Animal Cells and Systems最新文献_第9页

Crosstalk between endoplasmic reticulum stress response and autophagy in human diseases. 人类疾病中内质网应激反应与自噬之间的串扰。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2023-01-01 DOI: 10.1080/19768354.2023.2181217

Junhee Kwon, Jihyun Kim, Keun Il Kim

{"title":"Crosstalk between endoplasmic reticulum stress response and autophagy in human diseases.","authors":"Junhee Kwon, Jihyun Kim, Keun Il Kim","doi":"10.1080/19768354.2023.2181217","DOIUrl":"https://doi.org/10.1080/19768354.2023.2181217","url":null,"abstract":"Cells activate protective mechanisms to overcome stressful conditions that threaten cellular homeostasis, including imbalances in calcium, redox, and nutrient levels. Endoplasmic reticulum (ER) stress activates an intracellular signaling pathway, known as the unfolded protein response (UPR), to mitigate such circumstances and protect cells. Although ER stress is sometimes a negative regulator of autophagy, UPR induced by ER stress typically activates autophagy, a self-degradative pathway that further supports its cytoprotective role. Sustained activation of ER stress and autophagy is known to trigger cell death and is considered a therapeutic target for certain diseases. However, ER stress-induced autophagy can also lead to treatment resistance in cancer and exacerbation of certain diseases. Since the ER stress response and autophagy affect each other, and the degree of their activation is closely related to various diseases, understanding their relationship is very important. In this review, we summarize the current understanding of two fundamental cellular stress responses, the ER stress response and autophagy, and their crosstalk under pathological conditions to help develop therapies for inflammatory diseases, neurodegenerative disorders, and cancer.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9970256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10812289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Emerging roles of polyunsaturated fatty acid synthesis pathway in colorectal cancer. 多不饱和脂肪酸合成途径在结直肠癌中的新作用。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2023-01-01 DOI: 10.1080/19768354.2023.2189933

Young-Ah Moon

{"title":"Emerging roles of polyunsaturated fatty acid synthesis pathway in colorectal cancer.","authors":"Young-Ah Moon","doi":"10.1080/19768354.2023.2189933","DOIUrl":"https://doi.org/10.1080/19768354.2023.2189933","url":null,"abstract":"The development of colorectal cancer typically involves the accumulated influences of genetic alterations, medical issues, lifestyle, and diet. Dietary fatty acids appear to affect the tumorigenesis and progression of colorectal cancer. Despite conflicting results, the current consensus on the effects of very long-chain polyunsaturated fatty acids on colorectal cancer is that low levels of eicosapentaenoic acid and docosahexaenoic acid, and high levels of arachidonic acid are associated with an increased risk of colorectal cancer. Altered levels of arachidonic acid in membrane phospholipids can change the levels of prostaglandin E2, which affect the biological activities of cancer cells in multiple stages. Arachidonic acid and other very long-chain polyunsaturated fatty acids can affect tumorigenesis in prostaglandin E2-independent manners as well, including stabilization of β-catenine, ferroptosis, ROS generation, regulation of transcription factors, and de novo lipogenesis. Recent studies have revealed an association between the activities of enzymes synthesizing very long-chain polyunsaturated fatty acids and tumorigenesis and cancer progression, although the mechanisms are still unknown. In this study, PUFA effects on tumorigenesis, the endogenous very long-chain polyunsaturated fatty acid synthesis pathway, metabolites of arachidonic acid and their effects on tumorigenesis and progression of CRC, and current knowledge that supports the association of the enzymes involved in the polyunsaturated fatty acid synthesis pathway with colorectal cancer tumorigenesis and progression are reviewed.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9561614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloromethylisothiazolinone induces ER stress-induced stress granule formation in human keratinocytes. 氯甲基异噻唑啉酮诱导内质网应激诱导的人角化细胞应激颗粒形成。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2023-01-01 DOI: 10.1080/19768354.2023.2250852

