Archives of biochemistry and biophysics最新文献

Molecular characterization of the E2 conjugating enzyme LinfUbc13 in Leishmania infantum. 婴儿利什曼原虫中 E2 连接酶 LinfUbc13 的分子特征。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-12-15 DOI: 10.1016/j.abb.2024.110272

Eduardo Vagner Rodrigues da Silva, Caroline Torres, Hariel Nemamiah Escolarique Ribeiro, Camila Rolemberg Santana Travaglini Berti de Correia, Taissa de Oliveira de Castro, Giovanna da Costa Mancin, Mayla Gabriela Zanchetta Venancio, Munira Muhammad Abdel Baqui, Felipe Roberti Teixeira, Marcelo Damário Gomes

{"title":"Molecular characterization of the E2 conjugating enzyme LinfUbc13 in Leishmania infantum.","authors":"Eduardo Vagner Rodrigues da Silva, Caroline Torres, Hariel Nemamiah Escolarique Ribeiro, Camila Rolemberg Santana Travaglini Berti de Correia, Taissa de Oliveira de Castro, Giovanna da Costa Mancin, Mayla Gabriela Zanchetta Venancio, Munira Muhammad Abdel Baqui, Felipe Roberti Teixeira, Marcelo Damário Gomes","doi":"10.1016/j.abb.2024.110272","DOIUrl":"10.1016/j.abb.2024.110272","url":null,"abstract":"UBC13 is an orthologue of Homo sapiens ubiquitin-conjugation E2 enzymes described in Leishmania mexicana, a null mutant lacking this gene cannot be produced, suggesting essential functions in this parasite. Leishmania infantum is an etiological agent of visceral leishmaniasis, the most severe type of disease that is potentially fatal if untreated. The ubiquitination process has been targeted for leishmanicidal compounds, indicating its essential function in parasite homeostasis. Therefore, the molecular characterization of the ubiquitination process may provide a better understanding of the molecular and cellular basis of leishmaniasis. Here, we characterized the gene LINF_350017900 in Leishmania infantum, which was named LinfUBC13, an E2 orthologue of UBC13 in Leishmania mexicana and the UBE2D family in Homo sapiens, sharing 72-74 % identity with UBE2D1, UBE2D2, and UBE2D3. LinfUbc13 contains conserved catalytic residues, including Cys86 and the HPN motif, which are essential for ubiquitin-conjugating activity. Structural analysis revealed a high similarity between LinfUbc13 and human UBE2D proteins, with a root-mean-square deviation (RMSD) of 0.4 Å, suggesting conserved functions. Recombinant LinfUbc13 was expressed and shown to accept ubiquitin from E1, forming a thioester intermediate. Functional assays demonstrated that LinfUbc13 transfers ubiquitin to p53 through human HDM2 E3 ligase, confirming its role in ubiquitination. Subcellular localization showed that LinfUbc13 was distributed throughout the parasite cytoplasm. These findings highlight the conserved nature of the ubiquitin-proteasome system between Leishmania infantum and Homo sapiens, showing that LinfUbc13 is an E2 enzyme that plays a crucial role in parasitic development.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110272"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of SMAD7 targeting miRNA in the pathogenesis of diabetic nephropathy. 靶向miRNA的SMAD7在糖尿病肾病发病机制中的治疗潜力

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-12-10 DOI: 10.1016/j.abb.2024.110265

V Pooja Rathan, K Bhuvaneshwari, G Nideesh Adit, S Kavyashree, N Thulasi, A V S Geetha, K L Milan, K M Ramkumar

