Animal Cells and Systems最新文献_第10页

Brain type of creatine kinase induces doxorubicin resistance via TGF-β signaling in MDA-MB-231 breast cancer cells. 脑型肌酸激酶通过TGF-β信号在MDA-MB-231乳腺癌症细胞中诱导阿霉素耐药性。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-08-31 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2107070

Seogho Son, Seung-Ah Yoo, KeeSoo Nam, Sunhwa Oh, Kyung-Min Lee, Jae Youn Yi, Incheol Shin

{"title":"Brain type of creatine kinase induces doxorubicin resistance via TGF-β signaling in MDA-MB-231 breast cancer cells.","authors":"Seogho Son, Seung-Ah Yoo, KeeSoo Nam, Sunhwa Oh, Kyung-Min Lee, Jae Youn Yi, Incheol Shin","doi":"10.1080/19768354.2022.2107070","DOIUrl":"https://doi.org/10.1080/19768354.2022.2107070","url":null,"abstract":"Brain type of creatine kinase (CKB) regulates energy homeostasis by reversibly transferring phosphate groups between phosphocreatine and ATP at sites of high energy demand. Several types of cancer cells exhibit upregulated CKB expression, but the function of CKB in cancer cells remains unclear. In this study, we investigated the function of CKB in breast cancer by overexpressing CKB in MDA-MB-231 cells. The overexpression of CKB did not affect cell growth rate, cell cycle distribution, ATP level or key mediators of aerobic glycolysis and lactate dehydrogenase isoform levels. Meanwhile, CKB overexpression did increase resistance to doxorubicin. TGF-β-induced Smad phosphorylation and Smad-dependent transcriptional activity were significantly up-regulated by CKB expression without changes in inhibitory Smad protein levels. Moreover, treatment with TGF-β considerably enhanced cell viability during doxorubicin treatment and decreased doxorubicin-induced apoptosis in CKB-expressing MDA-MB-231 cells compared to control cells. These results suggest that CKB attenuates doxorubicin-induced apoptosis and potentiates resistance to doxorubicin by enhancing TGF-β signaling in MDA-MB-231 cells.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40652044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective effects of tannic acid in the postischemic brain via direct chelation of Zn². 单宁酸通过直接螯合Zn2在脑缺血后的神经保护作用。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-08-19 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2113915

Seung Woo Kim, Da Bin Kim, Hong Seok Kim

{"title":"Neuroprotective effects of tannic acid in the postischemic brain via direct chelation of Zn2.","authors":"Seung Woo Kim, Da Bin Kim, Hong Seok Kim","doi":"10.1080/19768354.2022.2113915","DOIUrl":"https://doi.org/10.1080/19768354.2022.2113915","url":null,"abstract":"Tannic acid (TA) is a polyphenolic compound that exerts protective effects under pathological conditions. The diverse mechanisms of TA can exert beneficial anti-oxidative, anti-inflammatory, and anti-cancer effects. Herein, we reported that TA affords robust neuroprotection in an animal model of stroke (transient middle cerebral artery occlusion; tMCAO) and exhibits Zn2+-chelating and anti-oxidative effects in primary cortical neurons. Following tMCAO induction, intravenous administration of TA (5 mg/kg) suppressed infarct formation by 32.9 ± 16.2% when compared with tMCAO control animals, improving neurological deficits and motor function. We compared the chelation activity under several ionic conditions and observed that TA showed better Zn2+ chelation than Cu2+. Furthermore, TA markedly decreased lactate dehydrogenase release following acute Zn2+ treatment and subsequently reduced the expression of p67 (a cytosolic component of NADPH oxidase), indicating the potential mechanism underlying TA-mediated Zn2+ chelation and anti-oxidative effects in primary cortical neurons. These findings suggest that anti-Zn2+ toxicity and anti-oxidative effects participate in the TA-mediated neuroprotective effects in the postischemic brain.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40334172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Luteolin reduces fear, anxiety, and depression in rats with post-traumatic stress disorder. 木犀草素减轻创伤后应激障碍大鼠的恐惧、焦虑和抑郁。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-08-19 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2104925