Da-Min Jung, Kee K Kim, Eun-Mi Kim

{"title":"Chloromethylisothiazolinone induces ER stress-induced stress granule formation in human keratinocytes.","authors":"Da-Min Jung, Kee K Kim, Eun-Mi Kim","doi":"10.1080/19768354.2023.2250852","DOIUrl":"https://doi.org/10.1080/19768354.2023.2250852","url":null,"abstract":"Chloromethylisothiazolinone (CMIT), a humidifier disinfectant, is known to be toxic to the respiratory system. While the toxic effect of CMIT on the lungs has been widely investigated, its effect on the skin is well unknown. In this study, we examined stress granule (SG) formation to investigate the cytotoxic effects of CMIT on human keratinocytes. We assessed the viability of the cells following CMIT exposure and performed immunofluorescence microscopy and immunoblot analyses to determine SG formation and downstream pathways. The IC50 values in human keratinocyte HaCaT cells after CMIT exposure for 1 and 24 h were 11 and 8 μg/mL, respectively, showing no significant difference. As determined using immunofluorescence microscopy, SG formation was effectively induced after CMIT exposure. Moreover, the phosphorylation of eukaryotic initiation factor-2α (eIF2α), a translation initiation factor, and protein kinase R-like endoplasmic reticulum (ER) kinase, which plays a role in the ER stress-mediated eIF2α phosphorylation, was confirmed by CMIT exposure. These results suggest that exposure to CMIT can have detrimental effects on the skin, even briefly, by inducing SG formation through ER stress in keratinocytes.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10197251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rg3 attenuates pulmonary fibrosis by inhibiting endothelial to mesenchymal transition. 人参皂苷Rg3通过抑制内皮细胞向间质细胞的转变而减轻肺纤维化。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2023-01-01 DOI: 10.1080/19768354.2023.2244549

Eunsik Yun, Byung Su Kwon, Jongmin Kim, Aram Lee

{"title":"Ginsenoside Rg3 attenuates pulmonary fibrosis by inhibiting endothelial to mesenchymal transition.","authors":"Eunsik Yun, Byung Su Kwon, Jongmin Kim, Aram Lee","doi":"10.1080/19768354.2023.2244549","DOIUrl":"https://doi.org/10.1080/19768354.2023.2244549","url":null,"abstract":"Pulmonary fibrosis (PF) is a progressive and chronic lung disease characterized by excessive extracellular matrix (ECM) deposition and fibroblast proliferation. Endothelial-to-mesenchymal transition (EndMT) serves as a source of fibroblasts and contributes to PF progression. Ginsenoside Rg3 (Rg3), a steroidal saponin extracted from ginseng, is known to have pharmacological effects on vascular diseases. We have previously demonstrated that Rg3 inhibits EndMT and prevents endothelial dysfunction. Thus, we hypothesized that Rg3 may be a potential therapeutic agent for PF-targeting EndMT. EndMT occurs in the lung tissue of a bleomycin-induced PF mouse model, which was confirmed by co-staining of endothelial and mesenchymal markers in the pulmonary vasculature and changes in the expression of these markers. Rg3 administration decreased EndMT and suppressed PF development. We also examined the effect of Rg3 in an in vitro EndMT model induced by co-treatment with TGF-β2 and IL-1β. Rg3 treatment alleviated the characteristics of EndMT such as spindle-shaped morphological changes, EndMT marker expression changes, Dil-Ac-LDL uptake and migratory properties. In addition, we demonstrated the mechanism by which Rg3 inhibits EndMT by regulating the Smad2/3 signaling pathway. Collectively, Rg3 can be a potential therapeutic agent for PF using the EndMT inhibition strategy, furthermore, it can be considered Rg3 as a therapeutic candidate for various EndMT-associated vascular diseases.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/30/TACS_27_2244549.PMC10405771.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10198190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium. 利用含抗坏血酸的成熟培养基从人造血干细胞中有效地衍生心室心肌细胞。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2023-01-01 DOI: 10.1080/19768354.2023.2189932

