Archives of biochemistry and biophysics最新文献

{"title":"The effect of omecamtiv mecarbil on actin-myosin interaction in the disused rat soleus muscle","authors":"I.K. Potoskueva ,&nbsp;O.P. Gerzen ,&nbsp;A.E. Tzybina ,&nbsp;V.O. Votinova ,&nbsp;M.V. Zhigulina ,&nbsp;K.V. Sergeeva ,&nbsp;S.A. Tyganov ,&nbsp;B.S. Shenkman ,&nbsp;L.V. Nikitina","doi":"10.1016/j.abb.2025.110442","DOIUrl":"10.1016/j.abb.2025.110442","url":null,"abstract":"<div><div>Preventing muscle atrophy caused by disuse is a major concern in space, clinical, and rehabilitation medicine. This study aimed to attenuate the impact of disuse and support muscle function during hindlimb unloading using the β-myosin activator omecamtiv mecarbil (OM). We obtained soleus muscle myosin from rats in control, control with 10-day OM supplementation, hindlimb-unloaded, and hindlimb-unloaded with 10-day OM supplementation (prolonged treatment). To examine the direct effect of OM (direct treatment) on myosin from all groups, we added it to myosin in the flow cell at a concentration of 1 μM. Using an <em>in vitro</em> motility assay, we examined the sliding velocity of actin filaments and regulated thin filaments over soleus muscle myosin, the fraction of motile filaments, calcium sensitivity and Hill coefficient in the “<em>p</em>Ca-velocity” and “<em>p</em>Ca-fraction of motile filaments” relationships, relative force. Hindlimb unloading resulted in a slow-to-fast shift in the content of myosin heavy and light chains isoforms, an increased sliding velocity of actin filaments and regulated thin filaments over myosin. 10-day OM supplementation decreased the sliding velocity of actin filaments and regulated thin filaments over myosin slightly increasing calcium sensitivity in healthy rats and prevented the increase in the velocity caused by disuse without altering relative force, myosin isoform content. Direct treatment reduced the sliding velocity of actin filaments and regulated thin filaments over myosin while enhancing calcium sensitivity and relative force in all studied groups. Thus, both direct and prolonged OM treatment mitigated the effects of disuse on the functional characteristics of soleus muscle myosin.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110442"},"PeriodicalIF":3.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coenzyme Q10 alleviates oxidative stress, inflammation and fibrosis via activation of TGFβ1/TNF-α in FCA-Salt hypertensive rats","authors":"Nusrat Hossain,&nbsp;Sadia Shabnam,&nbsp;Tushar Emran,&nbsp;Zahidul Islam Zahid,&nbsp;Shaiful Alam,&nbsp;Asim Kumar Bepari,&nbsp;G.M. Sayedur Rahman,&nbsp;Hasan Mahmud Reza","doi":"10.1016/j.abb.2025.110444","DOIUrl":"10.1016/j.abb.2025.110444","url":null,"abstract":"<div><div>Hypertension is one of the most common factors contributing to cardiac remodeling that links to changes in both heart and kidney functions. Several studies have reported the beneficial effects of Coenzyme Q₁₀ (CoQ₁₀) in reducing oxidative stress due to its antioxidant properties. However, the cardioprotective and nephroprotective roles of CoQ₁₀ against the toxicity induced by fludrocortisone acetate (FCA) have yet to be investigated. Therefore, we evaluated the CoQ₁₀ effects on FCA-induced cardiotoxicity and nephrotoxicity in a rat model (FCA-Salt). We analyzed various oxidative stress parameters and antioxidant enzyme activities using biochemical assays. In addition, we examined inflammatory and fibrosis via histological staining of heart and kidney tissues. CoQ₁₀ significantly reduced lipid peroxidation product-malondialdehyde (MDA) while increased nitric oxide (NO) in the FCA-Salt rats. Antioxidant enzyme activities were also enhanced by CoQ₁₀ in these rats. Histopathological examination detected tissue damage and fibrosis in the FCA-Salt group, which was significantly reduced by CoQ₁₀. Furthermore, enzyme-linked immunosorbent assay (ELISA) indicated the activation of Transforming growth factor-β1/Tumor necrosis factor-α (TGF-β1/TNF-α) by CoQ₁₀. Consequently, our study suggests that CoQ₁₀ can effectively protect heart and kidney health against cardiotoxicity and nephrotoxicity in FCA-Salt hypertensive rats via TGF-β1/TNF-α pathway.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110444"},"PeriodicalIF":3.