{"title":"Therapeutic potential of SMAD7 targeting miRNA in the pathogenesis of diabetic nephropathy.","authors":"V Pooja Rathan, K Bhuvaneshwari, G Nideesh Adit, S Kavyashree, N Thulasi, A V S Geetha, K L Milan, K M Ramkumar","doi":"10.1016/j.abb.2024.110265","DOIUrl":"10.1016/j.abb.2024.110265","url":null,"abstract":"Diabetic nephropathy (DN) is a common complication of diabetes and a leading cause of end-stage renal disease, characterized by progressive kidney fibrosis and inflammation. The transforming growth factor-beta (TGF-β) signaling pathway plays a crucial role in the pathogenesis of diabetes nephropathy, and SMAD7 is a key negative regulator of this pathway. Recent studies have highlighted the involvement of miRNA in the progression of DN. Computational analysis identified 11 potential miRNAs such as miR-424, miR-195, miR-216a, miR-503, miR-15a-5p, miR-15b-5p, miR-665, miR-520h, miR16-5p, miR-21 and miR-32-5p which are predicted to target 3'UTR of SMAD7 mRNA. This review aims to explore the role of these miRNAs in the progression of DN. Notably, these miRNAs have shown therapeutic potential in mitigating fibrosis and inflammation by modulating SMAD7 expression in DN. Future directions can be to investigate the mechanistic pathways through which these miRNAs exert their effects, as well as optimizing delivery systems for effective clinical application. Targeting miRNAs that modulate SMAD7 expression represents a promising strategy for developing specific and effective therapies for diabetic nephropathy.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110265"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to "Inhibition of carnitine palmitoyl transferase 1A-induced fatty acid oxidation suppresses cell progression in gastric cancer" [Archiv. Biochem. Biophys. 696 (2020) 108664]. “抑制肉碱棕榈酰转移酶1a诱导的脂肪酸氧化抑制胃癌细胞进展”的撤回通知[文献]。物化学。生物化学学报，2016,32(1):464 - 464。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-12-11 DOI: 10.1016/j.abb.2024.110264

Liqiang Wang, Changfeng Li, Yumei Song, ZhenKun Yan

引用次数: 0

Structural and biophysical characterization of the cytoplasmic domains of HprS kinase and its interactions with the cognate regulator HprR. HprS激酶细胞质结构域的结构和生物物理特性及其与同源调节因子HprR的相互作用。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-12-15 DOI: 10.1016/j.abb.2024.110269

Anna Koczurowska, David Ruiz Carrillo, María García Alai, Małgorzata Zakłos-Szyda, Grzegorz Bujacz, Agnieszka J Pietrzyk-Brzezinska

{"title":"Structural and biophysical characterization of the cytoplasmic domains of HprS kinase and its interactions with the cognate regulator HprR.","authors":"Anna Koczurowska, David Ruiz Carrillo, María García Alai, Małgorzata Zakłos-Szyda, Grzegorz Bujacz, Agnieszka J Pietrzyk-Brzezinska","doi":"10.1016/j.abb.2024.110269","DOIUrl":"10.1016/j.abb.2024.110269","url":null,"abstract":"The HprSR constitutes the bacterial two-component regulatory system engaged by Escherichia coli to reduce the damaging effects of reactive chlorine and oxygen species present in its cytosol. Hypochlorous acid (HOCl) has been shown to be the molecule capable of activating of the HprSR system. HOCl is produced upon pathogen invasion by phagocytic cells of the human innate immune system, particularly neutrophils, to take advantage of its powerful antimicrobial attributes. Therefore, comprehensive studies concerning bacterial sensing and regulatory HprSR system are indispensable in understanding and effectively eliminating pathogens. Here we present the first crystal structure, solved at 1.7 Å resolution, of the HprS cytoplasmic domains arranged as a homodimer. In both protomers, the catalytic ATP-binding domain contains a non-hydrolysable ATP analog coordinated by a magnesium ion. This structure allowed us to provide a detailed characterization of kinase-substrate interaction. Furthermore, the structural data are supported by biophysical studies of kinase interaction with cognate response regulator HprR and substrate ATP. The kinase activity is also assessed in the presence or absence of HprR.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110269"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ganoderic acid a potential protective impact on bleomycin (BLM) -induced lung fibrosis in albino mice: Targeting caveolin 1/TGF-β/ Smad and P38MAPK signaling pathway. 灵芝酸对博来霉素（BLM）诱导的白化小鼠肺纤维化的潜在保护作用：靶向Caveolin 1/TGF-β/ Smad和P38MAPK信号通路

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-12-29 DOI: 10.1016/j.abb.2024.110284