Bongjun Sur, Bombi Lee

{"title":"Luteolin reduces fear, anxiety, and depression in rats with post-traumatic stress disorder.","authors":"Bongjun Sur, Bombi Lee","doi":"10.1080/19768354.2022.2104925","DOIUrl":"https://doi.org/10.1080/19768354.2022.2104925","url":null,"abstract":"Exposure to severe stress can lead to the development of neuropsychiatric disorders, including post-traumatic stress disorder (PTSD). The cause of PTSD is dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis and an imbalance of monoamines. Fruits and vegetables contain large amounts of luteolin (LU; 3',4',5,7-tetrahydroxylflavone), which has various pharmacological activities such as anti-inflammatory, antioxidant, and anti-allergic effects. We investigated the effects of LU on fear, depression, and anxiety following monoamine imbalance and hyperactivation of the HPA axis in rats exposed to single prolonged stress (SPS). Male rats were dosed with LU (10 and 20 mg/kg) once daily for 14 days after exposure to SPS. Administration of LU reduced fear freezing responses to extinction recall and depression- and anxiety-like behaviors, and suppressed increases in plasma corticosterone and adrenocorticotropic hormone levels. Also, administration of LU restored the increased norepinephrine and decreased serotonin levels in the structures within the fear circuit, medial prefrontal cortex, and hippocampus. Our results showed that administration of LU improved freezing behavior according in a situation-dependent manner, and showed anti-depressant and anxiolytic effects. Thus, LU may be a useful therapeutic agent to prevent traumatic stress such as PTSD.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5a/26/TACS_26_2104925.PMC9423864.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40334173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Neuro-immune interactions at single-cell resolution in neurodevelopmental, infectious, and neurodegenerative diseases. 神经发育、感染性和神经退行性疾病中单细胞分辨率的神经免疫相互作用。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-08-11 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2110937

Hyun Jung Park, Hosung Jung

引用次数: 3

The mixture of Agrimonia pilosa Ledeb. and Salvia miltiorrhiza Bunge. extract produces analgesic and anti-inflammatory effects in a collagen-induced arthritis mouse model. 沙棘草的混合物。和丹参。提取物在胶原诱导的关节炎小鼠模型中具有镇痛和抗炎作用。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-08-08 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2106302

Jing Hui Feng, Jeon Sub Jung, Seung Hwan Hwang, Soo Kyeong Lee, Sang Youn Lee, Youn Gil Kwak, Doo-Ho Kim, Chu-Youn Song, Min Jung Kim, Hong Won Suh, Sung Chan Kim, Soon Sung Lim

{"title":"The mixture of Agrimonia pilosa Ledeb. and Salvia miltiorrhiza Bunge. extract produces analgesic and anti-inflammatory effects in a collagen-induced arthritis mouse model.","authors":"Jing Hui Feng, Jeon Sub Jung, Seung Hwan Hwang, Soo Kyeong Lee, Sang Youn Lee, Youn Gil Kwak, Doo-Ho Kim, Chu-Youn Song, Min Jung Kim, Hong Won Suh, Sung Chan Kim, Soon Sung Lim","doi":"10.1080/19768354.2022.2106302","DOIUrl":"https://doi.org/10.1080/19768354.2022.2106302","url":null,"abstract":"Pain and inflammation typically manifest in patients with arthritis. It is now widely known that Agrimonia pilosa Ledeb (AP) and Salvia miltiorrhiza Bunge (SM) exert anti-inflammatory and antinociceptive effects. We have previously reported that the mixture extract (ME) from AP and SM produces antinociceptive and anti-inflammatory effects in gout arthritis and monoiodoacetate (MIA)-induced arthritis models. In the present study, we assessed the antinociceptive and anti-inflammatory effects on the collagen-induced arthritis (CIA) model. The antinociceptive effects in mice were measured using the von Frey test. ME administered once or for one week (once per day) once, and one-week reduced the pain in a dose-dependent manner (from 50 to 100 mg/kg) in the CIA-induced osteoarthritis (OA) model. ME treatment also reduced tumor necrosis factor (TNF)-α and C-reactive protein (CRP) levels in plasma and ankle tissues. Furthermore, COX-1, COX-2, NF-κB, TNF-α, and IL-6 expressions were attenuated after ME treatment. In most experiments, the antinociceptive and anti-inflammatory effects induced by ME treatment were almost equal to or slightly better than those induced by Perna canaliculus (PC) treatment, which was used as a positive control. Our results suggest that ME possesses antinociceptive and anti-inflammatory effects, indicating its potential as a therapeutic agent for arthritis treatment.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40334622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Restoration of NAD⁺ homeostasis protects C2C12 myoblasts and mouse levator ani muscle from mechanical stress-induced damage. 恢复NAD+稳态可保护C2C12成肌细胞和小鼠提肛肌免受机械应力引起的损伤。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-08-03 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2106303

