasoning and traditional medicine in East Asia, has demonstrated pharmacological potential. This study investigated the effects of the leaf and twig extracts of ZS (LZSE and TZSE, respectively), which are native to the Honam region of Korea, on adipocyte differentiation and assessed the ligand-binding energy score of their components to bind peroxisome proliferator-activated receptor gamma (PPARγ), a critical regulator of adipogenesis and metabolic health. LZSE and TZSE were prepared using 70% ethanol, and their molecular effects on adipocyte differentiation were evaluated in 3T3-L1 preadipocytes. Single compounds from the extracts were identified using UPLC-ESI-Q-TOF-MS, and their ligand-binding energy scores were calculated via in silico molecular docking studies. PPARγ activity was further confirmed through reporter assays. LZSE and TZSE significantly promoted adipocyte differentiation, as demonstrated by morphological changes and the increased mRNA and protein levels of key adipogenic and lipogenic genes, such as PPARγ and CCAAT-enhancer-binding protein alpha (C/EBPα). LZSE specifically enhanced adipogenesis without inducing cytotoxicity, attributed to the inhibition of C/EBP homologous protein (CHOP) and stimulation of mitotic expansion. Additionally, UPLC-ESI-Q-TOF-MS identified several active compounds in LZSE and TZSE, and in silico docking revealed the high binding affinity of these compounds for the full-agonist ligand-binding domain of PPARγ. LZSE and TZSE could emerge as novel antidiabetic drug candidates based on their effects on adipocyte differentiation and PPARγ activation. Furthermore, the active compounds identified in these extracts hold promise as tentative PPARγ agonists, highlighting their therapeutic potential in the treatment of metabolic disorders.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"469-487"},"PeriodicalIF":3.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis. 在爪蟾胚胎发生过程中，Prdx6通过氧化还原控制来调节体内髓细胞的发育。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-07-16 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2533823

Minjoo Kim, Hyun-Kyung Lee, Hongchan Lee, Hyun-Shik Lee

{"title":"Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis.","authors":"Minjoo Kim, Hyun-Kyung Lee, Hongchan Lee, Hyun-Shik Lee","doi":"10.1080/19768354.2025.2533823","DOIUrl":"10.1080/19768354.2025.2533823","url":null,"abstract":"Peroxiredoxin6 (Prdx6) is a bifunctional antioxidant enzyme with both peroxidase and phospholipase A₂ activities. Although its molecular roles are well established, the developmental role of Prdx6 remains poorly understood. To address this gap in the literature, this study aimed to examine the in vivo function of Prdx6 in primitive myelopoiesis using Xenopus laevis embryos. We found that prdx6 is specifically expressed in myeloid progenitors originating from the anterior ventral blood island during early embryogenesis. Knockdown of prdx6 significantly reduced the number of myeloid cells, without affecting their migration ability. Embryos depleted of prdx6 exhibited elevated levels of reactive oxygen species (ROS) and decreased cellular proliferation. Co-injection of morpholino (MO)-resistant prdx6 mRNA or treatment with N-acetylcysteine (NAC) successfully restored both ROS levels and myeloid cell numbers, suggesting that Prdx6 supports primitive myeloid cell development by maintaining redox homeostasis. These findings reveal a novel role of Prdx6 in ROS-dependent proliferation of myeloid progenitors during early vertebrate development.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"438-445"},"PeriodicalIF":2.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells. 细胞角蛋白-1对于检测内皮细胞的层状剪切应力至关重要。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-07-06 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2526426

Sunyoung Ahn, Youngsik Seo, Heonyong Park

{"title":"Cytokeratin-1 is essential for the detection of laminar shear stress in endothelial cells.","authors":"Sunyoung Ahn, Youngsik Seo, Heonyong Park","doi":"10.1080/19768354.2025.2526426","DOIUrl":"10.1080/19768354.2025.2526426","url":null,"abstract":"Endothelial cells regulate diverse vascular functions by perceiving and reacting to laminar shear stress. In this study, a novel shear-sensing receptor was identified through the use of a pro-inflammatory protein, lysyl-tRNA synthetase (KARS), which is known to be secreted from endothelial cells via autophagy. Binding assays demonstrated that cytokeratin-1 (CK1) interacts with KARS at the endothelial cell surface. Additionally, CK1 was shown to be critical for ECM-cell adhesion and endothelial sensing of shear stress by mediating interactions with laminin and integrin α6. Overexpression of CK1 results in hyperactivation of endothelial nitric oxide synthase (eNOS) in response to laminar shear stress (LSS), potentially reducing the risk of atherosclerosis. Furthermore, elevated CK1 expression significantly decreases leukocyte adhesion to endothelial cells by modulating nitric oxide production stimulated by LSS. Conversely, CK1 knockdown leads to the formation of actin fibers and diminishes LSS-induced activation of several cell signaling components. These findings indicate that CK1 is a shear-sensing receptor, providing new perspectives on the close relationship between cell-to-matrix adhesion and mechanosensing.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"426-437"},"PeriodicalIF":2.5,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Holoprosencephaly and cyclopia in bmp7b and bmpr1ba Crispant zebrafish. bmp7b和bmp1ba脆斑斑马鱼前脑畸形和独眼畸形的研究。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2025-07-01 eCollection Date: 2025-01-01 DOI: 10.1080/19768354.2025.2519018

Valentyn Kyrychenko, Philipp Rensinghoff, Johannes Bulk, Constanze Frey, Stephan Heermann

{"title":"Holoprosencephaly and cyclopia in bmp7b and bmpr1ba Crispant zebrafish.","authors":"Valentyn Kyrychenko, Philipp Rensinghoff, Johannes Bulk, Constanze Frey, Stephan Heermann","doi":"10.1080/19768354.2025.2519018","DOIUrl":"10.1080/19768354.2025.2519018","url":null,"abstract":"Holoprosencephaly (HPE) is the most frequent developmental disorder of the forebrain. In HPE, the early single anlage of the forebrain, the anterior neural plate (ANP) which encompasses the future telencephalon and eye field, fails to divide. BMP signaling and antagonism are overall important for nervous system development. The focus of this study was on the role of the ligand bmp7b and the receptor bmpr1ba during forebrain development. The zebrafish loci of bmp7b and bmpr1ba were targeted transiently with CRISPR/Ca9. Crispants for both bmp7b and bmpr1ba presented HPE and cyclopia, one central eye. Subsequently, the ANP was addressed in bmp7b Crispants. The morphology of the eye field was affected, with important markers, rx3, six3b and cxcr4a expressed condensed at the midline. Induced expression of bmp4 is also known to result in HPE. Such bmp4 induction altered the expression of bmpr1ba. Zebrafish Crispants for bmp7b and bmpr1ba can be used as a novel HPE model. A challenge in future analyses will be the penetrance of phenotypes in Crispants. The advantages are, however, that analyses can be conducted anywhere, without the need of mutant lines. One important aspect for future analysis will be the role of individual bmp ligands, receptors and antagonists in forebrain development.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":"29 1","pages":"16-27"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12217118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0