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Mn-superoxide dismutase acetylation protects from enzyme nitration and inactivation","authors":"Gianfranco Frattini ,&nbsp;Juan L. Puzzolo ,&nbsp;Salvador I. Drusin ,&nbsp;Santiago Mansilla ,&nbsp;Rafael Radi ,&nbsp;Verónica Demicheli ,&nbsp;Diego M. Moreno","doi":"10.1016/j.abb.2025.110439","DOIUrl":"10.1016/j.abb.2025.110439","url":null,"abstract":"<div><div>Manganese superoxide dismutase (MnSOD) is a critical enzyme responsible for detoxifying superoxide radicals in mitochondria, thereby ensuring oxidative balance within cells. Post-translational modifications (PTMs), such as acetylation and nitration, significantly influence MnSOD's catalytic efficiency. Site-specific nitration of MnSOD at tyrosine 34 by peroxynitrite leads to an irreversible inactivation, which has been widely observed in diverse pathologies. On the other hand, acetylation of MnSOD is a reversible modification that modulates the activity of the enzyme and it is finely regulated by the action of the protein Sirt3, responsible for the deacetylation of a wide variety of mitochondrial enzymes. This study focuses on Lys29 acetylation and its impact on the enzyme's activity and its interplay with peroxynitrite-mediated nitration of Tyr34. Through molecular dynamics (MD) simulations, we observed that acetylation of Lys29 partially obstructs the access channel to the active site, reducing superoxide accessibility. Electrostatic potential calculations further revealed that Lys29 acetylation diminishes the positive charge around the active site, contributing to decreased affinity for superoxide radicals. Brownian dynamics (BD) simulations confirmed a 50 % reduction in the enzyme's association rate constant (k_on) for superoxide upon Lys29 acetylation. In contrast, Lys98 acetylation had a minor effect on k_on. <em>In vitro</em> studies also supported our findings and showed that acetylation could play a role in the irreversible inactivation of MnSOD by peroxynitrite, likely by sterically hindering Tyr34 nitration. These findings highlight the role of acetylation as a reversible protective mechanism that can regulate superoxide and peroxynitrite accessibility to MnSOD under stress conditions.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110439"},"PeriodicalIF":3.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NFAT2 promotes sorafenib resistance in hepatocellular carcinoma cells by modulating calcium ion signalling","authors":"Jian Wang ,&nbsp;Yi Bai ,&nbsp;Xian-Yi Liu ,&nbsp;Shuang Li ,&nbsp;Ying Wang ,&nbsp;Ya-Min Zhang ,&nbsp;Zhang-Yong Hong","doi":"10.1016/j.abb.2025.110440","DOIUrl":"10.1016/j.abb.2025.110440","url":null,"abstract":"<div><div>Treating hepatocellular carcinoma (HCC) remains challenging due to the drug resistance of HCC cells, which limits the clinical efficacy of sorafenib. This study elucidates the role of nuclear factor activated T cells 2 (NFAT2) in sorafenib resistance in HCC cells and reveals the underlying mechanism. Sorafenib-resistant cell lines were constructed, with NFAT2 overexpressed and knocked down via genetic engineering. Fura-2 detected intracellular calcium ion concentration; transmission electron microscopy (TEM) assessed organelle damage; wound healing and transwell assays evaluated cell migration and invasion; clone formation and CCK8 assays measured cell proliferation. Flow cytometry detected apoptosis; Western blot analyzed protein expressions. Tumorigenesis was evaluated using a sorafenib-resistant HCC orthotopic xenograft mouse model. We found that NFAT2 was upregulated in MHCC97H-SR and HepG2-SR cells. Overexpression of NFAT2 inhibited Ca²⁺ influx in MHCC97H-SR, reduced the expression of Ca²⁺ regulation-related proteins (<em>p</em>-PLCγ, p-IP3R, <em>p</em>-CaMKII), ER-related proteins (CPR94, CPR78), and oxidative stress-related proteins (NOX2, NOX4). NFAT2 overexpression inhibited apoptosis and enhanced cell migration, invasion, and proliferation. NFAT2 knockdown reduced tumorigenesis. Our study uncovered a mechanism by which NFAT2 increases HCC cell resistance to sorafenib by altering intracellular calcium ion signals, highlighting NFAT2 as a promising target for HCC drug therapy.