Ji-Eun Kim, Eun-Mi Kim, Hyang-Ae Lee, Ki-Suk Kim

{"title":"Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium.","authors":"Ji-Eun Kim, Eun-Mi Kim, Hyang-Ae Lee, Ki-Suk Kim","doi":"10.1080/19768354.2023.2189932","DOIUrl":"https://doi.org/10.1080/19768354.2023.2189932","url":null,"abstract":"Cardiomyocytes derived from human pluripotent stem cells (hPSCs) can be used in various applications including disease modeling, drug safety screening, and novel cell-based cardiac therapies. Here, we report an optimized selection and maturation method to induce maturation of cardiomyocytes into a specific subtype after differentiation driven by the regulation of Wnt signaling. The medium used to optimize selection and maturation was in a glucose starvation conditions, supplemented with either a nutrition complex or ascorbic acid. Following optimized selection and maturation, more cardiac Troponin T (cTnT)-positive cardiomyocytes were detected using albumin and ascorbic acid than B27. In addition, ascorbic acid enriched maturation of ventricular cardiomyocytes. We compared cardiomyocyte-specific gene expression patterns under different selection and maturation conditions by next-generation sequencing (NGS) analysis. Our optimized conditions will enable simple and efficient maturation and specification of the desired cardiomyocyte subtype, facilitating both biomedical research and clinical applications.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9231034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish. 嘌呤霉素诱导的成年斑马鱼肾损伤和随后的再生。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2023-01-01 DOI: 10.1080/19768354.2023.2203211

Soonil Koun, Hye-Jin Park, Su-Min Jung, Jin Joo Cha, Dae Ryong Cha, Young Sun Kang

{"title":"Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish.","authors":"Soonil Koun, Hye-Jin Park, Su-Min Jung, Jin Joo Cha, Dae Ryong Cha, Young Sun Kang","doi":"10.1080/19768354.2023.2203211","DOIUrl":"https://doi.org/10.1080/19768354.2023.2203211","url":null,"abstract":"Puromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury and subsequent regeneration following puromycin treatment in adult zebrafish. We intraperitoneally injected 100 μg of puromycin into zebrafish; sacrificed them at 1, 3, 5, 7, or 14 days post-injection (dpi); and examined the morphological, functional, and molecular changes in the kidney. Puromycin-treated zebrafish presented more rapid clearance of rhodamine dextran than control animals. Morphological changes were observed immediately after the puromycin injection (1-7 dpi) and had recovered by 14 dpi. The mRNA production of lhx1a, a renal progenitor marker, increased during recovery from kidney injury. Levels of NFκB, TNFα, Nampt, and p-ERK increased significantly during nephron injury and regeneration, and Sirt1, FOXO1, pax2, and wt1b showed an increasing tendency. However, TGF-β1 and smad5 production did not show any changes after puromycin treatment. This study provides evidence that puromycin-induced injury in adult zebrafish kidneys is a potential tool for evaluating the mechanism of nephron injury and subsequent regeneration.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/17/TACS_27_2203211.PMC10120544.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive peptides in the pancreatin-hydrolysates of whey protein support cell proliferation and scavenge reactive oxygen species. 乳清蛋白的胰水解产物中的生物活性肽支持细胞增殖和清除活性氧。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-10-11 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2130425

Haesoo Jung, Damin Jung, Jaehoon Lee, Woojin Ki, Jung-Min Lee, Eun-Mi Kim, Myoung Soo Nam, Kee K Kim