Amira M Elshamy, Asmaa F El Tantawy, Eman H Basha, Eman F Eltabaa, Heba M Arakeeb, Ahmed S Ahmed, Amal M Abdelsattar, Rowida Raafat Ibrahim, Omnia Safwat El Deeb, Asmaa M Eid, Shaimaa S Mashal, Mohamed A Safa, Amany Mohamed Shalaby, Hoda A Ibrahim

{"title":"Ganoderic acid a potential protective impact on bleomycin (BLM) -induced lung fibrosis in albino mice: Targeting caveolin 1/TGF-β/ Smad and P38MAPK signaling pathway.","authors":"Amira M Elshamy, Asmaa F El Tantawy, Eman H Basha, Eman F Eltabaa, Heba M Arakeeb, Ahmed S Ahmed, Amal M Abdelsattar, Rowida Raafat Ibrahim, Omnia Safwat El Deeb, Asmaa M Eid, Shaimaa S Mashal, Mohamed A Safa, Amany Mohamed Shalaby, Hoda A Ibrahim","doi":"10.1016/j.abb.2024.110284","DOIUrl":"10.1016/j.abb.2024.110284","url":null,"abstract":"Background: Bleomycin (BLM), an anticancer medication, can exacerbate pulmonary fibrosis by inducing oxidative stress and inflammation. Anti-inflammatory, anti-fibrotic, and antioxidant properties are exhibited by ganoderic acid A (GAA).Aim: So, we aim to assess GAA's protective impact on lung fibrosis induced via BLM.Method: Forty mice were randomly classified into four groups. Lung fibrosis was induced by injection of BLM intraperitoneally (15 mg/kg body weight). GAA was given by oral gavage (25 mg/kg body weight). Lung tissue MDA, TAC, and GSH were assessed spectrophotometrically. As well, TGFβ, p38 MAPK, TNF-α, IL-1β, and CAV1 levels were measured by enzyme-linked immunosorbent assay. Gene expression of tumor growth factor beta (TGF-β), Smad2, Smad3, and glutamate-cysteine ligase (GCL) were also evaluated.Results: GAA had significantly improved biochemical biomarkers as well as histopathology of the lung. The protective impact of GAA may be linked to the upregulation of GCL gene expression and subsequent GSH levels. In addition, the GAA-treated group showed a significant decrement in the levels of TGF-β, Smad2&3, P38 MAPK, TNF-α, IL1β, and MDA compared to BLM induced lung fibrosis group. GAA has a protective impact on lung fibrosis induced by BLM via downregulation of TGF-β and upregulation of CAV1 level and GCL expression which may play a critical role in the improvement of the pathogenesis of lung fibrosis induced via BLM.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110284"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytic insights of acetolactate synthases from different bacteria. 不同细菌对乙酰乳酸合酶的催化作用。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-11-29 DOI: 10.1016/j.abb.2024.110248

Yan-Fei Liang, Ze-Xin Niu, Zi-Wen Wu, Qing-Yang Zhang, Xin-Yi Zhao, Lei-Lei Chao, Heng Li, Wen-Yun Gao

{"title":"Catalytic insights of acetolactate synthases from different bacteria.","authors":"Yan-Fei Liang, Ze-Xin Niu, Zi-Wen Wu, Qing-Yang Zhang, Xin-Yi Zhao, Lei-Lei Chao, Heng Li, Wen-Yun Gao","doi":"10.1016/j.abb.2024.110248","DOIUrl":"10.1016/j.abb.2024.110248","url":null,"abstract":"Acetolactate synthase (ALS) is an essential enzyme involved in the biosynthesis of platform chemicals acetoin and 2,3-butanediol in several microorganisms. In this study, we investigated the catalytic differences among three bacterial ALSs involved in the ligation of two molecules of pyruvate or 2-ketobutyrate. Based on the findings, we predicted three amino acid residues in each enzyme that caused a discrepancy in accordance with the multi-sequence alignment and molecular docking experiments: I398, A402, and T480 in Bacillus subtilis ALS; V400, Y404, and S482 in Listeria seleigeri serovar 1/2b ALS; and M394, H398, and G476 in Klebsiella pneumoniae ALS. Subsequently, we mutually mutated the residues in the three ALSs. The data obtained confirmed our inference that these three residues in each enzyme are truly correlated with substrate recognition, particularly in recognizing compounds that are larger than pyruvate, such as 2-ketobutyrate, benzaldehyde, and nitrosobenzene. This study further clarifies the biochemical traits of ALSs derived from various bacteria and expands the scope of ALS research.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110248"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The importance of helical structures to the overall activity and structural stability of a lipase from Pseudomonas aeruginosa PAO1 in n-hexane. 螺旋结构对正己烷中铜绿假单胞菌PAO1脂肪酶的总体活性和结构稳定性的重要性。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-11-29 DOI: 10.1016/j.abb.2024.110226