Guotao Huang, Yong He, Li Hong, Min Zhou, Xiaohu Zuo, Zhihan Zhao

{"title":"Restoration of NAD+ homeostasis protects C2C12 myoblasts and mouse levator ani muscle from mechanical stress-induced damage.","authors":"Guotao Huang, Yong He, Li Hong, Min Zhou, Xiaohu Zuo, Zhihan Zhao","doi":"10.1080/19768354.2022.2106303","DOIUrl":"https://doi.org/10.1080/19768354.2022.2106303","url":null,"abstract":"Excessive mechanical traction damages the levator ani muscle (LAM), increasing the incidence of pelvic floor dysfunction (PFD). In this study, we explored the effects of oxidized nicotinamide adenine dinucleotide (NAD+) on the damage to both muscle cells and LAM tissue induced by mechanical stress (MS) at the cellular and animal levels. The cell damage model was established using a four-point bending system. The LAM damage model was established using vaginal distention and traction. Exogenous addition of PJ34, an inhibitor of poly (ADP-ribose) polymerase-1 (PARP-1), and the nicotinamide mononucleotide (NMN) precursor of NAD+ increased NAD+ levels. ATP content and mitochondrial membrane potential were measured to assess mitochondrial function. NAD+ levels, cell viability, and PARP-1 activity were detected using commercial kits. DNA damage in cells was detected with immunofluorescence staining, and LAM damage was detected with tissue TUNEL staining. PARP-1 activity and DNA damage of LAM were detected by immunohistochemistry. A small amount of DNA damage and PARP-1 activation did not affect NAD+ levels, while excessive DNA damage and PARP-1 activation led to an imbalance of NAD+ homeostasis. Furthermore, increasing NAD+ levels in vivo and in vitro could rescue mitochondrial dysfunction and damage to both muscle cells and LAM tissue induced by MS. In conclusion, MS can induce damage to both C2C12 cells and LAM tissue. Restoring NAD+ homeostasis can rescue this damage by improving mitochondrial function.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40334174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Melittin-derived peptides exhibit variations in cytotoxicity and antioxidant, anti-inflammatory and allergenic activities. 蜂毒蛋白衍生的多肽在细胞毒性、抗氧化、抗炎和致敏活性方面表现出差异。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-07-18 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2099971

Haesoo Jung, Yong Soo Kim, Da-Min Jung, Kyeong-Seob Lee, Jung-Min Lee, Kee K Kim

{"title":"Melittin-derived peptides exhibit variations in cytotoxicity and antioxidant, anti-inflammatory and allergenic activities.","authors":"Haesoo Jung, Yong Soo Kim, Da-Min Jung, Kyeong-Seob Lee, Jung-Min Lee, Kee K Kim","doi":"10.1080/19768354.2022.2099971","DOIUrl":"https://doi.org/10.1080/19768354.2022.2099971","url":null,"abstract":"Melittin is a major component of bee venom; it is widely used in traditional medicine because of its therapeutic effects, such as anti-inflammatory effects. However, melittin has limited medical applications owing to its adverse effects, such as high cytotoxicity. In this study, we investigated the physiological activities of various hydrolyzed melittin-derived peptides to eliminate the cytotoxicity of melittin and enhance its efficacy. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay confirmed that melittin-derived peptides showed antioxidant activity comparable to that of melittin. Moreover, unlike melittin, which showed high cytotoxicity in the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay, the melittin-derived peptides showed negligible cytotoxicity. Among the melittin-derived peptides, the peptide composed of sequence TTGLPALISWIKRKRQQ (P1) showed inhibitory effects on the mRNA expression of inflammatory cytokines and phosphorylation of IκBα, similar to the effects of melittin in RAW 264.7 cells. Degranulation of RBL-2H3 cells was analyzed using a β-hexosaminidase release assay to confirm the allergenic activity of melittin and P1, which showed remarkably reduced allergenicity of P1 compared to that of melittin. These results indicate that P1 maintained the anti-inflammatory effects of melittin while reducing its cytotoxicity and allergic reactions. In conclusion, the melittin-derived peptide P1 efficiently decreased the adverse effects while maintaining the beneficial effects of melittin, making it suitable for therapeutic applications.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40334623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming. 胰腺星状细胞分泌的CXCL12通过增强糖酵解重编程加速胰腺癌对吉西他滨的耐药。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-07-04 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2091019