{"title":"Bioactive peptides in the pancreatin-hydrolysates of whey protein support cell proliferation and scavenge reactive oxygen species.","authors":"Haesoo Jung, Damin Jung, Jaehoon Lee, Woojin Ki, Jung-Min Lee, Eun-Mi Kim, Myoung Soo Nam, Kee K Kim","doi":"10.1080/19768354.2022.2130425","DOIUrl":"https://doi.org/10.1080/19768354.2022.2130425","url":null,"abstract":"Whey protein (WP) in milk shows physiologically active functions such as cholesterol control and immune system strengthening. In this study, we performed hydrolysis and peptide polarity fractionation to enhance the efficacy and diversity of its physiological activities, using the digesting enzyme, pancreatin. Our results indicate that hydrolysis significantly increased the cell proliferation of the WP fractions, with the lower-polarity fractions showing greater efficacy in this regard. Our results indicate that hydrolysis significantly increases cell proliferation of the WP fractions. Additionally, we confirmed differences in the antioxidant activity of the WP fractions as a function of polarity was confirmed via scavenging 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay in vitro. WP itself did not show anti-inflammatory efficacy. However, all the hydrolyzed fractions downregulated the mRNA expression levels of inflammatory cytokines in all treated cell lines and, based on a senescence-associated (SA)-β-galactosidase assay, the fraction with the lowest polarity (F6) inhibited cellular senescence to the greatest extent. Furthermore, we identified the peptide sequences with various physiological activities from whey protein hydrolysates through mass spectrometry. Taken together, our results indicate that the fractionation of WP via hydrolysis generates novel functions including promoting cellular cell proliferation, anti-inflammatory effects, and enhancing antioxidant and anti-cellular senescence.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40652045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Dicarbonyl/L-xylulose reductase (DCXR) producing xylitol regulates egg retention through osmolality control in Caenorhabditis elegans. 产生木糖醇的二羰基/L-木糖还原酶（DCXR）通过渗透压控制调节秀丽隐杆线虫的卵保留率。

IF 2.5 2区生物学

Animal Cells and Systems Pub Date : 2022-10-06 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2126886

Yuh-Nam Kim, Seung Hyun Kim, Le Tho Son, Joohong Ahnn, Sun-Kyung Lee

{"title":"Dicarbonyl/L-xylulose reductase (DCXR) producing xylitol regulates egg retention through osmolality control in Caenorhabditis elegans.","authors":"Yuh-Nam Kim, Seung Hyun Kim, Le Tho Son, Joohong Ahnn, Sun-Kyung Lee","doi":"10.1080/19768354.2022.2126886","DOIUrl":"10.1080/19768354.2022.2126886","url":null,"abstract":"To support life, the osmolality of the cellular fluid is tightly regulated by various means, including osmolyte control. Dicarbonyl/L-xylulose reductase (DCXR) is a highly conserved enzyme reducing L-xylulose to xylitol, which serves as an effective osmolyte in various mammalian and human tissues such as lung epithelium, sperm, and lens. DHS-21 is the only DCXR ortholog in Caenorhabditis elegans, and DCXR null mutant worms accumulate eggs in the uterus. However, it has been unknown how and why the mutant worms impair egg retention. In this study, we tested whether the egg-retention in dhs-21 (jh129), the DCXR null mutant worm, is sensitive to changes in osmolarity. Low osmolality reverted the egg retention phenotype of dhs-21(jh129), while high osmolarity aggravated it. Also, knock-down of either one of osr-1, osm-7, or osm-11, osmoregulatory genes, also rescued egg-retention phenotypes of the null mutants. The study indicates that DCXR functions in fluid homeostasis by regulating cellular osmolality in C. elegans and provides insights into DCXR-involved clinical conditions, such as congenital cataracts and malfunctioning lung and kidney.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40654003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action. 雌二醇治疗增加了肥胖去卵巢小鼠的m2样内脏脂肪组织巨噬细胞，无论其是否有厌食作用。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-09-29 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2128871

Kyeong-Dae Kim, Jeong Min Choe, Soomin Myoung, Seung Hyun Lee, Minkyu Kim, Jae-Hoon Choi, Hyun Tae Park