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110440"},"PeriodicalIF":3.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse β-bungarotoxin isoforms manifest different affinities to voltage-gated potassium channels of Kv1.x subfamily","authors":"Ksenia Kudryashova ,&nbsp;Ekaterina Filippova ,&nbsp;Elena Kryukova ,&nbsp;Oleg Kazakov ,&nbsp;Rustam Ziganshin ,&nbsp;Vladislav Starkov ,&nbsp;Oksana Nekrasova ,&nbsp;Victor Tsetlin ,&nbsp;Alexey Feofanov ,&nbsp;Yuri Utkin","doi":"10.1016/j.abb.2025.110437","DOIUrl":"10.1016/j.abb.2025.110437","url":null,"abstract":"<div><div>β-Bungarotoxins (β-BuTx), consisting of covalently bound phospholipase A₂ subunit (A-chain), a member of group Ia of secretory phospholipases A₂, and non-enzymatic subunit (B-chain) structurally related to Kunitz-type protease inhibitors, block presynaptic neuromuscular transmission via a not completely defined mechanism of action. <em>In vivo</em> physiological studies revealed that the B-chain is targeting voltage-gated potassium channels of not identified subtypes. In our work, six β-BuTx isoforms were isolated from <em>Bungarus multicinctus</em> krait venom and characterized by mass spectrometry revealing that isoforms differ in the A- and B-chain composition. Their secondary structures determined by a circular dichroism spectroscopy were similar, while phospholipase activities differed by 1.4–2.6 times between isoforms. The β-BuTx isoforms were found to bind to the extracellular pore blocker binding site of human Kv1.1, Kv1.3 and Kv1.6 channels with submicromolar and micromolar affinities depending on the type of constituent chains. Electrophysiology data demonstrated the ability of β-BuTx isoforms to block human Kv1.3 channels with different efficiency. Though the properties of β-BuTx can depend on their subunit composition, the presented data identify human Kv1.1, Kv1.3 and Kv1.6 channels as pharmacological targets for the particular β-BuTx isoforms.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110437"},"PeriodicalIF":3.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct structural basis and catalytic classification of matrix metalloproteinases and their endogenous tissue inhibitors with glycosylation issue in cellular and tissue regulation","authors":"Hee-Do Kim ,&nbsp;Hyunju Choi ,&nbsp;Jun-Young Park ,&nbsp;Cheorl-Ho Kim","doi":"10.1016/j.abb.2025.110436","DOIUrl":"10.1016/j.abb.2025.110436","url":null,"abstract":"<div><div>Matrix metalloproteinase (MMP) enzymes cleave proteins on the extracellular matrix (ECM) region. MMPs are categorized as Zn²⁺-binding endo-proteinases. MMPs are stringently regulated in cancers, inflammatory cells and tissues. There are 29 types of MMPs as initially expressed in inactive zymogens (proMMPs) and activated by proteolysis in vertebrates including human. MMPs consist of three highly conserved parts of pro-MMP in precursor, catalytic and hemopexin domains. The MMPs are composed of systemic complexes with their endogenously expressed inhibitors of the tissue inhibitors of metalloproteinases (TIMPs). Therefore, TIMPs intrinsically control such activated MMPs, indicating the existence of self-modulation capacity. <em>N</em>-linked glycosylation (<em>N</em>-glycosylation) saves biological information than known phosphorylation, ubiquitination and acetylation. The MMPs are roughly present as membrane-merged and secreted glycoproteins. MMPs <em>N</em>-glycans regulate cellular behaviors, immune tolerance, and developing angiogenesis. Aberrant <em>N</em>-glycosylation of MMPs may cause the pathogenic properties. <em>N</em>-glycosylation shapes phenotypes of MMPs-producing cells during early MMPs involved in human. Additionally, issues of MMPs and TIMPs glycosylation have been described to view the importance of the glycans in their interaction with owns and other targets. Most of MMPs and 4 TIMPs are not well studied for their glycosylation and its functional roles.