Jaidriel Meg G Cabanding, Steve S-F Yu, Zhi-Han Lin, Myrnel A Fortuna, Adam Jo J Elatico, Ricky B Nellas

{"title":"The importance of helical structures to the overall activity and structural stability of a lipase from Pseudomonas aeruginosa PAO1 in n-hexane.","authors":"Jaidriel Meg G Cabanding, Steve S-F Yu, Zhi-Han Lin, Myrnel A Fortuna, Adam Jo J Elatico, Ricky B Nellas","doi":"10.1016/j.abb.2024.110226","DOIUrl":"10.1016/j.abb.2024.110226","url":null,"abstract":"Bacterial lipases are versatile extracellular enzymes with a catalytic triad at the active site and a flexible 'lid' that modulates catalytic accessibility. We combined computational modeling with preliminary in vitro testing to assess the structural stability and activity of the Pseudomonas aeruginosa PAO1 lipase (PAL). We evaluated several systems consisting of the native and mutant forms of the lipase in n-hexane using molecular dynamics simulations. Structural stability was assessed by calculating the B-factor for each system. We measured the gorge radius of the catalytic channel and the RMSD of the catalytic triad to approximate enzymatic activity. Based on the correlation of these metrics, mutant forms were selected for their potential activity and stability. Selected mutant forms were expressed in E. coli BL21, mass-produced, and validated through a lipase-catalyzed esterification assay in n-hexane. Several helices outside the 'lid' region were found to influence lid conformational switching. Moreover, our preliminary experimental results show promise in validating our in silico predictions. Our integrated in silico and in vitro pipeline offers a promising approach for designing and producing industrially relevant lipases.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110226"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New magnetic iron nanoparticle doped with selenium nanoparticles and the mechanisms of their cytoprotective effect on cortical cells under ischemia-like conditions. 磁性铁纳米粒子掺杂硒纳米粒子及其对脑缺血样皮质细胞的保护作用机制。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-11-28 DOI: 10.1016/j.abb.2024.110241

Egor A Turovsky, Egor Y Plotnikov, Alexander V Simakin, Sergey V Gudkov, Elena G Varlamova

{"title":"New magnetic iron nanoparticle doped with selenium nanoparticles and the mechanisms of their cytoprotective effect on cortical cells under ischemia-like conditions.","authors":"Egor A Turovsky, Egor Y Plotnikov, Alexander V Simakin, Sergey V Gudkov, Elena G Varlamova","doi":"10.1016/j.abb.2024.110241","DOIUrl":"10.1016/j.abb.2024.110241","url":null,"abstract":"Ischemic stroke is the cause of high mortality and disability Worldwide. The material costs of stroke treatment and recovery are constantly increasing, making the search for effective and more cost-effective treatment approaches an urgent task for modern biomedicine. In this study, iron nanoparticles doped with selenium nanoparticles, FeNP@SeNPs, which are three-layered structures, were created and characterized using physical methods. Fluorescence microscopy, inhibitor and PCR analyzes were used to determine the signaling pathways involved in the activation of the Ca2+ signaling system of cortical astrocytes and the protection of cells from ischemia-like conditions (oxygen-glucose deprivation and reoxygenation). In particular, when using magnetic selenium nanoparticles together with electromagnetic stimulation, an additional pathway for nanoparticle penetration into the cell is activated through the activation of TRPV4 channels and the mechanism of their endocytosis is facilitated. It has been shown that the use of magnetic selenium nanoparticles together with magnetic stimulation represents an advantage over the use of classical selenium nanoparticles, as the effective concentration of nanoparticles can be reduced many times over.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110241"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrolysis of the acetyl-CoA allosteric activator by Staphylococcus aureus pyruvate carboxylase. 金黄色葡萄球菌丙酮酸羧化酶水解乙酰辅酶a变构激活剂。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-12-24 DOI: 10.1016/j.abb.2024.110280