{"title":"Estradiol treatment increases M2-like visceral adipose tissue macrophages in obese ovariectomized mice regardless of its anorectic action.","authors":"Kyeong-Dae Kim, Jeong Min Choe, Soomin Myoung, Seung Hyun Lee, Minkyu Kim, Jae-Hoon Choi, Hyun Tae Park","doi":"10.1080/19768354.2022.2128871","DOIUrl":"https://doi.org/10.1080/19768354.2022.2128871","url":null,"abstract":"Estradiol (E2) treatment has been known to induce changes in food intake, energy expenditure, and weight gain. However, its direct effects on adipose tissue macrophages (ATM) in vivo are not fully understood. Thus, we aimed to explore this aspect at cellular and molecular levels in ovariectomized obese mice. We examined the changes in ATMs after eight weeks of a high-fat diet (HFD) in male, female, and ovariectomized (OVX) mice. After eight weeks, osmotic pumps were inserted into OVX mice to provide two weeks of E2 treatment. We additionally set up a vehicle Pair-Fed (PF) control group that supplied the same amount of HFD consumed by the E2-treated group. We then investigated the in vivo phenotypic changes of visceral adipose tissue (VAT) macrophages. The percentage of M1-like ATMs decreased by the anorectic effect of E2, while M2-like ATMs increased regardless of the anorexia. E2 treatment increased the expression of anti-inflammatory genes but decreased pro-inflammatory genes in VAT. Monocyte recruitment and local proliferation contributed to M2-like ATMs. Furthermore, M2-like phenotypes were induced by E2 treatment in human macrophages. E2 treatment increases M2-like macrophages and improves the tissue milieu of VAT regardless of the anorectic reaction of E2.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d6/16/TACS_26_2128871.PMC9586618.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40652043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular identification of archaic bones as a native Korean black bear: implications for the ongoing bear restoration program. 韩国本土黑熊古骨的分子鉴定:对正在进行的熊恢复计划的启示。

IF 2.9 2区生物学

Animal Cells and Systems Pub Date : 2022-09-20 eCollection Date: 2022-01-01 DOI: 10.1080/19768354.2022.2112755

Jee Yun Hyun, Tae-Wook Kim, Puneet Pandey, Kyung Seok Kim, Seung-Jun Jeong, Jae-Ku Kang, Dal-Yong Kong, Seung-Ho Jung, Ho-Kweon Jeong, Sang-Hoon Han, Sang-Hyun Han, Hang Lee

{"title":"Molecular identification of archaic bones as a native Korean black bear: implications for the ongoing bear restoration program.","authors":"Jee Yun Hyun, Tae-Wook Kim, Puneet Pandey, Kyung Seok Kim, Seung-Jun Jeong, Jae-Ku Kang, Dal-Yong Kong, Seung-Ho Jung, Ho-Kweon Jeong, Sang-Hoon Han, Sang-Hyun Han, Hang Lee","doi":"10.1080/19768354.2022.2112755","DOIUrl":"https://doi.org/10.1080/19768354.2022.2112755","url":null,"abstract":"The genetic investigation of the archeological or museum samples, including endangered species, provides vital information necessary to plan, implement, and revisit conservation strategies. In South Korea, the Asian black bear went almost extinct in wild by 2002, without leaving any authentic specimens representing the native population. Recently researchers found a set of animal bones in a natural cave in Mt. Taebaek (South Korea), suspected to be of a bear. In the present study, we undertook a molecular investigation and radiocarbon dating to establish the species' identity, phylogenetic position, and approximate age of the recovered specimen. The genetic investigation (CytB, COI, D-loop, SRY, and ZFX-ZFY) identified the sample as a male Asian black bear with close phylogenetic affinity with Northeast Asian bears. Radiocarbon dating estimated the bones to be aged 1800-1942 calAD. These findings indicate that the bone specimens found in the natural cave in Mt. Taebaek were from an individual that naturally inhabited South Korea long before the importing of farm bears (the 1980s) and initiation of wild population restoration (2004). The present study provides the first genetic information record of the native South Korean black bear. Our findings reaffirm the appropriateness of the ongoing bear restoration program in South Korea, with the reintroduction of individuals from North Korea and Russia.","PeriodicalId":7804,"journal":{"name":"Animal Cells and Systems","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40654002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0