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110436"},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow mesenchymal stem cells-derived exosomal miR-24–3p alleviates spinal cord injury by targeting MAPK9 to inhibit the JNK/c-Jun/c-Fos pathway","authors":"Dailong Li ,&nbsp;Xuyuan Xie ,&nbsp;Yuxuan Ou ,&nbsp;Peiwen Sun ,&nbsp;Jiezhao Lin ,&nbsp;Cheng Yu ,&nbsp;Haoran Huang ,&nbsp;Longcheng Huang ,&nbsp;Changjian Yang ,&nbsp;Wenhao Kuang ,&nbsp;Chusong Zhou","doi":"10.1016/j.abb.2025.110434","DOIUrl":"10.1016/j.abb.2025.110434","url":null,"abstract":"<div><div>Spinal cord injury (SCI) is a very harmful neurological disease that can cause serious damage to sensation, movement, and autonomic nervous function below the affected area. Apoptosis and inflammatory response play important roles in the pathological process of spinal cord injury. The exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) may play a protective role against spinal cord injury. However, the detailed mechanism behind this is not fully understood. The main objective of this study was to investigate the anti-inflammatory and anti-apoptotic effects of bone marrow mesenchymal stem cell exosomes (BMSCs-EXO) in SCI in vitro and in vivo and their mechanisms. The study demonstrated that bone marrow mesenchymal stem cells reduced apoptosis and inflammation and promoted axon growth in LPS-treated PC12 cells. The miRDB predicted that miR-24–3p targets MAPK9(JNK2). Transcriptome sequencing and Western blot confirmed that miR-24–3p inhibits the JNK/c-Jun/c-Fos pathway by targeting MAPK9. In vivo experiments, injection of BMSC exosomes overexpressing miR-24–3p from the tail vein attenuated the SCI exercise injury-related behavior in rats. In conclusion, this study indicates that bone marrow MSC-derived exosomes can mitigate SCI-related injury by suppressing apoptosis and inflammation, with miR-24–3p playing a crucial role, potentially offering a novel therapeutic approach for SCI treatment.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110434"},"PeriodicalIF":3.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMPK-YAP signaling pathway-mediated mitochondrial dynamics and mitophagy participate in the protective effect of silibinin on HaCaT cells under high glucose conditions","authors":"Xianshi Wang ,&nbsp;Xueying Sun ,&nbsp;Xiaodi Zhang ,&nbsp;Naiying Shen ,&nbsp;Junlong Xia ,&nbsp;Lu Wang ,&nbsp;Shasha Ye","doi":"10.1016/j.abb.2025.110433","DOIUrl":"10.1016/j.abb.2025.110433","url":null,"abstract":"<div><div>UVB irradiation and diabetes lead to skin injury. However, UVB irradiation has rarely been studied in the field of diabetes. Silibinin has a positive therapeutic effect on many diseases. Nevertheless, the inhibitory effects of silibinin on UVB-induced damage to epidermal cells under high glucose (HG) conditions have been infrequently investigated. Consequently, this study examined the protective efficacy and mechanisms of silibinin in mitigating UVB-induced apoptosis in epidermal cells cultured under HG conditions. The effects of combination of HG and UVB on mitochondrial apoptosis and pro-inflammatory factors production in human immortalized keratinocytes (HaCaT) were mitigated by silibinin. Meantime, silibinin reversed the UVB-induced imbalance of fission/fusion in HG-cultured HaCaT cells. Furthermore, UVB exposure increased ROS levels and reduced mitophagy in HaCaT cells under HG conditions; however, these effects were subsequently reversed by silibinin treatment. AMPK preserves energy balance by negatively regulating YAP. Silibinin increased the levels of p-AMPK and cytoplasmic YAP proteins in HaCaT cells subjected to HG and UVB treatment. Moreover, silibinin improved the dysfunction of mitochondrial dynamics, increased mitophagy levels, the viability and the expression of cytoplasmic YAP protein, and these effects were reversed via the application of an AMPK inhibitor (compound C). In summary, silibinin safeguarded epidermal cells from UVB-induced apoptosis under HG conditions by modulating mitochondrial dynamics and mitophagy through the AMPK-YAP signaling pathway.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110433"},"PeriodicalIF":3.