Xiangyu Lu, Yilei Wu, Rui Cao, Xiaojiong Yu, Jun Gong

{"title":"CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming.","authors":"Xiangyu Lu, Yilei Wu, Rui Cao, Xiaojiong Yu, Jun Gong","doi":"10.1080/19768354.2022.2091019","DOIUrl":"https://doi.org/10.1080/19768354.2022.2091019","url":null,"abstract":"Pancreatic stellate cells (PSCs) are the primary cell components of pancreatic cancer (PC) and are involved in tumor growth, metastasis and resistance. However, the role and the mechanism of PSCs in gemcitabine (GEM) resistance to PC still need more investigation. We found that CXCL12 mRNA and secreted CXCL12 protein were higher in PSCs after GEM treatment. The conditioned medium (CM) from GEM-treated PSCs reduced the GEM sensitivity of PC cells. Blocking of CXCL12 in CM by anti-CXCL12 antibody partly restored the GEM sensitivity of PC cells. Blocking of CXCL12 decreased glucose consumption, lactate production, ECAR, and glycolysis-related gene expression in PC cells. The PI3K/AKT/mTOR pathway was activated by the binding of CXCL12 and CXCR4. Moreover, CXCR4 mRNA and protein expressions in PC cells were increased after GEM treatment. Our results indicated the cross-talk between PSCs and PC cells during GEM chemotherapy. CXCL12 secreted by PSCs reduces GEM sensitivity of PC cells by binding to CXCR4 and activating PI3K/AKT/mTOR-glycolysis pathway in PC. Our findings would lay the foundation for solving GEM resistance in PC.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1d/b3/TACS_26_2091019.PMC9423839.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40334624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

ECM1 is associated with endocrine resistance in ER⁺ breast cancers. ECM1与雌激素受体阳性乳腺癌的内分泌抵抗有关。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-06-22 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2083235

Tae Won Lee, Kyung-Min Lee

{"title":"ECM1 is associated with endocrine resistance in ER+ breast cancers.","authors":"Tae Won Lee, Kyung-Min Lee","doi":"10.1080/19768354.2022.2083235","DOIUrl":"https://doi.org/10.1080/19768354.2022.2083235","url":null,"abstract":"Extracellular matrix protein 1 (ECM1) is associated with a poor prognosis of breast cancers. However, the role of ECM1 with endocrine resistance in estrogen receptor-positive (ER+) breast cancers has not been elucidated yet. We show that ECM1 promotes endocrine resistance in ER+ breast cancers. ECM1 is overexpressed in luminal breast cancer patients compared to the basal type of breast cancer. Significantly, higher expression of ECM1 is associated with poor response to endocrine therapies in luminal B breast cancer patients. We found that ECM1 is upregulated in CAMA1 and MDA-MB-361 cells grown in long-term estrogen-deprived (LTED) conditions. Moreover, the ablation of ECM1 significantly inhibited the proliferation of CAMA1 LTED and MDA-MB-361 LTED cells. Finally, an interrogation of a dataset containing transcriptome and proteome of breast cancer cell lines revealed that the level of ECM1 mRNA is positively correlated with that of phosphorylated Src. Based on these findings, we strongly suggest that ECM1 significantly contributes to the acquisition of endocrine resistance in ER+ breast cancers by the activation of Src.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40468853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mangifera Indica leaf extracts promote hair growth via activation of Wnt signaling pathway in human dermal papilla cells. 芒果叶提取物通过激活人真皮乳头细胞的 Wnt 信号通路促进毛发生长。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2022-06-11 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2085790

Haesoo Jung, Da-Min Jung, Sang-Soo Lee, Eun-Mi Kim, Kyungah Yoon, Kee K Kim

{"title":"Mangifera Indica leaf extracts promote hair growth via activation of Wnt signaling pathway in human dermal papilla cells.","authors":"Haesoo Jung, Da-Min Jung, Sang-Soo Lee, Eun-Mi Kim, Kyungah Yoon, Kee K Kim","doi":"10.1080/19768354.2022.2085790","DOIUrl":"10.1080/19768354.2022.2085790","url":null,"abstract":"The crosstalk between androgens and Wnt signaling pathways is critical in the hair growth cycle. Therefore, natural products that target these two pathways for the inhibition of hair loss are sought after. In this study, we investigated the effect of water extracts of Mangifera indica leaves (WEML) on hair growth. WEML treatment significantly reduced the expression levels of both dickkopf-1 (DKK1) and type 2 5α-reductase (SRD5A2) involved in Wnt signal suppression activity and dihydrotestosterone (DHT) synthesis, respectively, in human follicle dermal papilla cells (HFDP). In addition, WEML treatment effectively upregulated Wnt target genes and downregulated DKK1 expression that was increased by DHT treatment. Degranulation analysis in rat basophilic leukemia mast cell line (RBL-2H3) using β-hexosaminidase release assay confirmed that WEML did not exhibit allergenic activity. Furthermore, hair growth was significantly enhanced in in vivo mice model treated with WEML. These results suggest that M. indica leave extract contains bioactive materials that can be used to treat hair loss.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40468856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0