Amanda J Laseke, Jeremy R Lohman, Martin St Maurice

{"title":"Hydrolysis of the acetyl-CoA allosteric activator by Staphylococcus aureus pyruvate carboxylase.","authors":"Amanda J Laseke, Jeremy R Lohman, Martin St Maurice","doi":"10.1016/j.abb.2024.110280","DOIUrl":"10.1016/j.abb.2024.110280","url":null,"abstract":"Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate which serves as an important anaplerotic reaction to replenish citric acid cycle intermediates. In most organisms, the PC-catalyzed reaction is allosterically activated by acetyl-coenzyme A. It has previously been reported that vertebrate PC can catalyze the hydrolysis of acetyl-CoA, offering a potential means for the enzyme to attenuate its allosteric activation. However, in the years since this initial report, there has been no further investigation of this phenomenon. The allosteric binding site for acetyl-CoA is now well characterized, enabling more detailed studies on acetyl-CoA hydrolysis at the allosteric site. Here, we confirm that slow acetyl-CoA hydrolysis is catalyzed by a bacterial PC from Staphylococcus aureus, indicating that this phenomenon is a broad feature of PC enzymes spanning the domains of life. Surprisingly, the enzyme can hydrolyze acetyl-CoA even when the binding site for the acetyl moiety is eliminated through truncation of the biotin carboxylase domain. This suggests that an alternative site for acetyl-CoA binding and hydrolysis may be present in the carboxyltransferase domain of S. aureus PC. We conclude that PC has evolved to minimize the rate of acetyl-CoA hydrolysis at the allosteric site and update the description of PC-catalyzed acetyl-CoA hydrolysis to suggest that this reaction is unlikely to play a significant physiological, metabolic or catalytic role.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110280"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenoprotein K at the intersection of cellular pathways. 细胞通路交叉点上的硒蛋白 K。

IF 3.8 3区生物学

Archives of biochemistry and biophysics Pub Date : 2025-02-01 Epub Date: 2024-11-20 DOI: 10.1016/j.abb.2024.110221

Atinuke Odunsi, Mariia A Kapitonova, George Woodward, Erfan Rahmani, Farid Ghelichkhani, Jun Liu, Sharon Rozovsky

{"title":"Selenoprotein K at the intersection of cellular pathways.","authors":"Atinuke Odunsi, Mariia A Kapitonova, George Woodward, Erfan Rahmani, Farid Ghelichkhani, Jun Liu, Sharon Rozovsky","doi":"10.1016/j.abb.2024.110221","DOIUrl":"10.1016/j.abb.2024.110221","url":null,"abstract":"Selenoprotein K (selenok) is linked to the integrated stress response, which helps cells combat stressors and regain normal function. The selenoprotein contains numerous protein interaction hubs and post-translational modification sites and is involved in protein palmitoylation, vesicle trafficking, and the resolution of ER stress. Anchored to the endoplasmic reticulum (ER) membrane, selenok interacts with protein partners to influence their stability, localization, and trafficking, impacting various cellular functions such as calcium homeostasis, cellular migration, phagocytosis, gene expression, and immune response. Consequently, selenok expression level is linked to cancer and neurodegenerative diseases. Because it contains the reactive amino acid selenocysteine, selenok is likely to function as an enzyme. However, highly unusual for enzymes, the protein segment containing the selenocysteine lacks a stable secondary or tertiary structure, yet it includes multiple interaction sites for protein partners and post-translational modifications. Currently, the reason(s) for the presence of the rare selenocysteine in selenok is not known. Furthermore, of selenok's numerous interaction sites, only some have been sufficiently characterized, leaving many of selenok's potential protein partners to be discovered. In this review, we explore selenok's role in various cellular pathways and its impact on human health, thereby highlighting the links between its diverse cellular functions.","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":" ","pages":"110221"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0