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium-dependent levels of phospholamban pentamer in native heart membranes reflect interactions of monomers with calcium-free SERCA2a","authors":"Jin Guo ,&nbsp;Kazuhiro Aonuma,&nbsp;Richard Kovacs,&nbsp;Zhenhui Chen","doi":"10.1016/j.abb.2025.110432","DOIUrl":"10.1016/j.abb.2025.110432","url":null,"abstract":"<div><h3>Background</h3><div>In sarcoplasmic reticulum (SR) membranes, phospholamban (PLB) exists in an equilibrium of non-inhibitory homopentamers (PLB₅) and inhibitory monomers (PLB₁) that bind to SERCA2a. A new approach is needed to determine the full scheme of interactions between PLB and SERCA2a in native cardiac SR membranes, which remains poorly understood.</div></div><div><h3>Methods</h3><div>Dog cardiac SR membranes (dSR) were switched between EGTA and Ca²⁺ buffers to convert SERCA2a between the Ca²⁺-free, <em>E</em>2 and the high Ca²⁺-affinity, <em>E</em>1 conformations. Reactions were stopped by SDS to preserve PLB₅ structures in dSR before immunoblotting.</div></div><div><h3>Results</h3><div>Converting SERCA2a from <em>E</em>2 to <em>E</em>1, Ca²⁺ addition significantly increased PLB₅/PLB₁ ratios, suggesting that PLB₁ is dissociated from <em>E</em>1, and assembled into PLB₅ in dSR. This Ca²⁺-induced increase in PLB₅/PLB₁ was reversed by the subsequent addition of EGTA, revealing the processes of PLB₁ binding to <em>E</em>2 and disassembly of PLB₅. In both cases, PLB₅/PLB₁ reached new steady states in &lt;2 s. Furthermore, PLB antibody eliminated Ca²⁺-dependent shifts in PLB₅/PLB₁. PLB phosphorylation caused similar leftward shifts in the Ca²⁺-dependent curve for PLB₅/PLB₁ and Ca²⁺-ATPase activity.</div></div><div><h3>Conclusions</h3><div>We have developed a simple, effective method and revealed that the levels of SERCA2a inhibition are controlled by an equilibrium between PLB₁ association with <em>E</em>2 and its dissociation from <em>E</em>1, and the formation of PLB₅ in native cardiac SR membranes. With intact regulatory components in their natural phospholipid environment, Ca²⁺-dependent shifts in PLB₅/PLB₁ can expose PLB-SERCA2a protein-protein interactions in native membranes from normal and diseased hearts, in which proteomes and lipidomes are likely to vary.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110432"},"PeriodicalIF":3.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of pathogenic mutations on the refolding ability and stability of human mitochondrial Phenylalanyl-tRNA synthetase","authors":"Debraj Roy ,&nbsp;Shubhangini Singh Verma ,&nbsp;Shruti Chakraborty ,&nbsp;Debkanya Dey ,&nbsp;Surajit Tudu ,&nbsp;Riya Manna ,&nbsp;Nitin Chaudhary ,&nbsp;Rajat Banerjee","doi":"10.1016/j.abb.2025.110430","DOIUrl":"10.1016/j.abb.2025.110430","url":null,"abstract":"<div><div>Human mitochondrial phenylalanyl-tRNA synthetase (hmtPheRS) requires partial unfolding for mitochondrial import and subsequent refolding to maintain proper functionality. Mutations in the FARS2 gene, encoding hmtPheRS, cause disorders such as early-onset epileptic encephalopathy and spastic paraplegia. This study was intended to investigate the impact of mutations on hmtPheRS refolding ability, stability, and solubility. We have selected two mutations associated with early-onset epileptic encephalopathy (G309S, D325Y) with severe phenotype and three mutations associated with spastic paraplegia (P136H, D142Y, P361L) with less severe phenotypes. Some of those mutations were reported to have diminished aminoacylation activity. However, the molecular connection of pathogenicity remained elusive for these mutants. We observed that hmtPheRS showed exceptional structural flexibility and refolding ability even at lower pH. Mutations associated with severe phenotypes (G309S, D325Y) exhibited impaired refolding ability and stability, whereas other mutant versions of hmtPheRS linked to hereditary spastic paraplegia (P136H, D142Y, P361L) retained some stability and refolding capacity. Mutants exhibited expansion in hydrodynamic diameter, indicating significant perturbation in the internal architecture. Molecular simulation studies suggested the presence of structural deformities in hmtPheRS mutants at mildly acidic pH. This analysis reveals how mutations affect protein stability and function, which may play a role in mitochondrial disorders. It may act as a probable model for predicting pathogenicity-related mutants.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"769 ","pages":"Article 110430"},"PeriodicalIF":3.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}